DE102020006998A1 - Method for cultivating a plant with at least one antiviral active ingredient, and use of the plant ingredient itself - Google Patents

Abstract

Method for cultivating a permanent culture of a plant from the Artemisia genus with at least one antiviral active ingredient, and subsequent active ingredient extraction, according to the preamble of claims 1 and 8 ff. In order to improve a method for cultivating this plant with at least one antiviral active ingredient in such a way that, if possible consistent active ingredient quality and quantity resulting from this, it is proposed according to the invention that the plant is first sorted for varietal purity and then propagated in a genetically stable and homogeneous manner by cuttings or meristem propagation and then cultivated as a varietal, that the cultivation is carried out indoors in greenhouses.

Description

The invention relates to a method for cultivating a plant with at least one antiviral active substance and plant ingredient itself, the plants being cultivated in a culture system with automatic irrigation and light tracking, according to the preamble of patent claims 1 and 8 et seq.

In the course of the often pandemic spread of viral infections, e.g. influenza and influenza-related virus strains such as Corona or SARS-CoV-2, various plant active ingredients have been tested in the past. This also includes the plant Artemisia Annua, which has already been advertised as possibly having a healing effect or healing support against the Covid-19 disease caused by the corona virus. It has become known https://www.tagesspiegel.de/berlin/klische-studie-zubeifuss-kraut-gegen-covid-19- wird-in-mexikogetestet/26144790.html

that a research group in Berlin has shown that ethanolic extracts from the Artemisia Annua plant are effective against the corona virus at the cell level.

This drug is therefore primarily about killing the virus. The said plant active ingredient is called artemisinin.

The procurement of this plant with consistent quality in terms of the ingredients is a problem. On the one hand, because the plant, which is given the trivial name annual mugwort, is, as I said, annual, and therefore sees changing, weather-related culture conditions outdoors and therefore in your spectrum of ingredients is swaying.

The invention is therefore based on the object of improving a method for cultivating this plant with at least one antiviral active ingredient in such a way that the quality and quantity of active ingredient are as constant as possible. Various forms of administration of this active substance are also specified.

The stated object is achieved according to the invention by the characterizing features of patent claim 1 in a method of the generic type.

Further advantageous refinements of the method are specified in the dependent claims 2 to 14.

Appropriate applications are specified with regard to administration of an active substance in the case of treatment with the active substance artemisinin.

The core of the invention is that the plant is first sorted for varietal purity and then propagated in a genetically stable and homogeneous manner by cuttings or meristem propagation and then cultivated as a varietal, that the cultivation is carried out indoors in greenhouses.
Here, a plant cultivation measure is carried out, in which the result is a different culture than in the field. Due to the distribution and dispersal properties of mugwort pollen, it will not be possible to grow a single-variety culture with a constant quality of ingredients outdoors.
In addition, generative propagation by allowing flowering leads to an annual culture.

This is exactly what should be avoided by purely vegetative propagation by cutting and planting cuttings. The culture thus becomes a permanent culture with cultures that remain the same and, above all, have previously been sorted according to type.

The selection of varietal purity at the start of the process is based on purely morphological characteristics, such as those that are decisive in varietal testing processes. By means of an accompanying measure of laboratory investigations, those varieties can then be identified by the morphological characteristics, which can be assigned to the morphological distinguishing characteristics in an analysis of the ingredients by an optimum of ingredients, e.g. but not exclusively a maximum artemisinin concentration.

In a further advantageous embodiment it is stated that the plant from the genus Artemisia is the species Artemisia Annua.

In a further advantageous embodiment, it is stated that the plant from the Artemisia genus is the Artemisia Lancea species.

In a further advantageous embodiment it is stated that the plant from the genus Artemisia is the species Artemisia Apiacea.

Furthermore, it is advantageously designed that the cultivation is operated all year round by means of heating and illumination by means of artificial light, which is tracked in the growth optimum.

Furthermore, it is advantageously designed that during cultivation, the plant illumination with hard UV wavelengths (380-440 nm) together with two Wavelengths in the red range (680-730 nm) are applied.

This measure can be used to influence the expression of the spectrum of ingredients, and thus the said formation of ingredients can be maximized.

In a further advantageous embodiment, it is stated that pure propagation of cuttings or meristems on the one hand suppresses varietal purity and on the other hand blooms.

Both measures together have the effect that the culture on the one hand remains varietal and on the other hand is taken out of the generative sexual reproduction and is made into a permanent culture, which is then operated all year round. The heating to a permanently good growth temperature and tracking with additional artificial light illumination ensure that the plant does not retreat due to the seasons.

In a further advantageous embodiment, it is stated that, in order to obtain the active ingredient artemisinin and accompanying other ingredients, plant matter in the culture is harvested simply by pruning the young shoots, and that the young shoots that have been cut off are then extracted with an extract containing all of the ingredients, in which artemisinin is contained in addition to other ingredients . This ensures a high level of active ingredient availability in the biomass.

In a further advantageous embodiment, it is stated that the extraction is carried out by crushing the fresh or dried young shoots using an alcoholic extract or a CO ₂ extract, or by drying at a drying temperature of ≦40° C., or by freeze drying. In this way, the active ingredients can be gently extracted.

The method follows the knowledge that the path from a corona virus infection to the outbreak of the disease Covid-19 depends on the amount of resorbed viruses. In other words, the severity of the disease depends on the amount of virus initially ingested.

Here, the active ingredient now intervenes in such a way that as many of the viruses as possible are killed immediately before they are resorbed accordingly.

This is achieved by the application forms of the amounts of active ingredient that can be reproduced with the indoor culture method according to the invention.
The amount of active ingredient is present in the plant substrate in a reproducibly high amount.
In the aftermath, the essential absorption areas such as mucous membranes can even be treated preventively.

Although mugwort is one of the main allergens and serves as a marker in the meteorological assessment of pollen load, this affects the pollen, not the young shoots harvested according to the invention. As mentioned above, the indoor method even suppresses generative propagation so that pollen does not form at all.

The said applications, which are placed on the different mucous membranes, achieve this effect.

• - Nasensprays
• - Sprays mit dem Extrakt zum Aufsprühen in die Mund-Nasen-Schutzmasken
• - Rachensprays mit dem Extrakt
• - Genussmittel mit dem Extrakt
• - Getränke mit dem Extrakt
• - Lutschtabletten mit dem Extrakt
• - Raumluftdispenser mit Kaltvernebelung zusammen mit dem Extrakt

Application forms of the extract should therefore be:

• - nasal sprays
• - Sprays with the extract for spraying on the mouth and nose protective masks
• - Throat sprays with the extract
• - stimulants with the extract
• - Drinks with the extract
• - Lozenges with the extract
• - Room air dispenser with cold nebulization together with the extract

Embodiments of the invention are shown in the drawing and described in more detail below.

With all of these uses, the aim is not primarily to kill the virus in the body as a therapeutic agent, but to reduce the number of active germs at the entry levels to the body, i.e. where the virus is absorbed.

This uses the knowledge that the severity of a Covid-19 disease is significantly reduced by reducing the number of active viruses ingested.

1 shows that Artemesia anua 1, which is one year old per se, is not generatively propagated in the method according to the application, but vegetatively propagated by propagation/cutting from cuttings. From these, a selection can be developed, if necessary with the inclusion of content analyses. These are then taken into culture 3, preferably in a greenhouse. The propagation is renewed again and again within a year, and cuttings are produced in this way. A perennial plant is thus in practice a permanent crop.

Temperatures between 18 and 30 °C are permanently set during cultivation, and if necessary with night setback.

Furthermore, blue wavelengths (400 and 440 nm) in particular are irradiated onto the culture by additional LED lighting. This serves the particularly stimulated synthesis of artemisinin.

This active ingredient is one of the secondary plant substances. Chemically it is a sesquiterpene. Artemisinin therefore has a peroxide structure and forms free radicals in the vicinity of iron, i.e. also in the area of the erythrocytes. This results in the killing of parasites.

In the present case, the aim was to have the active substance available all year round and to simultaneously stimulate the active substance synthesis in the plant, for which the above-mentioned measures are important.

In order to always harvest a high concentration of this active ingredient, preferably young shoots 4 of the Artemisia of the cuttings taken into culture are regularly harvested. These young shoots 4 form the harvest, which is then fed to an extraction 5, i.e. the active ingredients are extracted by alcoholic extracts, by CO2 extracts, acetic acid extracts, or extracts with glycerol or, if necessary, by creating a pressed plant juice.

The extract obtained in this way is then mixed with other active ingredients in a mixer.

These can be aspirin, ascorbic acid, but also amino acids such as lysine and valine.

Both increase the absorption of the actual active ingredient.

reference list

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plant

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cuttings

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Culture

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Harvest_young shoots

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extraction

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mixture

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Other active ingredients

Claims (16)

Method for cultivating a permanent culture of a plant from the Artemisia genus with at least one antiviral active ingredient, and subsequent active ingredient extraction, characterized in that the plant is first sorted for varietal purity and then propagated in a genetically stable and homogeneous manner by cuttings or meristem propagation and then cultivated varietal that the cultivation is carried out indoors in greenhouses. procedure after claim 1 , characterized in that the plant from the genus Artemisia is the species Artemisia Annua. procedure after claim 1 , characterized in that the plant from the genus Artemisia is the species Artemisia Lancea. procedure after claim 1 , characterized in that the plant from the genus Artemisia is the species Artemisia Apiacea. Procedure according to one of Claims 1 until 4 , characterized in that the cultivation is operated all year round by means of heating and illumination by means of artificial light, which is tracked in the growth optimum. Procedure according to one of Claims 1 until 5 , characterized in that during cultivation the plant illumination is exposed to hard UV wavelengths (380-440 nm) together with two wavelengths in the red range (680-730 nm). Procedure according to one of Claims 1 until 6 , characterized in that pure cuttings or meristem propagation on the one hand the varietal purity and on the other hand the flowering is suppressed. Method according to one of the preceding claims, characterized in that for the production of the active ingredient artemisinin and accompanying other ingredients plant matter in the culture can be harvested only by pruning the young shoots, and that the cut off young shoots then a total ingredient extract, in which Artimisinin is contained in addition to other ingredients, is extracted. procedure after claim 8 , characterized in that the extraction is carried out by crushing the fresh or dried young shoots using an alcoholic extract or a CO ₂ extract, or by drying at a drying temperature ≤ 40°C, or freeze drying. Use of the method according to the claims 1 until 9 produced active ingredient extract, characterized in that the extract is used in nasal sprays. Use of the method according to the claims 1 until 9 active ingredient extract produced, characterized in that the extract is used in sprays for spraying on the mouth and nose protective masks. Use of the method according to the claims 1 until 9 produced active ingredient extract, characterized in that the extract is used in throat sprays. Use of the method according to the claims 1 until 9 produced active ingredient extract, characterized in that the extract is used in luxury foods. Use of the method according to the claims 1 until 9 produced active ingredient extract, characterized in that the extract is used in beverages. Use of the method according to the claims 1 until 9 produced active ingredient extract, characterized in that the extract is used in lozenges. Use of the method according to the claims 1 until 9 produced active ingredient extract, characterized in that the extract is used in room air dispenser with cold nebulization.

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