DE10290599A5 - Fluorescent microparticles, useful as markers or tracers, comprise core of fluorescent material coated with metallic conductive material with further transparent protective layer - Google Patents
Fluorescent microparticles, useful as markers or tracers, comprise core of fluorescent material coated with metallic conductive material with further transparent protective layer Download PDFInfo
- Publication number
- DE10290599A5 DE10290599A5 DE10290599A DE10290599A DE10290599A5 DE 10290599 A5 DE10290599 A5 DE 10290599A5 DE 10290599 A DE10290599 A DE 10290599A DE 10290599 A DE10290599 A DE 10290599A DE 10290599 A5 DE10290599 A5 DE 10290599A5
- Authority
- DE
- Germany
- Prior art keywords
- fluorescent
- conductive material
- particles
- metallic conductive
- microparticles
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Withdrawn
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Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
- G01N33/54313—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals the carrier being characterised by its particulate form
- G01N33/54346—Nanoparticles
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- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Immunology (AREA)
- Chemical & Material Sciences (AREA)
- Urology & Nephrology (AREA)
- Hematology (AREA)
- Biomedical Technology (AREA)
- Molecular Biology (AREA)
- Medicinal Chemistry (AREA)
- Analytical Chemistry (AREA)
- Cell Biology (AREA)
- Nanotechnology (AREA)
- Food Science & Technology (AREA)
- Biotechnology (AREA)
- Physics & Mathematics (AREA)
- Microbiology (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- General Physics & Mathematics (AREA)
- Pathology (AREA)
- Luminescent Compositions (AREA)
- Investigating, Analyzing Materials By Fluorescence Or Luminescence (AREA)
- Investigating Or Analysing Materials By The Use Of Chemical Reactions (AREA)
Abstract
Fluorescent microparticles having an average particle diameter of 5-100 nm comprise a core of a fluorescent material coated with a metallic conductive material with a further transparent protective layer and an optional additional layer comprising functional groups or conjugate molecules that enable coupling to biomolecules or other analyte substances. Fluorescent microparticles (I) having an average particle diameter of 5-100 nm are characterized in that: (i) the particles comprise a core of a fluorescent material coated with a metallic conductive material (II) that stimulates the optical frequency and fluorescence such that the diameter of the core of the fluorescent material as well as the type and layer thickness of the metallic conductive material are formulated with respect to each other so that the maximum photostability and/or brightness is achieved by single- and/or multi-photon excitation; (ii) the particles are coated with a further transparent protective layer (III); and (iii) the particles optionally comprise functional groups or conjugate molecules (IV) that enable coupling to biomolecules or other analyte substances. An independent claim is included for the production of the microparticles (I) comprising: (A) preparation of the polymer particles that contain fluorescent organic dye or fluorescent semiconductor crystals having an average particle size of 5-100 nm; (B) coating the fluorescent particles from (A) with a metallic conductive material (II); and (C) coating the metalized particles with a protective transparent layer with an optional further coating (IV).
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| DE10290599A DE10290599A5 (en) | 2001-02-16 | 2002-02-12 | Fluorescent microparticles, useful as markers or tracers, comprise core of fluorescent material coated with metallic conductive material with further transparent protective layer |
Applications Claiming Priority (4)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| DE10108808A DE10108808A1 (en) | 2001-02-16 | 2001-02-16 | Fluorescent microparticles |
| DE10108808.6 | 2001-02-16 | ||
| DE10290599A DE10290599A5 (en) | 2001-02-16 | 2002-02-12 | Fluorescent microparticles, useful as markers or tracers, comprise core of fluorescent material coated with metallic conductive material with further transparent protective layer |
| PCT/DE2002/000556 WO2002066574A1 (en) | 2001-02-16 | 2002-02-12 | Fluorescent microparticles |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| DE10290599A5 true DE10290599A5 (en) | 2008-06-19 |
Family
ID=39400005
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| DE10290599A Withdrawn DE10290599A5 (en) | 2001-02-16 | 2002-02-12 | Fluorescent microparticles, useful as markers or tracers, comprise core of fluorescent material coated with metallic conductive material with further transparent protective layer |
Country Status (1)
| Country | Link |
|---|---|
| DE (1) | DE10290599A5 (en) |
-
2002
- 2002-02-12 DE DE10290599A patent/DE10290599A5/en not_active Withdrawn
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| 8110 | Request for examination paragraph 44 | ||
| R119 | Application deemed withdrawn, or ip right lapsed, due to non-payment of renewal fee |
Effective date: 20110901 |