DE3249557A1 - Apparatus for disposing of combustible media or media hazardous to health after they have been used as coolant in a cooling bath for rapidly cooling samples (immersion cryofixation) for a subsequent optical or electron-optical examination - Google Patents

Apparatus for disposing of combustible media or media hazardous to health after they have been used as coolant in a cooling bath for rapidly cooling samples (immersion cryofixation) for a subsequent optical or electron-optical examination

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Publication number
DE3249557A1
DE3249557A1 DE19823249557 DE3249557A DE3249557A1 DE 3249557 A1 DE3249557 A1 DE 3249557A1 DE 19823249557 DE19823249557 DE 19823249557 DE 3249557 A DE3249557 A DE 3249557A DE 3249557 A1 DE3249557 A1 DE 3249557A1
Authority
DE
Germany
Prior art keywords
media
cooling
cryofixation
coolant
immersion
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Ceased
Application number
DE19823249557
Other languages
German (de)
Inventor
H. Prof. Dr. 6100 Seefeld Sitte
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
C Reichert Optische Werke AG
Original Assignee
C Reichert Optische Werke AG
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by C Reichert Optische Werke AG filed Critical C Reichert Optische Werke AG
Priority to DE19823249557 priority Critical patent/DE3249557A1/en
Priority claimed from DE3234457A external-priority patent/DE3234457C2/en
Priority to DE19833347803 priority patent/DE3347803A1/en
Publication of DE3249557A1 publication Critical patent/DE3249557A1/en
Ceased legal-status Critical Current

Links

Classifications

    • F—MECHANICAL ENGINEERING; LIGHTING; HEATING; WEAPONS; BLASTING
    • F25—REFRIGERATION OR COOLING; COMBINED HEATING AND REFRIGERATION SYSTEMS; HEAT PUMP SYSTEMS; MANUFACTURE OR STORAGE OF ICE; LIQUEFACTION SOLIDIFICATION OF GASES
    • F25D—REFRIGERATORS; COLD ROOMS; ICE-BOXES; COOLING OR FREEZING APPARATUS NOT OTHERWISE PROVIDED FOR
    • F25D3/00—Devices using other cold materials; Devices using cold-storage bodies
    • F25D3/10—Devices using other cold materials; Devices using cold-storage bodies using liquefied gases, e.g. liquid air
    • F—MECHANICAL ENGINEERING; LIGHTING; HEATING; WEAPONS; BLASTING
    • F17—STORING OR DISTRIBUTING GASES OR LIQUIDS
    • F17C—VESSELS FOR CONTAINING OR STORING COMPRESSED, LIQUEFIED OR SOLIDIFIED GASES; FIXED-CAPACITY GAS-HOLDERS; FILLING VESSELS WITH, OR DISCHARGING FROM VESSELS, COMPRESSED, LIQUEFIED, OR SOLIDIFIED GASES
    • F17C13/00—Details of vessels or of the filling or discharging of vessels
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N1/00—Sampling; Preparing specimens for investigation
    • G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
    • G01N1/42—Low-temperature sample treatment, e.g. cryofixation
    • F—MECHANICAL ENGINEERING; LIGHTING; HEATING; WEAPONS; BLASTING
    • F17—STORING OR DISTRIBUTING GASES OR LIQUIDS
    • F17C—VESSELS FOR CONTAINING OR STORING COMPRESSED, LIQUEFIED OR SOLIDIFIED GASES; FIXED-CAPACITY GAS-HOLDERS; FILLING VESSELS WITH, OR DISCHARGING FROM VESSELS, COMPRESSED, LIQUEFIED, OR SOLIDIFIED GASES
    • F17C2205/00—Vessel construction, in particular mounting arrangements, attachments or identifications means
    • F17C2205/03—Fluid connections, filters, valves, closure means or other attachments
    • F17C2205/0302—Fittings, valves, filters, or components in connection with the gas storage device
    • F17C2205/0323—Valves
    • F—MECHANICAL ENGINEERING; LIGHTING; HEATING; WEAPONS; BLASTING
    • F17—STORING OR DISTRIBUTING GASES OR LIQUIDS
    • F17C—VESSELS FOR CONTAINING OR STORING COMPRESSED, LIQUEFIED OR SOLIDIFIED GASES; FIXED-CAPACITY GAS-HOLDERS; FILLING VESSELS WITH, OR DISCHARGING FROM VESSELS, COMPRESSED, LIQUEFIED, OR SOLIDIFIED GASES
    • F17C2221/00—Handled fluid, in particular type of fluid
    • F17C2221/03—Mixtures
    • F17C2221/032—Hydrocarbons
    • F17C2221/035—Propane butane, e.g. LPG, GPL
    • F—MECHANICAL ENGINEERING; LIGHTING; HEATING; WEAPONS; BLASTING
    • F17—STORING OR DISTRIBUTING GASES OR LIQUIDS
    • F17C—VESSELS FOR CONTAINING OR STORING COMPRESSED, LIQUEFIED OR SOLIDIFIED GASES; FIXED-CAPACITY GAS-HOLDERS; FILLING VESSELS WITH, OR DISCHARGING FROM VESSELS, COMPRESSED, LIQUEFIED, OR SOLIDIFIED GASES
    • F17C2223/00—Handled fluid before transfer, i.e. state of fluid when stored in the vessel or before transfer from the vessel
    • F17C2223/01—Handled fluid before transfer, i.e. state of fluid when stored in the vessel or before transfer from the vessel characterised by the phase
    • F17C2223/0146—Two-phase
    • F17C2223/0153—Liquefied gas, e.g. LPG, GPL
    • F—MECHANICAL ENGINEERING; LIGHTING; HEATING; WEAPONS; BLASTING
    • F17—STORING OR DISTRIBUTING GASES OR LIQUIDS
    • F17C—VESSELS FOR CONTAINING OR STORING COMPRESSED, LIQUEFIED OR SOLIDIFIED GASES; FIXED-CAPACITY GAS-HOLDERS; FILLING VESSELS WITH, OR DISCHARGING FROM VESSELS, COMPRESSED, LIQUEFIED, OR SOLIDIFIED GASES
    • F17C2223/00—Handled fluid before transfer, i.e. state of fluid when stored in the vessel or before transfer from the vessel
    • F17C2223/01—Handled fluid before transfer, i.e. state of fluid when stored in the vessel or before transfer from the vessel characterised by the phase
    • F17C2223/0146—Two-phase
    • F17C2223/0153—Liquefied gas, e.g. LPG, GPL
    • F17C2223/0161—Liquefied gas, e.g. LPG, GPL cryogenic, e.g. LNG, GNL, PLNG

Landscapes

  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Mechanical Engineering (AREA)
  • General Engineering & Computer Science (AREA)
  • Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Analytical Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • General Health & Medical Sciences (AREA)
  • General Physics & Mathematics (AREA)
  • Immunology (AREA)
  • Pathology (AREA)
  • Combustion & Propulsion (AREA)
  • Thermal Sciences (AREA)
  • Sampling And Sample Adjustment (AREA)

Abstract

An apparatus for disposing of combustible media or media hazardous to health after they have been used as coolant in a cooling bath for rapidly cooling samples in the immersion cryofixation of biological specimens. In order to be able to eliminate, store or re-use the cryogens, for example liquefied propane, used as such coolants safely, a sealable pressure-resistant container is provided for receiving a container containing the coolant. The pressure container has a connection in which a valve is fitted and which can either be connected to a burner for burning-off or to a liquefier for reliquefying the coolant.

Description

Einrichtung zur Entsorgung von brennbaren oder gesund-Equipment for the disposal of combustible or healthy

heitsschädlichen Medien nach deren Verwendung als Kühlmedium in, einem Kühlbad zum raschen Ahkühlen von Proben (Immersions-Kryofixation) für eine nachfolgende licht-oder elektronenoptische Untersuchung Die Erfindung betrifft eine Einrichtung zur Entsorgung von brennbaren oder gesundheitsschädlichen Medien nach deren Verwendung als Kühlmedium in einem Kühlbad zum raschen Abkühlen von Proben bei der immersions-Kryofixation, insbesondere der Kryofixation biologischer Objekte für eine nachfolgende licht- oder elektronenoptische Untersuchung.harmful media after their use as a cooling medium in one Cooling bath for rapid cooling of samples (immersion cryofixation) for a subsequent one light-optical or electron-optical examination The invention relates to a device for the disposal of flammable or harmful media after their use as a cooling medium in a cooling bath for rapid cooling of samples during immersion cryofixation, especially the cryofixation of biological objects for subsequent light- or electron optical examination.

Für zahl reiche Präparationsarbei.ten im Rahmcn li.cht- oder elektronenoptischer Untersuchungen, insbesondere zum Schockgefrieren biologischer Proben (Immersions-Kryofixation) benötigt man Kühlbäder mit Temperaturen zwischen -100°C und -190°C sowie ein Volumen zwischen 5 und 100 ml. Für derartige Kühlbäder'werden allgemein verflüssigte Gase verwendet, die sich sehr weit unter ihren Siedepunkt abkühlen lassen und nahe ihrem Gefrierpunkt eine relativ hohe Dichte und spezifische Wärme aufweisen. Günstige Eigenschaften hierfür haben u.a. Propan (Fp.-1900C), Freon 13 (Fp.-185-0C) und Isopenthan (Fp.-l600C). Bei der Verwendung dieser brennbaren und/oder gesundheitsschädlichen Kryogene können am Ende der Benutzung Probleme durch das spontane Abdampfen des Küh]mediums in die Raumatmosphäre entstehen. Auch ist es bei teuren Kühlmedien wünschenswert, die Kryogene aufzubewahren, um sie neuerlich abzukühlen und wieder einsetzen zu können.For numerous preparation work, light or electron optical Investigations, especially for shock freezing biological samples (immersion cryofixation) you need cooling baths with temperatures between -100 ° C and -190 ° C and a volume between 5 and 100 ml. Liquefied gases are generally used for such cooling baths used, which can be cooled very far below their boiling point and close to their Freezing point have a relatively high density and specific heat. Cheap Properties for this have inter alia propane (mp -1900C), Freon 13 (mp-185-0C) and isopenthan (M.p. -1600C). When using this flammable and / or harmful to health Cryogens can cause problems at the end of use due to the spontaneous evaporation of the Cooling mediums arise in the room atmosphere. In the case of expensive cooling media, it is also desirable to keep the cryogens in order to cool them down again and to use them again can.

Eine hinreichend eS.Ihrlose Entsorgung dieser Kühlmedien ist jedoch bisher nicht möglich.However, it is sufficient to dispose of these cooling media without them not possible so far.

Der Erfindung liegt deshalb die Aufgabe zugrunde, hier Abhilfe zu schaffen und eine Einrichtung zur Entsorgung vorzuschlagen, die eine gefahrlose Beseitigung oder Aufbewahrung der Kühlmedien erlaubt.The invention is therefore based on the object of remedying this create and propose a disposal facility that is safe Removal or storage of cooling media allowed.

Erfindungsgemäß i'.;t i.st i.ne derartige Einrichtung gekennzeichnet durch einen verschließbaren druckfesten Behälter zur Aufnahme eines das Kühlmedium enthaltenden Behälters, mit einem ein Ventil aufweisenden Anschluß, der mit einem Brenner zum Abfackeln oder mit einem Verflüssiger verbindbar ist.According to the invention i '.; T i.st i.ne such device identified by means of a closable pressure-tight container to hold the cooling medium containing container, with a valve having a connection with a Burner for flaring or can be connected to a condenser.

Der das Kühlmedium enthaltende Behälter kann somit in einen Druckbehälter eingehracht werden, der einen gasdichten Verschluß und ein Ventil zum dosierten Ablassen des gasförmigen Kühlmediums nach dem Erwärmen auf Raumtemperatur aufweist. Die Einrichtung ermöglicht einerseits einen neuerlichen Einsatz des Kühlmediums durch erneute Verflüssigung, andererseits aber auch ein langsames Abfackeln mittels eines Brenners (z.B. eines Propangasbrenners), was die risikolose Beseitigung auch in voll klimatisierten Hochbauten mit durchgehend geschlossenen Fenstern erlaubt, die heute oftmals für Laboratorien verwendet werden.The container containing the cooling medium can thus be placed in a pressure container be einehracht, a gas-tight closure and a valve for metered Having draining the gaseous cooling medium after heating to room temperature. On the one hand, the device enables the cooling medium to be used again through renewed liquefaction, but on the other hand also a slow flaring by means of a burner (e.g. a propane gas burner), which also means risk-free disposal allowed in fully air-conditioned buildings with continuously closed windows, which are now often used in laboratories.

Der das Kühlmedium aufnehmende Behälter kann direkt der Kühlbadbehälter sein, der lösbar mit der gesamten Kühleinrichtung verbunden ist.The container receiving the cooling medium can directly be the cooling bath container be, which is detachably connected to the entire cooling device.

Ein Ausführungsbeispiel der Erfindung ist nachfolgend anhand der beiliegenden Zeichnungen näher erläutert. In den Zeichnungen zeigen: Figur 1 eine schematische Darstellung einer Kühleinrichtung, in der ein lösbar befestigter Kühlbadbehälter zur Aufnahme des Kühlmediums Verwendung findet, und Figur 2 eine schematische Schnittdarstellung eines Behälters zur Aufnahme des Kühlbadbehälters mit angeschlossener Abfackeleinrichtung.An embodiment of the invention is shown below with reference to the enclosed Drawings explained in more detail. In the drawings: FIG. 1 shows a schematic Representation of a cooling device in which a detachably attached cooling bath container to the Recording of the cooling medium is used, and FIG. 2 shows a schematic sectional view of a container for receiving the cooling bath container with connected flaring device.

Bei der in Fig. 1 dargestellten Khleinrichtung ist in einem Behälter 31 ein Kühlhadbehälter 32 zur Aufnahme eines Kühl mediums 6 lösbar angeordnet. Der Behälter 31 befindet sich in einem Topf 7, der wiederum in einem Gefäß 25 aufgenommen ist, welches ein flüssiges Kryogen 4, z.B. flüssigen Stickstoff, enthält. Das Gefäß 25- ist von einer Thermoisolation 26 umgeben. In den Behälter 31 ist außerdem ein Gasverflüssiger 28 integriert, der über einen Anschluß 5 an eine nicht gezeigte Gasflasche, z.B.The cooling device shown in FIG. 1 is in a container 31, a cooling container 32 for receiving a cooling medium 6 is detachably arranged. Of the The container 31 is located in a pot 7, which in turn is accommodated in a vessel 25 which contains a liquid cryogen 4, e.g., liquid nitrogen. The container 25- is surrounded by thermal insulation 26. In the container 31 is also a Integrated gas liquefier 28, which is connected via a connection 5 to a not shown Gas bottle, e.g.

für Propangas, angeschlossen ist.for propane gas.

Über der Kühleinrichtung ist eine Injektorhalterung 40, ein Injektor 41, ein Auslöser 42, ein Montageelement 43 sowie ein Objekthalter 44 mit einem, z.B. biologischen, Objekt 45 angeordnet. Diese Anordnung dient zum Einbringen des Objekts 45 in das Kühlmedium 6 zum Zweck einer Kryofixation des Objekts (vgl. P 32 34 457.0).An injector holder 40, an injector, is located above the cooling device 41, a trigger 42, a mounting element 43 and an object holder 44 with a, e.g. biological, object 45 arranged. This arrangement is used to introduce the Object 45 into the cooling medium 6 for the purpose of cryofixing the object (see P. 32 34 457.0).

Da in vielen moderenen Laborbauten, insbesondere in Hochbauten mit Vollklimatisierung, keine Möglichkeit besteht, kondensiertes Propan oder andere brennbare oder g-esundheitsschädliche Gase risikolos zu Verdampfen, ist zur Entsorgung die in Fig. 2 dargestellte Entsorgungseinrichtung vorgesehcn. Diese weist einen Druckbehälter 34 auf, in welchem der Kühlbadbehälter 32 aufgenommen werden kann, so dnß das Kühmedium 6 risikolos gelagert, wiederverwendet, verbrannt oder ins Freie transportiert werden kann. er Druckbehälter 34 kann nach Einbringen des Kühlbadbehälters 32 mit dem Kühlmedium 6 sowie nach Verschluß mit einem Decke 35 (Dichtungsring 36) und dem Erwärmen ds Kühlmediums 6 auf Raumtemperatur über ein Ventil 37 (z.B. ein handelsübliches Reduzierventil) entleert werden.As in many modern laboratory buildings, especially in high-rise buildings with Full air conditioning, there is no possibility of condensed propane or other Evaporation of flammable or noxious gases without risk is for disposal the disposal device shown in Fig. 2 vorgesehcn. This shows you Pressure vessel 34, in which the cooling bath container 32 can be received, in this way the cooling medium 6 can be stored, reused, burned or released without risk can be transported. he pressure vessel 34 can after introduction of the cooling bath container 32 with the cooling medium 6 and after closure with a cover 35 (sealing ring 36) and heating the cooling medium 6 to room temperature via a valve 37 (e.g. a commercially available reducing valve).

Im geschlossenen Raum können Propan oder andere brennbare Gase risikolos mittels eines angeschlossenen Brenners 38 abgefackelt werden. f)er Druckbehälter 34 kann aber auch erneut an den Ansch]uß 5 zu dem Verflüssiger 28 angeschlossen werden, um das in ihm enthaltene Kühlmedium 6 erneut zu verflüssigen und zu verwenden. Über ein Manometer 39 kann hierbei der E'üllstand kontrolliert werden.Propane or other flammable gases can be used safely in a closed room be flared by means of a connected burner 38. f) he pressure vessel 34 can, however, also be reconnected to the connection 5 to the condenser 28 in order to liquefy and use the cooling medium 6 contained in it again. The filling level can be checked by means of a manometer 39.

Claims (1)

Einrichtung zur Entsorgung von brennbaren oder gesundheitsschädlichen Medien nach deren Verwendung als Kühlmedium in einem Kühlbad zum raschen Abkühlen von Proben (Immersions-Kryofixation) für eine nachfolgende licht-oder elektronenoptische Untersuchung-P a t e n t a n s p r u c h E.inrichtung zur Entsorgung von brennbaren oder gesundheitsschädlichen Medien nach deren Verwendung als Kühlmedium zum raschen Abkühlen von Proben bei der Immersions-Kryofixation, insbesondere der Kryofixation biologischer Objekt, gekennzeichnet durch einen verschließbaren druckfesten Behälter (34) zur Aufnahme eines das Kühlmedium (6) enthaltenden Behälters (32), mit einem ein Ventil (37) aufweisenden Anschluß, der mit einem Brenner (38) zum Ahfackeln oder mit einem Verflüssiger (28) verbindbar ist.Device for the disposal of flammable or harmful Media after their use as a cooling medium in a cooling bath for rapid cooling of samples (immersion cryofixation) for a subsequent light or electron optical Investigation P a t e n t a n s p r u c h E. facility for the disposal of combustible or harmful media after they have been used as a cooling medium Cooling of samples during immersion cryofixation, especially cryofixation biological object, characterized by a lockable pressure-tight container (34) for receiving a container (32) containing the cooling medium (6), with a a valve (37) having a connection with a burner (38) for flaring or can be connected to a condenser (28).
DE19823249557 1982-09-17 1982-09-17 Apparatus for disposing of combustible media or media hazardous to health after they have been used as coolant in a cooling bath for rapidly cooling samples (immersion cryofixation) for a subsequent optical or electron-optical examination Ceased DE3249557A1 (en)

Priority Applications (2)

Application Number Priority Date Filing Date Title
DE19823249557 DE3249557A1 (en) 1982-09-17 1982-09-17 Apparatus for disposing of combustible media or media hazardous to health after they have been used as coolant in a cooling bath for rapidly cooling samples (immersion cryofixation) for a subsequent optical or electron-optical examination
DE19833347803 DE3347803A1 (en) 1982-09-17 1983-09-09 Device for the disposal of media which are flammable or harmful to health, after use thereof as cooling medium, in an immersion cryofixing device

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
DE19823249557 DE3249557A1 (en) 1982-09-17 1982-09-17 Apparatus for disposing of combustible media or media hazardous to health after they have been used as coolant in a cooling bath for rapidly cooling samples (immersion cryofixation) for a subsequent optical or electron-optical examination
DE3234457A DE3234457C2 (en) 1982-09-17 1982-09-17 Cooling bath for rapid cooling of samples, especially for the cryofixation of biological objects for a subsequent light or electron optical examination

Publications (1)

Publication Number Publication Date
DE3249557A1 true DE3249557A1 (en) 1984-10-11

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DE19823249557 Ceased DE3249557A1 (en) 1982-09-17 1982-09-17 Apparatus for disposing of combustible media or media hazardous to health after they have been used as coolant in a cooling bath for rapidly cooling samples (immersion cryofixation) for a subsequent optical or electron-optical examination

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Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE3347803A1 (en) * 1982-09-17 1985-06-20 C. Reichert Optische Werke Ag, Wien Device for the disposal of media which are flammable or harmful to health, after use thereof as cooling medium, in an immersion cryofixing device
DE3332586C2 (en) 1983-09-09 1986-03-20 C. Reichert Optische Werke Ag, Wien Device for avoiding splashing of liquid cooling media when quickly introducing biological-medical objects into a cooling bath as part of an immersion cryofixation

Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE3021747A1 (en) * 1980-06-10 1981-12-17 Siemens AG, 1000 Berlin und 8000 München Damaged pressure vessel removal system - comprises use of larger sealed transporting pressure vessel to completely enclose damaged vessel

Patent Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE3021747A1 (en) * 1980-06-10 1981-12-17 Siemens AG, 1000 Berlin und 8000 München Damaged pressure vessel removal system - comprises use of larger sealed transporting pressure vessel to completely enclose damaged vessel

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE3347803A1 (en) * 1982-09-17 1985-06-20 C. Reichert Optische Werke Ag, Wien Device for the disposal of media which are flammable or harmful to health, after use thereof as cooling medium, in an immersion cryofixing device
DE3332586C2 (en) 1983-09-09 1986-03-20 C. Reichert Optische Werke Ag, Wien Device for avoiding splashing of liquid cooling media when quickly introducing biological-medical objects into a cooling bath as part of an immersion cryofixation

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