DE2963T1 - Aqueous, stabilized fluorescent markings, proteins marked therewith and methods of application - Google Patents

Aqueous, stabilized fluorescent markings, proteins marked therewith and methods of application

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Publication number
DE2963T1
DE2963T1 DE1978300913 DE78300913T DE2963T1 DE 2963 T1 DE2963 T1 DE 2963T1 DE 1978300913 DE1978300913 DE 1978300913 DE 78300913 T DE78300913 T DE 78300913T DE 2963 T1 DE2963 T1 DE 2963T1
Authority
DE
Germany
Prior art keywords
styrene
fluorescent
acrylamido
poly
aqueous
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Pending
Application number
DE1978300913
Other languages
German (de)
Inventor
David Stanley Rochester Sundberg Michael William Penfield NY Frank (V St A )
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Eastman Kodak Co
Original Assignee
Eastman Kodak Co
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Eastman Kodak Co filed Critical Eastman Kodak Co
Publication of DE2963T1 publication Critical patent/DE2963T1/en
Pending legal-status Critical Current

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Claims (12)

ΕΡΛ-Anmeldung 78 3ÜO 913.7 DEUTSCHE OBERSETZUMG PEU ANSPRÜCHEΕΡΛ registration 78 3ÜO 913.7 GERMAN OBERSETZUMG PEU CLAIMS 1. .'/äßriges, stabilisiertes, fluoreszierendes .·'nrkierungsinittel, bestehend aus einem in Polymer-Latexteilchen eines beladbaren Polymer-Latex eingebauten fluoreszierenden Seltenen-Erd-Chelat.1.. '/ Aqueous, stabilized, fluorescent. consisting of a fluorescent built into polymer latex particles of a loadable polymer latex Rare earth chelate. 2. Uarkierungsmittel nach Anspruch 1, in dem das fluoreszierende Seltene-Erd-Chelat bis zu 7,5 Gev/.-'o der Teilchen ausmacht.2. Uarkierungsmittel according to claim 1, in which the fluorescent Rare earth chelate up to 7.5 Gev /.- 'o of the particles. 3. Markierungsmittel nach Anspruch 1 oder Anspruch 2, in dem das Seltene-Erd-Chelat ein Chelat des Europiums oder Terbiums ist.3. Marking agent according to claim 1 or claim 2, in which the rare earth chelate is a chelate of europium or terbium is. 4. Markierungsmittel nach Ansprüchen 1, 2 oder 3, in dem die Polymer-Latexteilchen enthalten oder bestehen aus einer4. Marking means according to claims 1, 2 or 3, in which the polymer latex particles contain or consist of one (1) Poly(n-butylacrylat-co-styrol-co-2-acrylamido-2-methylpropansulphonsäure , (2) Poly(n-butylacrylat-co-2-acrylamido-2-methylpropansulphonsäure, (3) Poly/ styrol-co- (2-chloräthylsulphonylmethyl)styrol-co-2-acrylamido-2-methyIpropansulphonsäure_7, (4) Poly/ styrol-co-acrylamido-co- (2-chloräthylsulphonylmethyl)styrol-co-2-acrylamido-2-methylpropansulphonsäure, (5) Poly(n-butyIacrylat-co-styro1-co-m+p-chlorine thy Is tyro I- co- 2 -acrylamido -2 -methylpropansulphonsäure , (6) Poly/ n-butylacrylat-co-styrol-co-m+p-chlormethylstyro1-co-2-(methacryloyloxy)äthyltrimethylammoniummethosulphat_7 oder (7) Poly/ styrol-co-acrylamido-co-(2-chloräthylsulphonylmethyl)styrol J. (1) poly (n-butyl acrylate-co-styrene-co-2-acrylamido-2-methylpropane sulphonic acid, (2) poly (n-butyl acrylate-co-2-acrylamido-2-methylpropane sulphonic acid, (3) poly / styrene-co - (2-chloroethylsulphonylmethyl) styrene-co-2-acrylamido-2-methyIpropansulphonsäure_7, (4) poly / styrene-co-acrylamido-co- (2-chloroethylsulphonylmethyl) styrene-co-2-acrylamido-2-methylpropane sulphonic acid, (5 ) Poly (n-butyl acrylate-co-styro1-co-m + p-chlorine thy is tyro I- co- 2 -acrylamido -2 -methylpropanesulphonic acid, (6) poly / n-butyl acrylate-co-styrene-co-m + p-chloromethylstyro1-co-2- (methacryloyloxy) äthyltrimethylammoniummethosulphat_7 or (7) poly / styrene-co-acrylamido-co- (2-chloroethylsulphonylmethyl) styrene J. 5. Markierungsmittel nach einem der Ansprüche 1 bis 4, in dem das fluoreszierende Seltene-Erd-Chelat aus einer Seltenen Erde und einem Chelatbildner aus einem 1,3-Diketon, Phthalat, Naphthalat, Di-pyridin, Terpyridin, ρ-Benzoylbenzoat, p-Benzoylacetonat oder Phenanthralin gebildet ist.5. A marker according to any one of claims 1 to 4, wherein the fluorescent rare earth chelate is from a rare earth and a chelating agent from a 1,3-diketone, phthalate, Naphthalate, di-pyridine, terpyridine, ρ-benzoyl benzoate, p-benzoylacetonate or phenanthralin is formed. 030552/0069030552/0069 0QQ29B30QQ29B3 6. Fluoreszierend markiertes Protein aus einem Protein, das an ein wäßriges stabilisiertes, fluoreszierendes Markierungsmittel gemäß einem der Ansprüche 1 bis 5 adsorbiert oder kovalent gebunden ist.6. Fluorescently labeled protein from a protein that is an aqueous stabilized fluorescent marker according to any one of claims 1 to 5 is adsorbed or covalently bound. 7. Markiertes Protein gemäß Anspruch 6, in dem das Protein ein Antikörper, Antigen oder Enzym ist.7. Labeled protein according to claim 6, in which the protein is an antibody, antigen or enzyme. 8. Fluoreszierend markiertes Immunoreagens mit oder aus einem Antigen oder Antikörper, das bzw. der an ein wäßriges stabilisiertes fluoreszierendes Markierungsmittel gemäß einem der Ansprüche 1 bis 5 adsorbiert oder kovalent gebunden ist.8. Fluorescently labeled immunoreagent with or from a Antigen or antibody linked to an aqueous stabilized fluorescent marker according to one of claims 1 to 5 is adsorbed or covalently bound. 9. Verfahren zur Herstellung eines wäßrigen, stabilisierten fluoreszierenden Ilarkierungsmittels gemäß Ansprüchen 1 bis 5, bei dem man eine Lösung eines fluoreszierenden Seltenen-Erd-Chelates in einem nit Wasser mischbaren Lösungsmittel erzeugt und daraufhin mit einein beladbaren Polymer-Latex vermischt, wodurch das Seltene-Erd-Chelat in die hydrophoben Teilchen des Latex eingebaut wird.9. A process for the preparation of an aqueous, stabilized fluorescent Ilarkierungsmittel according to claims 1 to 5, using a solution of a fluorescent rare earth chelate generated in a water-miscible solvent and then mixed with a loadable polymer latex, thereby transforming the rare earth chelate into the hydrophobic particles of the latex is incorporated. 10. Verfahren zur Herstellung eines markierten Immunoreagens gemäß Anspruch 8, bei dem ein wäßriges stabilisiertes, fluoreszierendes Ilarkierungsmittel gemäß einem der Ansprüche 1 bis 5 mit einer wäßrigen Lösung eines Antigens oder Antikörpers vermischt wird.10. A method for the preparation of a labeled immunoreagent according to claim 8, in which an aqueous stabilized, fluorescent marking agent according to any one of the claims 1 to 5 is mixed with an aqueous solution of an antigen or antibody. 11. Verfahren zur Durchführung einer Immunobestimmung unter Verwendung eines maikierten Immunoreagens gemäß Anspruch 8, bei dem nach Vermischen mit einer Testprobe die Fluoreszenz der überstehenden Flüssigkeit oder irgendein Niederschlag gemessen wird.11. Method of Performing an Immuno-Determination Using of a maiked immunoreagent according to claim 8, in which, after mixing with a test sample, the fluorescence of the supernatant liquid or any precipitate is measured. 030552/0069030552/0069 12. Verfahren nach Anspruch 11, bei dem die Fluoreszenz unter Arwendung einer zeitlichen Auflösungstechnik gemessen vird.12. The method of claim 11, wherein the fluorescence is below Using a temporal resolution technique. 030 5 52/0069030 5 52/0069
DE1978300913 1977-12-28 1978-12-28 Aqueous, stabilized fluorescent markings, proteins marked therewith and methods of application Pending DE2963T1 (en)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
US86527477 1977-12-28

Publications (1)

Publication Number Publication Date
DE2963T1 true DE2963T1 (en) 1980-06-26

Family

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