DE2640892C2 - Gel bed electrophoretic analysis set-up - Google Patents

Description

The present invention relates to an electrophoretic gel bed analysis device according to the preamble of claim 1.

Such an analysis arrangement is known from US Pat. No. 3,719,580. The two buffer chambers of this known analysis arrangements have the shape of flat, rectangular cuvettes and the electrodes consist of Wires stretched between the inner walls of the narrow sides of the cuvettes and over a connecting conductor are connected to a clamp arranged at the upper, open end of the relevant cuvette. Between The two cuvettes have a U-shaped seal and the arrangement of the cuvettes and the The seal is held together by spring straps attached to the outside of the narrow sides of the Cuvettes are attached.

From US-PS 35 06 554 an electrophoretic gel bed analysis arrangement is known in which the separation gel is arranged in a vertical tube. At the bottom the tube ends at a distance from the bottom of one shell-like structure, which is filled with a gel plug and in turn in a buffer solution filled chamber is located. The upper end of the tube protrudes from a side hole in the tube wall with a second buffer chamber in connection.

It is an object of the present invention to provide an electrophoretic gel bed analysis arrangement of the type mentioned in such a way that it is simple in structure, easily disassembled, Can be cleaned and reassembled and performed quickly Analyzes made possible.

According to the invention, this object is achieved by an electrophoretic gel bed analysis arrangement of the initially introduced mentioned type with the characterizing feature of claim 1 solved.

A further development of the present gel bed analysis arrangement is the subject of the dependent claim.

The drawing shows a simplified representation of an embodiment of the gel bed analysis chamber arrangement according to the invention.

The illustrated embodiment of the invention contains two cuvette-like buffer chambers 10 and 12 and a spacer 14, which is used to delimit and seal a separation chamber, which when mounted Gel bed analysis chamber arrangement by two flat walls 10a lying parallel and opposite one another and 12a of the buffer chamber 10 or 12 and the spacer 14 tightly clamped between them will.

The wall 10 a of the buffer chamber 10 has a slot-shaped opening 16 below through which the interior of the Buffer chamber 10 with the analysis chamber arrangement mounted with the lower end of the separation chamber in Connection. The wall 12a of the buffer chamber 12 has a cutout 18 at the top, which acts as an overflow and When the analysis chamber arrangement is mounted, the interior of the buffer chamber 12 with the upper end of the Separation chamber connects the between the slot-shaped opening 16 and the lower edge of the Part of the separating chamber located in the cutout 18 forms the actual separating distance.

The buffer chambers can be made of glass or plastic, while the spacer 14 is preferably made of an elastic material, such as silicone rubber, and with inserts 14a made of hard material is provided which has a defined distance between the walls 10a, 12a delimiting the separation chamber guaranteed. A given separation chamber thickness can also be maintained in other ways, z. B. by appropriately dimensionally accurate training of the bracket, through which the buffer chambers and the Spacers are held together in operation.

To hold and clamp the buffer chambers 10, 12 and the spacer 14 can a U-shaped holder 20 or some other clamping connection or bracket are used.

A cover 22, which is made of insulating material, serves to terminate the separating chamber arrangement at the top hold two electrodes 26, 28, which extend into the buffer chamber 10 and 12 with the lid on.

To carry out an analysis, the two buffer chambers 10 and 12 and the spacer 14 clamped in the holder 20 with a gel bed in the space enclosed by the spacer. In the Buffer chamber 10, a gel plug 30 is introduced, which with the lower end of the gel strip in the Separation chamber through the slot-shaped opening 16 is in communication. The remaining space Above the gel plug 30 is filled with buffer solution. The buffer chamber 12 is so far with Buffer solution filled until it flows over the lower edge of the cut-out and with the gel strip in the Separation chamber comes into contact.

Now the cover 22 with the electrodes 26 and 28 is put on, the electrodes are attached to a suitable one Power source is connected and the analysis is carried out in the usual way.

The embodiment described can be modified in the most varied of ways. For example can instead of the common cover 22, a separate cover can also be used for each buffer chamber then need not consist of insulating material.

1 sheet of drawings

Claims (2)

Patent claims: 1. Electrophoretic gel bed analysis arrangement with a separation chamber for receiving an im essentially vertically arranged, flat gel strips and two on both sides of the separation chamber arranged buffer chambers, each containing an electrode and with a side wall the separation chamber adjoin, and one of which via an upper opening and the second via ι ο a lower slot-shaped opening provided in the relevant side wall, each with one of two areas of the separating chamber separated by a separating distance and delimiting this are in communication, characterized in that the second buffer chamber (10) to is filled via the slot-shaped opening (16) with a gel plug (30) which closes it and that the electrodes (26, 28) held by at least one cover (22) for the buffer chambers (10, 12) are. 2. Gel bed analysis arrangement according to claim 1, in which the buffer chambers have the shape of shallower, rectangular cuvettes have, in conjunction with a substantially U-shaped seal the Bounding the separating chamber, characterized by a holder (20) for insertion and clamping the buffer chambers (10, 12) with the one between them, as a spacer formed U-shaped seal (14).