DE202004009793U1 - Biological reaction chamber for e.g. micro-arrays, microtitration plates and slides, includes permanent magnets in lid and walls - Google Patents
Biological reaction chamber for e.g. micro-arrays, microtitration plates and slides, includes permanent magnets in lid and walls Download PDFInfo
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- DE202004009793U1 DE202004009793U1 DE202004009793U DE202004009793U DE202004009793U1 DE 202004009793 U1 DE202004009793 U1 DE 202004009793U1 DE 202004009793 U DE202004009793 U DE 202004009793U DE 202004009793 U DE202004009793 U DE 202004009793U DE 202004009793 U1 DE202004009793 U1 DE 202004009793U1
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
- B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
- B01L3/508—Containers for the purpose of retaining a material to be analysed, e.g. test tubes rigid containers not provided for above
- B01L3/5085—Containers for the purpose of retaining a material to be analysed, e.g. test tubes rigid containers not provided for above for multiple samples, e.g. microtitration plates
- B01L3/50851—Containers for the purpose of retaining a material to be analysed, e.g. test tubes rigid containers not provided for above for multiple samples, e.g. microtitration plates specially adapted for heating or cooling samples
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
- B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
- B01L3/508—Containers for the purpose of retaining a material to be analysed, e.g. test tubes rigid containers not provided for above
- B01L3/5085—Containers for the purpose of retaining a material to be analysed, e.g. test tubes rigid containers not provided for above for multiple samples, e.g. microtitration plates
- B01L3/50853—Containers for the purpose of retaining a material to be analysed, e.g. test tubes rigid containers not provided for above for multiple samples, e.g. microtitration plates with covers or lids
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
- B01J2219/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
- B01J2219/00274—Sequential or parallel reactions; Apparatus and devices for combinatorial chemistry or for making arrays; Chemical library technology
- B01J2219/00277—Apparatus
- B01J2219/00279—Features relating to reactor vessels
- B01J2219/00281—Individual reactor vessels
- B01J2219/00283—Reactor vessels with top opening
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
- B01J2219/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
- B01J2219/00274—Sequential or parallel reactions; Apparatus and devices for combinatorial chemistry or for making arrays; Chemical library technology
- B01J2219/00277—Apparatus
- B01J2219/00279—Features relating to reactor vessels
- B01J2219/00306—Reactor vessels in a multiple arrangement
- B01J2219/00313—Reactor vessels in a multiple arrangement the reactor vessels being formed by arrays of wells in blocks
- B01J2219/00315—Microtiter plates
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
- B01J2219/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
- B01J2219/00274—Sequential or parallel reactions; Apparatus and devices for combinatorial chemistry or for making arrays; Chemical library technology
- B01J2219/00277—Apparatus
- B01J2219/00279—Features relating to reactor vessels
- B01J2219/00331—Details of the reactor vessels
- B01J2219/00333—Closures attached to the reactor vessels
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
- B01J2219/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
- B01J2219/00274—Sequential or parallel reactions; Apparatus and devices for combinatorial chemistry or for making arrays; Chemical library technology
- B01J2219/00277—Apparatus
- B01J2219/00479—Means for mixing reactants or products in the reaction vessels
- B01J2219/00484—Means for mixing reactants or products in the reaction vessels by shaking, vibrating or oscillating of the reaction vessels
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
- B01J2219/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
- B01J2219/00274—Sequential or parallel reactions; Apparatus and devices for combinatorial chemistry or for making arrays; Chemical library technology
- B01J2219/00583—Features relative to the processes being carried out
- B01J2219/00603—Making arrays on substantially continuous surfaces
- B01J2219/00605—Making arrays on substantially continuous surfaces the compounds being directly bound or immobilised to solid supports
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
- B01J2219/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
- B01J2219/00274—Sequential or parallel reactions; Apparatus and devices for combinatorial chemistry or for making arrays; Chemical library technology
- B01J2219/00583—Features relative to the processes being carried out
- B01J2219/00603—Making arrays on substantially continuous surfaces
- B01J2219/00659—Two-dimensional arrays
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
- B01J2219/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
- B01J2219/00274—Sequential or parallel reactions; Apparatus and devices for combinatorial chemistry or for making arrays; Chemical library technology
- B01J2219/00718—Type of compounds synthesised
- B01J2219/0072—Organic compounds
- B01J2219/00722—Nucleotides
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
- B01J2219/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
- B01J2219/00274—Sequential or parallel reactions; Apparatus and devices for combinatorial chemistry or for making arrays; Chemical library technology
- B01J2219/00718—Type of compounds synthesised
- B01J2219/0072—Organic compounds
- B01J2219/00725—Peptides
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L2200/00—Solutions for specific problems relating to chemical or physical laboratory apparatus
- B01L2200/02—Adapting objects or devices to another
- B01L2200/025—Align devices or objects to ensure defined positions relative to each other
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L2300/00—Additional constructional details
- B01L2300/04—Closures and closing means
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L2300/00—Additional constructional details
- B01L2300/06—Auxiliary integrated devices, integrated components
- B01L2300/0627—Sensor or part of a sensor is integrated
- B01L2300/0636—Integrated biosensor, microarrays
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L2300/00—Additional constructional details
- B01L2300/08—Geometry, shape and general structure
- B01L2300/0809—Geometry, shape and general structure rectangular shaped
- B01L2300/0829—Multi-well plates; Microtitration plates
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L9/00—Supporting devices; Holding devices
- B01L9/52—Supports specially adapted for flat sample carriers, e.g. for plates, slides, chips
- B01L9/523—Supports specially adapted for flat sample carriers, e.g. for plates, slides, chips for multisample carriers, e.g. used for microtitration plates
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M23/00—Constructional details, e.g. recesses, hinges
- C12M23/02—Form or structure of the vessel
- C12M23/12—Well or multiwell plates
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M23/00—Constructional details, e.g. recesses, hinges
- C12M23/38—Caps; Covers; Plugs; Pouring means
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M23/00—Constructional details, e.g. recesses, hinges
- C12M23/46—Means for fastening
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- Health & Medical Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Analytical Chemistry (AREA)
- General Health & Medical Sciences (AREA)
- Hematology (AREA)
- Clinical Laboratory Science (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Apparatus Associated With Microorganisms And Enzymes (AREA)
- Automatic Analysis And Handling Materials Therefor (AREA)
Abstract
Description
Die Erfindung betrifft eine Reaktionskammer zum Prozessieren, insbesondere Hybridisieren, von biologischen Proben, insbesondere solchen auf Mikroarrays, Mikrotiterplatten und Objektträgern für zeltbiologische Anwendungen.The invention relates to a reaction chamber for Processing, in particular hybridizing, of biological samples, especially those on microarrays, microtiter plates and slides for tent biological Applications.
Mikroarrays nehmen eine Schlüsselstellung in der modernen Biowissenschaft ein. Unter Mikroarrays sind Objektträger aus verschieden Substraten wie z.B. Glas oder Kunststoffe zu verstehen, auf denen Spots von DNA oder RNA – Oligonukleotiden, Enzymen, Proteinen usw. immobilisiert werden können. Die Spots sind in einem zweidimensionalen Gitter auf dem Substrat angeordnet und in der Regel immobilisiert. Anschließend wird der Mikroarray hybridisiert, d.h. es werden biologische Proben, die häufig mit Fluoreszenzmarkern markiert sind, auf dem Mikroarray aufgebracht und Reaktionsbedingungen erzeugt, die das Binden der Proben an die Spots auf dem Array unterstützen.Microarrays play a key role modern bioscience. Slides are made from microarrays different substrates such as Understanding glass or plastics on which spots of DNA or RNA - oligonucleotides, enzymes, Proteins, etc. can be immobilized. The spots are in one two-dimensional grid arranged on the substrate and in the Usually immobilized. Subsequently the microarray is hybridized, i.e. biological samples who often with Fluorescent markers are marked, applied to the microarray and reaction conditions are created that bind the samples to the Support spots on the array.
Mikroarrays haben sich in der Forschung als erfolgreiches Werkzeug etabliert. Zum Hybridisieren der Arrays sind mehrere Vorrichtungen und Verfahrensweisen bekannt. Eine Methode um eine Hybridisierungsreaktion durchzuführen ist, nach dem Aufbringen der biologischen Probe ein Deckglas auf den Mikroarray aufzulegen. Das Deckglas verteilt die Probenlösung. Außerdem verteilt sich die Probenlösung aufgrund von Kapillarkräften gut unter dem Deckglas. Nachteilig wirken sich bei solchen Ausführungen die starke Haftung des Deckglases am Mikroarray, der mögliche Einschluss von Luftblasen und die geringe Reproduzierbarkeit aus.Microarrays have been used in research established as a successful tool. For hybridizing the arrays Several devices and procedures are known. A method to perform a hybridization reaction after application place a cover slip on the microarray of the biological sample. The cover glass distributes the sample solution. In addition, the sample solution spreads due to of capillary forces well under the coverslip. Such designs have a disadvantageous effect the strong adhesion of the cover slip to the microarray, the possible inclusion from air bubbles and the low reproducibility.
Zur Vermeidung dieses Nachteils werden nach dem Stand der Technik sogenannte Hybridisierungskammern eingesetzt, bei denen Mikro-Arrays in die Kammer eingelegt sind, die mit einem Reaktanden überschichtet und anschließend mit einem Deckel verschlossen werden.To avoid this disadvantage, after so-called hybridization chambers used in the prior art, where micro arrays are placed in the chamber, which is covered with a reactant and subsequently be closed with a lid.
Vorliegender Erfindung am nächsten kommt dabei
ein in
Ähnliche Nybridisierungskammern wie vorstehend beschrieben, werden auch für ein oder mehrere Mikroarrays von Unternehmen wie z.B. Scienion AG, HTI-Bio-X GmbH angeboten. Auch dort wird die Kammer durch einen Boden und einen Deckel ausgebildet. Im Boden ist eine Aufnahme für das Mikroarray vorgesehen. Weiterhin wird der Boden von einer Gummidichtung umfasst, welche den gesamten Array umgibt. Der Deckel wird auf den Dichtungsring aufgesetzt und mittels vier Schrauben an dem Boden befestigt. Dadurch bildet sich zwischen Deckel, Dichtung und Boden eine luftdicht abgeschlossene Kammer aus, in der sich die Mikroarrays befinden.Similar Hybridization chambers as described above are also used for one or multiple microarrays from companies such as Scienion AG, HTI-Bio-X GmbH offered. There too the chamber is covered by a floor and formed a lid. In the bottom is a holder for the microarray intended. Furthermore, the bottom is covered by a rubber seal, which surrounds the entire array. The lid is placed on the sealing ring and attached to the floor with four screws. This forms there is an airtight seal between the lid, seal and base Chamber in which the microarrays are located.
Zur optimalen Durchführung einer Hybridisierungsreaktion werden die oben beschriebenen Hybridisierungskammern in ein temperiertes Tauchbad gelegt. Der Temperaturbereich liegt je nach angewandeten Protokoll zwischen 10° C und 100° C. Durch die Temperierung entsteht in den Kammern bei Abkühlung ein Unterdruck. Dieser hält den Deckel auch nach dem Lösen aller Befestigungen fest mit dem Boden verbunden. Zum Lösen des Deckels muss eine angemessene Kraft zum seitlichen Abziehen oder eine größere Kraft zum Abheben des Deckels aufgebracht werden. Dadurch kommt es oft zum Vergießen der enthaltenen Flüssigkeit. Des Weiteren kann es durch seitliches Abziehen zur Verletzung der Dichtung kommen. Die darüber hinaus notwendige Kraft zum Öffnen des Deckels ist für den Anwender hinderlich. Ein weiterer Nachteil ist die geringe Anzahl der gleichzeitig prozessierbaren Mikroarrays, Mikrotiterplatten oder Objektträger für zeltbiologische oder Mikroarray-Anwendungen. Die Art der Vorrichtungskonzeption bekannter und zu vorliegender Erfindung vergleichbarer Lösungen gewährleistet weiterhin nicht die Möglichkeit der Durchmischung der biologischen Probe auf dem Array. Dadurch sind geringere Signalstärken bei der Auswertung der einzelnen Experimente zu erwarten. All die bekannten Vorrichtungen für den speziellen Verwendungszweck sind eher für einen kleineren Labormaßstab und Handversuche konzipiert.For optimal execution of a Hybridization reaction become the hybridization chambers described above placed in a tempered immersion bath. The temperature range is depending on the protocol used, between 10 ° C and 100 ° C in the chambers when cooling a negative pressure. This lasts the lid even after loosening all fastenings firmly connected to the ground. To solve the Lid must have adequate pulling force or sideways pull a greater force be applied to lift off the lid. This often leads to Shed the liquid it contains. Furthermore, pulling it sideways can injure the Seal come. The one above force necessary to open the lid is for hinders the user. Another disadvantage is the small number of simultaneously processable microarrays, microtiter plates or slide for tent biological or microarray applications. The type of device design Known and comparable solutions to the present invention further guarantees not the possibility the mixing of the biological sample on the array. This makes fewer signal strength to be expected when evaluating the individual experiments. All the known devices for the special purpose is more for a smaller laboratory scale and manual tests designed.
Aufgabe der Erfindung ist es, eine Reaktionskammer zum Prozessieren von biologischen Proben zu schaffen, die kraft- und materialschonend und unkompliziert geöffnet werden kann und die gleichzeitig einen höheren Probendurchsatz unter Verwendung handelsüblicher Geräte zur Prozessierung von Mikrotiterplatten, wie z.B. Rütteltische, ermöglicht.The object of the invention is a Create reaction chamber for processing biological samples, that are easy and effortless to open can and at the same time a higher sample throughput Use commercially available equipment for processing microtiter plates, e.g. vibrating tables, allows.
Diese Aufgabe wird mit einer Reaktionskammer mit den Merkmalen des Anspruches 1 gelöst. Vorteilhafte Weiterbildungen sind durch die Unteransprüche erfasst.This task is done with a reaction chamber solved with the features of claim 1. Advantageous further training are by the subclaims detected.
Eine Reaktionskammer gemäß vorliegender Erfindung besitzt einen Reaktionsraum, in denen Halter für Objektträger und Mikroarrays oder Mikrotiterplatten oder Halter für Reaktionstubes eingesetzt werden können. Im Bodenbereich des Reaktionsraumes ist zumindest eine Vertiefung zur Aufnahme von Flüssigkeiten eingelassen. Ferner besitzt die Reaktionskammer einen Deckel der auf dem Reaktionsraum auflegbar und mit diesem verbindbar ist. Zwischen Reaktionskammer und Deckel ist eine Dichtung angebracht. Des Weiteren ist die Reaktionskammer als auch der Deckel mit magnetischen Baugruppen versehen, die so angebracht sind, dass ihre Kräfte entgegengesetzt wirken. Reaktionskammer und Deckel werden durch geeignete Verschlussmittel, z.B. Schrauben, miteinander verbunden. So entsteht ein abgeschlossener Reaktionsraum, der den Inhalt vor Einflüssen von außen schützt.A reaction chamber according to the present invention has a reaction space in which holders for slides and microarrays or microtiter plates or holders for reaction tubes can be used. In the bottom area of the reaction space, at least one recess for receiving liquids is embedded. Furthermore, the reaction chamber has a lid which can be placed on and connected to the reaction space. A seal is attached between the reaction chamber and the lid. Furthermore, the reaction chamber and the lid are provided with magnetic assemblies which are attached in such a way that their forces act in opposite directions. Reaction chamber and Lids are connected to one another using suitable locking means, for example screws. This creates a closed reaction space that protects the content from external influences.
Vorteilhaft wirkt sich das Abstoßen der magnetisierten Bauteile auf das Öffnen des Deckels aus. Nach der Hybridisierung lässt er sich trotz herrschenden Unterdrucks kraft- und materialschonend und unkompliziert von dem Reaktionsbehälter entfernen.Repelling has an advantageous effect magnetized components on opening the lid. After hybridization, it can be used despite the prevailing Vacuum is gentle on energy and materials and uncomplicated reaction vessel remove.
Unter der Reaktionskammer sind weiterhin Füße, bevorzugt aus magnetischen Materialien vorgesehen. Diese sind so angebracht, dass die Reaktionskammer auf handelsübliche Geräte für Systeme im Mikrotiterplatten-Format, wie z.B. Mikrotiterplatten-Schüttler oder Thermoschüttler, aufgestellt und über die Füße mechanisch und/oder auch über die wirkende Magnetkraft fixiert werden kann. Vorteilig wirkt sich das Schütteln des Reaktionsbehälters auf das gleichmäßige Verteilen der biologischen Probe auf dem Mikroarray aus. Dies führt zu einer höheren Bindungsanzahl auf dem Array.Feet are also preferred under the reaction chamber made of magnetic materials. These are attached that the reaction chamber on commercially available devices for systems in microtiter plate format, such as. Microplate shaker or thermal shaker, set up and over the feet mechanically and / or also about the acting magnetic force can be fixed. Has an advantageous effect the shaking of the reaction container on the even distribution the biological sample on the microarray. This leads to a higher number of bonds on the array.
Die Erfindung wird beispielhaft an Hand nachstehender schematischer Zeichnungen näher erläutert. Es zeigen:The invention is exemplary Hand the following schematic drawings explained in more detail. Show it:
In die Oberkante des Reaktionsbehälters
Im Beispiel sind auf den beiden Querseiten des
Deckels
Im Rahmen der Erfindung ist weiterhin
die Anordnung von Füßen
Im Rahmen der Erfindung ist es weiterhin vorteilhaft,
die gegenüberliegenden
Längsseiten
der Außenwandungen
des Reaktionsbehälters
- 11
- Reaktionsbehälterreaction vessel
- 22
- Reaktionsraumreaction chamber
- 33
- Halterungbracket
- 44
- Eingriffmuldenengagement troughs
- 55
- Dichtungpoetry
- 66
- Bodenground
- 77
- Vertiefungdeepening
- 88th
- Deckelcover
- 99
- Bohrungendrilling
- 1010
- Gewindebohrungenthreaded holes
- 1111
- erste Dauermagnetefirst permanent magnets
- 811811
- zweite Dauermagnetesecond permanent magnets
- 1212
- Füßefeet
- 1313
- BodenunterseiteBottom underside
- 1414
- Verschlussmittel (Schrauben)closure means (Screws)
- 1515
- Mikrotiterplattemicrotiter plate
- 1616
- Griffstück (-mulde)Handle (recess)
Claims (9)
Priority Applications (1)
Application Number | Priority Date | Filing Date | Title |
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DE202004009793U DE202004009793U1 (en) | 2004-06-21 | 2004-06-21 | Biological reaction chamber for e.g. micro-arrays, microtitration plates and slides, includes permanent magnets in lid and walls |
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
DE202004009793U DE202004009793U1 (en) | 2004-06-21 | 2004-06-21 | Biological reaction chamber for e.g. micro-arrays, microtitration plates and slides, includes permanent magnets in lid and walls |
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Publication Number | Publication Date |
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DE202004009793U1 true DE202004009793U1 (en) | 2004-08-19 |
Family
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Application Number | Title | Priority Date | Filing Date |
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DE202004009793U Expired - Lifetime DE202004009793U1 (en) | 2004-06-21 | 2004-06-21 | Biological reaction chamber for e.g. micro-arrays, microtitration plates and slides, includes permanent magnets in lid and walls |
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Cited By (4)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US20150233815A1 (en) * | 2012-08-09 | 2015-08-20 | Tecan Trading Ag | Microplate Reader with Lid Lifter for Microplates |
CN109153019A (en) * | 2016-05-18 | 2019-01-04 | 米兰综合工科大学 | device for cell culture |
WO2019014541A3 (en) * | 2017-07-13 | 2019-03-07 | Greiner Bio-One North America, Inc. | Culture plates for imaging |
US11351554B2 (en) | 2017-01-30 | 2022-06-07 | 4D Lifetec Ag | Carrier plate for laboratory devices |
-
2004
- 2004-06-21 DE DE202004009793U patent/DE202004009793U1/en not_active Expired - Lifetime
Cited By (8)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US20150233815A1 (en) * | 2012-08-09 | 2015-08-20 | Tecan Trading Ag | Microplate Reader with Lid Lifter for Microplates |
US9568414B2 (en) * | 2012-08-09 | 2017-02-14 | Tecan Trading Ag | Microplate reader with lid lifter for microplates |
CN109153019A (en) * | 2016-05-18 | 2019-01-04 | 米兰综合工科大学 | device for cell culture |
CN109153019B (en) * | 2016-05-18 | 2021-09-24 | 米兰综合工科大学 | Device for cell culture |
US11351554B2 (en) | 2017-01-30 | 2022-06-07 | 4D Lifetec Ag | Carrier plate for laboratory devices |
WO2019014541A3 (en) * | 2017-07-13 | 2019-03-07 | Greiner Bio-One North America, Inc. | Culture plates for imaging |
US20210079329A1 (en) * | 2017-07-13 | 2021-03-18 | Greiner Bio-One North America, Inc. | Culture plates for imaging |
US12049612B2 (en) | 2017-07-13 | 2024-07-30 | Greiner Bio-One North America, Inc. | Culture plates for imaging |
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R207 | Utility model specification |
Effective date: 20040923 |
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R150 | Term of protection extended to 6 years |
Effective date: 20070628 |
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R151 | Term of protection extended to 8 years |
Effective date: 20100719 |
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R158 | Lapse of ip right after 8 years |
Effective date: 20130101 |