DE10328102A1 - Growing flesh flies on plant-based substrates, used to produce larvae and pupae for promotion of wound healing, eliminates risk of transferring pathogens associated with use of animal-based nutrient media - Google Patents
Growing flesh flies on plant-based substrates, used to produce larvae and pupae for promotion of wound healing, eliminates risk of transferring pathogens associated with use of animal-based nutrient media Download PDFInfo
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- DE10328102A1 DE10328102A1 DE2003128102 DE10328102A DE10328102A1 DE 10328102 A1 DE10328102 A1 DE 10328102A1 DE 2003128102 DE2003128102 DE 2003128102 DE 10328102 A DE10328102 A DE 10328102A DE 10328102 A1 DE10328102 A1 DE 10328102A1
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- 239000000758 substrate Substances 0.000 title claims abstract description 13
- 235000015097 nutrients Nutrition 0.000 title claims description 11
- 241001465754 Metazoa Species 0.000 title claims description 10
- 241000257185 Sarcophagidae Species 0.000 title abstract description 5
- 244000052769 pathogen Species 0.000 title description 3
- 230000029663 wound healing Effects 0.000 title description 3
- 238000000034 method Methods 0.000 claims abstract 12
- 235000013527 bean curd Nutrition 0.000 claims description 9
- 241000255925 Diptera Species 0.000 claims description 7
- 241000196324 Embryophyta Species 0.000 claims description 7
- 235000015099 wheat brans Nutrition 0.000 claims description 7
- 235000010469 Glycine max Nutrition 0.000 claims description 6
- 241000894007 species Species 0.000 claims description 6
- 238000009395 breeding Methods 0.000 claims description 5
- 230000001488 breeding effect Effects 0.000 claims description 5
- 235000013372 meat Nutrition 0.000 claims description 5
- 235000013312 flour Nutrition 0.000 claims description 4
- 241000219745 Lupinus Species 0.000 claims description 3
- 240000008042 Zea mays Species 0.000 claims description 3
- 235000002017 Zea mays subsp mays Nutrition 0.000 claims description 3
- 244000052637 human pathogen Species 0.000 claims description 3
- 241000894006 Bacteria Species 0.000 claims description 2
- 241000257160 Calliphora Species 0.000 claims description 2
- 241000257162 Lucilia <blowfly> Species 0.000 claims description 2
- 241000257149 Phormia Species 0.000 claims description 2
- 241000257190 Sarcophaga <genus> Species 0.000 claims description 2
- 241000209140 Triticum Species 0.000 claims description 2
- 235000021307 Triticum Nutrition 0.000 claims description 2
- 239000000284 extract Substances 0.000 claims description 2
- 229910052500 inorganic mineral Inorganic materials 0.000 claims description 2
- 238000004519 manufacturing process Methods 0.000 claims description 2
- 244000005700 microbiome Species 0.000 claims description 2
- 235000010755 mineral Nutrition 0.000 claims description 2
- 239000011707 mineral Substances 0.000 claims description 2
- 235000013619 trace mineral Nutrition 0.000 claims description 2
- 239000011573 trace mineral Substances 0.000 claims description 2
- 235000013343 vitamin Nutrition 0.000 claims description 2
- 239000011782 vitamin Substances 0.000 claims description 2
- 229940088594 vitamin Drugs 0.000 claims description 2
- 229930003231 vitamin Natural products 0.000 claims description 2
- 241000195493 Cryptophyta Species 0.000 claims 1
- 108010082495 Dietary Plant Proteins Proteins 0.000 claims 1
- 241000233866 Fungi Species 0.000 claims 1
- 235000005824 Zea mays ssp. parviglumis Nutrition 0.000 claims 1
- 235000005822 corn Nutrition 0.000 claims 1
- 239000003102 growth factor Substances 0.000 claims 1
- 235000020374 simple syrup Nutrition 0.000 claims 1
- 206010052428 Wound Diseases 0.000 description 6
- 208000027418 Wounds and injury Diseases 0.000 description 6
- 239000006188 syrup Substances 0.000 description 6
- 235000020357 syrup Nutrition 0.000 description 6
- 241000219310 Beta vulgaris subsp. vulgaris Species 0.000 description 5
- 235000021536 Sugar beet Nutrition 0.000 description 5
- 235000008504 concentrate Nutrition 0.000 description 4
- 239000012141 concentrate Substances 0.000 description 4
- 235000013601 eggs Nutrition 0.000 description 3
- 235000013305 food Nutrition 0.000 description 3
- 241000238631 Hexapoda Species 0.000 description 2
- 241000736227 Lucilia sericata Species 0.000 description 2
- 241000257159 Musca domestica Species 0.000 description 2
- 244000046052 Phaseolus vulgaris Species 0.000 description 2
- 235000010627 Phaseolus vulgaris Nutrition 0.000 description 2
- 102000029797 Prion Human genes 0.000 description 2
- 108091000054 Prion Proteins 0.000 description 2
- 241000304160 Sarcophaga carnaria Species 0.000 description 2
- 235000016383 Zea mays subsp huehuetenangensis Nutrition 0.000 description 2
- 239000000654 additive Substances 0.000 description 2
- 238000011109 contamination Methods 0.000 description 2
- 235000005911 diet Nutrition 0.000 description 2
- 230000037213 diet Effects 0.000 description 2
- 230000035876 healing Effects 0.000 description 2
- 235000009973 maize Nutrition 0.000 description 2
- 239000000463 material Substances 0.000 description 2
- 239000000203 mixture Substances 0.000 description 2
- 230000009340 pathogen transmission Effects 0.000 description 2
- 235000017807 phytochemicals Nutrition 0.000 description 2
- 229930000223 plant secondary metabolite Natural products 0.000 description 2
- 238000002560 therapeutic procedure Methods 0.000 description 2
- 241000251468 Actinopterygii Species 0.000 description 1
- 235000001674 Agaricus brunnescens Nutrition 0.000 description 1
- 241000257163 Calliphora vicina Species 0.000 description 1
- 241000257161 Calliphoridae Species 0.000 description 1
- 244000052363 Cynodon dactylon Species 0.000 description 1
- 241000287828 Gallus gallus Species 0.000 description 1
- 244000068988 Glycine max Species 0.000 description 1
- 241000270322 Lepidosauria Species 0.000 description 1
- 241000257226 Muscidae Species 0.000 description 1
- 241000257186 Phormia regina Species 0.000 description 1
- 235000019764 Soybean Meal Nutrition 0.000 description 1
- 241000700605 Viruses Species 0.000 description 1
- 235000021120 animal protein Nutrition 0.000 description 1
- 230000005540 biological transmission Effects 0.000 description 1
- 238000009835 boiling Methods 0.000 description 1
- 235000014633 carbohydrates Nutrition 0.000 description 1
- 150000001720 carbohydrates Chemical class 0.000 description 1
- 239000003795 chemical substances by application Substances 0.000 description 1
- 239000000701 coagulant Substances 0.000 description 1
- 230000015271 coagulation Effects 0.000 description 1
- 238000005345 coagulation Methods 0.000 description 1
- 239000000470 constituent Substances 0.000 description 1
- 238000001816 cooling Methods 0.000 description 1
- 235000013365 dairy product Nutrition 0.000 description 1
- 238000000605 extraction Methods 0.000 description 1
- 210000003608 fece Anatomy 0.000 description 1
- 238000000855 fermentation Methods 0.000 description 1
- 230000004151 fermentation Effects 0.000 description 1
- 235000011389 fruit/vegetable juice Nutrition 0.000 description 1
- 230000012010 growth Effects 0.000 description 1
- 208000015181 infectious disease Diseases 0.000 description 1
- 239000004615 ingredient Substances 0.000 description 1
- 230000009571 larval growth Effects 0.000 description 1
- 150000002632 lipids Chemical class 0.000 description 1
- 210000004185 liver Anatomy 0.000 description 1
- 235000012054 meals Nutrition 0.000 description 1
- 239000002855 microbicide agent Substances 0.000 description 1
- 210000003205 muscle Anatomy 0.000 description 1
- 230000001338 necrotic effect Effects 0.000 description 1
- 230000017448 oviposition Effects 0.000 description 1
- 239000011120 plywood Substances 0.000 description 1
- 235000015277 pork Nutrition 0.000 description 1
- 238000002360 preparation method Methods 0.000 description 1
- 230000000384 rearing effect Effects 0.000 description 1
- 208000008864 scrapie Diseases 0.000 description 1
- 230000028327 secretion Effects 0.000 description 1
- 235000013322 soy milk Nutrition 0.000 description 1
- 239000004455 soybean meal Substances 0.000 description 1
- 238000003860 storage Methods 0.000 description 1
- 210000001519 tissue Anatomy 0.000 description 1
- 239000013598 vector Substances 0.000 description 1
- 235000013311 vegetables Nutrition 0.000 description 1
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K67/00—Rearing or breeding animals, not otherwise provided for; New or modified breeds of animals
- A01K67/033—Rearing or breeding invertebrates; New breeds of invertebrates
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- Life Sciences & Earth Sciences (AREA)
- Environmental Sciences (AREA)
- Animal Behavior & Ethology (AREA)
- Zoology (AREA)
- Animal Husbandry (AREA)
- Biodiversity & Conservation Biology (AREA)
- Coloring Foods And Improving Nutritive Qualities (AREA)
Abstract
Description
Fliegen legen ihre Eier auf artspezifisch bevorzugten Substraten ab. Die meisten Vertreter der Gruppe der Fleischfliegen (Familien Sarcophagidae und Calliphoridae) nutzen in der Natur faktisch immer Fleisch (z.B. tote Tiere) bzw. tierische oder menschliche Exkremente als Ablagestätten für ihre Eier, weil die darin enthaltenen Nährstoffe offenbar optimal für das Larvenwachstum sind (E. Martini 1946: Medizinische Entomologie, G. Fischer, Jena; W. Jacobs, M. Renner 1988, Biologie und Ökologie der Insekten: G. Fischer, Stuttgart; K. Dettner, W. Peters et al., 1999, Lehrbuch der Entomologie, G. Fischer, Stuttgart). Die Ernährung der Fleischfliegen unterscheidet sich grundlegend von der der saprophagen Fliege Musca domestica und verwandten Arten (die z.B. zur Fam. Muscidae gehören), bei denen die Larven in der Natur auch auf fauligen Pflanzenmaterial wachsen. Vor diesem Hintergrund ist es nicht verwunderlich, dass die weltweit in vielen Laboratorien erfolgenden Zuchten der „Fleischfliegen" tatsächlich ausschließlich unter Verwendung von Fleisch oder tierischen Produkten (Pferdemuskel, Schweineleber etc.) erfolgen. Diese so produzierten Fliegenmaden und adulten Fliegen werden zu verschiedensten Zwecken verwendet, z.B. für wissenschaftliche Untersuchungen, als Futter für Reptilien, als Köder für Fische beim Angeln etc. Bei der Zucht von Vertretern der saprophagen Arten (z.B. Musca domestica) wurden dagegen wegen der besonderen Ernährungsweise schon seit langem Nährmedien eingesetzt, die vorwiegend auf pflanzlichen Grundlagen (z.B. Weizenkleie, Bermudagras etc.) basieren, die aber dennoch zumeist tierisches Eiweiß, z.B. Milchprodukte, Quark oder Hühnereier, als Zusatzstoffe enthielten ( z.B. Lit. P. Singh, RF. Moore, 1985, Handbook of insect rearing. Vol. 2, Elsevier, Seiten 125 ff).fly lay their eggs on species-specific preferred substrates. The most representatives of the group of the flesh flies (families Sarcophagidae and Calliphoridae) in nature always use meat (e.g. dead animals) or animal or human excreta as storage sites for their eggs, because the nutrients it contains apparently optimal for that Larval growth are (E. Martini 1946: Medical entomology, G. Fischer, Jena; W. Jacobs, M. Renner 1988, Biology and Ecology Insects: G. Fischer, Stuttgart; K. Dettner, W. Peters et al. 1999, textbook of entomology, G. Fischer, Stuttgart). The diet of Flesh flying is fundamentally different from that of saprophages Fly Musca domestica and related species (e.g., of the family Muscidae belong), where the larvae in nature also on putrid plant material to grow. Against this background, it is not surprising that the in many laboratories, breeding of the "flies" is actually exclusively under Use of meat or animal products (horse muscle, Pork liver etc.). These fly maggots thus produced and adult flies are used for a variety of purposes, e.g. For scientific studies, as food for reptiles, as baits for fish while fishing, etc. In the breeding of representatives of the saprophagen species (e.g., Musca domestica) were on the other hand because of the particular diet for a long time nutrient media mainly based on vegetable principles (e.g., wheat bran, Bermudagras etc.) are based, but still mostly animal Protein, e.g. Dairy products, quark or chicken eggs, as additives (e.g., Ref P. Singh, RF Moore, 1985, Handbook of insect rearing. Vol. 2, Elsevier, pages 125 ff).
Für die allermeisten Anwendungen der im Labor gezüchteten Fliegen bzw. ihrer Larven ist es ohne Bedeutung, auf welcher Nährstoff-Basis sie gezüchtet werden. Dies sieht allerdings anders aus bei solchen Fliegen, die dafür gezüchtet werden, um – wie z.B. Lucilia sericata – als Larven auf menschlichen Wunden zum Einsatz zu kommen. Mit dieser sogenannten Madentherapie können schlecht heilende Wunden erfolgreich behandelt werden. Die Fliegenmaden bewirken mit ihren Sekreten, dass nekrotisches Gewebe aufgelöst wird (Wunddebridement) und dass anschließend eine Ausheilung der Wunde erfolgt. Ein ungelöstes Problem bei der Madentherapie besteht darin, dass die eingesetzten Fliegenmaden aus ihrer tierischen Nahrung Viren, Bakterien oder Pilze aufnehmen und dann in die menschlichen Wunden übertragen können. Hierfür kommen eine große Zahl von unterschiedlichen Erregern als Kandidaten in Frage (W. Köhler et al. 2001; Medizinische Mikrobiologie, Urban und Fischer, München). Diese Liste potentieller Erregerübertragungen wurde noch um eine weitere Gruppe – die Prionen – erweitert, als von unserer Gruppe gezeigt werden konnte (Post et al. 1999: Fly larvae and pupae as vectors for scrapie, Lancet 354 pp., 1969–1970), dass Prionen im Inneren von Fliegenmaden und – puppen überleben können und dann – bei oraler Aufnahme – zu patenten Infektionen bei neuen Wirten führen. Aufgrund der möglichen Erregerübertragungen hat die Zucht von Fliegenlarven auf Fleischbasis den gravierendebn Nachteil, dass ein Einsatz dieser Maden am bzw. auf der Haut des Menschen mit unkalkulierbaren Risiken verbunden ist.For the vast majority Applications of laboratory-bred Flies or their larvae it is irrelevant, on which nutrient basis she bred become. However, this looks different for such flies that to be bred for um - how e.g. Lucilia sericata - as To use larvae on human wounds. With this so-called Mad therapy can badly healing wounds are treated successfully. The fly maggots With their secretions they cause necrotic tissue to disintegrate (Wunddebridement) and that then follows a healing of the wound. An unresolved one Problem with the Madentherapie is that the used Fly maggots from their animal food viruses, bacteria or Pick up mushrooms and then transfer them to human wounds can. Therefor come a big one Number of different pathogens as candidates in question (W. Charcoal burner et al. 2001; Medical Microbiology, Urban and Fischer, Munich). This list of potential pathogen transmissions was extended by another group - the prions - could be shown by our group (Post et al 1999: Fly larvae and pupae as vectors for scrapie, Lancet 354 pp., 1969-1970), that prions can survive inside fly maggots and pupae and then - in oral Recording - too patents cause infections in new hosts. Because of the possible pathogen transmissions the breeding of meat-based fly larvae has the serious disadvantage that use of these maggots on or on the skin of man associated with incalculable risks.
Eine Lösung des Problems durch die vorliegende Erfindung besteht darin, dass Fliegenmaden ohne Kontamination mit Krankheitserregern aufgezogen werden können. Es wurde in überraschender Weise gefunden, dass sich die Larven von Arten der fleischfressenden Gattungen Sarcophaga, Lucilia, Calliphora und Phormia auch auf ausschließlich pflanzlichen Substraten züchten lassen. Es wurde gefunden, dass die auf Pfalzenmaterialien gezüchteten Fliegen ähnliche Reproduktions- und Wachstumsraten aufweisen und sich auch zu Puppen und schließlich zu adulten Fliegen entwickelten. Von Vorteil erwies sich auch, dass diese pflanzlichen Medien vorher sterilisiert werden konnten und somit die gezüchteten Fliegenlarven, Puppen und Adulten frei sind Kontaminationen mit Mikroorganismen als auch von tier- oder humanpathogenen Erregern. Damit können diese Fliegenstadien (Larven, Puppen) bzw. aus diesen gewonnene Extrakte problemlos und ohne Gefahr der Übertragung von Erregern auf menschlichen Wunden zur Wundheilung eingesetzt werden.A solution The problem with the present invention is that Fly maggots raised without contamination with pathogens can be. It was in a surprising way found that the larvae of species of carnivorous genera Sarcophaga, Lucilia, Calliphora and Phormia are also exclusively herbal Grow substrates to let. It was found that those bred on plywood materials Flies similar Have reproduction and growth rates and also become pupae and finally developed into adult flies. Another advantage was that These herbal media could be previously sterilized and thus the bred Fly larvae, pupae and adults are free contaminations with Microorganisms as well as animal or human pathogens. With that you can These fly stages (larvae, pupae) or from these won Extracts easily and without risk of transmission of agents to human Wounds are used for wound healing.
Beispiel zur Herstellung erfindungsgemäßer Fliegenaufzuchtmedien Hier können folgende aus Pflanzen gewonnene Nahrungstoffe in Kombination zum Einsatz kommen:
- 1. Sojabohnenquark (Tofu) = jap. to = Bohne, Fu = Gerinnung, Gärung; wird durch Zusatz von Gerinnungsstoffen (z.B. Ca SO4) aus „Sojamilch" = Saft zerdrückter Bohnen hergestellt.
- 2. Sojamehl
- 3. Sojakonzentrat
- 4. Sojaisolat
- 5. Weizenkleie
- 6. Weizengries
- 7. Maisgries
- 8. Zuckerrübensirup
- 1. Soybean curd (tofu) = jap. To = bean, Fu = coagulation, fermentation; is made by adding coagulants (eg C a SO 4 ) from "soymilk" = juice of crushed beans.
- 2. Soymeal
- 3. Soya concentrate
- 4. soy isolate
- 5. Wheat bran
- 6. Wheat semolina
- 7. Maize grits
- 8. Sugar beet syrup
Tofu bzw. tofuhaltige Substrate werden ohne weitere Vorbehandlung eingesetzt und mit den anderen Produkten wie Pflanzenmehle, Weizenkleie, Gries und Sirup verrührt. Die Ansätze mit Sojamehl, -konzentrat bzw. -isolat werden aber zuvor für 3–5 Minuten in kochendem Wasser erhitzt und erst nach Abkühlen als Nahrung und Eiablagesubstrat verwendet. Da der Proteingehalt der eingesetzten Pflanzenstoffe zwischen 16–90% variiert, kann durch Einsatz verschiedener Mengen sowie durch Kombination mehrerer Pflanzenstoffe der Proteingehalt des Substrats auf die Anforderungen der jeweiligen Art eingestellt werden.Tofu or tofuhaltige substrates are used without further pretreatment and with the ande other products such as plant meal, wheat bran, semolina and syrup. The soybean meal, concentrate or isolate, however, are first heated in boiling water for 3-5 minutes and then used as food and egg laying substrate after cooling. Since the protein content of the phytochemicals used varies between 16-90%, the protein content of the substrate can be adjusted to the requirements of the respective species by using different amounts and by combining several phytochemicals.
Anwendungsbeispiele:Application examples:
Beispiel 1: Sarcophaga carnariaExample 1: Sarcophaga carnaria
Für die Zucht von S. carnaria ist folgende Nährstoff-Mischung geeignet:
- 1. 100 g Tofu
- 2. 6,5 g Weizenkleie
- 3. 13,5 g Zuckerrübensirup
- 1. 100 g of tofu
- 2. 6.5 g of wheat bran
- 3. 13.5 g sugar beet syrup
Fleischfliegen können auch auf Pflanzensubstraten folgender Zusammensetzungen ohne Fleisch gezüchtet werden:fleshflies can also be grown on plant substrates of the following compositions without meat:
Beispiel 2: Lucilia sericataExample 2: Lucilia sericata
- 1. 50 g Sojakonzentrat1. 50 g of soy concentrate
- 2. 3 g Lupinenmehl2. 3 g of lupine flour
- 3. 6,5 g Maisgries3. 6.5 g of maize grits
- 4. 10,0 g Zuckerrübensirup4. 10.0 g sugar beet syrup
Beispiel 3: Calliphora erythrocephalaExample 3: Calliphora erythrocephala
- 1. 150 g Tofu1. 150 g of tofu
- 2. 30 g Sojakonzentrat2. 30 g soy concentrate
- 3. 20 g Zuckerrübensirup3. 20 g sugar beet syrup
- 4. 10 g Weizenkleie4. 10 g of wheat bran
Beispiel 4: Phormia reginaExample 4: Phormia regina
- 1. 150 g Tofu1. 150 g of tofu
- 2. 50 g Sojamehl2. 50g of soy flour
- 3. 15 g Weizenkleie3. 15 g of wheat bran
- 4. 10 Lupinenmehl4. 10 lupine flour
- 5. 30 ml Zuckerrübensirup5. 30 ml sugar beet syrup
Die genannten Pflanzensubstrate sind nur als Beispiele gedacht. Die Rezepturen dürfen jedoch nicht als einschränkend aufgefaßt werden, weil Bestandteile von Pflanzen vieler Arten in erfindungsgemäßer Weise verwendet werden können. Die Nährsubstrate können in vielfältigen Kombinationsmöglichkeiten durch Zusatzstoffe ergänzt werden, beispielsweise können synthetische oder natürliche Vitamine, Mineralstoffe, Spurenelemente, Lipide, Kohlenhydrate oder mikrobizide Wirkstoffe zugesetzt werden.The said plant substrates are intended only as examples. The Recipes allowed but not as limiting conceived are because constituents of plants of many kinds in accordance with the invention can be used. The nutrient substrates can in diverse possible combinations supplemented with additives can, for example, be synthetic or natural Vitamins, minerals, trace elements, lipids, carbohydrates or microbicidal agents are added.
Die auf den erfindungsgemäßen Nährsubstraten gezüchteten Fliegen-Entwicklungsstadien können für die Wundtherapie lebend in den Handel gebracht werden oder nach Extraktion von wundheilenden Inhaltsstoffen zu medizinischen Präparaten verarbeitet werden.The on the nutrient substrates according to the invention bred Flying stages can for the Wound Therapy can be marketed live or after extraction from wound healing ingredients to medical preparations are processed.
Claims (11)
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| DE2003128102 DE10328102A1 (en) | 2003-06-20 | 2003-06-20 | Growing flesh flies on plant-based substrates, used to produce larvae and pupae for promotion of wound healing, eliminates risk of transferring pathogens associated with use of animal-based nutrient media |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| DE2003128102 DE10328102A1 (en) | 2003-06-20 | 2003-06-20 | Growing flesh flies on plant-based substrates, used to produce larvae and pupae for promotion of wound healing, eliminates risk of transferring pathogens associated with use of animal-based nutrient media |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| DE10328102A1 true DE10328102A1 (en) | 2005-01-20 |
Family
ID=33520805
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| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| DE2003128102 Ceased DE10328102A1 (en) | 2003-06-20 | 2003-06-20 | Growing flesh flies on plant-based substrates, used to produce larvae and pupae for promotion of wound healing, eliminates risk of transferring pathogens associated with use of animal-based nutrient media |
Country Status (1)
| Country | Link |
|---|---|
| DE (1) | DE10328102A1 (en) |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| DE102007034119A1 (en) | 2007-07-21 | 2009-01-22 | Agiltera Gmbh & Co. Kg | Use of blood, blood plasma or blood serum, and apparatus and method for growing fly larvae |
| FR2930116A1 (en) * | 2008-04-16 | 2009-10-23 | Inst Rech Pour Le Dev I R D Et | PRODUCTION AND USE OF INSECT LIVING MINI-LARVAES FOR AQUARIUM FISH FEEDING AND / OR ALEVINS OF ANIMAL FISH |
Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4840800A (en) * | 1987-11-16 | 1989-06-20 | Harris Robert L | Soybean fiber in insect rearing media |
-
2003
- 2003-06-20 DE DE2003128102 patent/DE10328102A1/en not_active Ceased
Patent Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4840800A (en) * | 1987-11-16 | 1989-06-20 | Harris Robert L | Soybean fiber in insect rearing media |
Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| DE102007034119A1 (en) | 2007-07-21 | 2009-01-22 | Agiltera Gmbh & Co. Kg | Use of blood, blood plasma or blood serum, and apparatus and method for growing fly larvae |
| EP2018805A2 (en) | 2007-07-21 | 2009-01-28 | AGILTERA GmbH & Co. KG | Use of blood, blood plasma or blood serum, and device and method for breeding fly larvae |
| FR2930116A1 (en) * | 2008-04-16 | 2009-10-23 | Inst Rech Pour Le Dev I R D Et | PRODUCTION AND USE OF INSECT LIVING MINI-LARVAES FOR AQUARIUM FISH FEEDING AND / OR ALEVINS OF ANIMAL FISH |
| WO2009136057A2 (en) | 2008-04-16 | 2009-11-12 | Institut De Recherche Pour Le Developpement (I.R.D.) | Production of live insect “mini-larvae” and use thereof for feeding aquarium fish, alevins of farm fish and pets |
| WO2009136057A3 (en) * | 2008-04-16 | 2009-12-30 | Institut De Recherche Pour Le Developpement (I.R.D.) | Production of live insect “mini-larvae” and use thereof for feeding aquarium fish, alevins of farm fish and pets |
| AP4056A (en) * | 2008-04-16 | 2017-03-08 | Inst Rech Pour Le Développement Ird | Production of live insect mini-larvae and use thereof for feeding aquarium fish, alevins of farm fish and pets |
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