DE102022127793B3 - Method and device for spectroscopy of a sample - Google Patents

Abstract

The present invention relates to a device (10) for spectroscopy of a sample (50), the device (10) comprising an excitation source (17) and at least one detector (18). The device (10) comprises at least one coupling element (11) with a first surface (12) and a second surface (15), the first surface (12) being flat, the first surface (12) having a first region (13 ) for coupling excitation light in the direction of the sample (50) and a second region (14) for coupling out light excited in the sample (50) in the direction of the detector (18), and wherein the first region (13) and the distinguish second area (14).

Description

The present invention relates to a device and a method for spectroscopy of a sample according to the independent claims.

State of the art

Devices for spectroscopy are basically known from the prior art, but these are very large and can therefore only be used to a limited extent. Furthermore, typically only light originating from a sample can be measured based on extinction or light-induced luminescence, such as fluorescence, so that not all relevant properties of the sample can be recorded at the same time.

DE 10 2020 007 928 A1 discloses a device designed as a microspectrometer with reflection optics.

US 2011/0 205 541 A1 relates to an optical measuring system with a hemispherical element that has different coatings.

DE 11 2016 001 775 T5 relates to a system with optical fibers that is designed for tissue examination.

DE 10 2017 208 463 A1 relates to an optical sensor for optically measuring a concentration of a component of a liquid or a measurement gas.

AT 512 238 A1 relates to a skin contact detection device that has a contact piece with a support surface.

CN 110 208 237 A relates to a multifunctional spectrometer in which a cone is used on which total reflection takes place.

US 10 234 331 B2 concerns a monolithic spectrometer.

Disclosure of the invention: task, solution, advantages

The present invention is based on the object of further developing a device for spectroscopy in such a way that as many relevant properties of a sample as possible can be recorded at the same time, while the device is also designed to be as small as possible in order to be used universally.

The aforementioned task is solved by a device for spectroscopy of a sample, the device comprising an excitation source and at least one detector. The device comprises at least one coupling element with a first flat surface and a second surface. At least a portion of the second surface can be designed to be in direct contact with the sample during a spectroscopic measurement.

The second surface can also be flat. It can run parallel to the first surface. In such a case, the entire second surface can be in direct contact with the sample.

Furthermore, the second surface can be curved. For example, only one point, preferably the vertex of the second surface, can be in direct contact with the sample.

Both the first and the second surfaces are preferably designed as a surface of the coupling element. The first surface and the second surface are formed in particular on opposite sides in a thickness direction of the coupling element. The thickness direction runs in particular perpendicular to the first surface.

The second surface can adjoin the first surface directly, so that the entire surface of the coupling element is formed by the first and second surfaces. If both the first and second surfaces are flat, the coupling element primarily has a third surface that connects the first surface and the second surface. This can preferably be a lateral surface.

The first area has a first area for coupling excitation light in the direction of the sample and a second area for coupling out light excited in the sample in the direction of the detector, the first area and the second area being different. In particular, the areas are completely different and therefore do not overlap.

Preferably, the first area and/or the second area comprises at least 20%, preferably at least 25%, most preferably at least 40%, of the first area. In particular, the first area is divided into two, so that both areas cover 50% of the area. The first surface is in particular circular.

The coupling element is preferably designed in the form of a hemisphere or half cylinder. The second surface is therefore curved, while the first surface is the flat surface of the hemisphere or half-cylinder and thus runs through its center. The vertex can the hemisphere or half cylinder comes into direct contact with the sample. In other words, the coupling element can be designed as a hemispherical lens or hemispherical lens. In particular, the second surface thus has the shape of a hemisphere or a half-cylinder, with the first surface running through a center of the hemisphere or half-cylinder.

In addition, the coupling element can have the shape of a capped ellipsoid, in other words a capped egg shape. In particular, the second surface has a parabolic shape in a section in the thickness direction of the coupling element.

The coupling element can also have the shape of a pyramid or a cone. Thus, the first surface can be the face of the pyramid or cone, while the second surface forms the shell including the tip. The tip of the pyramid or cone can come into direct contact with the sample. The pyramid can have a round or a rectangular, for example square, base.

Furthermore, the coupling element may have a shape composed of the shapes of a shape described above, for example a hemisphere, and the shape of a cylinder with the same diameter. The first surface can be designed as the end face of the cylinder, while the other end face is adjoined by the other shape, for example the hemisphere. The second surface can be the lateral surface of the cylinder together with the curved surface of the other shape. Even in such a case, the vertex of the hemisphere forms direct contact with the sample during a measurement.

All of the above-mentioned shapes can be designed as a truncation, for example as a truncated hemisphere, truncated cone or truncated pyramid. In other words, the shapes are capped or cut off so that the second surface is also flat and parallel to the first surface. For example, the coupling element can have the shape of a truncated hemisphere. The first surface forms the flat surface of the actual hemisphere, while the second surface can be the sectional surface. Furthermore, the coupling element can have the shape of an ellipsoid capped on both sides or the shape of a capped pyramid, i.e. a truncated pyramid.

Furthermore, the coupling element can have the shape of a polyhedron, preferably a convex polyhedron, the polyhedron approximating a shape described above. In this case, the first surface is once again flat, while the second surface consists of different flat areas, which overall reproduce a curved design.

The coupling element in particular has a spatial extent that is less than 20 μm, preferably less than 15 μm, most preferably less than 10 μm. Preferably the extension is at least 1 µm, preferably at least 3 µm, most preferably at least 5 µm. The expansion refers in particular to a direction transverse to a thickness direction of the coupling element. Since the first surface is particularly circular, the extent can primarily relate to its diameter. Furthermore, the values mentioned above can also apply to the expansion in the thickness direction of the coupling element.

The coupling element is in particular formed from a transparent material, preferably the material is dielectric. In particular, each coupling element is in one piece and formed from a single material, except for additional coatings if necessary. The material for the at least one coupling element is in particular glass or a polymer. Above all, quartz glass, fused silica, silicon dioxide (SiO2), sapphire, silicon, magnesium fluoride (MgF2), barium fluoride (BaF2), lithium fluoride, germanium (Ge), gallium arsenide (GaAs), zinc selenide (ZnSe), polycarbonate, acrylic ( PMMA), polyester or polydimethylsiloxane (PDMS) can be used as material. The above-mentioned materials can also be functionalized, in other words include antibodies or nanoparticles, or coated, for example by means of an anti-reflective or anti-scratch coating.

Above all, the first region of the first surface has a first coating, the first coating being designed as an optical filter. This can in particular be a bandpass or Fabry-Perot filter. The coating of the first region can in particular be designed to direct only a certain wavelength range of the excitation light towards the sample. A desired wavelength range is thus selected for excitation. The first area can also not be coated. When detecting excited light based on extinction, namely absorption and scattering, for example, a corresponding coating can be dispensed with.

The second region of the first surface can comprise a second coating, the second coating being designed as an optical filter is. The second coating can be designed as a longpass or Fabry-Perot filter.

In particular, the first and second coatings differ from each other. The coating of the second region can in particular be designed to direct only a specific wavelength range or only one wavelength of the light excited in the sample towards the detector. A desired wavelength range or just one wavelength is therefore selected for detection.

Thus, by means of the second coating, a distinction can be made between different modes, for example between extinction, namely absorption and scattering, and light-induced luminescence, for example fluorescence, of spectroscopy. For example, only excited light based on extinction or light-induced luminescence, for example fluorescence, can be transmitted.

Furthermore, an entire spectrum of excited light can be transmitted and thus detected using a Fabry-Perot filter. This results from the structural structure of the filter, which preferably comprises two mirrors that can be tilted towards one another. Depending on the tilt, different wavelength ranges result.

The second and/or the third surface of the at least one coupling element may comprise a third coating, wherein the third coating differs from the first and/or second coating. This is primarily designed to increase the excitation and/or detection efficiency through reflection on the coating. In particular, the third coating serves to guide the light deflection precisely in the direction of the detector and thus contributes to increasing efficiency.

It is preferably not designed as an optical filter. For example, the third coating may comprise gold and/or silver and/or aluminum.

The second surface, in other words the sample side, can be modified by means of the third coating, for example to enable the measurement of certain properties of the sample. For example, coatings can be used that enable the measurement of the pH value, the O2 and/or CO2 concentration and/or the pressure or ensure mechanical protection. PDMS (polydimethylsiloxane), for example, can be used as a coating. Microchannels can also be incorporated into the coating.

The excitation source is used to emit light to excite the sample and can in particular be designed as a laser or LED. Furthermore, the excitation source can be designed as a quantum dot layer that is excited with blue light. Each quantum dot preferably emits according to its physical radius (r) and scales the emission energy and thus the color of the photoluminescence, in other words the emission, with one over r-squared (1/r2). Particularly when using an LED as an excitation source, the first coating serves to selectively select the excitation wavelength. Furthermore, the first coating can be adapted depending on a different mode of the spectroscopy to be carried out. Depending on whether extinction or light-induced luminescence, for example fluorescence, is to be measured, a different first coating can be selected.

The geometry of the coupling element, preferably together with the coatings described above, ensures effective guidance of excitation light towards the sample and of light excited in the sample, such as based on light-induced luminescence, for example fluorescence, or extinction, to the detector.

According to the invention, the device has a plurality of coupling elements, the coupling elements being arranged according to the invention as an array, for example in rows and columns. In other words, the coupling elements form an array. Alternatively, the plurality of coupling elements can be arranged in a single row. All coupling elements are preferably identical in terms of their geometric shape.

An array is to be understood in particular as an arrangement in which the coupling elements are arranged on a common, flat or curved plane. The array, which is formed by the coupling elements, is therefore to be understood as a three-dimensional body, with two dimensions being designed to be significantly larger than the dimension in the thickness direction of the array. The array can be understood as a matrix. There can be as many rows as there are columns. The coupling elements can be applied to a common substrate, for example a flexible substrate.

In particular, the device has a first and an adjacent second coupling element, each of which comprises a first coating on the first region of the first surface and/or a second coating on the second region of the first surface, wherein the first coating and/or the second Coating of the first and of the second coupling element. Advantageously, at least two adjacent coupling elements therefore have different first and/or second coatings. Furthermore, at least two adjacent coupling elements can have different third coatings. In particular, all adjacent coupling elements have first coatings and/or second coatings and/or third coatings that are different from one another. Alternatively, all coupling elements can have the same third coating.

Each coupling element can be assigned its own detector. In particular, a detector can rest on every second area of the first surface. If each individual coupling element is assigned its own detector, this is in particular a photodiode.

Furthermore, a common detector can be assigned to all coupling elements of the device. In other words, the detector can cover the entire array formed. Each coupling element can, for example, be assigned a pixel of the common detector. Furthermore, several pixels can also be assigned to each coupling element. The common detector can in particular be a CCD detector. CCD stands for Charge Coupled Device. The CCD detector consists of a matrix of light-sensitive photodiodes, whereby a photodiode can be assigned to a coupling element.

When a coupling element is designed as a half cylinder, its length can extend over at least five times, preferably at least ten times, the diameter. Several samples can be measured simultaneously by means of a coupling element, which is designed, for example, as a half cylinder, by assigning different samples to different sections of the second surface. A coupling element can therefore be assigned several detectors, for example a detector for a section along the length of the coupling element.

Preferably, the device can have a plurality of semi-cylindrical coupling elements, which can be arranged parallel to one another.

The device preferably has only one excitation source. Therefore, not every coupling element is assigned its own excitation element. By arranging the coupling elements in a common arrangement, preferably in an array, they can advantageously be illuminated over a large area, so that no complex adjustment of individual excitation sources is necessary. A common excitation source can therefore be used that illuminates all coupling elements.

The desired excitation wavelength is selected in particular by the first coatings of the first regions of the first surfaces of the coupling elements. In particular, the device has at least three, preferably at least five, most preferably at least seven, coupling elements with a different first coating and thus differently selected excitation light.

Because different coupling elements advantageously have different first coatings, a large wavelength range can be achieved with regard to the excitation. For example, at least one coupling element may have a first coating that selects UV light for excitation, while another coupling element may have a first coating that selects infrared for excitation. In particular, the first coatings are selected such that the plurality of coupling elements cover an excitation wavelength range from UV to infrared.

In particular, the device has at least three, preferably at least five, most preferably at least seven coupling elements with a different second coating and thus differently selected light for detection. Different signals, such as those based on extinction or light-induced luminescence, can therefore be detected simultaneously using the large number of coupling elements. For example, at least one coupling element may have a second coating that selects UV light for detection, while another coupling element may have a second coating that selects infrared for excitation. In particular, the second coatings are selected such that the plurality of coupling elements cover a detection wavelength range from UV to infrared.

The array preferably comprises at least 9, preferably at least 16, coupling elements. The number of coupling elements determines the energy resolution of the spectroscopy method. In particular, the coupling elements are arranged in rows and columns, the device preferably comprising at least three, further preferably at least five, most preferably at least seven, rows and/or columns of coupling elements.

For example, the first coating can change from line to line the second coating changes from column to column. In detail, the rows may extend in a first direction while the columns extend in a second direction. The first coatings can differ between coupling elements adjacent in the first direction, while the first coatings of the coupling elements in the same row are identical. Furthermore, the second coatings may differ between coupling elements adjacent in the second direction, while the second coatings of the coupling elements in a same column are identical. The first coatings can thus be constant in a row while varying from column to column, while the second coatings do not change within the same column but vary from row to row.

By appropriately providing first coatings and second coatings, a large number of combinations of first coatings to second coatings are given, which enables a very large possibility of combining excitation wavelengths to detection wavelengths. The optical excitation and detection variability of the device is thus maximized.

Furthermore, for example, different rows and/or columns can be used for the simultaneous detection of different signals, for example for the detection of excited light based on light-induced luminescence and excited light based on extinction, namely absorption and scattering, by appropriate selection of the detection wavelength using the second coating . Additional physicochemical parameters, for example pressure or temperature, can also be measured using appropriate third coatings, which can vary from row to row or column to column or from coupling element to coupling element. Furthermore, for example, a row and/or column of the array can be used for internal calibration. This allows a known sample to be scanned using the row or column for calibration.

Due to the different first and/or second coatings, specific coupling elements can be specifically selected for a measurement. A conscious selection of coupling elements for carrying out the measurement can therefore be made. For example, if only a few wavelengths are required for a measurement process, just a few coupling elements can be sufficient to determine the desired measurement data.

The arrangement of the coupling elements in an array enables the greatest possible flexibility with regard to spectroscopy. By varying the excitation wavelength and varying the detection wavelength through different first and second coatings, for example, the entire spectral range from UV to infrared can be covered, and this with simple and inexpensive coupling elements.

The sample is in particular the surface of an object. In particular, surfaces can be measured using the present device in order to derive interface interactions, for example biocompatibility, wettability, paintability, or surface loadings, for example contamination or moisture.

In particular, the device does not include any optically dispersive elements, such as gratings or prisms, which are used primarily to select excitation light and/or to select light excited in the sample, since the spectral selection for excitation by means of the first coating and the spectral selection of the Light coming from the sample is carried out using the second coating.

Furthermore, the device does not include any mechanically movable components, for example to deflect excitation light in different directions and/or to receive excited light from different directions in the sample. As a result, the device is particularly robust.

Furthermore, by dispensing with moving parts and more dispersed elements, each coupling element and thus the entire device can be made significantly smaller. This is further supported by the fact that a single pixel can be sufficient for detection, so that a single coupling element can be made particularly small. Overall, the array of coupling elements can have a dimension transverse to the thickness direction, in other words an edge length, of less than 7 mm, preferably less than 5 mm, particularly preferably less than 3 mm, and still have a high density of coupling elements. Preferably both dimensions are formed transversely to the thickness direction according to the above values.

An array of coupling elements is easy to produce. For example, an array of coupling elements can be printed by 3D nanoimprint. Miniaturized training also opens up universal application possibilities. Furthermore, the dispersive optical elements of classic spectrometers are often not only a limiting factor in terms of size, but also in terms of price. Because the present device does not require corresponding dispersive elements, it is not only small but also particularly cost-effective.

Overall, it is a multispectral device, with the multispectral character preferably relating to both the excitation wavelength and the detection wavelength. Furthermore, the device is designed to be multimodal, since different modes of spectroscopy can be used selectively, preferably even simultaneously. In other words, the device is a multimodal optical sensor array. The compactness and degree of miniaturization, together with the variability of the optical spectroscopy methods, open up a wide range of possible applications, for example the measurement of food surfaces.

In a further aspect, the invention relates to a method for spectroscopy of a sample, which comprises emitting excitation light by means of an excitation source, wherein the excitation light is directed onto the sample through a respective first region of a first flat surface of a coupling element.

Light excited in the sample is directed towards the detector through a second region of the first surface, the first region and the second region of the first surface being different. The coupling elements are arranged as an array. In particular, the method is carried out using a device described above.

The method may include a prior selection of a number of coupling elements. This selection can be made depending on the light to be excited from the sample to be detected. For example, the selection can be made based on the at least one desired mode of spectroscopy. Furthermore, the selection can also be made depending on the excitation wavelength required for the measurement. Furthermore, the selection can take other sample characteristics into account. Overall, at least one coupling element is selected whose first coating and second coating match the measurement to be carried out.

Short description of the characters

• 1: eine Vorrichtung zur Spektroskopie;
• 2: eine Draufsicht auf ein Kopplungselement einer Vorrichtung zur Spektroskopie;
• 3: eine weitere Vorrichtung zur Spektroskopie;
• 4: verschiedene Ausführungsformen von Kopplungselementen in einem Querschnitt in Dickenrichtung,
• 5: eine perspektivische Ansicht eines halbzylinderförmigen Kopplungselementes;
• 6: eine Draufsicht auf das Kopplungselement der 5.

It shows in a purely schematic representation:

• 1 : a device for spectroscopy;
• 2 : a top view of a coupling element of a spectroscopy device;
• 3 : another device for spectroscopy;
• 4 : different embodiments of coupling elements in a cross section in the thickness direction,
• 5 : a perspective view of a semi-cylindrical coupling element;
• 6 : a top view of the coupling element 5 .

Preferred Embodiments

1 shows a device 10 comprising an excitation source 17, a detector 18 and a coupling element 11.

The coupling element 11 is in 1 shown in a cross section in the thickness direction. It has a first flat surface 12, which is divided into two areas, namely a first area 13 and a second area 14. A first coating 19 is applied to the first area 13, while a second coating 20 is applied to the second area 14.

Furthermore, the coupling element 11 has a second surface 15, which is in 1 Example shown is hemispherical and its apex is in direct contact with a sample 50.

The excitation source 17 sends excitation light towards the coupling element 11, which is spectrally selected by the first coating 19 and directed through the first region 13 of the coupling element 11 towards the sample 50. Light excited in the sample 50 is directed through the second region 14 and the second coating 20 towards the detector 18.

The second surface 15 can have a third coating 21 in order to positively influence the light deflection. The coupling element 11 has an extension 16 in a direction parallel to the first surface 12.

2 shows a top view of a hemispherical coupling element 11 of a device 10, namely of the first surface 12, which is divided into the first area 13 and the second area 14. Furthermore, the first coating 19 and the second coating 20 can be seen.

In 3 Another device 10 for spectroscopy can be seen, with the excitation source 17 and the detector 18 not being shown. The device 10 has a plurality of coupling elements 11, which are arranged in the form of an array 40. The array 40 has in particular columns that are in a first direction 41, and lines extending along a second direction 42.

Each coupling element 11 has a first surface 12, which is divided into a first area 13 with a first coating 19 and a second area 14 with a second coating 20.

The first coating 19 of the different coupling elements 11 is selected so that they differ between coupling elements that are adjacent in a first direction 41, while remaining constant in the lines that extend in the second direction 42. The second coating 20, on the other hand, is different between two coupling elements that are spaced apart in the second direction 42, but is constant along the first direction 41. In other words, the selection of the excitation wavelength changes along the first direction 41, while the selection of the wavelength to be detected changes in the second direction 42.

In 4 Various embodiments of coupling elements 11 are shown. In other words shows 4 different geometric shapes of coupling elements 11. The coupling elements 11 in 4 are shown in a cross section in the thickness direction 60.

A coupling element 11 with a spherical second surface 15 is shown under section a. The first surface 12 is the flat surface of the hemisphere formed by the coupling element 11, while the second surface 15 is hemispherical.

In section b, the coupling element 11 is geometrically composed of a hemisphere and a cylinder. The first surface 12 is formed as the end face of the cylinder, while the hemisphere adjoins the other end face of the cylinder. In cross section it can be seen how the second surface 15 is hemispherical, while a third surface 22 is designed as a lateral surface of the cylinder. The third surface 22 connects the first surface 12 with the second surface 15. In other words, section b shows a hemispherical shape with a distance to the first surface 12 formed by the cylinder.

In section c a coupling element 11 is shown, the second surface 15 of which describes the shape of a parabola in cross section. The coupling element 11 is thus formed as a capped ellipsoid, in other words as a capped egg, with the first surface 12 forming the cut surface.

In section d, a coupling element 11 is shown, which is formed analogously to the coupling element 11 of section a, but the hemispherical shape is cut off, so that the second surface 15 is flat and formed parallel to the first surface 12. The third surface 22 forms the truncated hemispherical shape.

In section e, the coupling element 11 is formed as a truncated pyramid. The second surface 15 is thus formed flat and parallel to the first surface 12. The third surface 22 forms the lateral surface of the pyramid.

In sections f and g, the coupling element 11 is formed as pyramids with different points, with the first surface 12 again forming the flat surface and the second surface 15 enclosing the lateral surface including the tip of the pyramid.

5 shows a perspective view of a semi-cylindrical coupling element 11. It has a first flat surface 12, which is divided into a first area 13 and a second area 14. A first coating 19 is applied to the first area 13 and a second coating 20 is applied to the second area 14. The coupling element 11 also includes a second surface 15, which can be brought into direct contact with a sample 50.

In 6 is a top view of the coupling element 11 5 shown. The first surface 12 is rectangular. It is divided into the first area 13 and the second area 14, with the first coating 19 being arranged in the first area 13 and the second coating 20 being arranged in the second area 14.

Reference symbol list

10

contraption

11

Coupling element

12

first surface

13

first area

14

second area

15

second area

16

expansion

17

source of stimulation

18

detector

19

first coating

20

second coating

21

third coating

22

third area

40

array

41

first direction

42

second direction

50

sample

60

Thickness direction

Claims (13)

Device (10) for spectroscopy of a sample (50), the device (10) comprising an excitation source (17) and at least one detector (18), the device (10) having at least one coupling element (11) with a first surface (12 ) and a second surface (15), the first surface (12) being flat, the first surface (12) having a first region (13) for coupling excitation light in the direction of the sample (50) and a second region ( 14) for coupling out light excited in the sample (50) in the direction of the detector (18), and wherein the first area (13) and the second area (14) differ, the device (10) comprising a plurality of coupling elements (11 ), characterized in that the coupling elements (11) are arranged as an array (40). Device (10) after Claim 1 , characterized in that the second surface (15) is curved. Device (10) after Claim 1 or 2 , characterized in that the coupling element (11) has the shape of a hemisphere, a half-cylinder, a cone or a pyramid. Device (10) according to one of the preceding claims, characterized in that the coupling element (11) has the shape of a truncated hemisphere, a truncated cone or a truncated pyramid. Device (10) according to one of the preceding claims, characterized in that the coupling element (11) has a spatial extent of less than 20 µm, preferably less than 15 µm, most preferably less than 10 µm. Device (10) according to one of the preceding claims, characterized in that the first region (13) of the first surface (12) comprises a first coating (19), the first coating (19) being designed as an optical filter. Device (10) according to one of the preceding claims, characterized in that the second region (14) of the first surface (12) comprises a second coating (20), the second coating (20) being designed as an optical filter. Device (10) after Claim 6 or 7 , characterized in that the second surface (15) comprises a third coating (21), wherein the third coating (21) differs from the first coating (19) and / or the second coating (20). Device (10) after Claim 1 , characterized in that the device (10) has a first and an adjacent second coupling element (11), the first and second coupling elements (11) each having a first coating (19) on the first region (13) of the first surface ( 12) and/or a second coating (20) on the second region (14) of the first surface (12), wherein the first coating (19) of the first coupling element differs from the first coating (19) of the second coupling element and/ or the second coating (20) of the first coupling element differs from the second coating (20) of the second coupling element (11). Device according to one of the Claims 1 or 9 , characterized in that the coupling elements (11) are arranged in rows and columns, the rows extending in a first direction (41) while the columns extend in a second direction (42), the first coatings (19) of the coupling elements (11) are selected so that the first coatings (19) differ between coupling elements (11) adjacent in the first direction (41), while the first coatings (19) of the coupling elements (11) are identical in a same row , and/or wherein the second coatings (20) of the coupling elements (11) are selected such that the second coatings (20) differ between coupling elements (11) adjacent in the second direction, while the second coatings (20) of the coupling elements ( 11) are identical in a same column. Device (10) according to one of the Claims 1 , 9 or 10 , characterized in that each coupling element (11) is assigned its own detector (18). Device (10) according to one of the Claims 1 , 9 or 10 , characterized in that the coupling elements (11) are assigned a common detector (18). Method for spectroscopy of a sample (50), the method comprising emitting excitation light by means of an excitation source (17), the excitation light passing through a respective first region (13) of a respective first flat surface (12) of a plurality of coupling elements (11) onto the Sample (50) is directed, light excited in the sample (50) being directed through a second area (14) of the first surface (12) towards a detector (18), the first area (13) and the second Distinguish area (14) of the first surface (12), characterized in that the coupling elements (11) are arranged as an array (40).

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