DE2841455A1 - Electrodic method for metabolic stimulation of biological cells - by applying alternating electric fields to two electrodes in culture vessel contg. cells - Google Patents

Electrodic method for metabolic stimulation of biological cells - by applying alternating electric fields to two electrodes in culture vessel contg. cells

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Publication number
DE2841455A1
DE2841455A1 DE19782841455 DE2841455A DE2841455A1 DE 2841455 A1 DE2841455 A1 DE 2841455A1 DE 19782841455 DE19782841455 DE 19782841455 DE 2841455 A DE2841455 A DE 2841455A DE 2841455 A1 DE2841455 A1 DE 2841455A1
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Germany
Prior art keywords
cells
culture vessel
electrodes
alternating
electric fields
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Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Withdrawn
Application number
DE19782841455
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German (de)
Inventor
Dieter Dr Ing Gerth
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
KM Kabelmetal AG
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KM Kabelmetal AG
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Application filed by KM Kabelmetal AG filed Critical KM Kabelmetal AG
Priority to DE19782841455 priority Critical patent/DE2841455A1/en
Publication of DE2841455A1 publication Critical patent/DE2841455A1/en
Withdrawn legal-status Critical Current

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N13/00—Treatment of microorganisms or enzymes with electrical or wave energy, e.g. magnetism, sonic waves

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  • Zoology (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Genetics & Genomics (AREA)
  • Chemical & Material Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Biomedical Technology (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Biotechnology (AREA)
  • Immobilizing And Processing Of Enzymes And Microorganisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)

Abstract

Induction and stimulation of growth, proliferation and other metabolic functions of plant, animal and bacterial cells comprises subjecting the cells to alternating electrical fields under sterile conditions in a suitable nutrient medium. In a new appts. for carrying out the above process, two electrodes are inserted into the culture vessel contg. the cells, suitably arranged for application of the alternating field. Alternating fields stimulate cell growth and proliferation, so that costly fermentation and cell culture processes proceed more rapidly and require smaller amounts of nutrient medium. The process enables substrates to be used that normally are not utilised by the cells concerned.

Description

Verfahren zur Induktion und Stimulation des Wachstums, der VermehrungProcess for the induction and stimulation of growth, multiplication

und anderer Stoffwechsel leistungen von Zellen Die vorliegende Erfindung betrifft ein Verfahren zur Induktion und Stimulation des Wachstums, der Vermehrung und anderer Stoffwechselleistungen von pflanzlichen, tierischen oder bakteriellen Zellen.and other metabolic functions of cells The present invention relates to a method for the induction and stimulation of growth, reproduction and other metabolic functions of vegetable, animal or bacterial Cells.

Zellen werden heutzutage in großem Maßstab und aus vielerlei Gründen fermentiert. Da die zum "Wachstum" benötigten Kulturmedien oft sehr kostspielig sind und eine Fermentation mit hohen Betriebskosten verbunden ist, sind alle Wege, die, ohne große zusätzliche Kosten, eine solche Fermentation verkürzen, von großem Interesse.Cells are being made on a large scale these days for a variety of reasons fermented. Since the culture media required for "growth" are often very expensive and fermentation is associated with high operating costs, are all ways which, at no great additional cost, shorten such a fermentation, by a great deal Interest.

Der Erfindung liegt von daher die Aufgabe zugrunde, eine Möglichkeit zu finden, auf kostengünstigem Wege das Wachstum, die Vermehrung und andere Stoffwechsel leistungen von pflanzlichen, tierischen oder bakteriellen Zellen zu initiieren oder zu stimulieren.The invention is therefore based on the object of one possibility to find, in an inexpensive way, growth, reproduction and other metabolism to initiate the services of plant, animal or bacterial cells or to stimulate.

Diese Aufgabe wird gemäß der Erfindung dadurch gelöst, daß die Zellen in einem für sie geeigneten Nährstoffmedium unter sterilen Bedingungen elektrischen Wechselfeldern ausgesetzt werden. Durch Anwendung von Feldern in der Größenordnung von Bruchteilen von Hz bis zur Größenordnung technischer Wechsel ströme wird das Wachstum tierischer, pflanzlicher sowie bakterieller Zellen über das bekannte Maß hinaus erheblich gefördert.This object is achieved according to the invention in that the cells in a suitable nutrient medium under sterile conditions Are exposed to alternating fields. Using fields in the The order of magnitude of fractions of Hz up to the order of magnitude of technical alternating currents the growth of animal, plant and bacterial cells is beyond the known Extent also promoted considerably.

Kostspielige Fermentationsprozesse können durch die Erfindung erheblich verkürzt und gleichzeitig die Wahrscheinlichkeit von Verlusten der Ansätze durch Infektion mit unerwünschten Organismen auf ein Minimum herabgesetzt werden.Expensive fermentation processes can be significantly reduced by the invention shortened and at the same time the probability of losses of the approaches through Infection with unwanted organisms is reduced to a minimum.

In Weiterbildung der Erfindung kann das erfindungsgemäße Verfahren auch dafür benutzt werden, Metaboliten mit antibakterieller, antifungischer, antiviraler, antitumoraler und pharmakologischer Wirkung oder mit einer oder mehrerer dieser Eigenschaften, bzw. von Enzymen, in kürzerer Zeit zu gewinnen. Es besteht darüber hinaus die Möglichkeit, daß durch die Wechselwirkungen zwischen Zellen und angelegtem elektrischen Feld nur das Wachstum oder die Vermehrung stimuliert wird, sondern auch andere Fähigkeiten der Zelle induziert und/oder verstärkt werden Eine weitere vorteilhafte Möglichkeit ist in Anwendung der Erfindung die, den Zellen Substrate zuzuführen, die sie sonst aufzunehmen nicht in der Lage sind.In a further development of the invention, the method according to the invention can also be used to treat metabolites with antibacterial, antifungal, antiviral, anti-tumor and pharmacological effect or with one or more of these Properties, or of enzymes, to be gained in a shorter time. It insists on it in addition, the possibility that through the interactions between cells and applied electric field only stimulates growth or reproduction, but rather other abilities of the cell can also be induced and / or strengthened An advantageous possibility in application of the invention is that the cells are substrates that they would otherwise not be able to absorb.

Zur Durchführung des Verfahrens nach der Erfindung hat sich der Einsatz breitbandiger Frequenzgeneratoren mit ausreichend hohem Innenwiderstand als zweckmäßig erwiesen. Die hiermit erzeugten Wechselfelder können dann impulsweise und/oder auch mit zeitlicher Unterbrechung angelegt werden Zu diesem Zweck wird man die Anordnung in Weiterführung des Erfindungsgedankens vorteilhaft so treffen, daß in ein die Zellen enthaltendes Kulturgefäß mit Nährlösung zwei sterilisierbare Elektroden in geeigneter Zuordnung eingeführt und mit den elektrischen Wechselfeldern gewünschter Frequenz beaufschlagt werden. Von den Elektroden kann hierbei z B. die eine ring- oder plattenförmig ausgebildet und am Boden des Kulturgefäßes angeordnet sein, während die zweite Elektrode stabförmig ausgebildet ist und von oben in das Kulturgefäß hineinragt. Wesentlich ist ferner, daß die für das Verfahren nach der Erfindung verwendeten Elektroden chemisch inert, ohne Katalysatorwirkung, nicht toxisch für die jeweiligen Zellen und sterilisierbar sind.To carry out the method according to the invention, the use broadband frequency generators with a sufficiently high internal resistance as appropriate proven. The alternating fields generated in this way can then be pulsed and / or also to be created with a time interruption. For this purpose one becomes the arrangement in a continuation of the inventive concept advantageously meet so that in a Culture vessel containing cells with nutrient solution and two sterilizable electrodes in appropriate assignment introduced and desired with the alternating electric fields Frequency are applied. Of the electrodes, for example, one ring- or be plate-shaped and arranged at the bottom of the culture vessel, while the second electrode is rod-shaped and from above into the culture vessel protrudes. It is also essential that the for the method according to the invention Electrodes used are chemically inert, without catalytic effect, non-toxic for the respective cells and can be sterilized.

Claims (8)

Patentansprüche 1. Verfahren zur Induktion und Stimulation des Wachstums, der Vermehrung und anderer Stoffwechsel leistungen von pflanzlichen, tierischen oder bakteriellen Zellen, dadurch gekennzeichnet, daß die Zellen in einem für sie geeigneten Nährstoffmedium unter sterilen Bedingungen elektrischen Wechselfeldern ausgesetzt werden. Claims 1. Method for inducing and stimulating growth, the reproduction and other metabolic functions of plant and animal species or bacterial cells, characterized in that the cells in one for them suitable nutrient medium under sterile conditions with alternating electric fields get abandoned. 2. Verfahren nach Anspruch 1, gekennzeichnet durch seine Anwendung zur beschleunigten Produktion und Erhöhung der Syntheserate von Metaboliten mit antibakterieller, antifungischer, antiviraler, antitumoraler und pharmakologischer Wirkung oder mehreren dieser Eigenschaften, bzw. von Enzymen.2. The method according to claim 1, characterized by its application to accelerate the production and increase of the synthesis rate of metabolites with antibacterial, antifungal, antiviral, antitumoral and pharmacological Effect or several of these properties or of enzymes. 3. Verfahren nach Anspruch 1, gekennzeichnet durch seine Anwendung zur Einschleusung von sonst nicht verwertbaren Substraten in die Zellen.3. The method according to claim 1, characterized by its application for the smuggling of otherwise unusable substrates into the cells. 4. Verfahren nach Anspruch 1 oder einem folgenden, dadurch gekennzeichnet, daß das Wechselfeld impulsweise angelegt wird.4. The method according to claim 1 or one of the following, characterized in that that the alternating field is applied in pulses. 5. Verfahren nach Anspruch 1 oder einem folgenden, dadurch gekennzeichnet, daß das Wechselfeld mit zeitlicher Unterbrechung angelegt wird.5. The method according to claim 1 or one of the following, characterized in that that the alternating field is applied with a temporal interruption. 6. Vorrichtung zur Durchführung des Verfahrens nach Anspruch 1 oder einem der folgenden, dadurch gekennzeichnet, daß in ein die Zellen enthalten- des Kulturgefäß zwei Elektroden zum Anlegen des Wechselfeldes in geeigneter Zuordnung eingeführt sind.6. Apparatus for performing the method according to claim 1 or one of the following, characterized in that the cells contained in a of Culture vessel with two electrodes for applying the alternating field in a suitable allocation are introduced. 7. Vorrichtung nach Anspruch 6, dadurch gekennzeichnet, daß die eine Elektrode ring- oder plattenförmig ausgebildet und am Boden des Kulturgefäßes angeordnet ist, während die zweite Elektrode stabförmig von oben in das Kulturgefäß hineinragt.7. Apparatus according to claim 6, characterized in that the one Electrode designed in the shape of a ring or plate and arranged at the bottom of the culture vessel while the second electrode protrudes in the shape of a rod from above into the culture vessel. 8. Vorrichtung nach Anspruch 6 oder 7, dadurch gekennzeichnet, daß die verwendeten Elektroden chemisch inert, ohne Katalysatorwirkung, nicht toxisch für die jeweiligen Zellen und sterilisierbar sind.8. Apparatus according to claim 6 or 7, characterized in that the electrodes used are chemically inert, without catalytic effect, non-toxic for the respective cells and can be sterilized.
DE19782841455 1978-09-23 1978-09-23 Electrodic method for metabolic stimulation of biological cells - by applying alternating electric fields to two electrodes in culture vessel contg. cells Withdrawn DE2841455A1 (en)

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DE19782841455 DE2841455A1 (en) 1978-09-23 1978-09-23 Electrodic method for metabolic stimulation of biological cells - by applying alternating electric fields to two electrodes in culture vessel contg. cells

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DE19782841455 DE2841455A1 (en) 1978-09-23 1978-09-23 Electrodic method for metabolic stimulation of biological cells - by applying alternating electric fields to two electrodes in culture vessel contg. cells

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DE2841455A1 true DE2841455A1 (en) 1980-04-10

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Cited By (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4487834A (en) * 1982-09-17 1984-12-11 Biolectron, Inc. Electrical stimulation of articular chondrocytes
FR2554827A1 (en) * 1983-11-11 1985-05-17 Shinryo Corp METHOD FOR ACTIVATING THE BIOREACTIVITY OF MICROORGANISMS
WO1987002705A1 (en) * 1985-10-29 1987-05-07 Sweeney George William Jr Method for accelerating growth rates
EP0223110A1 (en) * 1985-10-26 1987-05-27 Heinz Doevenspeck Treatment of microorganisms promoting their metabolism and/or growth, and apparatus for carrying out the method

Cited By (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4487834A (en) * 1982-09-17 1984-12-11 Biolectron, Inc. Electrical stimulation of articular chondrocytes
FR2554827A1 (en) * 1983-11-11 1985-05-17 Shinryo Corp METHOD FOR ACTIVATING THE BIOREACTIVITY OF MICROORGANISMS
EP0223110A1 (en) * 1985-10-26 1987-05-27 Heinz Doevenspeck Treatment of microorganisms promoting their metabolism and/or growth, and apparatus for carrying out the method
WO1987002705A1 (en) * 1985-10-29 1987-05-07 Sweeney George William Jr Method for accelerating growth rates

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