DE10316598A1 - Centrifuge for the recovery of biological fluids e.g. thrombocytes from whole blood, has slits at the rotor periphery to take suspension hooks for the bodies containing the sample tubes - Google Patents

Abstract

The centrifuge assembly, for the recovery of biological fluids, has a rotor (1) rotating around a center axis (2). Suspensions (4) are attached to the periphery of the rotor which, during rotation, swing radially outwards through the centrifugal forces to carry centrifugal bodies (10a) containing tubes of biological fluids. The suspensions are Z-shaped hooks, to be inserted into slits (3) at the rotor.

Description

The The invention relates generally to a method for obtaining biological liquids as a fraction of one or more connected in series Centrifugation operations. If in the following description only platelet collection described from whole blood, this is not restrictive to understand.

It is known to heal bone defects and the like activities the addition of the body's own Platelets are very helpful and accelerate the reconstruction the bone substance leads, be it in jaw surgery to anchor dentures, in orthopedic surgery or in accident surgery to remove accident damage.

Various Procedures for the same purpose are not only very cumbersome but also also relatively non-sterile because it has "open systems" and the disadvantages work from high costs with imprecise reproducible quality.

The The invention is therefore based on the object of a method and a device in particular for the production of platelets To further develop blood and blood components of the type mentioned at the beginning, that the device can be used several times and in particular with conventional Blood bags are working. It is intended for a device that can be used for this purpose the manufacturing costs can be significantly reduced.

to solution the task is the invention through the technical teaching of claim 1. It is essential that with one and the same blood bag two different spins are carried out. In the first spin cycle, the straight, elongated blood bag centrifuged while in the following Centrifugation of the kinked blood bag.

A for the Usable methods according to the invention Centrifugal device consists essentially of device parts, which once centrifuged the elongated, straight and to the second is centrifugation of the same, bent blood bag enable.

The Invention is not based on the production of endogenous platelets (in particular for the Surgery). With the method according to the invention, platelets in others can Areas of application of medicine are obtained from blood or blood components.

The Invention beats thus an inexpensive Process before, which is also suitable, for example, for dentistry is. Here must only small amounts of platelets can be obtained from whole blood.

to Improvement of the known, relatively cumbersome and very expensive platelet production - predominantly in an open system - becomes the invention a very simple, inexpensive, practical and closed system proposed as a method and device, not only for extraction platelet is suitable, but also for the production of stem cells from umbilical cord blood may be applicable.

to Platelet recovery will give the patient a volume of approximately 20 ml of blood drawn into a blood bag (for example with a dimension of approx. 150 mm long and approx. 40 mm wide), in which an anticoagulant template, An anti-coagulant CPD is included in its amount corresponds to the blood volume to be drawn.

This not bulging, but loosely filled blood bags is now the length after put into a two-part, box-like spinner and on its upper half locked with the removable lid, creating the stable position during the Centrifugation is secured. The blood bag takes a tablet-like one Shape and is centrifuged weakly (at about 300 g), whereby itself radially outside the accumulation of erythrocytes and inside form the platelet-rich plasma.

At the This state ends at the end of this first weak centrifugation made visible by removing the lid and the bag on the Dividing line kinked and squeezed and optionally immediately - or after a certain rest period - sharper at centrifuged approx. 1000 g, with the lid in the second position has been moved and the kinked half of the bag is secure and also tabular again encloses.

On the back the bag half the erythrocyte concentrate that does not form in a few minutes more interested, and on the front, bent half on The end of the bag of the bag contains the platelet concentrate, which is now after removing the cover the hose can be easily removed.

The plasma above is either squeezed off with a simple clamp or partly in a desired ratio the platelets are mixed to obtain a preferred and appropriate dilution.

Consequently the whole process takes place until removal in a single closed system, namely in the little blood bag used.

to Obtaining larger quantities become bigger blood bags in correspondingly larger devices centrifuged and processed, or the amount is divided into several smaller ones Split bags.

The The subject of the present invention does not arise only from the subject of the individual claims, but also from the combination of the individual claims among themselves.

All in the documentation, including the information and features disclosed in the summary, in particular the spatial training shown in the drawings claimed as essential to the invention, insofar as they are used individually or in Combination versus the state of the art are new.

It demonstrate:

1 : a centrifuge according to the invention, each with two centrifugal devices in different process steps;

2 : the centrifuge rotor disc;

3 : the elbow for hanging the spinner into the rotor disc;

3a : A rear view of the spinner

4 : schematized a blood bag in plan view;

5 : Top view of the blood bag after 4 in the bent state;

6 : the spinner in perspective in side view;

7 : the lid to the spinner;

8th : equipping the spinner with the blood bag before closing the lid;

9 : the spinner after 8th in the finished state to carry out the first centrifugation;

10 : the retrofitting of the centrifugal device during the transition from the first to the second centrifugation;

11 : the converted state of the spinner with the components in an exploded view;

12 : the converted centrifugal device for carrying out the second centrifugation;

13 : one versus the 6 to 12 modified embodiment of a centrifugal device before or after carrying out the first centrifugation;

14 : the spinner after 13 to carry out the second centrifugation.

The 1 shows a disc rotor 1 , which preferably consists of a rustproof metal material. It is in the area of its axis of rotation 2 rotatably supported and driven in rotation in a manner not shown. It is preferably used in a boiler, not shown.

A swing bucket on a disc rotor of a general kind is, for example, in your own EP 0 612 565 B1 described. Reference is made to the disclosure there.

This is intended to be encompassed by the disclosure of the present invention. However, the invention is not based on the arrangement of the centrifugal devices to be explained later 10 . 40 in a disc rotor 1 limited.

The inventive concept also extends (use of centrifugal devices 10 . 40 with blood bags inside 7 ) also on all known angle head or inclined bed centrifuges.

For the sake of simpler explanation, only the embodiment of a swing-out suspension with the disk rotor shown is shown with the aid of the drawings 1 shown.

In the slots 3 of the disc rotor 1 are contra-angles 4 to 3 hooked. It essentially consists of an internal bend 5 which through the slot 3 of the disc rotor 1 passes through, an adjoining flat support part 6a , which is located on the rear wall of the spinner 10 . 40 creates and a second support part bent approximately perpendicular to it 6b , which is the base support for the spinner to be placed on it 10 . 40 forms.

According to 1 instructs each spinner 10 mutually directed and parallel guide ribs 14 which form an insertion groove between them.

This insertion groove is on the side lugs 6c of the contra-angle 4 postponed so that the spinners 10 . 40 on the contra-angles 4 are pushed and from the bottom-side support part 6b be worn.

For better clarity are in 1 two different conversion states of the respective spinner 10 shown.

With the reference symbol 10 Two opposing centrifugal devices are shown before or after the end of the first centrifugation process, while with the reference symbol 10a the same spinners 10 , but are shown in the modified state, before or after the second centrifugation process.

In practice, however, all point to the disc rotor 1 attached slingshots 10 . 10a always the same retrofit status.

The 4 and 5 now explain schematically how the centrifugation of the blood bag 7 takes place in the two different centrifugation processes connected in series.

It becomes a relatively small, narrow, approximately rectangular blood bag 7 used, the interior of which is half-filled with whole blood. The filling takes place via the hose 9 , the liquid-conducting approach 8th into the interior of the blood bag 7 introduces.

After the first centrifugation process, settle in the bottom area (the tube 9 opposite) the heavier erythrocytes 45 starting like this in 4 is indicated.

The platelet-rich PRP forms in connection with the plasma above this fraction 46 ,

To carry out the second centrifugation process, the blood bag is now 4 approximately in its middle area around a crease edge 34 in the direction of the arrow 48 bent over so that it reshaped 5 receives.

It is important that the crease edge 34 through a sliding clamp 37 or is sealed by another squeezing agent in such a way that there is no liquid-conducting transition between the two blood bag parts which are now forming 42 . 44 arises.

Now the blood bag 7 to 5 centrifuged again, then accumulate in the right chamber (lower blood bag part 44 ) nevertheless only erythrocytes and plasma, while in the other chamber (upper blood bag part 42 ) the desired platelets from the PRP fraction 47 down near the hose attachment 8th of the hose 9 drop.

In order to is the one you want Separation of platelets from whole blood into two different, centrifugation processes in succession achieved.

The 4 is just for the sake of clarity (with a straight blood bag 7 ) shown that the platelets 47 about near the approach 8th on the hose 9 deposit. In reality, however, the blood bag has a bent shape 5 ,

In 6 is the first embodiment of a spinner 10 shown, which preferably consists of a plastic material.

The spinner 10 has a lower support plate 11 on, which extends approximately over the entire length of the device. Above, parallel and at a distance from this is an approximately half as long upper support plate 15 arranged above.

Between the lower, longer support plate 11 and the upper, shorter support plate 15 thus becomes a lower compartment 36 for inserting the foot end of the blood bag (namely the blood bag part 44 ) created.

At the back of the support plate 11 are opposing guide grooves 14 arranged in the representation in 3a are photographed, in which the lateral approaches 6c of the contra-angle 4 intervention.

The lower support plate 11 is through side guide edges 12 limited, on the outer surfaces associated guide ribs 23 are arranged, which the sliding guide and the holder for a sliding cover to be pushed thereon 21 (please refer 7 ) form.

Likewise, the upper support plate has 15 lateral guide edges arranged parallel to each other and delimiting the plate 16 guide ribs on the outside 24 are arranged, which also as a sliding guide and holder for the in 7 shown sliding cover 21 are trained.

This forms the upper support plate 15 an upper compartment 35 , in which in a later procedure the broken blood bag part 42 of the blood bag 7 folded and held by the sliding cover. The bottom part of the spinner 10 is through a floor area 17 formed, the surface of which is approximately perpendicular to the plane of the support plates 11 . 15 is. This floor area 17 is by an approximately arch-shaped circumferential support edge 18 limited, on the side slots 19 are incorporated.

So there are two different and independently working sliding guides and brackets for the in 7 illustrated lid. Only one sliding guide or bracket is required.

On the one hand, it can be provided that on the inside of the in 7 shown sliding cover 21 on the side approaches 25 in the longitudinal direction and parallel to each other arranged guide ribs are arranged, each in the direction of the cover side a guide groove 26 form.

The previously mentioned guide ribs engage in this guide groove 23 and optionally - depending on the mounting arrangement of the sliding cover 21 - also the guide ribs 24 according to 6 on.

The invention is of course not on the holder of the sliding cover 21 limited by means of the described sliding guides or slot holders. It can also be provided that the lid can be pivoted or folded (for example via film hinges) on the spinner 10 is arranged.

In the area of the cover plate 22 of the sliding cover 21 is a recess, by the way 51 for the passage of the hose 9 of the blood bag 7 arranged.

On the axially opposite side of the cover plate, this is in the form of a narrower attachment 28 Extends upwards and forms a guide collar in connection with arched, lateral edges 29 for guiding the hose 9 out.

The 8th now shows the preparation of the spinner 10 to carry out the first centrifugation process.

This is the blood bag 7 as a straight, elongated table with its lower end in the lower compartment 36 the spinner 10 inserted and then the sliding cover 21 in the direction of the arrow 31 on the support plate 11 pushed so that the blood bag 7 is protected against twisting or warping. Here is the lower edge 52 of the sliding cover 21 on the crease edge 34 on.

This is the sliding cover 21 secure and secure with the centrifuge 10 connected. The 9 shows the now completed assembly position of the sliding cover.

At the end of the first centrifugation process, the sliding lid 21 in the direction of the arrow 32 removed upwards and the blood bag 7 with its upper end in the direction of the arrow 33 over the crease edge 34 the spinner 10 folded down ( 10 ).

This will separate the two blood bag parts 42 and 44 defined, according to 5 the blood bag part 42 in the upper compartment 35 comes to rest while the other blood bag part 44 in the lower compartment 36 the spinner 10 remains. The kinked shape of the blood bag 7 is in 11 shown.

To make an absolutely tight seal between the two parts of the blood bag 42 . 44 in the area of the fold edge 34 to ensure, according to the invention 11 a sliding clamp 37 used, which is placed on the previously described sliding guide. Here, the sliding clamp 37 mutually opposite and parallel guide grooves 39 on that with the associated guide ribs 23 on the side wall of the support plate 11 interact.

The sliding clamp 37 has at its lower end opposite and approximately V-shaped narrowing plate parts with a tapered clamping opening between them 38 form.

The sliding clamp is thus in the direction of the arrow 31 on the support plate 11 pushed open and the clamp opening 38 engages positively and positively over the kink edge 34 and squeezes the blood bag 7 in the area of the fold edge 34 absolutely close together. It is a self-locking arrangement, so the sliding clamp 37 can no longer solve itself.

After installing the slide clamp 37 becomes the sliding cover 21 now according to 12 on the upper support plate 15 postponed.

The exemplary embodiment shown shows that the guide grooves 53 of the sliding cover 21 with the assigned guide ribs 24 interact.

The sliding cover is thus on the guide ribs 24 pushed on and secures the kinked blood bag, especially the upper part of the blood bag 42 against bulging or deformation. The recess 51 of the sliding cover 21 overlaps the kinked and closed hose 9 ,

The centrifuge rebuilt in this way 10a according to 12 (see also 1 ) is now a longer centrifugation with a higher acceleration force of z. B. subjected to 1000 g.

It occurs according to 5 at the after 12 kinked blood bags separating the platelets 47 from the PRP, which is accordingly near the hose 9 accumulate.

After this centrifugation, the centrifuge is stopped and the entire spinner 10a is removed from the suspension of the centrifuge. The hose 9 is cut off, for example, and the platelets become in the direction of the arrow 41 taken.

This made platelets under absolutely sterile conditions and in the closed space of the blood bag 47 from the plasma 46 of whole blood.

The 13 and 14 show another spinner 40 , which is a modified version of the spinner 10 . 10a represents.

While the spinner 10 . 10a two support plates 11 . 15 with compartments trained on it 35 . 36 trained, this is the case with the embodiment 13 to 14 solved differently. It is shown there that the spinner 40 an upper compartment 35 forms, but at its upper free end journal 43 are arranged in which the lower compartment is pivotable 36 is stored.

In the first centrifugation step, the position of the centrifugal device is determined 40 to 13 , the elongated blood bag 7 in the pocket between the upper and lower compartments 35 . 36 is held.

A sliding cover, as described above, is also used around the one in the compartment 36 to hold lying blood bags and fix them against kinking.

At the transition from the first to the second centrifugation step, however, the entire compartment 36 around the trunnion 43 in the direction of the arrow 33 pivoted so that the position after 14 results.

The blood bag is over the crease 34 folded, which in turn turns the two blood bag parts 42 . 44 to 5 result. It can then be a sliding clamp 37 to the transition area in the area of the crease edge 34 be postponed.

In the second embodiment shown, it is no longer necessary to reposition a sliding cover 21 , because in the second conversion stage of the spinner 40a the support plate of the compartment 36 at the same time kink and position securing of the blood bag 7 forms.

The sliding cover is only in the conversion situation of the spinner 40 to 13 needed.

above the production of platelets from whole blood was described. hereupon however, the invention is not limited. It is generally claimed that under sterile conditions from one with a biological liquid filled pouch one by two centrifugation processes in series certain (sub) fraction is obtained.

in the The first centrifugation step is the biological fluid separated into at least two different fractions and the second The centrifugation step becomes one from the fraction obtained further fraction separated and won.

1

disc rotor

2

axis of rotation

3

slot

4

elbow

5

bend

6

supporting part a, b, approach c

7

blood bags

8th

approach

9

tube

10

Spinner ( 10a )

11

support plate (below)

12

leading edge

13

insertion tray

14

guide

15

support plate (above)

16

leading edge

17

floor area

18

supporting edge

19

slot

20

21

sliding cover

22

cover plate

23

guide rib

24

guide rib

25

approach

26

guide

27

28

approach

29

guide collar

30

the bottom part

31

arrow

32

arrow

33

arrow

34

bend edge

35

upper compartment

36

lower compartment

37

slide clamp

38

terminal opening

39

guide

40

spinner 40a

41

arrow

42

upper Blood bag part

43

pivot

44

lower Blood bag part

45

erythrocytes

46

plasma

47

platelets

48

direction of travel

49

50

51

recess

52

edge

53

guide

Claims (9)

Process for obtaining biological fluids, in particular platelets from blood and blood components by single or multi-stage centrifugation of a receptacle, the fractions obtained by centrifugation being separated from one another, characterized by the following process steps: 1.a blood bag filled with whole blood ( 7 ) is placed in the spinner ( 10 . 40 ) placed in a centrifuge and centrifuged weakly until erythrocytes form on one side and a platelet-rich plasma (PRP) forms on the other side, 2. the centrifuge is stopped and the spinner ( 10 . 40 ) opened, 3. the blood bag ( 7 ) is kinked approximately in the area of the phase boundary between the fractions and squeezed together in the area of the kink to form a seal, with two separate blood bag parts ( 42 . 44 ) 4. the kinked blood bag ( 7 ) is reattached in the centrifuge device and 5. centrifuged at least a second time until there is a blood bag part ( 42 ) a separation between plasma ( 46 ) and platelets ( 47 ) results, 6. When the centrifuge is stopped, the centrifugal device ( 10 . 40 ) removed from the rotor and from one blood bag part ( 42 ) of the kinked blood bag ( 7 ) the platelets over the tube ( 9 ). Process for obtaining biological liquids by single or multi-stage centrifugation of a receptacle, the fractions obtained by centrifugation being separated from one another, characterized by the following process steps: 1. a bag filled with the liquid is placed in the centrifugal device ( 10 . 40 ) a centrifuge is inserted and centrifuged weakly until two different fractions can be formed, 2. the centrifuge is stopped and the spinner ( 10 . 40 ) opened, 3. the bag is kinked approximately in the area of the phase boundary between the fractions and squeezed together in the area of the kink, forming two separate bag parts, 4. the kinked bag is in the spinner ( 10 . 40 ) attached again and 5. centrifuged at least a second time until there is a separation in the fraction contained in the one bag part, 6. with the centrifuge stopped, the centrifugal device ( 10 . 40 ) is removed from the rotor and one part of the bag is folded into a fraction over the tube ( 9 ). A method according to claim 1 or 2, characterized in that that the first centrifugation at about 300 g and the second Centrifugation takes place at about 1000 g. Method according to one of claims 1 to 3, characterized in that that the relationship from the volume of the blood bag to the volume contained therein chosen is that the blood bag is only filled loosely. Device for carrying out the method according to one of claims 1 to 4, characterized in that the centrifugal device ( 10 . 40 ) a first insertion pocket, which extends over about half the length of the blood bag (lower compartment 36 ) for inserting the blood bag ( 7 ), the axially upper limit of which has a kink edge ( 34 ) for the buckling of the blood bag ( 7 ) is formed. Device according to claim 5, characterized in that the blood bag ( 7 ) after folding over the fold edge ( 34 ) in another compartment ( 35 ) the spinner ( 10 ) can be folded in and fixed there. Device according to one of claims 5 or 6, characterized in that the layer fixation of the blood bag ( 7 ) with a sliding cover ( 21 ) he follows. Device according to one of claims 5 to 7, characterized in that the sliding cover ( 21 ) with sliding guides ( 16 . 23 . 24 ) on the spinner ( 10 ) is held removable. Device according to one of claims 5 to 7, characterized in that in the centrifugal device ( 40 ) the compartment ( 36 ) to hold the elongated blood bag ( 7 ) pivotable with respect to the crease edge ( 34 ) is trained.