DE262432C - - Google Patents
Info
- Publication number
- DE262432C DE262432C DENDAT262432D DE262432DA DE262432C DE 262432 C DE262432 C DE 262432C DE NDAT262432 D DENDAT262432 D DE NDAT262432D DE 262432D A DE262432D A DE 262432DA DE 262432 C DE262432 C DE 262432C
- Authority
- DE
- Germany
- Prior art keywords
- filter
- blue violet
- glass
- violet glass
- quartz
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Active
Links
- 239000011521 glass Substances 0.000 claims description 13
- 240000000358 Viola adunca Species 0.000 claims description 10
- 235000005811 Viola adunca Nutrition 0.000 claims description 10
- 235000013487 Viola odorata Nutrition 0.000 claims description 10
- 235000002254 Viola papilionacea Nutrition 0.000 claims description 10
- 229910052904 quartz Inorganic materials 0.000 claims description 6
- 239000010453 quartz Substances 0.000 claims description 6
- VYPSYNLAJGMNEJ-UHFFFAOYSA-N silicon dioxide Inorganic materials O=[Si]=O VYPSYNLAJGMNEJ-UHFFFAOYSA-N 0.000 claims description 6
- 239000007788 liquid Substances 0.000 claims description 4
- 239000000463 material Substances 0.000 claims description 2
- 230000000875 corresponding Effects 0.000 claims 1
- ARUVKPQLZAKDPS-UHFFFAOYSA-L Copper(II) sulfate Chemical compound [Cu+2].[O-][S+2]([O-])([O-])[O-] ARUVKPQLZAKDPS-UHFFFAOYSA-L 0.000 description 2
- 239000002023 wood Substances 0.000 description 2
- 241000894006 Bacteria Species 0.000 description 1
- LGDPTPLJZGPOJL-UHFFFAOYSA-N N,N-dimethyl-2-nitrosoaniline Chemical compound CN(C)C1=CC=CC=C1N=O LGDPTPLJZGPOJL-UHFFFAOYSA-N 0.000 description 1
- 238000010521 absorption reaction Methods 0.000 description 1
- 239000004568 cement Substances 0.000 description 1
- 238000001816 cooling Methods 0.000 description 1
- 229910000365 copper sulfate Inorganic materials 0.000 description 1
- 239000012530 fluid Substances 0.000 description 1
- 238000005286 illumination Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 238000006011 modification reaction Methods 0.000 description 1
- 239000002245 particle Substances 0.000 description 1
Classifications
-
- G—PHYSICS
- G02—OPTICS
- G02B—OPTICAL ELEMENTS, SYSTEMS OR APPARATUS
- G02B21/00—Microscopes
- G02B21/0004—Microscopes specially adapted for specific applications
Landscapes
- Physics & Mathematics (AREA)
- Chemical & Material Sciences (AREA)
- Analytical Chemistry (AREA)
- General Physics & Mathematics (AREA)
- Optics & Photonics (AREA)
- Microscoopes, Condenser (AREA)
Description
KAISERLICHESIMPERIAL
PATENTAMT.PATENT OFFICE.
Die Erfindung betrifft Filter für Fluoreszenzmikroskope , welche nur ultraviolettes Licht hindurchlassen, wie sie z. B. von Wood angegeben worden sind.The invention relates to filters for fluorescence microscopes, which only ultraviolet Let light through as it is z. B. indicated by Wood.
Durch das deutsche Gebrauchsmuster 443863 ist eine Abänderung und Verbesserung des Filters nach Wood bekannt geworden. Bei diesem Filter sind zwei flüssige Filterkömponenten (Lösungen von Nitrosodimethylanilin und Kupfersulfat) zwischen Wänden aus Jenenser Blauuviolglas angeordnet.The German utility model 443863 is a modification and improvement of the Filters after Wood became known. There are two liquid filter components in this filter (Solutions of nitrosodimethylaniline and copper sulfate) arranged between walls made of Jenenser blue violet glass.
Diese Anordnung hat wohl den Vorteil größter Lichtstärke, weil Absorption und Reflexion auf ein Minimum beschränkt sind, sie soll aber nach Ansicht des Erfinders auch mehrere Nachteile haben.This arrangement probably has the advantage of maximum light intensity because of absorption and reflection are limited to a minimum, but in the view of the inventor they should also have several disadvantages.
Das Blauuviolglas absorbiert nämlich die Wärmestrahlen sehr stark. Infolgedessen erhitzt sich das Filter, seine Kittschichten erweichen und lösen sich. Bei länger andauerndem Gebrauch des Filters muß daher eine besondere Kühlvorrichtung vorgesehen werden. Ferner greifen die Filterflüssigkeiten, besonders die Kupfersulfatlösung, das wenig widerstandsfähige Blauuviolglas an; jedenfalls werden seine polierten Flächen eher zerstört als die des Quarzes oder U.V.-Glases.The blue violet glass absorbs the heat rays very strongly. As a result, heated the filter and its layers of cement soften and loosen. If it lasts for a long time A special cooling device must therefore be provided for use of the filter. Furthermore, the filter fluids, especially the copper sulphate solution, have little effect resistant blue violet glass on; in any case, its polished surfaces are more likely to be destroyed than that of quartz or U.V. glass.
Gemäß der vorliegenden Erfindung werden nun die flüssigen Komponenten getrennt von der Komponente aus Blauuviolglas in einer besonderen Küvette angeordnet, deren Wandungen aus einem für Licht jeder Farbe durchlässigen Material (z. B. Quarz oder U.V.-Kron) bestehen.According to the present invention, the liquid components are now separated from the component made of blue violet glass is arranged in a special cuvette, the walls of which made of a material permeable to light of any color (e.g. quartz or U.V.-crown) exist.
Fig. ι zeigt schematisch eine solche Anordnung im Zusammenhange mit einem Fluoreszensmikroskop. I ist die Lichtquelle, b eine Beleuchtungslinse aus Quarz oder U.V.-Glas, α die gesondert aufgestellte Platte aus Blauuviolglas, d die Küvette aus Quarz oder U.V.-Glas mit den beiden flüssigen Filterkomponenten und c der Beleuchtungsapparat des Fluoreszensmikroskopes. Diese Anordnung hat auch noch den Vorteil, daß man nach Entfernung der Küvette d mit dem Blaüuviolglase als Filter allein beobachten kann, was bei lichtschwachen Objekten (Bakterien, ultramikroskopischen Teilchen) sehr oft zweckmäßig sein kann.Fig. Ι shows schematically such an arrangement in connection with a fluorescence microscope. I is the light source, b an illuminating lens made of quartz or UV glass, α the separately placed plate made of blue violet glass, d the cuvette made of quartz or UV glass with the two liquid filter components and c the lighting apparatus of the fluorescence microscope. This arrangement also has the advantage that, after removing the cuvette d with the blue violet glass as a filter, one can observe alone, which can very often be useful in the case of faint objects (bacteria, ultramicroscopic particles).
Eine vorteilhafte Vereinfachung dieser FiI-ter- und Beleuchtungsvorrichtung wird durch die Fig. 2 dargestellt. Bei dieser Einrichtung ist nämlich die Beleuchtungslinse b1 aus Blauuviolglas gefertigt; sie vereinigt sowohl die Funktionen eines Filters wie die einer Linse in sich. Die dadurch erzielte Verbilligung der Anordnung ist beträchtlich, da die meistenteils für die Zwecke der Beleuchtung verwendeten Ouarzlinsen sehr teuer sind.An advantageous simplification of this filter and lighting device is shown in FIG. In this device, namely, the illumination lens b 1 is made of blue violet glass; it combines both the functions of a filter and a lens. The cheaper arrangement achieved in this way is considerable, since the quartz lenses, which are mostly used for lighting purposes, are very expensive.
• ν• ν
Claims (2)
Publications (1)
Publication Number | Publication Date |
---|---|
DE262432C true DE262432C (en) |
Family
ID=519894
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
DENDAT262432D Active DE262432C (en) |
Country Status (1)
Country | Link |
---|---|
DE (1) | DE262432C (en) |
-
0
- DE DENDAT262432D patent/DE262432C/de active Active
Similar Documents
Publication | Publication Date | Title |
---|---|---|
DE69634963T2 (en) | fluorometer | |
DE102005044580A1 (en) | Arrangement for illuminating a field | |
EP0846663A3 (en) | Device for liquid treatment by UV-irradiation | |
DE3803763A1 (en) | Optical irradition device for biological matter | |
DE262432C (en) | ||
DE3212379A1 (en) | DEVICE FOR TREATING DENTAL MATERIALS | |
DE3200391A1 (en) | OPTICAL DEVICE FOR A FLUORESCENT POLARIZATION DEVICE | |
DE7330229U (en) | DEVICE FOR ADJUSTING THE POSITION AND SIZE OF THE RADIATION FIELD OF AN ROENTGEN DEVICE | |
DE2320937C3 (en) | Optical device for recording the absorption of light in an object which also has a variable index of refraction | |
DE2605156A1 (en) | SCAN ARRANGEMENT | |
DE19823257A1 (en) | Method of definite permanent changing of the extinction spectrum of dielectric-containing metal particles using intensive laser pulses | |
DE682869C (en) | Device for measuring the degree of turbidity of liquids | |
DE1472059A1 (en) | Condenser device for radiation | |
DE1256531B (en) | Auxiliary exposure device for reproduction and enlargement devices | |
DE8209544U1 (en) | DEVICE FOR TREATING DENTAL MATERIALS | |
DE19841888A1 (en) | Filament | |
DE2001087C3 (en) | Device for eliminating the phantom light effect in light signals | |
DE581687C (en) | Method for microscopic examination of living objects | |
DE2104393C3 (en) | Fluorescence analysis device | |
DE529562C (en) | Device for achieving different colored illumination of microscopic preparations | |
AT126714B (en) | Device for achieving different colored illumination of microscopic objects. | |
DE4013133C2 (en) | Lamp monitoring device with solar cell (s), in particular for a UV light for irradiating test objects during magnetic powder or paint penetration testing | |
DE10256808B4 (en) | Projection exposure system | |
AT230122B (en) | Universal condenser for microscopes | |
DE10262076B4 (en) | Device for taking pictures of metallic, partially liquid-wetted surfaces |