DE1180166B - Broadband pipette, especially for paper and thin layer chromatography as well as for electrophoresis, for applying liquids to sorbent layers - Google Patents
Broadband pipette, especially for paper and thin layer chromatography as well as for electrophoresis, for applying liquids to sorbent layersInfo
- Publication number
- DE1180166B DE1180166B DEST18647A DEST018647A DE1180166B DE 1180166 B DE1180166 B DE 1180166B DE ST18647 A DEST18647 A DE ST18647A DE ST018647 A DEST018647 A DE ST018647A DE 1180166 B DE1180166 B DE 1180166B
- Authority
- DE
- Germany
- Prior art keywords
- broadband
- pipette
- plates
- electrophoresis
- paper
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N27/00—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means
- G01N27/26—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating electrochemical variables; by using electrolysis or electrophoresis
- G01N27/416—Systems
- G01N27/447—Systems using electrophoresis
- G01N27/44704—Details; Accessories
- G01N27/44743—Introducing samples
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
- G01N30/90—Plate chromatography, e.g. thin layer or paper chromatography
- G01N30/91—Application of the sample
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- General Health & Medical Sciences (AREA)
- Analytical Chemistry (AREA)
- Biochemistry (AREA)
- Physics & Mathematics (AREA)
- General Physics & Mathematics (AREA)
- Immunology (AREA)
- Pathology (AREA)
- Molecular Biology (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Electrochemistry (AREA)
- Devices For Use In Laboratory Experiments (AREA)
Description
Breitband-Pipette, insbesondere für Papier- und Dünnschichtchromatographie sowie für Elektrophorese, zum Auftragen von Flüssigkeiten auf Sorptionsschichten In vielen Fällen der Laboratoriumstechnik, insbesondere bei der Papier- und Dünnschichtchromatographie sowie bei der Elektrophorese, ergibt sich die Notwendigkeit, flüssige Mittel in gleichmäßigen Streifen auf Sorptionsschichten aufzutragen.Broadband pipette, especially for paper and thin layer chromatography as well as for electrophoresis, for applying liquids to sorbent layers In many cases of laboratory technology, especially paper and thin-layer chromatography as well as in electrophoresis, there arises the need to put liquid resources in to apply even strips on sorbent layers.
Diesem Erfordernis wird nach der Erfindung durch eine Breitband-Pipette nachgekommen, die aus zwei aufeinandergelegt gehaltenen Platten, insbesondere Glasplatten, mit einander zu laufend etwas angeschärften Auftragskanten besteht, von denen die eine an der Innenseite mit einer Längsriffelung und in einiger Entfernung von der Auftragskante mit einer Querrille versehen ist. Die beiden Platten sind dabei zweckmäßig abziehbar von einem Heftgriff aufgenommen, dessen Aufnahmeschlitz mit den sicheren Anlageschluß der beiden Platten gewährleistenden Klemmfedern versehen ist. According to the invention, this requirement is met by a broadband pipette made up of two plates, in particular glass plates, held one on top of the other, with each other to continuously somewhat sharpened order edges, of which the one on the inside with a longitudinal corrugation and at some distance from the Application edge is provided with a transverse groove. The two plates are useful here detachable from a handle, its slot with the safe The closure of the two plates ensuring clamping springs is provided.
Die neue Breitband-Pipette nach vorliegender Erfindung saugt beim Eintauchen der Auftragkante in die aufzutragende Flüssigkeit zufolge der Kapillarwirkung der Riffelung die Flüssigkeit etwa bis zur Querrille an und gibt diese dann beim Aufsetzen auf die Sorptionsschicht wieder ab. Da die erfindungsgemäße Breitband-Pipette aus einzelnen Teilen besteht, ist sie leicht herzustellen und nach Gebrauch leicht zu reinigen. Die beiden Platten sind in dem Griffheft dicht aneinanderliegend gehalten, so daß eine gleichmäßige Abgabe der Flüssigkeit gewährleistet ist. Die Riffelung und die Querrille können je nach der Beweglichkeit der aufzunehmenden und wieder abzugebenden Flüssigkeit verschieden fein oder groß gehalten sein. The new broadband pipette according to the present invention sucks when Immersion of the application edge in the liquid to be applied due to the capillary action the corrugation to the liquid approximately up to the transverse groove and then gives it to the Put on the sorption layer again. Since the broadband pipette according to the invention consists of individual parts, it is easy to manufacture and light after use to clean. The two plates are held close together in the handle, so that an even delivery of the liquid is guaranteed. The corrugation and the transverse groove can vary depending on the mobility of the recorded and re-recorded The liquid to be dispensed can be kept different in fine or large.
Das neue Gerät ist nachstehend an Hand der Zeichnung näher erläutert. Es zeigt Fig. 1 im Schaubild die beiden Platten in voneinander abgezogener Lage, F i g. 2 eine Draufsicht zu F i g. 1 und Fig. 3 einen Schnitt durch das Griffheft mit den zum Einschieben bereiten Platten. The new device is explained in more detail below with reference to the drawing. It shows Fig. 1 in a diagram, the two plates in a peeled off position, F i g. 2 is a plan view of FIG. 1 and 3 show a section through the handle with the plates ready for insertion.
Die Pipette besteht aus zwei gleich großen Platten, vorzugsweise Glasplatten, 1, 2, von denen die eine glatt geschliffen ist, während die andere auf der Innenseite mit einer Längsriffelung 3 und einer Querrille 4 versehen ist. The pipette consists of two plates of equal size, preferably Glass plates, 1, 2, one of which is sanded smooth while the other is provided with a longitudinal corrugation 3 and a transverse groove 4 on the inside.
Die unteren Kanten 5, 6 der beiden Platten 1, 2 sind aufeinander zu laufend ziemlich stark angeschärft Bei dicht aufeinandergelegten Platten (Fig. 3) wirkt die Längsriffelung 3 als Kapillaranordnung, die beim Eintauchen der angeschärften Kanten, 6 in eine Flüssigkeit diese ansaugt. Die Querrille 4 dient dabei als obere Begrenzung der in den Kapillaren aufsteigenden Flüssigkeit. The lower edges 5, 6 of the two plates 1, 2 are on top of one another too sharply sharpened when the panels are placed close to each other (Fig. 3) the longitudinal corrugation 3 acts as a capillary arrangement that is sharpened when the Edges, 6 in a liquid this sucks. The transverse groove 4 serves as the upper one Limitation of the liquid rising in the capillaries.
Um den erforderlichen dichten und festen Aneinanderschluß der beiden Platten 1, 2 zu gewährleisten, ist ein mit einem Schlitzloch 8 versehenes Grifiheft 7 vorgesehen, das gegebenenfalls aus zwei miteinander verschraubten Halbschalen bestehen kann. Die Seitenwände des Schlitzloches 8 nehmen Blattfedern 9 auf, die die eingeschobenen Platten 1, 2 in einer Preßlage aneinanderhalten. To achieve the necessary tight and firm connection between the two To ensure plates 1, 2 is a handle booklet provided with a slotted hole 8 7 is provided, which optionally consists of two half-shells screwed together can exist. The side walls of the slot hole 8 take on leaf springs 9, the hold the inserted plates 1, 2 together in a pressed layer.
Nach einer Ausführungsform der erfindungsgemäßen Breitband-Pipette sind die beiden aufeinander zu laufenden Kanten 5, 6 bis zur Spitze auslaufend angeschärft, wogegen nach einer anderen Ausführungsform die Kanten zwar auch etwas angeschärft sind, jedoch nicht bis zur Spitze auslaufend. According to one embodiment of the broadband pipette according to the invention the two edges 5, 6 running towards each other are sharpened to the tip, whereas, according to another embodiment, the edges are also somewhat sharpened are, but not tapering to the tip.
Die Platten 1, 2 können aus beliebigem Material, das gegen die aufzunehmende Flüssigkeit resistent ist, hergestellt sein. So können beide Platten aus Glas bestehen oder aus V2A-Stahl. Nach einer bevorzugten Ausführungsform ist die nicht geriffelte Platte aus durchsichtigem Material, damit der die Breitband-Pipette Handhabende erkennen kann, ob die Flüssigkeit genügend hoch gewandert ist.The plates 1, 2 can be made of any material against the to be recorded Liquid resistant, be made. Both plates can be made of glass or made of V2A steel. According to a preferred embodiment, it is not corrugated Plate made of transparent material so that the person handling the broadband pipette can tell whether the liquid has migrated high enough.
Claims (5)
Priority Applications (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
DEST18647A DE1180166B (en) | 1961-12-06 | 1961-12-06 | Broadband pipette, especially for paper and thin layer chromatography as well as for electrophoresis, for applying liquids to sorbent layers |
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
DEST18647A DE1180166B (en) | 1961-12-06 | 1961-12-06 | Broadband pipette, especially for paper and thin layer chromatography as well as for electrophoresis, for applying liquids to sorbent layers |
Publications (1)
Publication Number | Publication Date |
---|---|
DE1180166B true DE1180166B (en) | 1964-10-22 |
Family
ID=7457934
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
DEST18647A Pending DE1180166B (en) | 1961-12-06 | 1961-12-06 | Broadband pipette, especially for paper and thin layer chromatography as well as for electrophoresis, for applying liquids to sorbent layers |
Country Status (1)
Country | Link |
---|---|
DE (1) | DE1180166B (en) |
Cited By (6)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US3495446A (en) * | 1967-06-19 | 1970-02-17 | Cordis Corp | Applicator for chromatography and electrophoresis |
US3505858A (en) * | 1966-11-25 | 1970-04-14 | Shandon Scient Co Ltd | Applicator means |
US4539182A (en) * | 1983-04-08 | 1985-09-03 | Miles Laboratories, Inc. | Automated reagent blotter |
US4801431A (en) * | 1985-09-13 | 1989-01-31 | Fisher Scientific Company | Slide pair holder and assembly |
WO1997042496A1 (en) * | 1996-05-06 | 1997-11-13 | Helena Laboratories Corporation | A system for the application of samples on a substrate__________ |
EP1338893A2 (en) * | 2002-02-25 | 2003-08-27 | ALFA WASSERMANN S.p.A. | Applicator of a fluid sample on a substrate |
-
1961
- 1961-12-06 DE DEST18647A patent/DE1180166B/en active Pending
Cited By (7)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US3505858A (en) * | 1966-11-25 | 1970-04-14 | Shandon Scient Co Ltd | Applicator means |
US3495446A (en) * | 1967-06-19 | 1970-02-17 | Cordis Corp | Applicator for chromatography and electrophoresis |
US4539182A (en) * | 1983-04-08 | 1985-09-03 | Miles Laboratories, Inc. | Automated reagent blotter |
US4801431A (en) * | 1985-09-13 | 1989-01-31 | Fisher Scientific Company | Slide pair holder and assembly |
WO1997042496A1 (en) * | 1996-05-06 | 1997-11-13 | Helena Laboratories Corporation | A system for the application of samples on a substrate__________ |
EP1338893A2 (en) * | 2002-02-25 | 2003-08-27 | ALFA WASSERMANN S.p.A. | Applicator of a fluid sample on a substrate |
EP1338893A3 (en) * | 2002-02-25 | 2004-02-04 | ALFA WASSERMANN S.p.A. | Applicator of a fluid sample on a substrate |
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