DE102007011329A1 - Method for covering colored histological preparations, which are arranged on microscope slides, involves applying covering medium and fixing covering glass on microscope slides, which are arranged in microscope slides holder - Google Patents
Method for covering colored histological preparations, which are arranged on microscope slides, involves applying covering medium and fixing covering glass on microscope slides, which are arranged in microscope slides holder Download PDFInfo
- Publication number
- DE102007011329A1 DE102007011329A1 DE102007011329A DE102007011329A DE102007011329A1 DE 102007011329 A1 DE102007011329 A1 DE 102007011329A1 DE 102007011329 A DE102007011329 A DE 102007011329A DE 102007011329 A DE102007011329 A DE 102007011329A DE 102007011329 A1 DE102007011329 A1 DE 102007011329A1
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- DE
- Germany
- Prior art keywords
- slides
- microscope slides
- covering
- slide holder
- slide
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Withdrawn
Links
- 238000002360 preparation method Methods 0.000 title claims abstract description 15
- 238000000034 method Methods 0.000 title claims abstract description 12
- 239000011521 glass Substances 0.000 title abstract 2
- 239000006059 cover glass Substances 0.000 claims description 9
- 239000012120 mounting media Substances 0.000 claims description 9
- 238000003780 insertion Methods 0.000 claims 2
- 230000037431 insertion Effects 0.000 claims 2
- 238000011835 investigation Methods 0.000 claims 1
- 238000004043 dyeing Methods 0.000 description 2
- BUHVIAUBTBOHAG-FOYDDCNASA-N (2r,3r,4s,5r)-2-[6-[[2-(3,5-dimethoxyphenyl)-2-(2-methylphenyl)ethyl]amino]purin-9-yl]-5-(hydroxymethyl)oxolane-3,4-diol Chemical compound COC1=CC(OC)=CC(C(CNC=2C=3N=CN(C=3N=CN=2)[C@H]2[C@@H]([C@H](O)[C@@H](CO)O2)O)C=2C(=CC=CC=2)C)=C1 BUHVIAUBTBOHAG-FOYDDCNASA-N 0.000 description 1
- 238000004040 coloring Methods 0.000 description 1
- 239000003814 drug Substances 0.000 description 1
- 229940079593 drug Drugs 0.000 description 1
- 238000001035 drying Methods 0.000 description 1
- 238000011177 media preparation Methods 0.000 description 1
- 238000005086 pumping Methods 0.000 description 1
- 230000000284 resting effect Effects 0.000 description 1
- 238000005070 sampling Methods 0.000 description 1
- 239000002904 solvent Substances 0.000 description 1
- 238000010186 staining Methods 0.000 description 1
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N1/00—Sampling; Preparing specimens for investigation
- G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
- G01N1/30—Staining; Impregnating ; Fixation; Dehydration; Multistep processes for preparing samples of tissue, cell or nucleic acid material and the like for analysis
- G01N1/31—Apparatus therefor
- G01N1/312—Apparatus therefor for samples mounted on planar substrates
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N1/00—Sampling; Preparing specimens for investigation
- G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
- G01N1/36—Embedding or analogous mounting of samples
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- General Health & Medical Sciences (AREA)
- Physics & Mathematics (AREA)
- Chemical & Material Sciences (AREA)
- Analytical Chemistry (AREA)
- Biochemistry (AREA)
- General Physics & Mathematics (AREA)
- Immunology (AREA)
- Pathology (AREA)
- Biomedical Technology (AREA)
- Molecular Biology (AREA)
- Engineering & Computer Science (AREA)
- Sampling And Sample Adjustment (AREA)
- Investigating Or Analysing Biological Materials (AREA)
Abstract
Description
Die Erfindung betrifft ein Verfahren zum Eindecken von auf Objektträgern angeordneten gefärbten histologischen Präparaten nach dem Oberbegriff des Anspruchs 1.The The invention relates to a method for mounting on slides arranged stained histological specimens according to the preamble of claim 1.
Zur Untersuchung und Archivierung histologischer Präparate werden Gewebeschnitte auf einem Objektträger angeordnet, mehrere Objektträger, deren Gewebeschnitte gleichartig bearbeitet werden sollen, in einen Objektträgerhalter verbracht und anschließend der Objektträgerhalter mit den darin befindlichen Objektträgern und den Gewebeschnitten einer mehrstufigen Bearbeitung in Behandlungs- und Färbebädern unterzogen. Dabei wird der Objektträgerhalter mittels eines mehrdimensional verfahrbaren Manipulators nacheinander in Tröge mit Behandlungs- und Färbemitteln getaucht und wieder entnommen. Die Objektträger mit den Gewebeschnitten sind dabei senkrecht ausgerichtet, damit die Behandlungs- und Färbemittel die Gewebeschnitte benetzen und anschließend wieder abtropfen kann.to Examination and archiving of histological specimens tissue sections are placed on a slide, several slides whose tissue sections are similar to be processed, placed in a slide holder and then the slide holder with the therein slides and the tissue sections of a multi-stage processing in treatment and dyebaths subjected. In this case, the slide holder by means of a multi-dimensionally movable manipulator one after the other in troughs dipped with treatment and coloring and removed again. The slides with the tissue sections are aligned vertically, so that the treating and staining the tissue sections wet and then drain again.
Nach der Bearbeitung in den Behandlungs- und Färbebädern erfolgt noch ein Eindecken der auf den Objektträgern angeordneten gefärbten histologischen Präparate.To processing in the treatment and dye baths takes place still covers the arranged on the slides stained histological preparations.
Bei
einer aus der
Der Erfindung liegt die Aufgabe zugrunde, ein Verfahren zum Eindecken von auf Objektträgern angeordneten gefärbten histologischen Präparaten anzugeben, das ein automatisiertes Eindecken von in einem Objektträgerhalter befindlichen Objektträgern mit Präparaten ermöglicht.Of the Invention is based on the object, a method for covering of stained histological slides mounted on slides Specify preparations that have automated coverslipping of slides located in a slide holder with preparations possible.
Diese Aufgabe wird bei einem Verfahren nach dem Oberbegriff des Anspruchs 1 durch die Merkmale dieses Anspruchs gelöst.These Task is in a method according to the preamble of the claim 1 solved by the features of this claim.
Ausgangsbasis ist ein Objektträgerhalter mit Objektträgern und Präparaten, der gerade eine Behandlungs- und Färbeanlage durchlaufen hat und mittels des Manipulators in eine Entnahmestation verbracht wurde. In dieser Entnahmestation befinden sich die Objektträger senkrecht nebeneinander aufgereiht in Positionen des Objektträgerhalters.base is a slide holder with slides and Preparations that just a treatment and dyeing system has passed through and spent by means of the manipulator in a removal station has been. In this sampling station are the slides perpendicular to each other lined up in positions of the slide holder.
Erfindungsgemäß wird der Objektträgerhalter in eine Position gekippt, in der die Objektträger waagerecht mit den Präparaten auf der Oberseite ausgerichtet werden. In dieser Ausrichtung lassen sich die Objektträger einfach waagerecht z. B. durch einen Stößel aus dem Objektträgerhalter herausschieben. Es wird also keine Klammer oder Zange zum Ergreifen benötigt.According to the invention the slide holder is tilted to a position in which the slides horizontally with the preparations be aligned on the top. In this orientation can be the slides are simply horizontal z. B. by a plunger slide out of the slide holder. So it will be no clamp or pliers needed to grasp.
Weiterhin kann in dieser Ausrichtung durch einen Pumpmechanismus ein Eindeckmittel von oben auf das Präparat als Tropfen oder als Spur aufgebracht werden. Das Eindeckmittel kann anschließend seitlich verlaufen und so das Präparat vollständig benetzen. Dabei können auch Lufteinschlüsse nach oben entweichen.Farther can in this orientation by a pumping mechanism a mounting medium applied from above onto the preparation as a drop or as a trace become. The mounting medium can then extend laterally and completely wet the preparation. there Air pockets can also escape upwards.
Schließlich kann in dieser Ausrichtung auch ein Deckglas von oben aufgesetzt werden, ohne dass dieses anschließend verrutscht.After all can put in this orientation, a cover glass from above without this subsequently slipping.
Schließlich kann nach Durchführung dieser Maßnahmen der Objektträger einfach waagerecht, z. B. ebenfalls durch einen Stößel, in den Objektträgerhalter zurück geschoben werden.After all may after carrying out these actions the slide simply horizontal, z. B. also by a plunger, be pushed back into the slide holder.
Durch diese einfachen Bewegungsvorgänge lassen sich die Verfahrensschritte unkompliziert automatisieren.By These simple movement processes can be the process steps easy to automate.
Gemäß einer Weiterbildung kann das Kippen der Objektträgerhalter, das Aus- und Einschieben der Objektträger sowie das Aufbringen von Eindeckmittel und Deckglas maschinell durchgeführt werden.According to one Continuing can be the tilting of the slide holder, the Extend and insert slides and apply machine-mounted by coverslipper and coverslip become.
Dies ermöglicht eine zügige und gleichartige Behandlung aller Objektträger. Ferner kann durch das maschinelle Aufbringen des Eindeckmittels verhindert werden, dass Laborpersonal Lösungsmitteldämpfen ausgesetzt wird, wie es bei manueller Bearbeitung der Fall wäre.This allows a speedy and similar treatment all slides. Furthermore, by the mechanical application of the mounting medium prevent laboratory personnel from fighting solvent exposed, as it would be the case with manual processing.
Vorzugsweise werden die Objektträger nach dem Aufbringen von Eindeckmittel und Deckglas wieder in die gleiche Position des Objektträgerhalters geschoben, in der sie sich vor dem Ausschieben befanden.Preferably The slides are after the application of Eindeckmittel and cover slip pushed back into the same position of the slide holder, in which they were before pushing out.
Dadurch bleibt nach dem Eindecken die Zuordnung der Präparate auf den Objektträgern im Hinblick auf die Positionen im Objektträgerhalter erhalten. Verwechslungen, wie sei bei manueller Bearbeitung auftreten können, werden so vermieden.Thereby after allocation the allocation of drugs remains the slides with respect to the positions in the slide holder receive. Mistakes, as may occur during manual processing can be avoided.
Gemäß einer bevorzugten Ausgestaltung werden die Objektträger in der Bearbeitungsstation in waagerechter Ausrichtung auf einen Drehteller geschoben und durch schrittweises Drehen des Drehtellers zur Eindeckstation und zur Deckglasstation verbracht.According to one preferred embodiment, the slides are in the Processing station in horizontal orientation on a turntable pushed and by stepwise turning the turntable to the Eindeckstation and spent at the deckglass station.
Die Objektträger werden dabei durch einfache Verfahrschritte mit jeweils nur einem Freiheitsgrad zu den einzelnen Stationen transportiert. Dabei können gleichzeitig mehrere Objektträger auf unterschiedlichen Winkelpositionen auf dem Drehteller lagern und durch Drehen gleichzeitig zur nächsten Bearbeitungsstation befördert werden.The Slides are thereby by simple traversing steps transported to the individual stations with only one degree of freedom. It can simultaneously several slides store on different angles on the turntable and by turning simultaneously to the next processing station to get promoted.
Zum Aus- und Einschieben der Objektträger unterschiedlicher Positionen können der Drehteller und/oder der Objektträgerhalter relativ zueinander vertikal verfahren werden, bis die jeweilige Halteposition des Objektträgerhalters mit der Oberfläche des Drehtellers fluchtet.To the Extend and insert the slides different Positions may include the turntable and / or the slide holder be moved vertically relative to each other until the respective Holding position of the slide holder with the surface of the turntable is aligned.
Der Transfer von und zu Positionen des Objektträgerhalters kann dadurch unabhängig von der Transportbewegung des Drehtellers erfolgen. Beliebige Positionen des Objektträgerhalters lassen sich ansteuern.Of the Transfer from and to slide holder positions can thereby independent of the transport movement of the turntable respectively. Any positions of the slide holder can be controlled.
Nachfolgend wird die Erfindung anhand eines Ausführungsbeispiels erläutert, das in der Zeichnung dargestellt ist. In der Zeichnung zeigen:following the invention will be explained with reference to an embodiment, which is shown in the drawing. In the drawing show:
Der
Drehteller
Eine
erste Station, bei der das Aufnahmefeld
Eine
zweite Station
In
einer vierten Station kann optional eine Trocknung vorgenommen werden,
so dass das Eindeckmittel schneller abbindet. Dargestellt ist im
vierten Aufnahmefeld
ZITATE ENTHALTEN IN DER BESCHREIBUNGQUOTES INCLUDE IN THE DESCRIPTION
Diese Liste der vom Anmelder aufgeführten Dokumente wurde automatisiert erzeugt und ist ausschließlich zur besseren Information des Lesers aufgenommen. Die Liste ist nicht Bestandteil der deutschen Patent- bzw. Gebrauchsmusteranmeldung. Das DPMA übernimmt keinerlei Haftung für etwaige Fehler oder Auslassungen.This list The documents listed by the applicant have been automated generated and is solely for better information recorded by the reader. The list is not part of the German Patent or utility model application. The DPMA takes over no liability for any errors or omissions.
Zitierte PatentliteraturCited patent literature
- - DE 112004000212 T5 [0004] - DE 112004000212 T5 [0004]
Claims (5)
Priority Applications (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
DE102007011329A DE102007011329A1 (en) | 2007-03-08 | 2007-03-08 | Method for covering colored histological preparations, which are arranged on microscope slides, involves applying covering medium and fixing covering glass on microscope slides, which are arranged in microscope slides holder |
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
DE102007011329A DE102007011329A1 (en) | 2007-03-08 | 2007-03-08 | Method for covering colored histological preparations, which are arranged on microscope slides, involves applying covering medium and fixing covering glass on microscope slides, which are arranged in microscope slides holder |
Publications (1)
Publication Number | Publication Date |
---|---|
DE102007011329A1 true DE102007011329A1 (en) | 2008-09-11 |
Family
ID=39677940
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
DE102007011329A Withdrawn DE102007011329A1 (en) | 2007-03-08 | 2007-03-08 | Method for covering colored histological preparations, which are arranged on microscope slides, involves applying covering medium and fixing covering glass on microscope slides, which are arranged in microscope slides holder |
Country Status (1)
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DE (1) | DE102007011329A1 (en) |
Cited By (6)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
DE102011009502A1 (en) * | 2011-01-26 | 2012-07-26 | Leica Biosystems Nussloch Gmbh | Covering module for applying coverslips to slides |
US9052256B2 (en) | 2013-03-15 | 2015-06-09 | Leica Biosystems Nussloch Gmbh | Method for processing and embedding tissue |
US9097629B2 (en) | 2013-03-15 | 2015-08-04 | Leica Biosystems Nussloch Gmbh | Tissue cassette with retractable member |
US9389154B2 (en) | 2013-03-15 | 2016-07-12 | Leica Biosystems Nussloch Gmbh | Tissue cassette with biasing element |
US10092905B2 (en) | 2012-06-22 | 2018-10-09 | Leica Biosystems Nussloch Gmbh | Tissue sample container and methods |
US10201331B2 (en) | 2012-06-22 | 2019-02-12 | Leica Biosystems Nussloch Gmbh | Biopsy tissue sample transport device and method of using thereof |
Citations (16)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US4190472A (en) * | 1977-09-28 | 1980-02-26 | Alex Slonicki | Automated system for the application of coverglasses on histological and cytological slides |
US4428793A (en) * | 1981-08-25 | 1984-01-31 | Meisei Electric Co., Ltd. | Preparation method for a microscopic specimen and a device therefor |
WO1995020176A1 (en) * | 1994-01-25 | 1995-07-27 | Australian Biomedical Corporation Ltd. | Instrument for automatically applying coverslips and method |
DE29514506U1 (en) * | 1994-10-07 | 1995-11-02 | Kärchner-Weinkauf, Hannelore, 97616 Bad Neustadt | Slide coverslipper |
US5580414A (en) * | 1992-12-11 | 1996-12-03 | Ljungmann; Oystein H. | Apparatus for automatic application of cover slips on microscope slides |
WO1997003827A1 (en) * | 1995-07-24 | 1997-02-06 | Innovation Instruments, Inc. | Automated slide staining system |
US5989386A (en) * | 1995-06-15 | 1999-11-23 | Vision Instruments Ltd. | Coverslip pick-up and laydown apparatus |
EP1052497A2 (en) * | 1999-05-11 | 2000-11-15 | Kabushiki Kaisha Tiyoda Seisakusho | Cover glass adhering device |
EP0935773B1 (en) * | 1996-11-01 | 2001-02-21 | Microm International GmbH | Appartus for placing a cover on a histological and biological specimen |
DE10144041A1 (en) * | 2001-09-07 | 2003-03-27 | Leica Mikrosysteme Gmbh Wien | Automatic delivery unit for feeding specimen slides from stainer to cover slipper, has transport device which extends out from transfer position to location beyond cut-out in sidewall |
US6626224B1 (en) * | 1998-12-21 | 2003-09-30 | Torstein Ljungmann | Machine for automatically attaching cover slips to microscope slides |
US20040092024A1 (en) * | 2002-04-26 | 2004-05-13 | Ventana Medical Systems, Inc. | Method and apparatus for automated coverslipping |
DE112004000212T5 (en) | 2003-02-27 | 2006-01-12 | Izvoztchikov, Ilia Borisovitch | Device for covering histological and biological samples |
DE10144042B4 (en) * | 2001-09-07 | 2006-04-13 | Leica Microsystems Nussloch Gmbh | Processing device for dyeing and covering slides |
EP1526369B1 (en) * | 1999-11-22 | 2006-06-14 | Ventana Medical Systems, Inc. | Coverslip and method for applying a coverslip to a glass slide |
DE102005042214A1 (en) * | 2005-09-05 | 2007-03-22 | Leica Microsystems Nussloch Gmbh | Receiving and transfer station for covered slides |
-
2007
- 2007-03-08 DE DE102007011329A patent/DE102007011329A1/en not_active Withdrawn
Patent Citations (16)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US4190472A (en) * | 1977-09-28 | 1980-02-26 | Alex Slonicki | Automated system for the application of coverglasses on histological and cytological slides |
US4428793A (en) * | 1981-08-25 | 1984-01-31 | Meisei Electric Co., Ltd. | Preparation method for a microscopic specimen and a device therefor |
US5580414A (en) * | 1992-12-11 | 1996-12-03 | Ljungmann; Oystein H. | Apparatus for automatic application of cover slips on microscope slides |
WO1995020176A1 (en) * | 1994-01-25 | 1995-07-27 | Australian Biomedical Corporation Ltd. | Instrument for automatically applying coverslips and method |
DE29514506U1 (en) * | 1994-10-07 | 1995-11-02 | Kärchner-Weinkauf, Hannelore, 97616 Bad Neustadt | Slide coverslipper |
US5989386A (en) * | 1995-06-15 | 1999-11-23 | Vision Instruments Ltd. | Coverslip pick-up and laydown apparatus |
WO1997003827A1 (en) * | 1995-07-24 | 1997-02-06 | Innovation Instruments, Inc. | Automated slide staining system |
EP0935773B1 (en) * | 1996-11-01 | 2001-02-21 | Microm International GmbH | Appartus for placing a cover on a histological and biological specimen |
US6626224B1 (en) * | 1998-12-21 | 2003-09-30 | Torstein Ljungmann | Machine for automatically attaching cover slips to microscope slides |
EP1052497A2 (en) * | 1999-05-11 | 2000-11-15 | Kabushiki Kaisha Tiyoda Seisakusho | Cover glass adhering device |
EP1526369B1 (en) * | 1999-11-22 | 2006-06-14 | Ventana Medical Systems, Inc. | Coverslip and method for applying a coverslip to a glass slide |
DE10144041A1 (en) * | 2001-09-07 | 2003-03-27 | Leica Mikrosysteme Gmbh Wien | Automatic delivery unit for feeding specimen slides from stainer to cover slipper, has transport device which extends out from transfer position to location beyond cut-out in sidewall |
DE10144042B4 (en) * | 2001-09-07 | 2006-04-13 | Leica Microsystems Nussloch Gmbh | Processing device for dyeing and covering slides |
US20040092024A1 (en) * | 2002-04-26 | 2004-05-13 | Ventana Medical Systems, Inc. | Method and apparatus for automated coverslipping |
DE112004000212T5 (en) | 2003-02-27 | 2006-01-12 | Izvoztchikov, Ilia Borisovitch | Device for covering histological and biological samples |
DE102005042214A1 (en) * | 2005-09-05 | 2007-03-22 | Leica Microsystems Nussloch Gmbh | Receiving and transfer station for covered slides |
Cited By (10)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
DE102011009502A1 (en) * | 2011-01-26 | 2012-07-26 | Leica Biosystems Nussloch Gmbh | Covering module for applying coverslips to slides |
US9011066B2 (en) | 2011-01-26 | 2015-04-21 | Leica Biosystems Nussloch Gmbh | Coverslipping module for mounting coverslips onto specimen slides |
DE102011009502B4 (en) * | 2011-01-26 | 2017-11-02 | Leica Biosystems Nussloch Gmbh | Covering module for applying coverslips to slides |
US10092905B2 (en) | 2012-06-22 | 2018-10-09 | Leica Biosystems Nussloch Gmbh | Tissue sample container and methods |
US10201331B2 (en) | 2012-06-22 | 2019-02-12 | Leica Biosystems Nussloch Gmbh | Biopsy tissue sample transport device and method of using thereof |
US11241220B2 (en) | 2012-06-22 | 2022-02-08 | Leica Biosystems Nussloch Gmbh | Biopsy tissue sample transport device and method of using thereof |
US9052256B2 (en) | 2013-03-15 | 2015-06-09 | Leica Biosystems Nussloch Gmbh | Method for processing and embedding tissue |
US9097629B2 (en) | 2013-03-15 | 2015-08-04 | Leica Biosystems Nussloch Gmbh | Tissue cassette with retractable member |
US9389154B2 (en) | 2013-03-15 | 2016-07-12 | Leica Biosystems Nussloch Gmbh | Tissue cassette with biasing element |
US10345203B2 (en) | 2013-03-15 | 2019-07-09 | Leica Biosystems Nussloch Gmbh | Tissue cassette with biasing element |
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