DE102016224865B8 - Biopolare Elektrode zur impedimetrischen Untersuchung und Manipulation lebender Zellen in vitro - Google Patents

Biopolare Elektrode zur impedimetrischen Untersuchung und Manipulation lebender Zellen in vitro Download PDF

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Publication number
DE102016224865B8
DE102016224865B8 DE102016224865.0A DE102016224865A DE102016224865B8 DE 102016224865 B8 DE102016224865 B8 DE 102016224865B8 DE 102016224865 A DE102016224865 A DE 102016224865A DE 102016224865 B8 DE102016224865 B8 DE 102016224865B8
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Prior art keywords
impedimetric
vitro
manipulation
examination
living cells
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DE102016224865.0A
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DE102016224865B3 (de
Inventor
Christian Götz
Joachim Wegener
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Fraunhofer Gesellschaft zur Forderung der Angewandten Forschung eV
Universitaet Regensburg
Original Assignee
Fraunhofer Gesellschaft zur Forderung der Angewandten Forschung eV
Universitaet Regensburg
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Priority to DE102016224865.0A priority Critical patent/DE102016224865B8/de
Priority to US15/839,379 priority patent/US10895546B2/en
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    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N27/00—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means
    • G01N27/02—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating impedance
    • G01N27/04—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating impedance by investigating resistance
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M1/00—Apparatus for enzymology or microbiology
    • C12M1/34—Measuring or testing with condition measuring or sensing means, e.g. colony counters
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N27/00—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means
    • G01N27/26—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating electrochemical variables; by using electrolysis or electrophoresis
    • G01N27/28—Electrolytic cell components
    • G01N27/30—Electrodes, e.g. test electrodes; Half-cells
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N27/00—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means
    • G01N27/26—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating electrochemical variables; by using electrolysis or electrophoresis
    • G01N27/28—Electrolytic cell components
    • G01N27/30—Electrodes, e.g. test electrodes; Half-cells
    • G01N27/327—Biochemical electrodes, e.g. electrical or mechanical details for in vitro measurements
    • G01N27/3271—Amperometric enzyme electrodes for analytes in body fluids, e.g. glucose in blood
    • G01N27/3273—Devices therefor, e.g. test element readers, circuitry
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/483—Physical analysis of biological material
    • G01N33/4833—Physical analysis of biological material of solid biological material, e.g. tissue samples, cell cultures
    • G01N33/4836—Physical analysis of biological material of solid biological material, e.g. tissue samples, cell cultures using multielectrode arrays
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/5005—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells
    • H—ELECTRICITY
    • H01—ELECTRIC ELEMENTS
    • H01B—CABLES; CONDUCTORS; INSULATORS; SELECTION OF MATERIALS FOR THEIR CONDUCTIVE, INSULATING OR DIELECTRIC PROPERTIES
    • H01B5/00—Non-insulated conductors or conductive bodies characterised by their form
    • H01B5/14—Non-insulated conductors or conductive bodies characterised by their form comprising conductive layers or films on insulating-supports
    • H—ELECTRICITY
    • H01—ELECTRIC ELEMENTS
    • H01B—CABLES; CONDUCTORS; INSULATORS; SELECTION OF MATERIALS FOR THEIR CONDUCTIVE, INSULATING OR DIELECTRIC PROPERTIES
    • H01B1/00—Conductors or conductive bodies characterised by the conductive materials; Selection of materials as conductors
    • H01B1/06—Conductors or conductive bodies characterised by the conductive materials; Selection of materials as conductors mainly consisting of other non-metallic substances
    • H01B1/08—Conductors or conductive bodies characterised by the conductive materials; Selection of materials as conductors mainly consisting of other non-metallic substances oxides
    • H—ELECTRICITY
    • H01—ELECTRIC ELEMENTS
    • H01B—CABLES; CONDUCTORS; INSULATORS; SELECTION OF MATERIALS FOR THEIR CONDUCTIVE, INSULATING OR DIELECTRIC PROPERTIES
    • H01B1/00—Conductors or conductive bodies characterised by the conductive materials; Selection of materials as conductors
    • H01B1/06—Conductors or conductive bodies characterised by the conductive materials; Selection of materials as conductors mainly consisting of other non-metallic substances
    • H01B1/12—Conductors or conductive bodies characterised by the conductive materials; Selection of materials as conductors mainly consisting of other non-metallic substances organic substances
    • H01B1/124—Intrinsically conductive polymers
    • H—ELECTRICITY
    • H01—ELECTRIC ELEMENTS
    • H01B—CABLES; CONDUCTORS; INSULATORS; SELECTION OF MATERIALS FOR THEIR CONDUCTIVE, INSULATING OR DIELECTRIC PROPERTIES
    • H01B1/00—Conductors or conductive bodies characterised by the conductive materials; Selection of materials as conductors
    • H01B1/06—Conductors or conductive bodies characterised by the conductive materials; Selection of materials as conductors mainly consisting of other non-metallic substances
    • H01B1/12—Conductors or conductive bodies characterised by the conductive materials; Selection of materials as conductors mainly consisting of other non-metallic substances organic substances
    • H01B1/124—Intrinsically conductive polymers
    • H01B1/127—Intrinsically conductive polymers comprising five-membered aromatic rings in the main chain, e.g. polypyrroles, polythiophenes
    • H—ELECTRICITY
    • H01—ELECTRIC ELEMENTS
    • H01B—CABLES; CONDUCTORS; INSULATORS; SELECTION OF MATERIALS FOR THEIR CONDUCTIVE, INSULATING OR DIELECTRIC PROPERTIES
    • H01B1/00—Conductors or conductive bodies characterised by the conductive materials; Selection of materials as conductors
    • H01B1/06—Conductors or conductive bodies characterised by the conductive materials; Selection of materials as conductors mainly consisting of other non-metallic substances
    • H01B1/12—Conductors or conductive bodies characterised by the conductive materials; Selection of materials as conductors mainly consisting of other non-metallic substances organic substances
    • H01B1/124—Intrinsically conductive polymers
    • H01B1/128—Intrinsically conductive polymers comprising six-membered aromatic rings in the main chain, e.g. polyanilines, polyphenylenes

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Physics & Mathematics (AREA)
  • Biomedical Technology (AREA)
  • Immunology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Analytical Chemistry (AREA)
  • General Physics & Mathematics (AREA)
  • Molecular Biology (AREA)
  • Pathology (AREA)
  • Hematology (AREA)
  • Urology & Nephrology (AREA)
  • Medicinal Chemistry (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Electrochemistry (AREA)
  • Food Science & Technology (AREA)
  • Biotechnology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Microbiology (AREA)
  • Biophysics (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Organic Chemistry (AREA)
  • Optics & Photonics (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Cell Biology (AREA)
  • General Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Sustainable Development (AREA)
  • Investigating Or Analyzing Materials By The Use Of Electric Means (AREA)
DE102016224865.0A 2016-12-13 2016-12-13 Biopolare Elektrode zur impedimetrischen Untersuchung und Manipulation lebender Zellen in vitro Active DE102016224865B8 (de)

Priority Applications (2)

Application Number Priority Date Filing Date Title
DE102016224865.0A DE102016224865B8 (de) 2016-12-13 2016-12-13 Biopolare Elektrode zur impedimetrischen Untersuchung und Manipulation lebender Zellen in vitro
US15/839,379 US10895546B2 (en) 2016-12-13 2017-12-12 Bipolar electrode for the impedimetric examination and manipulation of living cells in vitro

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
DE102016224865.0A DE102016224865B8 (de) 2016-12-13 2016-12-13 Biopolare Elektrode zur impedimetrischen Untersuchung und Manipulation lebender Zellen in vitro

Publications (2)

Publication Number Publication Date
DE102016224865B3 DE102016224865B3 (de) 2018-05-03
DE102016224865B8 true DE102016224865B8 (de) 2018-07-26

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US (1) US10895546B2 (de)
DE (1) DE102016224865B8 (de)

Families Citing this family (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN110426439B (zh) * 2019-07-03 2020-10-27 淮阴工学院 无线光电化学间接分析检测装置及其制作方法
DE102020209915B4 (de) 2020-08-05 2022-08-18 Universität Rostock In vitro/ex vivo Modell zur Schadstoff- und Medikamententestung
RU202093U1 (ru) * 2020-11-24 2021-02-01 Общество с ограниченной ответственностью Научно-технический центр «БиоКлиникум» (ООО НТЦ «БиоКлиникум») Устройство для измерения спектра импеданса биологических структур
CN114264708A (zh) * 2021-12-22 2022-04-01 合肥工业大学 一种用于化学沉积中电势影响快速分析的方法

Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2013012498A1 (en) 2011-06-14 2013-01-24 Hemoshear, Llc Electric cell- substrate impedance sensing (ecis) of biological samples in shear stress flow
WO2016139566A1 (en) 2015-03-03 2016-09-09 Abdolahad Mohammad Electrical cell-substrate impedance sensor (ecis)

Family Cites Families (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4194670A (en) * 1977-08-24 1980-03-25 Chlorine Engineers Corp., Ltd. Method of making a bipolar electrode
GB8420873D0 (en) * 1984-08-16 1984-09-19 Ici Plc Electrode for electrolytic cell
CN100487133C (zh) * 2002-07-20 2009-05-13 艾森生物(杭州)有限公司 基于阻抗的检测装置和方法
US7501301B2 (en) * 2004-03-10 2009-03-10 The Board Of Trustees Of The Leland Stanford Junior University Low cost fabrication of microelectrode arrays for cell-based biosensors and drug discovery methods
WO2018031888A1 (en) * 2016-08-12 2018-02-15 University Of Notre Dame Du Lac Closed bipolar electrode-enabled electrochromic detector for chemical sensing

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2013012498A1 (en) 2011-06-14 2013-01-24 Hemoshear, Llc Electric cell- substrate impedance sensing (ecis) of biological samples in shear stress flow
WO2016139566A1 (en) 2015-03-03 2016-09-09 Abdolahad Mohammad Electrical cell-substrate impedance sensor (ecis)

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Publication number Publication date
US10895546B2 (en) 2021-01-19
US20180164238A1 (en) 2018-06-14
DE102016224865B3 (de) 2018-05-03

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