DE102006049560A1 - Carrier for use in chemical or biochemical assays comprises immobilization zones with membranes that comprise a layer of immobilization material and are overmolded with a thermoplastic material - Google Patents

Abstract

Carrier for use in chemical or biochemical assays comprises immobilization zones which, on contact with a substance containing one partner (I) of a binding pair, locally immobilizes (I). The immobilization zones comprise membranes that comprise a layer of immobilization material and are overmolded with a thermoplastic material. Independent claims are also included for: (1) assay cartridge for use in a device for chemical or biochemical analysis of a test fluid, comprising a carrier as above and a system of channels through which the (I)-containing substance, the test fluid and/or other fluids are supplied to and/or withdrawn from the carrier; (2) producing a carrier as above by placing the immobilization membranes in an injection mold and overmolding them with the thermoplastic material. ACTIVITY : ACTIVITY - MECHANISM OF ACTION : MECHANISM OF ACTION .

Description

The The invention relates to a carrier element for use in chemical or biochemical analytical methods, comprising at least one immobilization surface, which is an immobilization material which, when in contact with a first binding partner a binding pair-containing substance immobilizes the first binding partner locally, a such a support element containing or forming analysis cartridge and a method for Production of such a support element or such an analysis cartridge.

support elements of the type mentioned above are used in analytical methods, in which an analytical fluid, e.g. human or animal sample liquid, to the presence of certain proteins, antibodies, receptors, nucleic acids etc. is examined. The detection of these compounds is carried out by Contacting with a test substance, which corresponding binding partner for the contains compounds to be analyzed in the analysis fluid, so that a reaction between the analysis fluid and the test fluid is present of the corresponding compounds in the analysis fluid. there the reaction taking place can be visually directly visible or also only after addition of a color conjugate as fluorescence signal be optically detected by means of a suitable read-out unit.

Usually finds the described analysis reaction on the surface of a support element the above-mentioned type, wherein first the test substance on the immobilization surface the carrier element is applied and then applied to the analysis fluid becomes. To the spread of the test substance and the analysis fluid on the immobilization surface to control and run away or run down the substances To avoid is the immobilization surface with an immobilization material provided on which the test substance by covalent or non-covalent Binds binding such that contained in the test substance Binding partner for the Fixed analysis reaction with respect to the plane of the immobilization surface becomes. Such immobilization of the test substance is particular important, if a variety on a Immobilisierungsfläche various test substances are to be applied (dot field), so that a large number of analysis tests are carried out simultaneously in one measurement process can. For example, in this way, a blood sample in a measuring process be tested for various antigens of a pathogen.

When support elements come conventionally glass slides to Use, which are coated with the immobilization material or to which a layer of immobilization material is adhered is. Furthermore, it is known to perform the analysis reactions on so-called "bio-chips" on which the test substance is applied in particular as a dot field and which one for that are designed to be interchangeable in a semi-or fully automatic implementation be used in the analysis process. Since carrier elements in general are intended for single use and thus in relatively high quantity produced or purchased, there is great interest a reduction in manufacturing, transport and disposal costs these support elements. by virtue of the relatively complex production of known support elements of a glass plate and an applied or glued immobilization layer However, the costs associated with such support elements can be not lower arbitrarily. The carrier elements made of glass plates also require special attention during transport as well as at Handling to avoid a break of the often thin platelets.

Furthermore are the glass slides as well as those mentioned Bio-chips with reasonable effort only in simple plate geometry produced. If the carrier element however, deviating from such a plate geometry design features have, such as protrusions, Depressions or other means with which the carrier element, for example for the Use in an analysis device to be prepared, so must the glass plate or the organic chip in addition glued with other components or in a transitional frame be used, what additional Costs caused.

In front This is the object of the present invention support element for use in a chemical or biochemical analytical method to provide one for the used analysis method has appropriate shape and good immobilization properties and which at the same time economically producible and more convenient to handle, as well as an economical method of production such a support element specify.

According to the present invention, this object is achieved by a carrier element for use in chemical or biochemical analytical methods comprising at least one immobilization surface comprising an immobilization material which, upon contact with a substance containing a first binding partner of a binding pair, locally immobilizes the first binding partner, said at least an immobilization surface has an immobilization membrane, wel surface is formed of at least one layer formed from the immobilization material, and wherein the at least one immobilization membrane is back-injected with a thermoplastic material.

According to the invention is thus provided that the support element an immobilization membrane containing the immobilization material has, which is back-injected with thermoplastic. In this way, a support element is made an injection molded part, to which an immobilization membrane cohesively is connected.

It has been shown that with the production of a carrier element according to the invention associated costs usual support elements for several reasons are significantly lower. For one, instead of glass, a plastic be used as a mechanical base, resulting in a saving of raw material costs. To the others may apply an adhesive between the immobilization layer and the substrate and the step of applying or sticking the immobilization material dispensed onto the base be, since according to the invention the plastic molded directly onto the immobilization membrane. Furthermore are the carrier elements according to the invention light and almost unbreakable, thus allowing a more comfortable Handling and reduced transport costs.

By appropriate investigations could be further shown that the carrier elements according to the invention a reliable one Immobilization of the corresponding binding partner of the test substance on the immobilization surface demonstrate. This was not to be expected. immobilisation materials are basically across from mechanical or thermal stress very sensitive and wise usually a fine pore structure for binding binding partners of the test fluid. Such materials are usually only in laboratory environment and handled with the appropriate care. With the manufacturing environment of injection molded parts, which are under an injection pressure of mostly in of the order of magnitude of 500 bar and at temperatures of, for example, about 200 ° C produced Such materials are apparently incompatible in character. To be expected a destruction the pore structure by the squeezing of the immobilization material under the high injection pressure combined with high temperature or but an intrusion of the liquid Plastic in the pore structure and thus clogging the pore structure. Surprised However, it has been found that the immobilization properties the immobilization material in the carrier element according to the invention not or not significantly impaired.

The Shaping the carrier element according to the invention can also be chosen almost freely without significantly increasing production costs, because by suitable shaping of the injection molding tool projections, depressions, etc., e.g. for coupling to a holder of an analysis device, can be sprayed in a simple way. Also more complex support elements and in particular support elements with several separate support surfaces, which each carry a separate immobilization membrane, thus be sprayed in one step.

If in the present description and the claims of a surface, in particular of an immobilization surface, we are talking about not just flat geometries become. The immobilization area can for special For purposes of use also at least one curved, i. not planar Section. So the Immobilisierungsfläche a Trough or camber contain, e.g. the flow behavior from to the immobilization surface to influence applied substances. In a constructive special simple embodiment is the immobilization area however, as a flat surface, i.e. as a planar surface, educated.

In a preferred embodiment the immobilization membrane is a self-supporting membrane. Such support element has particularly low production costs, as a self-supporting membrane convenient to use in the injection mold and after cooling the plastic along with the plastic from the injection mold removed is.

In a further preferred embodiment the immobilization membrane further has a stabilizing layer on, in particular a stabilizing layer, which has a higher stability, as the immobilization material layer. Such a stabilization layer improves the mechanical stability of the immobilization membrane, to handle the immobilization membrane during injection molding, so e.g. the mounting of the immobilization membrane in an injection mold, to improve.

The stabilization layer can be arranged on the side of the immobilization material layer facing the plastic and connected thereto. Such an arrangement exhibits a particularly reliable maintenance of the immobilization properties of the immobilization material, since the stabilization layer acts as a barrier between the plastic and the immobilization material layer and prevents direct contact of the liquid plastic with the immobilization material during the injection of the plastic. For Such a stabilizing layer may be advantageously pulp material or vinyl plus copolymer.

alternative or additionally may be a stabilizing layer on the side facing away from the plastic the immobilization membrane be arranged and as a removable layer, in particular as a peelable film, be formed. Especially in combination with a stabilization layer arranged on the plastic side can then be a particularly high stability and protection of Immobilisierungsmaterialschicht be achieved. The arranged on the side facing away from the plastic Stabilization layer leaves then peel off the immobilization material layer before beginning the analysis procedure expose.

In addition, but also independent from the idea of stabilization of the immobilization membrane, It is also thought that a protective layer on the plastic opposite side of the immobilization membrane directly on the Immobilisierungsmaterialschicht is arranged and as removable Layer, in particular as a peelable plastic film formed is. With such a detachable Protective layer may be the sensitive immobilization material layer especially during the injection molding from damage by external mechanical and / or thermal influences are protected, so that despite the during the injection molding used pressure and temperature conditions a very good immobilization property the immobilization material is retained.

ever after composition of the immobilization membrane and according to special Requirements for the durability of the support member may further be provided be that the immobilization membrane facing at its plastic Side a transitional layer which has a higher connectivity to the plastic material than the adjacent layer of Immobilisierungsmembran. Even such a transitional layer can be next to the improvement of the adhesion between the immobilization material and the plastic also take the effect of a barrier to a direct action of the liquid Plastic on other layers of the immobilization membrane during the injection molding to prevent or / and can have a stabilizing effect.

In a further preferred embodiment For example, the immobilization material has a microscopic pore structure on which for a non-covalent Connection of the first binding partner is set up. A non-covalent Connection of the first binding partner is preferred in many analysis methods, there a chemical change the binding partner in the immobilization and thus a possible influence the analysis reaction can be largely excluded.

A particularly effective immobilization by non-covalent attachment for one Variety of binding partners or for specific binding partners can be achieved if the immobilization material is a medium Pore size between approximately 0.05 μm and about 1.0 μm, preferably between about 0.1 μm and approximately 0.7 μm, the strongest preferably about 0.2 μm or about 0.45 μm or about 0.6 μm.

specially can be used as immobilizing nitrocellulose, polyvinyl fluoride (PVDF) or nylon are used, wherein the immobilization material for one particularly effective immobilization preferably completely one of these compounds is formed. Especially in terms of the use of nitrocellulose represent the successful test trials for the production of the carrier elements according to the invention is a special surprise. Nitrocellulose is also known as "shooting cotton" and shows depending on the material condition flammable to explosive properties. A back injection This material with plastic high temperature and high pressure seems therefore in particular for this material is particularly outlandish.

on the other hand However, it is also thought that the immobilization material has at least one functional group which is covalent Connection to the first binding partner is established. Such covalent attachment can lead to a more stable and defined immobilization because of the stronger ones covalent interactions between the immobilization material and the first binding partner. A binding partner immobilized by covalent attachment is also more resistant across from mechanical effects and so is less by other fluids washed off the immobilization surface.

With particular advantage is a support element of the abovementioned type are used in an analysis method, in which the first or / and the second binding partner of the binding pair selected is from: proteins, especially receptors, antibodies, nucleic acids, in particular RNA, DNA, carbohydrates, lipids, there especially for such analysis methods greater Demand for inexpensive and at the same time high quality support elements consists.

In a further preferred embodiment of the invention it is provided that the carrier element has a plurality of immobilization surfaces each having an associated immobilization has membrane, and that the Immobilisierungsflächen are arranged on a common injection molded body. Such a carrier element allows the grouping of a plurality of mutually separated immobilization surfaces on one and the same carrier element in order to examine, for example, an analysis fluid conveniently for the presence of a plurality of different proteins, antibodies, receptors, nucleic acids, etc.

Especially is thought that the injection molded body as an analysis cassette with a grid-like arrangement of separate immobilization surfaces formed is. Such cassettes can about for the manual implementation a plurality of parallel analysis reactions, but advantageous also for automatic processing in automated or semi-automated analyzers be suitable or furnished. To have a reliable separation of the different ones Immobilisierungsflächen Further, to ensure associated analysis responses the immobilization surfaces respectively surrounded by uniform material molded with the injection molded partitions be.

To In another aspect of the present invention, the above-mentioned inventive task solved by a carrier element for use in chemical and biochemical analytical methods, comprising at least one immobilization surface comprising an immobilization material, when in contact with a first binding partner of a binding pair containing substance immobilizes the first binding partner locally, wherein the at least one immobilization surface is an immobilization membrane comprising at least one of the immobilization material is formed, and wherein the carrier element further comprising a channel assembly which communicates with the immobilization membrane is connected and can be connected to a vacuum source, around on one of the exposed side of the immobilization surface opposite Side of the real estate to generate a negative pressure.

By the generated in the manner of the invention Negative pressure is a test substance applied to the immobilization surface aspirated to the immobilization membrane and in particular in the certain dimensions sucked into the Immobilisierungsmaterialschicht inside, thereby special in the case of a non-covalent attachment of the binding partner one even more reliable Connection and thus achieved an even more stable immobilization becomes. A certain suction power of the negative pressure source in cooperation with the channel assembly and the immobilization membrane then performs to a certain immobilization capacity or a certain immobilization speed, so that in particular by changing the suction power a Adaptation of the carrier element can be made possible to existing immobilization tasks.

In a preferred embodiment has the carrier element a base body on which the immobilization membrane is arranged, wherein the channel arrangement in the base body is trained. The base body can then on the one hand as an investment and support body for the Immobilisierungsmembran and on the other hand as a carrier for the Channel arrangement serve.

To a further preferred embodiment The invention relates to the channel arrangement in a carrier element of the above-described Art provided in the injected plastic, stands with the Immobilizing membrane in conjunction and is also connected to a Vacuum source can be connected, around on a plastic facing side of the immobilization membrane to generate a negative pressure. Such a channel arrangement can be in particular in one according to the present invention Invention produced by an injection molding carrier element realize particularly easily, since the channel arrangement directly during the injection molding formed by appropriate shaping of the injection molding tool can be and thus a subsequent machining omitted the carrier element can be or at least significantly simplified.

In a particularly simple embodiment a support element with channel arrangement is provided that the plastic in the form of a Grid structure, a screen structure or a substantially plate-shaped base body with at least one passage opening sprayed. Especially when using a grid structure or a sieve structure then also a relatively even distribution of negative pressure over the immobilization surface achieve.

To achieve the above object, the invention further provides an analysis cartridge for use in an analysis apparatus for chemical or biochemical analysis of an analysis fluid, in particular an animal or human sample liquid, wherein the cartridge comprises or forms a support member of the type described above, and wherein the cartridge has a channel system for directing and / or removing the substance containing the first binding partner, the analysis fluid, or / and optionally further fluids to the immobilization surface of the carrier element. Such an analysis cartridge can be designed as a disposable cartridge and, in combination with a corresponding analysis device, provides a ready-to-use Ka nalanordnung for supply and discharge of the substances necessary for the analysis process.

The analysis cartridge can also be inexpensively manufactured from an injection molding material and can with regard to the formation of the channel system at one in the DE 10 2005 002 990 A1 oriented analysis cartridge. It may for example have a plate-like shape, with a receptacle for holding the support element. For easy mounting and secure positioning of the support element on the analysis cartridge corresponding mounting means are provided on the analysis cartridge, eg step sections, projections or depressions, etc., which engage with the support member or with complementary mounting means of the support member during assembly of the support member. In addition, misoperation control means may be required on the support member to securely prevent incorrect insertion of the support member. By equipping analysis cartridges with carrier elements of the invention, the costs associated with the analysis can be further reduced. The thermoplastic material can be injection molded cost-effectively in the exact desired shape and also the complementary mounting means can be easily formed in the same process step.

If the analysis cartridge also has a molded component of thermoplastic, for example as in the DE 10 2005 002 990 A1 described comprises a plastic plate produced by an injection molding process, in which also parts of the channel system are formed, it can also be thought in a particularly simple embodiment, to inject this plastic part directly in its preparation to an immobilizing membrane. The carrier element is then an integral part of the analysis cartridge or forms the analysis cartridge.

to solution In the above-mentioned object, the invention further provides a method for producing a carrier element having at least one immobilization surface ready, wherein the at least one immobilization surface an immobilizing material which, when in contact with a a first binding partner of a binding pair-containing substance the first binding partner locally immobilized, comprising the following steps: insertion at least a cantilevered immobilization membrane, which consists of at least a built up of the immobilization material layer is, in an injection mold, and back injection of at least an immobilization membrane with a thermoplastic. As before already described, can be with such a method carrier elements very economical in big quantity produce and special wishes in terms of the shape of the support member and the number and arrangement of Immobilisierungsflächen can easily through appropriate adaptation of the injection molding tool to be met. Immobilization ability of the Immobilization material is not or not essential impaired.

Preferably becomes the at least one immobilization membrane after insertion in the injection mold and at least before the back injection of a membrane holding device held in position. This poses a trouble-free Procedure of the injection molding process and a consistently reliable quality of the produced support elements for sure. In particular, if the at least one immobilization membrane back-injected in the injection molding tool in a non-horizontal position is, can slip through the membrane holding device or Falling out of the Immobilisierungsmembran, for example, during the closing of a Mold, to be prevented.

In a simple realization of such a membrane holding device this has at least one undercut in a mold half of the injection molding tool and the at least one immobilization membrane is inserted in the mold half positioned with its edge portion behind the undercut.

The Invention will be described below with reference to preferred embodiments explained in more detail with reference to the drawings.

1 shows a perspective view of a first embodiment of a carrier element according to the invention.

2 shows a cross-sectional view of the in 1 illustrated carrier element.

3 to 5 show cross-sectional views of a second, third and fourth embodiment of the invention.

6 shows a perspective view of the in 5 shown carrier element.

7 shows a perspective view of an analysis cartridge for receiving a support member according to a fifth embodiment of the invention.

8th shows a cross-sectional view of the carrier element for use in the in 7 shown analysis cartridge.

In 1 is a support member according to a first embodiment of the invention in general With 10 designated. The carrier element 10 has an approximately plate-like shape with a rectangular base, and includes a plastic base 12 , which is produced by injection molding. The plastic base 12 has a grid-like arrangement of rectangular depressions 13 on, in the embodiment of 1 two lines with five wells each 13 , The wells 13 are separated from each other by partitions 13a separated and at the edge of the support member by boundary walls 13b limited.

On a floor surface 13c each of the wells 13 is in each case an immobilization membrane 14 arranged, which will be described later in more detail. The floor area 13c and thus also the immobilization membrane 14 are all substantially in the same plane, which is parallel to the plane of the plate-shaped support member 10 runs.

The partitions 13a and the boundary walls 13b the wells 13 are an integral part of the base 12 formed as a one-piece plastic injection-molded body and they are substantially perpendicular to the plane of the support member 10 ,

The following will be with reference to 2 to 4 Variants according to the invention for the construction of the immobilization membranes 14 explained. It should be noted here that in all the figures showing the immobilization membrane the individual layers are shown with greatly increased thickness for illustrative reasons.

In 2 is the base 12 , which is a plastic body, on the immobilization membranes 14 molded. That is, the immobilization membranes 14 were used to make the carrier element 10 used in a mold half of a mold and by the subsequent injection molding process on the one hand the basis 12 molded and on the other hand, a secure cohesive connection between the base 12 and the immobilization membranes 14 created.

The immobilization membranes 14 , In the embodiment shown, consist of substantially pure nitrocellulose and have a thickness of the order of about one tenth of a millimeter, for example about 140 microns. The nitrocellulose used is formed as a microporous membrane with a pore size of about 0.45 microns, at which a variety of different binding partners, which are used in chemical or biochemical analysis methods can be reliably immobilized by non-covalent attachment.

In 3 is a second embodiment of a carrier element according to the invention generally with 110 designated. It corresponds in many respects to the carrier element 10 , The first embodiment, so that in the following, only the differences from the first embodiment will be discussed in more detail.

In the carrier element 110 according to the second embodiment, the immobilization membranes 114 each of a layer arrangement of a pulp layer 124 and a nitrocellulose layer 126 educated. The immobilization membranes 114 are thus much thicker and thus more stable. Their thickness is in the range of half a millimeter, for example about 560 microns, so that these immobilization membranes can be handled very well during manufacture. In addition, the sensitive nitrocellulose is largely protected from direct contact with liquid plastic by the arrangement of the pulp between the plastic and the nitrocellulose layer.

4 shows a third embodiment of the invention. A carrier element 210 according to the third embodiment corresponds in many features to the embodiments described above, so that will be discussed in more detail below only the differences.

In the third embodiment, the immobilization membranes are 214 from a layer of vinyl and copolymer 224 and a nitrocellulose layer 226 constructed, with the layer of vinyl and copolymer 224 the base 212 the carrier element 210 facing and the nitrocellulose layer 226 is exposed to form the Immobilisierungsfläche. Through the layer of vinyl and copolymer 224 is in the carrier element 210 achieved an improved cohesive connection to the plastic material during back injection. The layer of vinyl and copolymer 224 thus acts as a transitional layer between the base 212 and the nitrocellulose layer 226 ,

5 and 6 show a carrier element 310 according to a fourth embodiment of the invention in a sectional view or in perspective view. The carrier element 310 This embodiment corresponds in many features to the previously described embodiments, so that in the following, only the differences will be discussed in more detail.

In the carrier element 310 are in the base 312 a plurality of passageways 328 formed, which the support element 310 completely penetrate substantially perpendicular to the plane of the plate. In particular, the channels 328 under each of the immobilization membranes 314 arranged so that the immobilization membranes 314 openings 330 of the channels 328 cover.

The channels 328 can have any conceivable arrangement, arrangement density, cross-sectional area, etc., in particular, the channels 328 below the immobilization membranes 314 form a grid structure or a screen structure.

The of the immobilization membranes 314 remote openings 332 of the channels 328 can be connected to a vacuum source which releases air from the channels 328 sucks so that in the channels 328 a negative pressure is generated. This negative pressure will be due in part to the somewhat air-permeable immobilization membranes 314 continued and thus allows an aspiration of the immobilization membranes 314 applied test substance to further improve the immobilization.

The immobilization membranes 314 of the third embodiment are made of a nitrocellulose layer 326 and a peelable protective film disposed thereon 334 formed, being the base 312 directly at the nitrocellulose layer 326 is sprayed and the protective film 334 on the side facing away from the plastic of the nitrocellulose layer 326 is arranged. Through the protective film 334 Can the delicate nitrocellulose layer 326 during the manufacture of the carrier element 310 be protected against external influences, such as scratching, contamination or the like.

The in the illustrated embodiments according to 2 to 5 Layer structures proposed for the immobilization membranes are selected only by way of example and, if desired, can be combined in a variety of ways.

7 shows an analysis cartridge 400 into which a in 8th illustrated inventive carrier element 410 is used. The analysis cartridge 400 has a base plate 436 and one over a surface side of the base plate 436 extending film 438 on. In the slide 438 facing surface side of the base plate 436 are longitudinal grooves 440 molded, which from the over the base plate 436 drawn foil 438 be covered so that in the interface between base plate 436 and foil 438 running channels 442 be formed. The channels 442 connect one with a stopper 444 coverable sample inlet 446 , Fluid inlets / outlets 448 for introducing and discharging flushing fluid and / or analysis fluid and / or contrast agents, etc., as well as the carrier element 410 together in a predetermined manner, so that successively required for the implementation of the analysis method fluids to the immobilization surface of the support element 410 be conducted and, if desired, cleaning operations of the channel system can take place.

The analysis cartridge 400 has for receiving the carrier element 410 a central recess 450 on. For mounting the carrier element 410 at the recess 450 includes the carrier element 410 one the immobilization membrane 414 circumferential paragraph 416 , a the carrier element 410 circumferential annular projection 418 as well as a positioning projection 420 on. At the foot of the paragraph 416 is also a circumferential bead 422 formed, which the seal to one around the paragraph 416 around to be placed O-ring 423 improved. In the carrier element according to the invention, those deviating from a pure plate shape features, ie the paragraph 416 , the ring protrusion 418 , the positioning tab 420 as well as the circumferential bead 422 be molded in one piece without significant overhead by appropriate design of the injection mold.

The carrier element 410 is in the central recess 450 the cartouche 400 used so that the support element 410 with his paragraph 416 or the immobilization membrane 414 circumferential portion at a circumferential edge of the recess 450 supports and the O-ring 423 a seal between the carrier element 410 and the edge of the recess 450 supported.

The analysis cartridge 400 , in particular the base plate 436 is preferably the same as the carrier element 410 formed in an injection molding process of thermoplastic material, so that both components are very economical to produce in large quantities.

The Support elements according to the invention as well as the analysis cartridge according to the invention are particularly suitable for the analysis of human or animal body fluid, such as serum, Blood, plasma, urine etc., on the content of certain proteins, nucleic acids etc. Here comes an analysis reaction between two binding partners a binding pair are used, of which a first binding partner in the test substance to be immobilized on the immobilization surface of the carrier element and the other binding partner potentially in the analysis sample of body fluid is included. examples for such binding pairs are: antigen-antibody, protein-receptor, nucleic acid-nucleic acid (e.g. DNA-DNA, DNA-RNA, RNA-RNA) as well as protein-nucleic acid.

Claims (23)

Carrier element ( 10 ; 110 ; 210 ; 310 ; 410 ) for use in chemical or biochemical analytical methods, comprising at least one Im mobilization surface, which is an immobilization material ( 14 ; 126 ; 226 ; 326 ) which locally immobilizes the first binding partner on contact with a substance containing a first binding partner of a binding pair, characterized in that the at least one immobilization surface comprises an immobilization membrane ( 14 ; 114 ; 214 ; 314 ), which consists of at least one of the immobilization material ( 14 ; 126 ; 226 ; 326 ) and that the immobilization membrane ( 14 ; 114 ; 214 ; 314 ) with a thermoplastic material ( 12 ; 112 ; 212 ; 312 ) is injected behind. Carrier element ( 10 ; 110 ; 210 ; 310 ; 410 ) according to claim 1, characterized in that the immobilization membrane ( 14 ; 114 ; 214 ; 314 ) is a self-supporting membrane. Carrier element ( 110 ; 210 ; 310 ; 410 ) according to claim 1 or claim 2, characterized in that the immobilization membrane further comprises at least one stabilizing layer ( 124 ; 224 ; 324 ), in particular a stabilization layer which has a higher stability than the immobilization material layer (US Pat. 126 ; 226 ; 326 ). Carrier element ( 110 ; 210 ) according to claim 3, characterized in that the stabilizing layer ( 124 ; 224 ) on the plastic ( 112 ; 212 ) facing side of the immobilization material layer ( 126 ; 226 ) is arranged and connected to it and is preferably formed of cellulose material or of vinyl plus copolymer. Carrier element ( 310 ) according to claim 3 or claim 4, characterized in that the stabilizing layer ( 334 ) on the plastic ( 312 ) facing away from the immobilization membrane ( 314 ) and is designed as a removable layer, in particular as a peelable film. Carrier element ( 310 ) according to one of the preceding claims, characterized in that a protective layer ( 334 ) on the side facing away from the plastic of the immobilization membrane ( 314 ) directly on the immobilization material layer ( 326 ) and is designed as a removable layer, in particular as a peelable plastic film. Carrier element ( 210 ) according to one of the preceding claims, characterized in that the immobilization membrane ( 214 ) on her the plastic ( 212 ) facing side a transition layer ( 224 ), which has a higher ability to bond to the plastic material than the adjacent layer ( 226 ) of the immobilization membrane ( 214 ). Carrier element ( 10 ; 110 ; 210 ; 310 ) according to one of the preceding claims, characterized in that the immobilization material ( 14 ; 126 ; 226 ; 326 ) has a microscopic pore structure which is adapted for noncovalent attachment of the first binding partner. Carrier element ( 10 ; 110 ; 210 ; 310 ) according to one of the preceding claims, characterized in that the immobilization material ( 14 ; 126 ; 226 ; 326 ) has an average pore size between about 0.05 μm and about 1.0 μm, preferably between about 0.1 μm and about 0.7 μm, most preferably about 0.2 μm or about 0.45 μm or about 0.6 μm. Carrier element ( 10 ; 110 ; 210 ; 310 ) according to one of the preceding claims, characterized in that the immobilization material ( 14 ; 126 ; 226 ; 326 ) Comprises nitrocellulose, polyvinylidene fluoride (PVDF) or nylon and is preferably formed substantially entirely from one of these compounds. support element according to one of the preceding claims, characterized the immobilization material is at least one functional group which has for a covalent attachment to the first binding partner is established. Carrier element ( 10 ; 110 ; 210 ; 310 ) according to one of the preceding claims, characterized in that the first and / or the second binding partner of the binding pair is selected from: proteins, in particular receptors, antibodies, nucleic acids, in particular RNA, DNA, carbohydrates, lipids. Carrier element ( 10 ; 110 ; 210 ; 310 ) according to one of the preceding claims, characterized in that the carrier element ( 10 ; 110 ; 210 ; 310 ) a plurality of immobilization surfaces ( 13c ) each having an associated immobilization membrane ( 14 ; 114 ; 214 ; 314 ) and that the immobilization surfaces ( 13c ) on a common injection-molded body ( 12 ; 112 ; 212 ; 312 ) are arranged. Carrier element ( 10 ; 110 ; 210 ; 310 ) according to claim 13, characterized in that the injection-molded body as an analysis cassette with a grid-like arrangement of separate immobilization surfaces ( 13c ; 313c ) is trained. Carrier element ( 10 ; 110 ; 210 ; 310 ) according to claim 13 or claim 14, characterized in that the immobilization surfaces ( 13c ; 313c ) each of uniform material with the injection molded body partitions ( 13a ; 13b ) surround are. Carrier element ( 310 ) according to the preamble of claim 1, characterized in that the at least one immobilization surface ( 313c ) an immobilization membrane ( 314 ), which is constructed from at least one layer formed from the immobilization material, and that the carrier element further comprises a channel arrangement ( 328 ), which with the immobilization membrane ( 314 ) is connected and can be connected to a vacuum source in order to generate a negative pressure on one of the exposed side of the immobilization surface opposite side of the Immobilisierungsfläche. Carrier element ( 310 ) according to claim 16, further characterized by a base body ( 312 ), to which the immobilization membrane ( 314 ), the channel arrangement ( 328 ) in the base body ( 312 ) is trained. Carrier element ( 310 ) according to one of claims 1 to 12, characterized in that the channel arrangement ( 328 ) in the back-injected plastic ( 312 ) formed with the immobilization membrane ( 314 ) and is connectable to a source of negative pressure in order to act on a plastic ( 312 ) facing side of the immobilization membrane ( 314 ) to generate a negative pressure. Carrier element ( 310 ) according to one of the preceding claims, characterized in that the plastic ( 312 ) or the base body in the form of a grid structure, a screen structure or a substantially plate-shaped base body is sprayed with at least one passage opening. Analysis cartridge ( 400 ) for use in an analytical device for the chemical or biochemical analysis of an analysis fluid, in particular an animal or human sample fluid, the cartridge ( 400 ) a carrier element ( 410 ) according to one of the preceding claims, and wherein the cartridge ( 400 ) a channel system ( 442 ; 446 ; 448 ), to the substance containing the first binding partner, the analysis fluid and / or optionally further fluids to the immobilization surface of the carrier element ( 400 ) and / or dissipate them. Method for producing a carrier element ( 10 ; 110 ; 210 ; 310 ; 410 ) having at least one immobilization surface, wherein the at least one immobilization surface is an immobilization material ( 14 ; 126 ; 226 ; 326 ), which immobilizes the first binding partner locally upon contact with a substance containing a first binding partner of a binding pair, in particular a method for producing a carrier element according to one of claims 1 to 19 or an analysis cartridge according to claim 20, characterized by the following steps: inserting at least one Immobilization membrane ( 14 ; 114 ; 214 ; 314 ), which consists of at least one of the immobilization material ( 14 ; 126 ; 226 ; 326 ) is formed in an injection mold, and injection molding the at least one immobilization membrane ( 14 ; 114 ; 214 ; 314 ) with a thermoplastic material. A method according to claim 21, characterized in that the at least one immobilization membrane ( 14 ; 114 ; 214 ; 314 ) is held in position after insertion into the injection mold and at least prior to injection from a membrane holding device. A method according to claim 22, characterized in that the at least one immobilization membrane ( 14 ; 114 ; 214 ; 314 ) is inserted into a mold half of the injection molding tool, wherein the mold half has at least one undercut as a membrane holding device, behind which an edge portion of the immobilization membrane ( 14 ; 114 ; 214 ; 314 ) is positioned.