DE2442149A1 - Disposable blood-taking vessel - is for extracting centrifuging and separating blood residues and serum and distributing the latter - Google Patents

Abstract

Complete system for taking blood and separating and distributing serum comprises a syringe cylinder (4) with a closable needle attachment (5), a valve insert, and a closure plug. The syringe can be placed in a centrifuge and lcosed at its lower end, either by a stopper in an olive-shaped recess (6) or by an insertable tilt valve. By means of the tilt valve or a slide valve and a sealing ring or a plate valve the blood residues and serum can be completely separated so that material exchange between the two phases is no longer possible.

Description

Title: Disposable vessel for blood collection, centrifugation and subsequent Sample distribution.

Field of application: The invention relates to a complete system for Blood sampling, centrifugation, subsequent separation of blood cake and serum, and for serum distribution in medical and biological laboratories.

Purpose: The present invention aims to obtain a blood sample of from the decrease in the patient to the distribution of the serum at the workplace in the laboratory remains in a jar.

Prior art: In general, blood is injected with a syringe The patient is removed and then placed in a standardized centrifuge tube made of glass or injection-molded plastic. The centrifuge tube is closed with a stopper. In this tube it is now transported to the chemical lab, where it is transferred to a Centrifuge is used to remove the serum (in which most of the analyzes are carried out to win. After centrifugation, the serum: (or plasma if added of anticoagulant substances) poured or pipetted off the blood cake. It must therefore be transferred to another vessel, since the serum does not last a long time may be kept over the blood cake.

Newer developments such as the Monovette or Vacutainer combine a dispensing syringe and centrifuge tubes in a jar. There is also separation in these systems of the serum from the blood cake by transferring the serum into an older vessel.

The acceptance technology associated with the Vacutainer and the Monovette has only been able to assert itself with a small part of the medical profession. Hence based the present invention based on the principle of the conventional syringe so modified was that it can be used in a centrifuge and thereby equilateral serves as a centrifuge tube.

After centrifugation, a valve is placed between the serum and plut cake inserted. This completely separates the serum and prevents it from tipping over the syringe a subsequent mixing of the two plrases.

Description of the present invention: The complete system exists made of 5 plastic parts, which are mainly intended for single use: 1. Syringe sleeve 2. Syringe plunger 3. Sealing plug 4. Valve insert 5. Sealing plunger needle hub, The syringe length and diameter of the syringe barrel have standard dimensions; the plugged The syringe is dimensioned so that it can be used in commercially available centrifuges can.

The syringe cylinder is explained in more detail using two exemplary embodiments: 1st example (see Figure 1): The cylinder (1) shown in cross section has 3 characteristic nerves: 1. Slightly protruding ring on the inner surface about Ics below the upper opening (2).

2. 4 ribs (3) between the bottom of the syringe barrel (4) and the needle hub (5); These ribs give the needle hub a special hold so that it can centrifugation does not stop.

3. The needle hub has an olive-shaped jump on the inside (6) for receiving a sealing plug (9).

4. Closure plug (9) with a thin band (7;) on the syringe barrel hangs, and which is used to close the needle hub.

The stopper (8) in Figure 1 is used to close the syringe barrel during transport from the sampling point to the laboratory.

2nd example (Figure 2): The 2nd application example differs from the first in the different design of the lower pole.

The needle hub is designed as a toggle valve (1). This valve is inserted into the lower pole of the syringe barrel (2). In the on picture 2 the tilt valve is open. It is moved in the direction of the arrow it will be closed. At (3) is a cross section through the injection cylinder on one a little further out, the @ arrow at (1) accordingly presented @ t.

An exemplary embodiment of the injection molding die is shown in FIG. When the blood is drawn, the syringe plunger in the syringe cylinder is up to the ring - (2) in Figure 1 - drawn. The stamp consists of a sealing ring (1), a handle (2) and 4 guide ribs (3).

These ribs contain 4 notches (4) to let the punch over the upper edge of the syringe-ylinder can be tilted. This will make the taking out of the stamp from the cylinder.

A first exemplary embodiment of a valve insert is shown in FIG. This consists of 2 parts, a sealing part (1) and a tilt valve (2) similar the part (1) in Figure 2. The lower pole of the rocker valve facing the blood cake contains several fine openings (3) which open into a wider channel (4). There are 2 indentations on the upper edge of the sealing part. The central one Indentation (5) is used to insert a rod made of plastic with which the valve insert is pushed towards the blood cake. Is the valve insert at the boundary layer Arrived between the blood cake and the serum, the rod is inserted into the indentation of the toggle valve (G) implemented. Further pressure down in the direction of the blood cake closes the tilt valve.

There is now no longer any connection between the bloodcake and what is above it Serum.

A second embodiment of a valve insert is shown in FIG.

Here ird a grid-shaped opening (7) in the sealing part t1), the the two phases (serum and blood cake after centrifugation) combine with one Slide (2) closed. This slide is in a guide disc (5) with the help of a rod made of plastic, which, as in the first embodiment example, at (4) is used1 postponed. A sealing ring (6) seals the sealing part against the syringe cylinder (corresponds to the sealing ring (7) in Figure 4).

A third exemplary embodiment of a valve insert is shown in FIG. This also consists of 2 parts: a sealing part (1) and a plate valve (2). The sealing part contains a central cylindrical opening (3). The plate valve is bent in cross section downwards in the direction of the arrow (8). Its outer edge contains Grooves for the passage of serum. The plate valve is so far in the sealing part introduced that the central opening (3) is closed and the outer edges on the annular shoulder (4) of the sealing part is seated.

The part that is put together in this way is then put into an injection molding cylinder with the help of eires plastic rod (5) inserted. The plate valve (2) is moved below (in the direction of the arrow) pressed, the central opening (3) opens. The after Arch shape arching at the bottom prevents the sealing part from extending beyond the wall of the syringe barrel is pressed. is the valve insert at the boundary layer between When the serum and blood cake arrive, the stick is removed: the plate valve due to its elasticity (e.g. polypropylene) lies down again on the central Opening (3) and closes it.

There is a sealing ring on the outside of the sealing part (6).

A fourth embodiment of the valve insert is also shown in FIG 6 shown. It is a pot seal (7). The cuff is minor designed conically. The cylindrical part of the cuff is towards the edge (9) thinly drawn out. The edge lies elastically against the syringe barrel. Will the Using a rod (5), insert the cup collar down into the syringe barrel in When pressed in the direction of the arrow (8), the serum will pass between the inner wall of the syringe barrel and outside of the cuff edge (9). If the pressure decreases, the edge of the cuff settles (9) back onto the inner wall of the syringe barrel.

An exemplary embodiment of a sealing plunger is shown in FIG It consists of a sealing ring (1), 4 guide ribs (2) and a central tube (3), through which the serum is pressed in the direction of the arrow.

The serum emerges from this central tube (3) at the upper end 2 side openings (4).

The entire system is operated in the following order: A needle is placed on the extension piece (5 in Fig. 1 and 1 in Fig. 2). To Puncture of a blood vessel is carried out using the punch (Fig. 3) in the syringe barrel Help of the attached stopper (9 in Fig. 1) and in the 2nd embodiment (Fig. 2) closed by turning the valve in the direction of the arrow. After that, the Stamp (Fig. 3) removed by tilting over the upper end of the syringe cylinder and the syringe barrel with a loose stopper (8 in Fig. 1 that on a pin is placed on the handle of the syringe plunger: 5 in Fig. 3) closed. now the device can be transported.

Either the excess serum is pipetted off or a valve unit with the help of a stick (to the course material for one-time use) between Ser @@@ and Rl @@ t @@ chen inserted, then the tilting valve of the valve insert is closed, by placing the rod in an indentation on the outside of the tilt valve (6 in Fig 4) in the direction of the blood cake, i.e. H.

is pushed down (or a slider over which the two phasers connector inserted, 2nd embodiment of the valve insert Fig. 5. A third and fourth exemplary embodiment is shown in FIG. 6).

There is now no longer any connection between the serum and the blood cake. The serum can be poured off without any special precautions. There could be several Stand for days over the blood cake, as there is an exchange of substances between the two phases more is possible.

The use of a stopper (Firg. 7) combines the lock of the vessel with a pipetting option. If you set the sealing stamp he the Serum in syringe cylinder, the syringe cylinder is on the one hand closed and on the other hand, the serum can be filled into daughter vessels at any time. To this For this purpose, the syringe cylinder is rotated by 1300 and placed in the daughter vessel and pressed in its direction. Serum enters through the sealing plunger the daughter vessel over.

Advantages of the described system: 1. The use of the syringe as a centrifuge tube avoids the usual transfer of the sample (and thus the risk of confusion and hemolysis).

Compared to the monovette and vacutainer principle, this has a description System, the advantage that the decrease according to the otherwise usual and proven syringe principle he follows. So there is no new, more complicated technique to learn. and vacutainer principle).

2. The system described causes the patient to be abgenomriene Sample can be collected, transported, distributed and stored in the same vessel. This eliminates the permanent risk of mixing up samples. The original label is retained and does not have to be duplicated.

3. The vertil insert - prevents re-mixing of the through centrifugation separate phases. Daker is a conversion of the serum into a another vessel is no longer required. In addition, the valve Technicon), no that fibrin threads are kept tight.

4. The sealing die combines the function of a sealing plug and a pipette to remove the serum.

After the 1st distribution immediately after inserting the valve insert the sample is usually stored in the refrigerator for subsequent analyzes and for several days Repeat examinations retained. To do this, the C vessel must normally be closed (risk of evaporation!). The sealing stamp fulfills this purpose. The system can therefore be placed directly in the refrigerator (with the sealing stamp facing up) will.

If something is required at a later point in time, this can be done as above some material can be removed at any time. That described one With these subsequent serums, the system avoids the otherwise necessary Removing the stopper, using a pipette and pushing the stopper back down. When the whole serum supply is used up, the sealing plunger is on the valve insert arrived.

Claims (5)

Patent claims: 1. Complete system for blood collection, centrifugation, subsequent Separation of blood cake and serum, and serum storage, thus g e n n z e i c h e t that syringe barrel with lockable needle attachment piece, valve insert and sealing stamps can be used. 2. Disposable syringes according to claim 1, g e k e n n n z e i n e t d ad u r c h that this was placed in a centrifuge and sealed at the lower pole can be, either by a plug with olive-shaped expansion or with an insertable tilt valve. 3. Syringe plunger according to claim 1, d a d u r c h g e k e ii n z e i c hn e t that there is a pin on the handle with a stopper to close the Syringe cylinder can be placed. 4. Closure valve according to claim 1, g e k e n n z e i c h n e t d a -d u r c h that with the help of a toggle valve or a slide and a sealing washer or a plate valve or a cup cuff, the two phases of blood cake and serum are completely separated from each other, so that an exchange of substances between not in both phases. more is possible. 5. Closure plug according to claim 1, d a d u r c h g e k e n n z e i c h -n e t that a central tube connects the two poles with one another. At one pole has a sealing ring with a central opening. The other pole is locked. The serum emerges from this closed opening at a side opening Pole off. Blank page