DE2156676A1 - Alpha tocopherol ubiquinone composns - biological stimulant - Google Patents

Abstract

Promoting the growth of plants, animals and microorganisms by applying a mixture of alpha-tocopherol and ubiquinone in a molar ratio of 1:19 to 7:3. The mixture may also contain a surfactant and an activator such as iron citrate.

Description

Biological activator and process for its manufacture I) The invention relates to a biological activating agent and in particular on a biological activating agent, the o6 -tocopherol and ubiquinone as effective Contains components. The means of the invention is capable of the life of individual creatures and tissues from animals, plants and microorganisms to obtain the biological To promote the activity of the same and the activity of fresh animal or plant products to be retained. It is ultimately able to control the growth of plants and to facilitate or ensure the root formation of trees or flowers.

Very often in daily life one encounters the need, the activity accelerate from living matter. This is not just in agriculture, Poultry farming, fishing industry and the like are the case, but also for various Fermentation industries to produce usable distant ent ation products that build on the reproduction of microorganisms. This requirement also applies on the transport of various fresh products that require the roservation of Individual organisms or organs and tissues of plants and animals, although the Propagation of individual evidence is not directly intended, too.

It is also the fundamental goal of medical science To avoid endangering the life that the activity is encouraged when the Activity of the human body due to injury or illness related to Has been partially or completely reduced.

In general it is known that all living things are made of water and proteins as basic components are composed and that their life and activities build on their physiological functions on the one hand and that on the other on the other hand, one's own life through the resistance to an oxidizing one or reducing condition is maintained0 when part of the tissue is through a wound or the like is penultimate and in direct contact with the oxygen comes into the air or when the reproduction of microorganisms takes place or when individual organisms from their appropriate evasion into a completely different one If transferred, the tissues or their cells can return to their normal state can no longer be maintained and lose their resilience.

As a result, Karl is no longer part or all of the right life be maintained.

Such phenomena occur when plants cut on one Incision to be planted, transplanted and the like or if due to pathogenic insects or climatic environments cause abnormal growth. Such phenomena are very strongly observed in plants and fruits. Even In humans and animals there are various diseases which are abnormal Appearances are due. Various phenomena also belong to these phenomena Everyday problems, such as the preservation and transport of fresh food Meat products, for example fish and the like It has now been found that the activities can be increased very effectively if one has a solution of <- -Totopherol and Ubiquinon heratellt and the solution to living things in a suitable concentration range. The appropriate ratio of α-Tocopherol and ubiquinone is in the range from t: 19 to 7 3.3, preferably 1: 4 to 3: 2. The correct concentration ranges of the components are in each case 10-15 Hol / l to 10-2 mol / l, preferably 10-14 mol / 1 to 10-6 mol / l.

So @ it was found to be an important condition that α-tocopherol and ubiquinone and others in an equilibrium in a given Molar ratio in a cell or in organs and the like, regardless of whether it is animals or plants are present so that they can carry out their normal life activities can continue. If, therefore, the living being is due to certain difficulties are brought into an abnormal state, then the abnormal living beings can again be returned to the original active state by touching the living beings. immersed in a solution of α-tocopherol and ubiquinone, which prepares reliably so that they take the desired concentrations @ within living beings and the living beings have no difficulty in their normal fiefdom activities LEVERAGING fertführen @.

When fresh animal or plant products with a solution of @ -Tocopherol and treated ubiquinone at the right concentration for the right time Then, on the basis of these principles, the fresh products can be converted into active products State and they can be in one state for long periods of time preserved in which there is no loss of freshness. In this Trap, the proteins and the other components are not destroyed and it finds no degradation in quality ». Furthermore, microorganisms can never reproduce1 so that no sterilization agents or sterilization treatments are required are. In this way, almost all problems of conservation and transportation can be avoided be solved by fresh products.

The present invention is also applicable to the growth control of Plants applicable. In general the higher plants have such properties that they always grow in dependence on the control of environmental factors. To the at the same time they accumulate certain components within their bodies accordingly the environmental factors at a certain stage of growth. The excess growth takes place, whereby the environmental conditions are met.

The relationships between the etoml) onents of plants and the plant growth, based on the environmental factors, investigated. being mainly the biological activity of the plants was taken into account. Thereby ends, that various phenomena of plant growth, including glow, ultimately can be determined by the ratio of o6 -tocopherol to ubiquinone. Furthermore, was found that when α-tocopherol and ubiquinone in the plant bodies are present in a certain molar ratio and with a defined concentration, the plants always in terms of growth regardless of the specific environmental conditions be accelerated.

Heretofore, it was generally believed that growth and temperature sensitivity of the plants is a property that is specific to the respective plant species is.

It was also believed to be an induction of flowering cannot take place under conditions unsuitable for flowering, namely notwithstanding the fact that Qb elie-environmental conditions are artificially changed, it is a so-called electrically illuminated chrysanthemum known, in which the blooming the chrysanthemum is controlled by electric lighting at night. However, the artificial change in environmental conditions requires large facilities and is very expensive. Therefore, with the exception of very special cases, it is almost impossible.

According to the invention, the environmental factors, which can cause great difficulties Brought for plant growth can be compensated by giving the plants externally administered a preparation containing α-tocopherol and ubiquinone, so that the molar ratio of α-tocopherol to ubiquinone to a certain Value is brought inside the plant body without affecting the environmental conditions to be changed. As a result, therefore, the restrictions imposed by the environment to be overcome.

According to the invention, the growth promotion can be in any Environmental conditions only by administering a solution of Q-tocopherol and ubiquinone to the seedlings or the roots of any plants via oblique short periods of time as shown in the examples. Even on condition those that do not cause flowering or fertilization of the plants can be the treated ones Plants have been brought to flower and fertilize. The restrictions on the plants through the environment not only regulate flowering, but also all Stages of growth, for example, nucleation, root formation, propagation of the plants and the same. These different growth phenomena can be rolled over by application of the present invention and the usability of the plants can thus be increased extremely.

Furthermore, the reproduction (i.e. the formation of roots) of plants done in a simple and safe manner according to the invention.

Plants are generally reproduced by onset a growing point of a plant that is difficult to grow from a seedling, into the ground or the like, whereby roots are formed directly from it, the sic propagate vegetatively. But there the readers or the cut branches or the growth points used for reproduction from the growing plant are cut off, the cut off parts are biologically an abrupt environmental change exposed. The activity of the cut branch is therefore considerably reduced and in the ordinary case, the water absorption is cut off after planting the Partly almost canceled. It is therefore used in plants where there is a loss of water through Evaporation occurs or where a long period of time is necessary for root formation is very difficult to maintain the activity of the plant until the roots take off have trained. So far, various attempts have been made by, for example the appropriate times selected for cutting off the movement of the water was prevented by the pruning and the like. These attempts are but not based on a substantial activation of the cut plants and it is therefore a matter of very specific measures that cannot be reproduced are. Even if such attempts are successful, they will be minor Activation is achieved and, in some cases, reproduction is different depending on the species of plants quite impossible.

@ e @ äß the invention has now been found that the Ilol ratio from α-tocopherol to ubiquinone must be in a certain range and that the α-tocopherol and the ubiquinone inside do @ plant body in one defined concentration must exist, so that the plant in any Environment can maintain its normal activity.

According to the invention, the activity of a cut branch it or a growth point to the original state ire: dom cutting off be traced back by the cut branch or the growth point that to be used for planting, immersed in a previously prepared solution @ingota in which the α-tocopherol and the ubiquinone in a certain molar ratio are present and the treatment concentration and the treatment time are set in this way be that the ob «/ -tocopherol and the ubiquinone inheren of the plant body available with the desired concentration. As a result, the absorption and the movement of water and nutrients through the cut off branch normally and the activity of the plant can be maintained without difficulty until the roots have developed from the cut branch.

It is naturally to be expected that when using a fertilizer or an agrochemical agent together with the discovery the time until the roots develop of the cut - @@ n branch can be shortened and the yield of the root - @@ l @ ung o @@@ ö @ t can be. Semit can have a higher value even with fields of a small area Effectiveness can be expected.

To produce a mixture of α-tocophorol and ubiquinone, that as an @iological activation agent for individual organisms or tissues of living Things are most effective, it is the least important, the mixture in a homogeneous aqueous solution. That is, the water-insoluble α-Tolco-Pherol and @@ ichinon are in the presence of a sedative in a homogeneous one Aqueous solution incorporated and used as an activity accelerator for the art of painting or used as an activity retaining agent. The ones used according to the invention Wetting agents are non-ionic wetting agents that are not effective against living bodies are toxic. Wetting agents of the polyoxyalkylene system are also suitable. In particular are polyoxyethylene sorbitol monostearate (Tween 60 trademark of Atlas Powder Co., USA) and polyoxyethylene sorbitol monooleate (Tween 80,) are most effective.

The preparation of the homogeneous aqueous solution of @ -Tocopherol and Ubiquinone happens in the following way: α-tocopherol and ubiquinone are weighed exactly and in a suitable order with a non-ionic wetting agent thoroughly mixed. Then the resulting mixture is slowly diluted with water, whereby the α-tocopherol and the ubiquinone are homogeneously dispersed in water. The resulting homogeneous solution is further mixed with water to the appropriate concentration diluted and used as a treatment solution. In this case it has been confirmed that a quantitative change in the wetting agent is not a direct reference to the Has activating effect of the treatment solution on living bodies. To stop one If the aqueous solution was homogeneous, it is sufficient to add the oC -tocopherol and the ubiquinone with a wetting agent in 10 to 20 times the total weight of ven α-tocopherol and ubiquinone to mix. Usually the α-tocophenol and the ubiquinone in the aqueous solution in each case with a concentration of 10-3 to It contains 10-4 mol-1 and contains the wetting agent at a concentration of 0.2 Up to 0.5% by weight based on the total weight of the aqueous solution. After that the L5 solution is diluted to the desired concentration.

The aqueous solution of α-tocopherol produced in this way and ubiquinone having a suitable concentration has a affinity compared to living bodies. The dispersibility of the processing solution the living body is very excellent.

However, the treatment solution prepared in this way is opposite Air (oxygen) and light are relatively sensitive, which affects their chemical properties from α-tocopherol and ubiquinone. There is therefore the danger that their strength slowly diminishes. Even if the treatment solution is in one light-shielding vessel or in a preservation vessel where air is passed through an inert gas has been replaced, is kept, the deterioration is often quite a bit unavoidable.

According to the invention, the power of the treatment solution from α-tocopherol can now and libichinon saved from deterioration and the treatment solution over extended periods of time can be made stable by following the treatment solution treated. The homogeneous aqueous solution of α-tocopherol and ubiquinone can be stabilized by bringing the solution to a temperature of 70 ° C up to the decomposition temperature of the α-tocopherol under atmospheric pressure or under pressure for a short time Period, e.g. ß. 5 minutes to 30 minutes immediately after making the solution is heated. The solution can be extended in this way in a stable state Periods of storage if kept in a light-shielded condition will.

In addition to the procedure described for the production of a Treatment solution using wetting agents can be a clear homogeneous aqueous Solution of α-tocopherel and ubiquinone can be prepared by adding α-tocopherol and ubiquinone using hydrophilic, nucleophilic, polar organic Solvents as a dispersing medium is made water-soluble. According to the Solvents used in the invention are such nucleophilic solvents as Dimethyl sulfoxide, dimethylformamide, dimethylacetamide, tetrahydrofuran and tetramethylphosphoramide. There these solvents have a high dissolving activity for the water-insoluble α-tocopherol and ubiquinone also have a high affinity for water, this can Solvent the α-tocopherol even when using relatively small amounts and make the ubiquinone water soluble. The use of a solvent in a An amount of 10% by weight or less based on the resulting solution is sufficient. It is well known that dimethyl sulfoxide, dimethylformamide, etc., because of their properties Polarity or high affinity have high permeability and that they are widely used have excellent permeability even when applied to living Body to be awakened. In particular, the dimethyl sulfoxide is used as a drug valuable. These solvents are therefore very beneficial for the homogeneous and rapid Dispersion of α-tocopherol and ubiquinone in the individual organisms, if these be treated with the treatment solution.

As already stated, the aqueous solution is α-tocopherol and ubiquinone relatively unstable and it is caused by exposure to light or dissolved oxygen rapidly decomposes. These solvents have, however, as nucleophiles Solvents have excellent dissolving properties and they work beyond that lii considerable scope as a radical scavenger. these solvents therefore also work as stabilizers.

As already stated, there are different types and ways available, ur! the activity of various living bodies and Obtain tissues using ¼ α-tocopheral and ubiquinone. It it was found that there are substances that are capable of the α-tocopherol-ubiquinone system @ in the living bodies in a stable state. It was found, that one of these substances with such an effect is iron ions. In this Iron citrate, iron chloride, iron sulfate and the like can be used as egg compounds be used. Since the IMsenionen are involved in the stabilization of the α-tocopherel ubiquinone system @ Participate, the addition of the ice compound as an activator can be more positive Have a wider effect on the abnormality of living bodies, which can be attributed to various reasons. The iron ions are in abundance used which are equivalent to or above the α-tocopherol.

The aqueous solution of α-tocopherol and ubiquinone, the produced according to the described working methods with the appropriate concentration become i54 ', possesses an affinity for living bodies, you possess also very good dispersibility into living bodies. Thus can the dispersion of α-tocopherol and ubiquinone in living bodies or their tissues through the known working methods such as absorption, immersion, Surface coating, spraying and the like can be done very well. Furthermore can activating the living body and maintaining freshness through this treatment can be targeted.

The invention is illustrated in the examples.

Example 1 4.3 mg α-tocopherol and 100 mg irritant (Tween 80) were mixed with 100 ml of distilled water, whereby a solution A was obtained which had a concentration of 10-4 mol / 1.

6.0 mg of ubiquinone and 100 mg of the above heretic agent made 100 ml of distilled Water mixed, whereby a solution 3 was obtained, the concentration of which was 10 t Mol / 1 was. -By preparing samples with appropriate volumes of solutions A. and B and mixing these samples and diluting the mixture obtained into the desired volumes were obtained seven types of solutions, in which the proportions α-tocopherol and ubiquinone 1: 0.8: 2.6: 4.5: 5.4: 6.2: 8 and 0: 1 were. The total sum of the diols of α-tocopherol and ubiquinone was but kept at 2 x 10-7 MOl / 1.

Radish seedlings (type of comet) that had been sprouted in advance became immersed in each of the above solutions for 30 minutes. Thereupon were The seedlings are immediately planted in 1/5000 A pots, which are filled with quartz sand was. In a brood chamber with continuous lighting (30,000 lux) ge during the day and at night at 25 ° C. Each pot contained five radishes "The five Radishes, however, were reduced to three per pot after a week. The seedlings were grown in the control experiment, using distilled water instead of the solutions was used.

In Table I is the stem length and flowering of the radishes after Specified 45 days after the start of cultivation.

Table I α-tocopherol / stem length blooming ubiquinone control experiment 2.7 cm undifferentiated 0/1 12 cm buds 2/8 13.5 cm buds 4/6 13 cm buds 5/5 21 cm flowering G / 4 7 cm buds 8/2 2.3 cm not differentiated 1/0 0.5 cm not differentiated Example 2 α-tocopherol 4.3 mg ubiquinone (coenzyme Q7) 6.6 mg Retzmilel (tween 80) 200 mg distilled water 100 ml By By mixing the above ingredients, emulsimony obtained became 100 times the original Volume diluted with water. The resulting solution became seedlings of bindweed (Type: large-flowered purple) given. These were previously 30 minutes on a Petri dish germinated. The seedlings were then immediately placed in 1/5000-a pots, which were filled with quartz sand and transferred to an incubation chamber at 25 ° C. night and day grown with continuous exposure (luminescent lamp: 4000 lux). Everyone Pot had five seedlings. Seedlings were also grown in the control experiment, using distilled water in place of the solution, as a result to establish that all plants} their seedlings with the solution of the £ <t α-tocopherols and ubiquinone had been treated as to of the flower buds had differentiated. The control experiment took place in one Cultivation for five months after sowing, no cultivation at all. Table II shows the change in the proportion of flowers over time.

Table II Differentiation for the flower buds and the flower ratio Treatment; 50 days after sowing 85 days after sowing Differen- Flowered Differ- Flowered adorns for adorns for flower buds- flower buds pen solution of d-tocopherol and ubiquinone 100% 0% 100% none (control experiment) 0 0 0 0 example 3 The emulsion of Example 2 rmrcle with water to 1000 times the original Volume diluted. The resulting solution became the seedlings of soybeans (Species: okuhara beans) which had been allowed to germinate 30 minutes in advance. The seedlings were sown in pots which had been filled with soil. In a Glass chamber was cultivated at 30 ° C. The tests continued for 23 days. Dic The results obtained are shown in Table III.

Table III Treatment Length of the number of fresh-weight root nodules Grass leaves of the plants each 5 plants above- below- Number fresh ground the adgo weight and hesions solution of α-tocopherol and ubiquinone 71a2 cm 7.0 3.6 g 1.0 g 53 442.9 mg none (control experiment) 49.8 cm 6, o 2.7 g 0.6 g 22 112, (mg Example 4 The emulsion of Example 2 was 100-fold with water original volume diluted thereby creating an aqueous solution in one concentration of 10 was obtained in a concentration of 10 mol / l (solution A).

Furthermore, an aqueous solution was prepared containing 1000 ppm of ice citrate, expressed as Fe (solution B). 10 ml of solution A and 10 ml of the solution 6 were added to 980 ml of distilled water and mixed thoroughly with stirring, whereby a solution C was obtained.

Cucumber seedlings were trained in a farm. 50 c @ m of the solution the leaves were taken out once on their two days ling sprayed. The servants were cultivated in the usual manner for 67 days. At the same At that time, seedlings were grown and the solution C was sprayed with tap water became.

In Table IV are the total yields during this cultivation period shown.

Table IV Treatment Total Yields / Number of Seedlings Number Weight (g) A solution of α-tocopherol and ubiquinone (coenzyme Q7) 9.0 1172 Heine (Control experiment) 4.6 53 Example 5 The emulsion of Example 2 was 30 minutes heated at 120 C in an autoclave. The resulting solution was made with water diluted to an aqueous solution which has a concentration of 10 7 I-lol / l jat. 10 eggplant seedlings were placed in pots filled with earth. The relationship was one seedling per pot. After two days, 200 ml of the solution with the concentration of 10-7 mol / 1 is injected at the roots of the eggplant seedlings. The seedlings were grown in a greenhouse at 25 ° C to 30 ° C for 58 days, adding to the Seedling in suitable amounts of ammonium sulphate and nitrogen and potassium phosphate fertilizers were given. At the same time, seedlings were grown as a control experiment, which had been sprinkled with water instead of the solution. The results obtained are compiled in Table V.

Table V Treatment total yield / seedling number average Weight (g) A solution of α-tocopherol and ubiquinone (coenzyme Q7) 2.4 132 None (control) - 92 Example 6 The emulsion of Example 2 was made with Water diluted to 100G times the original volume. The cut ends of 20 cut rose branches (species: Early Francis) with a length of 20 cm with 4 connecting sheets were immersed in the resulting solution and off taken from the solution after 30 minutes. Then 5 cuttings per pot were in 1.5 1 planted flower pots, which were filled with quartz sand, and rinsed them thoroughly with water filled. the pots were in a glass chamber at 23 ° C during the day and 18 ° C at night given. These plants, their cut ends in distilled water in place of the solution were dipped in the same manner as control plants drawn.

The percentage survival of the cuttings and the cultivation process are given in Table VI. The cultivation was carried out for 74 days.

Table VI Treatment Number of Percentage Cultivation Processes Survival A solution from: O'-Toco- and the leaves were from the pherol and 20 100% start of planting and ubiquinone pruning not changed, auxiliary buds were pulled after a month and the new branches were grown quickly after two months extended.

None (Kon- 0 0% The activity of the plant roll test) zein was after reduced after planting. The leaves gradually weathered and peeled off. the Branches turned brown after a month and water absorption stopped.

The auxiliary buds were all weathered. The activity of the stalk Example 7 The emulsion of Example 2 was made 1000 times original with water Volume diluted. Cut branches of maple with four connecting leaves and a length of 25 cm were immersed in the resulting solution, after 30 minutes The twigs were removed from the solution and three twigs were made, in three rows planted in a 1/5000-A pot filled with soil. After planting and cutting were the pots near the window of an atmospheric temperature chamber ture arranged from 20 to 230C. Thoroughly water was added. The pots were ditched.

Those cut cuttings that are only used in the distilled water were also planted as a control. It was 58 days Tests carried out. the Formation of the cut branches and the isolation process are summarized in Table VII.

Table VII Treatment Number of Percentage Cultivation Process A solution The activation of the stems of α-Toco- and the leaves were not changed after the pherol and planting ubiquinone 9 100%. The green color of the leaves was not lost.

The water absorption by the cut ends was continuous Further. The leaves showed a natural red color after 1 1/2 months. The auxiliary buds were thick and there was callus formation at the cut end and besides roots had formed None (control experiment) 0 0% After 5 days of planting the leaves began to weather. Gradually they had furried and fell away. After a month all parts were weathered.

Example 8 α-Tocopherol 4.3 mg Ubiquinone (Coenzyme Q7) 6.6 mg Dimethyl sulfoxide 100 ml The components mentioned were mixed with one another. The resulting solution was diluted with water to form a solution that contained α -Tocopherol and ubiquinone each with one concentration from 10-7 Mol / 1 contained (solution A). Furthermore, a solution @ was prepared that this contained both substances with a concentration of 8 x 10-13 mol / 1 (solution B). Then one end of 20 sprouts (18 cm) of spindle trees was pruned End immersed and left in solution A for 30 minutes at normal temperature. Likewise, 20 offspring thereof were treated in a similar manner iii and in the Leave solution 13 for 24 hours. Then they were taken out of the solutions. The cut ends were washed with water.

5 heads of them were planted in a pot, the one with black earth was filled. The cultivation took place in a greenhouse at temperatures of 25 to 28 ° C per day for a period of G7 days. Table VIII shows the obtained Results. As a control experiment, it was also immersed in water.

Table VIII Treatment Percentage Root Wax Growth cn / Root Survival tum g / root-drawn cuttings cuttings Solution of α-tocopherol and ubiquinone (10-7 mol / 1) 90 0.171 3.98 solution of α-tocopherol. and ubiquinone (8x10-13Mol / 1) 100 0.479 12.19 None (control experiment) 80 0.114 2.02 Example 9 α-tocopherol 4.3 mg ubiquinone (coenzyme Q7) 6.6 mg Wetting agents (Tween 80) 200 mg distilled water 100 illl The components were mixed together mixed and the resulting emulsion was diluted with water to form a solution, the »-Tocopherol and Ubiquinon each with a concentration of 10-5 mol / 1 (solution A), and to a solution that boiden each with a concentration of 8 x 10-11 mol / 1 (solution B). In the same way an aqueous solution was prepared which contained 1000 ppm iron citrate, expressed as Fe (solution C).

10 ml of solution A and 10 ml of solution C were distilled to 930 ml Added water and mixed thoroughly with stirring to obtain a solution D. became. Similarly, 10 ml of solution B and 10 ml of solution C became 980 ml added distilled water and mixed thoroughly with stirring, creating a Solution E was prepared, then one end of 20 '(13 cm) sprouts was removed from Spindle trees immersed in solution D for 30 minutes and another 20 sprouts were treated in the solution E for 24 hours, then the sprouts were in pots planted in the same manner as in Example 8. The spindle trees were in in a greenhouse for 67 days at 25 to 230 C I; The results are in the table IK shown. As a control experiment, it was also immersed in water.

Table IX Treatment Percentage of Root Growth g / Growth cm / Root Overlap Root-pulled, cut cuttings are a solution of α-tocopherol and ubiquinone (10-7 mol / l) 80 0.206 6.35 Table IX continued Treatment Percentage of root growth g / growth cm / root survival Root-pulled Drawn cut cuttings are a solution of α-tocopherol and ubiquinone (8x10-13Mol / 1) 100 0.289 9.98 None (control experiment) 80 0.114 Example 10 The emulsion of Example 9 was heated to 130 ° C. in an autoclave for 30 minutes. The resulting Emulsion was made with water u a solution containing 06-tocopherol and ubiquinone respectively with a concentration of 10-7 Nol / 1 (solution A), and a solution containing the two Contained substances with a concentration of 3 = c 10-13 Hol / l (solution B), diluted. On one end of 20 sprouts (18 cm) of spindle trees on cut end dipped and at normal temperature In solution A 30 Leave for minutes. Another row of 20 sprouts was made in the same way Treated with solution B for 2½ hours. Then they were taken out of the solutions. The cut ends were washed with water.

5 sprouts were each planted in a pot, the one with black earth was filled. The cultivation took place in a greenhouse at 25 to 28 ° C. during the day over a period of 67 days. The results are shown in Table X below. A control smoke was carried out using water in the usual manner.

Table X Treatment Percentage @ Root Growth S / Growth cm / Root Survival Root-pulled, cut cuttings are a solution of α-tocopherol and ubiquinone (10-7 mol / 1). 90 0.284 4.57 Solution of α-tocopherol and ubiquinone (S x 10-13Mol / 1) 100 0.342 8.74 None (control experiment) 80 0.114 2.02 Example 11 The emulsion of Example 9 was made up to 100 times the original volume with water diluted. The cut ends of 10 cut branches of pine (black @ pine) with green leaves that grew in the mountains (length 25 cm including the leaves), were immersed in the resulting solution and taken out after 30 minutes. Then each 5 cut branches were in a 1.5-1 flower pot, which with Quartz sand was filled, planted. @ It has been thoroughly filled with water. the Cultivation took place in a glass chamber at 23 ° C during the day and 18 ° C at night over a period of 86 days. The corresponding values are given in table @I ang @.

Table XI Treatment Number of Percentage Cultivation Process Survival gene solution of α- No change in tocopherol and 10 100% green color of the U @ ichinon leaves after planting the cuttings. No trouble at the cut ends of the Zweigo. After three months of The planting was done at the cut ends as a precursor to root growth a callus formation is observed.

None (con- The plants were trolled) 0 0% month after green after planting, but then turned deep brown. the water absorption of the total official rehearsal was canceled. The leaves weathered.

Example 12 The emulsion of Example 9 was made in an autoclave Heated to 120 ° C for 30 minutes. The resulting solution was diluted with water Diluted 1000 times the original volume, producing a solution the α-tocopherol and ubiquinone in each -7 a concentration of 10 7 Mol / l contained.

The cut off ends of 25 sprouts (15 cm) of the Japanseder (made in Masuyama, Toyama Prefecture, Japan) was immersed in the solution for 30 minutes einet @ ucht. In each case 5 sprouts were planted in a pot. Overall were Scramble 5 pots.

Soil was filled into the pots. The cultivation took place in a greenhouse at 25 to 30 ° C per day for a period of 133 days. in table XII the results obtained are compiled. An experiment was made for comparison made with water.

Table XII Treatment Percentage Root Growth Solution of @@ - Tocopherol and ubiquinone 84% none (control) 52% Example 13 The emulsion of the example 9 was diluted with water to 100 times the original volume. Cut off Flowers of a rose (species: Early Francis, flower with 5 leaves) were in 30 minutes the resulting solution is immersed and taken out of the solution. The rose became then placed in an Erlenmeyer flask filled with distilled water was. The flask was placed in a chamber with good ventilation at an atmospheric temperature brought from 25 to 30 ° C for 16 days. A rose that was put in water instead of the solution was dipped was used as a control. The degree of weathering of the cut flowers and the turbidity of the water were observed. Tabel XIII shows the results. The numerical values in the table are average values of 10 attempts. Two roses were used in each experiment.

Table XIII Treatment Degree of weathering (%) Turbidity of the water 2nd day 3rd day 16th day 2nd day 3rd day 16th day Dissolution of α-tocopherol and ubiquinone O 0 0 - -None (control experiment) 25 100 100 + + + Example 14 The Emulsion of Example 9 was made up to 100 times the original volume with water diluted. A cut lily flower (stem length 50 cm, blooming) was in the resulting solution was immersed for 30 minutes and removed therefrom.

The lily was placed in an Erlenmeyer flask filled with ~ distilled Water was filled, the flask was placed in a chamber with good ventilation at atmospheric temperature given from 28 to 32 ° C for 12 days. Table XIV shows the results obtained. A control experiment was carried out with water.

Table XIV Treatment activity of flower, stem and Leaves 2nd day 4th day 11th day Solution of α- No change- No change- None Changes in the tocopherol and the freshness of the fri-ubiquinone freshness cal of the flower, cal of the flower, flowers of the stem of the stem stem and and the leaves and the leaves of the leaves None (Rontroll- The Freshness The Bloom was' the fabric of the experiment) was weathered and bloom and the velvet the color of the leaves was broken leaves was broken destroyed, rt Example 15 Die The emulsion of Example 9 was made up to 100 times its original volume with water diluted. The cut flowers of blooming carnations were in the 30 minutes resulting solution dipped and taken out from it. The clove was placed in an Erlenmeyer flask given, which was filled with distilled water.

The flask was placed in a chamber that had good ventilation had and in the atmospheric temperature from 28 to 320C before lay. This was done for 12 days to observe the weathering grass. The current ones Results are shown in Table XV. A control experiment was carried out with water.

Table XV treatment activity of stem and leaves 3. 9th day 6th day 11th day Solution of α- no changes- no changes- no changes-tocopherol and the freezing of the freezing of the Fri-Ubiquinon cal none (control The bloom was the upper part of the blooms which tried) weathered of the stalk stalk and the leaves and the leaves were almost barred and scented white Example 16 The emulsion of Example 9 was increased 1000 times with water original volume was diluted to give a solution containing α -Tocopherol and ubiquinone each contained in a concentration of 10-7 7 mol / l. The resulting solution was further diluted with water to a solution that contained these both components in a concentration of 8 x 10-11 mol / 1 (solution A) contained. Separately, an aqueous solution was prepared containing 1000 ppm of iron citrate, expressed as Fe (Solution B).

10 ml of solution A and 10 ml of solution B were distilled to 930 ml Added water and mixed thoroughly while stirring.

The cut end of the cut flower becomes a non-flowering one Tulip (total length 35 cm) was added to the resulting solution and incorporated therein Leave room temperature for 24 hours.

The tulip was then placed in a flask containing a solution containing a source of nitrogen, phosphates, potassium salts and other nutrients. The flask was placed in a cultivation chamber at 15 ° C with continuous lighting day and @acht (mercury lamp: 30,000 lux) given. Likewise a control experiment with water was added. Table XVI shows the change the activity of the a @ cut flower tone before and after treatment.

Table XVI Treatment activity of the flowers, the stalk and the Leaves 3rd day 5th day 8th day Solution of Die Blätter and Die Blätter and Die Blätter and α-Tocophe - the flowers were the flower stalks - the flower stalks rol and all fresh and gel were long- were both gone-Ub @ chinon had their gel and a noticeable bloom that Diksamte had and had considerable activity grown out of length.

forms The entire parts had maintained their activity continuously, there was considerable bloom formation with a considerable color.

No (con- The tips of the freshness of both the flowers trollver- Leaves were leaves and the flower stems turned brown as well as the petals all samples and the ends were lost were weathered were received. It has been successful. The shriveled one looked weathered, the coarse the stripped color tone the cut ends of the ends had been broken up and the opacity of the nutrient fluid was disturbed was significant, Example 17 The emulsion of Example 9 was diluted with water, whereby a solution was obtained containing α-tocopherol and ubiquinone, respectively with a concentration of 8 x 10-11 mol / 1 (solution A). Furthermore, a Aqueous solution prepared containing 1000 ppm iron citrate, expressed as @e (Solution B).

10 @@ of solution @ and 10 ml of solution B were distilled to 980 ml Given water and mixed thoroughly while stirring, whereby a Solution C was made.

The root parts of 50 1 year old red pine seedlings were made immersed in the solution C at the normal temperature for 24 hours and in a Farm planted. Over a period of 106 days, the condition and that Growth of the seedlings observed. As a control experiment, water was used. The results are shown in Table XVII.

Table XVII Treatment Average Percent Growth (mm) Survival solution of α-tocopherol and ubiquinone 5.1 98 None (control experiment) 2.9 72 Example 18 The emulsion of Example 9 was increased 108 times with water original volume diluted to give 5 l of a solution o ' -Tocoplierol and ubiquinone with a final concentration of 10-12 mol / 1 each. In addition, it was diluted to 1010 times the original amount thereby obtaining 5 liters of a solution containing these two components respectively contained in a final concentration of 10-14 mol / l.

Separately from these were 18 flasks with a capacity of 500 ml prepared round bottom. In the flasks were cut flowers of roses with one bud per flask and immersed in water in an exposed chamber of Cultivated at 25 ° C, whereby the rose petals in the flasks (rose species: Early Francis Superstar) were let out. Two Days were the Let the roses bloom and split them into three groups. Each group was in the solution immersed, which contained the two @ o @ components in a concentration of 10-12 mol / 1. @s was sealed with a rubber stopper to prevent airborne contamination to prevent. At that time, the tips of the clipped pointers were covered with nettles cut to expose the surface and between the rubber plugs and hold the plunger. Other groups were included with the solution, which the two components each in a concentration of 10-14 mol / 1 in a similar way Way contained. A control experiment was carried out with water.

The roses prepared for this catfish were kept in a chamber atmospheric temperature of 22 ° C for 40 days with the flask turned upside down so that the flowers of the roses could stand upright. Were in this way obtained the following results.

a. a solution with ° C-tocopherol and ubiquinone jewoils in one concentration from 10-12 Nol / 1: The rose petals were different from the original facings not changed if they were tightly sealed in the flask. the ends that The color of the leaves and the stems were kept fresh. The activity of the, blossoms could be maintained 40 days after the treatment without reproduction of microorganisms appeared, i.e. the solution remained clear.

@. A solution with α-tocopherol and ubiquinone at one concentration of 10-14 mol / 1 each: the freshness of the rose petals went off after one day Lose treatment. The @estandtoile of the roses were given in the L @@ ung. @@ @and a considerable follow-up from t @@@ orgunisms instead of. The solution became cloudy. The @webs of the roses were from the treatment after three days broken up. A spoilage was found in the flask.

c. Control experiment: The freshness gradually went off after two days lost the treatment. At the same time the reproduction of the microorganisms increased gradually to. After six days from treatment, the tissues were broken and the corruption had progressed rapidly in the flask.

Example 19 The emulsion of Example 9 was applied to the Diluted 1000 times the original volume. 10 fruits of eggplant were made in parallel dipped in the resulting solution, leaving the cut ends in the solution immersed. They were removed from the solution after 30 minutes. On that the fruits were immersed in ordinary tap water in a parallel experiment. The trough was kept at 22 ° C with good ventilation.

As a result, it was found that when using water in the Control the tissues of the cut ends after two days of treatment had broken up and that the reproduction of the microorganisms around the cut off Ends horum had begun. The solution had turned cloudy. The original shine the fruit was lost. However, the freshness of the treated had increased Fruits did not change and the solution was not cloudy. The treated fruits had not differed from the original condition after a @onat after the treatment. On the other hand, the control experiment was spoiled. The treated samples were kept fresh.

@ example 20 The emulsion of @ example 9 was with water on the 108-fold original volu @@ en pre-thinned, whereby a solution was obtained which α-% ocopherol and ubiquinone each with a concentration of 10-12 mol / 1 contained at the end. 10 live crucian fish were placed in a 500 ml plastic kettle (erucians), which was filled with the resulting solution.

The vessel was covered. The active movement of the cross fish was stopped after 24 hours. Then they were in the same solution at room temperature left and closed thoroughly. With distilled water a Control experiment carried out.

As a result, the tissues of the cross fish were after a day of the reservation broken up in distilled water. The reproduction of Mihroorgani @ men was important. The components of the fish bodies had been given up. The entire Water was turned into a muddy state. On the other hand were the cross fish kept in the solution in the fresh state and the solution remained clear. There was no breakage of the tissues. Even after 13 days No reproduction of microorganisms was observed from the treatment and the freshness was retained.

Example 21 The emulsion of Example 9 was applied to the Diluted 1000 times the original volume, thereby obtaining a solution which o & -Tocopherol and ubiquinone each with a concentration of 10-7 mol / 1 contained. The resulting solution was further separated to 6 x 10-13, 6.5 x 10-13, 7 x 10-13, 7.5 x 10-13 and 8 x 10-13 mol / 1 diluted. These solutions were placed in 200 ml glass bottles. A tropical fish (nech tetra) given. Then the bottles were closed close to @@. With water was a control test carried out. The lifetimes of the fish according to the treatment are summarized in Table XVIII.

Table XVIII Control Treated Trial 6x10 @@ 6.5x10 @@ 7x10 @@@ 7.5x10 @@@ 8x10 @@@ Lifetime (days) 3 5 14 51 1 Example 22 The emulsion of the example 9 was diluted with artificial sea water to 1000 times the original volume, whereby a solution was obtained containing α-tocopherol and ubiquinone, respectively at a concentration of 10-7 mol / 1. In a plastic trough (36 x 30 Ä 5 cm) Sea sand was given to a depth of 3 cm. The resulting solution was placed to a depth of 4 cm. i4.0 insects (Marphysa sanguinea) were made put in the trough and kept in a refrigerator at 100C as treated "insects. The same was done with such insects that were found in artificial lake water instead of the solution. As a result, it was found that the untreated insects began to die after three days and that 80%, of the untreated insects were dead after 12 days. This can be seen from the table IXX emerged. On the other hand, 40% of the treated insects were still after 12 Days been killed. The tissues of the killed insects were not so broken as in the control experiment. The freshness was maintained so that the insects could be satisfactory could be used as bait for fish.

Table IXX Treatment After 3 days from After 12 days from the practitioner Treatment None (control 70% lived, 20% lived, with the tissue troll test) of the killed insects were severely broken solution that lived α - 100%, 60% lived. The tissue was tocopherol and remained elastic and almost at all The ubiquinone- no changes-killed insects were used as holding bait used for fish.

Example 23 The emulsion of Example 9 was applied to the Diluted 1000 times the original volume, thereby obtaining a solution which α-tocopherol and ubiquinone each with a concentration of 10-7 mol / 1 contained. The resulting solution was further diluted with water to 8 x 10-11 mol / l (Solution A) diluted. Separately, an aqueous solution was prepared containing 1000 ppm of iron citrate than Fe contained (solution B).

10 ml of solution A and 10 ml of solution B were distilled to 980 ml Added water and mixed thoroughly while stirring.

10 live clams were put in the resulting solution and Maintained at room temperature for 24 hours. Then 10 clams were divided into two groups divided with 5 mussels each and placed in plastic vessels (9.5 x 9.5 x 9.5 cm), which were filled with 50 ml of distilled water, given. The vessels were sealed and kept at room temperature continuously. Those clams made with distilled Water treated in place of the solution was used as a control and treated accordingly. In Table XX is the number of live clams according to the treatment along with the percent survival.

Table XX After two days After 4 days After 7 days Treated 10 (100%) 10 (100%) 10 (100%) Untreated (control experiment) 5 (50%) 2 (20%) 0 (0%) Example 24 The emulsion of Example 9 was made 1000 times original with water Volume diluted, producing a solution containing α-tocopherol and ubiquinone each at a concentration of 10-7 mol / l. The solution was further diluted to 10-12 mol / l with water.

20 corbiculas were added to the solution with a concentration of 10-7 / 1 Immersed for a day and removed from it, transferred to water and at room temperature Stored in a well sealed condition.

Another 20 corbiculas were added to the 10-12 mol / l solution and stored similarly. The ones treated in water instead of in the solution were kept in the same way. In Table XXI are the obtained Results compiled after 30 days.

Table XXI Percentage Survival of the Corbiculas After 12 After 16 After 21 After 23 After 30 Days Days Days Days Treated 10-7 mol / l 100 100 100 90 @ 0 10-12 Mol / l 100 95 95 85 0 Untreated (control experiment) 75 65 0 0 0 Example 25 The emulsion of Example 9 was made 1000 times original with water Diluted volume to make a solution, clie -tocopherol and ubiquinone each contained in a concentration of 10-7 mol / l. The solution became wider diluted with water to 8 x 10-13 mol / l.

meat from living aukp casings that are just being freed from the casings was placed in a 900 ml glass jar. It was at room temperature kept sealed. In the control experiment, it was immersed in distilled water.

The change in the flesh of the Ark casings after the treatment will be given in Table XXII.

Table XXII After 5 Days After 15 Days After 40 Days Treated None Cloudiness The shape of the The shape of the flesh in the solution was flesh was good good untreated considerable damage spoilage of the meat (control experiment) The liquefaction in the meat had been considerably liquefied. Example 26 The emulsion of Example 9 was made up to 1000 times the original volume with water thinned, a portion (1.5: 1.5 cm) of hair on all the sides of white rats (species: DDK, weight: 20 g) was cut off and the skin was artificially applied to this part damaged with 0.2 ml of 18N sulfuric acid. After 7 minutes from the Schwofolic acid treatment the solution in degreased cotton was applied to this part. In a comparison experiment the same procedure was used with distilled water.

The results obtained are summarized in Table XXIII, Five rats were used in each group. The rats were in one Incubator kept at a mean atmospheric temperature of 23 ° C for 24 days. Tabel XXIII State of Prevention Treatment After 2 hours After 10 hours After a Day After 2 After 5 After 10 Days Days Days Treated By Sulfur The skin was The skin was The skin The skin Not acid was not abnormal not abnormal. was not was normal no chewing was not treated with abnormal disability Part times. normal.

watch out for the growth of hair. The hair-hair growth grew of- almost to fensicht-zum urlich. original condition.

Control experiment The skin surface The skin surface The skin had it The fabric was rough and it was torn a red fabric Both of them were swollen and broken and red, mor and brooding know. tissue in rats was broken and it was broken. the one killed. substantial Rat suppurated ter 5 instead. It died. a white-gray scab appeared.

Example 27 The emulsion of Example 9 was applied to the Diluted 1000 times the original volume, thereby obtaining a solution which α-tocopherol and ubiquinone each in a concentration of 10-7 mol / l contained. this solution was further diluted with water to 3 = c 10-11 mol / l (solution A). Separately, an aqueous solution was prepared expressing 1000 ppm iron citrate as Fe (solution B).

5 ml of solution A and 5 ml of solution ß were commercialized in 490 ml Given milk and shaken thoroughly in a blender. The solution became dense bottled and kept at room temperature.

In the same way, a milk was produced in a comparative experiment, which had been mixed with distilled water. In Table XXIV is the state the milk indicated after the treatment.

Table XXIV After 4 days After 7 days After 14 days P does not act spoiled not spoiled- not spoiled ben untreated soured perfectly ver different Bal (control test) and partly their and almost teries grew congealed completely in the withered bone and fermented parts.

The mass became viscous,

Claims (1)

P a t e n t a n s p r ü c h e 1. Biological activating agent, which can maintain and promote the biological activity and that the freshness can be retained, in that it is indicated that it is α-tocopherol and contained ubiquinone in amounts of 1:19 to 7: 3 as active ingredients, 2nd method for the production of a biological Alitivie- @ ungs @ ittels according to claim 1, characterized , e k e n n z e i c ii -n e t that one α-tocopherol and ubiquinone as active ingredients mixed with a hydrophilic, nucleophilic, organic solvent, whereby the Active ingredients are made water-soluble or emulsified and with the amount of the hydrophilic, nucleophilic, organic solvent 10% by weight or less, based on the resulting mixture, is. 3. Process for the preparation of a biological activating agent according to claim 1 d a d u r c h g e k e n n n z e c hn e t that one α-tocopherol ver @ ischt with ubiquinone as active ingredients and that the resulting mixture is on a temperture of more than 70 ° C and lower than the decomposition temperature of the α-Tocopherol heated at atmospheric pressure or under Druc @. 4. Process for the production of a biological activating agent2 according to claim 1, d a d u r c h e k e n n n e i c hn e t that @an α-Tocophorel and ubiquinone as active ingredients with 0.2 to 0.5% by weight of a ni @ hionegenic inflammatory agent verll @@@ @@@ eh @ than 70 ° C and a lower temperature than the decomposition temperature α-Tocopherol @ heated under atmospheric pressure or @@ uck. 5, Process for the preparation of a biological activating agent according to claim 1, characterized in that there are no. that one α-tocopherol and ubiquinone as active ingredients mixed with an iron compound as a stabilizer, the amount of the iron compound being equivalent to that of the α-tocopherol is or is greater.