DE102013201297A1 - A fluid storage unit and method of making a fluid storage unit - Google Patents

Abstract

The invention relates to a unit (104) for storing a fluid (102), in particular a reagent or an auxiliary for a biochemical analysis method, the unit (104) comprising a base body (108), a piston (110) and a closure (112) having. The base body (108) has a through channel (120) in which the fluid (102) is arranged. The base body (108) is impermeable to the fluid (102) and / or components of the fluid (102). The base body is designed to be connected in a fluid-tight manner to a receiving device (106) of a biochemical analysis unit. The through channel (120) extends from a first end to a second end. The piston (110) is mounted so that it can move axially in the through-channel (120). The piston (110) is designed to be fluid-tight against the base body (108). The piston (110) is impermeable to the fluid (102) and / or components of the fluid (102) and is accessible from the first end of the through-channel (120). The closure (112) is arranged at the second end of the through channel (120). The closure (112) is impermeable to the fluid (102) and components of the fluid (102). The closure (112) is connected to the base body (108) in a fluid-tight manner. The closure (112) is designed to burst when a pressure in the fluid (102) is greater than a burst pressure.

Description

State of the art

The present invention relates to a fluid storage unit, to a receptacle for receiving the fluid storage unit, to a system for providing the fluid, to a method of manufacturing the unit, and to a method of providing the fluid.

Modern analytical methods are increasingly using small units for laboratory analysis, which is constructed as a cartridge with components of a disposable laboratory. The reagents required for an analysis must be kept in this cartridge. The DE 10 2009 045 685 A1 in this context, describes a microfluidic chip having an expandable membrane which is expandable under volume displacement into a fluid reservoir to move fluid from the fluid reservoir through a fluid port access into a fluid channel of the microfluidic chip.

Disclosure of the invention

Against this background, the present invention provides a fluid storing unit, a receiving device for storing the fluid reserving unit, a fluid supplying system, a method of manufacturing the unit and a fluid supplying method according to the main claims , Advantageous embodiments emerge from the respective subclaims and the following description.

Depending on their nature, plastics can be permeable to certain substances while impermeable to other substances. When various substances are stored in a plastic analysis unit with several immediately adjacent chambers, volatile substances can diffuse through the plastic and volatilize or contaminate other substances stored in adjacent chambers.

In order to exclude the contamination by diffusing substances in reagents and excipients within an analysis unit of a biochemical analysis method, the reagents and excipients can be stored pre-portioned in diffusion-tight containers and these containers can be opened automatically only immediately before use and rearranged the reagents and excipients in a range of analysis become. In the analysis area, the reagents and excipients remain only for the duration of the analysis procedure. Then the whole analysis unit can be disposed of. In particular, volatile reagents and auxiliaries can be stored in diffusion-tight containers. The diffusion-tight containers may be diffusion-tight units with a diffusion-tight seal, which are opened in response to a rearrangement command so that the reagents and auxiliaries can flow into the analysis region. The units can be stored within a receiving device.

The invention relates to a unit for storing a fluid, in particular a reagent or an adjuvant for a biochemical analysis method, the unit having the following features:
a body having a passageway in which the fluid is disposed, the body being impermeable to the fluid and / or constituents of the fluid and adapted to be fluid tightly connected to a biochemical analysis unit receiving device, the passageway being from a first end a second end runs;
a piston axially movably supported in the passageway, the piston being fluid-tightly sealed with respect to the body and / or the piston being impermeable to the fluid and constituents of the fluid and being accessible from the first end of the passageway; and
a closure disposed at the second end of the passageway, the closure being impermeable to the fluid and / or constituents of the fluid and being fluid tightly connected to the body, the closure being configured to burst when pressure in the body Fluid is greater than a bursting pressure.

Furthermore, a receiving device for receiving a unit for storing a fluid according to the approach presented here is presented, wherein the receiving device has the following feature:
a receiving opening for receiving the base body, wherein the receiving opening is formed as a passageway from an outer side of the receiving device to an inside of the receiving device, wherein the receiving opening is adapted to fluid-tight seal on an outer surface of the base body, when the base body is arranged in the receiving opening ,

Furthermore, a system for providing a fluid for a biochemical analysis unit is presented which has the following features:
a receiving device according to the approach presented here, wherein the inside is fluidly connected to the analysis unit; and
a unit for storing a fluid according to the approach presented here, wherein the base body is arranged in the receiving opening and the outer surface of the base body is sealed fluid-tight against the receiving opening, wherein the main body is permanently connected to the receiving device or connectable and arranged the closure on the inside or can be arranged.

A method is presented for producing a unit for providing a fluid, the method having the following steps:
Providing a body having a passageway and a piston which is axially movably mounted in the passageway, wherein in the passageway the fluid can be arranged and the body is impermeable to the fluid and constituents of the fluid and is adapted to be fluid-tight with a receiving device of a biochemical Analysis unit to be connected, wherein the passageway extending from a first end to a second end, wherein the piston is carried out with respect to the base body fluid-tight sealed and the piston is impermeable to the fluid and / or constituents of the fluid and accessible from the first end of the passage channel is;
Filling at least a portion of the passageway with the fluid from the second end; and
Disposing a closure at the second end of the passageway, the closure being impermeable to the fluid and / or constituents of the fluid and fluid tightly connected to the body, the closure being configured to burst when pressure in the fluid is greater than a bursting pressure is to establish the unit for providing the fluid.

Furthermore, a method for providing a fluid for a biochemical analysis unit is presented, which comprises the following steps:
Providing a system for providing according to the approach presented herein; and
Moving the piston from the first end into the passageway until the pressure in the fluid is greater than the bursting pressure to provide the fluid at the second end of the passageway, on the inside.

A base body may be, for example, a hollow cylinder. A passageway may be a bore or generally an aperture in the body. The through-channel can lead in a straight line with essentially unchanged cross-section through the main body. The passageway may have an irregular cross section. Likewise, the base body may have a prismatic shape. In this case, the passageway may have as a cross section a reduced image of a cross section of the base body. The passageway can also be formed independently of the cross section of the body. For example, the body of the outside correspond to a polygon, while the passageway is cylindrical. As a result, for example, the unit can be marked over the cross section of the base body and insertion of the base body into the wrong receiving device can be prevented. Inside the unit, a piston standardized for use in differently shaped basic bodies can be arranged in order to be able to use identical parts in several different units. The piston may have a seal for sealing to the base body. The piston may be movable along the passageway. A cross-sectional area of the passageway may vary from unit to unit, depending on what is located in the passageway for a fluid. The cross-sectional area may be dependent on a required amount of the fluid in the biochemical analysis method. The amount of fluid may also be determined via a stroke length of the piston in the passageway. A closure may be a lid. The closure may also be a cap or a flap. The main body, the piston and the closure can be made of the same material sin. The main body, the piston and the closure can also be made of different materials. When bursting, for example, a joint between the closure and the main body may burst. Likewise, the closure can burst itself. The pressure may increase when the piston is pushed into the passageway towards the closure. A cradle may be an interface to receive a unit. The fluid can also be filled into a plurality of chambers within the through-channel. Then, several different species can be stored in a single port and co-pressed by the piston. When squeezing the species can be mixed.

The closure may be designed as a diffusion-tight film which is welded to the base body. Alternatively or additionally, the closure may have a predetermined breaking point. For example, the film may be a laminate of different types of plastic and / or metal foil. For closing, at least one layer of the film can be fused to the base body. A predetermined breaking point may be a predetermined weak point of the closure, which fails first, for example due to notch effect, when the pressure is greater than the bursting pressure.

The closure may be configured to close when the pressure in the fluid is less than the bursting pressure by a tolerance pressure. The closure can again be fluid-tight when the piston stops in one position, for example, because only a subset of the fluid is needed in the biochemical analysis. For example, the closure can muster restoring forces that close the closure again.

A length of the piston may be greater than or equal to a length of the passageway. Alternatively or additionally, the piston and the passageway may form a press fit. If the piston is longer than or equal in length to the body, the piston can be pushed over the entire length of the passageway without tools. A press fit may be an interference fit. If the piston has a slightly larger cross-section than the passageway, then the piston can seal to the body without requiring further sealing.

The piston may have, on a side facing the first end, an actuating surface which is aligned transversely to a direction of movement of the piston. Alternatively or additionally, the piston may have a depth stop, which is designed to limit a penetration depth of the piston in the through-channel. An actuating surface may, for example, be understood to mean a receptacle for a plunger for actuating the piston. Likewise, the actuating surface may provide a larger area for actuating the piston than the piston has cross-sectional area. This allows the piston to be depressed more easily. For example, the piston may have a thickening at an end protruding from the main body. A depth stop may be a functional surface on the piston that abuts another functional surface when the piston reaches its maximum planned penetration depth. The other functional surface may be arranged on the base body. The other functional surface can also be arranged on the receiving device.

The base body may have at least one locking device for non-detachable connection of the base body to the receiving device. Alternatively or additionally, the main body can have at least one stop surface in order to limit an insertion depth of the main body into the receiving device. Alternatively or additionally, the main body can have at least one handling surface for handling the unit by a handling system. A locking device may for example be a latching lug. The locking device can not be reached in the locked state manually or by means of an instrument, d. H. be hidden so that the body can not be removed from the cradle. The main body can also have an engagement surface for a locking device of the receiving device. Recording device and body can interlock. An abutment surface may abut against a further abutment surface of the receiving device when the main body is arranged at a designated place. A handling surface may for example be formed as a counterpart to a gripper. At the handling surface, the unit can be automatically grasped, for example, to be transported for filling or to be inserted into the receiving device.

The invention will now be described by way of example with reference to the accompanying drawings. Show it:

1 a block diagram of a system for providing a fluid for a biochemical analysis unit according to an embodiment of the present invention;

2 a flowchart of a method of manufacturing a unit for providing a fluid according to an embodiment of the present invention;

3 a flowchart of a method for providing a fluid for a biochemical analysis unit according to an embodiment of the present invention;

4 a sectional view of a section of a system for providing a fluid for a biochemical analysis unit according to an embodiment of the present invention; and

5 an illustration of a system for providing a fluid with two units according to an embodiment of the present invention.

In the following description of preferred embodiments of the present invention, the same or similar reference numerals are used for the elements shown in the various figures and similarly acting, wherein a repeated description of these elements is omitted.

1 shows a block diagram of a system 100 for providing a fluid 102 for a biochemical analysis unit according to an embodiment of the present invention. The fluid 102 is particularly useful as a reagent or excipient for a biochemical analysis method. For example, the fluid 102 Alcohol or an alcohol mixture containing at least 75% alcohol. The system 100 consists of one unit 104 for storing the fluid 102 and a recording device 106 , The unit has a main body 108 , a piston 110 and a closure 112 on. The cradle 106 has a receiving opening 114 , a provisioning chamber 116 and a channel 118 to a biochemical analysis unit, not shown, for carrying out a biochemical analysis method.

The unit 104 for storing the fluid 102 is in the cradle 106 undetachably inserted after the unit 104 has been produced. The unit 104 and the cradle 106 are fluid-tightly interconnected. The main body 108 the unit has a central passageway 120 in which the fluid 102 is arranged. The main body 108 is made of a material that is impermeable to the fluid 102 and components of the fluid 102 is. The passageway 120 extends from a first end to a second end of the body 108 , The piston 110 is mounted axially movable in the passageway. The piston 110 is opposite the main body 108 sealed fluid-tight. The piston 110 is made of a material that is impermeable to the fluid and constituents of the fluid. The piston 110 is accessible from the first end of the passageway. The closure 112 is at the second end of the passageway 120 arranged. The closure 112 is of a material that is impermeable to the fluid and / or constituents of the fluid. The closure 120 is fluid-tight with the main body 108 connected. The closure 112 is designed to burst when a pressure in the fluid 102 greater than a bursting pressure.

The receiving opening 114 is designed to be the main body 108 take. The receiving opening 114 is as a passageway from an outside of the cradle 106 to an inside of the receiving device 106 educated. The receiving opening 114 is adapted to an outer surface of the main body 108 fluid-tight seal when the body 108 in the receiving opening 108 is arranged.

The unit 104 can be made of a material that is diffusion-tight for solvents, especially alcohol. The material may in particular be a polymer. This allows in the unit 104 solvent-containing, especially alcoholic fluids or liquids and / or pastes are stored permanently lossless until they are needed. Also denaturing fluids can be protected in the unit from solvent vapors, especially alcohol vapors. The cradle 106 In turn, it may be made of a low cost, well mouldable material since the fluid and other fluids remain in the receptacle only for the duration of the biochemical analysis process and there is not enough time for diffusion processes.

By using a syringe container 104 For the storage of alcohol as fluid, an advantageous plastic (COC, PP, PE) or steel can be used. The plastic of the syringe container 104 can be independent of the plastic of the cartridge 106 to be selected. Thus, a low cost plastic, with high alcohol permeation rates in the design of the cartridge 106 used (eg PC).

By the actuation of the syringe 104 becomes the liquid 102 always expressed at 100% and there is no loss of expensive reagents in the LOC fluid network 116 , which leads to a cost optimization of expensive PCR primers. In addition, the liquid can 102 metered, resulting in an improved process. Small amounts (a few μl) of liquids 102 can be stored stable for a long time and provided 100% to the assay. This results in a quality improvement in the course of the assay.

By the movement of the piston 110 can be low viscosity liquids 102 stored and safely expressed. Expressing about a volume displacement represents a flexible and robust system. The syringes 104 can be used as extra reservoirs during the process on the cartridge 100 be used, whereby a reduction in size is made possible. By a two-chamber system inside the syringe 104 can liquids 102 inside the syringe 104 be mixed, thereby further reducing the size of the cartridge 100 is possible.

2 shows a flowchart of a method 200 for producing a unit for providing a fluid according to an embodiment of the present invention. The procedure 200 has a step 202 of providing a step 204 of filling and one step 206 of arranging. In step 202 the provision of a base body with a through-passage and a piston which is mounted axially movable in the passage, provided. In the passageway, the fluid can be arranged. The body is impermeable to the fluid and / or constituents of the fluid. The base body is designed to be connected in a fluid-tight manner to a receiving device of a biochemical analysis unit. The passageway extends from a first end to a second end. The piston is carried out with respect to the main body sealed fluid-tight and impermeable to the fluid and constituents of the fluid. The piston is accessible from the first end of the passageway. In step 204 the filling becomes the passageway of the second end filled with the fluid. In step 206 arranging a closure at the second end of the passageway is arranged. The closure is impermeable to the fluid and constituents of the fluid and is fluid tightly connected to the body to produce the unit for providing the fluid. The closure is configured to burst when a pressure in the fluid is greater than a bursting pressure.

3 shows a flowchart of a method 300 for providing a fluid for a biochemical analysis unit according to an embodiment of the present invention. The procedure 300 has a step 302 providing and a step 304 of moving on. In step 302 In providing, a system for providing is provided according to the approach presented here. In step 304 moving the piston is moved from the first end into the passageway until the pressure in the fluid is greater than the bursting pressure to provide the fluid at the second end of the passageway, on the inside.

4 shows a sectional view of a section of a system 100 for providing a fluid for a biochemical analysis unit according to an embodiment of the present invention. 4 thus shows a cross section of a clipped-in syringe 104 in the cartouche 106 , The system 100 corresponds to the system in 1 , In addition, the piston points 110 a length greater than a length of the main body 108 is. This is the piston 110 in the illustrated, filled state of the unit 104 with a free end over the main body 108 above. The piston 110 has an actuating surface at the free end 400 on. The actuating surface 400 is transverse to a direction of movement of the piston 110 aligned. The actuating surface 400 is many times larger than a cross-sectional area of the piston 110 , Due to the size of the actuating surface 400 will be moving the piston 110 too simplistic. At one edge of the actuating surface 400 is a collar 402 arranged. The collar 402 is transverse to the actuating surface 400 aligned and points in the direction of the recording device 106 , The collar 402 is about the actuating surface 400 running around. A cutting surface of the piston 110 , the actuating surface 400 and the collar 402 has the form of a capital letter T with serifs on the bar. This represents the piston 110 the trunk of the letter, the actuating surface 400 the dash and the collar 402 the serifs. The collar 402 is designed as a depth stop for the piston 110 to act. When operating the piston 110 can the piston 110 so far into the body 108 be pressed until a stop surface 404 of the collar 402 on an outside of the receiving device 106 is applied.

The main body 108 is executed in this embodiment as a hollow cylinder. The main body has a locking device 406 for permanent connection of the body 108 with the cradle 106 on. The locking device 406 is in this embodiment as a plurality of springs 406 running out of the main body 108 protrude. The feathers 406 are trained to insert the unit 104 in the cradle 106 each to be bent over and at a projection 408 the cradle 106 lock. For this purpose, the recording device 106 in the area of the passageway 114 Bags 410 on top of the tabs 408 form. Between the bags 410 and the inside is a sealing surface 412 arranged at the unit 104 the passageway 114 seals fluid-tight when the unit 104 with the cradle 106 connected is. The feathers 406 lie in the inserted state on the inside of the side of the bags 410 and thus represent the stop surface for limiting the insertion depth of the body 108 , Between the outside and the pockets 410 has the passageway 114 Spacers on to room for inserting the springs 406 provide. When the springs 406 in the pockets 410 are arranged, they extend diagonally through the pockets 410 and prevent removal of the unit 104 from the recording device 106 , The passageway in the body 108 is like in 1 through a foil as a closure 112 covered. The film is firmly bonded to the base body 108 connected. To a controlled bursting of the closure 112 to reach, the film may have a predetermined breaking point. Likewise, for example, a portion of a joint between the closure 112 and the body 108 be performed weaker than the rest of the joint, so that the closure in the subregion fails controlled.

In contrast to 1 has the receiving device 106 no supply chamber here. The closure 112 is directly in the channel 118 arranged while the main body 104 in the channel 118 protrudes.

In other words, the reagents are in so-called syringes 104 bottled. 4 shows a cross section of a clipped-in syringe 104 in the cartouche 106 , These syringes 104 can outside the cartridge 106 be filled and then through an adhesive film 112 sealed. All syringes 104 be in the cartridge during the production process 106 clipped, with alternative mounting options as well are possible, such as screws or clamps. The syringes 104 can through a lid, as in 5 be protected against unintentional operation during transport. When supplying the fluid pushes the DxU (DxU = Diagnostic Unit) with a force F on the piston 110 and presses the syringe with it 104 , A DxU can be understood as an evaluation device into which the cartridge is inserted and through which the cartridge is operated. The evaluation unit also provides the interface to the operator in which the results are displayed. The amount of reagent that is provided may be via the travel path and through a defined stop 404 in the cartouche 106 be determined. In addition, by the diameter of the piston 110 the volume of the syringe 104 be varied. For the operation of the piston 110 are between DxU and cartouche 100 no sealing elements necessary. The sealing elements are already in the cartridge 106 and syringe 104 integrated.

5 shows a representation of a system 100 for providing a fluid with two units 104 according to an embodiment of the present invention. The units 104 correspond to the unit in 4 , Both units 104 are arranged side by side. The units 104 are in a common recording device 106 plugged in and permanently locked. The cradle 106 has two side-by-side through holes. The through holes may open into a common mixing chamber to the fluids of both units 104 to mix. Likewise, the through-holes can also lead to a respective provision chamber in order to be used separately for the biochemical analysis method. To the units 104 to protect against unintentional actuation, the actuating surfaces 400 through a protective hood 500 covered. The protective hood 500 is at the cradle 106 attached and is intended to be before using the system 100 be removed so that the actuating surfaces are exposed. The protective hood 500 protects the units 104 also against environmental influences, especially soiling. This allows an analyzer to deploy the analyzer with the system attached thereto 100 to be kept clean for deployment.

In other words shows 5 a LOC (Lab On Chip) cartridge 100 with integrated syringes 104 for reagent pre-storage. In lab-on-chip (LOC) products or so-called micro-fluidic platforms (μTAS), medical and biological fluids are processed on a carrier to analyze patient samples for the presence of pathogens and bacteria. The lab-on-chip platforms can be constructed as so-called cartridges, which receive and process the patient sample as a disposable article. For the process flow on the cartridge liquids are needed, which can either be stored on the cartridge or can be added later for the process flow by an operator. The subsequent use of reagents, including alcoholic substances can be avoided by the approach presented here, as this is provided shortly before the end of the assay. As a result, the high permeation rates of the alcohol in inexpensive plastics play no role.

The approach presented here allows a long-term stable storage of all reagents of a lab-on-chip assay on the cartridge 100 , Particularly alcoholic reagents or reagents with small amounts can be used with the units presented here 104 for storing safely within the LOC cartridge 100 be stored. The system shown 100 ensures storage during transport and allows easy opening and provision of reagents in the application. All pistons of the syringes 104 a cartouche 106 can be connected via a bar, which is pushed down by the DxU and thus the syringes 104 emptied simultaneously.

The embodiments described and shown in the figures are chosen only by way of example. Different embodiments may be combined together or in relation to individual features. Also, an embodiment can be supplemented by features of another embodiment.

Furthermore, method steps according to the invention can be repeated as well as carried out in a sequence other than that described.

If an exemplary embodiment comprises an "and / or" link between a first feature and a second feature, then this is to be read so that the embodiment according to one embodiment, both the first feature and the second feature and according to another embodiment either only first feature or only the second feature.

QUOTES INCLUDE IN THE DESCRIPTION

This list of the documents listed by the applicant has been generated automatically and is included solely for the better information of the reader. The list is not part of the German patent or utility model application. The DPMA assumes no liability for any errors or omissions.

Cited patent literature

• DE 102009045685 A1 [0002]

Claims (10)

Unit ( 104 ) for storing a fluid ( 102 ), in particular a reagent or an adjuvant for a biochemical analysis method, wherein the unit ( 104 ) has the following features: a base body ( 108 ) with a through-channel ( 120 ) in which the fluid ( 102 ), wherein the base body ( 108 ) impermeable to the fluid ( 102 ) and / or constituents of the fluid ( 102 ) and is adapted to be fluid-tight with a receiving device ( 106 ) to a biochemical analysis unit, the passageway ( 120 ) extends from a first end to a second end; a piston ( 110 ) located in the passageway ( 120 ) is mounted axially movable, wherein the piston ( 110 ) relative to the main body ( 108 ) is executed sealed fluid-tight and the piston ( 110 ) impermeable to the fluid ( 102 ) and / or constituents of the fluid ( 102 ) and from the first end of the passageway ( 120 ) is accessible; and a closure ( 112 ) located at the second end of the passageway ( 120 ), wherein the closure ( 112 ) impermeable to the fluid ( 102 ) and / or constituents of the fluid ( 102 ) and fluid-tight with the main body ( 108 ), the closure ( 112 ) is configured to burst when a pressure in the fluid ( 102 ) is greater than a bursting pressure. Unit ( 104 ) according to claim 1, wherein the closure ( 112 ) is designed as a diffusion-tight film, which with the main body ( 108 ) is welded and / or the closure ( 112 ) has a predetermined breaking point. Unit ( 104 ) according to one of the preceding claims, in which the closure ( 112 ) is adapted to close when the pressure in the fluid ( 102 ) is less than the bursting pressure by a tolerance pressure. Unit ( 104 ) according to one of the preceding claims, in which a length of the piston ( 110 ) greater than or equal to a length of the passageway ( 120 ) and / or in which the piston ( 110 ) and the passageway ( 120 ) form a press fit. Unit ( 104 ) according to one of the preceding claims, in which the piston ( 110 ) on an end facing the first end of an actuating surface ( 400 ), which transversely to a direction of movement of the piston ( 110 ) is aligned and / or the piston ( 110 ) a depth stop ( 404 ), which is adapted to a penetration depth of the piston ( 110 ) in the passageway ( 120 ) to limit. Unit ( 104 ) according to one of the preceding claims, in which the basic body ( 108 ) at least one locking device ( 406 ) for the permanent connection of the basic body ( 108 ) with the receiving device ( 106 ) and / or the main body ( 108 ) has at least one stop surface to an insertion depth of the body ( 108 ) in the receiving device ( 106 ) and / or the basic body ( 108 ) at least one handling surface for handling the unit ( 104 ) by a handling system. Receiving device ( 106 ) to record a unit ( 104 ) for storing a fluid ( 102 ) according to one of claims 1 to 6, wherein the receiving device ( 106 ) has the following feature: a receiving opening ( 114 ) for receiving the basic body ( 108 ), wherein the receiving opening ( 114 ) as a passageway from an outside of the receiving device ( 106 ) to an inside of the receiving device ( 106 ), wherein the receiving opening ( 114 ) is adapted to on an outer surface of the base body ( 108 ) fluid-tight seal when the main body ( 108 ) in the receiving opening ( 114 ) is arranged. System ( 100 ) for providing a fluid ( 102 ) for a biochemical analysis unit having the following features: a receiving device ( 106 ) according to claim 7, wherein the inside is fluidly connected to the analysis unit; and a unit ( 104 ) for storing a fluid ( 102 ) according to one of claims 1 to 6, wherein the main body ( 108 ) in the receiving opening ( 114 ) is arranged and the outer surface of the base body ( 108 ) against the receiving opening ( 114 ) is sealed fluid-tight, wherein the main body ( 108 ) insoluble with the receiving device ( 106 ) or connectable and the closure ( 112 ) is arranged on the inside or can be arranged. Procedure ( 200 ) for producing a unit ( 104 ) for providing a fluid ( 102 ) according to any one of claims 1 to 6, wherein the method ( 200 ) comprises the following steps: providing ( 202 ) of a basic body ( 108 ) with a through-channel ( 120 ) and a piston ( 110 ) located in the passageway ( 120 ) is mounted axially movable, wherein in the passageway ( 120 ) the fluid ( 102 ) can be arranged and the main body ( 108 ) impermeable to the fluid ( 102 ) and components of the fluid ( 102 ) and is adapted to be fluid-tight with a receiving device ( 106 ) to a biochemical analysis unit, the passageway ( 120 ) extends from a first end to a second end, wherein the piston ( 110 ) relative to the main body ( 108 ) is executed sealed fluid-tight and the Piston ( 110 ) impermeable to the fluid ( 102 ) and / or constituents of the fluid ( 102 ) and from the first end of the passageway ( 120 ) is accessible; To fill ( 204 ) at least a part of the through-channel ( 120 ) with the fluid ( 102 ) from the second end; and arranging ( 206 ) of a closure ( 112 ) at the second end of the passageway ( 120 ), the closure ( 112 ) impermeable to the fluid ( 102 ) and / or constituents of the fluid ( 102 ) and fluid-tight with the main body ( 108 ), the closure ( 112 ) is configured to burst when a pressure in the fluid ( 102 ) is greater than a burst pressure to the unit ( 104 ) for providing the fluid ( 102 ). Procedure ( 300 ) for providing a fluid ( 102 ) for a biochemical analysis unit comprising the following steps: 302 ) of a system ( 100 ) for providing according to claim 8; and moving ( 304 ) of the piston ( 110 ) from the first end into the through-channel ( 120 ) until the pressure in the fluid ( 102 ) is greater than the burst pressure to the fluid ( 102 ) at the second end of the passageway ( 120 ) to provide on the inside.

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