DE2535296A1 - Protein prodn. by metabolisation of liquid manure - also produces agriculturally-useful disease-free products cheaply - Google Patents

Abstract

Liq. manure (I) is metabolised to proteins using microorganisms by (a) subjecting (I) to enzymatic attack with pancreatic, bacterial or fungal protease or tripsinogens at neutral pH, (b) inoculating the (I) resulting from (a) with a massive culture of >=1 first Hyphomycetes selected from Ascomycetes and micro-algae and fermenting at pH 3-7.5, (c) sepg. a protein-rich sludge from (b), (d) re-inoculating (I) remaining after (c) with a second strain of Ascomycetes and micro-algae and aerobically fermenting at pH 3-7.5 and (e) sepg. a second protein-rich sludge fraction from the (I) in (d) leaving purified water suitable for fertilising irrigation. The process produces agriculturally-useful products which are disease-free cheaply. The protein component, mineral salts, disinfectants and antibiotics produced in the course of metabolism are mullified in (a) and accelerate the process overall. Use of the products of depolymsn. or dextrinisation of the starches recovered during the process to equilibriate the nutrient factors of the microflora reduces costs of adding extra carbohydrate (since these are no longer assimilable by the epiphytic microflora present in (I). Costs are also reduced because the lactic acid produced during the process can be used to regulate the pH instead of other acids.

Description

Process for the production of proteins by metabolizing Manure The present invention relates to a process for the production of noble proteins, by having biological sludge, especially liquid manure, metabolized by Subjects to microorganisms.

Liquid manure, especially that from intensive breeding farms, comes from Cleaning of stables, birthing halls, slaughterhouses and urea catabolism and partly from the faecal catabolism of the animals won in the pig breeding farms, whereas those won the end of P5erde- und cattle breeding Wdr are partly transported into the water drains.

There is also a certain amount of residual feed in the manure of a rearing plant and disinfectants used for cleaning the stables, with the result that the wastewater is heavily enriched with teeming matter.

The traditional treatment method based on the active sludge system for manure consists in that the manure in a basin by means of a centrifuge or a compressor and a part of the generated sludge in the circuit is returned.

If the above procedure is used, the following information is provided obtained from a sample analysis: Liquid manure entering from the plant outflowing water pH 7.2 7.9 mg% dry residue 0.49% total nitrogen (after Kjeldahl) 60 mg% 44 "ammonia nitrogen (according to Nesler) 45" 36 "amine nitrogen (according to Von Slike) 8 "4" protein nitrogen (method Low after dialysis) 9 "1.1" Nitrites (according to Gries) 0.1 "0.1" Nitrates (Brucine method) (absent) (traces) Organic Carbon (bichromate method) 43 mg% 7 mg% total phosphorus 0.5 "0.36" total microbial use 3.8 x 109 germs / ml 1.8 x 108 germs / ml proteolytic substances 9 x 10² "" 5 "" Ammonizing Substances 9.5 x 106 "" 9.5 x 106 "" Nitrosating Substances 600 "" (absent) Nitriding substances 0.6 "" 2 "" BOD5 untreated 730 p. p. m.

BOD5 with inoculation 1250 p. p. m.

Total colombacteria (on lactose-added broth) 1.5 x 10³ "" Faecal Colomb Bacteria 4.5 x 10³ "" The information above shows that the microbial ingestion of the ammoniating proteolytic substances does not changes, which allows the conclusion that the activated sludge system is forced aeration the reproduction of a monobophage microflora requires, whereas a syrnbiotic one There is no epiphytic microflora present. That means welters an unbalanced ratio of C / N, as is also the case with the data in the table it can be seen, according to which the proportion of total nitrogen both on entry as well as when leaving because of the strong activity of the ammoniating substances, which are always present remains practically unchanged, whereas the organic content Carbon shows a considerable decrease.

In summary, it can be said that the active sludge process is ineffective because the sludge formed is not free of pathogens and the wastewater still have a considerable degree of pollution, which is the same makes useless.

The main object of the present invention is to provide an effective one Process which enables biological sludges in general and in particular Manure by means of metabolism by microorganisms in particular for loin farming to convert useful products, such as into one rich in valuable proteins Sludge and wastewater with a low level of pollution, so it's dung-end Irrigation can be used.

Another object of the invention is to provide a method to metabolize Gu11le, which makes it possible to obtain products that free from pathogens are and therefore a greater security for man and for the plant kingdom.

Another object of the invention is to provide an improved II Process for the metabolism of manure in order to obtain high quality proteins, this process allows the macromoleculars generally contained in manure Compounds, namely proteins, disinfectants and antibiotics to a large extent break down in order to produce them which can easily be similated by microbial cells are to convert and to their antagonistic and slowing down the metabolism process To cancel the effect.

Finally, another object of the invention is to provide one Process for the metabolization of manure, which from the economic standpurkt is particularly beneficial from a perspective, as there is a reduced intake of nutritional factors the metabolizing microflora consist of additional substances or substances, which regulate the pH value in the various process phases, etc. @requires.

These and others can be seen more clearly in the following description The method according to the invention for the production of proteins achieves goals achieved by metabolizing liquid manure by means of microorganisms, which thereby is characterized in that a) the manure is subjected to an enzymatic attack by a protease, selected from the group comprising pancreatic proteases, bacterial proteases, fungal proteases and tripsinoglenes, subjected at a substantially neutral pH, b) the Liquid manure of phase a) selected with a solid culture of at least one first strain from the group comprising ascomycetes and microalgae inoculated and the manure from a first fermentation caused by said first strain at a pH of 3 to 7.5, c) the gulle of phase b) a first, separating the protein-enriched sludge fraction, d) the remaining manure from the Phase c) with a massive culture of at least one second strain, au.sgewahl + ans of the group comprising ascomycetes and microalgae, inoculated and in the manure one second fermentation effected by said second strain at pH from 3 to 7.5 evokes under aerobic conditions and e) from the manure of the phase d) a second, protein-enriched sludge fraction and for fertilizing irrigation separating suitable, purified water.

The method according to the invention preferably provides a further phase before that in the production of massive cultures of the first and second strains through Inoculation of parts of the manure from phase a mixed with dextrinized starch, with the tribes concerned.

Other features and advantages of the invention are more apparent as follows Description of a preferred embodiment of the method according to the invention for the production of proteins by metabolizing manure with reference to Attached drawing, in which: Fig. 1a and 15 together a flow chart show, which schematically illustrates an embodiment of the method according to the invention.

In the process according to the invention, any biological starting material can be used Mud used, be it now Manure from farms, in particular Intensive breeding facilities, be it mud from public sewers. As an example is the application of the procedure to liquid manure from intensive pig breeding establishments is explained. The manure collected, for example, in a collecting basin 1 is preferred initially of suspended solids such as straw, sawdust, faecal residues, etc. freed by means of filtration through II a vibrating sieve 2. The liquid part or the actual manure, which was separated by the vibrating sieve 2, is a digester 3 where it is subjected to an enzymatic attack to remove the contained Break down proteins, amides, disinfectants and antibiotics. To this end is contained in the Digestor 3 and usually one lying in the alkaline range Gulle with pH value by adding acid from a special container 4 is fed via a metering pump 4a, to an essentially neutral pH Brought value. Although any organic or inorganic acid can be used can, one preferably uses lactic acid, which, as will be explained later, in the invention Process itself is generated. Then the manure is treated with a protease. All pancreatic proteases, bacterial proteases and fungal proteases are suitable for this purpose as well as tripsin or tripsinogens in general. You get good results with an Eakterier½ £ rotease such as z0B0 Alphachymotrypsinogen Der floodable at the university for animal breeding by Piacenza under the designation HT Proteolytie 200, The working temperature is not critical and can range between room temperature and 800 for treatment times from 1 to 12 hours, depending on the temperature used. The proteolytic Attack is carried out by putting the manure mass in Digestor 3 for example with a mechanical stirrer stirs and slowly breathe in air. Since amino acids and organic acids are formed by the roteolytic degradation, which cause a drop in the pH value, it becomes more organic when added or inorganic bases from a special container 5 by means of a metering pump 6 held at a constant neutral value. In the described embodiment ammonium carbonate solutions are preferably used. The action of the protease is followed by taking guillotine samples from the Kjeldahl protein nitrogen analysis Subjects. @ The disappearance of nitrogen determined by this method shows the end of the phase of enzymatic attack on the manure.

The manure exposed to proteolytic degradation in Digestor 3 becomes fermented with protein-producing hyphomycetes in the following manner. The hyphomycetes, which are the main metabolic strains in the process according to the invention are equal to every microbial cell of an aging and consequently a genetic one Subjected to mutation in the course of its ana-catabolic action. This is a consequence various factors, such as microbial antagonism, causing the in the inoculated a liquid containing a specific epiphytic microflora. Microorganisms experience a slowdown in their splitting process. For this (round st your Inoculation in massive form necessary. Moreover, the antagonism and the metabolizing Slowing down to genetic mutations that affect the nature of the obtained Change the mud. Therefore, the present invention conveniently comprises one Phase of massive production of metabolizing strains or hyphomycetes.

According to the invention, it was found in this context that massive production of hyphomycetes can advantageously take place by using the narcotic factors The hyphomycetes do not use simple carbohydrates, which are easily made by the bacteria the epiphytic microflora are assimilated and therefore used in large quantities need to be, but balances with a nutritional element that is specific and only is easily assimilated by the hyphomycetes. This specific for the hyphomycetes Nutritional element consists of dextrinized starch and the method according to the present one The invention expediently comprises a dextrinization phase of the starch.

Although all starchy plant products such as corn, potatoes, Beans, rice, rye, oats, sona, etc. can be used with advantage rice is used as a source of starch, which is suitable for this type of grain due to its content typical growth factors as best suited to nutritional requirements who has demonstrated biomass inoculated with hyphomycetes. The dextrinization of the Starch is made by treating the rice with an amylase, its concentration preferably in a range between 0.2 and 1 weight- based on that to be treated Dry product is calculated, in the presence of an enzymatic activator, whose Concentration preferably in a range between 1 and 8 Vo] '.%, Calculated on the total volume of the mass subjected to dextrin sieving. As amylase can be any amylase selected from the group of the pancreatic amylases, bacterial amylases and fungal amylases can be used, in the embodiment described with advantage the bacterial amylase alpha 1,4-glucan-4-glucan hydrolase, available at of the University of Animal Breeding of Piacenza under the name SP 250 Amylase. The enzymatic activator consists of a metal salt. To this end are organic and inorganic salts of Zn, Ca, Fe, Al, Mg, Co, Ni, n, Ti etc. in Mixture with an alkali or alkaline earth phosphate, preferably potassium phosphate, suitable.

The dextrinization takes place in the digester 7 after the rice has been cooked at atmospheric or pressure. The dextrinization treatment is carried out at a temperature which can be between room temperature and 800C, depending on the treatment time according to the temperature used, carried out between 15 minutes and 6 hours. The dextrinization can be carried out under optional semi-anaerobic conditions by stirring the material slowly or statically in the dextrinizing vessel holds. The dextrinization can be considered finished when the mass has a has assumed sufficient flowability and the starch granules at least 9 Have disappeared.

Part of the dextrinized starch produced in this way is used for Production of lactic acid, which is also included in the process according to the invention is used. Lactic acid is a factor in regulating the pH value during the various phases of the process, whereby the addition of purchased acids is advantageously restricted. It therefore becomes part of the dexstrinized starch removed from the dextrinization vessel 7 and fed to a sterile digester 8, where he mlt malt extract, calculated to 1.5 - 3% of his weight, added and with a culture of Lactobacillus Delbrueckii available from the University of Piacenza under the designation SB / UP 187 and grown on Pasteurive broth or on any other specific nutrient broth, is inoculated. The fermentation can be done with or without panren.

and the working temperature varies from room temperature to 45-50 ° C. Instead of lactobacillus Delbrueckii can be anyone else Stem of the group specified below (the list is the Identifier of the University of Piacenza added): Lactobacillus Bulgaricus SB / UP 188; "Helveticus" 189; "Caucasicus" 190; "Casei" 191; "Plantarum "192;" Sili "193;" Lactis "194;" Thermophilus "195;" Acidophilus "196; "Bifidus" 197; "Leichmanii" 198; "Brevis" 199; "Buchneri" 200; "Fermenti "201;" Pasteurianus "202;" Mannitopoeus "203; Gracilis lt 204;" Intermedius "205;" Gayonii "206;" Trichodes "207;" Hilgardii "208;" Pavarii "209; Bacterium Coli "210; Escherichia Coli" 211; Bacterium Freundii "212; Escherichia Freundii "213; Bacterium Intermedium" 214; Eschrerichia Intermedia SB / UP 215; Bacterium Aerogenes "216; Bacterium Acidi lactici" 217; Bacterium Cloacae "218; Aerobacter Cloacae" 219; Bacterium Proteiforme "220; Aerobactrer Proteiformis "221; Streptococcus Lactis" 222; "Faecalis" 223; "Glicerinaceus" 225; "Proteiformis "226;" Cremoris "227;" Bovis "228;" Inulinaceus "229;" Agalactiae "230; "Thermophilus" 231; "Durans" 232; "Hemothermophilus" 233; "Liquefaciens" 234; "Masenterioides" 235; "Destranicus" 236; "Paracitrovorus" 237; "Citrovorus "238; Enteroccocus Proteinformis" 239; Leuconostoc Mesent "240;" testranicum "241," Citrovorum "242; Betacoccus Arabionosaceus" 243; "Bovis" 244; "Cremoris "245; The lactic acid becomes discontinuous in the process according to the invention -, n depending on the demand occurring during production and in a special E'ehalter 9 gelagart, from where by means of metering pumps on the is distributed in different production phases.

The remaining de-extruded starch from the dextrinizing vessel 7 is then fed to the massive production phase of metabolizing strains, selected from the group of ascomycetes and microalgae. In a first Digestor 10 will mass produce a first strain or hyphomycete of the following group: I II SACCHAROMYCES CEBEVISIAE SC / ISM 10013; -RHODOTORULA GLUTINIS "10017; -LYPOMICES LIPOFERUS" 10018; SB / UP 185 TRICHOSPORAN PULLULANS "10019; -CHLORELLA VULGARIS" 10022; SB / UP 186 GLIOCLADIUM CATENULATUM --------- "160 BACILLUS COMESII ---------" 161 SPOROCYTOPHAGA MYXOCOCCOIDES --------- " 162 "ELLIPSOSPORA ---------" 163 "CONGREGATA ---------" 164 CYTOPHAGA WINOGRADSKY --------- "165" FLAVICULA --------- "166" CROCEA --------- "167" ALBOGILVA --------- "168 BACILLUS MACERANS-ii ASTE @@ SPORITS ~~~~~ 170 PECTINOBACTER AMYLOPHILUM --------- "171 STREPTOMYCES RUBRIRETICULI ---------" 172 AZOTOBACTER AGILE --------- SB / UP 173 "VOOUSTOWNII ---------" 174 "HILGARDII ---------" 175 CLOSTRIDIUM OMELIANSKII --------- "176" FLAVUM --------- "177 METHANOMONAS VANNIELII --------- "178 SARCINA METHANICA ---------" 179 CLOSTRIDIUM BIFERMENTANS --------- "180" SPOROGENS --------- "181" VIRENS --------- "182 CANDIDA AU3ICÄNS iv 183 "iFLAYBI 11 184 The designations in the first column (I) and in the two Column (II) are the identification designations of the tribes concerned Istituto Sieroterapico Milanese and at the University of Animal Breeding of Piacenza. in iv the embodiment described, Saccharomyces was advantageously Cerevisiae, strain SC / ISM 10013, was used. The massive split of this tribe was made by using a portion of dextrinized starch as the digester 7 and one Part of the manure exposed to the enzymatic attack in the Digestor 3 as a culture broth achieved.

For this purpose, the liquid manure removed by the Digestor 3 was first used one that is optimal for the development of the Saccharomyces Cerevisiae SC / ISM 10013 strain Brought pH, which is in a range of 3 - 7.5. The setting of the pH value is in a collecting container 11 with lactic acid removed from the storage container 9 performed. The mixture of a fraction of dextrinized coming from the digester 7 Strong and part of the manure coming from the collecting tank 11 is thus regulated, that the ratio o / n is balanced to values, which lie in the optimal growth interval of the strain in question. During the By splitting the strain, the pH value becomes constant at values between 3 and 7.5 All lactic acid added and the culture is constantly aerated. The split process takes 48 hours. After this time, a predominant part, like about 3/4 of the The material contained in the Digestor 10 is passed on for the metabolism of the manure, whereas the remaining 1/4 is used for the next inoculation. Another Share of dextrinized starch from the digester 7 is a digestor 12 for the Mass production of an amylolytic microorganism used this amylolytic Microorganism is preferably used to promote metabolism through symbiotic action a second metabolizing one. Tribal or hyphomycetes, as will be explained later is going to begin.

In the present case it was preferred as an amylolytic microorganism the strain Endomycopsis Fibuliger, available from the Istituto Sieroterapico Milanese used under the designation SC / ISM 10011. Digestor 12 is then dextrinized with Strong and filled with manure coming from the collecting tank 11. These components are mixed in such proportions that one suitable for the growth of the trunk Ratio C / N is achieved. To balance the nutritional factors, you can also depending on the special requirements depending on the composition of the Slurry, trace elements such as Mg, E phosphates, etc. are added 0 After balancing of the nutritional factors, the pH is adjusted to a value of 3 to 7.5 by adding acids (Lactic acid) or bases (any base) and throughout the fermentation on the its values. Now is in the Digestor 12 with a Inoculated iv dense culture of Endomycopsis fibuliger grown on Difco broth and the digester ventilates slowly. The growth cycle is over in 48 hours and the digester is emptied by 3/4 of its volume, what proportion a digester 13 is supplied.

In the Digestor 13 the massive production of the second strain or takes place second Hyfomycetes selected from the following group: strain identification Identification designation, Istituto designation, Uni-Sieroterapico Milanese versity the breeding of Piacenza CANDIDA UTILIS SC / ISM 10012; "ALBICANS - - SS / UP 183 "TROPICALIS - 10015; -" FLAVERI - - SB / UP 184 In the present case, Candida Utilis strain SC / ISM 10012 used; this strain is based on the products of catabolism Fermented by Endomycopsis Fibuliger, in the Digestor 13 becomes sweeter that of the Digestor 12 coming fermentate a small portion sn nitrogenous salts or manure added from the collecting tank 11 to bring the ratio C / N to that for this strain to bring ideal growing conditions. The pH is based on that for growth of the trunk optimal values of 3 - 7.5 are set and during the entire duration of the process automatically by an attached acid or vi Base addition kept at this value. The growth cycle is completed in 48 hours, after which the digester closes 3/4 of its volume is emptied, which Part of the metabolism is fed to the manure, whereas the remaining 1/4 of the Volumnes for the following inoculation in the Digestor 13 is used. The metabolizing thus produced Strains are fed into the metabolism phase of the manure in order to convert the latter into proteins and get purified water. The C-ulle coming from the collecting container 11 is fed to a first fermentation tank 14, where it is 3/4 of the Contents of the digester 10 for the massive production of said first strain Saccharomyces Cerevisiae is added. The pH is adjusted by adding lactic acid to the optimum for the Saccharomyces Cerevisiae strain, in the range from 3 to 7.5 values are set and the metabolism is expediently below Conditions for facultative aerobics by slowly blowing in tuft by means of the compressed air distribution line 15 carried out. $ Of course you can in view a balanced C / N ratio before the start of the fermentation process, depending on the requirements carbon, nitrogen or phosphorus introducing substances admit. The process takes 12-48 hours, depending on the type of food being metabolized Substrate. After this period of time, the manure is fed to a centrifuge 16, where a first sludge fraction ii enriched with ar proteins is separated from it. The part floating or liquid on the surface which is carried out by the centrifuge 16 Was separated, a further fermentation tank 17 is fed, where he is one second fermentation by the mentioned second strain is suppressed. in the fermentation vat 17 is the liquid thrown off by the centrifuge 16 Metabolized by the Candida utilis strain. This metabolism is carried out under inevitable aerobics. Fermentation takes place, which is the Addition of more Substances for adjusting the pH value and the proper balance of nutritional factors, in the same way like the first fermentation in basin 14. After blowing in for 12-48 hours Air, the time depends on the particular substrate to be metabolized, is the Perwentation completed and the content of the fermentation basin 17 becomes one Centrifuge 18 is fed where a second fraction of protein enriched Sludge and a liquid part or part floating on the surface, that of a flocculation device 19 is supplied, separated.

Those separated from centrifuges 16 and 18 and enriched in proteins Sludge fractions are fed to a sterilizer 20, where they add up to 5 volumes of water to be added.

which has been alkalized with Ca (OH) to a pH of 10-11. Of the The contents of the sterilizer are brought to a boil and at boiling temperature for 15-30 minutes held to induce cellolysis and all epiphytic microorganisms present to kill. When the sterilization is complete, the solids are centrifuged abge separated, dried in a lukewarm air stream and then ground and the production for high-protein feed. The obtained, having a high nutritional value Protein sludge was tested for protein content at the University of Animal Breeding analyzed by Piacenza.

The analysis values are given below: dry matter 96, 25%; Crude proteins 40.96% ss; Ether extract 5.49% ss; Ashes 7.8% as; pH 6; N N 0.042% see total amino acid content (1st value:% total amount, 2nd value% pro teide): Lysine: 2.97, 7.57; Ilystidine: 0.74, 1.87; Arginine: 1.70, 4.30; Triptophan: 0.47, 1.19; Aspartic acid: 3.86, 9.77; Treonine: 1.99, 5.04; Serine: 1.99, 5.04; Glutamic acid: 5.85, 14.82; Proline: 1.80, 4.56; Glycine: 2.05, 5.19; Alanine: 2.45, 6.20; Cystine: 0.52 1.31; Valine: 2.27, 5.75; Methionine: 0.92, 2.33; Isoleucine: 1.89, 4.79; Leucine: 2.53, 6.40; Tyrosine: 0.16, 0.40; Phenylalanine: 0.19, 0.59; Hydroxyproline: 1.00, 2.44; Digestibility according to the pepsin-hydrochloric acid method: 82%.

The liquid residue remaining after the sludge has been separated from the manure In the flaker 19 a separation of inorganic salts, which are not caused by the microbial Cells were metabolized, subjected. To this end, the liquid fraction is used alkalized, for example, by adding Ca (OH) 2 in aqueous suspension, after which polyelectrolytic flocculants such as acrylates, ferrous sulfate, ferric chloride, etc. can be added. After the iv flocculation, the liquid fractions with a Rotary filter or a filter press or any other physical filter system filtered, resulting in a separation of a sludge that is used, for example, in fertilizer production is supplied, and the separation of clear, essentially purified water, after adjusting the pH to a neutral value for fertilizing irrigation is used, is achieved.

The above embodiments show that with the inventive Procedure, the objectives set at the beginning can be achieved completely handily. It enables the extraction of products useful for agriculture, such as proteins rich sludge free from pathogens and clear, purified Water with largely reduced levels of pollution, which is the fertilizing Irrigation is suitable. If you have the manure before fermentation by Subjecting hyphomycetes to enzymatic attack by a protease will become the Antagonism iod slowing down the metabolism process by protein components, Mineral salts, disinfectants and antibiotiw ka, which are used by the metabolizing Trunks are not dismantled, canceled and thus an acceleration of the Overall procedure achieved. Furthermore, due to the proteolytic degradation, they become organic Acids and amino acids free and consequently the need for adenosine triphosphoric acid and phosphates decreased to balance the nutritional factors of the metabolizing strains. The use of products of depolymerization or dextrinization the strength, which are obtained in the course of the process according to the invention, such as specific carbohydrates to balance the nutritional factors of the microflora the cost of performing the carbohydrates as these are no longer produced naturally in the gulle are consumed in the presence of epiphytic microflora. Furthermore, the economy of the process by using the lactic acid produced in the process itself increases that in place of other acids to regulate the pH in the various Stages of the procedure can be used.

The invention can be modified and varied in many respects, without going beyond its scope.

Claims (9)

P A T E N T A N S P R Ü C H E 1. Process for the production of proteins by means of the labeling of G2lle through micro-organisms, d u r c h e k e n n nz e i c h n e t that one a) the manure undergoes an enzymatic attack by a protease selected from the group including pancreatic proteases, bacterial proteases, fungal proteases and tripsinogens, at a substantially neutral pH, b) the slurry of the phase a) with a massive culture of at least one first hyphomycete selected from the group comprising ascomycetes and microalgae inoculated and the manure of a first, filtration brought about by the aforementioned first strain at a pH of 3 up to 7.5 subjects, c) from the liquid manure of phase b) a first, enriched in proteins Separates sludge fraction, d, the remaining liquid manure of phase c) with a cassive culture at least one second strain selected from the group comprising ascomycetes and microalgae, encased and in the manure a second, through the mentioned second Strain caused termentation at pH 3 to 7.5 under aerobic conditions and e) from the manure of phase d) a second, enriched in proteins Separates sludge fraction and purified water suitable for fertilizing irrigation. 2, method according to claim 1, d u r c h g e n n n z e i c h n e t that it has an additional phase, which is that the @essive cultures of the first and second trunks are made by removing parts of the: manure of the Phase a) inoculated with the strains concerned in a mixture with dentrinized starch will. 3. The method of claim 1, d a d u r c h g e k e n n z e i c h n a t that the protease in phase a) is a bacterial protease, alphachymotrypsinogen, available at the University of (Animal Breeding of Piacenza under the designation HT-Proteolytie 200, is. 4. The method according to any one of claims 1-3, d a d u r c h g e k e n Nz e i c h e t that the mentioned first strain in phase b) from the group comprising; SACCHAROMYCE CEREVISIAE; RHODOTORULA GLUTINIS; LYPOMICES LIPOFERUS; TRICHOSPORAN PULLULANS; CHLORELLA VULGARIS; GLIOCLADIUM CATENULATUM; BACILLUS COMESII; SPOROCYTOPHAGA MYXOCOCCOIDES; SPOROCYTOPHAGA ELLIPSOSPORA; SPOROCYTOPHAGA CONGREGATA; CYTOPHAGA WINOGRADSKY; CYTOPHAGA FLAVIOULA; CYTOPHAGA CROCEA; CYTOPHAGA ALBOGILVA; BACILLUS MACERANS; BACILLUS ASTEROSPORUS; PECTINOBACTER AMYLOPHILUM; STREPTOMYCES PUBRIRETIOULI; AZOTOBACTER AGILE; AXOTOBACTER VOODSTOWNII; AZOTOBACTER HILGARDII; CLOSTRIDIUM OMELL @ NSKII; CLOSTRIDIUM FLAVUM; METHANOMONAS VANNIELII; SARCINA METHANICA; CLOSTRIDIUM BIFERMENTANS; CLOSTRIDIUM SPOROGENS; CLOSTRIDIUM VIRENS; CANDIDA ALBICANS; CANDIDA FLAVERI, from ~ is chosen. 5. The method according to any one of claims 1 to 4, d a d u r c h g e k It is noted that the mentioned second phase d) from the group comprises Identification designation at the Istitute Siero designation the terapico Milanese University of the Zoo Tribe --------------------- technique of Fiacenza CANDIDA UTILIS SC / ISM 10012; -CANDIDA AIBICANS ------ SB / UP 183 CANDIDA TROPICALIS SC / ISM 10015; -CANDIDA FLAVERI ---------- SB / UP 184 6th Method according to claim 2, d u r c h e k e n n n z e i c h n e t that the production of the second strain takes place by dividing parts of the manure of phase a) with the second Strain mixed with catabolic products obtained by fermentation of Dividing the gulle of phase a) and dextrinized starch by means of an amylolytic Microorganism consisting of ENDOMYCOPSIS FIBULIGER, available from the Istituto Sieroterapico Milanese under the name SC / ISM 10011. 7. The method according to any one of claims 1 to 6, characterized in that that it has an additional phase for the production of dextrinized starch. which consists in the fact that a starch source is susceptible to enzymatic attack by an amylase, selected from the group comprising pancressamylases, bacterial amylases, and fungal amylases in the presence of an enzymatic activator, consisting of a Mischujig one or more organic and / or inorganic metal salts with one or more Alkali and / or alkaline earth metal phosphates 8. The method according to claim 7, characterized in that the amylase from the bacterial amylase alpha-1,4-gluken-4-glucan hydrolase, available from the University of Animal Breeding of Piacenza under the symbol SP 2O AMYLASE, consists. 9. The method according to any one of claims to 8, characterized in that that there is an additional phase of the production of lactic acid as a means for that Having control of the pH value in the process, which consists in the fact that one dextrinized Strong in a mixture with 125 to 3 % By weight based on weight the starch, mole extract, a fermentation at 20 to 50 ° C, caused by a or more of the following strains: LACTOBACILLUS DELBRUECKII SC / ISM 157; "BULGARIUCUS SB / UP 188;" HELVETICUS "189;" CAUCASICUS "190;" CASEI " 191; "PLANTARUM" 192; "SILI" 193; "LACTIS" 194; "THERMOPHILUS" 195; "ACIDOPHILUS "196;" BIFIDUS "197;" LEICHMANII "198;" BREVIS "199;" BUCHNERI "200;" FERMENTI "201;" PASTEURANUS "202;" MANITOPOEUS "203;" GRACILIS "204;" INTERMEDIUS "205;" GASYONII "206;" TRICHODES "207;" HILGARDII "208;" PAVARII "209; BACTERIUM COLI "210; ESCHERICHIA COLI" 211; BACRTERIUM FREUNDII "212; ESCHERICHIA FREUNDII "213; BACTERIUM INTERMEDIUM" 214; ESCHERICHIA INTERMEDIA "215; BACTERIUM AEROGENES "216; BACTERIUM ACIDO LACTICI SB / UP 217; "CLOACAE" 218; AEROBACTER CLOACAE "219; BACTERIUM PEOTEIFORME" 220; AEROBACTER PROTEIFORMIS "221; STREPTOCOCCUS LACTIS" 222; "PAEOALIS" 223; "FAECIUM" 224; "GLYCERINACEUS" 225; "PROTEIFORMIS" 226; "CREMORIS" 227; "BOVIS" 228; "INULINACEUS" 229; "AGALACTIAE" 230; "THERMOPIDUS" 231; "DURANS" 232; "HEMOTHERMOPHILUS" 233; "LIQUEFACIENS" 234; "MESENTERIOIDES" 235; "DESTRANICUS" 236; "PARACITROVORUS "237;" CITROVORUS "238; ENTEROCOCCUS PROTEIFORMIS" 239; LEOCONOSTOC MESENT " 240; "DEXTRANICUM" 241; "CITROVORUM" 242; BETACOCCUS ARABINOSACEUS "243;" BOVIS "244;" OREMORIS "245;