DE3029865C2 - - Google Patents
Info
- Publication number
- DE3029865C2 DE3029865C2 DE19803029865 DE3029865A DE3029865C2 DE 3029865 C2 DE3029865 C2 DE 3029865C2 DE 19803029865 DE19803029865 DE 19803029865 DE 3029865 A DE3029865 A DE 3029865A DE 3029865 C2 DE3029865 C2 DE 3029865C2
- Authority
- DE
- Germany
- Prior art keywords
- stabilizer
- detection
- phosphoric acid
- impregnated
- ketones
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired
Links
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/64—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving ketones
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Molecular Biology (AREA)
- Biomedical Technology (AREA)
- Chemical & Material Sciences (AREA)
- Hematology (AREA)
- Immunology (AREA)
- Urology & Nephrology (AREA)
- Cell Biology (AREA)
- Microbiology (AREA)
- Biotechnology (AREA)
- Food Science & Technology (AREA)
- Medicinal Chemistry (AREA)
- Physics & Mathematics (AREA)
- Analytical Chemistry (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- General Physics & Mathematics (AREA)
- Pathology (AREA)
- Investigating Or Analyzing Non-Biological Materials By The Use Of Chemical Means (AREA)
- Investigating Or Analysing Biological Materials (AREA)
Description
Die Erfindung betrifft ein diagnostisches Mittel zum Nachweis von Ketonen in Flüssigkeiten, insbesondere Körperflüssigkeiten, beispielsweise von Acetessigsäure und Aceton im Urin, sowie ein Verfahren zu seiner Herstellung.The invention relates to a diagnostic agent for Detection of ketones in liquids, in particular Body fluids, such as acetoacetic acid and acetone in the urine, as well as a procedure its manufacture.
Für einen solchen Nachweis sind schon Teststreifen bekannt, die Natriumnitroprussid als Nachweissubstanz in Verbindung mit einem alkalischen Puffer sowie Stabilisatoren zur Verhinderung einer Zersetzung der Nachweissubstanz durch den alkalischen Puffer enthalten. Als Stabilisatoren enthalten diese bekannten Teststreifen organische filmbildende Substanzen (DE-PS 12 56 920) oder organische Säuren (DE-OS 27 33 426).Test strips are already used for such proof known, the sodium nitroprusside as a detection substance in combination with an alkaline buffer as well Stabilizers to prevent decomposition of the Detection substance contained by the alkaline buffer. These contain known stabilizers Test strips of organic film-forming substances (DE-PS 12 56 920) or organic acids (DE-OS 27 33 426).
Andererseits sind Teststreifen zum Nachweis von Blut und Urobilinogen in Körperflüssigkeiten bekannt, die als Stabilisator, für den Blutnachweis nach DE-PS 22 35 127 des Hydroperoxids, für den Urobilinogennachweis nach DE-OS 27 28 236 zur Erhöhung der Lagerbeständigkeit des fertigen Streifens, Phosphorsäuretrimorpholid enthalten. Der Mechanismus dieser Stabilisierungen ist nicht bekannt. Bekannt ist lediglich, daß eine Stabilisierung von Diazoniumsalzen, wie sie im Urobilinogentest verwendet werden, nicht erfolgt.On the other hand there are test strips for the detection of blood and urobilinogen in body fluids known to as a stabilizer, for blood detection according to DE-PS 22 35 127 of hydroperoxide, for urobilinogen detection according to DE-OS 27 28 236 to increase the shelf life of the finished strip, phosphoric acid trimorpholide contain. The mechanism of this stabilization is not known. It is only known that a stabilization of diazonium salts, like them used in the urobilinogen test, not done.
Der vorliegenden Erfindung liegt die Aufgabe zugrunde, einen weiteren hochwirksamen Stabilisator für Natriumnitroprussid zur Verfügung zu stellen.The present invention is based on the object another highly effective stabilizer for sodium nitroprusside to provide.
Diese Aufgabe wird durch das im Anspruch 1 gekennzeichnete Mittel sowie das im Anspruch 2 gekennzeichnete Verfahren gelöst.This object is characterized by that in claim 1 Means and the method characterized in claim 2 solved.
Es wurde gefunden, daß überraschenderweise das Phosphorsäuretrimorpholid auch in Teststreifen der oben beschriebenen Art für den Nachweis von Ketonen in Flüssigkeiten gute stabilisierende Wirkung hat.It has been found that, surprisingly, that Phosphoric acid trimorpholide also in test strips of Type described above for the detection of ketones has a good stabilizing effect in liquids.
Die folgenden Beispiele veranschaulichen die Verwendung des Phosphorsäuretrimorpholids als Stabilisator in Teststreifen für den Nachweis von Ketonen in Körperflüssigkeiten.The following examples illustrate its use of phosphoric acid trimorphide as a stabilizer in test strips for the detection of ketones in body fluids.
Papier 215 von Macherey-Nagel wird mit einer Lösung vonMacherey-Nagel paper 215 comes with a solution from
35 gGlycin 7,2 gNatronlauge in 100 mlWasser35 g glycine 7.2 g sodium hydroxide solution in 100 ml water
imprägniert und getrocknet.impregnated and dried.
Eine zweite Imprägnierung erfolgt mit einer Lösung vonA second impregnation is carried out with a solution from
0,5 gNatriumnitroprussid 0,5 gPhosphorsäuretrimorpholid 20 mlDimethylformamid 30 mlChloroform.0.5 g sodium nitroprusside 0.5 g phosphoric acid trimorpholide 20 ml dimethylformamide 30 ml chloroform.
Pufferimprägnierung wie in Beispiel 1; zweite Imprägnierung mit einer Lösung vonBuffer impregnation as in Example 1; second impregnation with a solution of
0,5 gNatriumnitroprussid 0,75 gPhosphorsäuretrimorpholid 30 mlDimethylformamid 20 mlChloroform.0.5 g sodium nitroprusside 0.75 g phosphoric acid trimorpholide 30 ml dimethylformamide 20 ml chloroform.
Claims (3)
Priority Applications (2)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
DE19803029865 DE3029865A1 (en) | 1980-08-07 | 1980-08-07 | Diagnostic agent for detecting ketone(s) in body liq. - comprises absorbent carrier impregnated with sodium nitro-cyanide, aminoacid, alkaline buffer and phosphoric acid tri:morpholide stabiliser |
DE20321161U DE20321161U1 (en) | 1980-08-07 | 2003-12-27 | Trailer for coupling to especially private motor vehicle has floor section with at least one lowerable section adjustable between upper travelling position for when trailer is on move, and lower position for stationary use of trailer |
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
DE19803029865 DE3029865A1 (en) | 1980-08-07 | 1980-08-07 | Diagnostic agent for detecting ketone(s) in body liq. - comprises absorbent carrier impregnated with sodium nitro-cyanide, aminoacid, alkaline buffer and phosphoric acid tri:morpholide stabiliser |
Publications (2)
Publication Number | Publication Date |
---|---|
DE3029865A1 DE3029865A1 (en) | 1982-05-19 |
DE3029865C2 true DE3029865C2 (en) | 1988-03-24 |
Family
ID=6109061
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
DE19803029865 Granted DE3029865A1 (en) | 1980-08-07 | 1980-08-07 | Diagnostic agent for detecting ketone(s) in body liq. - comprises absorbent carrier impregnated with sodium nitro-cyanide, aminoacid, alkaline buffer and phosphoric acid tri:morpholide stabiliser |
Country Status (1)
Country | Link |
---|---|
DE (1) | DE3029865A1 (en) |
Families Citing this family (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US4970172A (en) * | 1986-12-22 | 1990-11-13 | Abbott Laboratories | Method and device for ketone measurements |
US5071769A (en) * | 1986-12-22 | 1991-12-10 | Abbott Laboratories | Method and device for ketone measurement |
Family Cites Families (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
DE2728236B2 (en) * | 1977-06-23 | 1980-09-18 | Wolfgang Dr. 3300 Braunschweig Hirsch | Stabilized diagnostic preparation for the detection of urobilinogen |
DE2733426C3 (en) * | 1977-07-23 | 1981-08-06 | Behringwerke Ag, 3550 Marburg | Diagnostic agent for the detection of ketone bodies in liquids and process for its manufacture |
-
1980
- 1980-08-07 DE DE19803029865 patent/DE3029865A1/en active Granted
Also Published As
Publication number | Publication date |
---|---|
DE3029865A1 (en) | 1982-05-19 |
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Legal Events
Date | Code | Title | Description |
---|---|---|---|
8110 | Request for examination paragraph 44 | ||
D2 | Grant after examination | ||
8364 | No opposition during term of opposition | ||
8327 | Change in the person/name/address of the patent owner |
Owner name: MACHEREY, NAGEL & CO, 52355 DUEREN, DE |
|
8339 | Ceased/non-payment of the annual fee |