DE102007056759A1 - Blood sampling device i.e. syringe, for removing blood from patient e.g. human, has container device with two chambers for separating blood components, and separating unit with two separating parts for separating blood components - Google Patents

Abstract

The device (10a) has a container device (12a) comprising two chambers (14a, 16a) for separating blood components (18a, 20a) e.g. white blood corpuscle. A separating unit (22a) has two separating parts (24a, 26a) for separating the blood components, where the separating parts are movably and rotatable against each other. The separating unit is closable and/or openable and arranged between the chambers. An injecting piston (32a) forms the chamber (16a), where the injecting piston is relatively movable in an injecting cylinder (34a). A glass shear body (36a) is arranged in the chamber (14a).

Description

State of the art

It are already blood collection devices, such as syringes, with a container device, especially in medical applications.

The The invention is based on a blood sampling device according to the preamble of claim 1.

Advantages of the invention

The The invention is based on a blood sampling device with at least a container device.

It It is suggested that the container device has at least two chambers for the separation of blood components. In this case, a blood withdrawal preferably defines a recovery of a blood sample from a blood vessel, as example, a Artery, a vein and / or a jugular vein of a mammal. The extraction is preferably carried out by means of a cannula, wherein here under a "cannula" also a butterfly cannula, a Venous indwelling cannula and / or another, the expert appears appropriate as a blood collection agent to be understood. One for that suitable mammal represents in particular a human being, a dog, a cat, an odd-toed ungulate, like a horse, a pair of ungulates, like a camel, a cattle and / or a However, the blood sampling device can also be used for blood sampling be suitable for another animal. In this context is under a "container device" in particular a device with a container be understood, with a container an object having a cavity in its interior and / or in particular serves the purpose of expressing its content Environment or its environment and / or other components separate. Further, a container is in at least one operating state in the case of the blood sampling device for example, in a state of centrifugation against a Environment tight or impermeable for his Content. Under a "chamber" is here in particular a space or a cavity can be understood, which is a recording a substance, such as a solid, a gas and in particular a liquid, and particularly advantageous for receiving blood and / or blood components is provided. Blood defines here in particular whole blood, and blood components define here on the one hand a blood cake, therefore cellular components of the blood (red and white Blood cells), platelets and coagulation factors, and on the other a blood serum, and indeed in particular a clotted blood sample without blood cake. The serum contains about 91% water and 7% proteins (albumins and globulins), the the remaining 2 are electrolytes, nutrients and hormones. By the embodiment of the invention can separate storage of components, in particular blood components, which should not be mixed, can be achieved. Furthermore, results thereby a safe handling and a variable transportation possibility, in which a thorough mixing of the components can be effectively prevented, even if the blood sampling device, for example, at a Removal process of a sample from the blood collection device, on the head is turned.

Of Furthermore, it is proposed that the blood sampling device a Separation unit, which is used to separate the blood components is provided. In this context, under a "separating unit" in particular a Unit be understood, arranged at least one component is that for separating a component from a mixture of components is provided. The separating unit preferably has at least one Component unit, which consists of at least two components, reflected in at least one parameter, such as size, material, strength, permeability and / or separation performance. At least one of the components serves the separation. Particularly advantageous is the arrangement of two Component units. Furthermore, under "provided" specially equipped and / or designed to be understood. By arranging the separating unit can a separation of components constructively simple and efficient be designed.

Further it is advantageous if the separating unit has at least two separating agents having. In this context, under a "separating agent" in particular a Means, such as a partition, a sieve, a fiber composite (fabric, Wool), a porous Structure (rock, lime), a membrane and / or a filter, understood be that at least one component of a mixture of components according to at least one parameter, such as size, charge, shape, viscosity, temperature and / or a parameter which will be further useful to those skilled in the art, disconnects and / or this component in at least one operating condition separated from other components of the mixture holds. through of the separating agent can be the separation of components especially easy and reliable be achieved. Furthermore, the separating unit thus has an advantage variable modular design and is particularly flexible.

In a further embodiment of the invention, it is proposed that the separating GE are executed movable against each other. In this case, "movable" defines a movement of the separating means relative to one another in an axial direction and / or in a horizontal direction and / or in a radial direction.

Further It is proposed that the separating means rotatable against each other are arranged. Here, under the phrase "rotatably arranged" in this context in particular, be understood that at least one of the separating agents can be rotated in the circumferential direction and in particular relative to another separating agent. Particularly advantageous is one of Separator firmly connected to a control or piston rod and the further separating means relative to the piston rod and / or the other separating means arranged movably. At least two Separating agents may be preferred be brought in an operating condition in mutual agreement. This defines "in Cover one " Condition in which at least more than 50% of an area of both separating a common coverage area to have. By means of the rotatable arrangement of the separating means, the Separator unit easy to use and / or adjustable and / or be made adjustable.

advantageously, the separating unit is closable and / or openable. Under "closable" should in this context be understood in particular that the separating unit in at least an operating state is closed or that a content that is in a chamber adjacent to the separating unit, kept in the chamber in this operating state and an exit the contents of the chamber is prevented. Furthermore, an entrance one outside the substance in the chamber are largely suppressed. Of Further defined in this context "openable" that the separating unit in at least an operating state is open or that a content of a to the separating unit adjacent chamber unhindered in and out of the Chamber can move and / or this can be moved freely. An open operating state of the separating unit occurs in particular if at least two separating agents have a coverage area of more than 50% of their respective area to have. By realizing these two states can be advantageous different operating modes of the separating unit are set, which can be used differently, making the separating agent can perform both a separation and a separating function.

Of Furthermore, it may be advantageous if the separating unit at least includes a filter. In this context, under a "filter" in particular a The subject matter of the components, in particular solid, liquid and gaseous Components, due to their material properties, such as size, charge, Shape, viscosity and / or Temperature, separates from each other. For example, the filter can be off Polyethylene sulfone (PES), polyvinylidene fluoride (PVDF), polytetrafluoroethylene (PTFE) and / or preferably from a cellulose ester such as cellulose acetate be made. But he can also from another, the expert exist as meaningful filter material. The filter is preferred Pores on that permeable are for Liquids, Small molecules, such as ions and / or RNA, and / or macromolecules, such as proteins and / or DNA, and impermeable are for Cells, such as blood cells and / or bacteria, viruses, cell organelles and / or Zell scrap. Furthermore, the pores advantageously have a diameter on, for example, less than 1 micron, and preferably less than 0.2 μm. A separation principle can also be applied to a semipermeability of a Filters are based. The filter is only in one direction for one Fabric permeable. By this embodiment of the invention can be a simple and inexpensive Principle for an efficient separation can be used.

One simple structure can be advantageously achieved when the separating unit is arranged between the chambers. Under the phrase "between the chambers "should here in particular a spatial Arrangement to be understood. Furthermore, the separating unit as Separating element to be understood that in at least one operating condition alternatively and / or additionally as a connecting element for the two chambers are used.

moreover It is suggested that the blood collection device be a syringe plunger having at least one chamber. Here is under a "syringe plunger" in particular a component the blood sampling device to be understood during a Blood collection process in an axial direction up and down preferred can be moved in a cylinder. The syringe piston serves one Narrowing of a space in which a liquid, such as the blood or the blood components are located. Through the realization of the chamber In the syringe plunger, a compact construction arrangement can be achieved become. Furthermore, the syringe plunger can perform a dual function. To the one serves to build up pressure in the chamber in which it is moved, and on the other hand, its volume can be particularly advantageous as storage or Recording room can be used.

A preferred development consists in that the blood sampling device has a syringe barrel to which the syringe plunger is relatively movable. In this context, under a syringe barrel in particular a tube be understood chamber in which the syringe plunger can be moved in the preferred axial direction and / or enclosing the syringe plunger in a circumferential direction and / or leads or in which the syringe plunger is guided. Further, the syringe barrel serves as a receiving space of the blood in the process of taking blood. By means of the syringe barrel, a structurally simple guidance of the syringe plunger can be provided.

It it is also proposed that at least one of the chambers in at least an operating condition deviating from an atmospheric pressure having. In this case, the operating state preferably constitutes an operating state in which the chamber is completely unfilled from a liquid. In the case of the blood sampling device, this is the operating state, before the separating agent or the filter is opened and the liquid blood components in pour in the chamber of the syringe plunger. There is also the rule of an atmospheric pressure deviating pressure is preferred in the chamber, in the syringe plunger is trained. Defined atmospheric pressure Here, a mean atmospheric pressure of the atmosphere, for example elevation 101325 Pa. A different pressure here represents a negative pressure or a vacuum By means of deviating from an atmospheric pressure can constructively simply a good intake of blood components be reached in the chamber of the syringe plunger. Generally it would be also conceivable, the blood sampling device at a normal pressure to operate.

Of Furthermore, it is proposed that the blood sampling device at least a shear body has, which is arranged in at least one of the chambers. In In this context, a "shear body" should be understood to mean, in particular, a body which to apply shear or shear forces a sample object, such as blood cells, is provided. Here, shear defines a deformation of the sample object below Action of a force tangential to a side surface of the Sample object works. Due to the arrangement and use of shear bodies can a simple, effective and cost-effective stimulation of a production be achieved by metabolites. These metabolic products or the blood sampling device should preferably be in the field of treatment of inflammation be applied. Therefor should the recovered, enriched with the metabolite blood serum especially in the inflamed Area, like all on the body located joints, areas near the spine, perineural areas, muscles, Tendons, ligaments, Fascia and / or others that appear appropriate to one skilled in the art Areas are introduced or injected. An application is unsuitable for an application directly into the bloodstream, ie in veins and arteries, and in a lymphatic system.

Prefers the metabolites formed are involved in inflammatory reactions. As a result, the blood collection device for the production of anti-inflammatory Active ingredients are used. A preferred anti-inflammatory agent is an interleukin-1 receptor antagonist (IL-1Ra). The interleukin-1 receptor antagonist is a receptor antagonist that is specifically an effect of a protein Interleukin-1 (IL1) neutralized. Interleukin-1 belongs as one proinflammatory Schlüsselzytokin to the main inflammatory Signaling substances of the immune system. It becomes an inflammatory stimulus mainly formed by monocytes and macrophages. inflammatory stimuli can Bacteria, yeasts, autoantigen, but also any kind of foreign particles be. Also, the application of the shear forces through the shear body the blood cells stimulates an inflammatory reaction. The pro-inflammatory Effects of interleukin-1 are induced by binding to one for interleukin-1 mediates specific interleukin-1 receptor. The interleukin-1 receptor antagonist is also released by immune cells and comes in the body as a natural one Inhibitor for Interleukin-1. He plays a vital role in one Regulation of an interleukin-1-mediated inflammatory process. An effect of the interleukin-1 receptor antagonist is based on its having a Effect of interleukin-1 slows down and stops by replacing of interleukin-1 to the interleukin-1 receptor, that of a target cell is expressed, docks to this target cell and thus attaching Interleukin-1 prevents interleukin-1 from becoming its pro-inflammatory Effects can no longer convey. The interleukin-1 receptor antagonist is thus the anti-inflammatory Antagonist of Interleukin-1.

Advantageously, at least one shear body is formed by a ball. Particularly advantageous, the blood sampling device 240 Shear body in the form of balls on. A number of balls may also have another quantity that appears appropriate to one skilled in the art. The shear body in this case has a diameter between 2 and 4 mm, whereby here another, a specialist would appear to be reasonable diameter feasible. By a spherical shape, a particularly efficient and also gentle-acting shear body can be provided. The shear body or the ball may be made of a metal, a plastic, and especially before geous glass. By realizing the glass body, an easily manufactured, lightweight and cost-effective shear body can be achieved, which directly enables the provision of a cost-effective and easy-to-use blood sampling device.

In Another embodiment of the invention proposes that the shear body a smooth surface having. Under a "smooth Surface "should here in particular a surface understood be the surveys and / or wells with one of a Main extension of the surface having different extension, the elevations and depressions smaller than 0.1 mm. The inventive design can be a gentle and efficient treatment of the blood or blood components, as the blood cells, are provided by the cells can be exposed to moderate stress, but none destruction the cellular Structures, such as the cell membrane, leads.

Further It is proposed that at least one of the chambers has a capacity between 10 and 100 ml. Preferably, at least one chamber has a capacity of between 30 and 80 ml and particularly advantageous between 50 and 60 ml. This is under a "volume of volume" in particular the Volume is understood to be the maximum of a body, like a cavity and / or a chamber can be recorded. in this connection can be a filler solid, liquid and / or gaseous be. If a blood volume of 50 ml is taken from the patient, the result is yourself by treating the blood with the shear bodies Concentration of interleukin-1 receptor antagonist, 140 times higher is the concentration in an untreated sample. Thereby can processually simply an efficient enrichment of an active ingredient be achieved in a meaningful volume. In addition, a sufficient Volume present to help disperse a healthy portion of to carry out smaller sample volumes. These portioned samples can be kept and can for a later Find time to use. Further, the patient only needs blood once which is particularly user-friendly, especially in the case of an animal as a patient and protective for one Blood loss is. Furthermore, it is by use of the patient's own blood to get a fast method to the drug to win.

The Blood collection device is provided according to the guidelines of the law over Medical devices (Medical Devices Act - MPG) approved and used to become.

drawing

Further Advantages are shown in the following description of the drawing. In the drawing are exemplary embodiments represented the invention. The drawing, the description and the claims contain numerous features in combination. The specialist will the features also expediently individually consider and summarize to meaningful further combinations.

It demonstrate:

1 a blood sampling device for a blood sample,

2 the blood sampling device 1 after a centrifugation step,

3 the blood sampling device 1 during a separation process,

4 the blood sampling device 1 after the separation process and during the sampling,

5 the separating unit off 4 in a sectional view along the line VV,

6 an alternative separating unit with two separating agents,

7 the blood sampling device 1 with an alternative reinforced syringe plunger,

8th the blood sampling device 1 with alternative and separable syringe plunger,

9 another alternative blood sampling device for a blood sample,

10 the blood sampling device 8th after a centrifugation step,

11 the blood sampling device 8th during a separation process,

12 the blood sampling device 8th after the separation process and during the sampling and

13 another alternative blood sampling device for a blood sample.

Description of the embodiments

1 shows a blood sampling device 10a in the form of a syringe with a container device 12a that have two chambers 14a . 16a for the separation of blood components 18a . 20a having. The blood sampling device 10a has a syringe barrel 34a and a syringe plunger 32a on. At a lower end 42a the syringe barrel 34a to which the syringe plunger 32a is relatively movable, is a syringe cone 44a with an external thread 46a arranged over which a cannula 48a who have an au ßendurchmesser between 1.5 and 0.5 mm (17 to 25 gauge), with its internal thread 50a with the syringe barrel 34a is connectable. In general, however, a cannula with a different outer diameter would be conceivable. A cavity of the syringe barrel 34a forms the chamber 14a , which has a capacity of 60 ml. However, it would be conceivable in principle, another capacity between 30 and 80 ml. To monitor a filling process of the blood collection device 10a or the syringe barrel 34a can be on an outside of the syringe barrel 34a a scaling, not shown here, for example, in 10 ml increments, be provided. Furthermore, the syringe barrel 34a at an upper end 52a an opening 54a on, in which the syringe plunger 32a is introduced.

In addition, the blood sampling device 240 shear body 36a on that in the chamber 14a the syringe barrel 34a are arranged. From the shear bodies 36a are for the sake of clarity only individual in the 1 shown. The shear bodies 36a are from bullets 38a formed, which are made of glass and a smooth surface 40a exhibit. The shear bodies 36a Take about a volume of 10 ml of the 60 ml volume of the syringe barrel 34a one.

The syringe plunger 32a gets from the syringe barrel 34a in an axial direction 56a guided and forms the chamber 16a , The chamber 16a has a liquid fill volume smaller than the liquid fill volume of the chamber 14a the syringe barrel 34a , and prefers a volume between 50 and 55 ml. To the tip piston 32a is at a lower end 58a a sealing element 60a arranged in the form of a rubber sealing ring. The sealing element 60a is stuck with the syringe plunger 32a connected and can with the syringe plunger 32a in the axial direction 56a to be moved. Furthermore, the sealing element generates 60a through his interaction with an outer wall 62a of the syringe plunger 32a or a surface 64a the outer wall 62a and a surface 66a an interior wall 68a the syringe barrel 34a a static friction to a targeted movement of the syringe plunger 32a relative to the syringe barrel 34a , In addition, the sealing element seals 60a the chamber 14a the syringe barrel 34a against an outdoor space 70a or in relation to oneself in the outer space 70a located air.

Furthermore, it is at the bottom 58a of the syringe plunger 32a a separating unit 22a arranged to separate the blood components 18a . 20a is provided. The separating unit 22a is between the chambers 14a and 16a arranged and thus separates the chamber 14a the syringe barrel 34a from the chamber 16a of the syringe plunger 32a , Furthermore, the separating unit 22a separating means 24a . 26a on that as a filter 28a . 30a trained and each in a component unit 72a . 74a are arranged. Thus, the separating unit comprises 22a the filters 28a . 30a , This forms a separating agent 24a . 26a each with a barrier agent 76a . 78a one of the component units 72a . 74a , The barrier agent 76a . 78a are made of a lightweight plastic and largely impermeable to solids, liquids and / or gases.

Like in the 5 exemplary for the component unit 72a is shown, the component units 72a . 74a each a connecting element 80a . 82a in the form of a mounting ring made of a lightweight plastic over which the semicircular separating means 24a or the filter 28a and the semicircular barrier 76a are connected. Furthermore, the connecting element serves 80a the component unit 72a to, the component unit 72a with a surface 84a an interior wall 86a of the syringe plunger 32a to connect captively. Furthermore, the component unit 72a a spacer element 88a in the form of a spacer made of a lightweight plastic material containing the separating agent 24a or the barrier element 76a spaced apart from a piston rod 90a arranges. The spacer element 88a or the component unit 74a can be in the circumferential direction 112a opposite the piston rod 90a to be moved. The component unit 74a is fixed to the piston rod via a mechanism not specified here 90a connected. By the arrangement of the spacer element 88a or a mobility of the component unit 72a and / or the fixed connection of the component unit 74a with the piston rod 90a can the separating agents 24a . 26a be executed against each other movable and are thus arranged rotatably against each other. Furthermore, the separating agents 24a . 26a be rotatable relative to each other by closing and / or openable executed to each other. The separating agents 24a . 26a and the barrier means 76a . 78a are each formed by a semi-circular cutout. With oppositely arranged separating agents 24 . 26a or barrier agents 76a . 78a , ie separating agent 24a via barrier agents 78a and barrier agents 76a via separating agent 26a , the separating unit can be converted by a rotation of 180 ° from a closed to an open position.

In 6 is an alternative separating unit 22a ' or an alternative component unit 72a ' shown. The component unit, not shown 74a ' is analogous to the component unit 72a ' executed. Here are the separating agents 24a ' . 24a '' or the filters 28a ' . 28a '' and the barrier means 76a ' . 76a '' formed by four pitch circle segments, each of which has an angle α of 90 °. With the same arrangement arranged as the separating agent 24 ' with the barrier agent 78 ' , the separating agent 24a '' with the barrier agent 78 '' , the separating agent 26a ' with the barrier agent 76a ' and the separating agent 26a '' with the barrier agent 76a '' , the separator unit can 22a ' be transferred by a rotation of 90 ° from a closed to an open position.

In addition, the syringe plunger 32a at an upper end 92a an opening 94a on, through which the piston rod 90a is guided. The piston rod 90a extends in the axial direction 56a from the separating unit 22a or the lower end 58a of the syringe plunger 32a through the chamber 16a over the opening 94a out and ends in a handle element 96a , Further, at the upper end 92a of the syringe plunger 32a a coupling device 98a in the form of a cone with external thread 100a arranged. On the external thread 100a the coupling device 98a is a closure element 102 with an internal thread 104a screwed.

In addition, the chamber shows 16a in an operating state, before the opening of the separating unit, a deviating from an atmospheric pressure, namely a negative pressure.

The following is an application of the blood sampling device 10a described. At the beginning of the blood collection process is the syringe plunger 32a at one of the lower ends 52a the syringe barrel 34a near position, which corresponds to a position known to those skilled in the beginning of a blood sample. This is in the chamber 14a the syringe barrel 34a only the shear bodies 36a and air (not shown). By means of the cannula 48a a vein of a mammal or a patient (not shown) is punctured and it is delivered to the patient by retracting the syringe plunger 32a relative to the syringe barrel 34a in a manner known to those skilled taken a volume of 50 ml of blood (see 1 ). After the blood collection, the cannula becomes 48a removed and the syringe cone 44a is by turning a female thread 106a a protection device 108a in the form of a protective cap on the external thread 46a the syringe cone 44a locked. The blood is in the chamber 14a the syringe barrel 34a and is determined by the arrangement of elements ( 24a . 26a . 28a . 30a . 76a . 78a ) of the separating unit 22a limited to these. This is the separating agent 24a the component unit 72a corresponding to the barrier agent 78a the component unit 74a arranged and the separating agent 26a the component unit 74a corresponding to the barrier agent 76a the component unit 72a , The barrier agent 76a . 78a prevent blood from passing through the separating means 24a . 26a or the filters 28a . 30a ,

The blood cells in the blood were at the blood collection by the suction pressure with increased pressure on the shear bodies 36a over crowded. This interaction of the blood cells with the balls 38a or their smooth surface 40a means for the blood cells a stress situation that comes close to an inflammatory process. In response to this stimulation, the blood cells form metabolic products (interleukin-1 and interleukin-1 receptor antagonist), which appear in inflammatory reactions. In order to achieve a sufficient enrichment of the blood with these metabolic products or, preferably, the accumulation of the anti-inflammatory interleukin-1 receptor antagonist, the blood taken off is in the blood sampling device 10a incubated for 2 to 8 hours at 37 ° C in an incubator.

After the incubation period, the blood collection device 10a removed from the incubator and centrifuged with a centrifuge at room temperature for a time sufficient to cause the shear bodies 36a or the solid blood components 18a , the blood cake, to sediment (for example, 10 minutes at 5000 rpm). After centrifugation, three layers have formed. At a splash bottom 110a first have the heavy shear body 36a discontinued, followed by the cellular blood components 18a or the blood cake and finally the liquid blood components 20a , the blood serum, accumulated (see 2 ).

Now to the blood serum or the liquid blood components 20a from the solid, cellular blood components 18a and the shear bodies 36a to disconnect, the piston rod becomes 90a over the handle element 96a 180 ° in the circumferential direction 112a turned. This also makes the component unit 74a firmly with the piston rod 90a connected, rotated and the separating means 24a . 26a or the filters 28a . 30a the component units 72a . 74a come together to cover each other. Now the syringe plunger 32a pushed back to the starting position of the blood collection and thereby reduces the volume of 60 ml of the chamber 14a the syringe barrel 34a , the liquid blood components can 20a the filters 28a . 30a pass and the blood serum passes, simplified by the negative pressure in the chamber 16a prevails, in the chamber 16a of the syringe plunger 32a , The cellular or solid blood components 18a and the shear bodies 36a be from the filters 28a . 30a retained and remain in the chamber 14a (please refer 3 ).

Are the liquid blood components 20a or the blood serum completely separated, the component unit 74a by turning the piston rod 90a closed again. As in 4 The serum can now be shown through an opening 114a the coupling device 98a be removed. For this purpose, the closure element 102 from the cone of the coupling device 98a unscrewed, and there will be a sample syringe 116a or reinjection syringe with a inner thread 118a on the external thread 100a the cone of the coupling device 98a screwed. By retracting a piston 120a in the axial direction 56a the sample syringe 116a now become the liquid blood components 20a out of the chamber 16a of the syringe plunger 32 taken. The acceptance step is repeated until the chamber 16a emptied and all serum was taken. To facilitate the serum decrease, the chamber 16a of the syringe plunger 32a when changing from a full sample syringe 116a to a still unfilled sample syringe 116a to equalize the pressure across the opening 114a be filled with air. The thus obtained 6 to 8 sample syringes with the enriched with Interleukin-1 receptor antagonist blood serum of a patient can now be used for repatriation in the patient, they can be stored for up to 7 months at -18 to -20 ° C.

In the 7 to 13 are alternative embodiments of the blood sampling device 10a shown. Essentially, the same components, features and functions are always numbered the same. To distinguish the embodiments, however, the reference numerals of the embodiments, the letters a and e are added. The following description is essentially limited to the differences from the embodiment in the 1 to 5 , wherein with respect to the same components, features and functions on the description of the embodiment in the 1 to 5 can be referenced.

7 shows a blood sampling device 10b in the form of a syringe with a container device 12b that have two chambers 14b . 16b for the separation of blood components 18b . 20b having. Here are for a centrifugation to a stabilization of a syringe plunger 32b the Blutentnahmevor direction 10b stabilizing elements 122b periodically 124b in the axial direction 56b distributed in the syringe plunger 32b brought in. These stabilizing elements 122b have recesses distributed on their surface 126b on that a passage of the liquid blood components 20b allow. Furthermore, a clamping element 128b , For example, made of rubber, be provided before the centrifugation around the circumference of the syringe plunger 32b is stretched and keeps it in a fixed axial position during centrifugation. Furthermore, the clamping element is used 128b as a stop at the top 52b the syringe barrel 34b ,

In 8th is a blood collection device 10c in the form of a syringe with a container device 12c that have two chambers 14c . 16c for the separation of blood components 18c . 20c has shown. This can be a syringe barrel 34c and a syringe plunger 32c the blood collection device 10c after a blood sample and prior to centrifugation are separated. For this purpose has the blood collection device 10c or the syringe plunger 32c a protection device 130c , The protection device 130c has a protective agent 132c , formed as a protective cover, on, after removing the syringe plunger 32c at the upper end 52c on the opening 54c the syringe barrel 34c is plugged. The syringe barrel 34c can now be centrifuged. Furthermore, the protection device 130c a protective film 134c on, after the separation of the syringe plunger 32c from the syringe barrel 34c on one side 136c remains in the assembled state of the blood sampling device 10c in the chamber 14c protrudes. This protective film 134c serves to the separating unit 22c or the filters 28c . 30c the separating unit 22c also during separation from the syringe barrel 34c to keep sterile. After centrifugation, the protective agent 132c out of the opening 54c the syringe barrel 34c removed, the protective film 134c is from the syringe plunger 32c sterile dissolved and the syringe plunger 32c gets into the opening 54c the syringe barrel 34c used sterile. The liquid blood components 20c are then passed through the filters 28c . 30c not only from the solid, cellular blood components 18c and the shear bodies 36c separately, but they are additionally filtered sterile.

It would also be possible, however, between the chambers 14c and 16c arranged separating unit 22c in component units 72c and 74c to separate. However, this would be on the one hand to sterile conditions in a separation, storage and centrifugation of the blood sampling device 10c to pay attention. On the other hand, the component unit would have to 74c that the syringe barrel 34c is associated with the protective means 132c against leakage of serum from the chamber 14c be covered, and ultimately would need the blood collection device 10c without a negative pressure in the syringe plunger 32c work. Alternatively, the entire separating unit could also be used 22c on the syringe barrel 34c remain, but this would have to ensure that the component units 72c and 74c during the Zentrifugationsvorgangs remain in their closed position to each other. For a not described in detail locking unit on the separating unit 22c be provided.

The 9 to 12 show an alternative blood sampling device 10d or show the course of a blood workup of this blood collection device 10d on. The blood sampling device 10d in the form of a syringe with a container device 12d has two chambers 14d . 16d for the separation of blood components 18d . 20d and a syringe plunger 32d who has the two chambers 14d and 16d includes, on. At a lower end 42d of the syringe plunger 32d is a syringe cone 44d is arranges over which a cannula 48d with the syringe plunger 32d is connectable. A cavity of the syringe plunger 32d has a capacity of 60 ml. At an upper end 176d is an opening 138d arranged in which a piston rod 90d is introduced. The upper end 140d the piston rod 90d is with a syringe barrel 142d captive connected. The syringe barrel 142d leads the syringe plunger 32d in an axial direction 56d and points at a lower end 144d a sealing element 60d in the form of a sealing lip. The sealing element 60d is stuck to the syringe barrel 142d connected and is with this in the axial direction 56d movable. Furthermore, the sealing element generates 60d for a targeted movement of the syringe plunger 32d relative to the syringe barrel 142d a stiction. In addition, the sealing element seals 60d a cavity 146d between the syringe plunger 32d and the syringe barrel 142d opposite an outdoor space 70d or in relation to oneself in the outer space 70d located air.

A lower end 148d the piston rod 90d carries a separating unit 22d that the two chambers 14d and 16d of the syringe plunger 32d separates each other. Furthermore, the piston rod extends 90d in the axial direction 56d from the separating unit 22d through the chamber 16d over the opening 138d out through the cavity 146d up to a cover plate 150d the syringe barrel 142d , The separating unit 22d has separating agent 24d . 26d on that as a filter 28d . 30d are formed and each together with a barrier agent 76d . 78d in a component unit 72d . 74d are arranged. The component unit 74d is fixed to the piston rod via a mechanism not specified here 90d connected. The piston rod 90d passes through the component units 72d and 74d axial and forms at the side 136d a support flange 152d for the component unit 72d that have a spacer element 88d freely movable or rotatable relative to the piston rod 90d is executed. Furthermore, the component units 72d . 74d each a connecting element 80d . 82d on, about which the separating agent 24d . 26d and the barrier means 76d . 78d each connected. The connecting element 80d the component unit 72d serves to cause static friction between the component unit 72d and a surface 84d an interior wall 86d of the syringe plunger 32d to create. By the arrangement of the spacer element 88d or a fixation of the component unit 72d by static friction and / or by the firm connection of the component unit 74d with the piston rod 90d can the separating agents 24d . 26d twisted against each other or opened and closed.

Furthermore, on the cover plate 150d a coupling device 98d arranged with a closure element 102d is closed. The coupling device 98d also has a shaft 154d on, with a corresponding entrance opening 156d at the top 176d interacts.

In addition, the blood sampling device 240 shear body 36d on that in the chamber 14d of the syringe plunger 32d are arranged. The shear bodies 36d Take about a volume of 10 ml of the 60 ml volume of the syringe plunger 32d one.

The following is an application of the blood sampling device 10d described. In 9 is the situation during a blood draw from a patient's vein using a cannula 48d shown. After the blood collection, the cannula becomes 48d removed and the syringe cone 44d is done by unscrewing the protection device 108d locked. The blood is in the chamber 16d of the syringe plunger 32d and is determined by the arrangement of elements ( 24d . 26d . 28d . 30d . 76d . 78d ) of the separating unit 22d limited to these. The barrier agent 76d . 78d prevent blood from passing through the separating means 24d . 26d or the filters 28d . 30d , After taking the blood, the collected blood will be in the blood collection device 10d incubated for 2 to 8 hours at 37 ° C in an incubator.

A situation after a centrifugation of the blood sampling device 10d is in 10 shown. There are three layers formed. At a splash bottom 110d first have the heavy shear body 36d discontinued, followed by the cellular blood components 18d or the blood cake, and lastly, the liquid blood components 20d , the blood serum, accumulated.

To a separation of the blood serum and the liquid blood components 20d from the solid, cellular blood components 18d and the shear bodies 36d becomes the piston rod 90d over the syringe barrel 142d 180 ° in the circumferential direction 112d turned (see 11 ). Due to the fixed arrangement of the piston rod 90d to the component unit 74d at the same time becomes the component unit 74d turned. The component unit 72d remains due to the stiction between the connecting element 80d the component unit 72d and the surface 84d the inner wall 86d of the syringe plunger 32d in their position. By the rotation of the component units 72d and 74d relative to each other, the separating agents 24d . 26d or the filters 28d . 30d the component units 72d . 74d arranged in cover to each other. Furthermore, there is the coupling device 98d after rotation with respect to the piston rod 90d on the other side of the syringe barrel 142d , Now the syringe plunger 32d again in the direction of the splash bottom 110d displaces and thereby reduces the volume of the chamber 14d of the syringe plunger 32d , the liquid blood components can 20d the filters 28d . 30d pass and the blood serum passes, simplified by a negative pressure in the chamber 16d prevails, in the chamber 16d of the syringe plunger 32d , The cellular or solid blood components 18d and the shear bodies 36d be from the filters 28d . 30d retained and remain in the chamber 14d ,

After a complete separation of the liquid blood components 20d becomes the component unit 74d by turning the syringe barrel 142d closed again. At the same time the coupling device 98d back to the original side of the top cylinder 142d rotated, causing the shaft 154d with the entrance opening 156 is aligned and with the chamber 16d can interact to a fluid decrease. In 12 now a sampling is shown. This is the serum through an opening 114d the coupling device 98d taken. A sample syringe 116d takes the place of a closure element 102d to a cone of the coupling device 98b screwed. Will now be a piston 120d the sample syringe 116d in the axial direction 56d withdrawn, can the liquid blood components 20d out of the chamber 16d of the syringe plunger 32d be removed in portions.

13 shows an alternative blood sampling device 10e with a container device 12e that have two chambers 14e . 16e for the separation of blood components 18e . 20e having. The blood sampling device 10e is from a syringe jacket 158e formed by a partition 160e in the two chambers 14e and 16e is divided. The chambers 14e and 16e hold a capacity of 60 ml, the chamber 16e can hold at least a liquid volume of 50 to 55 ml. At a splash bottom 110e is in the area of the chamber 14e a top bonus 44e for receiving a cannula 48e molded and in the area of the chamber 16e there is a coupling device 98e that with a closure element 102e is closed. In every chamber 14e . 16e each extends from a handle element 96e out through an opening 162e in a cover plate 150e of the syringe coat 158e a piston rod 90e . 164e in an axial direction 56e to the splash bottom 110e out. The piston rods 90e . 164e of the chambers 14e . 16e end at a lower end 166e each in a stamp 168e . 170e , Between the chambers 14e . 16e is a separation unit 22e or a separating agent 24e in the form of a semipermeable filter 172e arranged. Furthermore are located in the chamber 14e shear body 36e , which occupy a volume of 10 ml.

When taking a blood sample with the cannula 48e From a patient's vein, the blood is withdrawn by retracting the piston rod 90e the chamber 14e in the chamber 14e moved in. After the blood collection, the cannula becomes 48e removed and the syringe cone 44e is done by unscrewing a guard 108e locked. The blood gets in the chamber 14e held, as the separating unit 22e or the filter 172e through the stamp 170e is closed. Is the chamber 14e with a volume of 50 ml of blood and about 10 ml of shear body 36e filled, pushes a thread 174e the piston rod 90e the chamber 14e to the opening 162e , The Stamp 168e can be there at the opening 162e be attached and the piston rod 90e can from the thread 174e be separated. After taking the blood, the collected blood will be in the blood collection device 10d incubated for 2 to 8 hours at 37 ° C in an incubator.

Subsequently, the blood collection device 10e centrifuged. There are three layers formed. At the splash bottom 110e have the heavy shear body 36e and the cellular blood components 18e deposed, followed by the liquid blood components 20e ,

For a separation of the liquid blood components 20e from the solid, cellular blood components 18e and the shear bodies 36e becomes the piston rod 90e the chamber 14e back in the thread 174e screwed in and the piston rod 90e goes in the direction of the splash bottom 110e shifted, at the same time the piston rod 164e the chamber 16e in the axial direction 56e in the direction of the cover plate 150e drawn. By the pressure generated and the release of the filter 172e through the moving stamp 170e the piston rod 164e become the liquid blood components 20e through the filter 172e pressed and collected in the chamber 16e , The cellular or solid blood components 18e and the shear bodies 36e however, are from the filter 172e retained and remain in the chamber 14e ,

After a complete separation of the liquid blood components 20e becomes the closure element 102e taken off and the serum can through an opening 114e the coupling device 98e be removed. A sample syringe, not shown here in detail, can be attached to a cone of the coupling device 98b be screwed and by retracting a piston, not shown, the sample syringe, the liquid blood components 20e out of the chamber 16e of the syringe coat 158e taken in portions.

10

The blood collection device

12

container means

14

chamber

16

chamber

18

Blood component

20

Blood component

22

separating unit

24

separating means

26

separating means

28

filter

30

filter

32

syringe plunger

34

syringe barrel

36

shear body

38

Bullet

40

surface

42

The End

44

syringe cone

46

external thread

48

cannula

50

inner thread

52

The End

54

opening

56

axial direction

58

The End

60

sealing element

62

outer wall

64

surface

66

surface

68

inner wall

70

outer space

72

component unit

74

component unit

76

barrier means

78

barrier means

80

connecting element

82

connecting element

84

surface

86

inner wall

88

spacer

90

piston rod

92

The End

94

opening

96

handle element

98

coupling device

100

external thread

102

closure element

104

inner thread

106

inner thread

108

guard

110

syringe reason

112

circumferentially

114

opening

116

sampling syringe

118

inner thread

120

piston

122

stabilizing element

124

distance

126

recess

128

clamping element

130

guard

132

preservative

134

protector

136

page

138

opening

140

The End

142

syringe barrel

144

The End

146

cavity

148

The End

150

cover plate

152

support flange

154

shaft

156

inlet opening

158

syringe casing

160

partition wall

162

opening

164

piston rod

166

The End

168

stamp

170

stamp

172

filter

174

thread

176

The End

α

angle

Claims (16)

Blood sampling device with at least one container device ( 12a -E), characterized in that the container device ( 12a -E) at least two chambers ( 14a . 16a ; 14b . 16b ; 14c . 16c ; 14d . 16d ; 14e . 16e ) for the separation of blood components ( 18a . 20a ; 18b . 20b ; 18c . 20c ; 18d . 20d ; 18e . 20e ) having. Blood sampling device according to claim 1, characterized by a separating unit ( 22a -E) used to separate the blood components ( 18a . 20a ; 18b . 20b ; 18c . 20c ; 18d . 20d ; 18e . 20e ) is provided. Blood sampling device according to claim 1 or 2, characterized in that the separating unit ( 22a D) at least two separating agents ( 24a . 26a ; 24b . 26b ; 24c . 26c ; 24d . 26d ) having. Blood sampling device according to claim 3, characterized in that the separating means ( 24a . 26a ; 24b . 26b ; 24c . 26c ; 24d . 26d ) are executed against each other movable. Blood sampling device according to claim 4, characterized in that the separating means ( 24a . 26a ; 24b . 26b ; 24c . 26c ; 24d . 26d ) are arranged rotatably against each other. Blood sampling device according to one of the preceding claims, characterized in that the separating unit ( 22a -E) is closable and / or openable. Blood sampling device according to claim 3, characterized in that the separating unit ( 22a -E) at least one filter ( 28a . 30a ; 28b . 30b ; 28c . 30c ; 28d . 30d ; 172e ). Blood sampling device according to one of the preceding claims, characterized in that the separating unit ( 22a -E) between the chambers ( 14a . 16a ; 14b . 16b ; 14c . 16c ; 14d . 16d ; 14e . 16e ) is arranged. Blood sampling device according to one of the preceding claims, characterized by a syringe plunger ( 32a -E) containing at least one chamber ( 16a -E) forms. Blood sampling device according to claim 9, characterized by a syringe barrel ( 34a c, 142d ), to which the syringe plunger ( 32a -D) is relatively movable. Blood sampling device according to one of the preceding claims, characterized in that at least one of the chambers ( 14a . 16a ; 14b . 16b ; 14c . 16c ; 14d . 16d ; 14e . 16e ) in at least one operating state has a pressure deviating from an atmospheric pressure. Blood sampling device according to one of the preceding claims, characterized by at least one shear body ( 36a -E), who in at least one of the chambers ( 14a -E) is arranged. Blood sampling device according to claim 12, characterized in that at least one shear body ( 36a -E) of a sphere ( 38a -E) is formed. Blood sampling device according to claim 12, characterized in that at least one shear body ( 36a -E) is made of glass. Blood sampling device according to claim 12, characterized in that the shear body ( 36a -E) a smooth surface ( 40a -E). Blood sampling device according to one of the preceding claims, characterized in that at least one of the chambers ( 14a . 16a ; 14b . 16b ; 14c . 16c ; 14d . 16d ; 14e . 16e ) has a capacity of between 30 and 80 ml.