DE102010022903B4 - Compilation or kit comprising live microorganisms with activity indicator - Google Patents

Compilation or kit comprising live microorganisms with activity indicator Download PDF

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Publication number
DE102010022903B4
DE102010022903B4 DE102010022903A DE102010022903A DE102010022903B4 DE 102010022903 B4 DE102010022903 B4 DE 102010022903B4 DE 102010022903 A DE102010022903 A DE 102010022903A DE 102010022903 A DE102010022903 A DE 102010022903A DE 102010022903 B4 DE102010022903 B4 DE 102010022903B4
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kit according
microorganisms
metabolic activity
composition
kit
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DE102010022903A1 (en
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Herbert Rehms
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Dynamics Ges fur Umweltschutz und Biolog Verfahrenstechnik Mbh & Co KG
Dynamics Gesellschaft fur Umweltschutz und Biologische Verfahrenstechnik Mbh & Co KG
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Dynamics Ges fur Umweltschutz und Biolog Verfahrenstechnik Mbh & Co KG
Dynamics Gesellschaft fur Umweltschutz und Biologische Verfahrenstechnik Mbh & Co KG
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/20—Bacteria; Culture media therefor
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K63/00—Receptacles for live fish, e.g. aquaria; Terraria
    • A01K63/04—Arrangements for treating water specially adapted to receptacles for live fish
    • A01K63/045—Filters for aquaria

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  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Health & Medical Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Biotechnology (AREA)
  • Environmental Sciences (AREA)
  • Organic Chemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Zoology (AREA)
  • Genetics & Genomics (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Microbiology (AREA)
  • Biomedical Technology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Virology (AREA)
  • Marine Sciences & Fisheries (AREA)
  • Animal Husbandry (AREA)
  • Biodiversity & Conservation Biology (AREA)
  • Medicinal Chemistry (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)

Abstract

Zusammengefasst wird ein Kit vorgeschlagen, in dem trocken formulierte Mikroorganismen mit einem Indikator versehen werden, der es dem Verbraucher erlaubt, nach erfolgter Aktivierung die biologische Aktivität und Stoffwechselleistung der Mikroorganismen festzustellen. Der Verbraucher kann so vor dem Gebrauch sehr einfach, z. B. über einen Farbumschlag feststellen, ob die Kulturen noch ausreichend vital sind.In summary, a kit is proposed in which dry-formulated microorganisms are provided with an indicator which allows the consumer, after activation, to ascertain the biological activity and metabolic activity of the microorganisms. The consumer can so very easy before use, for. B. determine via a color change, if the cultures are still sufficiently vital.

Description

Die Neuerung bezieht sich auf ein Kit mit lebenden Mikroorganismen für den Einsatz in Teichen und Aquarien, deren Stoffwechselaktivität vor dem Einsatz beim Kunden sichtbar gemacht wird.The innovation relates to a kit with living microorganisms for use in ponds and aquariums whose metabolic activity is made visible before use at the customer.

Für die Pflege von Teichen gibt es ein breites Sortiment von Starterkulturen zur Verbesserung der biologischen Wasserreinigung. Dabei besteht das Problem, dass der Anwender nicht beurteilen kann, ob das gekaufte Produkt noch lebende und stoffwechselaktive Organismen in ausreichender Anzahl mit ausreichender Stoffwechselaktivität enthält.For the care of ponds, there is a wide range of starter cultures to improve the biological water purification. There is the problem that the user can not judge whether the purchased product still contains living and metabolically active organisms in sufficient numbers with sufficient metabolic activity.

Durch die erfindungsgemäße Neuerung können lange haltbare, trocken formulierte, bei Lagerung und Transport inaktive Starterkulturen hergestellt werden, die erst beim Kunden aktiviert werden und deren Aktivität vor der Anwendung im Teich oder Aquarium nachgewiesen wird. Der Kunde braucht dann nicht mehr zu befürchten, Starterkulturen anzuwenden, die nicht ausreichend aktiv sind. Er kann mit seiner Anwendung beginnen, wenn er sich selber visuell von der Stoffwechselaktivität überzeugt hat.By the innovation according to the invention long-lasting, dry-formulated, in storage and transport inactive starter cultures can be prepared, which are activated only at the customer and their activity is detected before use in the pond or aquarium. The customer then no longer has to worry about using starter cultures that are not sufficiently active. He can start his application if he has visually convinced himself of the metabolic activity.

Claims (8)

Zusammenstellung oder Kit, umfassend eine Starterkultur mit lebenden Mikroorganismen für Teiche, Aquarien und deren Filter, sowie zusätzlich umfassend Mittel oder Zusammensetzung(en) zum Nachweisen oder Sichtbarmachen der Stoffwechselaktivität der Mikroorganismen der Starterkultur vor deren bestimmungsgemäßem Gebrauch.An assembly or kit comprising a live microorganism starter culture for ponds, aquariums and their filters, and additionally comprising means or composition (s) for detecting or visualizing the metabolic activity of the microorganisms of the starter culture prior to their intended use. Zusammenstellung oder Kit nach Anspruch 1, dadurch gekennzeichnet, dass zum Nachweis der Stoffwechselaktivität farblose Enzymsubstrate verwendet werden, die einen Farbstoff enthalten, der enzymatisch freigesetzt werden kann und dann eine sichtbare Färbung der Lösung hervorruft.Composition or kit according to Claim 1, characterized in that colorless enzyme substrates which contain a dye which can be released enzymatically and then cause a visible coloration of the solution are used to detect the metabolic activity. Zusammenstellung oder Kit nach Anspruch 1, dadurch gekennzeichnet, dass zum Nachweis der Stoffwechselaktivität ein pH-Indikator enthalten ist, der die Bildung von Säure, die über den Stoffwechselprozess frei wird, über einen Farbumschlag nachweist.Composition or kit according to claim 1, characterized in that for the detection of metabolic activity, a pH indicator is included, which detects the formation of acid, which is released through the metabolic process, via a color change. Zusammenstellung oder Kit nach Anspruch 1, dadurch gekennzeichnet, dass der Nachweis der Stoffwechselaktivität über feine Partikel erfolgt, die durch Gasproduktion durch die Mikroorganismen in sichtbare Bewegung versetzt werden.Assortment or kit according to claim 1, characterized in that the detection of metabolic activity via fine particles, which are put into visible motion by gas production by the microorganisms. Zusammenstellung oder Kit nach einem der vorherigen Ansprüche, dadurch gekennzeichnet, dass der Nachweis der Stoffwechselaktivität mithilfe eines Gärröhrchens erfolgt, in dem durch die Stoffwechselaktivität entstandene Gase aufgefangen und nachgewiesen werden.Composition or kit according to one of the preceding claims, characterized in that the detection of metabolic activity by means of a fermentation tube takes place in the resulting by the metabolic activity gases are detected and detected. Zusammenstellung oder Kit nach einem der vorherigen Ansprüche, dadurch gekennzeichnet, dass eine Trockenformulierung der Mikroorganismen und eine wässrige Aktivierungslösung getrennt in einer Multifunktionsflasche verpackt sind.Assortment or kit according to one of the preceding claims, characterized in that a dry formulation of the microorganisms and an aqueous activating solution are packed separately in a multi-functional bottle. Zusammenstellung oder Kit nach einem der vorherigen Ansprüche, dadurch gekennzeichnet, dass eine Trockenformulierung der Mikroorganismen durch Zugabe von Teich- oder Aquarienwasser aktiviert wird.Composition or kit according to one of the preceding claims, characterized in that a dry formulation of the microorganisms is activated by the addition of pond or aquarium water. Zusammenstellung oder Kit nach einem der vorherigen Ansprüche, dadurch gekennzeichnet, dass eine Trockenformulierung der Mikroorganismen durch Zugabe von oder Zusammengeben mit Leitungswasser, Wasser oder einer wässrigen Nährlösung aktiviert wird.Assortment or kit according to one of the preceding claims, characterized in that a dry formulation of the microorganisms is activated by adding or combining with tap water, water or an aqueous nutrient solution.
DE102010022903A 2009-11-04 2010-06-07 Compilation or kit comprising live microorganisms with activity indicator Active DE102010022903B4 (en)

Priority Applications (1)

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DE102010022903A DE102010022903B4 (en) 2009-11-04 2010-06-07 Compilation or kit comprising live microorganisms with activity indicator

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
DE202009015000.3 2009-11-04
DE202009015000 2009-11-04
DE102010022903A DE102010022903B4 (en) 2009-11-04 2010-06-07 Compilation or kit comprising live microorganisms with activity indicator

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DE102010022903A1 DE102010022903A1 (en) 2011-08-04
DE102010022903B4 true DE102010022903B4 (en) 2013-06-27

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Citations (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE4316394C1 (en) * 1993-05-17 1994-06-23 Biotest Ag A method of optically detecting microorganisms, their identification and antibiotic susceptibility testing using a redox indicator system comprising methylene blue and resazurin
WO1999032603A1 (en) * 1997-12-22 1999-07-01 Aquaria Inc. Bacterial nitrite oxidizer and method of use thereof
DE10047709A1 (en) * 2000-09-25 2002-05-02 Thomas Willuweit Process for the treatment of water using microorganisms
DE20207502U1 (en) * 2002-05-14 2003-06-18 Söll Holding GmbH, 61476 Kronberg Photoprotected starter culture for microbiological treatment of water, soil, sediments and/or sludges, comprises live chemolithoautotrophic bacteria immobilized in a matrix coated with pigments and/or adsorbents
DE60109441T2 (en) * 2000-05-19 2006-04-13 Aquaria, Inc., Moorpark AMMONIA-OXIDATING BACTERIA
WO2006044499A2 (en) * 2004-10-14 2006-04-27 Novozymes Biologicals, Inc Consortium of nitrifying bacteria

Patent Citations (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE4316394C1 (en) * 1993-05-17 1994-06-23 Biotest Ag A method of optically detecting microorganisms, their identification and antibiotic susceptibility testing using a redox indicator system comprising methylene blue and resazurin
WO1999032603A1 (en) * 1997-12-22 1999-07-01 Aquaria Inc. Bacterial nitrite oxidizer and method of use thereof
DE60109441T2 (en) * 2000-05-19 2006-04-13 Aquaria, Inc., Moorpark AMMONIA-OXIDATING BACTERIA
DE10047709A1 (en) * 2000-09-25 2002-05-02 Thomas Willuweit Process for the treatment of water using microorganisms
DE20207502U1 (en) * 2002-05-14 2003-06-18 Söll Holding GmbH, 61476 Kronberg Photoprotected starter culture for microbiological treatment of water, soil, sediments and/or sludges, comprises live chemolithoautotrophic bacteria immobilized in a matrix coated with pigments and/or adsorbents
WO2006044499A2 (en) * 2004-10-14 2006-04-27 Novozymes Biologicals, Inc Consortium of nitrifying bacteria

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