DE256116C - - Google Patents
Info
- Publication number
- DE256116C DE256116C DENDAT256116D DE256116DA DE256116C DE 256116 C DE256116 C DE 256116C DE NDAT256116 D DENDAT256116 D DE NDAT256116D DE 256116D A DE256116D A DE 256116DA DE 256116 C DE256116 C DE 256116C
- Authority
- DE
- Germany
- Prior art keywords
- putrefaction
- histidine
- imidazolylethylamine
- pure
- culture
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Active
Links
- HNDVDQJCIGZPNO-YFKPBYRVSA-N L-histidine Chemical compound OC(=O)[C@@H](N)CC1=CN=CN1 HNDVDQJCIGZPNO-YFKPBYRVSA-N 0.000 claims description 13
- 239000000203 mixture Substances 0.000 claims description 5
- 241000894006 Bacteria Species 0.000 claims description 4
- 150000001412 amines Chemical class 0.000 claims description 3
- 238000000034 method Methods 0.000 claims description 3
- 244000005700 microbiome Species 0.000 claims description 3
- HEDRZPFGACZZDS-UHFFFAOYSA-N chloroform Chemical compound ClC(Cl)Cl HEDRZPFGACZZDS-UHFFFAOYSA-N 0.000 claims 2
- BASFCYQUMIYNBI-UHFFFAOYSA-N platinum Chemical compound [Pt] BASFCYQUMIYNBI-UHFFFAOYSA-N 0.000 claims 2
- DEURIUYJZZLADZ-UHFFFAOYSA-N 2-(1H-imidazol-2-yl)ethanamine Chemical compound NCCC1=NC=CN1 DEURIUYJZZLADZ-UHFFFAOYSA-N 0.000 claims 1
- 241000700199 Cavia porcellus Species 0.000 claims 1
- 238000001514 detection method Methods 0.000 claims 1
- 239000012153 distilled water Substances 0.000 claims 1
- 238000000605 extraction Methods 0.000 claims 1
- 150000007523 nucleic acids Chemical class 0.000 claims 1
- 108020004707 nucleic acids Proteins 0.000 claims 1
- 235000015097 nutrients Nutrition 0.000 claims 1
- 230000000144 pharmacologic effect Effects 0.000 claims 1
- 230000035479 physiological effects, processes and functions Effects 0.000 claims 1
- 229940075930 picrate Drugs 0.000 claims 1
- OXNIZHLAWKMVMX-UHFFFAOYSA-M picrate anion Chemical compound [O-]C1=C([N+]([O-])=O)C=C([N+]([O-])=O)C=C1[N+]([O-])=O OXNIZHLAWKMVMX-UHFFFAOYSA-M 0.000 claims 1
- 229910052697 platinum Inorganic materials 0.000 claims 1
- 238000001556 precipitation Methods 0.000 claims 1
- 238000002360 preparation method Methods 0.000 claims 1
- 150000003839 salts Chemical class 0.000 claims 1
- 210000000813 small intestine Anatomy 0.000 claims 1
- 239000011780 sodium chloride Substances 0.000 claims 1
- 238000002255 vaccination Methods 0.000 claims 1
- 229920001817 Agar Polymers 0.000 description 5
- 235000010419 agar Nutrition 0.000 description 5
- 239000008272 agar Substances 0.000 description 4
- 210000000496 Pancreas Anatomy 0.000 description 3
- 239000001888 Peptone Substances 0.000 description 3
- 108010080698 Peptones Proteins 0.000 description 3
- 235000019319 peptone Nutrition 0.000 description 3
- BVKZGUZCCUSVTD-UHFFFAOYSA-N Carbonic acid Chemical compound OC(O)=O BVKZGUZCCUSVTD-UHFFFAOYSA-N 0.000 description 2
- 239000002253 acid Substances 0.000 description 2
- VTYYLEPIZMXCLO-UHFFFAOYSA-L calcium carbonate Chemical compound [Ca+2].[O-]C([O-])=O VTYYLEPIZMXCLO-UHFFFAOYSA-L 0.000 description 2
- PEDCQBHIVMGVHV-UHFFFAOYSA-N glycerine Chemical compound OCC(O)CO PEDCQBHIVMGVHV-UHFFFAOYSA-N 0.000 description 2
- 235000011187 glycerol Nutrition 0.000 description 2
- CSNNHWWHGAXBCP-UHFFFAOYSA-L mgso4 Chemical compound [Mg+2].[O-][S+2]([O-])([O-])[O-] CSNNHWWHGAXBCP-UHFFFAOYSA-L 0.000 description 2
- 239000000047 product Substances 0.000 description 2
- 241000193830 Bacillus <bacterium> Species 0.000 description 1
- VHRGRCVQAFMJIZ-UHFFFAOYSA-N Cadaverine Chemical compound NCCCCCN VHRGRCVQAFMJIZ-UHFFFAOYSA-N 0.000 description 1
- 229960003563 Calcium Carbonate Drugs 0.000 description 1
- 241000206672 Gelidium Species 0.000 description 1
- 229960002989 Glutamic Acid Drugs 0.000 description 1
- WHUUTDBJXJRKMK-VKHMYHEASA-N L-glutamic acid Chemical compound OC(=O)[C@@H](N)CCC(O)=O WHUUTDBJXJRKMK-VKHMYHEASA-N 0.000 description 1
- KDXKERNSBIXSRK-YFKPBYRVSA-N L-lysine Chemical compound NCCCC[C@H](N)C(O)=O KDXKERNSBIXSRK-YFKPBYRVSA-N 0.000 description 1
- 239000004472 Lysine Substances 0.000 description 1
- 210000001541 Thymus Gland Anatomy 0.000 description 1
- 150000007513 acids Chemical class 0.000 description 1
- 235000001014 amino acid Nutrition 0.000 description 1
- 150000001413 amino acids Chemical class 0.000 description 1
- 230000001580 bacterial Effects 0.000 description 1
- 235000014121 butter Nutrition 0.000 description 1
- 229910000019 calcium carbonate Inorganic materials 0.000 description 1
- 230000015556 catabolic process Effects 0.000 description 1
- 238000006243 chemical reaction Methods 0.000 description 1
- 239000003795 chemical substances by application Substances 0.000 description 1
- 230000000875 corresponding Effects 0.000 description 1
- 230000004059 degradation Effects 0.000 description 1
- 239000007857 degradation product Substances 0.000 description 1
- 238000006731 degradation reaction Methods 0.000 description 1
- 239000008121 dextrose Substances 0.000 description 1
- 230000000762 glandular Effects 0.000 description 1
- 235000013922 glutamic acid Nutrition 0.000 description 1
- 239000004220 glutamic acid Substances 0.000 description 1
- 239000001963 growth media Substances 0.000 description 1
- 238000011081 inoculation Methods 0.000 description 1
- 238000009114 investigational therapy Methods 0.000 description 1
- GUBGYTABKSRVRQ-XLOQQCSPSA-N lactose Chemical compound O[C@@H]1[C@@H](O)[C@@H](O)[C@@H](CO)O[C@H]1O[C@@H]1[C@@H](CO)O[C@H](O)[C@H](O)[C@H]1O GUBGYTABKSRVRQ-XLOQQCSPSA-N 0.000 description 1
- 229910052943 magnesium sulfate Inorganic materials 0.000 description 1
- 235000019341 magnesium sulphate Nutrition 0.000 description 1
- PMZURENOXWZQFD-UHFFFAOYSA-L na2so4 Chemical compound [Na+].[Na+].[O-]S([O-])(=O)=O PMZURENOXWZQFD-UHFFFAOYSA-L 0.000 description 1
- 230000001717 pathogenic Effects 0.000 description 1
- 244000052769 pathogens Species 0.000 description 1
- 229910052938 sodium sulfate Inorganic materials 0.000 description 1
- 235000011152 sodium sulphate Nutrition 0.000 description 1
- 238000010186 staining Methods 0.000 description 1
- 239000000126 substance Substances 0.000 description 1
- 210000001519 tissues Anatomy 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07D—HETEROCYCLIC COMPOUNDS
- C07D233/00—Heterocyclic compounds containing 1,3-diazole or hydrogenated 1,3-diazole rings, not condensed with other rings
- C07D233/54—Heterocyclic compounds containing 1,3-diazole or hydrogenated 1,3-diazole rings, not condensed with other rings having two double bonds between ring members or between ring members and non-ring members
- C07D233/64—Heterocyclic compounds containing 1,3-diazole or hydrogenated 1,3-diazole rings, not condensed with other rings having two double bonds between ring members or between ring members and non-ring members with substituted hydrocarbon radicals attached to ring carbon atoms, e.g. histidine
Landscapes
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
Description
KAISERLICHESIMPERIAL
PATENTAMT.PATENT OFFICE.
PATENTSCHRIFTPATENT LETTERING
- JVI256116 -KLASSE Mp. GRUPPE - GROUP CLASS Mp - JVI256116.
Patentiert im Deutschen Reiche vom 4. April 1912 ab.Patented in the German Empire on April 4, 1912.
Ackermann (Zeitschrift für physiologische Chemie Bd. 65 [1910], S. 504, und Bd. 69 [1910], S. 273) hat die Fäulnisprodukte von Aminosäuren einer eingehenden Untersuchung unterzogen. Dabei ergab sich, daß verschiedene dieser Säuren, z. B. Glutaminsäure, Histidin, Lysin, bei der Fäulnis im wesentlichen Kohlensäure abspalten, denn es bilden sich dabei die entsprechenden Amine: y-Aminobutter-Ackermann (Zeitschrift für Physiologische Chemie Vol. 65 [1910], p. 504, and Vol. 69 [1910], P. 273) has subjected the putrefaction products of amino acids to an in-depth investigation. It was found that various of these acids, e.g. B. glutamic acid, histidine, Lysine, essentially split off carbonic acid during putrefaction, because it is formed in the process the corresponding amines: y-amino butter
to säure, ß-Imidazoläthylamin bzw. Pentamethylendiamin. Die Fäulnis leitete Ackermann mit faulender Pankreas bei Gegenwart von Traubenzucker, Pepton, Magnesiumsulfat, Natriumsulfat und einem Überschuß von CaI-ciumcarbonat ein. Diese Methode weist den Nachteil auf, daß neben dem gewünschten Fäulnisprodukt sich noch eine Reihe anderer Stoffe vorfinden, welche die Isolierung der betreffenden Base aus dem Fäulnisgemisch erschweren. Auch scheint die wahllose Anwen-" dung von Fäulniserregern die Ausbeute zu beeinträchtigen, indem wahrscheinlich andere in der faulenden Pankreas sich vorfindende Mikroorganismen einen Abbau in abweichender Richtung bewirken, oder die entstandenen Amine weiter zersetzen. Eine Stütze für diese Annahme liegt in dem Umstand, daß Ackermann bei der Fäulnis von Histidin nicht über eine Ausbeute von etwa 50 Prozent an ß-Imidazolyläthylamin hinauskommt. Dieser Übelstand läßt sich, wie im Patent 252872 gezeigt ist, dadurch zum größten Teil beheben, daß besondere Fäulnisgemische verwendet werden und der Fäulnisprozeß auf 4 bis 6 Tage abgekürzt wird.to acid, ß-imidazolethylamine or pentamethylenediamine. The putrefaction caused Ackermann with putrefying pancreas in the presence of Dextrose, peptone, magnesium sulfate, sodium sulfate and an excess of calcium carbonate a. This method has the disadvantage that in addition to the desired putrefaction product, there are a number of others Find substances that make it difficult to isolate the base in question from the putrefaction mixture. The indiscriminate use of putrefactive agents also seems to increase the yield affect by likely others found in the rotting pancreas Microorganisms cause degradation in a different direction, or the resulting Further decompose amines. Support for this assumption lies in the fact that Ackermann was involved in the putrefaction of histidine does not exceed a yield of about 50 percent of ß-imidazolylethylamine. This As shown in patent 252872, the problem can be largely remedied by that special putrefaction mixtures are used and the putrefaction process is shortened to 4 to 6 days.
Es wurde nun gefunden, daß die Abspaltung von Kohlensäure aus dem Histidin noch sicherer durchgeführt werden kann, wenn man eine Reinkultur der kohlensäureabspaltenden Mikroorganismen zur Fäulnis verwendet. Auf diese Weise gelingt es, die Reaktion auf der gewünschten Stufe anzuhalten.It has now been found that the splitting off of carbonic acid from the histidine is still possible Safer can be done if you have a pure culture of the aerosolizing Microorganisms used to putrefaction. In this way it is possible to get the reaction on the desired level.
Durch systematische Züchtung von Reinkulturen auf verschiedenen Nährböden (Agar, Endoagar, Glycerinagar) gelingt es, aus dem nach Ackermann oder aus dem im Patent 252872 erwähnten Fäulnisgemisch einen coccenähnlichen Bacillus zu isolieren, der imstande ist, Histidin unter Ausschluß jedes anderen Fäulniserregers derart abzubauen, daß sich das therapeutisch wertvolle ß-lmidazolyläthylamin als wesentliches Abbauprodukt bildet.Systematic cultivation of pure cultures on different culture media (agar, Endoagar, glycerine agar) succeeds from the Ackermann or from the patent 252872 mentioned putrefaction mixture to isolate a cocci-like Bacillus, which is capable is to break down histidine with the exclusion of any other putrefactive pathogen in such a way that the therapeutically valuable ß-imidazolylethylamine forms as an essential degradation product.
Zur Gewinnung des Bakteriums wird kernreiches Drüsengewebe (Pankreas oder Thymus) bei 37° der Fäulnis überlassen. Von der dabei sich üppig entwickelnden gemischten Bakterienflora wird auf die schwach alkalische Lösung der mit etwas steriler Bouillon oder Pepton versetzten Histidinlösung übergeimpft. Hierbei entwickeln sich vorzugsweise kurze Stäbchen neben coccenartigen Gebilden. Beim weiteren Überimpfen auf Agar-Agar, Glycerinagar, Milchzuckeragar sowie auf peptonhaltige Histidinlösungen entwickelt sich ausschließlich ein coccenartiges Stäbchen, das bisweilen in der Art von Diplococcen oder Streptococcen Kolonien bildet. (Gramsche Färbung negativ). Das Bakterium wächst spärlich auf Histidinlösungen allein, üppig in schwach alkalischen Lösungen des Histidins,Nuclear-rich glandular tissue (pancreas or thymus) is used to obtain the bacterium left to rot at 37 °. From the abundantly developing mixed bacterial flora is inoculated onto the weakly alkaline solution of the histidine solution mixed with some sterile broth or peptone. Here, preferably short rods develop next to cocci-like structures. At the further inoculation on agar agar, glycerin agar, milk sugar agar and peptone-containing Histidine solutions develop exclusively a cocci-like rod, which is sometimes in the manner of Diplococci or Streptococci forms colonies. (Gram's staining negative). The bacterium grows sparingly on histidine solutions alone, abundantly in weakly alkaline solutions of histidine,
Claims (1)
Publications (1)
Publication Number | Publication Date |
---|---|
DE256116C true DE256116C (en) |
Family
ID=514162
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
DENDAT256116D Active DE256116C (en) |
Country Status (1)
Country | Link |
---|---|
DE (1) | DE256116C (en) |
-
0
- DE DENDAT256116D patent/DE256116C/de active Active
Similar Documents
Publication | Publication Date | Title |
---|---|---|
EP0012278A2 (en) | Preparation of N-substituted derivatives of 1-deoxy-nojirimycine | |
DE3541581A1 (en) | METHOD FOR PRODUCING MUCONIC ACID | |
DE256116C (en) | ||
DE3689174T2 (en) | Process for the preparation of riboflavin. | |
DE1231653B (en) | Process for the production of 1-homoserine by fermentation | |
DE2059277C3 (en) | Microbiological process for the production of high quality protein | |
DE1174286B (en) | Process for the biotechnological production of 1-isoleucine | |
DE228592C (en) | ||
DE1009583B (en) | Process for the biochemical production of 2-keto-1-gulonic acid | |
DE2849393C2 (en) | Process for the biotechnological production of 2,5-diketogluconic acid | |
DE252872C (en) | ||
DE2366505C2 (en) | Use of the strain Pseudomonas ATCC 21973 for the in situ production of an aminotransferase | |
DE274681C (en) | ||
DE814890C (en) | Process for the production of butanediol (2, 3) and butanolone (2, 3) by fermentation | |
DE1107225B (en) | Process for the production of 1, 4, 17 (20) -Pregnatrienes | |
DE2156339A1 (en) | Process for the production of L-asparaginase | |
AT224265B (en) | Process for the production of Laevansucrase | |
DE289687C (en) | ||
DE1939035C3 (en) | Process for the production of xylitol by microbiological means | |
DE664428C (en) | Process for the production of tanning extracts from sulphite cellulose waste liquor | |
DE3712539A1 (en) | Process for the microbial production of L-alpha-amino acids | |
DE1041896B (en) | Process for the production of amino acids by biotechnological means | |
DE1122949B (en) | Process for the preparation of 16ª ‡ -Methyl-11ª ‡, 17ª ‡ -dioxy-4-pregnen-3,20-dione | |
DE1793806B1 (en) | PROCESS FOR PRODUCING L-ALANINE | |
DE1643539A1 (en) | Process for the preparation of D-pantoic acid |