DE2153387B2 - Method for the photometric determination of chloride ions in body fluids - Google Patents

Claims (3)

3 2 constants allowed. In addition, the heavy metal ion must produce a detectable color with the complexing agent. 1. Method for photometric determination The subject of the main application P 21 37 146.0-52 of chloride in body fluids under Ver 5 is a method of the type mentioned above, in which the solution of an almost colorless mercury is used as a complexing agent for Hg (II) ions a soluble silver complex and another heavy metal salt of diethyldithiocarbamic acid and, as a heavy ion, which is used with the complexing agent a deep metal ion, a copper (II) ion,
There is a colored complex, characterized by it · It has now been found that a quck shows that the complexing agent used is tripyrido-io complex with tripyrido-s-triazine (TPTZ) in the presence of $ -triazine (TPTZ). of heavy metal ions a far more responsive one 2. The method according to claim 1, characterized in that represents the reagent for chloride ions as indicated above, that the heavy metal ion is an iron (II) - known combination of mercury rhodanide Ion is used. and ferric ions. Subject of the present 3. Reagent for carrying out the method 15 supplementary application is thus a method of the above according to claim 1, containing a colorless mercury species, in which the complexing agent is trisilver complex salt and a heavy metal using pyrido-s-triazine (TPTZ). Strong myself with the complexing agent a deeply colored, diluted serum, such as that used for flame complexes there, characterized in that it can be used photometric, are suitable for contains tripyrido-s-triazine as complexing agent, ao chloride determination according to the invention Process, since even these provide a sufficiently high color intensity. Heavy metal ions are iron (II) ions are preferably used. In contrast to mercury rhodanide, which in solution shows signs of decomposition over time The invention relates to a method for the photographic deposition of sulfur, is the thermal determination of chloride in body-based reagent over years of undecomposed liquids using the solution of a bar. This is all the more surprising since ferrous ions of almost colorless mercury complex and one of them tend to slightly oxidize and then with TPTZ their heavy metal ions, which would no longer give any color with the complex,
former gives a deeply colored complex. The following example explains the invention: The known color reagents on copper (II) -. . salts: oxalic acid-bis-cyclohexylidene hydrazide) (= Cu example prizon) and dithiooxamide (= rubean hydrogen) 1st reagent stock solution, form in the presence of copper (II) salts with 35 5 · lü "~ ³ M TPTZ, Hg (II) ions colorless complexes, but their 5 · 10 " ³ M Merkuri nitrate, Dissociation constant so low that the addition of 5 · 10 " ³ M ferro-ammonium sulfate, of chloride ions not to form HgCl ₂ and dissolved in 10% ethanol. leads to the release of the complexing agent and thus this stock solution is 1:10 for use no colored copper complex is formed either 40 diluted, it is durable in the diluted state, can. 2. Standard 100 meq / liter A method is known, according to which one mercury rhodanide in the presence of iron (UI) ions method
used as a reagent for the determination of chloride. We add 0.05 ml of serum to each 2.0 ml of reagent, and the essential thing here is that 0.05 ml of standard (both diluted _{1:20) are added by the formation of HgCl 2 45.} Rhodanide ions are released, which allow the formation After mixing the sample against the reagent of red iron (III) rhodanide staining. Ge as a blank value at 560 to 600 nm in the photometer. The red color can be measured photometrically. The calculation is made according to the form:
and is then a measure of the converted chloride. In principle there is the possibility of such analysis. 5 - ^ meq / liter chloride,
crowding reactions when it is the location of the dissocia- extinction Sundard