DE102019116470A1 - Grape seed extract (TKE) with increased positive biological effectiveness, process for its production and its uses - Google Patents

Abstract

The invention relates to a new grape seed extract (TKE) with increased positive biological effectiveness, a method for its production and uses of the new grape seed extract (TKE) with increased positive physiological effectiveness due to very high oligonucleotide proanhocyandine content (OPC), especially in the presence of grape seed oil. The invention also relates to a method for obtaining a grape seed extract (TKE), also commonly known colloquially as OPC (oligomeric proanthocyanidins), which is characterized in that it is the extraction of grape seeds by sieving pomace from wine production and grinding the grape seeds obtained by sieving Grape seeds to an enriched, in particular to a highly enriched, grape seed extract (containing OPC) with an average particle size of at most 2000 microns (10 mesh), in particular with an average particle size of at most 1000 microns (18 mesh); with the additional proviso that the process does not include extraction with solvents or solvent mixtures. The invention also relates to a grape seed extract (TKE) (containing OPC, oligomeric proanthocyanidins), preferably obtained by a method according to the invention, wherein (i) the OPC content (oligomeric proanthocyanidins) in the grape seed extract (TKE) obtained (containing OPC) is at least 10 wt. -%, preferably at least 50% by weight, based on the dry matter of the grape seed extract (TKE) (containing OPC) as 100% by weight; and / or where (ii) the fat content in the grape seed extract (TKE) obtained (containing OPC) is at least 2% by weight, based on the dry matter of the grape seed extract (TKE) (containing OPC) as 100% by weight. The grape seed extract (TKE) according to the invention (containing OPC) can be present as a mixture with one or more substances from the group Damiana, Tadalfil, glycerin, emulsifier, pharmaceutical carriers, pharmaceutical auxiliaries, rose water, wheat germ oil and evening primrose oil. Compositions containing a grape seed extract (TKE) according to the invention (containing OPC) can be a food or a food additive, a food supplement, an animal feed additive, a cosmetic or a cosmetic additive, or an aphrodisiac.

Description

The invention relates to a new grape seed extract (TKE) with increased positive biological effectiveness, a method for its production and uses of the new grape seed extract (TKE) with increased positive physiological effectiveness.

Grape seed extract (TKE) can be obtained from grape seed flour, sometimes also called grape seed powder. Grape seed flour is a dark brown flour that is obtained from the seeds (kernels) of grapes, previously mostly (French) red wine kernels. Grape seed extract (TKE) with enriched oligonucleotide proanhocyandine (OPC) content can only be obtained by chemically extracting grape seed powder with ethanol, ethanol / water and / or mixtures with acetic acid derivatives or even chloroform with subsequent spray drying or the use of a drying roller or fluidized bed dryer.

For the previous production of grape seed flour according to the state of the art, the grape seeds are obtained from the pressing residue, the pomace, after the pressing process for winemaking. The pomace usually used as a raw material (hand-picked, which still has to be used on steep slopes) still contains the grape stalks, some leaves and the berry pods. A “destemming machine” (rotary sieve) first separates the kernels from the berry skin and stems in a first step. However, this rotary sieve separation only delivers a maximum of about 70% kernels with 20% shells and still a lot of stem remains. The loosely enriched kernels are then usually (uncontrolled) dried with warm or hot air and cleaned again with an air sifter or an old grain winch so that the pure kernels (now around 90% kernels) remain. Then the kernels are dried again (uncontrolled). The dried seeds are then cold-pressed in order to obtain grape seed oil from them. After pressing, the press cake remains, which is crushed by a screw conveyor, processed into granulate by a rolling mill and finally ground very finely into grape seed flour in a grain mill or vortex mill. The heat development can be up to 90 ° C to 100 ° C, whereby this temperature is only reached for a short time.

The special thing about the nutty grape seed flour is the high occurrence of OPC (oligomeric proanthocyanidins), a secondary plant substance with the strongest known antioxidant effect. The antioxidant capacity of a food can be described with the ORAC value (Oxygen Radical Absorption Capacity). Thanks to its high OPC content, grape seed flour is one of the foods with the highest ORAC value: 100,000 µmol TE / 100g.

The grape seed flour obtained according to the above state of the art, ie from the grape seeds by pressing out grape seed oil, only has a fat content of up to a maximum of about 4.32 g, is gluten-free, lactose-free and contains over 50% dietary fiber, as well as the following nutritional value -Table shows: Average nutritional values per 100 g Calorific value 1,129 kJ / 273 kcal Water content 6.1 g Dry matter 93.9 g protein 10.4 g fat 4.32 g Carbohydrates (without fiber) 20.0 g sugar 5.7 g Fiber 56.6 g ash 2.7 g Grapeseed flour is well suited for use in food. The special thing about grapeseed flour when used in food is that it retains most of its valuable ingredients when heated, with the exception of the OPC, which polymerize when heated too much and for too long. Unfortunately, the oligomeric proanthocyanidins polymerize when heated (water is lost) to polymeric proanthocyanidins and can then no longer be absorbed by the animal or human organism. Nevertheless, baking with grape seed flour is of course healthy, although the OPC is largely lost in the process. Apart from this disadvantage of polymerizing OPC, grape seed flour is very good for baking, but only proportionally (5 to 10%) to conventional flours. It gives all baked goods and dishes a hearty and hearty taste. Grape seed flour is also suitable for breading or flouring meat and fish. Ready-made baking mixes for grape seed bread, flour mixes with spelled flour and various pasta with grape seed flour are also known on the food market.

Grapeseed flour is also well suited for use as a dietary supplement. For example, pure grape seed flour can be used be stirred into smoothies or juices (e.g. about 1 teaspoon per serving) or in the form of grape seed capsules. In the case of chasubles on the market, however, OPC-enriched product, i.e. extracted product, is used, often together with vitamin C or other food supplements. It can also be consumed as an addition to muesli or yogurt. However, it should not be taken together with dairy products, as the protein contained in dairy products can reduce the antioxidant effect of the OPC.

In addition, grape seed extract is also suitable for use in animal feed. In the animal feed sector, grape seed flour is used in particular because of its valuable nutrients and active ingredients and is processed as a feed additive.

Grape seed extract can be extracted from the grape seed flour described above by extraction with water and / or ethanol, but i.a. also using chloroform, grape seed extract can also be obtained from grape seeds. In the grape seed extract obtained, the OPC content (oligomeric proanthocyanidins) is greatly increased because the extractant in the extraction cake leaves the insoluble components (e.g. stalk remains, leaf remnants, etc.) and the polymers proanthocyanidins and only the oligomeric proanthocyanidins (OPC) accumulate in the extract. As a result, the positive effects described above for grape seed flour can also be advantageous for the grape seed extract, in particular for use in food and / or for use as a dietary supplement, or other uses in the human area, e.g. dietetic and / or cosmetic applications, are provided in an effective manner.

The grape seed extract obtained in the prior art by extraction with solvents from grape seed flour, however, still has some disadvantages which must be remedied within the scope of the present invention. In particular, it is important to obtain a grape seed extract with the highest possible content of OPC (oligomeric proanthocyanidins) from the grape seeds used, without excessive heating and in an energy-efficient manner, preferably essentially the entire content of grape seed extract, including OPC (oligomeric proanthocyanidins), physiologically available from the grape seeds used close. As already mentioned, the oligomeric proanthocyanidins are biologically available, but not the polymeric proanthocyanidins, which are formed to a greater extent, for example, by excessive heating, drying too quickly or drying at too high a temperature.

The problem is solved by the process specified in the claims, in which grape seed extract (TKE) with a very high OPC content, which, together with larger proportions of grape seed oil ("fat"), is produced in an energy-saving manner.

The grape seed extract (TKE) obtained here according to the invention has, in addition to the OPC content and the proportions of grape seed oil (“fat content”), after drying as described here, usually a proportion of up to about 6% by weight of water (residual water) , for example, a proportion of about 1 wt .-% up to about 6 wt .-% water (residual water). This proportion of remaining water results from the im water supplied to the method according to the invention, the water supplied in the method ensuring that only shorter oligomers were extracted from OPC.

The proportion of grape seed oil in the grape seed extract (TKE) of the present invention is preferably in the range from about 10% by weight to about 15% by weight, based on the total grape seed oil-containing and OPC-containing grape seed extract (TKE) as 100% by weight .-%, determined by HPLC analysis (as explained in more detail below, for example).

Grapeseed oil is a vegetable oil that can be obtained from the seeds (kernels) of grapes. In the prior art, it is obtained from the cores by hot or cold pressing, but it can also be extracted chemically. The press residue from cold pressing is processed into grape seed flour. In the context of the present invention, however, the extraction of the grape seed extract (TKE) is carried out via a new process in such a way that the grape seed oil of the grape seeds used - in contrast to the extraction of grape seed extracts by the extraction method of the prior art via a grape seed flour, essentially in the extracted according to the invention Grape seed extract (TKE) remains.

The content of grape seed oil in the grape seed extract according to the invention (TKE) obtained then essentially also represents the fat content of the grape seed extract (TKE) according to the invention. Accordingly, the fat content in the grape seed extract according to the invention (TKE) obtained is also in the range from about 10% by weight to about 15% by weight % By weight based on the total grape seed oil-containing and OPC-containing grape seed extract (TKE) as 100% by weight.

The invention relates generally to a new method for obtaining a grape seed extract (TKE), generally (popularly) already called OPC as such, which is characterized in that it is the extraction of grape seeds by sieving pomace according to the invention, preferably by a harvester or the harvest Downstream destemming machine grapes that have already been destemmed and, after subsequent, in particular precisely, controlled drying, grinding of the grape seeds obtained by sieving into an enriched, preferably a highly enriched, grape seed extract (TKE) with (very) high OPC content with an average particle size of at most 2000 microns (10 mesh), in particular with an average particle size of at most 1000 microns (18 mesh), preferably with an average particle size of at most 250 microns (60 mesh); with the additional proviso that the process does not include extraction with solvents or solvent mixtures. The invention also relates to a grape seed extract (OPC, oligomeric proanthocyanidins), preferably obtained according to the present process according to the invention, wherein (i) the OPC content (oligomeric proanthocyanidins) in the grape seed extract (TKE) obtained is at least 10% by weight, based on the dry matter of the grape seed extract (TKE) as 100% by weight; and / or where (ii) the fat content in the grape seed extract (TKE) obtained is at least 2% by weight, but mostly well over 10%, based on the dry matter of the grape seed extract (TKE) as 100% by weight. The grape seed extract (TKE) according to the invention can be present in a mixture with one or more substances from the group Damiana, Tadalfil, glycerin, emulsifier, pharmaceutical carriers, pharmaceutical auxiliaries, rose water, wheat germ oil and evening primrose oil. Compositions containing a grape seed extract (TKE) according to the invention can be a food or a food additive, a food supplement, an animal feed additive, a cosmetic or a cosmetic additive, or an aphrodisiac.

The invention thus generally relates to a method for obtaining a grape seed extract (TKE), here referred to as "TKE" in the context of the invention, generally referred to the important component OPC, often colloquially called OPC (oligomeric proanthocyanidins), which is characterized in that it the extraction of grape seeds by sieving pomace, preferably pomace from grapes de-stemmed from the winemaking process and grinding the grape pips obtained by fine sieving with subsequent precisely controlled drying to an enriched, preferably a highly enriched, grape seed extract (TKE) with very high OPC content and with an average particle size of at most 2000 microns (10 mesh), in particular with an average particle size of at most 1000 microns (18 mesh), preferably with an average particle size of at most 250 microns (60 mesh); with the additional proviso that the process does not include extraction with solvents or solvent mixtures.

The stipulation applicable according to the invention that the process does not include extraction with solvents or solvent mixtures advantageously influences the composition of the grape seed extract obtained, which is generally also called OPC (oligomeric proanthocyanidins).

The terms “grape seed extract” (TKE), “grape seed flour”, “grape seed oil” and “OPC” (“oligmers proanocyanidins”) are to be distinguished from one another within the scope of the present invention and thus have different meanings.

The meaning of the term “grape seed extract” (TKE) in the context of the present invention will be explained further below. In popular usage, grape seed extract (TKE) is already called OPC, regardless of the content of oligonucleotides proanthocyanidins. In the context of the present invention, grape seed extract is called “TKE”, and the content of oligmeric proanocyanidins therein is called “OPC”. The inventive combination of the use of pomace from destemmed grapes with the sieving technique described together with the very gentle, precisely controlled and rapid drying, the use of extraction agents, the filtering off of insoluble components (extraction cake) and the subsequent drying of the extract obtained in this way (for Example with spray drying or drying rollers) can be avoided. In addition, the process according to the invention provides a TKE with a very high (up to 100% of the original content in grape seeds) OPC content in combination with an additional high content of grapeseed oil (also called fat content, containing many unsaturated fatty acids) that is produced by the process of State of the art with extraction followed by spray drying (or other drying) due to the thermal load and certainly not in combination with grapeseed oil, since when using extraction agents either grape seed flour is already used as the raw material from which the grapeseed oil was pressed or else the extractants leave the grape seed oil in the grapeseed flour, as the grapeseed oil is not extracted. The term “grape seed flour” is understood as follows in the context of the present invention. Grape seed flour (also grape seed powder) is a dark brown flour that is obtained from the seeds (kernels) of grapes. The special thing about the nutty grape seed flour is the high occurrence of OPC, a secondary plant substance with an antioxidant effect. The antioxidant capacity of a food can be described with the ORAC value (Oxygen Radical Absorption Capacity). Thanks to its high OPC content, grape seed flour is one of the foods with the highest ORAC value: 100,000 µmol TE / 100g.

The term “grape seed oil” is understood as follows in the context of the present invention. Grapeseed oil is a vegetable oil that is obtained from the seeds (kernels) of grapes. It can be extracted from the kernels by hot or cold pressing, but it can also be extracted chemically. The pressing residue from cold pressing is processed into grape seed flour.

The meaning of the term “OPC” (“Oligmere Proanocyanidins”) in the context of the present invention is to be explained further below. Oligomeric proanthocyanidins, also oligomeric procyanidins, OPC or PCO (oligomeric proanthocyanidins), are naturally occurring substances in plants that belong to the group of flavanols and can be assigned to the higher-level polyphenols. OPC are mostly dimers or trimers of catechins. The solubility in water decreases very sharply with the length of the "mers".

In the context of the present invention, the term “enriched” means a minimum content of OPC of about 10% by weight, up to an OPC content of about <50% by weight, based on the total grape seed extract (TKE) as 100% by weight .-%.

In the context of the present invention, the term “highly enriched” or “highly dosed” means a minimum OPC content of approximately 50% by weight, up to a maximum OPC content of approximately 80% by weight or even more than 100% by weight, based on the total grape seed extract (TKE).

This means that the OPC (oligonucleotide proanthocyanidins) naturally contained in the grape seed extract according to the invention is at least more than 10% (w / w) "enriched", preferably at least more than 50% (w / w) .) Is “highly enriched” or “highly dosed”, more preferably at least about 80% (w / w) or even more “highly enriched” or “high dosed” is ”. At least more than 80% (w / w), at least more than 90% (w / w), at least more than 95% (w / w), or 100% (w / w), each based on the OPC (oligonucleotide proanthocyanidins) naturally contained in the untreated grape seeds as 100% by weight, as OPC (oligonucleotide proanthocyanidins) for the body of an animal subject, preferably a mammal, and particularly preferred for a person, made physiologically available in grape seed extract (TKE).

Another important feature of the present invention is that, in contrast to the extraction processes of the prior art, the grape seed extract (TKE) according to the invention still contains significant amounts of valuable grape seed oil. The presence of grape seed oil shows up in an increased fat content compared to grape seed flour, for example a fat content of at least about 4.4 g, preferably of at least about 4.5 g, more preferably of at least about 5 g, each based on 100 g of grape seed extract (TKE), the fat content up to Fat content of the grape seeds used to obtain the grape seed extract (TKE) according to the invention can come close. Grape seed extract (TKE) according to the invention can therefore not only be distinguished from grape seed extracts of the prior art by the OPC content, but also by its increased fat content compared to grape seed extracts of the prior art. In addition to the fat content, the grape seed extract (TKE) according to the invention also contains a composition of the fat content which essentially corresponds to the natural composition of grape seed oil, as indicated in the table below. Each of the constituents given in the table below can characterize a grape seed extract (TKE) according to the invention independently of one another, both individually and in any combination with one another. Grape seed extracts according to the invention can preferably be characterized by the unsaturated fatty acids, independently of one another, individually or in any combination with one another, such as oleic acid, linoleic acid and / or linolenic acid, and also by the sum of the content of mono and / or unsaturated fatty acids. The following weight data are based on the grape seed oil contained in the grape seed extract (TKE) as 100% by weight, each determined by HPLC analysis. Grapeseed oil ingredients Fat content (% by weight, based on grape seed oil as 100% by weight): Oleic acid Around 14-24 Linoleic acid About 63 - 75 Linolenic acid About 0.2 Palmitic acid About 5.5-11 More fatty acids About 2-5 stearic acid Total saturated fat About 11 Total of monounsaturated fatty acids About 19 Total polyunsaturated fatty acids About 70 Vitamin ingredients (based on 1g grape seed oil): Vitamin A Up to about 4.49 mg / g Vitamin E2 Up to about 2.1 mg / g Other ingredients: Lecithin, Procyanidin, Campesterol, Beta-Sitosterol, Stigmasterol

The following can be said about the analysis of fatty acids.

The modern qualitative and quantitative analysis of fatty acids in food chemistry and in physiological research usually makes use of chromatographic methods. Capillary gas chromatography (after transesterification to the methyl ester), HPLC and the coupling of these processes with mass spectrometry are used. Usually the fatty acids are in the form of suitable derivatives, such as. B. the fatty acid methyl esters or their TMS derivatives, separated by chromatography. In special cases, classic column and thin-layer chromatography is still used today; the isomers are separated by means of silver nitrate thin layer chromatography. The acid number (AN) can also be used for the analytical determination of quality. The acid number (AN) is a chemical quantity used to characterize acidic components in fats or oils. It describes the mass of potassium hydroxide (in mg) that is necessary to neutralize the free fatty acids contained in 1 g of fat. However, the acids chemically bound as acylglycerols (i.e. fatty acid esters) are not included, as is the case with the saponification number.

Grape seed extracts (TKE) according to the invention can therefore, in each case based on the grape seed oil contained in the grape seed extract (TKE) as 100% by weight, about 30% by weight to about 70% by weight, preferably about 50% by weight to about 70% % By weight, particularly preferably about 60% by weight to about 70% by weight, in total contain polyunsaturated fatty acids, each determined by HPLC analysis.

In each case based on the total grape seed oil-containing and OPC-containing grape seed extract (TKE) as 100% by weight, the grape seed extract (TKE) according to the invention can thus be about 3.0% by weight to about 10.5% by weight, preferably contain about 5.0% by weight to about 10.5% by weight, particularly preferably about 6.0% by weight to about 10.5% by weight, in total of polyunsaturated fatty acids, in each case determined by HPLC -Analysis.

Grape seed extracts (TKE) according to the invention can therefore, in each case based on the grape seed oil contained in the grape seed extract (TKE) as 100% by weight, about 5 to about 20% by weight, preferably about 10% by weight to about 20% by weight , particularly preferably about 15 wt .-% to about 20 wt .-%, contain a total of monounsaturated fatty acids, each determined by HPLC analysis, each based on the total grape seed oil-containing and OPC-containing grape seed extract (TKE) as The grape seed extract (TKE) according to the invention can contain 100% by weight, i.e. about 0.5 to about 3% by weight, preferably about 1.0% by weight to about 3% by weight, particularly preferably about 1.5% by weight. -% to about 3% by weight, in total, contain monounsaturated fatty acids, each determined by HPLC analysis.

Grape seed extracts according to the invention (TKE) can therefore, in each case based on the grape seed oil contained in the grape seed extract (TKE) as 100% by weight, about 30% by weight to about 75% by weight, preferably about 50% by weight to about 75% % By weight, particularly preferably about 60% by weight to about 75% by weight, contain linoleic acid, each determined by HPLC analysis.

In each case based on the total grape seed oil-containing and OPC-containing grape seed extract (TKE) as 100 wt .-%, the grape seed extract (TKE) according to the invention can thus be about 0.3 wt .-% to about 11.3 wt .-%, preferably about 5.0% by weight to about 11.3% by weight, particularly preferably about 6.0% by weight to about 11.3% by weight, contain linoleic acid, in each case determined by HPLC analysis. The detection and determination of the content of linoleic acid is usually carried out by gas chromatography of the methyl ester; In addition, the unsaturated isomers can be separated using silver nitrate thin-layer chromatography.

Grape seed extracts according to the invention (TKE) can therefore, in each case based on the grape seed oil contained in the grape seed extract (TKE) as 100% by weight, about 5% by weight to about 25% by weight, preferably about 10% by weight to about 25% % By weight, particularly preferably about 14% by weight to about 24% by weight, contain oleic acid, in each case determined by HPLC analysis. In each case based on the total grape seed oil-containing and OPC-containing grape seed extract (TKE) as 100% by weight, the grape seed extract (TKE) according to the invention can thus be about 0.5% by weight to about 3.8% by weight, preferably about 1.0% by weight to about 3.8% by weight, particularly preferably about 1.4% by weight to about 3.6% by weight, contain oleic acid, each determined by HPLC analysis. The detection and determination of the content of oleic acid as well as other fatty acids in fats is usually carried out by gas chromatography of the methyl ester; In addition, the unsaturated isomers can be separated using silver nitrate thin-layer chromatography. A second, unspecific detection method is the bromine water sample. The result is a disappearance of the brown color.

Grape seed extracts according to the invention (TKE) can therefore, in each case based on the grape seed oil contained in the grape seed extract (TKE) as 100% by weight, about 0.05% by weight to about 0.25% by weight, preferably about 0.1% by weight % to about 0.25% by weight, particularly preferably about 0.15% by weight to about 0.25% by weight, contain linolenic acid, each determined by HPLC analysis. Each related The grape seed extract according to the invention (TKE) can thus be about 0.005% by weight to about 0.04% by weight, preferably about 0.01%, on the total grape seed oil-containing and OPC-containing grape seed extract (TKE) as 100% by weight % By weight to about 0.04% by weight, particularly preferably about 0.015% by weight to about 0.04% by weight, contain linolenic acid, in each case determined by HPLC analysis. The detection and determination of the content of linolenic acid and its isomers is usually carried out by gas chromatography of the methyl ester; In addition, the unsaturated isomers can be separated using silver nitrate thin-layer chromatography. Grape seed extracts (TKE) according to the invention can also be used, based on the grape seed oil contained in the grape seed extract (TKE) as 100% by weight, about 5% by weight to about 12% by weight. -%, preferably about 5% by weight to about 11% by weight, of palmitic acid, each determined by HPLC analysis. In each case based on the total grape seed oil-containing and OPC-containing grape seed extract (TKE) as 100% by weight, the grape seed extract (TKE) according to the invention can thus be about 0.5% by weight to about 1.8% by weight, preferably contain about 0.5% by weight to about 1.65% by weight, palmitic acid, in each case determined by HPLC analysis.

Grape seed extracts (TKE) according to the invention can furthermore contain, in each case based on the grape seed oil contained in the grape seed extract (TKE) as 100% by weight, to about 5% by weight, preferably about 2% by weight to about 5% by weight, of stearic acid contained, each determined by HPLC analysis. In each case based on the total grape seed oil-containing and OPC-containing grape seed extract (TKE) as 100 wt .-%, the grape seed extract (TKE) according to the invention can thus up to about 0.75 wt .-%, preferably about 0.2 wt .-% contain up to about 0.75% by weight, stearic acid, in each case determined by HPLC analysis.

The invention can preferably relate to a method for obtaining a grape seed extract (generally called TKE) containing OPC (oligomeric proanthocyanidins), which is characterized in that
That it is the extraction of grape seeds by sieving non-destemmed or preferably destemmed pomace from winemaking and grinding the grape seeds obtained through targeted multiple sieving into an OPC-enriched, in particular a highly enriched, grape seed extract (TKE) with an average particle size of at most 2000 microns (10 mesh), in particular with an average particle size of at most 1000 microns (18 mesh), preferably with an average particle size of at most 250 microns (60 mesh); an average particle size of at most 60 mesh has proven to be advantageous and sufficient, especially when grape seed oil ("fat content") is present, which favors the absorption of the OPC in the body as a transmitter,
and or
that it comprises the extraction of grape seeds by sieving pomace from winemaking and fine grinding to give an OPC-enriched, in particular highly enriched, grape seed extract (TKE) with an average particle size of about 250 microns (60 mesh) at most;
and with the additional proviso that the process does not include extraction with solvents or solvent mixtures.

In the described method according to the invention for obtaining a grape seed extract (TKE), the average particle size can be at least> 250 microns (60 mesh) in the case of grinding the grape seeds obtained by sieving to an enriched, in particular to a highly enriched, grape seed extract (TKE).

A method according to the invention for obtaining a grape seed extract (TKE), e.g. As previously described, it may be preferred that it comprises grinding the grape seeds obtained by sieving to form an enriched, in particular a highly enriched, grape seed extract (TKE) with an average particle size of at most 1680 microns (12 mesh ), no more than 1410 microns (14 mesh), or no more than 1190 microns (16 mesh). It can preferably be characterized in that it comprises grinding the grape seeds obtained by sieving to form an enriched, in particular a highly enriched, grape seed extract (TKE) with an average particle size of at most 1000 microns (18 mesh), in particular at most 841 20 meshes or less, 25 meshes or less, 30 meshes or less, 500 microns (35 meshes) or less, 40 meshes or less, or 45 meshes or less (354 microns) , or no greater than 297 microns (50 mesh).

In the described method according to the invention for obtaining a grape seed extract (TKE), in the case of an (additional) fine grinding of the grape seeds obtained by sieving to an enriched, if desired to a highly enriched grape seed extract (TKE), the average particle size, if desired, at least 37 microns (400 mesh) , at least 44 microns (325 mesh), at least 53 microns (270 mesh), at least 63 microns (230 mesh), at least 74 microns (200 mesh), or at least 88 microns (170 mesh).

A method according to the invention for obtaining a grape seed extract (TKE), e.g. As previously described, it can be preferred that it comprises a fine grinding of the grape seeds obtained by sieving to form an enriched, in particular a highly enriched, grape seed extract (TKE) with an average particle size of at most 250 microns (60 mesh ). Average particle sizes lower than 250 microns (60 mesh) can also be produced, for example no more than 210 microns (70 mesh), no more than 177 microns (80 mesh), or no more than 149 microns (100 mesh). In this case of a fine grinding of the grape seeds obtained by sieving to form an enriched, in particular a highly enriched, grape seed extract (TKE), for example, the average particle size, if desired, can be at least 37 microns (400 mesh), at least 44 microns (325 mesh), at least 53 microns (270 mesh), at least 63 microns (230 mesh), at least 74 microns (200 mesh), or at least 88 microns (170 mesh). However, an average particle size less than about 250 microns (60 mesh) or less is not required in the present invention.

In one example of the method according to the invention for obtaining a grape seed extract (TKE), as described above, for example, it can be preferred that it comprises a fine grinding of the grape seeds obtained by sieving to form an enriched, in particular a highly enriched, grape seed extract (TKE) with an average particle size ranging from about 250 microns (60 mesh) to about 37 microns (400 mesh), 250 microns (60 mesh) to about 44 microns (325 mesh), 250 microns (60 mesh) to about 53 microns (270 mesh) ), 250 microns (60 mesh) to about 63 microns (230 mesh), 250 microns (60 mesh) to about 74 microns (200 mesh), or 250 microns (60 mesh) to about 88 microns (170 mesh). Average particle sizes lower than 250 microns (60 mesh) can also be made, for example 210 microns (70 mesh) to about 37 microns (400 mesh), 210 microns (70 mesh) to about 44 microns (325 mesh), 210 microns (70 mesh) to about 53 microns (270 mesh), 210 microns (70 mesh) to about 63 microns (230 mesh), 210 microns (70 mesh) to about 74 microns (200 mesh), 210 microns (70 mesh) to about 88 microns (170 mesh); or 177 microns (80 mesh) to about 37 microns (400 mesh), 177 microns (80 mesh) to about 44 microns (325 mesh), 177 microns (80 mesh) to about 53 microns (270 mesh), 177 microns (80 Mesh) to about 63 microns (230 mesh), 177 microns (80 mesh) to about 74 microns (200 mesh), 177 microns (80 mesh) to about 88 microns (170 mesh); or 149 microns (100 mesh) to about 37 microns (400 mesh), 149 microns (100 mesh) to about 44 microns (325 mesh), 149 microns (100 mesh) to about 53 microns (270 mesh), 149 microns (100 Mesh) to about 63 microns (230 mesh), 149 microns (100 mesh) to about 74 microns (200 mesh), or 149 microns (100 mesh) to about 88 microns (170 mesh); or in the range of about 149 microns (100 mesh) to about 44 microns (325 mesh), from about 149 microns (100 mesh) to about 53 microns (270 mesh), from about 149 microns (100 mesh) to about 63 microns ( 230 mesh), from about 149 microns (100 mesh) to about 74 microns (200 mesh), from about 149 microns (100 mesh) to about 88 microns (170 mesh); or that it comprises a fine grinding of the grape seeds obtained by sieving to give a fortified, in particular a highly fortified, grape seed extract (TKE) with an average particle size in the range from about 125 microns (120 mesh) to about 44 microns (325 mesh) of about 125 microns (120 mesh) to about 53 microns (270 mesh), from about 125 microns (120 mesh) to about 63 microns (230 mesh), from about 125 microns (120 mesh) to about 74 microns (200 mesh), from about 125 microns (120 mesh) to about 88 microns (170 mesh). However, an average particle size less than about 250 microns (60 mesh) or less is not required in the present invention.

The term "fine grinding" means that the grape seeds obtained by sieving are finely ground to an enriched, in particular a highly enriched, grape seed extract (containing OPC, oligomeric proanthocyanidins), with an average particle size of at most about 250 microns (60 mesh ).

According to the invention, fine grinding is particularly advantageous since it has been shown that the method according to the invention is particularly suitable for making all OPC (oligonucleotide proanthocyanidins) from the grape seeds available for the body of an animal subject, preferably a human, as already explained above to be able to. Without wishing to be tied to a particular theory or wishing to restrict the scope of the invention thereby, it is assumed that, because of the surprisingly high grape seed oil / fat content remaining in the grape seed extract (TKE) obtained according to the invention, a very high proportion, i.e. in particular in the amounts described above, of OPC (oligonucleotide proanthocyanidins) can be provided in a physiologically usable manner. According to the invention, the higher the fat content of the grape seeds used, the more “coarsely” you can grind in order to achieve the same physiological effectiveness compared to “finer” ground grape seeds with a lower fat content.

The fat content in the grape seeds used and in a grape seed extract (TKE) produced according to the invention (containing OPC, oligomeric proanthocyanidins) can be determined by the person skilled in the art using any of the known analytical methods. The following can be summarized for the analysis of the fat content.

The fat content of foods is usually determined by extraction with lipophilic solvents. The FDA (Food and Drug Administration, USA) defines fat, for example, as the saponifiable part of a food. This means that non-acylglycerides, such as sterols or phosphatides, do not fall under the FDA definition of fat. For fat characterization, titration-analytical indicators such as iodine number, Reichert-Meißl number, saponification number, peroxide number or acid number are determined. For the qualitative and quantitative determination of individual fat components, chromatographic methods are preferably used. The fatty acid distribution can be determined using gas chromatography. Accompanying fat substances such as zoo or phytosterols or lipophilic vitamins are also determined by gas chromatography or HPLC ("High Performance Liquid Chromatography"). For reliable identification of individual Components of the fats, mass spectrometry is mostly used in the coupling with gas chromatography or with HPLC.

Likewise, the person skilled in the art can determine the OPC content (OPC, oligomeric proanthocyanidins) in the grape seeds used and in a grape seed extract (OPC, oligomeric proanthocyanidins) produced according to the invention using any of the known analytical methods. For example, the OPC content can be determined according to the US patent publication US 8,383,413 B2 procedures described.

The US patent publication US 8,383,413 B2 provides a method for testing OPC contained in natural substances such as foods and beverages, pharmaceuticals and / or cosmetics, and can also be applied to grape seeds, grape seed flour and grape seed extract. A method for testing OPC is described in which anthocyanidin obtained by hydrolysis of OPC is tested in order to determine the total amount of OPC and in which the proportions of the degrees of polymerization of OPC are examined by high performance liquid chromatography (HPLC) to to determine the content of the respective polymers in OPC.

In the context of the present invention, high-performance liquid chromatography (HPLC) is the preferred and most reliable analytical method for determining the grape seed extract (TKE) and its components, such as in particular OPC and its content, grape seed oil and its content, and the fat content and fat composition, including the components of saturated, singly saturated and multiply saturated fatty acids, as already described above.

The OPC content in the grape seed extract according to the invention is preferably in the range from greater than about 50% by weight to about 80% by weight, particularly preferably in the range from greater than about 55% by weight to about 80% by weight, and very particularly preferably in the range of greater than about 60% by weight or about 65% by weight to about 80% by weight, in each case determined by HPLC analysis, and based on 100% TKE.

The term “grinding” means that the grape seeds obtained by sieving are ground to a fortified, in particular a highly fortified, grape seed extract (containing OPC, oligomeric proanthocyanidins).

The grinding or fine grinding usually takes place in a grinder known to the person skilled in the art, which is adjusted to the particle sizes or grain sizes of the grape seed flour produced to be achieved. It is preferred to use a type of mill in which the grist does not heat up too much, e.g. Air jet mills ("Jet Mills").

Flour is primarily understood to mean the powder that is created when the (possibly fine) grinding of plant grains, ie in the present case the grape seed flour produced from grape seeds. Grinder is the name for a functional unit or an aggregate for grinding (comminuting) substances in mills, e.g. B. in flour mills or oil mills. Modern grinders usually consist of metallic tools, possibly also of corundum. The grinding effect is achieved by multiple crushing in an increasingly narrowing grinding gap, which can usually be varied by axially displacing the rotor or stator. Milling is done by shearing and / or friction. Further grinding systems are roller mills, beater mills and impact mills. In roller mills, the material is crushed between cylindrical grooved or (rough) smooth rollers that rotate at different speeds (advance). In impact crushers the material for grinding is crushed by turning on or Mehrfachprallung by being covered by the very rapidly rotating grinding tools (up to 11,000 min ^-1 or 250 m / s). There are also mills with counter-rotating rotors, with rotor and stator and only with rotor. During grinding cycles, the grist is ground between the grinders, but care should be taken to avoid excessive heating.

In the context of the present invention, for example, a so-called jet mill can be used very advantageously, in which the grape seeds are sucked in together with a little air, greatly accelerated and crushed. This has the advantage that the grist almost does not heat up.

If, on the other hand, a method for obtaining grape seed extract with extraction is used in the prior art, the grain size does not play a role in contrast to the present invention, but then extraction may have to be longer to enrich OPC, which of course costs additional energy.

In the method according to the invention for obtaining a grape seed extract (TKE), in which grape seeds are obtained by sieving pomace from winemaking and fine grinding to give an enriched, in particular a highly enriched, grape seed extract (TKE), it may still be desirable that it comprises (additional) fine grinding to give a fortified, in particular a highly fortified, grape seed extract (TKE) with an average particle size of approximately at most 53 microns (270 mesh); with the additional proviso that the process does not include extraction with solvents or solvent mixtures.

The method according to the invention for obtaining a grape seed extract (TKE) can be characterized in that in the method the pomace fed to the sieve is sprinkled with a water mist at the sieve feed, and optionally the (moist) grape seeds obtained by sieving at a temperature of no more than about 60 ° C to obtain an enriched, especially a highly enriched, grape seed extract (TKE). The drying is preferably carried out gently and as evenly as possible based on the grape seed material to be dried. The (moist) grape seeds obtained by sieving are preferably used at a temperature of no more than about 55 ° C, more preferably at a temperature of no more than about 50 ° C, (gently and as evenly as possible) to obtain an enriched, in particular one highly-enriched, dried grape seed extract (TKE).

The method according to the invention for obtaining a grape seed extract (TKE) can also be characterized in that for obtaining an enriched, in particular a highly enriched, grape seed extract (TKE) (containing OPC), those grape seeds are used which comprise or consist of white wine grape seeds.

The method according to the invention for obtaining a grape seed extract (TKE), such as e.g. previously described, can in particular also be characterized in that preferably the white grape seeds from one or more of the grape varieties are selected from the group consisting of Silvaner, Müller, Riesling, Bacchus, Pinot Blanc, Pinot Gris, Scheurebe, Rislaner, Morio Muscat, Chardonnay, Traminer, Kerner and Sauvignon Blanc; preferably one or more of the grape varieties selected from the group consisting of Silvaner, Müller, Riesling and Bacchus.

The person skilled in the art understands that the grape seeds used, preferably the white wine grape seeds, can also consist of two or more grape varieties, that is to say of any combination of two or more of the same. Such a combination of grape seeds, preferably white wine grape seeds, takes into account, for example, the conditions that exist in practice, e.g. depending on how the grape harvest and processing takes place.

White wine is made from white or red berries with light pulp. The color of the berry skin can be light yellow, yellow-green, green, gray, gray-red and red. The pulp contains only small amounts of color. With the exception of the dye grape, a red wine variety, the coloring pigments of the grapes are only found in the berry skin. By squeezing the berry juice early, extraction of the components of the berry skin is largely avoided. Compared to the production of a white wine, the process of pressing red wine is technically not that complex. This fact is essential for the invention, since according to the invention preferably grape seeds from white wine grapes as starting material for the production of the grape seed extract according to the invention (TKE), such as e.g. previously described, can be used. It goes without saying that the process can also be applied to grape seeds from red wine grapes. The grape seed extracts (TKE) obtainable from grape seeds from red wine grapes, however, have a lower OPC content compared to grape seed extracts (TKE) from grape seeds from white wine grapes.

In contrast to white wine, red wine is a wine made from blue grapes. In addition to the type of grapes used, it also differs from white or rose wine in the manufacturing process. A red wine is made through maceration or digestion. In addition to the alcoholic fermentation of the colorless juice of the berry, certain components are extracted from the solid parts of the mash. The solid components of the berry mash are made up of the skin of the berries, the seeds and possibly the stems of the grape. In the classic manufacturing process, the desired components are extracted during the must fermentation, the duration of which can be determined individually. Another method uses mash heating prior to fermentation.

• - Silvaner, auch Sylvaner oder Grüner Silvaner, ist eine autochthone Weißweinsorte aus Österreich. Ihre größte Verbreitung hat sie in Deutschland. In ihrem Ursprungsland Österreich hat sie heute nur untergeordnete Bedeutung. Silvaner bringt bei guter Reife und nicht zu hohem Ertrag harmonische Weine mit feiner Säure und zarter Blume.
• - Bacchus oder Frühe Scheurebe ist eine Weißweinsorte, die eine Kreuzung von (Silvaner x Riesling) und Müller-Thurgau ist. Die Rebsorte wird vor allem in Deutschland und in England angebaut. Kleine Bestände sind auch in der Schweiz bekannt. Bacchus verdankt ihre Popularität unter Winzern besonders der Tatsache, dass sie - im Gegensatz zum Riesling - eine frühreifende Rebsorte ist, die sehr hohe Reifegrade erreicht und deshalb auch in Lagen angebaut werden kann, die für den Riesling weitgehend ungeeignet sind. Die aus ihr gewonnenen Weine sind aromatisch und fruchtig, aber nur, wenn das Lesegut völlig ausgereift ist. Allerdings fehlt es den Weinen manchmal an Säure, daher wird sie häufig mit Müller-Thurgau verschnitten.
• - Müller(-Thurgau) ist eine Weißweinsorte, die heute aus Rechts- und Marketinggründen auch Rivaner genannt wird. Die Traube ist mittel bis groß, locker- bis dichtbeerig, konisch, oft geschultert. Die Beeren sind mittelgroß, oval, gelblichgrün gefärbt und leicht beduftet. Das Beerenfleisch ist ungefärbt und saftig mit einem dezenten Muskatgeschmack.
• - Riesling ist eine weiße Reb- und Weinsorte, die zu den hochwertigsten und kulturprägenden Gewächsen gezählt wird. Die besten Rieslinge werden in klimatisch kühleren Weinbaugebieten erzeugt. Vor allem wird die Sorte in Deutschland, aber auch in zahlreichen anderen Weinbauländern angebaut. Riesling-Weine genießen hohes Ansehen auf internationalen Märkten. Viele Spitzenlagen sowohl in Deutschland als auch in anderen Weinbauländern sind mit Riesling bestockt.

The grape seeds preferably come from white wine grapes as the starting material from one or more of the grape varieties selected from the group consisting of Silvaner, Müller, Riesling and Bacchus:

• - Silvaner, also Sylvaner or Grüner Silvaner, is an autochthonous white wine variety from Austria. It is most widespread in Germany. In its country of origin, Austria, it is only of secondary importance today. With good ripeness and not too high yields, Silvaner produces harmonious wines with fine acidity and delicate flowers.
• - Bacchus or early Scheurebe is a white wine variety that is a cross between (Silvaner x Riesling) and Müller-Thurgau. The grape variety is mainly grown in Germany and England. Small stocks are also known in Switzerland. Bacchus owes its popularity among winemakers in particular to the fact that - in contrast to Riesling - it is an early-ripening grape variety that reaches a very high degree of ripeness and can therefore also be grown in locations that are largely unsuitable for Riesling. The wines obtained from it are aromatic and fruity, but only when the grapes are fully ripe. However, the wines sometimes lack acidity, which is why they are often blended with Müller-Thurgau.
• - Müller (-Thurgau) is a white wine that is now also called Rivaner for legal and marketing reasons. The grapes are medium to large, loose to dense berries, conical, often shouldered. The berries are medium-sized, oval, yellowish green in color and slightly scented. The berry flesh is undyed and juicy with a subtle nutmeg taste.
• - Riesling is a white grape and wine variety that is counted among the highest quality and culture-defining plants. The best Rieslings are produced in wine-growing regions with cooler climates. The variety is mainly grown in Germany, but also in numerous other wine-growing countries. Riesling wines enjoy a high reputation on international markets. Many top locations both in Germany and in other wine-growing countries are planted with Riesling.

The method according to the invention for obtaining a grape seed extract (TKE), such as e.g. As described above, it can, if desired, also be characterized in that there is an (additional) fine grinding of the grape seeds obtained by sieving to form an enriched, for example a highly enriched, grape seed extract (TKE) with an average particle size of at most about 149 microns (100 mesh ), or, for example, having an average particle size in the range of about 149 microns (100 mesh) to about 37 microns (400 mesh), or for example in the range of about 105 microns (140 mesh). In this method, the above-mentioned grape seeds, preferably the white wine grape seeds, can also be used from two or more grape varieties, that is to say from any combination of two or more of the same. The white wine grape seeds preferably used in this process are grape seeds from one or more of the grape varieties selected from the group consisting of Silvaner, Müller, Riesling and Bacchus.

A method according to the invention for obtaining a grape seed extract (TKE), e.g. previously described, can also be one that can be characterized in that it comprises a fine grinding of the grape seeds obtained by sieving and after the gentle drying described above to form an enriched, in particular a highly enriched, grape seed extract (TKE), with a average particle size ranging from about 149 microns (100 mesh) to about 44 microns (325 mesh), from about 149 microns (100 mesh) to about 53 microns (270 mesh), from about 149 microns (100 mesh) to about 63 microns (230 mesh), from about 149 microns (100 mesh) to about 74 microns (200 mesh), from about 149 microns (100 mesh) to about 88 microns (170 mesh); or that it comprises a fine grinding of the grape seeds obtained by sieving to give a fortified, in particular a highly fortified, grape seed extract (TKE) with an average particle size in the range from about 125 microns (120 mesh) to about 44 microns (325 mesh) of about 125 microns (120 mesh) to about 53 microns (270 mesh), from about 125 microns (120 mesh) to about 63 microns (230 mesh), from about 125 microns (120 mesh) to about 74 microns (200 mesh), from about 125 microns (120 mesh) to about 88 microns (170 mesh). In this method, the above-mentioned grape seeds, preferably the white wine grape seeds, can also be used from two or more grape varieties, that is to say from any combination of two or more of the same. The white wine grape seeds preferably used in this process are grape seeds from one or more of the grape varieties selected from the group consisting of Silvaner, Müller, Riesling and Bacchus.

1. (1) dreifache Siebung von Trester aus der Weinherstellung, wobei der zum Sieben zugeführte Trester an der Sieb-Zuführung mit einem Wassernebel besprengt wird, und wobei der zugeführte mit Wassernebel besprengte Trester zunächst durch ein erstes Sieb mit einer Maschenweite von etwa 8 bis 12 mm gesiebt wird; sodann das in der ersten Siebung erhaltene Siebgut durch ein zweites Sieb mit einer Maschenweite von etwa 3 bis < 8 mm gesiebt wird; und abschließend die in der zweiten Siebung erhaltenen Traubenkerne in einem dritten Sieb aufgefangen und vom als Wassernebel zugeführten Wasser abgetrennt werden;
2. (2) gefolgt von einer, möglichst gleichmäßigen, Trocknung der aus der vorhergehenden dreifachen Siebung erhaltenen Traubenkerne bei einer Temperatur von nicht mehr als etwa 60 °C, vorzugsweise bei einer Temperatur von nicht mehr als etwa 55 °C, weiter bevorzugt bei einer Temperatur von nicht mehr als etwa 50 °C; und
3. (3) nachfolgende Vermahlung der durch Siebung in (1) erhaltenen und in (2) getrockneten Traubenkerne zu einem angereicherten, insbesondere zu einem hoch-angereicherten, Traubenkernextrakt (TKE) mit einer durchschnittlichen Partikelgröße von höchstens 2000 Mikron (10 Mesh), besonders bevorzugt mit einer durchschnittlichen Partikelgröße von höchstens 1000 Mikron (18 Mesh);

und/oder bevorzugt

• nachfolgende Feinvermahlung der durch Siebung in (1) erhaltenen und in (2) getrockneten Traubenkerne zu einem angereicherten, insbesondere zu einem hoch-angereicherten, Traubenkernextrakt (TKE) (enthaltend OPC) zu einer durchschnittlichen Partikelgröße von etwa höchstens 250 Mikron (60 Mesh), gewünschtenfalls zu höchstens etwa 177 Mikron (80 Mesh), oder gewünschtenfalls zu einer durchschnittlichen Partikelgröße von höchstens etwa 149 Mikron (100 Mesh), oder gewünschtenfalls zu einer durchschnittlichen Partikelgröße im Bereich von etwa 149 Mikron (100 Mesh) bis etwa 88 Mikron (170 Mesh), oder gewünschtenfalls von etwa 105 Mikron (140 Mesh), gemahlen wird.

The method according to the invention for obtaining an enriched, in particular a highly enriched, grape seed extract (TKE), in which the additional proviso applies in each case that the Method does not involve extraction with solvents or solvent mixtures, for example, can be carried out so that it comprises at least the following three steps:

1. (1) triple sieving of pomace from wine production, whereby the pomace fed to the sieve is sprinkled with a water mist at the sieve feed, and the fed pomace, sprinkled with water mist, first through a first sieve with a mesh size of about 8 to 12 mm is sieved; then the sieved material obtained in the first sieving is sieved through a second sieve with a mesh size of about 3 to <8 mm; and finally the grape seeds obtained in the second sieving are collected in a third sieve and separated from the water supplied as a water mist;
2. (2) followed by a, as uniform as possible, drying of the grape seeds obtained from the previous triple sieving at a temperature of not more than about 60 ° C, preferably at a temperature of not more than about 55 ° C, more preferably at a temperature of no more than about 50 ° C; and
3. (3) Subsequent grinding of the grape seeds obtained by sieving in (1) and dried in (2) to give an enriched, in particular a highly enriched, grape seed extract (TKE) with an average particle size of at most 2000 microns (10 mesh), particularly preferred having an average particle size not exceeding 1000 microns (18 mesh);

and / or preferred

• Subsequent fine grinding of the grape seeds obtained by sieving in (1) and dried in (2) to give an enriched, in particular a highly enriched, grape seed extract (containing OPC) to an average particle size of about 250 microns (60 mesh), if desired, at most about 177 microns (80 mesh), or if desired, an average particle size of at most about 149 microns (100 mesh), or if desired, an average particle size in the range of about 149 microns (100 mesh) to about 88 microns (170 mesh) ), or about 105 microns (140 mesh) if desired.

In step (3), in this described method according to the invention for obtaining a grape seed extract (TKE) (containing OPC) in the case of grinding the grape seeds obtained by sieving to an enriched, in particular a highly enriched, grape seed extract (TKE) (containing OPC) the average particle size is at least> 250 microns (60 mesh). This is preferred according to the invention.

In step (3), in this described method according to the invention for obtaining a grape seed extract (TKE) (containing OPC) in the case of a fine grinding of the grape seeds obtained by sieving to give an enriched, in particular a highly enriched, grape seed extract (OPC), if desired Average particle size at least 37 microns (400 mesh), at least 44 microns (325 mesh), at least 53 microns (270 mesh), at least 63 microns (230 mesh), at least 74 microns (200 mesh), or at least 88 microns (170 mesh) , amount.

Surprisingly, it has also been shown that, in an advantageous embodiment of the invention, fine grinding to a particle size of at most 250 microns (60 mesh) can already be sufficient, surprisingly even when grape seeds are used with a lower fat content, in order to obtain the grape seed extract according to the invention (TKE) at least more than 80% (w / w) of the OPC (oligonucleotide proanthocyanidins) naturally contained in the untreated grape seeds, preferably at least more than 90% (w / w), more preferably at least more than 95% (W / w), even more preferably at least more than 96% (w / w), 97% (w / w), 98% (w / w) or 99% (w / w) / Wt.), Each based on the OPC (oligonucleotide proanthocyanidins) naturally contained in the untreated grape seeds as 100% by weight, as OPC (oligonucleotide proanthocyanidins) for the body of an animal subject, preferably a mammal, and particularly preferably ugh for a person to make physiologically available.

The average particle sizes given above, i.e. the highest, at least and ranges of the average particle sizes in microns (mesh) apply here equally to the method described above.

The term “particle size” is often also referred to as “grain size” by those skilled in the art. In the context of the present invention, the particle size is preferably determined by sieving (“sieve analysis”). Here, a set with downwardly finer sieves is placed on top of one another. The one to be analyzed The sample is poured into the top sieve and the sieve set is then clamped into a sieve machine. The machine then shakes or vibrates the sieve set for a certain period of time (dry sieving). With a high proportion of fine grains, in the prior art, sieving is also carried out with running water (wet sieving). According to the invention, however, dry sieving is preferably used to determine the particle size.

The most common method of sieve analysis is dry sieving with a sieve tower that is attached to a sieving machine. Dry sieving is a machine type of sieve analysis in which the material sample is dried in a drying oven at a temperature of 105 ° C for preparation. After cooling, the sample is weighed and then placed in the sieve system. The sieve system has smaller mesh sizes from top to bottom. When sieving with a sieve tower, several test sieves are arranged one above the other and clamped onto a sieving machine. The test sieves each consist of a sieve bottom and the sieve frame. The mesh sizes of the individual test sieves are descending from top to bottom. When performing the sieve analysis, the sample to be analyzed is placed on the coarsest test sieve and subjected to a defined movement for a specified time, which depends on the sieving method selected. The particle size distribution of the sample is then determined by weighing the residues on the individual test sieves. If there are no smaller portions than 0.063 mm, the sample can be examined with a dry sieve. Otherwise the fine parts under 0.063 mm must first be separated wet. During the sieving process, that portion remains in each sieve that has passed through the upper sieve, but has not passed through the sieve under consideration. The individual proportions are now weighed and displayed as a percentage in the form of a grain distribution (in a table and / or graphically as a grain distribution curve / sieve line).

The grain sizes determined in this way are usually given in millimeters or microns. In Anglophone countries, the unit of measurement "mesh" is also used. In the context of the present invention, particle sizes are specified in the unit of measurement “micron (mesh)”. The term “grain size” or “particle size” describes the size of individual particles in a mixture. The grain or particle size distribution has a significant influence on the material properties. The term “mesh” stands for the English word for mesh) and is a unit of “mesh fineness” in many English-speaking countries (see Anglo-American measurement system). The term “mesh” is mainly used for sieves. To calculate the mesh size from the mesh, knowledge of the wire thickness is necessary. At the same time, the “mesh” also describes the grain size (particle size) of appropriately sieved material.

The result of a grain size analysis (sieve analysis) is the grain size distribution, i.e. a frequency distribution in the form of a bar or line diagram. The percentage (weight percent) of the grains is plotted against the classified equivalent diameter (abscissa). The usual statistical parameters, such as mean value, median, percentile values, scatter or skew of the distribution, as well as the number of irregularities, can be calculated and thus the sample can be characterized with regard to its grain size or particle size. In the context of the present invention, the median value is understood as the average particle size.

1. (i) der OPC-Gehalt (Oligomere Proanthocyanidine) im gewonnenen Traubenkernextrakt (TKE) (enthaltend OPC) mindestens 10 Gew.-%, vorzugsweise mindestens 50 Gew.-%, weiter bevorzugt mindestens 70 Gew.-%, noch weiter bevorzugt mindestens 80 Gew.-%, besonders bevorzugt mindestens 90 Gew.-%, beträgt, jeweils bezogen auf die Trockenmasse des Traubenkernextrakts (TKE) als 100 Gew.-%; und/oder
2. (ii) der Fettanteil („ausgepresst Traubenkernöl benannt“) im gewonnenen Traubenkernextrakt (TKE) (enthaltend OPC) mindestens 2 Gew.-%, vorzugsweise mindestens 5 Gew.-%, weiter bevorzugt mindestens 8 Gew.-%, besonders bevorzugt mindestens 10 Gew.-%, beträgt, jeweils bezogen auf die Trockenmasse des Traubenkernextrakts (TKE) als 100 Gew.-%.

The method according to the invention for obtaining a grape seed extract (TKE) (containing OPC, oligomeric proanthocyanidins) as described above, can be characterized in that

1. (i) the OPC content (oligomeric proanthocyanidins) in the grape seed extract (TKE) obtained (containing OPC) at least 10% by weight, preferably at least 50% by weight, more preferably at least 70% by weight, even more preferably at least 80 % By weight, particularly preferably at least 90% by weight, is in each case based on the dry matter of the grape seed extract (TKE) as 100% by weight; and or
2. (ii) the fat content (called “pressed grape seed oil”) in the grape seed extract (TKE) obtained (containing OPC) is at least 2% by weight, preferably at least 5% by weight, more preferably at least 8% by weight, particularly preferably at least 10 % By weight, based in each case on the dry matter of the grape seed extract (TKE), is 100% by weight.

1. (i) der OPC-Gehalt (Oligomere Proanthocyanidine) im gewonnenen Traubenkernextrakt (TKE) (enthaltend OPC) mindestens 10 Gew.-%, vorzugsweise mindestens 50 Gew.-%, weiter bevorzugt mindestens 65 Gew.-%, noch weiter bevorzugt mindestens 70 Gew.-%, besonders bevorzugt mindestens 75 Gew.-%, beträgt, jeweils bezogen auf die Trockenmasse des Traubenkernextrakts (TKE) als 100 Gew.-%; und/oder
2. (ii) der Fettanteil im gewonnenen Traubenkernextrakt (TKE) (enthaltend OPC, Oligomere Proanthocyanidine) mindestens 2 Gew.-%, vorzugsweise mindestens 5 Gew.-%, weiter bevorzugt mindestens 7 Gew.-%, besonders bevorzugt mindestens 10 Gew.-%, beträgt, jeweils bezogen auf die Trockenmasse des Traubenkernextrakts (TKE) als 100 Gew.-%.

The invention also relates to a grape seed extract (TKE) (containing OPC, oligomeric proanthocyanidins), preferably obtained by a method according to any one of claims 1 to 7, characterized in that

1. (i) the OPC content (oligomeric proanthocyanidins) in the grape seed extract (TKE) obtained (containing OPC) at least 10% by weight, preferably at least 50% by weight, more preferably at least 65% by weight, even more preferably at least 70 % By weight, particularly preferably at least 75% by weight, in each case based on the dry matter of the grape seed extract (TKE) as 100% by weight; and or
2. (ii) the fat content in the grape seed extract (TKE) obtained (containing OPC, oligomeric proanthocyanidins) at least 2% by weight, preferably at least 5% by weight, more preferably at least 7% by weight, particularly preferably at least 10% by weight , is based in each case on the dry matter of the grape seed extract (TKE) as 100% by weight.

The invention also relates to a composition containing a grape seed extract (TKE) according to the invention (containing OPC, oligomeric proanthocyanidins), as described above, preferably obtained by one of the previously described methods according to the invention, which can be characterized in that the grape seed extract (TKE) (containing OPC ) in a mixture with one or more substances selected from the group consisting of damiana, tadalfil, glycerine, emulsifier, pharmaceutical carriers, pharmaceutical excipients, rose water, wheat germ oil and evening primrose oil.

Composition containing a grape seed extract (TKE) according to the invention (containing OPC, oligomeric proanthocyanidins) per se, and optionally also an aforementioned composition according to the invention containing a grape seed extract (TKE) according to the invention (containing OPC, oligomeric proanthocyanidins) in a mixture with one or more of the aforementioned substances, may, if desired, comprise further pharmaceutical and / or food-technically acceptable or physiologically acceptable carriers, auxiliaries, binders, emulsifiers, lubricants, fillers, disintegrants, etc.

The above compositions can be packaged as bulk goods, in cans, in bags, in sachets, in capsules, as powder or granules, or compressed into tablets. Capsules are preferred, in particular as a daily dose for daily single intake, preferably vegetarian, more preferably vegan capsules.

In a composition according to the invention, containing a mixture of grape seed extract (TKE) (containing OPC, oligomeric proanthocyanidins) with one or more of the substances mentioned above, the respective amount of grape seed extract (TKE) according to the invention (containing OPC, oligomeric proanthocyanidins) and the one or more of the substances mentioned above vary within a wide range. As a rule, a mixture according to the invention will contain the grape seed extract (TKE) (containing OPC, oligomeric proanthocyanidins) in an amount of at least 10% by weight, based on the total amount of the composition as 100% by weight. Examples of such compositions according to the invention are compositions containing a mixture of 10% by weight to 90% by weight grape seed extract (TKE) (containing OPC, oligomeric proanthocyanidins) and 10% by weight to 90% by weight of one or more of the the aforementioned substances, if desired containing further other pharmaceutical and / or food-technically safe or physiologically acceptable carriers, auxiliaries, binders, emulsifiers, lubricants, fillers, disintegrants in an amount of at most 30% by weight, preferably at most 25% by weight, more preferably at most 20% by weight, even more preferably at most 15% by weight, in each case based on the total amount of the composition as 100% by weight.

In further preferred compositions according to the invention, the further pharmaceutical and / or food-technically acceptable or physiologically acceptable carriers, auxiliaries, binders, lubricants, fillers, disintegrants, etc., which may be present, are at most in an amount of at most 10% by weight, preferably at most 5% by weight. -%, each based on the total amount of the composition as 100% by weight. Very particularly preferred compositions according to the invention, in particular those containing only grape seed extract (TKE) (containing OPC, oligomeric proanthocyanidins), but none of the other aforementioned substances, are essentially free of any additives, in particular also essentially free of other pharmaceutical and / or food-safe substances or physiologically acceptable carriers, auxiliaries, binders, emulsifiers, lubricants, fillers, disintegrants, etc.

In a particular composition according to the invention is a mixture of grape seed extract (TKE) (containing OPC, oligomeric proanthocyanidins) in an amount of 10 wt .-% to 90 wt .-% and at least one of the substances Damiana and / or Tadalfil in an amount of 10 % By weight to 90% by weight, each based on the total amount of the composition as 100% by weight. The amounts of grape seed extract (TKE) (containing OPC, oligomeric proanthocyanidins) and the amounts of the at least one of the substances damiana and / or tadalfil can vary as desired in the specified percentage weight ranges. For example, the compositions according to the invention can be a mixture that contains grape seed extract (TKE) (containing OPC, oligomeric proanthocyanidins) in an amount of 50 ± 5% by weight and at least one of the substances damiana and / or tadalfil in an amount of 50 ± 5% by weight .-%, each based on the total amount of the composition as 100% by weight.

According to the invention, the proportion of damiana, or also of tadalfil, can be increased variably depending on the type and intensity of the complaints or ailments occurring in an animal subject, preferably a mammal, more preferably a human. A small proportion of OPC (eg 10% by weight) in Damiana, for example, already strengthens the aphrodisiac effect of Damiana.

The invention also relates to a composition containing a grape seed extract (TKE) according to the invention (containing OPC, oligomeric proanthocyanidins), as described above, preferably obtained by one of the previously described methods according to the invention, which can be characterized in that the composition is a food or a food additive , a dietary supplement, an animal feed additive, a cosmetic or a cosmetic additive, or an aphrodisiac.

The following examples are intended to explain the invention further without, however, restricting it to the examples.

Examples

Manufacturing processes and applications for particularly effective grape seed extract (TKE), preferably from German grapes.

Example 1 (introduction)

• - Proanthocyanidin;
• - Procyanidin;
• - procyanidolische Oligomere (PCO);
• - Leucoanthocyanine

Explanation of terms: In everyday language, grape seed extract is usually only called oligomeric proanthocyanidins (OPC) and also includes the following other synonyms:

• - proanthocyanidin;
• - procyanidin;
• - procyanidolic oligomers (PCO);
• - Leucoanthocyanines

These polyphenol-based basic structures, called oligomeric proanthocyanidins, are, in addition to tannin, lignin, falvenoids such as quercetin and especially epigallocatechin-3-gallate, a catechin also a component in green tea, cinnamic acid derivatives such as 3,4-dihydroxy-cinnamic acid (also in coffee) , Coumarins and stilpenoids such as resveratrol are present in some plants and fruits, but are especially enriched in peel and especially enriched in the seeds of grapes. The other non-OPC-named ingredients in grape seeds, especially epigallocatechin-3-gallate, are also used for cancer treatment ( WO 2012171114 ) known. The effects of resveratol against cancer cells are also shown in chemoprevention in experimental animals in the Annals of the New York Academy of Sciences. Volume 1215, January 2011, pp. 60-71 (doi: 10.1111 / j.1749-6632.2010.05873.x). Resveratol itself also has preventive properties against Alzheimer's (K. Ono, MM Condron, L. Ho et al: Effects of grape seed-derived polyphenol on amyloid beta-protein self-assembly and cytotoxicity. In: Journal of Biological Chemistry 283, 2008, p. 32176-32187. (1, doi: 10.1111 / j.1749-6632.2010.05873.x), attributed to atherosclerosis and arthritis.

In English, OPC compounds are also called "condensed tannins", biochemically OPCs are classified as flavonoids - i.e. secondary plant substances. The basic structure of the flavonoids (and thus the OPC) is made up of two phenyl rings and one heterocyclic ring. As a rule, the OPCs are di- or trimers of the catechins. The latter are chroman derivatives and come in several isoforms. The basic building block of the compounds is flavan-3-ol - a flavan derivative. This flavan-3-ol unit acts as a monomer in the formation of OPCs, from which di- and trimers (as well as other higher-quality oligomers and polymers) are formed via polymerization. The flavan-3-ol has two CAS numbers for the same compound.

Examples of so-called OPCs are the following derivatives: Oligomer name Monomer name CAS no. Proguibourtinidin Guibourtinidol CAS: 251909-54-3 Prorobinetidine Robinetin idol CAS: 528-56-3 Propelargonidine Afzelechin CAS: 2545-00-8 Prodelphinidin Gallocatechol CAS: 970-73-0

The isolation and description of the OPC goes back to the French chemist Jacques Masquelier, who published his work in 1948. The discovery laid the foundation for further research into the positive effects of botanicals. OPC can now be obtained industrially. On the basis of Masquelier's work, herbal medicinal products were subsequently developed, which under obtained from pine trees and grapes. In recent years, against the background of increasingly intensive research, scientists have discovered more and more new proanthocyanidins in plants. For example, researchers from Germany have succeeded for the first time in isolating various proanthocyanidin compounds - for example procyanidin B3 and B4 - from Myrothamnus flabellifolia, which is native to Africa. Research groups are increasingly concerned with the positive effects of OPC on various diseases such as: kidney disease; HIV infections; Insulin resistance / diabetes; cognitive impairments, etc.

Against the background of the antimicrobial / anti-inflammatory effects, research into proanthocyanidins also plays a role in ophthalmology, the treatment of bacterial infectious diseases or inflammations (such as pancreatitis). In the meantime, studies are even underway looking for a positive effect of OPC in connection with intoxication. In recent years, research groups have found OPC in different concentrations, among others in: cranberries; Apples; Ryegrass; Festuca arundinacea; Cocoa (Theobroma cacao); Hazelnuts; Rice.

Example 2 (invention)

The oligomeric proanthocyanidins (OPC) have so far been preferred with solvent mixtures (alcohols, ethyl acetate) as in Faming Zhuanli Shenqing (2013), CN 103265520 and WO 2013100003 described from pomace (waste product of winemaking after pressing) especially extracted from grape seeds and their powder. The fat portion is NOT pressed out as grape seed oil beforehand. The method according to the invention is particularly characterized by the fact that no extractant is added, because if, for example, ethanol is used alone or together with ethyl acetate for the extraction, the named fat content remains largely in the residue (not in the extract) and the positive biological effect of the grape seed extract with or without reduced fat content is greatly reduced. Even with completely untreated red wine grape seeds, there is an increased OPC content optionally in the pure seeds, since red wine is mostly fermented, tempered, or even completely fermented in the mash (in order to obtain very dark red wine) due to the alcohol produced during fermentation the OPC in the remaining seeds is greatly reduced (enriched in wine). Furthermore, when using a chemical extraction process with solvents such as ethanol, ethyl acetate or even acetone, the other compounds that also have a positive effect in addition to OPC are left behind. The positive effect according to the invention of the fat percentage with regard to the absorption of OPCs in the body was already mentioned at the beginning.

Stage 1: Sieving (fine sieving)

These are separated with a vibrating sieve machine (sieve diameter 1.50 m) containing 3 sieves at the feed point with a water nozzle sprinkled with water mist.

The top sieve is 10 mm and is used to separate the stems that are still present, the second sieve is 5 mm and isolates the grape seeds. The third sieve allows the water supplied with the water nozzle to be drained. The water removes dust and small particles containing impurities and makes the pomace pliable before it is sieved. Without a water nozzle, there is no clean separation of shells and kernels, only contaminated kernels are obtained, and the yield of isolated kernels is reduced by up to 50% depending on the vintage and the loading of the sieving machine. With this device, 1–3 m ^{3 of} pomace that has already been destemmed to 90% (without stalks) is sieved per hour. If the marc is not destemmed, the output per hour is around 1 m ³ .

shows an example of a picture of a screening machine.

Stage 2: The drying

Very even drying no higher than 50 ° C is very advantageous. The water content is reduced by up to 50% of the original weight after the wine has been pressed. Too high a temperature, as it is mostly used by Chinese suppliers for the production of grape seed flour for the extraction and then again for the spray drying, considerably reduces the bioavailable phenol / OPC content and only leads to the ingestion due to the poorly soluble, now higher-chain flavenol derivatives to a greatly reduced effectiveness. Either a drying cabinet can be used for drying or, much more gently, fluidized bed drying. The use of fluidized bed drying enables shorter oligomer chains, more uniform drying and, associated with this, easier grinding after drying of the cores.

shows an example of a fluidized bed dryer.

Stage 3: The grinding

It is known that higher oligomers are broken down by the intestinal flora. However, this takes time. So the grain size together with the fat percentage is very important for good absorption in the body. The fluidized bed dryer enables grinding with a universal turbo mill to 105 microns (140 mesh) without sticking and only when the material to be ground is slightly heated (max. 40 ° C). The OPC of this particle size can then be absorbed particularly well by the body together with the fat content.

Analysis of the grape seed extract obtained according to the invention:

The analyzes were carried out at the Technical University of Braunschweig as HPLC analyzes against standard reference compounds. The publication Kuhnert, S .; Lehmann, L .; Winterthaler, P .; "Rapid characterization of grape seed extracts by a novel HPLC method on a diol stationary phase", Journal of functional Foods, 2015, 15, pp. 225-232 , referenced.

According to the above inventive method, e.g. a grape seed extract according to the invention with the following composition was obtained: The OPC content (measured by HPLC analysis) was about 79.96% by weight, based on the total grape seed extract obtained (TKE) as 100% by weight. In addition to the above-mentioned OPC, the grape seed extract (TKE) obtained, also based on the total grape seed extract (TKE) obtained as 100% by weight, contained grape seed oil and a resulting fat content of about 14.34% by weight (measured by HPLC analysis ). The difference to 100% by weight was water, which ensured that only shorter oligomers were extracted from OPC.

The fat content mentioned in the grape seed extract (TKE) according to the invention, resulting from the grape seed oil contained therein, had the following composition in g / 100 g fat: about 12.91 g saturated fatty acids; about 17.38 g of monounsaturated fatty acids; about 68.35 grams of polyunsaturated fatty acids; a total of 98.64 g of saturated, monounsaturated and polyunsaturated fatty acids per 100 g of fat.

In each case based on the entire grape seed oil-containing and OPC-containing grape seed extract (TKE) obtained here according to the invention as 100% by weight, this grape seed extract (TKE) according to the invention thus contained fat of the following composition and the following amounts: about 1.85 g (1.85 g) % By weight) saturated fatty acids; about 2.49 g (2.49% by weight) of monounsaturated fatty acids; about 9.80 g (9.80 wt%) polyunsaturated fatty acids; in total 14.15 g (14.15% by weight) of saturated, monounsaturated and polyunsaturated fatty acids.

The above grape seed extract (TKE) obtained according to the invention was used in the following application examples 3 to 5 and is referred to there as “OPC” in accordance with common usage.

Example 3 (invention): New OPC mixtures of grape seed extract according to the invention (TKE) with damiana

Another component of this invention is the use of OPC from grape seed extract according to the invention (TKE) as a mixture with Damiana leaves ground to the same particle size. It is known that OPC has a blood vessel dilatation effect, this is achieved especially by mixing it with Damiana extract, this effect is accompanied by a strong aphrodisiac effect which was already recognized and used by the Mayans. Particularly in Asian countries, these effects are assigned special properties and can potentially replace ivory from rhinos and elephant tusks and other animals. Damiana is a popular medicinal herb, especially in Mexico. There it is used under the names Misibcoc or Chac-Mixib to cure colds, infectious diseases or diseases of the blood vessels. For this, a tea is made from dried leaves or a liqueur is flavored with the leaves. The dried leaves can also be found in markets in Mexico and Guatemala. Smaller quantities are also available in the United States and Europe, where damiana is relatively unknown as a medicinal plant. The combination of Damiana with OPC is completely unknown. A positive effect on women during menstruation has also been described.

• - Estrada-Reyes R, Ortiz-Löpez P, Gutierrez-Ortiz J, Martinez-Mota L: Turnera diffusa Wild (Turneraceae) recovers sexual behavior in sexually exhausted males. In: J Ethnopharmacol. 25. Juni 2009, Band 123, Nr. 3, S. 423-429 , PMID 19501274.
• - Estrada-Reyes R, Carro-Juarez M, Martinez-Mota L: Pro-sexual effects of Turnera diffusa Wild (Turneraceae) in male rats involves the nitric oxide pathway. In: J Ethnopharmacol. 7. März 2013, Band 146, Nr. 1, S. 164-172 , PMID 23298455.
• - In Thomas P. Lowry: Damiana. In: Journal of Psychoactive Drugs. Band 16, Nr. 3, 1984, S. 267-268 ist die Abmilderung von Menstruationsbeschwerden beschrieben.
• - In Patentveröffentlichungen ist Damiana in Faming Zhuanli Shenqing (2013), CN 103356751 A Oct 23, 2013, Titel (Machine Translation): „Compound extract for promoting sexual function and male sexual organ growth“ und WO 2015027201 , Titel: „Methods and compositions for enhancing female sexual wellness“ beschrieben.

The above mentioned properties are described in the following scientific articles:

• - Estrada-Reyes R, Ortiz-Löpez P, Gutierrez-Ortiz J, Martinez-Mota L: Turnera diffusa Wild (Turneraceae) recovers sexual behavior in sexually exhausted males. In: J Ethnopharmacol. June 25, 2009, Volume 123, No. 3, pp. 423-429 , PMID 19501274.
• - Estrada-Reyes R, Carro-Juarez M, Martinez-Mota L: Pro-sexual effects of Turnera diffusa Wild (Turneraceae) in male rats involves the nitric oxide pathway. In: J Ethnopharmacol. March 7, 2013, Volume 146, No. 1, pp. 164-172 , PMID 23298455.
• - In Thomas P. Lowry: Damiana. In: Journal of Psychoactive Drugs. Volume 16, No. 3, 1984, pp. 267-268 the alleviation of menstrual cramps is described.
• - In patent publications, Damiana is in Faming Zhuanli Shenqing (2013), CN 103356751 A Oct 23, 2013, Title (Machine Translation): "Compound extract for promoting sexual function and male sexual organ growth" and WO 2015027201 , Title: "Methods and compositions for enhancing female sexual wellness".

Example 4 (invention): New OPC mixtures of grape seed extract according to the invention (TKE) with tadalafil (Calais)

Tadalafil (now generic) was developed by Lilly around the turn of the millennium and is a cyclic guanosine phosphate (cGMP) specific phosphorodiesterase 5 (PDE5) inhibitor and is being discussed by Viaga as a new, better alternative. If tadalafil is taken according to the invention as a mixture with OPC from grape seed extract (TKE) according to the invention, the effectiveness is improved. Mixtures of OPC from grape seed extract according to the invention (TKE) with Damiana and Tadalafil are also according to the invention.

Example 5 (Invention): OPC from grape seed extract (TKE) according to the invention as a face and full body mask

It is known that in the early days of viticulture in Europe, before pressing techniques were developed, the grapes were trodden with the feet after the harvest. Even at that time, people were amazed at the good, healthy skin on the workers' feet. According to the invention it has now been found that very finely ground grape seeds with 140 mesh (105 microns) emulsified in glycerine or other carrier substances using the above-mentioned process are suitable as a skin care product. Information on glycerin can be found at http://www.biokontakte.com/artikel/kosmetik-pfleqe/q lvcerin-in-kosmetik-part-1-definition-task-and-effect. The general use of OPC in skin care products and cosmetics is already known, for example from Henkel in EP 2 742 927 , JP 2009161440 , JP 2005029486 and WO 9966881 (OPC from extract from bark) and older works such as by Ariga, Toshiaki; and Yuasa, Katsumi in Fragrance Journal (1994), 22 (7), 52-6 and WO 9324106 described.
http://www.institut-hautnah-kosmetik.de/anqebot/opc-creme-plus-mit-traubenkernextrakt/
https: //austriaforum.orq/af/Videos/Unternehmen/Vinoble Cosmetics watch how cosmetics are made from grape seeds

1. a) OPC-Gesichtswasser:
   • Zutaten:
     • 50 mg OPC (in Kapselform) aus erfindungsgemäßem Traubenkernextrakt (TKE)
     • 50 mg Rosenwasser (in der Apotheke bzw. Drogeriemarkt erhältlich!) Watte
   • Anleitung:
     • Aufschneiden zunächst einer 50 mg OPC-Kapsel und auflösen des darin enthaltenen Pulvers in 50 mg Rosenwasser.
     • Kurz schütteln, einen Wattebausch darin tränken und damit das Gesicht reinigen!
2. b) OPC-Körperlotion:
   • Zutaten:
     • 50 mg OPC (in Kapselform) aus erfindungsgemäßem Traubenkernextrakt (TKE)
     • 50 mg Weizenkeim-Nachtkerzenöl-Mischung (in der Apotheke bzw. Drogeriemarkt erhältlich, Mischungsverhältnis 1:1!)
   • Anleitung:
     • Aufschneiden zunächst einer 50 mg OPC-Kapsel aus erfindungsgemäßem Traubenkernextrakt (TKE)
     • Vermengen des darin enthaltenen Pulvers mit 50 mg Weizenkeim-Nachtkerzenöl-Mischung (je 25 mg)
     • Gleichmäßiges Einreiben nach dem Waschen bzw. Duschen des Gesichts bzw. eines Teils oder des ganzen Körpers mit der OPC-Lotion!

Further examples from cosmetics:

1. a) OPC facial toner:
   • Ingredients:
     • 50 mg OPC (in capsule form) from grape seed extract according to the invention (TKE)
     • 50 mg rose water (available in pharmacies or drugstores!) Cotton wool
   • Manual:
     • First cut open a 50 mg OPC capsule and dissolve the powder it contains in 50 mg rose water.
     • Shake briefly, soak a cotton ball in it and use it to clean your face!
2. b) OPC body lotion:
   • Ingredients:
     • 50 mg OPC (in capsule form) from grape seed extract according to the invention (TKE)
     • 50 mg wheat germ and evening primrose oil mixture (available in pharmacies or drugstores, mixing ratio 1: 1!)
   • Manual:
     • First cut open a 50 mg OPC capsule made from grape seed extract according to the invention (TKE)
     • Mix the contained powder with 50 mg wheat germ-evening primrose oil mixture (25 mg each)
     • Rub in the OPC lotion evenly after washing or showering the face or part or the whole body!

QUOTES INCLUDED IN THE DESCRIPTION

This list of the documents listed by the applicant was generated automatically and is included solely for the better information of the reader. The list is not part of the German patent or utility model application. The DPMA assumes no liability for any errors or omissions.

Patent literature cited

• US 8383413 B2 [0046, 0047]
• WO 2012171114 [0087]
• CN 103265520 [0092]
• WO 2013100003 [0092]
• CN 103356751 A [0105]
• WO 2015027201 [0105]
• EP 2742927 [0107]
• JP 2009161440 [0107]
• JP 2005029486 [0107]
• WO 9966881 [0107]
• WO 9324106 [0107]

Non-patent literature cited

• Kuhnert, S .; Lehmann, L .; Winterthaler, P .; "Rapid characterization of grape seed extracts by a novel HPLC method on a diol stationary phase", Journal of functional Foods, 2015, 15, pp. 225-232 [0099]
• Estrada-Reyes R, Ortiz-Löpez P, Gutierrez-Ortiz J, Martinez-Mota L: Turnera diffusa Wild (Turneraceae) recovers sexual behavior in sexually exhausted males. In: J Ethnopharmacol. June 25, 2009, Volume 123, No. 3, pp. 423-429 [0105]
• Estrada-Reyes R, Carro-Juarez M, Martinez-Mota L: Pro-sexual effects of Turnera diffusa Wild (Turneraceae) in male rats involves the nitric oxide pathway. In: J Ethnopharmacol. March 7, 2013, Volume 146, No. 1, pp. 164-172 [0105]
• Thomas P. Lowry: Damiana. In: Journal of Psychoactive Drugs. Volume 16, No. 3, 1984, pp. 267-268 [0105]

Claims (10)

Process for obtaining a grape seed extract (TKE) (containing OPC, oligomeric proanthocyanidins), characterized in that it involves the extraction of grape seeds by sieving pomace from winemaking and grinding the grape seeds obtained by sieving into an enriched, preferably a highly enriched one , Grape seed extract (TKE) (containing OPC) with an average particle size of at most 2000 microns (10 mesh), in particular with an average particle size of at most 1000 microns (18 mesh), and / or preferably by fine grinding to an enriched, preferably to one highly enriched grape seed extract (TKE) (containing OPC) with an average particle size of about at most 250 microns (60 mesh); with the additional proviso that the process does not include extraction with solvents or solvent mixtures. Process for obtaining a grape seed extract (TKE) (containing OPC) according to Claim 1 , characterized in that in the process the pomace fed to the sieve is sprinkled with a water mist at the sieve feed, and optionally the (moist) grape seeds obtained by sieving at a temperature of not more than about 60 ° C, preferably at a temperature of not more than about 55 ° C, more preferably at a temperature of not more than about 50 ° C, (gently and as evenly as possible) to obtain an enriched, preferably a highly enriched, grape seed extract (TKE) (containing OPC). Process for obtaining a grape seed extract (TKE) (containing OPC) according to one of the Claims 1 or 2 , characterized in that for obtaining an enriched, in particular a highly enriched, grape seed extract (TKE) (containing OPC), those grape seeds are used which comprise or consist of white wine grape seeds. Process for obtaining a grape seed extract (TKE) (containing OPC) according to one of the preceding claims, characterized in that the white wine grape seeds from one or more of the grape varieties are selected from the group consisting of Silvaner, Müller, Riesling, Bacchus, Pinot Blanc, Pinot Gris, Scheurebe , Rislaner, Morio Muscat, Chardonnay, Traminer, Kerner and Sauvignon Blanc; preferably one or more of the grape varieties selected from the group consisting of Silvaner, Müller, Riesling and Bacchus. Method for obtaining a grape seed extract (TKE) (containing OPC) according to one of the preceding claims, characterized in that it involves fine grinding of the grape seeds obtained by sieving to an enriched, preferably a highly enriched, grape seed extract (TKE) (containing OPC) an average particle size of at most about 149 microns (100 mesh), more preferably having an average particle size in the range of about 149 microns (100 mesh) to about 37 microns (400 mesh), more preferably in the range of about 105 microns (140 mesh). Process for obtaining a fortified, preferably a highly fortified, grape seed extract (TKE) (containing OPC), characterized in that it comprises at least the following three steps: (1) triple sieving of pomace from winemaking, with the pomace supplied for sieving is sprinkled with a water mist at the sieve feed, and the pomace sprinkled with water mist is first sieved through a first sieve with a mesh size of about 8 to 12 mm; then the sieved material obtained in the first sieving is sieved through a second sieve with a mesh size of about 3 to <8 mm; and finally the grape seeds obtained in the second sieving are collected in a third sieve and separated from the water supplied as a water mist; (2) followed by a (as uniform as possible) drying of the grape seeds obtained from the previous triple sieving at a temperature of not more than about 60 ° C, preferably at a temperature of not more than about 55 ° C, more preferably at a temperature of no more than about 50 ° C; and (3) subsequent grinding of the grape seeds obtained by sieving in (1) and dried in (2) to give a fortified, preferably a highly fortified, grape seed extract (containing OPC) with an average particle size of at most 2000 microns (10 Mesh), more preferably having an average particle size of at most 1000 microns (18 mesh); and / or preferably subsequent fine grinding of the grape seeds obtained by sieving in (1) and dried in (2) to give an enriched, preferably a highly enriched, grape seed extract (containing OPC) to an average particle size of about 250 microns ( 60 mesh), more preferably at most about 177 microns (80 mesh), preferably at an average particle size of at most about 149 microns (100 mesh), more preferably at an average particle size in the range of about 149 microns (100 mesh) to about 88 microns (170 mesh), more preferably about 105 microns (140 mesh). Process for obtaining a grape seed extract (TKE) (containing OPC) according to one of the preceding claims, characterized in that (i) the OPC content (oligomeric proanthocyanidins) in the grape seed extract (TKE) obtained (containing OPC) is at least 50% by weight, preferably at least 60% by weight, more preferably at least 65% by weight, even more preferably at least 70% by weight, particularly preferably at least 75% by weight, based in each case on the dry matter of the grape seed extract (TKE) (containing OPC) as 100 wt%; and / or (ii) the fat content (“named pressed grape seed oil”) in the grape seed extract (TKE) obtained (containing OPC) at least 2% by weight, preferably at least 5% by weight, more preferably at least 7% by weight, particularly preferably at least 10% by weight, based in each case on the dry matter of the grape seed extract (TKE) (containing OPC) as 100% by weight. Grape seed extract (TKE) (containing OPC, oligomeric proanthocyanidins), preferably obtained by a method according to one of the Claims 1 to 7th , characterized in that (i) the OPC content (oligomeric proanthocyanidins) in the grape seed extract (TKE) obtained (containing OPC) at least 50% by weight, preferably at least 60% by weight, more preferably at least 65% by weight, even more preferably at least 70% by weight, particularly preferably at least 75% by weight, in each case based on the dry matter of the grape seed extract (TKE) (containing OPC) as 100% by weight; and / or (ii) the fat content in the grape seed extract (TKE) obtained (containing OPC, oligomeric proanthocyanidins) at least 2% by weight, preferably at least 5% by weight, more preferably at least 7% by weight, particularly preferably at least 10% by weight .-%, based in each case on the dry matter of the grape seed extract (TKE) (containing OPC) as 100% by weight. Composition containing a grape seed extract (TKE) (containing OPC, oligomeric proanthocyanidins) according to Claim 8 , preferably obtained by a process according to one of the Claims 1 to 7th , characterized in that the grape seed extract (TKE) (containing OPC) is present as a mixture with one or more substances selected from the group consisting of damiana, tadalfil, glycerin, emulsifier, pharmaceutical carriers, pharmaceutical excipients, rose water, wheat germ oil and evening primrose oil. Composition containing a grape seed extract (TKE) (containing OPC, oligomeric proanthocyanidins) according to Claim 8 or after Claim 9 , preferably obtained by a process according to one of the Claims 1 to 7th , characterized in that the composition is a food or a food additive, a food supplement, an animal feed additive, a cosmetic or a cosmetic additive, or an aphrodisiac.

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