DE3029865C2 - - Google Patents

Info

Publication number
DE3029865C2
DE3029865C2 DE19803029865 DE3029865A DE3029865C2 DE 3029865 C2 DE3029865 C2 DE 3029865C2 DE 19803029865 DE19803029865 DE 19803029865 DE 3029865 A DE3029865 A DE 3029865A DE 3029865 C2 DE3029865 C2 DE 3029865C2
Authority
DE
Germany
Prior art keywords
stabilizer
detection
phosphoric acid
impregnated
ketones
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired
Application number
DE19803029865
Other languages
German (de)
Other versions
DE3029865A1 (en
Inventor
Karl-Heinz Dr. 5166 Kreuzau De Flegler
E. Dr. 5160 Dueren De Radmacher
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Macherey Nagel GmbH and Co KG
Original Assignee
Macherey Nagel & Co Chemikalienhandel 5160 Dueren De
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Macherey Nagel & Co Chemikalienhandel 5160 Dueren De filed Critical Macherey Nagel & Co Chemikalienhandel 5160 Dueren De
Priority to DE19803029865 priority Critical patent/DE3029865A1/en
Publication of DE3029865A1 publication Critical patent/DE3029865A1/en
Application granted granted Critical
Publication of DE3029865C2 publication Critical patent/DE3029865C2/de
Priority to DE20321161U priority patent/DE20321161U1/en
Granted legal-status Critical Current

Links

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/64—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving ketones

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Biomedical Technology (AREA)
  • Chemical & Material Sciences (AREA)
  • Hematology (AREA)
  • Immunology (AREA)
  • Urology & Nephrology (AREA)
  • Cell Biology (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Food Science & Technology (AREA)
  • Medicinal Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Analytical Chemistry (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • General Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Investigating Or Analyzing Non-Biological Materials By The Use Of Chemical Means (AREA)
  • Investigating Or Analysing Biological Materials (AREA)

Description

Die Erfindung betrifft ein diagnostisches Mittel zum Nachweis von Ketonen in Flüssigkeiten, insbesondere Körperflüssigkeiten, beispielsweise von Acetessigsäure und Aceton im Urin, sowie ein Verfahren zu seiner Herstellung.The invention relates to a diagnostic agent for Detection of ketones in liquids, in particular Body fluids, such as acetoacetic acid and acetone in the urine, as well as a procedure its manufacture.

Für einen solchen Nachweis sind schon Teststreifen bekannt, die Natriumnitroprussid als Nachweissubstanz in Verbindung mit einem alkalischen Puffer sowie Stabilisatoren zur Verhinderung einer Zersetzung der Nachweissubstanz durch den alkalischen Puffer enthalten. Als Stabilisatoren enthalten diese bekannten Teststreifen organische filmbildende Substanzen (DE-PS 12 56 920) oder organische Säuren (DE-OS 27 33 426).Test strips are already used for such proof known, the sodium nitroprusside as a detection substance in combination with an alkaline buffer as well Stabilizers to prevent decomposition of the Detection substance contained by the alkaline buffer. These contain known stabilizers Test strips of organic film-forming substances (DE-PS 12 56 920) or organic acids (DE-OS 27 33 426).

Andererseits sind Teststreifen zum Nachweis von Blut und Urobilinogen in Körperflüssigkeiten bekannt, die als Stabilisator, für den Blutnachweis nach DE-PS 22 35 127 des Hydroperoxids, für den Urobilinogennachweis nach DE-OS 27 28 236 zur Erhöhung der Lagerbeständigkeit des fertigen Streifens, Phosphorsäuretrimorpholid enthalten. Der Mechanismus dieser Stabilisierungen ist nicht bekannt. Bekannt ist lediglich, daß eine Stabilisierung von Diazoniumsalzen, wie sie im Urobilinogentest verwendet werden, nicht erfolgt.On the other hand there are test strips for the detection of blood and urobilinogen in body fluids known to as a stabilizer, for blood detection according to DE-PS 22 35 127 of hydroperoxide, for urobilinogen detection according to DE-OS 27 28 236 to increase the shelf life of the finished strip, phosphoric acid trimorpholide contain. The mechanism of this stabilization is not known. It is only known that a stabilization of diazonium salts, like them used in the urobilinogen test, not done.

Der vorliegenden Erfindung liegt die Aufgabe zugrunde, einen weiteren hochwirksamen Stabilisator für Natriumnitroprussid zur Verfügung zu stellen.The present invention is based on the object another highly effective stabilizer for sodium nitroprusside  to provide.

Diese Aufgabe wird durch das im Anspruch 1 gekennzeichnete Mittel sowie das im Anspruch 2 gekennzeichnete Verfahren gelöst.This object is characterized by that in claim 1 Means and the method characterized in claim 2 solved.

Es wurde gefunden, daß überraschenderweise das Phosphorsäuretrimorpholid auch in Teststreifen der oben beschriebenen Art für den Nachweis von Ketonen in Flüssigkeiten gute stabilisierende Wirkung hat.It has been found that, surprisingly, that Phosphoric acid trimorpholide also in test strips of Type described above for the detection of ketones has a good stabilizing effect in liquids.

Die folgenden Beispiele veranschaulichen die Verwendung des Phosphorsäuretrimorpholids als Stabilisator in Teststreifen für den Nachweis von Ketonen in Körperflüssigkeiten.The following examples illustrate its use of phosphoric acid trimorphide as a stabilizer in test strips for the detection of ketones in body fluids.

Beispiel 1example 1

Papier 215 von Macherey-Nagel wird mit einer Lösung vonMacherey-Nagel paper 215 comes with a solution from

35 gGlycin 7,2 gNatronlauge in 100 mlWasser35 g glycine 7.2 g sodium hydroxide solution in 100 ml water

imprägniert und getrocknet.impregnated and dried.

Eine zweite Imprägnierung erfolgt mit einer Lösung vonA second impregnation is carried out with a solution from

0,5 gNatriumnitroprussid 0,5 gPhosphorsäuretrimorpholid 20 mlDimethylformamid 30 mlChloroform.0.5 g sodium nitroprusside 0.5 g phosphoric acid trimorpholide 20 ml dimethylformamide  30 ml chloroform.

Beispiel 2Example 2

Pufferimprägnierung wie in Beispiel 1; zweite Imprägnierung mit einer Lösung vonBuffer impregnation as in Example 1; second impregnation with a solution of

0,5 gNatriumnitroprussid 0,75 gPhosphorsäuretrimorpholid 30 mlDimethylformamid 20 mlChloroform.0.5 g sodium nitroprusside 0.75 g phosphoric acid trimorpholide 30 ml dimethylformamide 20 ml chloroform.

Claims (3)

1. Diagnostisches Mittel zum Nachweis von Ketonen in Flüssigkeiten, bestehend aus einem saugfähigen Träger, der mit Natriumnitroprussid, einer wasserlöslichen Aminosäure, einer alkalischen Puffersubstanz und einem Stabilisator imprägniert ist, dadurch gekennzeichnet, daß als Stabilisator Phosphorsäuretrimorpholid eingesetzt wird.1. Diagnostic agent for the detection of ketones in liquids, consisting of an absorbent carrier, which is impregnated with sodium nitroprusside, a water-soluble amino acid, an alkaline buffer substance and a stabilizer, characterized in that phosphoric acid trimorpholide is used as the stabilizer. 2. Verfahren zur Herstellung eines diagnostischen Mittels zum Nachweis von Ketonen in Flüssigkeiten nach Anspruch 1, bei dem der saugfähige Träger in einem ersten Schritt mit der Aminosäure dem alkalischen Puffer in wäßriger Lösung imprägniert und getrocknet wird und dann in einem zweiten Schritt mit einer Lösung des Natriumnitroprussids in einem organischen Lösungsmittel bzw. Lösungsmittelgemisch, enthaltend einen Stabilisator, imprägniert und erneut getrocknet wird, dadurch gekennzeichnet, daß als Stabilisator Phosphorsäuretrimorpholid verwendet wird.2. Method of making a diagnostic For the detection of ketones in liquids according to claim 1, wherein the absorbent carrier in a first step with the amino acid the alkaline Impregnated buffer in aqueous solution and is dried and then in a second step a solution of sodium nitroprusside in one organic solvents or solvent mixtures, containing a stabilizer, impregnated and again is dried, characterized in that as Stabilizer phosphoric acid trimorpholide is used. 3. Verfahren nach Anspruch 2, dadurch gekennzeichnet, daß als Lösungsmittel für die zweite Imprägnierung ein Gemisch von Dimethylformamid und Chloroform verwendet wird.3. The method according to claim 2, characterized in that as a solvent for the second impregnation a mixture of dimethylformamide and chloroform was used becomes.
DE19803029865 1980-08-07 1980-08-07 Diagnostic agent for detecting ketone(s) in body liq. - comprises absorbent carrier impregnated with sodium nitro-cyanide, aminoacid, alkaline buffer and phosphoric acid tri:morpholide stabiliser Granted DE3029865A1 (en)

Priority Applications (2)

Application Number Priority Date Filing Date Title
DE19803029865 DE3029865A1 (en) 1980-08-07 1980-08-07 Diagnostic agent for detecting ketone(s) in body liq. - comprises absorbent carrier impregnated with sodium nitro-cyanide, aminoacid, alkaline buffer and phosphoric acid tri:morpholide stabiliser
DE20321161U DE20321161U1 (en) 1980-08-07 2003-12-27 Trailer for coupling to especially private motor vehicle has floor section with at least one lowerable section adjustable between upper travelling position for when trailer is on move, and lower position for stationary use of trailer

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
DE19803029865 DE3029865A1 (en) 1980-08-07 1980-08-07 Diagnostic agent for detecting ketone(s) in body liq. - comprises absorbent carrier impregnated with sodium nitro-cyanide, aminoacid, alkaline buffer and phosphoric acid tri:morpholide stabiliser

Publications (2)

Publication Number Publication Date
DE3029865A1 DE3029865A1 (en) 1982-05-19
DE3029865C2 true DE3029865C2 (en) 1988-03-24

Family

ID=6109061

Family Applications (1)

Application Number Title Priority Date Filing Date
DE19803029865 Granted DE3029865A1 (en) 1980-08-07 1980-08-07 Diagnostic agent for detecting ketone(s) in body liq. - comprises absorbent carrier impregnated with sodium nitro-cyanide, aminoacid, alkaline buffer and phosphoric acid tri:morpholide stabiliser

Country Status (1)

Country Link
DE (1) DE3029865A1 (en)

Families Citing this family (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4970172A (en) * 1986-12-22 1990-11-13 Abbott Laboratories Method and device for ketone measurements
US5071769A (en) * 1986-12-22 1991-12-10 Abbott Laboratories Method and device for ketone measurement

Family Cites Families (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE2728236B2 (en) * 1977-06-23 1980-09-18 Wolfgang Dr. 3300 Braunschweig Hirsch Stabilized diagnostic preparation for the detection of urobilinogen
DE2733426C3 (en) * 1977-07-23 1981-08-06 Behringwerke Ag, 3550 Marburg Diagnostic agent for the detection of ketone bodies in liquids and process for its manufacture

Also Published As

Publication number Publication date
DE3029865A1 (en) 1982-05-19

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Legal Events

Date Code Title Description
8110 Request for examination paragraph 44
D2 Grant after examination
8364 No opposition during term of opposition
8327 Change in the person/name/address of the patent owner

Owner name: MACHEREY, NAGEL & CO, 52355 DUEREN, DE

8339 Ceased/non-payment of the annual fee