DE19515578A1 - Process for selection of strains of bacteria from natural population - Google Patents

Process for selection of strains of bacteria from natural population

Info

Publication number
DE19515578A1
DE19515578A1 DE1995115578 DE19515578A DE19515578A1 DE 19515578 A1 DE19515578 A1 DE 19515578A1 DE 1995115578 DE1995115578 DE 1995115578 DE 19515578 A DE19515578 A DE 19515578A DE 19515578 A1 DE19515578 A1 DE 19515578A1
Authority
DE
Germany
Prior art keywords
bacteria
culture medium
population
substrate
strains
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Withdrawn
Application number
DE1995115578
Other languages
German (de)
Inventor
Guenter Dr Dreyer
Manfred Prof Dr Ringpfeil
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Biopract GmbH
Original Assignee
Biopract GmbH
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Biopract GmbH filed Critical Biopract GmbH
Priority to DE1995115578 priority Critical patent/DE19515578A1/en
Publication of DE19515578A1 publication Critical patent/DE19515578A1/en
Withdrawn legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor

Abstract

Parts of a culture medium are withdrawn discontinuously or continuously from a natural mixed bacteria population cultivated on a complex substrate and the bacteria found in them are killed or otherwise removed. The remaining culture medium is returned to the cultivation vessel. The procedure is repeated until the population is reduced to one to few strains of bacteria. Pref. a substrate is used whose constituents can be rapidly biologically decomposed. Bacteria are killed using ultrasound and sepn. is done by filtration.

Description

Die Erfindung betrifft ein neues Selektionsverfahren für Bakterienstämme. Es ist überall dort anwendbar, wo aus einer natürlichen Bakterienpopulation leistungsfähige Stämme selektiert werden sollen. Bevorzugte Anwendungsgebiete sind die mikrobiologische Industrie und der Umweltschutz.The invention relates to a new selection process for Bacterial strains. It can be used wherever one natural strains of powerful bacteria should be selected. Preferred areas of application are microbiological industry and environmental protection.

Für die Suche nach Mikroorganismen mit bestimmten Eigenschaften, etwa mit der Fähigkeit zum Abbau von speziellen Schadstoffen, werden weltweit große Kapazitäten eingesetzt. So sucht man seit längerem nach teerölabbauenden Bakterien, wobei die Schwierigkeit darin besteht, insbesondere die enthaltenen polycyclischen Stoffe (vierkernig und größer) mikrobiell abzubauen.For the search for microorganisms with certain Properties, such as the ability to break down special ones Pollutants, large capacities are used worldwide. So has been looking for tar oil-degrading bacteria for a long time, whereby the difficulty is, especially the ones contained polycyclic substances (quad-core and larger) microbial dismantle.

Die Erfindung hat das Ziel, ein Verfahren zu entwickeln, mit dem in natürlichen Populationen enthaltene Bakterien mit gewünschten Eigenschaften selektiert werden können.The invention aims to develop a method with which bacteria contained in natural populations with desired Properties can be selected.

Die Erfindung wird nach Anspruch 1 realisiert, die Unteransprüche 2-6 sind Vorzugsvarianten. Eine für die Durchführung des Verfahrens besonders geeignete Vorrichtung ist in Abb. 1 schematisch dargestellt.The invention is implemented according to claim 1, subclaims 2-6 are preferred variants. A device which is particularly suitable for carrying out the method is shown schematically in FIG. 1.

Die Erfindung wird nachstehend durch ein Ausführungsbeispiel näher erläutert.The invention is illustrated below by an embodiment explained in more detail.

Versuchsaufbau:
Es wurde eine Versuchsanordnung gewählt bestehend aus einem Laborbioreaktor mit angeschlossenem Abluftanalysator für CO₂ und O₂ und Rechnersteuerung.Experiment setup:
A test arrangement was selected consisting of a laboratory bioreactor with a connected exhaust air analyzer for CO₂ and O₂ and computer control.

Zur Sicherung einer gleichbleibend hohen Qualität der Teerölemulsion während der Fermentationsdauer wurde der Fermentorinhalt mittels einer Peristaltikpumpe mit zwei Pumpen­ köpfen kontinuierlich durch eine Ultraschalldurchflußzelle geleitet. Der Versuchsaufbau ist in Abb. 1 dargestellt.To ensure a consistently high quality of the tar oil emulsion during the fermentation period, the fermentor content was continuously passed through an ultrasonic flow cell by means of a peristaltic pump with two pump heads. The experimental setup is shown in Fig. 1.

Claims (6)

1. Verfahren zur Selektion von Bakterien, dadurch gekennzeichnet, daß
  • - man eine natürliche Bakterienmischpopulation auf einem komplexen Substrat kultiviert,
  • - diskontinuierlich oder kontinuierlich Teile des Kulturmediums entnimmt,
  • - die darin befindlichen Bakterien abtötet oder anderweitig entfernt
  • - das verbleibende Kulturmedium in das Kultivierungsgefäß zurückführt und
  • - diesen Vorgang so oft wiederholt, bis die eingesetzte Population auf einen bis wenige Bakterienstämme reduziert ist.
1. A method for the selection of bacteria, characterized in that
  • one cultivates a natural mixed bacterial population on a complex substrate,
  • - discontinuously or continuously removes parts of the culture medium,
  • - kills or otherwise removes the bacteria inside
  • - the remaining culture medium is returned to the culture vessel and
  • - Repeat this process until the population used is reduced to one to a few strains of bacteria.
2. Verfahren nach Anspruch 1, dadurch gekennzeichnet, daß ein Substrat eingesetzt wird, dessen Bestandteile unterschiedlich schnell biologisch abbaubar sind.2. The method according to claim 1, characterized in that a Substrate is used, the components of which differ are rapidly biodegradable. 3. Verfahren nach Anspruch 1 und 2, dadurch gekennzeichnet, daß die kontinuierliche Entnahme des Kulturmediums mittels einer Durchflußvorrichtung erfolgt.3. The method according to claim 1 and 2, characterized in that the continuous removal of the culture medium by means of a Flow device takes place. 4. Verfahren nach Anspruch 1-3, dadurch gekennzeichnet, daß die Abtötung der Bakterien durch Ultraschall erfolgt.4. The method according to claim 1-3, characterized in that the The bacteria are killed by ultrasound. 5. Verfahren nach Anspruch 1-3, dadurch gekennzeichnet, daß die Entfernung der Bakterien durch Filtration erfolgt.5. The method according to claim 1-3, characterized in that the The bacteria are removed by filtration. 6. Verfahren nach Anspruch 1-5, dadurch gekennzeichnet, daß die Bakterienpopulation während des Verfahrens regelmäßig analysiert wird.6. The method according to claim 1-5, characterized in that the Bacterial population analyzed regularly during the procedure becomes.
DE1995115578 1995-04-27 1995-04-27 Process for selection of strains of bacteria from natural population Withdrawn DE19515578A1 (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
DE1995115578 DE19515578A1 (en) 1995-04-27 1995-04-27 Process for selection of strains of bacteria from natural population

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
DE1995115578 DE19515578A1 (en) 1995-04-27 1995-04-27 Process for selection of strains of bacteria from natural population

Publications (1)

Publication Number Publication Date
DE19515578A1 true DE19515578A1 (en) 1996-10-31

Family

ID=7760547

Family Applications (1)

Application Number Title Priority Date Filing Date
DE1995115578 Withdrawn DE19515578A1 (en) 1995-04-27 1995-04-27 Process for selection of strains of bacteria from natural population

Country Status (1)

Country Link
DE (1) DE19515578A1 (en)

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2010072818A2 (en) 2008-12-23 2010-07-01 Bekon Energy Technologies Gmbh & Co. Kg Gas treatment appliance and gas treatment process

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2010072818A2 (en) 2008-12-23 2010-07-01 Bekon Energy Technologies Gmbh & Co. Kg Gas treatment appliance and gas treatment process
DE102008062811A1 (en) * 2008-12-23 2010-11-04 Bekon Energy Technologies Gmbh & Co. Kg Gas treatment device and gas treatment process

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