DE2525841A1 - METHOD OF INHIBITING THE FORMATION OF UNDESIRABLE PRODUCTS ON THE BODY SURFACE - Google Patents

Description

The invention relates to a method for inhibiting the formation of undesirable products on the surface of the body and their closer Environment caused by the action of microorganisms

Lipoid materials are formed in body secretions, as well as agents suitable for this purpose.

The invention is thus directed to methods and means by which undesired problems can be solved that arise caused by the action of microorganisms on body fluids that are secreted by the body.

One of the long standing problems is that of the unpleasant The smell of menstrual fluid. The menstrual fluid contains a variety of substances including

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Proteins and lipids. Normally there are large numbers of "gram-negative and gram-positive" in menstrual fluid Organisms are present that can act on these natural products. It has been recognized that due to exposure an unpleasant amine odor is released by bacteria on proteins. When the bacteria act on lipids Unpleasant-smelling materials can be formed, which are fatty acids.

The underarm odor or the odor of the armpits is also a long-standing problem. The armpit sweat consists of secretions from the apocrine and eccrine sweat glands that are found in the armpit area. Although eccrine sweat is mostly water and salt, apocrine sweat contains a wide variety of substances, including protein, carbohydrates, and lipids. A variety of microorganisms, including staphylococci and corynebacteria, are present on the skin surfaces in the armpit area. It has been shown that the microbiological decomposition of the lipids of apocrine sweat, which leads to the formation of low molecular weight fatty acids, is one of the main reasons for unpleasant smells in the armpit area (Borick et al., Antimicrobial Agents Annual 1960, pages 647-651, Plenum Press , Inc. New York).

Another apparently independent problem is inflammatory skin disorders such as acne. Sebum is a lipid mixture which is secreted by the sebum glands and which contains a variety of fatty acids (Baughton et al., J. Invest, Dermat., J33_ (1959) pages 49-55). It is assumed that free fatty acids formed from the lipids are predominantly responsible for the inflammatory conditions of the skin known as acne vulgaris (Freinkel et al., New'Eng. J. Med. 273 (1965), pages 850-854) . It has been reported that microorganisms, particularly Corynebacterium acne, an organism that is present in both normal and diseased follicles, cause the formation of fatty acids (Scheimann et al.,

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- 3 - 2 5 2 ^r j 8 A 1

J. Invest. Dermat. 3 ± (1960) 171-174).

To combat and relieve the unpleasant odors of body products such as sweat and menstrual fluids unpleasant disorders, like acne, germicides and antibiotics have been used. Hexachlorophene was once a popular ingredient of preparations to combat sweat odor, while antibiotics, such as tetracycline, have been successful in treating it used by acne. However, these results of exposure to germicides and antibiotics were due to a kill of the organisms, which results in a disruption of the normal balance of the microorganisms. How well known is, by killing non-pathogenic organisms, the settlement of opportunistic organisms, such as pathogenic bacteria, Yeasts or fungi favored, the presence of which translates into feverish states, inflammatory states, dermatitis or others can manifest undesirable phenomena. It is therefore desirable combating unwanted body odors or disorders such as acne caused by the action of microorganisms on lipids are caused to cause such that no substantial killing of the non-pathogenic microbial flora takes place. Furthermore, it is desirable to achieve the results without harming the human patient.

Other methods of combating undesirable problems have used products that target the causative agent act after its formation. This method is generally unsatisfactory because it involves the use of relatively large amounts of the treatment agent and / or requires long treatment times. Furthermore, the results are not complete in all cases has been satisfactory both in terms of completeness of control and avoidance of side reactions concerns. It is therefore highly desirable to solve the undesirable problems by preventing the formation of the material, that causes these problems.

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The invention is based on the knowledge that the above-mentioned problems and a number of other undesirable effects are due to microorganisms that act in a similar way, so that the various problems in a similar way can be solved. It has also been shown that this control can be carried out without any substantial killing of most of the microorganisms the bacterial flora can be effected.

It has been shown according to the invention that a number of the undesirable Problems such as menstrual odor, underarm odor and inflammatory conditions such as acne caused by the formation unwanted products are caused on the body surface and its immediate surroundings, and which are caused by the effect are formed by microorganisms on lipoid materials in body secretions, can be solved by the Formation of the undesired products is inhibited by applying an inhibiting effect to the site of formation of the undesired products Amount of an amino acid compound applies.

The term "lipoid materials in body secretions" includes lipids (in the sense of chemical terminology) that are present in body fluids are present, which in turn are deposited, excreted or exuded as secretions. Thus stands the Designation "secretions" also for waste liquids. Typical secretions are sebum, sweat, menstrual fluid, etc.

The lipids which are of importance according to the invention are involved it is not only about triglycerides but also about phospholipids. The triglycerides can be generalized by the following Formula I.

CH ₀ -0-CR I 0 CH-OCR I

CH ₂ -OCR

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are reproduced, where in this and the following formulas the groups R given for similar or stand different hydrocarbon groups derived from fatty acids. The important ones according to the invention Phospholipids are phosphotriglycerides that are represented by the following general formula II

0
CH ₀ -OCR

I 0

CH-O-C-R II

CH ₀ -OPOB ² i
0

can be reproduced, in which R has the meaning given above and B represents the remainder of an alcohol-amine compound, such as an amino alcohol, a quaternary hydroxyammonium base or a hydroxy amino acid.

The products formed or formed by the action of microorganisms on lipoid materials can be similar to those products that can be formed in chemical hydrolysis. Thus, according to the following Equation formed from the triglycerides of the general formula I fatty acids and glycerine:

CH ₂ OH

CHOH + 3 RCOOH

CH ₂ OH

Since the groups R can be different from one another, different fatty acids can be formed. Among the fatty acids, The unpleasant smelling low molecular weight fatty acids such as butyric acid, iso-

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butyric acid, isovaleric acid etc. which cause the problems of underarm odor and menstrual odor. These and other fatty acids, including the higher molecular weight fatty acids (with up to 18 carbon atoms), can be the Reasons for other unwanted problems, such as inflammatory conditions of the skin.

The fatty acids can also be formed from the phospholipids of the general formula II:

CH ₀ OH

II > CHOH + 2 RCOOH + Η-, ΡΟ, + BOH

I.
CH ₂ OH

In the preliminary studies described below it has been shown that phospholipids are a source of unpleasant smelling Represent fatty acids.

It is further recognized that the undesirable fatty acids, as a result of the action of the microorganisms, also appear in other ways can be formed so that control of these fatty acids is also included. It is believed, however, that the above pathways are the main source of fatty acids.

The term "place of education" stands for the place or the place where the lipoid secretions are present and are attacked by the microorganisms.

Usually the most important "point of formation" is the surface of the body or the surface of the skin and its surroundings. This includes the skin, the vagina, and materials that are in close proximity to the surfaces of the body, such as Menstrual pads, items of clothing, bed pillows, etc., which are used to absorb the body fluids, or the record this.

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The amino acid compounds useful in the invention generally have at least one in structure following combinations of an amino acid group with an acid group:

Amino C (C) acid.

0.1

The acid group is preferably a carboxylic acid group (-COOH), although it can also be a sulfonic acid group (-SO ₂ OH) or a phosphonic acid group (-PO (OH) ₂ ). The amino group can be substituted, and the carbon chain can have further groups such as hydroxyl groups (-0H), sulfhydryl groups (-SH) and ether groups (-0-). In general, there will be more than one acid group-containing group in the molecule, which acid group-containing group may be attached to the same amino nitrogen atom. The compounds can be referred to as "aminopolyacid compounds".

An important group of compounds which inhibit the formation of fatty acids are the aminopolycarboxylic acid compounds. The term "aminopolycarboxylic acid compound" includes an amino acid and its water-soluble salts which have two or more Substituent groups of the following structure

(C) COOH

0.1

have in the molecule bound to one or more amino groups. When more than two such substituent groups are present, the excess carboxyl groups can be esterified, provided that at least two of the named Groups are present.

Salts preferred for certain applications are monovalent Salts, such as the sodium salts and the potassium salts. For certain other purposes, the alkanolamine salts are preferred,

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especially those of the di- and trialkanolamines, such as triethanolamine, Diethanolamine, triisopropanolamine, etc. The best known aminopolycarboxylic acid compounds are ethylenediaminetetraacetic acid (EDTA) and its salts, diethylenetriaminepentaacetic acid (DTRA) and its salts and N-hydroxyethylethylenediamine triacetic acid (HEDTA) and their salts. These compounds are commercially available under the names VERSENE ^,

VERSENEX ®, VERSENOL ®, SEQÜESTRENE G9 etc. available. Other typical compounds of this class are triethylenetetraminehexaacetic acid, 1,2-diaminocyclohexane-N, N'-tetraacetic acid, Ν, Ν-dihydroxyethylethylenediaminediacetic acid, iminodiacetic acid, hydroxyethyliminodiacetic acid and the nitrilotriacetic acid and analogous salts thereof.

Other compounds include the N, N'-dihexadecyl ester of Ethylenediaminetetraacetic acid, the Ν, Ν'-dioctadecyl ester of Ethylenediaminetetraacetic acid, the N, N'-di (1 5-carboxypentadecyl) ester the ethylenediaminetetraacetic acid, the N- (15-carboxy-9-pentadecyl) -iminobis- (äthylenenenitrilo) -tetraacetic acid, N-methoxyethyl-iminodiacetic acid, ethylenebis (oxypropylamino-diacetic acid), ethylene bis (oxyäthyliminodiacetic acid), aminoethyl-N-methyliminodiacetic acid, OC-mercaptoethyliminodiacetic acid, the ct-methylmercaptoethyliminodiacetic acid Etc.

Amino acid compounds of other classes that are used for certain purposes Of particular interest are those compounds which also contain more than one acid group Have substituents attached to the amino group, however, their acid groups are generally phosphonic acid groups or sulfonic acid groups. These amino acid compounds however, they can also have mixed acid groups, i. H. one or more carboxyl groups in the same Have molecule which contains the phosphonic acid groups or the sulfonic acid groups. Representative amino acids of these

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Type are ethylenediaminotetra- (methylenephosphonic acid), nitrilotris- (methylenephosphonic acid), Iminobis (methylene sulfonic acid), aminomethylphosphonic acid (Ν, Ν'-diacetic acid) Etc.

The amino acid compounds are available in the form of the free acids, the acid salts and the salts. Preferably turns the amino acid compound or the combination of these compounds in such a form that its pH of the treatment site is close to the neutral range and is about 6.2 to about 8.5. However, it can also be useful Results are achieved when the pH is at least 4.0. It is often convenient to use a salt or an acid salt through it prepare by mixing the acid and base in the preparation.

The amino acid compounds are useful in amounts that provide a final concentration of at least 0.01% by weight based on the Body secretions, surrendered. The upper limit is mainly determined by practical circumstances. In general, there will be no Another advantage achieved by adding an amount that has a concentration of more than about 0.5 wt .-%, based on the Body secretions, results. The preferred amounts depend on the purpose, location, mode of application and the particular connection away. When the microorganism population is large, as in the case of menstrual fluid, are preferred larger quantities used. Thus, to combat menstrual odor, at least about 0.04% by weight, based on the Menstrual fluid, desirable, and 0.10% by weight or more preferred. Those within the broad range are preferred Ranges result from the following description of the special use and the type of application.

The amino acid compound can be applied in various ways to the area where the action of the Microorganisms which produce undesirable products are formed.

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For the purpose of inhibiting the formation of malodorous materials in the menstrual fluid, the amino acid compound is suitably applied to the materials used to absorb the fluid, such as sanitary napkins or other materials that can absorb the fluid, such as bed pillows, clothes, etc. The term "sanitary napkin" or ""Menstrualnapkin" includes sanitary napkins, tampons, and labia pads, usually comprised of a core of one or more layers of highly absorbent, relatively dense materials having a fluid-permeable, soft, knitted, woven or non-woven cover. The cores usually consist of fiber layers, such as carded cotton fleece, air-laid cellulose fiber fleece, wadding made from shredded wood pulp, tissue pulp or similar materials, but can also consist of newer synthetic materials, such as synthetic polymer foams and fibers. Although the amino acid compound can be evenly distributed throughout the sanitary napkin, it is more useful to introduce it into the area that will be the first to come into contact with body fluid. Thus, the material is preferably applied to the surface of the absorbent core in the napkin or the wrapper of the napkin or both materials in such a way that the amino acid compound on the surface in an amount of about 0.0002 to about 0.02 g / cm ² ( 0.001-0.1 g. Per square inch) is available. It has been shown that the desired concentration in terms of the amount of the amino acid compound, based on the total body secretions, is achieved with such amounts. A preferred range for sanitary napkins is from about 0.006 to 0.009 g / cm ² (0.004-0.06 g. Per square inch).

The amino acid compounds can be applied to the sanitary napkins during manufacture or during use. If the material is applied during manufacture, it can be applied by either placing it in In the form of an aqueous spray solution or an aerosol spray mist

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that it is padded, impregnated, or dusted, or with the help of any other Applying methods known for such materials. Propellants such as Use dichlorodifluoromethane or trichlorofluoromethane, while essentially inert solvents such as isopropanol are used to form the solvent spray. The use of a propellant or an inert organic solvent is compared to an aqueous solution or a suspension is preferred to allow the sanitary napkin to dry after the application of the amino acid compound to facilitate. Furthermore, the amino acid compound can be used in the form of dry powder formulations before the bandages are used raise.

One can also apply the amino acid compound topically to the skin surfaces apply, which is preferably done with the help of a suitable carrier material, the material in a applies to the surface of the skin in such an amount that

a concentration of at least 0.01% by weight, based on the body secretions, results. The application is preferably carried out before the leakage of body fluid to the formation of the undesired products essentially completely. However, the application can then also be applied to the The body secretions are released, in which case the action of the microorganisms on the body secretions occurs a minimum is brought. Topical application to the skin surface is both effective in inhibiting the development of undesirable Inflammatory conditions of the skin, as well as for inhibiting the development of bad smells. If that When applied topically, the carrier material can be in solid, liquid, spray or semi-solid form cosmetic or pharmaceutical carrier materials are present which are suitable for topical application. Carrier materials, into which the amino acid compound can be incorporated,

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include lotions, ointments, aerosol spray solutions, aqueous Solutions, creams, powder mixtures, gel pens and the like. A. The various additives and diluents include Ointment additives, such as polysorbate 80 (polyoxyethylene sorbitan monooleate), Polyoxyethylene sorbxtane trioleate; surfactants and emulsifiers such as lauryl sulfate, sodium cetyl sulfate, Glycerine monostearate, diethylaminoethylalkylamide phosphate, Isopropyl myristate, octyl alcohol, glycerin and glycol esters of stearic acids; Glycols such as propylene glycol; other polyhydroxy compounds such as glycerin and sorbitol; Alcohols such as ethanol or isopropanol; Witch hazel water; Fragrances; essential oils; Propellants, such as halogenated hydrocarbons, for example dichlorodifluoromethane, Dichlorofluoroethane, etc., carbon dioxide and nitrogen; solid diluents, such as calcium carbonate, Starch, bentonite and talc; and silicone-like liquids such as polysiloxane liquids. Choosing the special Support materials depend on the intended use. For

Aqueous preparations are desirable for skin preparations for combating inflammatory skin conditions. The preferred preparations for these uses are those that are used as the carrier component Contains ha mamelis water.

In preparations suitable for topical application, the amino acid compound is used in an amount of at least 0.05 % By weight used. This is because of the dilution effect and the possible partial deactivating effect of the carrier material he wishes. The amino acid compound can be present in larger quantities and can even be the main component of the preparation make up, although this is less desirable for practical reasons. For topical purposes The amino acid compounds are preferably used in the form of the alkanolaiain salts. If an aqueous carrier material When used, the salt in the preparation can be formed by adding the free acid and the alkanolamine

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mixed into the aqueous carrier material.

In the case of preparations for combating skin inflammation, the amino acid compounds used are preferably in the form of Acid or a water-soluble monovalent salt or in the form of a mixture in an aqueous preparation in one Amount of from about 2 to about 15 weight percent based on the free acid.

When the amino acid compound is incorporated into a lotion, cream, or aerosol formulation, the amino acid compound can be added in a solvent that is compatible with the system into which it is incorporated, such as water, glycerine, propylene glycol, tripropylene glycol, methyl ether, Ethanol, etc. Alternatively, the amino acid compound can be added to the final formulation and intimately mixed therewith will. This is the preferred method for the production of dusting powders as well as for the production of aqueous solutions, for example those based on witch hazel water.

The preparations described above are applied to the area where the undesirable materials are formed by the various microorganisms. The microorganisms that contribute to the formation of materials that cause the menstrual odor include gram-negative organisms, such as Proteus mirabilis, Klebsiella pneumoniae, Aerobacter aerogenes, Escherichia coli, Pseudomonas aeruginosa etc., gram-positive organisms such as Staphylococcus aureus, Streptococcus faecalis etc. and yeasts such as Candida albicans, etc. The microorganisms that lead to the formation of substances that cause the underarm sweat odor are those that normally occur are present on the skin surface, of which the bacteria of the genera Corynebacterium and Staphylococcus are the most important are. Coryne bacteria are also found where acids are formed that lead to undesirable inflammatory conditions contribute to the skin. By using the inven-

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With the compounds and preparations according to the invention, the action of the microorganisms which leads to the formation of the undesired acid is inhibited or changed in a certain way. It is believed that the amino acid compound acts by removing the necessary metallic cofactor for the enzymatic formation of the fatty acids. The invention is not limited by theoretical considerations, which hold is _f that the elimination of fatty acid production can be achieved without having a deleterious effect on the Mirkobenflora exerted.

The effectiveness of the amino acid compound in inhibiting the formation of unwanted fatty acids from the lipids is made with gas chromatographic analyzes while examining the effectiveness of odor control with the help of organoleptic Methods is assessed.

A comparison is made in the gas chromatographic analysis with known acids, the method used is the following:

The fatty acids are extracted from the acidified test samples with the aid of diethyl ether and determined by gas chromatography using a gas chromatograph (Hewlett-Packard 762OA) equipped with a glass column that is 1.83 m (6 feet) long and has an internal diameter of 2 mm and filled with Porapak QS 80 / particle diameter 0.149 mm (100 mesh) «The instrument is programmed from 135 ° to an upper limit of 235 ° and at a heating rate of 40 C / min and maintains the upper temperature limit for 6 Minutes, Helium is used as the carrier gas at a pressure of 4 _f 22 kg / cm ² (6O psi)
of 40 ml / min. is used.

2
of 4 _f 22 kg / cm (60 psi) and a flow rate

A quantitative organoleptic method is used to evaluate the effectiveness of the sanitary napkins with regard to odor control Evaluation method applied, which is called the organoleptic evaluation method with modified evaluation standard and is carried out as follows:

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Organoleptic evaluation method with modified evaluation scale

This method is designed to be used by an organoleptic Values obtained in the evaluation group enable the sample to be evaluated with an absolute value for the odor intensity. Thus, not only can the difference between two samples of an odorous substance (e.g. on a pillow with or without deodorant), but the rating also gives in quantitatively indicates whether the odor intensities of the samples are strong or weak. For example, a sample can be a contain deodorant which is much more effective than that contained in a second sample. Independently of however, the two deodorants can only cause a slight reduction in odor intensity, which can be assessed using the present assessment method.

The first step in this method is to determine the threshold concentration of the odorous substance. The method used has been described by Fred H. Steiger in Chemical Technology, Volume 1, page 225, April 1971, in relation to the odor threshold concentration for ethylamine and uses the Weibull distribution function. In general, this method is used to determine organoleptic values with the help of a group of test persons who are confronted with a series of samples which contain the odorous substance in increasing concentrations in order to determine the concentration at which an arbitrarily set percentage of test persons can detect the smell. For purposes of the present evaluation, this arbitrary percentage is chosen as a cumulative percentage of 50%. The threshold concentration of the odorous substance determined in this way is specific to the odorous substance and the conditions of sampling and sample presentation.

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The method used according to the invention consists in to provide each subject with a series of samples in a sample device that has an opaque 0.5 1 (1 pint) polyethylene bottle according to Mason with polyethylene screw cap. The bottle is inside lined with a polyethylene bag and provided with a Büchner funnel on the lid, the one below the filter plate lying narrow outlet extends through the cap and into the lined bottle. Then a sample is put into the The bottle was brought in, the bottle was closed with the Buchner funnel, after which a watch glass was placed on the wide inlet area the funnel is placed. The sample is then allowed to equilibrate under room conditions for one hour. To determine the threshold concentration, samples are used which contain an aqueous solution of the fragrance with a specific Contains fragrance concentration, a total of 3 ml of the solution are introduced into the bottle. Then poses provide a group of about 30 women with a series of balanced samples of increasing concentration and encourages the test subjects to do so, starting with the sample with a concentration of zero, which only contains water, indicate the first sample that has a detectable odor. The subjects are then instructed to smell each sample in turn, with between each sample and a pause of 30 seconds is switched on for the next sample. The values obtained are used to calculate the cumulative Determine the percentage of subjects who, at each concentration value corresponding to each sample, detects an odor. The values determined in this way are compared to that described in the above-mentioned article by Steiger Weibull probability paper plotted by putting the concentration on the abscissa and the cumulative Plots the percentage of the test group on the ordinate. The concentration at 50% is then used as the threshold concentration taken.

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After the threshold value has been determined, the method with the modified evaluation standard is applied by creating a calibration curve. Using the same test device, a series of samples are prepared and made available to the experimental group that contain the odorant in concentrations equal to multiples of the threshold concentration or odor units. One of these samples contains 20 times the threshold concentration (20 odor units). According to the standard method of ratio evaluation, each subject is asked to evaluate the group of samples available to him and to assign each sample a value for the odor intensity in relation to the intensity of the other samples. The test subjects can use any standard. For example, a test person can assign the assessment number 10 to the strongest sample. Evaluation number 5 is then assigned to a sample which has half the intensity in the evaluation of this test person. The values determined then comprise a series of evaluation numbers for each test subject, each series being based on the test subject's individual yardstick. The sample with a concentration that corresponds to 20 times the threshold concentration is then randomly _{assigned a rating number of} 100. Then the evaluations of each individual test person are converted in such a way that the basis 100 for the 20-fold threshold concentration results for the individual standards. For example, the evaluation of the test person who has given a first sample with a concentration that corresponds to 20 times the threshold concentration, the evaluation number 10, converted such that the first sample receives a value of 100 and the second sample a value of 50. The values are then grouped in such a way that for each sample that corresponds to a specific multiple of the threshold concentration, there is a specific evaluation number (20 times the threshold concentration = 100), which is based on the same standard for all test subjects and that of the evaluation of the Test subject corresponds. Then the geometric mean of the ratio values of each sample is calculated, and the value as the ratio value for that multiple

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- 18 of the threshold concentration defined.

Taking the logarithm of the ratio on the ordinate versus the logarithm of the multiple of the threshold concentration plots, a straight line attached to the points of 3 times to 20 times the threshold concentration gives a excellent correlation.

It has been shown that regardless of the amine odor substance investigated when determining the threshold concentration for the particular odorous substance and determining the calibration curve in the above manner calibration curves are obtained which are between multiples of the threshold concentration from about 3 to about 20 can be placed on top of each other.

The curve determined in this way for isobutyric acid, a chemically dissimilar material, is also consistent with the calibration curves of the amines. Thus, the comparative odorant used in any test must match that tested Odor constituent or the odor constituents examined are not chemically similar.

The calibration curve can now be used to evaluate the odor intensity of any odorant when it is introduced into any environment, for example on a pillow made of untreated cellulose fibers or a pillow made of fibers containing a deodorant material, so that in addition to a comparison of the relative intensities of the samples examined an absolute measure of the intensity of each sample can be obtained. For this purpose, the experimental group is provided with a series of samples, including a standard sample which contains the odorous substance examined in a known concentration in an environment which is identical to the environment used to determine the calibration curve. This standard sample preferably has 20 times the threshold concentration and thus a ratio of 100 on the calibration curve.

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Subjects are again asked to rate the set of samples using any standard. On the basis of the value that each test person assigns to the intensity of the standard sample, all other values of the test persons are converted accordingly so that they are consistent with the evaluation of the standard sample. For example, the values of a test person who assigned the evaluation number 50 to the standard sample and the evaluation number 5 to a second sample of unknown intensity are converted in such a way that the standard receives a ratio of 100 and the unknown sample a ratio of 10. By comparison with the calibration curve, it can be determined that the ratio value 10 of the second sample determined by the test person is equivalent to an odor intensity of a certain multiple of the threshold concentration for which 1.2 can be read from the calibration curve, which means that the odor intensity of the sample is unknown Intensity in the test environment used is equal to the intensity of a sample which contains the odorous substance in a concentration which corresponds to 1.2 times the threshold concentration in the standard environment.

Preliminary investigations are carried out on the smell of the menstrual fluid to determine the presence of phospholipids in the menstrual fluid and the formation of bad-smelling fatty acids by the normally microbial flora present in menstrual fluid to prove.

The presence of substantial amounts of phospholipids in the menstrual fluid is determined using a method that consists in treating the phospholipids with perchloric acid heated to oxidize the organic part of the molecule and convert the phosphorus into a blue phosphorus molybdate which can be determined colorimetrically. A detailed description this procedure can be found on pages 375 to 376 of

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"Bray's Clinical Laboratory Methods," by Bauer et al., 17th Edition, CW. Mosby Co., 1969. 10 samples of menstrual fluid are collected in beakers and the Subjected to phospholipid analyzes. The analyzes show the presence of phospholipids in an amount from 2250 to 4140 ppm, which corresponds to 0.225 to 0.4% of the liquid.

To determine the ability of the normally present in the menstrual fluid Existing microorganisms ^ forming malodorous fatty acids are separated from sterile samples Inoculated human blood with various organisms previously isolated from the menstrual fluid. Afterward the material is incubated and gas chromatographic analysis is carried out in the manner described below. In the Provision is used instead of menstrual fluid blood, as sterile blood is available and a sterile substrate for identifying fatty acid formation is desirable, caused by the microorganism with which the blood was inoculated. The suitability of the blood as a substitute is based on the known fact that triglycerides are present in both blood and menstrual fluid, whereby Preliminary tests on six samples of human blood and menstrual fluid have shown that these materials contain an average of 2807 ppm or 2771 ppm total phospholipids. The methods used and those obtained Results are the following:

In each case 2.5 ml of test samples of sterile whole human blood are inoculated with 0.2 ml of a suspension of bacterial cells from various test organisms which have previously been isolated from the menstrual fluid in the usual way. The test samples as well as the nic ^ tanokulierte control sample are incubated for 24 hours at 37 ⁰ C in a constant temperature bath with constant shaking at 200 rpm. All samples are then analyzed by gas analysis. The results obtained are summarized in Table A below.

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- 21 Table A

fatty acid present (ppm)

Isobutyric acid butyric acid isovaleric

acid

59.5 9 111, 7th 0 0 11.3 0 8.7 15.0 0 0 0 0 0 0

uninoculated control 0 0 3.5

gram-negative bacteria Proteus mirabilis Klebsiella pneumoniae Aerobacter aerogenes Escherichia coli Pseudononas aeruginosa

gram positive bacteria

Staphylococcus aureus 13.7 0 50.5

Streptococcus faecalis 0 0 39.1

yeast

Candida albicans 12 0 38.5

The following examples serve to further illustrate the invention.

example 1

Two samples of menstrual fluid are collected, after which the untreated and non-incubated liquid was first examined for fatty acids by gas chromatography isobutyric acid, butyric acid and isovaleric acid. Then test samples, each about 2 ml of the each containing menstrual fluid. Then two groups, each with two different samples the menstrual fluid contain, treated as follows: Disodium ethylenediamine tetraacetate is added to one group up to a final concentration of 0.2% by weight, based on the menstrual fluid, while the other group

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leaves untreated. The treated and the untreated samples are then incubated for 24 hours at 37 ° C. and subsequently analyzed by gas chromatography for the above fatty acids. The results obtained are in the Table I below compiled.

Table I.

fresh, non-incubated Fatty acids (ppm) Butyric acid Isovalerians te, untreated con Isobutyric acid Per troll test be 1 incubated, untreated 36 0 incubated, Na ₂ EDTA be 0 1014.0 857.1 acts 685.7 fresh, non-incubated 16.6 14.3 te, untreated con 0 Per troll test be 2 incubated, untreated 0 0 0 307.0 336.0 475.0

incubated with Na ₂ EDTA

treated 4.0 13.0 9.0

Example 2

Five samples, each containing 2.5 ml of sterile human blood, are inoculated with 0.2 ml of a suspension of Proteus mirabilis. Disodium ethylenediamine tetraacetate is added to four of the samples in amounts corresponding to 0.04 to 0.2%. One sample is an inoculated control sample that does not contain an ethylenediaminetetraacetic acid compound. In addition, a non-inoculated control sample is prepared containing 2.5 ml of the blood to which 0.2 ml of sterile distilled water has been added. The treated and untreated inoculated samples and the control sample are then nichtinokulierte for 24 hours at 37 ⁰ C in a water bath

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incubated at constant temperature, taking the samples shakes at 200 rpm. At the end of the treatment period, aliquots are gas chromatographed for their presence studied by acids. The results obtained are given in Table II below.

Table II

Treatment of fatty acids (ppm)

% Na "EDTA isobutyric acid isovaleric acid

Proteus + no Na ₂ EOTA 102.7 98.2

Proteus + 0.04% Na ₃ EDTA 28.4 52.3

Proteus + 0.1% Na ₂ EDTA 6.6 17.5

Proteus + 0.2% Na ₂ EDTA 7.9 17.6

untreated sterile blood 0 0

Example 3

Sterile blood inoculated with Proteus mirabilis is applied to treated blood and untreated samples of downy pillows and sanitary napkins. Contributes to the preparation of the treated samples one first a 14% aqueous solution of a mixture of disodium ethylenediamine tetraacetate and tetrasodium ethylenediamine tetraacetate (pH value = 7) at different points and in different concentrations on samples from downy pillows or sanitary napkin samples, which is accomplished by impregnating the cover with the material that Material sprayed or padded on. The samples are then dried and it is the amount dispersed in the samples Ethylenediamine tetraacetate salts determined by weighing. Then the blood previously inoculated with Proteus mirabilis is applied the various treated samples as well as an untreated sample and a sample containing dimethylamine as a reference fragrance contains, applied. (Use 3 ml for the down pillows and 5 ml for the sanitary napkins). Samples are then incubated for 24 hours at 37 ° C and

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finally submitted to the organoleptic examination in the manner described above. The results show a significant Reduction of the odor, which can be seen from the following Table III.

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Table III

1 4

percentage odor reduction with different applications of EDTA

III IV V VI

Nonwoven cover cotton fabric on the upper on the embossed

on the monthly as a filter over the surface of the th area of the

tie. the fluff pillow fluff sanitary napkin

Concentration of 0 / 0OQ5 ² I. II EDTA g / g of the un 0.0016
0.0050 in the ge Paper as investigated Inoku- 0.0060
0.0084 velvet fluff Filter over lums 0.0100 dispersed the fluff 0.0140 pillow 0.0200 __3 46 60S 0.0330 «■ M 881, mm at mm mm O 68 O - —— - -

52

46
50

24

42
40

58

Compared to the untreated control sample, as an odor observed.

Estimated based on the concentration of the EDTA solution that was sprayed during manufacture

of the fabric has been applied. to

cn ro cn oo

The dashed lines mean that kexne investigation

was carried out.

The methods used to disperse EDTA are:

1) soaking in a 14% aqueous solution, 2) spraying with an aqueous solution, 3) padding (insert into the solution and then squeeze off with the help of rollers) ; A) padding, 5) spraying with an aqueous solution, 6) spraying with an aqueous solution.

The neutral (pH = 7) ethylenediaminetetraacetate solution is obtained by dissolving 10.0 g of tetrasodium ethylenediamine tetraacetate and 11.4 g of disodium ethylenediamine tetraacetate in 125 ml of distilled water. The formed solution has a concentration of about 14% by weight.

Example 4

In a study lasting over six months, a group of seven test persons was used who wore the sanitary napkins for a minimum of 4 hours or until the point in time that the absorbed menstrual fluid made it necessary to change the sanitary napkin. Within 30 minutes of changing the sanitary napkin, the sanitary napkins are examined and physical characteristics such as weight, physical appearance and stain area are determined. The bandages are then incubated for 1 hour at 30 ^° C. and then examined for their odor.

This procedure is carried out by each test subject with a treated and an untreated (control) sanitary napkin, the used on alternate months. In some cases the values are kept for the entire duration of determined over a period of six months, while in other cases values are only available for 2 months or for 4 months. The selection of a product for a specific test

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person is statistical so that factors such as climate or changes in activity have a minimal influence.

The sanitary napkins used have been produced by removing the non-woven fabric cover of a commercially available sanitary napkin (MODESS Sanitary Napkin from JOHNSON & JOHNSON), guiding the absorbent fabric of the inner filling through a solution containing a mixture of disodium ethylenediamine tetraacetate and tetrasodium contains ethylenediaitiin tetraacetate (and which has been prepared according to Example 3), the material dries and the liquid-permeable shell reattached in place. The concentration of the salt after drying is 0.0006 g / cm ² (0.004 g. Per square inch) of the fabric.

The odor values of all test subjects who were used during this period are collected and the odor intensity of the test products is compared with that of a control sample. The odor intensity of 25 treated and 25 untreated sanitary napkins is shown using odor units ranging from 0 to 21 (maximum). The untreated sanitary napkins had odor units from 3 to 21, with five sanitary napkins having odor units above 15. Ten of the treated sanitary napkins show odor units less than 3 _# and there are no treated sanitary napkins with odor units greater than 11.

Example 5

2 ml samples of diluted human plasma (1 part plasma and 2 parts sterile distilled water) are inoculated with Corynebacterium. Disodium ethylenediamine tetraacetate is added to three of the samples in such an amount that concentrations of 0.1 , 0.2 and 0.5% result. A sample containing no sodium ethylenediaminetetraacetate serves as an untreated control sample. In addition, a non-inoculated control sample is used

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unmodified diluted human plasma. the Test samples and control samples are incubated for 24 hours at 37 ° C, shaking at about 200 rpm. After the time has elapsed, the samples are examined for the content of free fatty acids by gas chromatography. The results obtained are the following:

Table IV

Treatment of fatty acids (ppm)

Isobutyric acid isovaleric acid

untreated sterile plasma 0 0

Corynebacterium inoculum

- no Na ₃ EDTA 155 564

Corynebacterium inoculum

+ 0.5% Na ₂ EDTA 12 22

Corynebacterium inoculum

+ 0.2% Na ₂ EDTA 25 49

Corynebacterium inoculum

+ 0.1% Na ₂ EDTA 22 41

Example 6

To 2 ml of a brain-heart infusion broth (BHI), which the microorganisms contains the bacterial flora found in the armpit, mainly Corynebacterium and Staphylococcus includes, disodium ethylenediamine tetraacetate is added to a final concentration of 0.5% by weight. Another sample of Disodium ethylenediaminetetraacetate is not added to 2 ml of the inoculated broth. In addition, one uses a 2 ml sample of the sterile brain-heart infusion broth as a non-inoculated control sample. The test sample and the control sample are incubated for 24 hours at 37 ° C with shaking at 200 rpm. After the time has elapsed, the samples are

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examined chromatographically with respect to the free fatty acids. The results obtained are summarized in Table V below.

Table V

Treatment of fatty acids (ppm)

Isobutyric acid isovaleric acid

Sterile BHI broth 0 0

BHI broth with the forearm bacterial flora inoculated 32 57

BHI broth inoculated with the forearm flora
+ 0.5% Na EDTA 0 0

Example 7

An aqueous solution containing 0.45 percent by weight of a mixture of Na "EDTA and Na ₄ EDTA (and which has been prepared according to Example 3) is applied to an armpit with the aid of a sterile gauze pad that has been wetted with the solution Test person on. Sterile distilled water is applied in the same way to the other armpit, which serves as an untreated control. Then both armpits are examined by smelling twice a day to determine the odor development and the odor intensity by comparison. The treated armpit level shows a very substantial deodorized effect.

Example 8

A be applied for the treatment of inflammatory conditions of the skin on the body cream is prepared by (T) heating the specified hereinafter ingredient A at 70 ⁰ C, (2) heating the specified in the following component B at 75 ° C, (3) adding the Component B with stirring to component A and (4) adjusting the pH value with dilute sodium hydroxide

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solution to 5.5.

Component A% by weight

Cetyl alcohol 2.5

Stearyl alcohol 5.0

Isopropyl myristate 2.0

light silicone oil 1.0

Sodium salt of the reaction product
of lactic acid and stearic acid 1.5

(Eraplex, Patco Products, Kansas City,
Missouri)
Methyl hydroxybenzoate 0.15

(Methyl paraben)

Propyl hydroxybenzoate 0.05

(Propyl paraben)

Component B

deionized water 78.8

Disodium salt of EDTA 4.0

Propylene glycol 5.0

Example 9

A lotion suitable as a skin lotion is prepared by heating

(1) of the component A described below to 72 ⁰ C,

(2) heating of component B indicated below to 75 ° C,

(3) Adding the component B to the component with stirring A and

(4) Adjust the pH to 5.3 with dilute sodium hydroxide solution.

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Component A Wt% Cetyl alcohol -1.9 Stearyl alcohol 3.0 Isopropyl myristate 1.3 light silicone oil 0.8 Sodium salt of the reaction product of lactic acid and Stearic acid 1.1 (Emplex, Patco Procucts, Kansas City, Missouri) Methyl hydroxybenzoate 0.15 (Methyl paraben) Propyl hydroxybenzoate 0.05 (Propyl paraben)

Component B

deionized water 81.7

Propylene glycol 3.0

Disodium salt of EDTA 7.0

Example 10

The preparations described in Examples 8 and 9 can be applied to the skin surfaces in order to To prevent the formation of fatty acids, which are formed by the action of corynebacteria on the leaked sebum, because these fatty acids are undesirable in skin inflammation.

Example 11

One prepares a suitable odor control hand and body lotion by (1) heating the specified hereinafter ingredient A at 82 ° C, (2) heating the specified in the following component B at 78 ⁰ C, (3) adding the component ( A) with stirring to the component B and (4) cooling down

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- 32 46 ⁰ C and adding component C.

Component A% by weight

Mineral oil 3.00

Glyceryl monostearate 5.00

Isopropyl Palmitate 3.00 Sterolatum (Amerchol-H-9, Amerchol

Products, Ine.) 1.00

Stearic acid 1.50

Propyl hydroxybenzoate (propyl paraben) 0.05

Component B

Methyl hydroxybenzoate (methyl paraben) 0.15

2-Bromo-2-nitropropane-1,3-diol (Onyxide 500 0.20

the Onyx Co.)

Propylene Glycol - USP 4.00

Glycerin 96% - USP '3.00

Sulphosuccinate half ester (Standapol SHC-301, 2.50

Henkel Co.)

Disodium salt of EDTA 5.0

deionized water 71.35

Component C

Fragrance 0.25

Example 12

A body powder suitable for topical application is made by vigorously mixing the following ingredients from:

Trisodium ethylenediamine tetraacetate 10 g

Talc 787 g

Fragrance 3 g

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Example 13

The preparation described in Example 12 can be applied to the surface of the skin applied to the formation of bad smelling fatty acids due to the action of Coryne bacteria to prevent the deposited sebum.

Example 14

One prepares a sanitary napkin having an absorbent core of comminuted wood pulp and a porous nonwoven fabric sheath, one on the upper surface of the wood pulp core Natriumnxtrilotriacetat mg in an amount of 0.155 per cm ² of the surface (1.0 mg per square inch) applies. During production, the nitrilotriacetate is applied in a dry state with the aid of an aerosol spray solution containing dichlorodifluoromethane as a propellant onto the absorbent core, which is then surrounded by the nonwoven cover.

Example 15

A sanitary napkin is prepared similar to that described in Example 14, with the difference that both the upper surface of the wood pulp core and the liquid-permeable cover with sodium N-hydroxyethylethylenediamine triacetate (HEDTA-SaIz) in an amount of 0.248 mg per cm ^{2 of} the surface ( 1.6 mg. Per square inch).

Example 16

A menstrual tampon is prepared which has an absorbent compressed cylindrical core of fabric and short rayon fibers. A neutral mixture of disodium ethylenediamine tetraacetate and tetrasodium ethylenediamine tetraacetate in an amount of 0.233 mg / cm ² (1.5 mg. Per square inch) is applied to the front half of the surface of the core. The salt is used in a dry state

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Using an aerosol spray solution containing dichlorodifluoromethane as Containing propellant applied to the surface of the core. The core is then wrapped in a non-woven fabric cover and fastened at the front end a strap for pulling out the tampon by tying it.

Example 17

A tampon similar to the menstrual tampon described in Example 16 is made up with 0.155 mg / cm ² (1.0 mg. Per square inch) trisodium ethylenediamine tetraacetate on 2/3 of the front surface of the absorbent core and the liquid-permeable cover.

Example 18

By mixing the following materials in the stated proportions at about 60 ⁰ C to prepare a cream pin:

Components Weight in g

Ozokerite wax 48.0

Carnauba wax 32.0

Candellila wax 64.0

alkoxylated alcohol 42.0 (Emcol 249-3K, Witco Chemical Company)

a mixture of 20% butylated hydroxy

anisole, 20% butylated hydroxytoluene and

60% corn oil (Tenox 4 from Eastman Chemical

Products Inc.) 0.8

modified bentonite (Benton M-20, National 80.0 Lead Company)

Talc 120.0

Propyl hydroxybenzoate (propyl paraben) 0.8

neutral mixture of Na ₃ EDTA + Na ₄ EDTA 40.0 (for preparation see footnote 5) of Table III)

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The mixture is then poured into cooled molds and receives a stick-shaped end product through cooling.

Then test subjects are given two code numbers Provided cream sticks to be used as armpit deodorants, one stick having the above composition and the other stick has a similar composition, but not the mixture of the two sodium ethylenediaminetetraacetate salts contains. The test subjects are not informed which pen contains the EDTA salts.

The test subjects treat one armpit with one Pen and the other armpit level with the other pen, whereupon qualitative assessments of odor intensity are made. The test results are summarized in Table VI below.

Table VI

, Odor intensity through self-assessment experimental _

"Control armpit examination armpit

cave

A strong 'no odor

B very strong no odor

C very strong no odor

D strong no odor

E strong no odor

F very strong low odor

G very strong low odor

H strong low odor

I very strong, low odor

J very strong low odor

K strong low odor

L strong low odor

M - very strong low odor

Example 19

Man preparing an aerosol formulation by first mixing the following materials in the proportions specified:

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Parts by weight

Micropulverized Talc 2.50

Micropowdered Na ₃ EDTA 2.50

Fragrance 0.16

anhydrous ethanol 0.20

Isopropyl myristate 0.60

Then you bring the mixture into pressure vessels (spray cans) and use the following propellants in the specified proportions:

Trichloromonofluoromethane (Freon 11,

EGG. duPont de Nemours & Co.) 47.02

Dichlorodifluoromethane (Freon 12,

EGG. duPont de Nemours & Co.) 47.20

Example 20

In an experiment carried out in a manner similar to that described in Example 5, test samples are prepared by adding one of the following aminopolycarboxylic acid compounds to test samples inoculated with Corynebacterium from sterile plasma: disodium ethylenediamine tetraacetate (Na ₂ EDTA), N-hydroxyethylethylenediamine triacetic acid ( HEDTA), diethylenetriaminepentaacetic acid (DTPA), ethylene glycol bis (aminoethyl ether) tetraacetic acid (EGTA) and a triethanolamine salt of ethylenediaminetetraacetic acid (TEDTA), which is obtained by adding triethanolamine to an aqueous ethylenediamine tetraacetic acid of up to 6.3. The amount in which the aminopolycarboxylic acid compound is added to the samples is so great that the test medium contains 0.2% by weight of the aminopolycarboxylic acid compound (calculated as free acid). In addition, two control samples are prepared, one from unmodified sterile plasma (non-inoculated and containing no aminopolycarboxylic acid compound) and a second control sample, which is an inoculated sample that does not contain amino-

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2525341

contains polycarboxylic acid compound and serves as an untreated control. The samples are incubated and as previously described investigated in relation to the free fatty acids. The results obtained are the following:

Table VII

Fatty acids formed (ppm) isobutyric acid isovaleric acid

untreated sterile

Plasma 0 0

Corynebacterium inoculum
no aminopolycarboxylic acid compound 105

Corynebacterium inoculum

+ 0.1 % Na ₂ EDTA 0 0

Corynebacterium inoculum

+ 0.1 % HEDTA 0 0

Corynebacterium inoculum

+ 0.1 % DTPA 0 0

Corynebacterium inoculum

+ 0.1 % EGTA 0 0

Corynebacterium inoculum

+ 0.1% TEDTA 0 0

Example 21

One prepares sanitary napkins, which are impregnated on the upper surface of the absorbent core and the liquid-permeable casing with a Amxnopolycarbonsäureverbindung in a similar manner as in Examples 14 and 15, with the difference that they used the Amxnopolycarbonsäureverbindung and per cm ² of the surface with respect to Quantity are modified as follows:

Disodium ethylene glycol bis (aminoethyl ether) tetraacetate, 0.465 mg / cm ² (3 mg. Per square inch),

Sodium iminodiacetate, 0.310 mg / cm ² (2 mg. Per square inch),

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Ethylenediaminetetra (methylenephosphonic acid) neutralized with diethanolamine to a pH of 6.3, 0.233 mg / cm ² (1.5 mg. Per square inch),

Pentasodium nitrilotris (methylene phosphonate), 0.310 mg / cm ² (2 mg. Per square inch),

Ethylenediamine tetra (methyl sulfonic acid) neutralized with triethanolamine to pH 6.3, 0.310 mg / cm ² (2 mg. Per square inch).

Example 22

Preparations are carried out in the manner described in Example 18 by mixing the following materials in the proportions given a cream stick:

Component weight in grams

Ozokerite wax 48.0

Carnauba wax 32.0

Candellila wax 64.0

alkoxylated alcohol 42.0 (Emcol 249-3K, Witco Chemical Co.)

Mixture of 20% butylated hydroxy

anisole, 20% butylated hydroxytoluene

and 60% corn oil (Tenox 4, Eastman 0.8

Chemical Products Inc.)

modified bentonite (Benton M-20, 80.0 National Lead Co.)

Talc 120.0

Propyl hydroxybenzoate (propyl paraben) 0.8 triethanolamine salt of the ethylenediamine

tetraacetic acid 50.0

The pencil can be applied to the skin surface of the armpit to prevent the formation of odorous fatty acids due to the action of Corynebacterium on lipoid materials to prevent.

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Example 23

By intimately mixing the ingredients given below Preparations for topical application with which fatty acid formation can be produced in the specified amounts from lipoid materials can be inhibited by the Coryne bacteria.

Preparation A parts by weight

N-hydroxyethylethylenediamine

tetraacetic acid 10

Triethanolamine 10

Witch hazel water 80

Preparation B

Ethylene glycol bis (aminoethyl ether) -

tetraacetic acid 13

Triethanolamine 13.8

Witch Hazel 73.2

Preparation C

Ethylenediaminetetraacetic acid 10

Triisopropanolamine 17.7

Witch hazel water 72.3

Example 24

A preparation suitable for application to the skin tissue is prepared by intimately mixing the following ingredients :

Ethylenediaminetetraacetic acid 10 parts by weight

Triethanolamine 13.8 parts by weight

Witch hazel water 76.2 parts by weight

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This preparation can be applied to the skin tissue to inhibit the formation of inflammatory skin conditions, caused by fatty acids extracted from the excreted lipoid materials by the action of Coryne bacteria are formed. For use, it is applied daily by rubbing the affected area of the skin surface and repeating this order until the condition is alleviated. Further applications can be made on the sites occur at which the undesirable conditions develop in order to prevent further development of these conditions.

Example 25

The formulation of Example 24 is used to determine the effectiveness of inhibiting undesirable inflammatory conditions the skin typical of acne. The undesirable conditions are those that are considered to be the presence of erythema and the presence of pustules and pustules can be described.

The study examines six subjects with a similar level of inflammation on both sides of the Facial used, after determining the number of erythema-like scars and pustules on each side of the face and which are referred to herein as "infested sites", applies the preparation described above to one half of the face, while the other half of the face serves as a control sample. The applications are made twice a day on one Half of the face after first cleaning both sides of the face separately. The application lasts for 3 weeks continued. After this time has elapsed, the percentage change in the number of infected areas on the treated and the untreated half of the face is determined. The results are as follows:

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Test subject

- 41 Table VII

percentage change treated untreated

1 7o % Reduction 6th % Reduction 2 70 % Reduction 55 % Increase 3 52 % Reduction 7th % Increase 4th 62 % Reduction 20th % Increase 5 62 % Reduction 3 % Reduction 6th 59 % Reduction 5 % Reduction

Although in the past the undesired products formed by the action of the microorganisms on the body secretions were inhibited by killing the microorganisms, it has now been found that the desired inhibiting effect can be achieved according to the invention without the necessary killing of the organisms. This results, for example, from the fact that when Proteus mirabilis is grown in blood mixed with heparin at 37 ^° C. in the presence or absence of disodium ethylenediamine tetraacetate for 24 hours, no harmful effect on the growth of the organisms can be determined, which can be seen from the following table:

Blood inoculated with Proteus mirabilis

no Na ₃ EDTA 2% Na ₂ EDTA

Bacteria count

0 hours

100 χ 10
100 χ 10 '

24 hours

182 χ 10 231 χ 10

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Claims (19)

Claims f'1y methods of inhibiting the formation of undesirable products on the body surface and its immediate surroundings caused by the action of microorganisms on lipoid materials are formed in body secretions, characterized in that one on the point of formation of the undesired products applies an inhibiting amount of an amino acid compound. 2. The method according to claim 1, characterized in that the amino acid compound is an aminopolycarboxylic acid compound begins. 3. The method according to claim 2, characterized in that there is an ethylenediaminetetraacetic acid compound as the aminopolycarboxylic acid compound begins. 4. The method according to claim 1, characterized in that one the amino acid compound is used in an amount such that a final concentration of at least about 0.01 is obtained % By weight, based on the body secretion. 5. The method according to claim 1, characterized in that undesirable Products are combated by the action of gram-negative bacteria and yeasts of the genus Candida the lipoid materials are formed. 6. The method according to claim 1, characterized in that undesirable Products are combated by the action of microorganisms of the genus Corynebacterium and Staphylococcus are formed on the lipoid materials. 7. The method according to claim 1, characterized in that undesirable Products are treated that have an unpleasant odor. 509881/1100 8. The method according to claim 1, characterized in that the formation of undesired free fatty acids is inhibited which lead to undesirable inflammation of the skin. 9. Method of treating skin tissue to help develop to inhibit undesirable inflammatory conditions of the skin caused by free fatty acids, the formed by the action of bacteria on excreted lipoid materials, characterized in that that an inhibiting preparation containing an amino acid compound is applied to the tissue. 10. The method according to claim 9, characterized in that as Amino acid compound an aminopolycarboxylic acid compound is present .. 11. The method according to claim 10, characterized in that the aminopolycarboxylic acid compound is an alkanolamine salt of ethylenediaminetetraacetic acid is available. 12. The method according to claim 9, characterized in that a preparation is used as the inhibiting preparation which contains an amino acid compound in the form of an intimate mixture with witch hazel water. 13. Absorbent sanitary napkin comprising an absorbent core and a liquid-permeable cover arranged around the core, characterized in that the sanitary napkin at least on those areas of the surface which first come into contact with the menstrual fluid during use contains an amino acid compound in an amount of contains at least about 0.0002 g / cm ² . 14. A bandage according to claim 13, characterized in that it contains an aminopolycarboxylic acid compound as the amino acid compound. 509881/1100 15. A bandage according to claim 14, characterized in that it is an ethylenediamine as the aminopolycarboxylic acid compound ■ contains tetraacetic acid compound. 16. A bandage according to claim 13, characterized in that it contains the amino acid compound in an amount of about 0.0006 to about 0.0155 g / cm ² . 17. A preparation which can be applied to the skin tissue to inhibit the formation of undesired microorganisms Fatty acids, characterized in that they contain an amino acid compound in an amount of at least 2% by weight and contains a carrier material suitable for topical application. 18. Preparation according to claim 17, characterized in that it contains an aminopolycarboxylic acid compound as an amino acid compound. 19. Preparation according to claim 17, characterized in that it is an alkanolamine salt of ethylenediaminetetraacetic acid as the amino acid compound and an aqueous preparation containing witch hazel as a carrier material. 50 9 8 8 1/110