DE10145043A1 - Process and manufacture of fine chemicals - Google Patents

Process and manufacture of fine chemicals

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Publication number
DE10145043A1
DE10145043A1 DE10145043A DE10145043A DE10145043A1 DE 10145043 A1 DE10145043 A1 DE 10145043A1 DE 10145043 A DE10145043 A DE 10145043A DE 10145043 A DE10145043 A DE 10145043A DE 10145043 A1 DE10145043 A1 DE 10145043A1
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Germany
Prior art keywords
dehydrogenase
synthase
transferase
protein
phosphate
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DE10145043A
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German (de)
Inventor
Thomas Hermann
Brigitte Bathe
Mechthild Rieping
Daniela Kruse
Eberhard Busker
Cornel Venzageo
Mike Farwick
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Evonik Operations GmbH
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Degussa GmbH
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Priority to DE10145043A priority Critical patent/DE10145043A1/en
Priority to PCT/EP2002/010174 priority patent/WO2003023016A2/en
Priority to AU2002362280A priority patent/AU2002362280A1/en
Priority to EP02797981A priority patent/EP1425424A2/en
Publication of DE10145043A1 publication Critical patent/DE10145043A1/en
Withdrawn legal-status Critical Current

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/93—Ligases (6)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004—Oxidoreductases (1.)
    • C12N9/0006—Oxidoreductases (1.) acting on CH-OH groups as donors (1.1)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/88—Lyases (4.)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P13/00—Preparation of nitrogen-containing organic compounds
    • C12P13/04—Alpha- or beta- amino acids
    • C12P13/08—Lysine; Diaminopimelic acid; Threonine; Valine

Abstract

Gegenstand der Erfindung sind genetisch veränderte Mikroorganismen, insbesondere coryneforme Bakterien und Escherichia coli, in denen Proteine oder Polypeptide in ihrem Phophorylierungszustand so geändert sind, daß die Bakterien eine größere Menge einer gewünschten Feinchemikalie oder eines Metaboliten als der Wildtyp herstellen, sowie ein Verfahren zur Herstellung von Feinchemikalien oder Metaboliten.The invention relates to genetically modified microorganisms, in particular coryneform bacteria and Escherichia coli, in which proteins or polypeptides are changed in their phosphorylation state so that the bacteria produce a larger amount of a desired fine chemical or metabolite than the wild type, and a process for the preparation of Fine chemicals or metabolites.

Description

Gegenstand der Erfindung sind gentechnisch veränderte Mikroorganismen, insbesondere coryneforme Bakterien und Escherichia coli, in denen Proteine oder Polypeptide in ihrem Phosphorylierungszustand so geändert sind, daß die Bakterien eine größere Menge einer gewünschten Feinchemikalie oder eines Metaboliten als der Wildtyp herstellen, sowie ein Verfahren zur Herstellung von Feinchemikalien oder Metaboliten. The invention relates to genetically modified Microorganisms, especially coryneform bacteria and Escherichia coli, in which proteins or polypeptides are found in their phosphorylation state are changed so that the Bacteria a larger amount of a desired one Fine chemical or a metabolite as the wild type produce, as well as a method for the production of Fine chemicals or metabolites.

Der Bedarf an Feinchemikalien für verschiedenste Anwendungsbereiche ist in den letzten Jahren deutlich gestiegen und die Anforderungen an die Qualität und Reinheit der Substanzen sind sehr hoch. Z. B. finden Aminosäuren und Vitamine in der Humanmedizin, in der pharmazeutischen Industrie, in der Lebensmittelindustrie, insbesondere aber in der Tierernährung Anwendung. Es besteht daher ein allgemeines Interesse daran, neue verbesserte Verfahren zur Herstellung von Aminosäuren, Vitaminen oder anderen Feinchemikalien bereitzustellen. The need for fine chemicals for various Areas of application has been clear in recent years increased and the requirements for quality and The purity of the substances is very high. For example, find Amino acids and vitamins in human medicine, in pharmaceutical industry, in the food industry, but especially in animal nutrition. It there is therefore a general interest in creating new ones improved processes for the production of amino acids, Provide vitamins or other fine chemicals.

Es ist bekannt, daß Aminosäuren und Vitamine durch Fermentation von Stämmen coryneformer Bakterien, insbesondere Corynebacterium glutamicum, Enterobacteriaceae, insbesondere Escherichia coli oder mit Bacilli, insbesondere Bacillus subtilis hergestellt werden. Daneben werden eine Vielzahl anderer Bakterien oder Pilze genutzt, um Feinchemikalien oder Metaboliten zu produzieren. Wegen der großen Bedeutung wird ständig an der Verbesserung der Herstellverfahren gearbeitet. Verfahrensverbesserungen können fermentationstechnische Maßnahmen wie z. B. Rühren und Versorgung mit Sauerstoff, oder die Zusammensetzung der Nährmedien wie z. B. die Zuckerkonzentration während der Fermentation, oder die Aufarbeitung zur Produktform durch z. B. Ionenaustauschchromatographie oder die intrinsischen Leistungseigenschaften des Mikroorganismus selbst betreffen. It is known that through amino acids and vitamins Fermentation of strains of coryneform bacteria, especially Corynebacterium glutamicum, Enterobacteriaceae, especially Escherichia coli or with Bacilli, especially Bacillus subtilis. In addition, a variety of other bacteria or fungi used to fine chemicals or metabolites to produce. Because of the great importance is constantly on the Improved manufacturing processes worked. Process improvements can result from fermentation technology Measures such as B. stirring and supply of oxygen, or the composition of the nutrient media such. B. the Sugar concentration during fermentation, or the Refurbishment to the product form by e.g. B. Ion exchange chromatography or the intrinsic Performance characteristics of the microorganism itself affect.

Zur Verbesserung der Leistungseigenschaften dieser Mikroorganismen werden Methoden der Mutagenese, Selektion und Mutantenauswahl angewendet. Seit einigen Jahren werden außerdem Methoden der rekombinanten DNA-Technik zur Stammverbesserung von Aminosäure-produzierenden Stämmen von Corynebacterium eingesetzt, indem man einzelne Aminosäure- Biosynthesegene amplifiziert und die Auswirkung auf die Aminosäure-Produktion untersucht. Übersichtsartikel hierzu findet man unter anderem bei Kinoshita ("Glutamic Acid Bacteria", in: Biology of Industrial Microorganisms, Demain and Solomon (Eds.), Benjamin Cummings, London, UK, 1985, 115-142), Hilliger (BioTec 2, 40-44, 1991), Eggeling (Amino Acids 6: 261-272, 1994), Jetten und Sinskey (Critical Reviews in Biotechnology 15, 73-103, 1995) und Sahm et al. (Annuals of the New York Academy of Science 782, 25-39, 1996). To improve the performance characteristics of this Microorganisms become methods of mutagenesis, selection and mutant selection applied. For several years now also methods of recombinant DNA technology Strain improvement of amino acid producing strains of Corynebacterium by using single amino acid Biosynthesis genes amplified and the effect on Amino acid production examined. Review article on this can be found at Kinoshita ("Glutamic Acid Bacteria ", in: Biology of Industrial Microorganisms, Demain and Solomon (Eds.), Benjamin Cummings, London, UK, 1985, 115-142), Hilliger (BioTec 2, 40-44, 1991), Eggeling (Amino Acids 6: 261-272, 1994), Jetten and Sinskey (Critical Reviews in Biotechnology 15, 73-103, 1995) and Sahm et al. (Annuals of the New York Academy of Science 782, 25-39, 1996).

Um den eigenen Bedarf an verschiedenen Stoffen genau abzudecken, können Organismen ihre Stoffwechselwege sehr präzise regulieren. Hierfür besteht einerseits die Möglichkeit z. B. durch Regulation der Proteinsynthese die Menge der in der Zelle vorhandenen Enzyme eines Synthesewegs zu kontrollieren (sogenannte Langzeitkontrolle), andererseits sind verschiedenste Proteine, wie z. B. viele Enzyme eines Synthesewegs in ihrer Aktivität über deren Phosphorylierungszustand regulierbar (Kurzzeitkontrolle). Der Phosphorylierungszustand eines Proteins wirkt sich auf dessen Konfiguration aus und "schaltet" das Protein in seiner Aktivität "an" oder "ab". Auch regulatorische Proteine können durch Änderung des Phosphorylierungszustandes ihre Sekundär-, Tertiär- oder Quartärstruktur und damit ihre Affinität zu dem/den von ihnen kontrollierten Protein(en) ändern. Daher können die einzelnen Abläufe innerhalb von Zellen sehr genau aufeinander abgestimmt werden. (Proteomics - Posttranslational modifications, Vol. 1, No. 2 Pages 167-364 (2001)). To meet your own needs for various fabrics organisms can cover their metabolic pathways very much regulate precisely. On the one hand there is the Possibility z. B. by regulating protein synthesis Amount of enzymes present in the cell Control synthesis routes (so-called Long-term control), on the other hand, are very different Proteins such as B. many enzymes of a synthetic route in their Activity adjustable via their phosphorylation state (Short-term control). The phosphorylation state of a Proteins affects its configuration and "switches" the protein on or off in its activity. Regulatory proteins can also be modified by changing the Phosphorylation state their secondary, tertiary or Quaternary structure and thus its affinity for the change their controlled protein (s). Therefore, the individual processes within cells very precisely be coordinated. (Proteomics - Posttranslational modifications, Vol. 1, No. 2 Pages 167-364 (2001)).

Aufgabe der vorliegenden Erfindung war es, neue Hilfsmittel zur verbesserten fermentativen Herstellung von Feinchemikalien, insbesondere Aminosäuren, Nukleosiden, Nukleotiden und Vitaminen, bereitzustellen. The object of the present invention was to develop new aids for the improved fermentative production of Fine chemicals, especially amino acids, nucleosides, To provide nucleotides and vitamins.

Diese Aufgabe wird gelöst durch einen gentechnisch veränderten Mikroorganismus, in dem wenigstens ein Protein in seiner Phosphorylierbarkeit dauerhaft so geändert sind, daß eine gegenüber dem Wildtyp erhöhte Biosynthese wenigstens einer von dem Mikroorganismus synthetisierten Feinchemikalie erreicht wird. This task is solved by a genetic engineering modified microorganism in which at least one protein are permanently changed in their phosphorylability, that an increased biosynthesis compared to the wild type at least one synthesized by the microorganism Fine chemical is achieved.

Wenn im folgenden eine Aminosäure erwähnt wird, ist damit nicht nur die Base, sondern auch eines der üblichen Salze dieser Aminosäure, wie z. B. Lysin-Monohydrochlorid oder Lysin-Sulfat gemeint. Wenn im folgenden ein Vitamin genannt wird, ist damit jede verabreichbare Form des Vitamins oder von Vitaminprodukten gemeint. Die Bezeichnung der Nukleoside, Nukleotide, Antibiotika, Lipide und Pigmente wird in der üblichen Weise verwendet, jedoch fällt auch hier jede verabreichbare Form dieser Substanzen unter die vorliegende Erfindung. Desweiteren fällt bei Erwähnung von organischen Säuren sowohl die reine Säure, als auch jedes ihrer Salze und jede andere Darstellungsform unter die vorliegende Erfindung. If an amino acid is mentioned in the following, it is not just the base, but also one of the common salts this amino acid, e.g. B. lysine monohydrochloride or Lysine sulfate meant. If called a vitamin in the following is any administrable form of the vitamin or of vitamin products. The name of the Nucleosides, nucleotides, antibiotics, lipids and pigments is used in the usual way, but also falls here any administrable form of these substances under the present invention. Furthermore, when mentioning organic acids, both the pure acid and each their salts and any other form of presentation under the present invention.

Gemäß der Erfindung synthetisiert ein Mikroorganismus, in dem wenigstens ein Protein in seinem Phosphorylierungszustand gegenüber dem natürlich in dem verwendeten Organismus vorkommenden Protein so geändert ist, eine erhöhte Menge einer gewünschten Feinchemikalie. According to the invention, a microorganism synthesizes in who has at least one protein in his Phosphorylation state over that of course in the the protein used in the organism is changed is an increased amount of a desired fine chemical.

Unter "Feinchemikalien" sind in der vorliegenden Anmeldung alle Stoffwechselprodukte eines verwendeten Mikroorganismus zu verstehen, deren Herstellung in reiner Form oder als Gemisch gewünscht sind. Bevorzugte Feinchemikalien sind Aminosäuren, insbesondere L-Aminosäuren, bevorzugt L-Lysin, L-Threonin, L-Glutaminsäure, L-Methionin, L-Cystein, L- Cystin, L-Tyrosin, L-Phenylalanin, L-Tryptophan, L-Valin, L-Arginin, L-Leucin, L-Serin, L-Histidin, L-Asparaginsäure, L-Asparagin, L-Glutamin, L-Glycin, L-Prolin, L-Homoserin und L-Isoleucin oder Aminosäuregemische; Vitamine, insbesondere Pantothensäure, Riboflavin, Vitamin A, Vitamin E, Thiamin, Biotin, Folsäure, Ascorbinsäure, Calciferol, Phyllochinon, Menachinon, Vitamin B6, Vitamin B12, Nicotinsäure oder Vitamingemische; Nukleoside, insbesondere Adenosin, Guanosin, Thymidin, Uridin und Cytidin, aber auch modifizierte Nucleoside wie Inosin und Xanthin, sowie Nukleotide, insbesondere Adenylat, Guanylat, Thymidylat, Uridylat und Cytidylat, aber auch modifizierte Nukleotide wie Inosinat und Xanthylat; Chlorophylle, Bakteriochlorophylle und andere Tetrapyrrole; Pigmente wie Lutein, Lycopen, Astaxanthin, Cantaxanthin, Zeaxanthin, Neoxanthin, Violaxanthin, Phycocyanine und Phycoerythrine sowie andere Carotinoide mitsamt ihrer Derivate; Antibiotika wie Aminoglycoside, antifungale Antibiotika, Cephalosporine, betα-Lactam Antibiotika, Chloramphenicol und seine Derivate, Macrolide, Penicilline, Tetracycline und alle übrigen Antibiotika sowie ihre Derivate und Gemische; Enzyme für alle Anwendungen wie Phytasen, Amylasen und Glucoamylasen, Pektinasen, Lipasen, Penicillin-Acylasen, saure, neutrale und basische Proteasen, Cellulasen, Lactase, Isomerasen, Invertase, Pullulanase, Hydantoinasen, Glucanasen oder Anthocyanasen; Organische Säuren wie Citronensäure, Milchsäure, Gluconsäure, Buttersäure, Essigsäure, Ameisensäure, Bernsteinsäure, Ketoglutarsäure und deren Derivate; Polymere auf biologischer Basis wie Polyhydroxybuttersäure oder Polyalkanoate; Ergotalkaloide und andere Toxine; Lipide wie Mono-, Di- und Triglyceride, freie gesättigte und ungesättigte Fettsäuren, Phospho- und Glucolipide, sowie Sphingophospholipide, Cholin, Betain und Sarcosin; Steroide wie Chloesterin mitsamt seiner Derivate, Hopanoide, Insektenhormone, Pflanzensteroide wie Campesterin, Stigmasterin oder Sitosterin, Herzglycoside, Ecdyson, Brassinosteroide, Isoprenoide wie etwa alle Terpene, Steroidhormone wie etwa Testosteron, Östrogene, Progesterone, Cortison und andere Corticosteroide mitsamt ihrer Derivate, Aldosteron und alle Derivate, Gestagen und Progesteron, Androgene sofern noch nicht aufgeführt, Gallensäuren und Derivate; alle anderen Metabolite. Desweiteren kann die gezielte Änderung des Phosphorylierungszustandes von Proteinen genutzt werden, um möglichst viel Biomasse aufzubauen und so beispielsweise single cell Protein oder Starterkulturen bereit zu stellen. "Fine chemicals" in the present application all metabolic products of a microorganism used to understand, their production in pure form or as Mixture are desired. Preferred fine chemicals are Amino acids, especially L-amino acids, preferably L-lysine, L-threonine, L-glutamic acid, L-methionine, L-cysteine, L- Cystine, L-tyrosine, L-phenylalanine, L-tryptophan, L-valine, L-arginine, L-leucine, L-serine, L-histidine, L-aspartic acid, L-asparagine, L-glutamine, L-glycine, L-proline, L-homoserine and L-isoleucine or amino acid mixtures; vitamins, especially pantothenic acid, riboflavin, vitamin A, vitamin E, thiamine, biotin, folic acid, ascorbic acid, calciferol, Phylloquinone, menaquinone, vitamin B6, vitamin B12, Nicotinic acid or vitamin mixtures; Nucleosides, in particular Adenosine, guanosine, thymidine, uridine and cytidine, but also modified nucleosides such as inosine and xanthine, as well Nucleotides, especially adenylate, guanylate, thymidylate, Uridylate and cytidylate, but also modified nucleotides such as inosinate and xanthylate; chlorophylls, Bacteriochlorophylls and other tetrapyrroles; Pigments like Lutein, lycopene, astaxanthin, cantaxanthin, zeaxanthin, Neoxanthin, Violaxanthin, Phycocyanine and Phycoerythrine as well as other carotenoids together with their derivatives; Antibiotics such as aminoglycosides, antifungal antibiotics, Cephalosporins, betα-lactam antibiotics, chloramphenicol and its derivatives, macrolides, penicillins, tetracyclines and all other antibiotics and their derivatives and mixtures; Enzymes for all applications such as phytases, Amylases and glucoamylases, pectinases, lipases, Penicillin acylases, acidic, neutral and basic Proteases, cellulases, lactase, isomerases, invertase, Pullulanase, hydantoinases, glucanases or anthocyanases; Organic acids such as citric acid, lactic acid, Gluconic acid, butyric acid, acetic acid, formic acid, Succinic acid, ketoglutaric acid and their derivatives; Bio-based polymers such as polyhydroxybutyric acid or polyalkanoates; Ergot alkaloids and other toxins; Lipids such as mono-, di- and triglycerides, free saturated and unsaturated fatty acids, phospho- and glucolipids, as well as sphingophospholipids, choline, betaine and sarcosine; Steroids like chloesterin and its derivatives, Hopanoids, insect hormones, plant steroids such as Campesterin, stigmasterin or sitosterol, cardiac glycosides, Ecdyson, brassinosteroids, isoprenoids such as all Terpenes, steroid hormones such as testosterone, estrogens, Progesterone, cortisone and other corticosteroids together their derivatives, aldosterone and all derivatives, progestogens and Progesterone, androgens if not already listed, Bile acids and derivatives; all other metabolites. Furthermore, the targeted change of Phosphorylation state of proteins can be used to build up as much biomass as possible and so for example to provide single cell protein or starter cultures.

Die Mikroorganismen, die Gegenstand der vorliegenden Erfindung sind, können die Feinchemikalien aus verschiedenen Kohlenstoffquellen herstellen. Bevorzugte Ausgangssubstanzen sind hierbei Glucose, Saccharose, Lactose, Fructose, Maltose, Melasse, Stärke, Cellulose oder aus Acetat, Glycerin, Lactat oder Ethanol herstellen. Es kann sich um Vertreter von Prokaryonten, insbesondere Bakterien, z. B. coryneformer Bakterien, insbesondere der Gattung Corynebacterium, oder von E. coli, Archaebakterien insbesondere der Gattung Thermus, Hefen, oder Pilzen handeln. Bei der Gattung Corynebacterium ist insbesondere die Art Corynebacterium glutamicum zu nennen, die in der Fachwelt für ihre Fähigkeit bekannt ist, L-Aminosäuren zu produzieren. The microorganisms that are the subject of the present Invention are, the fine chemicals can different carbon sources. preferred The starting substances here are glucose, sucrose, Lactose, fructose, maltose, molasses, starch, cellulose or make from acetate, glycerin, lactate or ethanol. It can be representatives of prokaryotes, in particular Bacteria, e.g. B. coryneform bacteria, especially the Genus Corynebacterium, or from E. coli, archaebacteria especially the genus Thermus, yeast, or mushrooms act. In the genus Corynebacterium is particularly to name the species Corynebacterium glutamicum, which in the Experts are known for their ability to make L-amino acids to produce.

Geeignete Stämme der Gattung Corynebacterium, insbesondere der Art Corynebacterium glutamicum, sind die zum Beispiel bekannten Wildtypstämme
Corynebacterium glutamicum ATCC13032
Corynebacterium acetoglutamicum ATCC15806
Corynebacterium acetoacidophilum ATCC13870
Corynebacterium thermoaminogenes FERM BP-1539
Corynebacterium melassecola ATCC17965
Brevibacterium flavum ATCC14067
Brevibacterium lactofermentum ATCC13869 und
Brevibacterium divaricatum ATCC14020
und daraus hergestellte L-Lysin produzierende Mutanten bzw. Stämme, wie beispielsweise
Corynebacterium glutamicum FERM-P 1709
Brevibacterium flavum FERM-P 1708
Brevibacterium lactofermentum FERM-P 1712
Corynebacterium glutamicum FERM-P 6463
Corynebacterium glutamicum FERM-P 6464 und
Corynebacterium glutamicum DSM5715. Suitable strains of the genus Corynebacterium, in particular of the species Corynebacterium glutamicum, are the wild-type strains known for example
Corynebacterium glutamicum ATCC13032
Corynebacterium acetoglutamicum ATCC15806
Corynebacterium acetoacidophilum ATCC13870
Corynebacterium thermoaminogenes FERM BP-1539
Corynebacterium melassecola ATCC17965
Brevibacterium flavum ATCC14067
Brevibacterium lactofermentum ATCC13869 and
Brevibacterium divaricatum ATCC14020
and L-lysine-producing mutants or strains produced therefrom, for example
Corynebacterium glutamicum FERM-P 1709
Brevibacterium flavum FERM-P 1708
Brevibacterium lactofermentum FERM-P 1712
Corynebacterium glutamicum FERM-P 6463
Corynebacterium glutamicum FERM-P 6464 and
Corynebacterium glutamicum DSM5715.

Außerdem sind Enterobacteriaceae, insbesondere Escherichia coli für die Synthese von Feinchemikalien besonders geeignet. Geeignete L-Threonin produzierende Stämme der Gattung Escherichia, insbesondere der Art Escherichia coli sind beispielsweise
Escherichia coli TF427
Escherichia coli H4578
Escherichia coli KY10935
Escherichia coli EL1003
Escherichia coli VNIIgenetika MG-442
Escherichia coli VNIIgenetika VL334/pYN7
Escherichia coli VNIIgenetika M1
Escherichia coli VNIIgenetika 472T23
Escherichia coli VNIIgenetika TDH-6
Escherichia coli BKIIM B-3996
Escherichia coli BKIIM B-5318
Escherichia coli B-3996-C43
Escherichia coli B-3996-C80
Escherichia coli B-3996/pTWV-pps
Escherichia coli B-3996(pMW::THY)
Escherichia coli B-3996/pBP5
Escherichia coli kat 13
Escherichia coli KCCM-10132. Enterobacteriaceae, in particular Escherichia coli, are also particularly suitable for the synthesis of fine chemicals. Suitable strains of the genus Escherichia, in particular of the species Escherichia coli, which produce L-threonine are, for example
Escherichia coli TF427
Escherichia coli H4578
Escherichia coli KY10935
Escherichia coli EL1003
Escherichia coli VNII genetics MG-442
Escherichia coli VNIIgenetics VL334 / pYN7
Escherichia coli VNIIgenetics M1
Escherichia coli VNIIgenetics 472T23
Escherichia coli VNIIgenetics TDH-6
Escherichia coli BKIIM B-3996
Escherichia coli BKIIM B-5318
Escherichia coli B-3996-C43
Escherichia coli B-3996-C80
Escherichia coli B-3996 / pTWV-pps
Escherichia coli B-3996 (pMW :: THY)
Escherichia coli B-3996 / pBP5
Escherichia coli kat 13
Escherichia coli KCCM-10132.

Geeignete L-Threonin produzierende Stämme der Gattung Serratia, insbesondere der Art Serratia marcecsens sind beispielsweise
Serratia marcescens HNr21
Serratia marcescens TLr156
Serratia marcescens T2000. Suitable strains of the genus Serratia, in particular of the species Serratia marcecsens, which produce L-threonine are, for example
Serratia marcescens HNr21
Serratia marcescens TLr156
Serratia marcescens T2000.

Geeignete D-Pantothensäure produzierende Stämme der Gattung Escherichia, insbesondere der Art Escherichia coli sind beispielsweise
Escherichia coli FV5069 pFV31
Escherichia coli FV5069 pFV202
Escherichia coli FE6b pFE80
Escherichia coli KE3. Suitable strains of the genus Escherichia, in particular of the type Escherichia coli, which produce D-pantothenic acid are, for example
Escherichia coli FV5069 pFV31
Escherichia coli FV5069 pFV202
Escherichia coli FE6b pFE80
Escherichia coli KE3.

Geeignete D-Pantothensäure produzierende Stämme der Gattung Bacillus, insbesondere der Art Bacillus subtilis sind unter anderen beispielsweise die in der WO 01/21772 genannten Stämme
Bacillus subtilis Stamm PA 221
Bacillus subtilis Stamm PA 248
Bacillus subtilis Stamm PA 236
Bacillus subtilis Stamm PA 221/pAN429-4
Bacillus subtilis Stamm PA 413-4
Bacillus subtilis Stamm PA 236-1
Bacillus subtilis Stamm PA 340
Bacillus subtilis Stamm PA 377
Bacillus subtilis Stamm PA 365
Bacillus subtilis Stamm PA 377-2
Bacillus subtilis Stamm PA 824-2. Suitable strains of the genus Bacillus producing D-pantothenic acid, in particular of the species Bacillus subtilis, include the strains mentioned in WO 01/21772
Bacillus subtilis strain PA 221
Bacillus subtilis strain PA 248
Bacillus subtilis strain PA 236
Bacillus subtilis strain PA 221 / pAN429-4
Bacillus subtilis strain PA 413-4
Bacillus subtilis strain PA 236-1
Bacillus subtilis strain PA 340
Bacillus subtilis strain PA 377
Bacillus subtilis strain PA 365
Bacillus subtilis strain PA 377-2
Bacillus subtilis strain PA 824-2.

Geeignete Riboflavin produzierende Stämme der Gattung Bacillus, insbesondere der Art Bacillus subtilis sind beispielsweise
Bacillus subtilis RB50
Bacillus subtilis RB58
Bacillus subtilis B18502. Suitable strains of the genus Bacillus producing riboflavin, in particular of the species Bacillus subtilis, are for example
Bacillus subtilis RB50
Bacillus subtilis RB58
Bacillus subtilis B18502.

Geeignete Riboflavin produzierende Mikroorganismen sind Ashbya, insbesondere die Art Ashbya gossypii, Eremothecium ashbyii, Candida flareri oder Bacillus subtilis. Suitable riboflavin producing microorganisms are Ashbya, especially the species Ashbya gossypii, Eremothecium ashbyii, Candida flareri or Bacillus subtilis.

Gemäß der Erfindung sind außerdem andere procaryote Organismen einsetzbar, z. B. Streptomyceten, Actinomyceten und andere Gram-positive Bakterien wie Bacillus subtilis oder Clostridium acetobutylicum, oder auch Lactobacillen, wie z. B. Lactococcus lactis oder Lactobacillus acidophilus. Ebenso sind eucaryotisch Zellen zur Herstellung gewünschter Feinchemikalien einsetzbar. Hierfür können alle kultivierbaren Eukaryontenzellen verwendet werden, die ein gemäß der Erfindung gewünschtes Stoffwechselprodukt herstellen können. Insbesondere geeignet sind Hefen wie z. B. Saccharomyces cerevisiae, Schizosaccharomyces pombe, Hansenula polymorpha, Candida bondii, Kluyveromyces lactis, Schwanniomyces occidentalis oder Pichia pastoris, filamentöse Pilze wie Aspergillus niger, Aspergillus nidulans, Ashbya gossipii oder Neurospora crassa, Insektenzellsystem wie z. B. das Baculovirus-System und gängige Zelllinien von Säugetierzellen, wie z. B. HELA- Zellen, COS-Zellen oder CHO-Zellen sowie Algen. Other procaryotes are also according to the invention Organisms can be used, e.g. B. Streptomycetes, Actinomycetes and other Gram positive bacteria such as Bacillus subtilis or Clostridium acetobutylicum, or also lactobacilli, such as B. Lactococcus lactis or Lactobacillus acidophilus. Eucaryotic cells are also desired for the production Fine chemicals can be used. Everyone can do this cultivatable eukaryotic cells can be used that a Desired metabolite according to the invention can manufacture. Yeasts such as z. B. Saccharomyces cerevisiae, Schizosaccharomyces pombe, Hansenula polymorpha, Candida bondii, Kluyveromyces lactis, Schwanniomyces occidentalis or Pichia pastoris, filamentous mushrooms such as Aspergillus niger, Aspergillus nidulans, Ashbya gossipii or Neurospora crassa, Insect cell system such as B. the baculovirus system and common cell lines of mammalian cells, such as B. HELA- Cells, COS cells or CHO cells as well as algae.

Die Erhöhung der produzierten Menge einer gewünschten Feinchemikalie wird gemäß der vorliegenden Erfindung dadurch erreicht, daß wenigstens ein Protein des Mikroorganismus in seinem Phosphorylierungszustand geändert wird, verglichen zu dem natürlich vorkommenden Protein des Mikroorganismus. Daher zeichnet sich ein Mikroorganismus der vorliegenden Erfindung dadurch aus, daß er wenigstens ein Protein enthält, daß sich in seiner Aminosäuresequenz in wenigstens einer Aminosäure gegenüber dem selben natürlich in diesem Organismus vorkommenden Protein unterscheidet, wobei eine Aminosäure betroffen ist, die in dem Protein als Phosphorylierungsstelle dienen kann. Der Unterschied gegenüber der Wildtypsequenz kann in einer Mutation, insbesondere einem Aminosäureaustausch oder einer Deletion oder Insertion wenigstens einer Aminosäure bestehen. Durch den Austausch, die Deletion oder Insertion einer Aminosäure kann eine im natürlich vorkommenden Protein vorhandene Phosphorylierungsstelle entfernt werden, eine zusätzliche Phosphorylierungsstelle gegenüber dem natürlich vorkommenden Protein eingeführt werden, oder eine Phosphorylierungsstelle in ihrem Phosphorylierungszustand dauerhaft geändert werden (z. B. eine vorhandene Phosphorylierungsstelle liegt dauerhaft phosphoryliert, oder dauerhaft unphosphoryliert vor). Increasing the amount produced of a desired one Fine chemical is made according to the present invention achieved in that at least one protein of Microorganism changed in its phosphorylation state is compared to the naturally occurring protein of Microorganism. Therefore, a microorganism is distinguished the present invention in that at least a protein contains that in its amino acid sequence in at least one amino acid versus the same protein naturally occurring in this organism distinguishes, whereby an amino acid is affected, which in the protein can serve as a phosphorylation site. The Difference from the wild type sequence can be in one Mutation, in particular an amino acid exchange or one Deletion or insertion of at least one amino acid consist. By exchange, deletion or insertion An amino acid can be one that occurs naturally Protein present phosphorylation site are removed, an additional phosphorylation site over the naturally occurring protein are introduced, or one Phosphorylation site in its phosphorylation state permanently changed (e.g. an existing Phosphorylation site is permanently phosphorylated, or permanently unphosphorylated before).

Um die Änderung des Phosphorylierbarkeit zu erreichen, insbesondere die dauerhaften Änderung des Phosphorylierungszustandes eines Proteins, kann eine oder mehrere im natürlichen Protein vorkommende Aminosäure(n), die als Phosphorylierungsstelle in dem Protein dient/dienen, gegen eine andere Aminosäure ausgetauscht werden, so daß der zur Regulation des Proteins dienende Phosphorylierungszustand dauerhaft geändert wird. Es können auch durch Austausch einer oder mehrerer Aminosäuren weitere zusätzliche Phosphorylierungsstellen in ein Enzym eingeführt werden. To achieve the change in phosphorylability, in particular the permanent change of the Phosphorylation state of a protein, one or several amino acids occurring in the natural protein, that as a phosphorylation site in the protein serves / serve, exchanged for another amino acid be so that the serving for the regulation of the protein Phosphorylation status is changed permanently. It can also by exchanging one or more amino acids additional phosphorylation sites in an enzyme be introduced.

Die Änderung des Phosphorylierungszustandes kann auf jede Weise erfolgen, die zu einer solchen Änderung führt, bevorzugt wird der Phosphorylierungszustand durch Mutagenese der proteincodierenden DNA-Sequenz geändert, besonders bevorzugt durch gerichtete Mutagenese ("sitedirected mutagenesis") der proteincodierenden DNA-Sequenz. The change in the state of phosphorylation can affect any Way that leads to such a change, the phosphorylation state is preferred by Mutagenesis of the protein coding DNA sequence changed, particularly preferred by directed mutagenesis ("site-directed mutagenesis") of the protein-coding DNA sequence.

Geeignete Aminosäureaustausche sind: Suitable amino acid exchanges are:

1. Austausche, die eine Phosphorylierungsstelle entfernen1. Replacements that remove a phosphorylation site

Austausch von Serin, Threonin, Tyrosin, Aspartat, Glutamat Histidin, Arginin oder Lysin gegen eine andere Aminosäure, wobei ein Austausch gegen jede andere Aminosäure gemeint ist (also auch gegen eine andere der hier genannten). Ein besonders bevorzugter Austausch ist der Austausch von Serin oder Threonin gegen Asparagin, Glutamin, Glycin, Alanin, Cystein, Valin, Methionin, Isoleucin, Leucin, Phenylalanin, Histidin, Lysin, Tryptophan oder Arginin. Exchange of serine, threonine, tyrosine, aspartate, glutamate Histidine, arginine or lysine against another amino acid, meaning an exchange for any other amino acid is (also against another of the above). On a particularly preferred exchange is the exchange of serine or threonine against asparagine, glutamine, glycine, alanine, Cysteine, valine, methionine, isoleucine, leucine, phenylalanine, Histidine, lysine, tryptophan or arginine.

2. Austausche, die eine Phosphorylierungsstelle einführen2. Exchanges that introduce a phosphorylation site

Austausch einer Aminosäure, die nicht Serin, Threonin, Tyrosin, Asparaginsäure, Glutaminsäure, Histidin, Arginin oder Lysin ist, gegen eine dieser Aminosäuren. Replacement of an amino acid other than serine, threonine, Tyrosine, aspartic acid, glutamic acid, histidine, arginine or lysine, against one of these amino acids.

3. Austausche, bei denen eine vorhandene potentielle Phophorylierungsstelle nicht mehr regulierbar ist, nämlich, indem dauerhafte Phosphorylierung oder dauerhafte Dephosphorylierung auftritt3. Exchange where there is an existing potential Phosphorylation site is no longer regulated, namely, by permanent phosphorylation or permanent dephosphorylation occurs

Austausch von Serin, Threonin, Histidin oder Tyrosin gegen Glutaminsäure, Asparaginsäure, Alanin, Glycin oder Valin. Exchange of serine, threonine, histidine or tyrosine for Glutamic acid, aspartic acid, alanine, glycine or valine.

4. Austausche im Bereich der "Phosphorylierungssequenz"4. Exchange in the area of the "phosphorylation sequence"

Als "Phosphorylierungssequenz" wird hierin die Aminosäuresequenz des Proteins bezeichnet, die die im Wildtyp reversibel phosphorylierte Aminosäure umgibt. Diese Aminosäuresequenzen sind dem Fachmann bekannt und dienen der "Erkennung" der zu phosphorylierenden Aminosäure. Jeder Austausch einer der Aminosäuren in dieser Sequenz, der dazu führt, daß eine sich in dieser Sequenz befindliche Aminosäure nicht mehr reversibel phosphorylierbar/dephosphorylierbar ist, ist als unter die vorliegende Erfindung fallend zu betrachten. The "phosphorylation sequence" is used herein Amino acid sequence of the protein referred to that in the Surrounds wild-type reversibly phosphorylated amino acid. This Amino acid sequences are known to the person skilled in the art and serve the "recognition" of the amino acid to be phosphorylated. Everyone Exchange one of the amino acids in this sequence, the addition leads to one in this sequence Amino acid no longer reversible phosphorylierbar / dephosphorylierbar is considered to be among the to consider the present invention as falling.

Die in einem natürlichen Protein vorkommenden Aminosäuren sind dem Fachmann bekannt. Gemäß der Erfindung können alle natürlich vorkommenden Aminosäuren gegeneinander ausgetauscht werden, einschließlich der posttranslational modifizierten Aminosäuren. The amino acids found in a natural protein are known to the person skilled in the art. According to the invention, everyone can naturally occurring amino acids against each other exchanged, including post-translational modified amino acids.

Die auszutauschende(n) Aminosäure(n) befindet/befinden sich erfindungsgemäß in einer Aminosäuresequenz innerhalb des Proteins, die die betrachtete Aminosäure einer reversiblen Phosphorylierung zugänglich macht. Diese Aminosäuresequenzen sind dem Fachmann bekannt. Die auszutauschende(n) Aminosäure(n) kann/können sich im Bereich eines aktiven Zentrums eines Proteins, insbesondere eines Enzyms befinden, oder außerhalb des aktiven Zentrums in einem Bereich, über dessen Phosphorylierungszustand die Aktivität des Proteins oder dessen Affinität zu anderen Proteinen kontrolliert wird. The amino acid (s) to be exchanged is / are according to the invention in an amino acid sequence within the Protein, which is the considered amino acid of a reversible Makes phosphorylation accessible. This Amino acid sequences are known to the person skilled in the art. The amino acid (s) to be exchanged can be found in the Area of an active center of a protein, in particular of an enzyme, or outside the active center in an area over whose phosphorylation state the Activity of the protein or its affinity for others Proteins is controlled.

Die Proteine, die gemäß der vorliegenden Erfindung in ihrem Phosphorylierungszustand geändert werden, können Enzyme, regulatorische Proteine, strukturbildende Proteine, Transport-Proteine, Speicher-Proteine. Proteine mit Immunfunktion oder Komponenten von Signaltransduktionswegen sein. The proteins according to the present invention in their Phosphorylation state can be changed, enzymes, regulatory proteins, structure-forming proteins, Transport proteins, storage proteins. Proteins with Immune function or components of signal transduction pathways his.

Als Zielprotein für eine erfindungsgemäße Änderung des Phosphorylierungszustandes kann jedes Protein verwendet werden, bei dem eine Änderung des Phosphorylierungszustandes eine Erhöhung der Syntheserate einer gewünschten Feinchemikalie bewirkt, oder auch die Syntheserate nicht gewünschter Stoffwechselprodukte erniedrigt. Beispiele für Proteine, die als unter den Umfang der Erfindung fallend anzusehen sind, sind:

  • - Proteine des Biosynthesewegs der entsprechenden Feinchemikalie, deren Synthese gewünscht ist, insbesondere Schlüsselenzyme des Synthesewegs.
  • - Proteine, die Biosynthesewege von gewünschten Feinchemikalien regulieren (feed-forward oder feed-back).
  • - Proteine der Signalerkennung und -weiterleitung.
  • - Proteine der Genregulation.
  • - Proteine, die in die Zellintegrität involviert sind und dadurch den Biokatalysator erhalten und/oder modifizieren.
  • - Komponenten des Proteinsekretionsapparates.
  • - Proteine mit Immunfunktionen.
  • - Proteine mit Speicherfunktion.
  • - Proteine mit Transportfunktion.
Any protein in which a change in the phosphorylation state brings about an increase in the synthesis rate of a desired fine chemical or else the synthesis rate of undesired metabolic products can be used as the target protein for a change in the phosphorylation state according to the invention. Examples of proteins that are considered to be within the scope of the invention are:
  • - Proteins of the biosynthetic pathway of the corresponding fine chemical, the synthesis of which is desired, in particular key enzymes of the synthetic pathway.
  • - Proteins that regulate the biosynthetic pathways of desired fine chemicals (feed-forward or feed-back).
  • - Proteins of signal recognition and transmission.
  • - Proteins of gene regulation.
  • - Proteins that are involved in cell integrity and thereby maintain and / or modify the biocatalyst.
  • - Components of the protein secretion apparatus.
  • - Proteins with immune functions.
  • - Proteins with a storage function.
  • - Proteins with a transport function.

Insbesondere können als Zielprotein ein oder mehrere Enzyme aus der folgenden Liste von Proteinen in den angegebenen Spezies ausgewählt werden. Enzyme aus E.coli mit EC-Nummer 1.1.1.1 Alkoholdehydrogenase
1.1.1.100 3-Oxoacyl-[Acyl-Carrierprotein]-reductase
1.1.1.103 L-Threonin-3-dehydrogenase
1.1.1.105 Retinoldehydrogenase
1.1.1.122 D-threo-Aldose-1-dehydrogenase
1.1.1.125 2-Desoxy-D-gluconat-3-dehydrogenase
1.1.1.132 GDP-Mannose-6-dehydrogenase
1.1.1.133 DTDP-4-Dehydrorhamnosereductase
1.1.1.14 L-Iditol-2-dehydrogenase
1.1.1.140 Sorbitol-6-phosphat-2-dehydrogenase
1.1.1.145 3β-Hydroxy-Δ5-Steroiddehydrogenase
1.1.1.146 11β-Hydroxysteroiddehydrogenase
1.1.1.157 3-Hydroxybutyryl-CoA-Dehydrogenase
1.1.1.158 UDP-N-Acetylmuramatdehydrogenase
1.1.1.159 7α-Hydroxysteroiddehydrogenase
1.1.1.17 Mannitol-1-phosphat-5-dehydrogenase
1.1.1.179 D-Xylose-1-dehydrogenase (NADP+)
1.1.1.18 Myo-Inositol-2-dehydrogenase
1.1.1.188 Prostaglandin F-Synthase
1.1.1.195 Cinnamyl-Alkoholdehydrogenase
1.1.1.2 Alkoholdehydrogenase(NADP+)
1.1.1.202 1,3-Propandioldehydrogenase
1.1.1.204 Xanthindehydrogenase
1.1.1.205 IMP-Dehydrogenase
1.1.1.21 Aldehydreductase
1.1.1.211 Lange Ketten-3-hydroxyacyl-CoA-Dehydrogenase
1.1.1.218 Morphin-6-dehydrogenase
1.1.1.22 UDP-Glucose-6-dehydrogenase
1.1.1.23 Histidinoldehydrogenase
1.1.1.233 N-Acylmannosamin-1-dehydrogenase
1.1.1.236 Tropinonreductase
1.1.1.24 Chinat-5-dehydrogenase
1.1.1.244 Methanoldehydrogenase
1.1.1.25 Shikimat-5-dehydrogenase
1.1.1.27 L-Lactatdehydrogenase
1.1.1.28 D-Lactatdehydrogenase
1.1.1.29 Glyceratdehydrogenase
1.1.1.3 Homoserindehydrogenase
1.1.1.30 3-Hydroxybutyratdehydrogenase
1.1.1.31 3-Hydroxyisobutyratdehydrogenase
1.1.1.35 3-Hydroxyacyl-CoA-Dehydrogenase
1.1.1.36 Acetoacetyl-CoA-Reductase
1.1.1.37 Malatdehydrogenase
1.1.1.38 Malatdehydrogenase (Oxalacetat-decarboxylierend)
1.1.1.40 Malatdehydrogenase (OxalAcetatdecarboxylierend)(NADP+)
1.1.1.42 Isocitratdehydrogenase (NADP+)
1.1.1.44 Phosphogluconatdehydrogenase (decarboxylierend)
1.1.1.47 Glucose-1-dehydrogenase
1.1.1.48 Galactose-1-dehydrogenase
1.1.1.49 Glucose-6-phosphat-1-dehydrogenase
1.1.1.56 Ribitol-2-dehydrogenase
1.1.1.57 Fructuronatreductase
1.1.1.58 Tagaturonatreductase
1.1.1.6 Glyceroldehydrogenase
1.1.1.61 4-Hydroxybutyratdehydrogenase
1.1.1.62 Östradiol-17β-dehydrogenase
1.1.1.69 Gluconat-5-dehydrogenase
1.1.1.77 Lactaldehydreductase
1.1.1.8 Glycerol-3-phosphatdehydrogenase(NAD+)
1.1.1.81 Hydroxypyruvatreductase
1.1.1.83 D-Malatdehydrogenase (decarboxylierend)
1.1.1.85 3-Isopropylmalatdehydrogenase
1.1.1.86 Ketol-Säure-Reductoisomerase
1.1.1.91 Aryl-Alkoholdehydrogenase (NADP+)
1.1.1.93 Tartratdehydrogenase
1.1.1.95 Phosphoglyceratdehydrogenase
1.1.2.3 L-Lactatdehydrogenase (Cytochrome)
1.1.2.4 D-Lactatdehydrogenase (Cytochrome)
1.1.3.13 Alkoholoxidase
1.1.3.15 (S)-2-Hydroxysäureoxidase
1.1.3.22 Xanthinoxidase
1.1.3.4 Glucoseoxidase
1.1.99.1 Cholindehydrogenase
1.1.99.10 Glucosedehydrogenase (Akzeptor)
1.1.99.17 Glucosedehydrogenase (Pyrrolochinolin-Chinon)
1.1.99.23 Polyvinyl-Alkoholdehydrogenase (Akzeptor)
1.1.99.5 Glycerol-3-phosphatdehydrogenase
1.1.99.6 D-2-Hydroxysäure-Dehydrogenase
1.1.99.8 Alkoholdehydrogenase (Akzeptor)
1.10.2.2 Ubichinol-Cytochrom c-Reductase
1.10.3.2 Laccase
1.10.3.3 L-Ascorbatoxidase
1.11.1.12 Phospholipid-Hydroperoxid-Glutathionperoxidase
1.11.1.5 Cytochrom c-Peroxidase
1.11.1.6 Katalase
1.11.1.7 Peroxidase
1.11.1.9 Glutathionperoxidase
1.12.1.2 Wasserstoffdehydrogenase
1.12.2.1 Cytochrom c3-Hydrogenase
1.13.11.2 Katechol-2,3-dioxygenase
1.13.11.8 Protocatechuat-4,5-dioxygenase
1.13.12.4 Lactat-2-monooxygenase
1.14.12.10 Benzoat-1,2-dioxygenase
1.14.12.11 Toluendioxygenase
1.14.12.12 Naphthalen-1,2-dioxygenase
1.14.12.3 Benzen-1,2-dioxygenase
1.14.13.2 4-Hydroxybenzoat-3-monooxygenase
1.14.13.20 2,4-Dichlorphenol-6-monooxygenase
1.14.13.25 Methanmonooxygenase
1.14.13.3 4-Hydroxyphenylacetat-3-monooxygenase
1.14.13.7 Phenol-2-monooxygenase
1.14.14.3 Alkanalmonooxygenase (FMH-verknüpft)
1.15.1.1 Superoxiddismutase
1.16.1.1 Quecksilber(II)reductase
1.17.4.1 Ribonucleosiddiphosphat-Reductase
1.17.4.2 Ribonucleosidtriphosphat-Reductase
1.18.1.1 Rubredoxin-NAD+-Reductase
1.18.1.2 Ferredoxin-NADP+-Reductase
1.18.1.3 Ferredoxin-NAD+-Reductase
1.18.1.4 Rubredoxin-NAD(P)+-Reductase
1.18.99.1 Hydrogenase
1.2.1.1 Formaldehyddehydrogenase (Glutathion)
1.2.1.10 Acetaldehyddehydrogenase (acetylierend)
1.2.1.11 Aspartatsemialdehyddehydrogenase
1.2.1.12 Glyceraldehyd-3-phosphatdehydrogenase (phosphorylierend)
1.2.1.16 Succinatsemialdehyddehydrogenase (NAD(P)+)
1.2.1.19 Aminobutyraldehyddehydrogenase
1.2.1.2 Formatdehydrogenase
1.2.1.22 Lactaldehyddehydrogenase
1.2.1.3 Aldehyddehydrogenase (NAD+)
1.2.1.38 N-Acetyl-γ-glutamylphosphatreductase
1.2.1.39 Phenylacetaldehyddehydrogenase
1.2.1.40 3α,7α,12α-Trihydroxycholestan-26-al
1.2.1.41 Glutamat-5-Semialdehyddehydrogenase
1.2.1.46 Formaldehyddehydrogenase
1.2.1.8 Betainaldehyddehydrogenase
1.2.1.9 Glyceraldehyd-3-phosphatdehydrogenase (NADP+)
1.2.2.2 Pyruvatdehydrogenase (Cytochrome)
1.2.3.1 Aldehydroxidase
1.2.4.1 Pyruvatdehydrogenase (Lipoamid)
1.2.4.2 Oxoglutaratdehydrogenase (Lipoamid)
1.2.7.1 Pyruvatsynthase
1.2.99.2 Kohlenmonoxiddehydrogenase
1.2.99.4 Formaldehyddismutase
1.2.99.5 Formylmethanfurandehydrogenase
1.3.1.12 Prephenatdehydrogenase
1.3.1.19 cis-1,2-Dihydrobenzen-1,2-diol-dehydrogenase
1.3.1.2 Dihydropyrimidindehydrogenase (NADP+)
1.3.1.24 Biliverdinreductase
1.3.1.26 Dihydrodipicolinatreductase
1.3.1.28 2,3-Dihydro-2,3-dihydroxybenzoatdehydrogenase
1.3.1.29 cis-1,2-Dihydro-1,2- dihydroxynaphthalendehydrogenase
1.3.1.8 Acyl-CoA-Dehydrogenase (NADP+)
1.3.1.9 Enoyl-[Acyl-Carrierprotein]-reductase (NADH)
1.3.3.1 Dihydroorotatoxidase
1.3.3.3 Coproporphyrinogenoxidase
1.3.3.4 Protoporphyrinogenoxidase
1.3.3.5 Bilirubinoxidase
1.3.5.1 Succinatdehydrogenase (Ubichinon)
1.3.99.1 Succinatdehydrogenase
1.3.99.10 Isovaleryl-CoA-Dehydrogenase
1.3.99.13 Lange Ketten-Acyl-CoA-Dehydrogenase
1.3.99.16
1.3.99.3 Acyl-CoA-Dehydrogenase
1.4.1.13 Glutamatsynthase (NADPH)
1.4.1.14 Glutamatsynthase (NADH)
1.4.1.4 Glutamatdehydrogenase (NADP+)
1.4.3.16 L-Aspartatoxidase
1.4.3.4 Aminoxidase (Flavin-enthaltend)
1.4.3.5 Pyridoxaminphosphatoxidase
1.4.3.6 Aminoxidase (Kupfer-enthaltend)
1.4.4.2 Glycindehydrogenase (decarboxylierend)
1.4.7.1 Glutamatsynthase (Ferredoxin)
1.4.99.1 D-Aminosäuredehydrogenase
1.5.1.12 1-Pyrrolin-5-carboxylatdehydrogenase
1.5.1.2 Pyrrolin-5-carboxylatreductase
1.5.1.20 Methylentetrahydrofolatreductase (NADPH)
1.5.1.3 Dihydrofolatreductase
1.5.1.5 Methylentetrahydrofolatdehydrogenase (NADP+)
1.5.3.1 Sarcosinoxidase
1.5.99.10
1.5.99.2 Dimethylglycindehydrogenase
1.5.99.4 Nicotindehydrogenase
1.5.99.7 Trimethylamindehydrogenase
1.5.99.8 Prolindehydrogenase
1.6.1.1 NAD(P)+-Transhydrogenase (b-specifisch)
1.6.2.4 NADPH-Ferrihämoproteinreductase
1.6.4.2 Glutathionreductase (NADPH)
1.6.4.5 Thioredoxinreductase (NADPH)
1.6.5.3 NADH-Dehydrogenase (Ubichinon)
1.6.5.4 Monodehydroascorbatreductase (NADH)
1.6.5.5 NADPH : Chinon-Reductase
1.6.6.1 Nitratreductase (NADH)
1.6.6.3 Nitratreductase (NADPH)
1.6.6.4 Nitritreductase (NAD(P)H)
1.6.6.8 GMP-Reductase
1.6.6.9 Trimethylamin-N-oxidreductase
1.6.8.1 NPLD(P)H-Dehydrogenase (FMN)
1.6.99.1 NADPH-Dehydrogenase
1.6.99.2 NAD(P)H-Dehydrogenase (Chinon)
1.6.99.3 NADH-Dehydrogenase
1.7.99.4 Nitratreductase
1.7.99.5 5,10-Methylentetrahydrofolatreductase (FADH2)
1.8.1.2 Sulfitreductase (NADPH)
1.8.1.4 Dihydrolipoamiddehydrogenase
1.8.4.5 Methionin-S-oxidreductase
1.8.4.6 Protein-Methionin-S-oxidreductase
1.8.7.1 Sulfitreductase (Ferredoxin)
1.8.99.4
1.9.3.1 Cytochrom c-Oxidase
1.97.1.4 [Pyruvat-Format-Lyase] aktivierendes Enzym
1.97.4.1
2.1.1.107 Uroporphyrin-III c-Methyltransferase
2.1.1.113 Sequenz-spezifische DNA-Methyltransferase (Cytosin-N4-spezifisch)
2.1.1.13 5-Methyltetrahydrofolat-Homocystein-S- Methyltransferase
2.1.1.14 5-Methyltetrahydropteroyltriglutamat-Homocystein
2.1.1.17 Phosphatidylethanolamin-N-Methyltransferase
2.1.1.31 tRNA (Guanin-N1-)-Methyltransferase
2.1.1.35 tRNA (Uracil-5-)-Methyltransferase
2.1.1.37 DNA (Cytosin-5-)-Methyltransferase
2.1.1.45 Thymidylatsynthase
2.1.1.48 rmA (Adenin-N6-)-Methyltransferase
2.1.1.63 methylierte DNA-Protein-Cystein-S- Methyltransferase
2.1.1.64 3-Demethylubichinon-9-3-O-Methyltransferase
2.1.1.72 Sequenz-spezifische DNA-Methyltransferase (Adeninspezifisch)
2.1.1.73 Sequenz-spezifische DNA-Methyltransferase (Cytosin-spezifisch)
2.1.1.77 Protein-L-Isoaspartat(D-Aspartat)-O- Methyltransferase
2.1.1.79 Cyclopropan-Fettsäure-Acyl-phospholipidsynthase
2.1.1.80 Protein-Glutamat-O-Methyltransferase
2.1.2.1 Glycin-Hydroxymethyltransferase
2.1.2.10 Aminomethyltransferase
2.1.2.11 3-Methyl-2-oxobutanoathydroxymethyltransferase
2.1.2.2 Phosphoribosylglycinamid-Formyltransferase
2.1.2.3 Phosphoribosylaminoimidazolcarboxamid- Formyltransferase
2.1.2.9 Methionyl-tRNA-Formyltransferase
2.1.3.2 Aspartat-Carbamoyltransferase
2.1.3.3 Ornithin-Carbamoyltransferase
2.2.1.1 Transketolase
2.2.1.2 Transaldolase
2.3.1.1 Aminosäure-N-Acetyltransferase
2.3.1.117 2,3,4, 5-Tetrahydropyridin-2-carboxylat-N- Succinyltransferase
2.3.1.118 N-Hydroxyarylamin-O-Acetyltransferase
2.3.1.12 Dihydrolipoamid-S-Acetyltransferase
2.3.1.128 Ribosomenprotein-Alanin-N-Acetyltransferase
2.3.1.129 Acyl-[Acyl-Carrierprotein]-UDP-N-
2.3.1.15 Glycerol-3-phosphat-O-Acyltransferase
2.3.1.16 Acetyl-CoA-C-Acyltransferase
2.3.1.18 Galactosid-O-Acetyltransferase
2.3.1.28 ChlorAMPhenicol-O-Acetyltransferase
2.3.1.29 Glycin-C-Acetyltransferase
2.3.1.30 Serin-O-Acetyltransferase
2.3.1.37 5-Aminolevulinatsynthase
2.3.1.39 [Acyl-Carrierprotein]-S-Malonyltransferase
2.3.1.41 3-Oxoacyl-[Acyl-Carrierprotein]-Synthase
2.3.1.46 Homoserin-O-Succinyltransferase
2.3.1.47 8-Amino-7-Oxononanoatsynthase
2.3.1.5 Arylamin-N-Acetyltransferase
2.3.1.51 1-Acylglycerol-3-phosphat-O-Acyltransferase
2.3.1.54 Format c-Acetyltransferase
2.3.1.57 Diamin-N-Acetyltransferase
2.3.1.61 Dihydrolipoamid-S-Succinyltransferase
2.3.1.74 Naringenin-Chalconsynthase
2.3.1.8 Phosphat-Acetyltransferase
2.3.1.9 Acetyl-CoA-C-Acetyltransferase
2.3.2.2 γ-Glutamyltransferase
2.3.2.6 Leucyltransferase
2.4.1.1 Phosphorylase
2.4.1.11 Glycogen(Stärke)synthase
2.4.1.12 Cellulosesynthase (UDP-bildend)
2.4.1.15 α,α-Trehalosephosphatsynthase (UDP-bildend)
2.4.1.18 1,4-α-Glucan-Verzweigungsenzym
2.4.1.182 Lipid-A-Disaccharidsynthase
2.4.1.21 Stärkesynthase
2.4.1.24 1,4-α-Glucan-6-α-Glucosyltransferase
2.4.1.25 4-α-Glucanotransferase
2.4.1.44 Lipopolysaccharid-Galactosyltransferase
2.4.1.58 Lipopolysaccharid-Glucosyltransferase I
2.4.1.7 Sucrosephsophorylase
2.4.1.83 Dolichylphosphat-β-D-Mannosyltransferase
2.4.2.1 Purin-Nucleosidphosphorylase
2.4.2.10 OrotATPhosphoribosyltransferase
2.4.2.11 NicotinATPhosphoribosyltransferase
2.4.2.14 Amidophosphoribosyltransferase
2.4.2.17 ATP-Phosphoribosyltransferase
2.4.2.18 AnthranilATPhosphoribosyltransferase
2.4.2.19 Nicotinat-Nucleotidpyrophosphorylase (carboxylierend)
2.4.2.2 Pyrimidin-Nucleosidphosphorylase
2.4.2.21 Nicotinat-Nucleotid-Dimethylbenzimidazol- Phosphoribosyl
2.4.2.22 Xanthinphosphoribosyltransferase
2.4.2.28 5'-Methylthioadenosinphosphorylase
2.4.2.29 Queuin-tRNA-Ribosyltransferase
2.4.2.3 Uridinphosphorylase
2.4.2.4 Thymidinphosphorylase
2.4.2.7 Adeninphosphoribosyltransferase
2.4.2.8 Hypoxanthinphosphoribosyltransferase
2.4.2.9 Uracilphosphoribosyltransferase
2.4.99.1 β-Galactosid-α-2,6-sialyltransferase
2.5.1.1 Dimethylallyltranstransferase
2.5.1.10 Geranyltranstransferase
2.5.1.15 Dihydropteroatsynthase
2.5.1.16 Spermidinsynthase
2.5.1.17 Cob(I)alamin-Adenosyltransferase
2.5.1.18 Glutathiontransferase
2.5.1.19 3-Phosphoshikimat-1-Carboxyvinyltransferase
2.5.1.22 Sperminsynthase
2.5.1.26 Alkylglyceronephosphatsynthase
2.5.1.29 Farnesyltranstransferase
2.5.1.3 Thiaminphosphat-Pyrophosphorylase
2.5.1.31 Di-trans,poly-cis-Decaprenylcistransferase
2.5.1.33 trans-Pentaprenyltranstransferase
2.5.1.6 Methionin-Adenosyltransferase
2.5.1.7 UDP-N-Acetylglucosamin-1-Carboxyvinyltransferase
2.5.1.8 tRNA-Isopentenyltransferase
2.5.1.9 Riboflavinsynthase
2.6.1.1 Aspartattransaminase
2.6.1.11 Acetylornithin-Transaminase
2.6.1.13 Ornithin-Oxosäure-Transaminase
2.6.1.14 Asparagin-Oxosäure-Transaminase
2.6.1.16 Glutamin-Fructose-6-phosphat-Transaminase (isomerisierend)
2.6.1.19 4-Aminobutyrat-Transaminase
2.6.1.2 Alanintransaminase
2.6.1.21 D-Alanintransaminase
2.6.1.42 Verzweigte-Ketten-Aminosäure-Transaminase
2.6.1.5 Tyrosintransaminase
2.6.1.52 Phosphoserintransaminase
2.6.1.57 Aromatische-Aminosäure-Transaminase
2.6.1.62 Adenosylmethionin-8-Amino-7-oxononanoat- Transaminase
2.6.1.64 Glutamin-Phenylpyruvat-Transaminase
2.6.1.66 Valin-Pyruvat-ransaminase
2.6.1.7 Kynurenin-Oxoglutarat-Transamininase
2.6.1.9 Histidinolphosphat-Transaminase
2.7.1.105 6-Phosphofructo-2-kinase
2.7.1.107 Diacylglycerolkinase
2.7.1.11 6-Phosphofructokinase
2.7.1.116 [Isocitratdehydrogenase (NADP+)]-kinase
2.7.1.12 Gluconokinase
2.7.1.15 Ribokinase
2.7.1.16 Ribulokinase
2.7.1.17 Xylulokinase
2.7.1.19 Phosphoribulokinase
2.7.1.2 Glucokinase
2.7.1.21 Thymidinkinase
2.7.1.25 Adenylylsulfatkinase
2.7.1.26 Riboflavinkinase
2.7.1.3 Ketohexokinase
2.7.1.30 Glycerolkinase
2.7.1.33 Pantothenatkinase
2.7.1.35 Pyridoxalkinase
2.7.1.37 Proteinkinase
2.7.1.39 Homoserinkinase
2.7.1.4 Fructokinase
2.7.1.40 Pyruvatkinase
2.7.1.45 2-Dehydro-3-desoxygluconokinase
2.7.1.47 D-Ribulokinase
2.7.1.48 Uridinkinase
2.7.1.49 Hydroxymethylpyrimidinkinase
2.7.1.5 Rhamnulokinase
2.7.1.50 Hydroxyethylthiazolkinase
2.7.1.52 Fucokinase
2.7.1.53 L-Xylulokinase
2.7.1.56 1-Phosphofructokinase
2.7.1.6 Galactokinase
2.7.1.69 Protein-N(pai)-Phosphohistidin-Zucker- Phosphotransferase
2.7.1.71 Shikimatkinase
2.7.1.73 Inosinkinase
2.7.2.1 Acetatkinase
2.7.2.11 Glutamat-5-kinase
2.7.2.2 Carbamatkinase
2.7.2.3 Phosphoglyceratkinase
2.7.2.4 Aspartatkinase
2.7.2.8 Acetylglutamatkinase
2.7.3.9 Phosphoenolpyruvat-Protein-Phosphotransferase
2.7.4.1 Polyphosphatkinase
2.7.4.14 Cytidylatkinase
2.7.4.16 Thiaminphosphatkinase
2.7.4.3 Adenylatkinase
2.7.4.6 Nucleosiddiphosphatkinase
2.7.4.8 Guanylatkinase
2.7.4.9 DTMP-Kinase
2.7.6.1 Ribosephosphat-Pyrophosphokinase
2.7.6.3 2-Amino-4-hydroxy-6-hydroxymethyldihydropteridin- Pyro-
2.7.6.5 GTP-Pyrophosphokinase
2.7.7.10 UTP-Hexose-1-phosphat-Uridylyltransferase
2.7.7.12 UDP-Glucose-Hexose-1-phosphat-Pyridylyltransferase
2.7.7.13 Mannose-1-phosphat-Guanylyltransferase
2.7.7.19 Polynucleotid-Adenylyltransferase
2.7.7.2 FMN-Adenylyltransferase
2.7.7.22 Mannose-1-phosphat-Guanylyltransferase (GDP)
2.7.7.24 Glucose-1-phosphat-Thymidylyltransferase
2.7.7.25 tRNA-Adenylyltransferase
2.7.7.27 Glucose-1-phosphat-Adenylyltransferase
2.7.7.38 3-Desoxy-manno-octulosonat-Cytidylyltransferase
2.7.7.39 Glycerol-3-phosphat-Cytidylyltransferase
2.7.7.4 Sulfatadenylyltransferase
2.7.7.41 Phosphatidat-Cytidylyltransferase
2.7.7.42 [Glutamat-Ammoniak-Ligase]-Adenylyltransferase
2.7.7.48 RNA-bezogene RNA-Polymerase
2.7.7.49 RNA-bezogene DNA-Polymerase
2.7.7.56 tRNA-Nucleotidyltransferase
2.7.7.59 [Protein-PII]-Uridylyltransferase
2.7.7.6 DNA-bezogene RNA-Polymerase
2.7.7.7 DNA-bezogene DNA-Polymerase
2.7.7.8 Polyribonucleotid-Nucleotidyltransferase
2.7.7.9 UTP-Glucose-1-phosphat-Uridylyltransferase
2.7.8.13 Phospho-N-Acetylmuramoyl-Pentapeptidtransferase
2.7.8.15 UDP-N-Acetylglucosamin-Dolichyl-
2.7.8.20 Phosphatidylglycerol-Membranoligosaccharid
2.7.8.5 CDP-Diacylglycerol-Glycerol-3-phsophat-3- phosphatidyltransferase
2.7.8.6 Undecaprenylphosphat-Galactosephosphotransferase
2.7.8.8 CDP-Diacylglycerol-Serin-O-Phosphatidyltransferase
2.7.9.2 Pyruvat-Wasser-Dikinase
2.8.1.1 Thiosulfat-Schwefeltransferase
2.8.1.5 Thiosulfatdithiol-Schwefeltransferase 2.8.3.5 3-Oxosäure-CoA-Transferase
2.8.3.6 3-Oxoadipat-CoA-Transferase
2.8.3.9 Butyrat-Acetoacetat-CoA-Transferase
2.9.1.1 L-Seryl-tRNA(Ser)-Selentransferase
3.1.1.1 Carboxylesterase
3.1.1.10 Tropinesterase
3.1.1.11 Pectinesterase
3.1.1.2 Arylesterase
3.1.1.29 Aminoacyl-tBNA-Hydrolase
3.1.1.3 Triacylglycerol-Lipase
3.1.1.32 Phospholipase A1
3.1.1.45 Carboxymethylenbutenlipase
3.1.1.5 Lisophospholipase
3.1.1.61 Protein-Glutamat-Methylesterase
3.1.11.1 Exodesoxyribonuclease I
3.1.11.2 Exodesoxyribonuclease III
3.1.11.5 Exodesoxyribonuclease V
3.1.11.6 Exodesoxyribonuclease VII
3.1.13.1 Exoribonuclease II
3.1.2.1 Acetyl-CoA-Hydrolase
3.1.2.2 Palmitoyl-CoA-Hydrolase
3.1.2.6 Hydroxyacylglutathionhydrolase
3.1.21.1 Desoxyribonuclease I
3.1.21.2 Desoxyribonuclease IV (Phage-T4-induziert)
3.1.21.3 Typ I sequenzspezifische Desoxyribonuclease
3.1.22.4 Crossover-Junction Endoribonuclease
3.1.25.1 Desoxyribonuclease (Pyrimidindimer)
3.1.26.3 Ribonuclease III
3.1.26.4 Kälberthymus-Ribonuclease H
3.1.26.5 Ribonuclease P
3.1.27.6 Enterobacter-Ribonuclease
3.1.3.1 alkalische Phosphatase
3.1.3.10 Glucose-1-phosphatase
3.1.3.11 Fructosebisphosphatase
3.1.3.12 Trehalosephosphatase
3.1.3.15 Histidinolphosphatase
3.1.3.16 Phosphoprotein-Phosphatase
3.1.3.18 Phosphoglycolat-Phosphatase
3.1.3.2 saure Phosphatase
3.1.3.25 Myoinositol-1(oder 4)-monophosphatase
3.1.3.27 Phosphatidylglycerophosphatase
3.1.3.3 Phosphoserinphosphatase
3.1.3.41 4-Nitrophenylphosphatase
3.1.3.46 Fructose-2,6-bisphosphat-2-phosphatase
3.1.3.48 Protein-Tyrosin-Phosphatase
3.1.3.5 5'-Nucleotidase
3.1.4.14 [Acyl-Carrierprotein]-Phosphodiesterase
3.1.4.16 2',3'-zyklisches Nucleotid-2'-Phosphodiesterase
3.1.4.17 3',5'-zyklisches Nucleotid-Phosphodiesterase
3.1.4.46 Glycerophosphodiester-Phosphodiesterase
3.1.5.1 dGTPase
3.1.6.1 Arylsulfatase
3.1.6.13 Iduronat-2-sulfatase
3.1.6.14 N-Acetylglucosamin-6-sulfatase
3.1.6.2 Sterylsulfatase
3.1.6.4 N-Acetylgalactosamin-6-sulfatase
3.1.6.8 Cerebrosidsulfatase
3.1.7.2 Guanosin-3',5'-bis(diphosphat) 3'-pyrophosphatase
3.2.1.1 α-Amylase
3.2.1.10 Oligo-1,6-glucosidase
3.2.1.135 Neopullulanase
3.2.1.14 Chitinase
3.2.1.17 Lysozym
3.2.1.20 α-Glucosidase
3.2.1.21 β-Glucosidase
3.2.1.22 α-Galactosidase
3.2.1.23 β-Galactosidase
3.2.1.24 α-Mannosidase
3.2.1.28 α,α-Trehalase
3.2.1.3 Glucan-1,4-α-Glucosidase
3.2.1.31 β-Glucuronidase
3.2.1.37 Xylan-1,4-β-xylosidase
3.2.1.48 Sucrose-α-glucosidase
3.2.1.54 Cyclomaltodextrinase
3.2.1.68 Isoamylase
3.2.1.70 Glucan-1,6-α-glucosidase
3.2.1.74 Glucan-1,4-β-glucosidase
3.2.1.8 Endo-1,4-β-Xylanase
3.2.1.86 6-Phospho-β-glucosidase
3.2.1.93 α,α-Phosphotrehalase
3.2.2.20 DNA-3-Methyladeninglycosidase I
3.2.2.21 DNA-3-Methyladeninglycosidase II
3.2.2.23 Formamidopyrimidin-DNA-Glycosidase
3.2.2.24 ADP-Ribosyl-[Distickstoffreductase]-hydrolase
3.2.2.4 AMP-Nucleosidase
3.3.2.1 Isochorismatase
3.3.2.3 Epoxidhydrolase
3.4.11.1 Leucyl-Aminopeptidase
3.4.11.10 bakterielle Leucyl-Aminopeptidase
3.4.11.15 Lysyl-Aminopeptidase
3.4.11.18 Methionyl-Aminopeptidase
3.4.11.2 Membran-Alanyl-Aminopeptidase
3.4.11.5 Prolyl-Aminopeptidase
3.4.11.7 Glutamyl-Aminopeptidase
3.4.11.9 X-Pro-Aminopeptidase
3.4.13.3 X-His-Dipeptidase
3.4.13.9 X-Pro-Dipeptidase DCP
3.4.16.4 Serin-Typ D-Ala,D-Ala-Carboxypeptidase
3.4.19.1 Acylaminoacylpeptidase
3.4.21.26 Prolyloligopeptidase
3.4.21.53 Endopeptidase Ia
3.4.21.83 Oligopeptidase B
3.4.21.87 Omptin
3.4.21.92 Endopeptidase CLP
3.4.23.36 Signalpeptidase II
3.4.24.13 IgA-spezifische Metallendopeptidase
3.4.24.15 Thimetoligopeptidase
3.4.24.57 O-Syaloglycoproteinendopeptidase
3.4.99.36 Leitpeptidase
3.4.99.41 mitochondriale Prozessierungs-Peptidase
3.4.99.44 Pitrilysin
3.4.99.45 Insulinase
3.5.1.1 Asparaginase
3.5.1.10 Formyltetrahydrofolatdeformylase
3.5.1.11 Penicillin-Amidase
3.5.1.13 Aryl-Acylamidase
3.5.1.14 Aminoacylase
3.5.1.16 Acetylornithindeacetylase
3.5.1.18 Succinyl-Diaminopimelatdesuccinylase
3.5.1.2 Glutaminase
3.5.1.25 N-Acetylglucosamin-6-phosphatdeacetylase
3.5.1.26 N4-(β-N-Acetylglucosaminyl)-L-Asparaginase
3.5.1.27 N-Formylmethionylaminoacyl-tRNA-Deformylase
3.5.1.28 N-Acetylmuramoyl-L-Alanin-Amidase
3.5.1.32 Hippurathydrolase
3.5.1.38 Glutamin-(Asparagin-)ase
3.5.1.59 N-Carbamoylsarcosin-Amidase
3.5.2.2 Dihydropyrimidinase
3.5.2.3 Dihydroorotase
3.5.2.5 Allantoinase
3.5.2.6 β-Lactamase
3.5.3.1 Arginase
3.5.3.11 Agmatinase
3.5.3.19 Ureidoglycolathydrolase
3.5.4.1 Cytosindesaminase
3.5.4.10 IMP-Cyclohydrolase
3.5.4.13 dCTP-Deaminase
3.5.4.16 GTP-Cyclohydrolase I
3.5.4.19 Phosphoribosyl-AMP-Cyclohydrolase
3.5.4.2 Adenindeaminase
3.5.4.25 GTP-Cyclohydrolase II
3.5.4.4 Adenosindesaminase
3.5.4.5 Cytidindesaminase
3.5.4.9 Methenyltetrahydrofolatcyclohydrolase
3.6.1.1 anorganische Pyrophosphatase
3.6.1.11 Exopolyphosphatase
3.6.1.23 dUTP-Pyrophosphatase
3.6.1.26 CDP-Diacylglycerol-Pyrophosphatase
3.6.1.31 Phosphoribosyl-ATP-Pyrophosphatase
3.6.1.32 Myosin-ATPase
3.6.1.34 H+-transportierende ATP-Synthase
3.6.1.35 H+-transportierende ATPase
3.6.1.36 H+/K+ austauschende ATPase
3.6.1.37 Na+/K+ austauschende ATPase
3.6.1.38 Ca2+-transportierende ATPase
3.6.1.41 bis(5'-Nucleosyl)-tetraphosphatase (symmetrisch)
3.6.1.45 UDP-Zuckerdiphosphatase
3.6.1.7 Acylphosphatase
3.7.1.8 2,6-Dioxo-6-phenylhexα-3-enoathydrolase
3.7.1.9 2-Hydroxymuconatsemialdehydhydrolase
3.8.1.3 Haloacetatdehalogenase
3.8.1.6 4-Chlorobenzoatdehalogenase
4.1.1.11 Aspartat-1-decarboxylase
4.1.1.15 Glutamatdecarboxylase
4.1.1.17 Ornithindecarboxylase
4.1.1.18 Lysindecarboxylase
4.1.1.19 Arginindecarboxylase
4.1.1.20 Diaminopimelatdecarboxylase
4.1.1.21 Phosphoribosylaminoimidazolcarboxylase
4.1.1.23 Orotidin-5'-phosphatdecarboxylase
4.1.1.3 Oxaloacetatdecarboxylase
4.1.1.31 Phosphoenolpyruvatcarboxylase
4.1.1.37 Uroporphyrinogendecarboxylase
4.1.1.41 Methylmalonyl-CoA-Decarboxylase
4.1.1.47 Tartronatsemialdehydsynthase
4.1.1.48 Indol-3-glycerolphosphatsynthase
4.1.1.49 Phosphoenolpyruvatcarboxykinase (ATP)
4.1.1.50 Adenosylmethionindecarboxylase
4.1.1.64 2,2-Dialkylglycindecarboxylase (Pyruvat)
4.1.1.65 Phosphatidylserindecarboxylase
4.1.1.71 2-Oxoglutaratdecarboxylase
4.1.1.73 Tartratdecarboxylase
4.1.1.8 Oxalyl-CoA-Decarboxylase
4.1.2.13 Fructosebisphosphataldolase
4.1.2.14 2-Dehydro-3-desoxyphosphogluconat-Aldolase
4.1.2.15 2-Dehydro-3-desoxyphosphoheptonat-Aldolase
4.1.2.16 2-Dehydro-3-desoxyphosphooctonat-Aldolase
4.1.2.17 L-Fuculosephosphataldolase
4.1.2.19 Rhamnulose-1-phosphat-Aldolase
4.1.2.25 Dihydroneopterinaldolase
4.1.2.4 Desoxyribosephosphataldolase
4.1.3. 1 Isocitratlyase
4.1.3.12 2-Isopropylmalatsynthase
4.1.3.16 4-Hydroxy-2-oxoglutarataldolase
4.1.3.18 Acetolactatsynthase
4.1.3.2 Malatsynthase
4.1.3.27 Anthranilatsynthase
4.1.3.3 N-Acetylneuraminatlyase
4.1.3.36 Naphthoatsynthase
4.1.3.6 Citratlyase
4.1.3.7 Citrat(SI)synthase
4.1.99.1 Tryptophanase
4.1.99.2 Tyrosin-Phenollyase
4.1.99.3 Desoxyribodipyrimidinphotolyase
4.1.99.4 1-Aminocyclopropan-1-carboxylatdeaminase
4.2.1.1 Carbonatdehydratase
4.2.1.10 3-Dehydrochinatdehydratase
4.2.1.11 Phosphopyruvathydratase
4.2.1.12 Phosphogluconatdehydratase
4.2.1.13 L-Serindehydratase
4.2.1.14 D-Serindehydratase
4.2.1.16 Threonindehydratase
4.2.1.17 Enoyl-CoA-Hydratase
4.2.1.19 Imidazolglycerolphosphatdehydratase
4.2.1.2 Fumarathydratase
4.2.1.20 Tryptophansynthase
4.2.1.24 Porphobilinogensynthase
4.2.1.3 Aconitathydratase
4.2.1.32 L(+)-Tartratdehydratase
4.2.1.33 3-Isopropylmalatdehydratase
4.2.1.36 Homoaconitathydratase
4.2.1.40 Glucaratdehydratase
4.2.1.46 dTDP-Glucose-4,6-dehydratase
4.2.1.51 Prephenatdehydratase
4.2.1.52 Dihydrodipicolinatsynthase
4.2.1.60 3-Hydroxydecanoyl-[Acyl-Carrierprotein]- dehydratase
4.2.1.7 Altronatdehydratase
4.2.1.74 lange Ketten-enoyl-CoA-Hydratase
4.2.1.75 Uroporphyrinogen-III-Synthase
4.2.1.8 Mannonatdehydratase
4.2.1.89 Carnitindehydratase
4.2.1.9 Dihydroxysäuredehydratase
4.2.99.11 Methylglyoxalsynthase
4.2.99.18 DNA-(apurinische oder apyrimidinische Sequenz)- lyase
4.2.99.2 Threoninsynthase
4.2.99.8 Cysteinsynthase
4.2.99.9 O-Succinylhomoserin-(Thiol)-lyase
4.3.1.1 Aspartat-Ammoniaklyase
4.3.1.7 Ethanolamin-Ammoniaklyase
4.3.1.8 Hydroxymethylbilansynthase
4.3.2.1 Argininosuccinatlyase
4.3.2.2 Adenylsuccinatlyase
4.3.99.1 Cyanatlyase
4.4.1.1 Cystathionin-y-lyase
4.4.1.11 Methionin-7-lyase
4.4.1.14 1-Aminocyclopropan-1-carboxylatsynthase
4.4.1.5 Lactoylglutathionlyase
4.4.1.8 Cystathionin-β-lyase
4.5.1.5 S-Carboxymethylcysteinsynthase
4.6.1.1 Adenylatcyclase
4.6.1.10 6-Pyruvoiltetrahydrobiopterinsynthase
4.6.1.3 3-Dehydrochinatsynthase
4.6.1.4 Chorismatsynthase
4.99.1.1 Ferrochelatase
5.1.1.1 Alaninracemase
5.1.1.11 Phenylalaninracemase (ATP-hydrolysierend)
5.1.1.13 Aspartatracemase
5.1.1.3 Glutamatracemase
5.1.1.7 Diaminopimaratepimerase
5.1.2.2 Mandelatracemase
5.1.3.1 Ribulosephosphat-3-epimerase
5.1.3.13 dTDP-4-Dehydrorhamnose 3,5-epimerase
5.1.3.14 UDP-N-Acetylglucosamin.2-epimerase
5.1.3.2 UDP-Glucose.4-epimerase
5.1.3.3 Aldose.1-epimerase
5.1.3.4 L-Ribulosephosphat-4-epimerase
5.2.1.8 Peptidylprolylisomerase
5.3.1.1 Triosephosphatisomerase
5.3.1.10 Glucosamin-6-phosphatisomerase
5.3.1.12 Glucuronatisomerase
5.3.1.14 L-Rhamnoseisomerase
5.3.1.16 N-(5'-Phospho-D-ribosylformimino)-5-amino-1-
5.3.1.17 4-Desoxy-L-threo-5-hexosulose-uronatketoisomerase
5.3.1.24 Phosphoribosylanthranilatisomerase
5.3.1.4 L-Arabinoseisomerase
5.3.1.5 Xyloseisomerase
5.3.1.6 Ribose-5-phosphatepimerase
5.3.1.8 Mannose-6-phosphatisomerase
5.3.1.9 Glucose-6-phosphatisomerase
5.3.3.1 Steroid-Δ-isomerase
5.3.3.10 5-Carboxymethyl-2-hydroxymuconat-Δ-isomerase
5.3.3.2 Isopentenyldiphosphat-Δ-isomerase
5.3.3.8 Dodecenoyl-CoA-Δ-isomerase
5.3.4.1 Proteindisulfidisomerase
5.4.2.1 Phosphoglyceratmutase
5.4.2.2 Phosphoglucomutase
5.4.2.6 β-Phosphoglucomutase
5.4.2.7 Phosphopentomutase
5.4.2.8 Phosphomannomutase
5.4.2.9 Phosphoenolpyruvatmutase
5.4.3.8 Glutamat-1-semialdehyd-2,1-aminomutase
5.4.99.12 tRNA-Pseudouridinsynthase I
5.4.99.2 Methylmalonyl-CoA-Mutase
5.4.99.5 Chorismatmutase
5.4.99.6 Isochorismatsynthase
5.5.1.1 Muconatcycloisomerase
5.5.1.2 3-Carboxy-cis,cis-muconatcycloisomerase
5.5.1.7 Chloromuconatcycloisomerase
5.99.1.2 DNA-Topoisomerase
5.99.1.3 DNA-Topoisomerase (ATP-hydrolysierend)
6.1.1.1 Tyrosin-tRNA-Ligase
6.1.1.10 Methionin-tRNA-Ligase
6.1.1.11 Serin-tPKA-Ligase
6.1.1.12 Aspartat-tRNA-Ligase
6.1.1.14 Glycin-tRNA-Ligase
6.1.1.15 Prolin-tRNA-Ligase
6.1.1.16 Cystein-tRNA-Ligase
6.1.1.17 Glutamat-tRNA-Ligase
6.1.1.18 Glutamin-tRNA-Ligase
6.1.1.19 Arginin-tHNA-Ligase
6.1.1.2 Tryptophan-tRNA-Ligase
6.1.1.20 Phenylalanin-tRNA-Ligase
6.1.1.21 Histidin-tRNA-Ligase
6.1.1.22 Asparagin-tRNA-Ligase
6.1.1.3 Threonin-LENA-Ligase
6.1.1.4 Leucin-tRNA-Ligase
6.1.1.5 Isoleucin-tRNA-Ligase
6.1.1.6 Lysin-tRNA-Ligase
6.1.1.7 Alanin-tRNA-Ligase
6.1.1.9 Valin-tRNA-Ligase
6.2.1.1 Acetat-CoA-Ligase
6.2.1.12 4-Coumarat-CoA-Ligase
6.2.1.22 Citrat(Pro-3S)-lyaseligase
6.2.1.26 O-Succinylbenzoat-CoA-Ligase
6.2.1.27 4-Hydroxybenzoat-CoA-Ligase
6.2.1.3 langkettige Fettsäure-CoA-Ligase
6.2.1.4 Succinat-CoA-Ligase (GTP-bildend)
6.2.1.5 Succinat-CoA-Ligase (ADP-bildend)
6.3.1.1 Aspartat-Ammoniak-Ligase
6.3.1.2 Glutamat-Ammoniak-Ligase
6.3.1.5 NAD+-Synthase
6.3.2.1 Pantoat-(β-Alaninligase
6.3.2.12 Dihydrofolatsynthase
6.3.2.13 UDP-N-Acetylmuramoylalnyl-D-glutamat-2,6- diaminopimelatligase
6.3.2.15 UDP-N-Acetylmuramoylalanyl-D-glutamyl-2,6-
6.3.2.17 Tetrahydrofolylpolyglutamatsynthase
6.3.2.19 Ubiquitin-Proteinligase
6.3.2.2 Glutamat-Cysteinligase
6.3.2.3 Glutathionsynthase
6.3.2.4 D-Alanin-D-Alaninligase
6.3.2.6 Phosphoribosylaminoimidazolsuccinocarboxyamidsynthase
6.3.2.8 UDP-N-Acetylmuramat-Alaninligase
6.3.2.9 UDP-N-Acetylmuramoylalanin-D-Glutamatligase
6.3.3.1 Phosphoribosyl-Formylglycinamidincycloligase
6.3.3.2 5-Formyltetrahydrofolatcycloligase
6.3.3.3 Dethiobiotinsynthase
6.3.4.13 Phosphoribosylamin-Glycinligase
6.3.4.14 Biotincarboxylase
6.3.4.15 Biotin-[Acetyl-CoA-carboxylase]-ligase
6.3.4.2 CTP-Synthase
6.3.4.4 Adenylosuccinatsynthase
6.3.4.5 Argininosuccinatsynthase
6.3.4.6 Harnstoffcarboxylase
6.3.5.1 NAD+-Synthase (Glutamin-hydrolysierend)
6.3.5.2 GMP-Synthase (Glutamin-hydrolysierend)
6.3.5.3 Phosphoribosylformylglycinamidinsynthase
6.3.5.4 Asparaginsynthase (Glutamin-hydrolysierend)
6.3.5.5 Carbamoylphosphatsynthase (Glutamin-hydrolysierend)
6.4.1.2 Acetyl-CoA-Carboxylase
6.4.1.3 Propionyl-CoA-Carboxylase
6.5.1.2 DNA-Ligase (NAD+). Enzyme aus Corynebacteriurn glutamicum mit EC-Nummer 1.1.1.1 Alkoholdehydrogenase
1.1.1.100 3-Oxoacyl-[Acyl-Carrierprotein]-reductase
1.1.1.105 Retinoldehydrogenase
1.1.1.133 dTDP-4-Dehydrorhamnosereductase
1.1.1.158 UDP-N-Acetylmuramatdehydrogenase
1.1.1.169 2-Dehydropantoat-2-reductase
1.1.1.17 Mannitol-1-phosphat-5-dehydrogenase
1.1.1.18 Myoinositol-2-dehydrogenase
1.1.1.195 Cinnamyl-Alkoholdehydrogenase
1.1.1.2 Alkoholdehydrogenase (NADP+)
1.1.1.205 IMP-Dehydrogenase
1.1.1.21 Aldehydreductase
1.1.1.218 Morphin-6-dehydrogenase
1.1.1.22 UDP-Glucose-6-dehydrogenase
1.1.1.23 Histidinoldehydrogenase
1.1.1.25 Shikimat-5-dehydrogenase
1.1.1.27 L-Lactatdehydrogenase
1.1.1.28 D-Lactatdehydrogenase
1.1.1.29 Glyceratdehydrogenase
1.1.1.3 Homoserindehydrogenase
1.1.1.36 Acetoacetyl-CoA-Reductase
1.1.1.37 Malatdehydrogenase
1.1.1.38 Malatdehydrogenase (Oxaloacetat-decarboxylierend)
1.1.1.4 (R,R)-Butandioldehydrogenase
1.1.1.42 Isocitratdehydrogenase (NADP+)
1.1.1.44 Phosphogluconatdehydrogenase (decarboxylierend)
1.1.1.47 Glucose-1-dehydrogenase
1.1.1.49 Glucose-6-phosphat 1-dehydrogenase
1.1.1.69 Gluconat-5-dehydrogenase
1.1.1.8 Glycerol-3-phosphatdehydrogenase (NAD+)
1.1.1.85 3-Isopropylmalatdehydrogenase
1.1.1.86 Ketosäure-Reductoisomerase
1.1.1.90 Aryl-Alkoholdehydrogenase
1.1.1.91 Aryl-Alkoholdehydrogenase (NADP+)
1.1.1.95 Phosphoglyceratdehydrogenase
1.1.2.3 L-Lactatdehydrogenase (Cytochrom)
1.1.2.4 D-Lactatdehydrogenase (Cytochrom)
1.1.3.15 (S)-2-Hydroxysäureoxidase
1.1.99.5 Glycerol-3-phosphatdehydrogenase
1.10.3.2 Laccase
1.11.1.12 Phospholipid-Hydroperoxid-Glutathionperoxidase
1.11.1.6 Katalase
1.11.1.9 Glutathionperoxidase
1.13.11.1 Catechol-1,2-dioxygenase
1.13.11.27 4-Hydroxyphenylpyruvatdioxygenase
1.13.11.32 2-Nitropropandioxygenase
1.13.11.37 Hydroxychinol 1,2-dioxygenase
1.14.12.3 Benzen-1,2-dioxygenase
1.14.13.1 Salicylat-1-monooxygenase
1.14.13.2 4-Hydroxybenzoat3-monooxygenase
1.14.13.20 2,4-Dichlorophenol-6-monooxygenase
1.14.13.7 Phenol-2-monooxygenase
1.14.13.8 Dimethylanilinmonooxygenase (N-Oxid-bildend)
1.14.14.3 Alkanalmonooxygenase (FMH-verknüpft)
1.14.99.3 Hämoxygenase (dezyklisierend)
1.15.1.1 Superoxiddismutase 1.16.1.1 Quecksilber(II)-Reductase
1.17.4.1 Ribonucleosiddiphosphatreductase
1.18.1.2 Ferredoxin-NADP+-Reductase
1.18.1.4 Rubredoxin-NAD(P)+-Reductase
1.2.1.1 Formaldehyddehydrogenase (Glutathion)
1.2.1.11 Aspartatsemialdehyddehydrogenase
1.2.1.12 Glyceraldehyd-3-phosphatdehydrogenase (phosphorylierend)
1.2.1.13 Glyceraldehyd-3-phosphatdehydrogenase (NADP+)(phosphorylierend)
1.2.1.16 Succinatsemialdehyddehydrogenase (NAD(P)+)
1.2.1.2 Formatdehydrogenase
1.2.1.22 Lactaldehyddehydrogenase
1.2.1.27 Methylmalonatsemialdehyddehydrogenase (acylierend)
1.2.1.3 Aldehyddehydrogenase (NAD+)
1.2.1.38 N-Acetyl-γy-glutamylphosphatreductase
1.2.1.39 Phenylacetaldehyddehydrogenase
1.2.1.41 Glutamat-5-semialdehyddehydrogenase
1.2.1.8 Betainaldehyddehydrogenase
1.2.2.2 Pyruvatdehydrogenase (Cytochrom)
1.2.3.3 Pyruvatoxidase
1.2.4.1 Pyruvatdehydrogenase (Lipoamid)
1.2.4.2 Oxoglutaratdehydrogenase (Lipoamid)
1.3.1.12 Prephenatdehydrogenase
1.3.1.26 Dihydrodipicolinatreductase
1.3.1.28 2,3-Dihydro-2,3-dihydroxybenzoatdshydrogenase
1.3.1.32 Maleylacetatreductase
1.3.1.42 12-Oxophytodienoatreductase
1.3.1.45 2'-Hydroxyisoflavonereductase
1.3.3.1 Dihydroorotatoxidase
1.3.3.3 Coproporphyrinogenoxidase
1.3.3.4 Protoporphyrinogenoxidase
1.3.3.5 Bilirubinoxidase
1.3.5.1 Succinatdehydrogenase (Ubichinon)
1.3.99.1 Succinatdehydrogenase
1.3.99.3 Acyl-CoA-Dehydrogenase
1.3.99.4 3-Oxosteroid-1-dehydrogenase
1.3.99.5 3-Oxo-5α-steroid-4-dehydrogenase
1.4.1.13 Glutamatsynthase (NADPH)
1.4.1.14 Glutamatsynthase (NADH)
1.4.1.16 Diaminopimelatdehydrogenase
1.4.1.4 Glutamatdehydrogenase (NADP+)
1.4.99.1 D-Aminosäuredehydrogenase
1.5.1.12 1-Pyrrolin-5-carboxylatdehydrogenase
1.5.1.15 Methylenetetrahydrofolatdehydrogenase (NAD+)
1.5.1.2 Pyrrolin-5-carboxylatreductase
1.5.1.20 Methylenetetrahydrofolatreductase (NADPH)
1.5.1.3 Dihydrofolatreductase
1.5.1.5 Methylentetrahydrofolatdehydrogenase (NADP+)
1.5.3.1 Sarcosinoxidase
1.5.99.8 Prolindehydrogenase
1.6.4.2 Glutathionreductase (NADPH)
1.6.4.5 Thioredoxinreductase (NADPH)
1.6.5.3 NADH-Dehydrogenase (Ubichinon)
1.6.5.5 NADPH : Chinonreductase
1.6.8.2 NADPH-Dehydrogenase (Flavin)
1.6.99.1 NADPH-Dehydrogenase
1.7.7.1 Ferredoxin-Nitritreductase
1.7.99.4 Nitratreductase
1.7.99.5 5,10-Methylentetrahydrofolatreductase (FADH2)
1.8.1.4 Dihydrolipoamiddehydrogenase
1.8.4.6 Protein-Methionin-S-oxidreductase
1.8.99.4
1.9.3.1 Cytochrom c-Oxidase
2.1.1.107 Uroporphyrin-III C-Methyltransferase
2.1.1.13 5-Methyltetrahydrofolat-Homocystein S- methyltransferase
2.1.1.14 5-Methyltetrahydropteroyltriglutamat-Homocystein
2.1.1.31 tRNA-(Guanin-N1-)-methyltransferase
2.1.1.37 DNA (Cytosin-5-)-methyltransferase
2.1.1.41 24-Sterol c-methyltransferase
2.1.1.45 Thymidylatsynthase
2.1.1.48 rRNA-(Adenin-N6-)-methyltransferase
2.1.1.63 Methylierte-DNA-Protein-Cystein-S- Methyltransferase
2.1.1.72 Sequenz-spezifische DNA-Methyltransferase (Adenin- spezifisch)
2.1.1.73 Sequenz-spezifische DNA-Methyltransferase (Cytosin-spezifisch)
2.1.1.79 Cyclopropan-Fettsäure-Acyl-Phospholipidsynthase
2.1.2.1 Glycinhydroxymethyltransferase
2.1.2.11 3-Methyl-2-oxobutanoathydroxymethyltransferase
2.1.2.2 Phosphoribosylglycinamidf-Formyltransferase
2.1.2.3 Phosphoribosylaminoimidazolcarboxamid- Formyltransferase
2.1.2.9 Methionyl-tRNA-Formyltransferase
2.1.3.2 Aspartatcarbamoyltransferase
2.1.3.3 Ornithincarbamoyltransferase
2.2.1.1 Transketolase
2.2.1.2 Transaldolase
2.3.1.128 ribosomales Protein-Alanin-N-Acetyltransferase
2.3.1.18 Galactosid-O-Acetyltransferase
2.3.1.28 Chloramphenicol-O-Acetyltransferase
2.3.1.30 Serin-O-Acetyltransferase
2.3.1.31 Homoserin-O-Acetyltransferase
2.3.1.35 Glutamat-N-Acetyltransferase
2.3.1.51 1-Acylglycerol-3-phosphat-O-Acyltransferase
2.3.1.61 Dihydrolipoamid-S-Succinyltransferase
2.3.1.8 Phosphat-Acetyltransferase
2.3.1.85 Fettsäuresynthase
2.3.2.2 γ-Glutamyltransferase
2.3.2.5 Glutaminyl-Peptid-Cyclotransferase
2.4.1.1 Phosphorylase
2.4.1.11 Glycogen(Stärke)synthase
2.4.1.15 α,α-Trehalosephosphatsynthase (UDP-bildend)
2.4.1.17 Glucuronosyltransferase
2.4.1.18 1,4-α-Glucan verzweigendes Enzym
2.4.1.19 Cyclomaltodextrin-Glucanotransferase
2.4.1.24 1,4-α-Glucan-6-α-glucosyltransferase
2.4.1.25 4-α-Glucanotransferase
2.4.1.52 Poly(glycerolphosphat)-α-glucosyltransferase
2.4.1.83 Dolichylphosphat-β-D-mannosyltransferase
2.4.2.10 Orotat-Phosphoribosyltransferase
2.4.2.14 Amidophosphoribosyltransferase
2.4.2.17 ATP-Phosphoribosyltransferase
2.4.2.18 Anthranilat-Phosphoribosyltransferase
2.4.2.19 Nicotinat-Nucleotid-Pyrophosphorylase (carboxylierend)
2.4.2.29 Queuin-tRNA-Ribosyltransferase
2.4.2.7 Adenin-Phosphoribosyltransferase
2.4.2.8 Hypoxanthin-Phosphoribosyltransferase
2.4.2.9 Uracil-Phosphoribosyltransferase
2.5.1.1 Dimethylallyltranstransferase
2.5.1.10 Geranyltranstransferase
2.5.1.15 Dihydropteroatsynthase
2.5.1.16 Spermidinsynthase
2.5.1.19 3-Phosphoshikimat-1-carboxyvinyltransferase
2.5.1.26 Alkylglyceronphosphatsynthase
2.5.1.29 Farnesyltranstransferase
2.5.1.3 Thiaminphosphatpyrophosphorylase
2.5.1.31 Di-trans,poly-cis-decaprenylcistransferase
2.5.1.6 Methionin-Adenosyltransferase
2.5.1.7 UDP-N-Acetylglucosamin-1-carboxyvinyltransferase
2.5.1.8 tRNA-Isopentenyltransferase
2.5.1.9 Riboflavinsynthase
2.6.1.1 Aspartattransaminase
2.6.1.11 Acetylornithintransaminase
2.6.1.13 Ornithin-Oxosäure-Transaminase
2.6.1.14 Asparagin-Oxosäure-Transaminase
2.6.1.16 Glutamin-Fructose-6-phosphattransaminase (isomerisierend)
2.6.1.18 β-Alanin-Pyruvattransaminase
2.6.1.19 4-Aminobutyrattransaminase
2.6.1.21 D-Alanintransaminase
2.6.1.52 Phosphoserintransaminase
2.6.1.62 Adenosylmethionin-8-amino-7- oxononanoattransaminase
2.6.1.64 Glutaminphenylpyruvattransaminase
2.6.1.7 Kynurenin-Oxoglutarattransaminase
2.6.1.9 Histidinolphosphattransaminase
2.7.1.11 6-Phosphofructokinase
2.7.1.12 Gluconokinase
2.7.1.15 Ribokinase
2.7.1.17 Xylulokinase
2.7.1.25 Adenylylsulfatkinase
2.7.1.26 Riboflavinkinase
2.7.1.30 Glycerolkinase
2.7.1.33 Pantothenatkinase
2.7.1.35 Pyridoxalkinase
2.7.1.37 Proteinkinase
2.7.1.39 Homoserinkinase
2.7.1.4 Fructokinase
2.7.1.40 Pyruvatkinase
2.7.1.49 Hydroxymethylpyrimidinkinase
2.7.1.56 1-Phosphofructokinase
2.7.1.69 Protein-N(pai)-Phosphohistidin-Zucker- Phosphotransferase
2.7.1.71 Shikimatkinase
2.7.2.1 Acetatkinase
2.7.2.11 Glutamat-5-kinase
2.7.2.3 Phosphoglyceratkinase
2.7.2.4 Aspartatkinase
2.7.2.8 Acetylglutamatkinase
2.7.3.9 Phosphoenolpyruvat-Protein-Phosphotransferase
2.7.4.14 Cytidylatkinase
2.7.4.16 Thiaminphosphatkinase
2.7.4.3 Adenylatkinase
2.7.4.6 Nucleosiddiphosphatkinase
2.7.4.8 Guanylatkinase
2.7.6.1 Ribosephosphatpyrophosphokinase
2.7.6.3 2-Amino-4-hydroxy-6-hydroxymethyldihydropteridin- Pyro-
2.7.6.5 GTP-Pyrophosphokinase
2.7.7.13 Mannose-1-phosphat-Guanylyltransferase
2.7.7.2 FMN-Adenylyltransferase
2.7.7.24 Glucose-1-phosphat-Thymidylyltransferase
2.7.7.25 tRNA-Adenylyltransferase
2.7.7.27 Glucose-1-phosphat-Adenylyltransferase
2.7.7.4 Sulfat-Adenylyltransferase
2.7.7.41 Phosphatidat-Cytidylyltransferase
2.7.7.42 [Glutamat-Ammoniak-Ligase]-Adenylyltransferase
2.7.7.56 tRNA-Nucleotidyltransferase
2.7.7.59 [Protein PII]-Uridylyltransferase
2.7.7.6 DNA-bezogene RNA-Polymerase
2.7.7.7 DNA-bezogene DNA-Polymerase
2.7.7.8 Polyribonucleotid-Nucleotidyltransferase
2.7.7.9 UTP-Glucose-1-phosphat-Uridylyltransferase
2.7.8.13 Phospho-N-Acetylmuramoyl-Pentapeptidtransferase
2.7.8.5 CDP-Diacylglycerol-glycerol-3-phsophat-3- phosphatidyltransferase
2.7.8.6 Undecaprenylphosphat-Galactosephosphotransferase
2.7.8.7 Holo-[Acyl-Carrierprotein]-synthase
2.7.9.2 Pyruvat-Wasser-Dikinase
2.8.1.1 Thiosulfat-Schwefeltransferase
3.1.1.1 Carboxylesterase
3.1.1.29 Aminoacyl-tRNA-Hydrolase
3.1.1.3 Triacylglycerollipase
3.1.1.59 Jugend-Hormonesterase
3.1.1.8 Cholinesterase
3.1.11.2 Exodesoxyribonuclease III
3.1.11.6 Exodesoxyribonuclease VII
3.1.13.1 Exoribonuclease II
3.1.2.1 Acetyl-CoA-Hydrolase
3.1.2.2 Palmitoyl-CoA-Hydrolase
3.1.2.6 Hydroxyacylglutathionhydrolase
3.1.21.3 TypI Sequenz-spezifische Desoxyribonuclease
3.1.21.5 Typ III Sequenz-spezifische Desoxyribonuclease
3.1.22.4 Crossover-Junction Endoribonuclease
3.1.26.3 Ribonuclease III
3.1.26.4 Kälberthymus-Ribonuclease H
3.1.26.5 Ribonuclease P
3.1.3.1 alkalische Phosphatase
3.1.3.12 Trehalosephosphatase
3.1.3.15 Histidinolphosphatase
3.1.3.16 Phosphoproteinphosphatase
3.1.3.18 Phosphoglycolatphosphatase
3.1.3.2 saure Phosphatase
3.1.3.25 Myoinositol-1(oder 4)-monophosphatase
3.1.3.3 Phosphoserinphosphatase
3.1.3.48 Protein-Tyrosinphosphatase
3.1.3.5 5'-Nucleotidase
3.1.4.46 Glycerophosphodiester-Phosphodiesterase
3.1.5.1 dGTPase
3.1.6.1 Arylsulfatase
3.1.7.2 Guanosin-3',5'-bis(diphosphat) 3'-pyrophosphatase
3.2.1.1 α-Amylase
3.2.1.10 Oligo-1,6-glucosidase
3.2.1.135 Neopullulanase
3.2.1.18 Exo-α-Sialidase
3.2.1.20 α-Glucosidase
3.2.1.21 β-Glucosidase
3.2.1.26 β-Fructofuranosidase
3.2.1.37 Xylan-1,4-β-xylosidase
3.2.1.41 α-Dextrin-endo-1,6-α-glucosidase
3.2.1.48 Sucrose-α-glucosidase
3.2.1.54 Cyclomaltodextrinase
3.2.1.58 Glucan-1,3-β-glucosidase
3.2.1.68 Isoamylase
3.2.1.7 Inulinase
3.2.1.74 Glucan-1,4-β-glucosidase
3.2.1.86 6-Phospho-β-glucosidase
3.2.1.93 α,α-Phosphotrehalase
3.2.2.20 DNA-3-MethyladeninglycosidaseI
3.2.2.23 Formamidopyrimidin-DNA-Glycosidase
3.2.2.4 AMP-Nucleosidase
3.3.1.1 Adenosylhomocysteinase
3.3.2.3 Epoxidhydrolase
3.4.11.15 Lysyl-Aminopeptidase
3.4.11.18 Methionyl-Aminopeptidase
3.4.11.2 Membran-Alanyl-Aminopeptidase
3.4.11.5 Prolyl-Aminopeptidase
3.4.13.9 X-Pro-Dipeptidase DCP
3.4.16.4 Serin-Typ D-Ala,D-Ala-Carboxypeptidase
3.4.17.4 Gly-X-Carboxypeptidase
3.4.21.1 Chymotrypsin
3.4.21.26 Prolyloligopeptidase
3.4.21.4 Trypsin
3.4.21.62 Subtilisin
3.4.21.66 Thermitase
3.4.21.83 Oligopeptidase B
3.4.23.36 Signalpeptidase II
3.4.24.11 Neprilysin
3.4.24.37 Saccharolysin
3.4.24.57 O-Syaloglycoproteinendopeptidase
3.4.99.36 Leitpeptidase
3.5.1.1 Asparaginase
3.5.1.10 Formyltetrahydrofolatdeformylase
3.5.1.13 Aryl-Acylamidase
3.5.1.14 Aminoacylase
3.5.1.16 Acetylornithindeacetylase
3.5.1.18 Succinyl-Diaminopimelatdesuccinylase
3.5.1.2 Glutaminase
3.5.1.27 N-Formylmethionylaminoacyl-tRNA-Deformylase
3.5.1.28 N-Acetylmuramoyl-L-Alaninamidase
3.5.1.32 Hippurathydrolase
3.5.1.38 Glutamin-(Asparagin-)ase
3.5.1.4 Amidase
3.5.1.5 Urease
3.5.2.12 6-Aminohexanoat-zyklisches Dimer-Hydrolase
3.5.2.3 Dihydroorotase
3.5.4.10 IMP-Cyclohydrolase
3.5.4.13 dCTP-Deaminase
3.5.4.16 GTP-Cyclohydrolase I
3.5.4.19 Phosphoribosyl-AMP-Cyclohydrolase
3.5.4.25 GTP-Cyclohydrolase II
3.5.4.9 Methenyltetrahydrofolatcyclohydrolase
3.6.1.1 anorganische Pyrophosphatase
3.6.1.11 Exopolyphosphatase
3.6.1.23 dUTP-Pyrophosphatase
3.6.1.3 Adenosintriphosphatase
3.6.1.31 Phosphoribosyl-ATP-Pyrophosphatase
3.6.1.34 H+-transportierende ATP-synthase
3.6.1.35 H+-transportierende ATPase
3.6.1.36 H+/K+-austauschende ATPase
3.6.1.38 Ca2+-transportierende ATPase
3.6.1.7 Acylphosphatase
4.1.1.11 Aspartat 1-decarboxylase
4.1.1.17 Ornithindecarboxylase
4.1.1.20 Diaminopimelatdecarboxylase
4.1.1.21 Phosphoribosylaminoimidazolcarboxylase
4.1.1.23 Orotidin-5'-phosphat-Decarboxylase
4.1.1.31 Phosphoenolpyruvatcarboxylase
4.1.1.32 Phosphoenolpyruvatcarboxykinase (GTP)
4.1.1.37 Uroporphyrinogendecarboxylase
4.1.1.41 Methylmalonyl-CoA-Decarboxylase
4.1.1.44 4-Carboxymuconolactondecarboxylase
4.1.1.48 Indol-3-glycerolphosphatsynthase
4.1.2.13 Fructosebisphosphataldolase
4.1.2.15 2-Dehydro-3-desoxyphosphoheptonataldolase
4.1.2.25 Dihydroneopterinaldolase
4.1.2.4 Desoxyribosephosphataldolase
4.1.3.1 Isocitratlyase
4.1.3.12 2-Isopropylmalatsynthase
4.1.3.18 Acetolactatsynthase
4.1.3.2 Malatsynthase
4.1.3.27 Anthranilatsynthase
4.1.3.36 Naphthoatsynthase
4.1.3.6 Citratlyase
4.1.3.7 Citrat(SI)synthase
4.1.99.3 Desoxyribodipyrimidinphotolyase
4.2.1.1 Carbonatdehydratase
4.2.1.10 3-Dehydrochinatdehydratase
4.2.1.11 Phosphopyruvathydratase
4.2.1.13 L-Serindehydratase
4.2.1.16 Threonindehydratase
4.2.1.17 Enoyl-CoA-Hydratase
4.2.1.19 Imidazol-Glycerolphosphatdehydratase
4.2.1.2 Fumarathydratase
4.2.1.20 Tryptophansynthase
4.2.1.24 Porphobilinogensynthase
4.2.1.3 Aconitathydratase
4.2.1.33 3-Isopropylmalatdehydratase
4.2.1.46 dTDP-Glucose-4,6-dehydratase
4.2.1.51 Prephenatdehydratase
4.2.1.52 Dihydrodipicolinatsynthase
4.2.1.70 Pseudouridylatsynthase
4.2.1.9 Dihydroxysäuredehydratase
4.2.1.96 Tetrahydrobiopterindehydratase
4.2.99.18 DNA-(apurinische oder apyrimidinische Sequenz)- lyase
4.2.99.2 Threoninsynthase
4.2.99.8 Cysteinsynthase
4.2.99.9 O-Succinylhomoserin-(Thiol)-lyase
4.3.1.1 Aspartat-Ammoniak-Lyase
4.3.1.12 Ornithincyclodeaminase
4.3.1.8 Hydroxymethylbilansynthase
4.3.2.1 Argininosuccinatlyase
4.3.2.2 Adenylsuccinatlyase
4.4.1.1 Cystathionin-γ-lyase
4.4.1.17 Holocytochrom c-Synthase
4.4.1.5 Lactoylglutathionlyase
4.4.1.8 Cystathionin-β-lyase
4.5.1.5 S-Carboxymethylcysteinsynthase
4.6.1.1 Adenylatcyclase
4.6.1.3 3-Dehydrochinatsynthase
4.6.1.4 Chorismatsynthase
4.99.1.1 Ferrochelatase
5.1.1.1 Alaninracemase
5.1.1.3 Glutamatracemase
5.1.1.7 Diaminopimaratepimerase
5.1.3.1 Ribulosephosphat-3-epimerase
5.1.3.2 UDP-Glucose-4-epimerase
5.2.1.8 Peptidylprolylisomerase
5.3.1.1 Triosephosphatisomerase
5.3.1.12 Glucuronatisomerase
5.3.1.16 N-(5'-Phospho-D-ribosylformimino)-5-amino-1-
5.3.1.24 Phosphoribosylanthranilatisomerase
5.3.1.6 Ribose-5-phosphatepimerase
5.3.1.8 Mannose-6-phosphatisomerase
5.3.1.9 Glucose-6-phosphatisomerase
5.3.3.2 Isopentenyldiphosphat-Δ-isomerase
5.4.2.1 Phosphoglyceratmutase
5.4.2.2 Phosphoglucomutase
5.4.2.8 Phosphomannomutase
5.4.2.9 Phosphoenolpyruvatmutase
5.4.3.8 Glutamat-1-semialdehyd-2,1-aminomutase
5.4.99.12 tRNA-PseudouridinsynthaseI
5.4.99.2 Methylmalonyl-CoA-Mutase
5.4.99.6 Isochorismatsynthase
5.5.1.2 3-Carboxy-cis,cis-muconat-cycloisomerase
5.99.1.2 DNA-Topoisomerase
5.99.1.3 DNA-Topoisomerase (ATP-hydrolysierend)
6.1.1.1 Tyrosin-tRNA-Ligase
6.1.1.10 Methionin-tRNA-Ligase
6.1.1.11 Serin-tRNA-Ligase
6.1.1.12 Aspartat-tRNA-Ligase
6.1.1.14 Glycin-tRNA-Ligase
6.1.1.15 Prolin-tRNA-Ligase
6.1.1.16 Cystein-tRNA-Ligase
6.1.1.17 Glutamat-tRNA-Ligase
6.1.1.19 Arginin-tRNA-Ligase
6.1.1.2 Tryptophan-tRNA-Ligase
6.1.1.20 Phenylalanin-tRNA-Ligase
6.1.1.4 Leucin-tRNA-Ligase
6.1.1.5 Isoleucin-tRNA-Ligase
6.1.1.6 Lysin-tRNA-Ligase
6.1.1.7 Alanin-tRNA-Ligase
6.1.1.9 Valin-tRNA-Ligase
6.2.1.26 O-Succinylbenzoat-CoA-Ligase
6.2.1.3 langkettige Fettsäure-CoA-Ligase
6.2.1.4 Succinat-CoA-Ligase (GTP-bildend)
6.2.1.5 Succinat-CoA-Ligase (ADP-bildend)
6.3.1.2 Glutamat-Ammoniak-Ligase
6.3.1.5 NAD+-Synthase
6.3.2.1 Pantoat-β-Alaninligase
6.3.2.12 Dihydrofolatsynthase
6.3.2.13 UDP-N-Acetylmuramoylalnyl-D-glutamat-2,6diaminopimelatligase
6.3.2.15 UDP-N-Acetylmuramoylalanyl-D-glutamyl-2,6-
6.3.2.17 Tetrahydrofolylpolyglutamatsynthase
6.3.2.4 D-Alanin-D-Alaninligase
6.3.2.6
Phosphoribosylaminoimidazolsuccinocarboxyamidsynthase
6.3.2.8 UDP-N-Acetylmuramat-Alaninligase
6.3.2.9 UDP-N-Acetylmuramoylalanin-D-Glutamatligase
6.3.3.1 Phosphoribosyl-Formylglycinamidincycloligase
6.3.3.3 Dethiobiotinsynthase
6.3.4.13 Phosphoribosylamin-Glycinligase
6.3.4.15 Biotin-[Acetyl-CoA-carboxylase]-ligase
6.3.4.2 CTP-Synthase
6.3.4.4 Adenylosuccinatsynthase
6.3.4.5 Argininosuccinatsynthase
6.3.4.6 Harnstoffcarboxylase
6.3.5.2 GMP-Synthase (Glutamin-hydrolysierend)
6.3.5.3 Phosphoribosylformylglycinamidinsynthase
6.3.5.5 Carbamoylphosphatsynthase (Glutamin-hydrolysierend)
6.4.1.1 Pyruvatcarboxylase
6.4.1.2 Acetyl-CoA-Carboxylase
6.4.1.3 Propionyl-CoA-Carboxylase
6.5.1.2 DNA-Ligase (NAD+). Enzyme aus Bacillus subtilis mit EC-Nummer 1.1.1 Alkoholdehydrogenase
1.1.1.100 3-Oxoacyl-[Acyl-Carrierprotein]-reductase
1.1.1.103 L-Threonin-3-dehydrogenase
1.1.1.125 2-Desoxy-D-gluconat-3-dehydrogenase
1.1.1.133 dTDP-4-Dehydrorhamnosereductase
1.1.1.14 L-Iditol-2-dehydrogenase
1.1.1.140 Sorbitol-6-phosphat-2-dehydrogenase
1.1.1.153 Sepiapterinreductase
1.1.1.169 2-Dehydropantoat-2-reductase
1.1.1.17 Mannitol-1-phosphat-5-dehydrogenase
1.1.1.18 Myoinositol-2-dehydrogenase
1.1.1.195 Cinnamylalkoholdehydrogenase
1.1.1.2 Alkoholdehydrogenase(NADP+)
1.1.1.202 1,3-Propandioldehydrogenase
1.1.1.204 Xanthindehydrogenase
1.1.1.205 IMP-Dehydrogenase
1.1.1.23 Histidinoldehydrogenase
1.1.1.236 Tropinonereductase
1.1.1.244 Methanoldehydrogenase
1.1.1.25 Shikimat-5-dehydrogenase
1.1.1.27 L-Lactatdehydrogenase
1.1.1.3 Homoserindehydrogenase
1.1.1.31 3-Hydroxyisobutyratdehydrogenase
1.1.1.35 3-Hydroxyacyl-CoA-Dehydrogenase
1.1.1.36 Acetoacetyl-CoA-Reductase
1.1.1.37 Malatdehydrogenase
1.1.1.38 Malatdehydrogenase (Oxaloacetat-decarboxylierend)
1.1.1.40 Malatdehydrogenase (Oxaloacetat- decarboxylierend)(NADP+)
1.1.1.42 Isocitratdehydrogenase (NADP+)
1.1.1.44 Phosphogluconatdehydrogenase (decarboxylierend)
1.1.1.47 Glucose-1-dehydrogenase
1.1.1.49 Glucose-6-phosphat-1-dehydrogenase
1.1.1.57 Fructuronatreductase
1.1.1.58 Tagaturonatreductase
1.1.1.69 Gluconat-5-dehydrogenase
1.1.1.8 Glycerol-3-phosphatdehydrogenase(NAD+)
1.1.1.83 D-Malatdehydrogenase (decarboxylierend)
1.1.1.85 3-Isopropylmalatdehydrogenase
1.1.1.86 Ketosäure-Reductoisomerase
1.1.1.93 Tartratdehydrogenase
1.1.1.95 Phosphoglyceratdehydrogenase
1.1.3.15 (S)-2-Hydroxysäureoxidase
1.1.3.22 Xanthinoxidase
1.1.99.5 Glycerol-3-phosphatdehydrogenase
1.1.99.8 Alkoholdehydrogenase (Akzeptor)
1.10.2.2 Ubichinol-Cytochrom c-Reductase
1.10.99.1 Plastochinol-Plastocyanin-Reductase
1.11.1.10 Chloridperoxidase
1.11.1.12 Phospholipid-Hydroperoxid Glutathionperoxidase
1.11.1.6 Katalase
1.11.1.9 Glutathionperoxidase
1.13.11.32 2-Nitropropandioxygenase
1.14.13.3 4-Hydroxyphenylacetat 3-monooxygenase
1.14.13.39 Stickoxidsynthase
1.14.14.1 unspezifische Monooxygenase
1.15.1.1 Superoxiddismutase
1.17.4.1 Ribonucleosiddiphosphatreductase
1.2.1.11 Aspartatsemialdehyddehydrogenase
1.2.1.12 Glyceraldehyd-3-phosphatdehydrogenase (phosphorylierend)
1.2.1.13 Glyceraldehyd-3-phosphatdehydrogenase (NADP+)(phosphorylierend)
1.2.1.16 Succinatsemialdehyddehydrogenase (NAD(P)+)
1.2.1.2 Formatdehydrogenase
1.2.1.27 Methylmalonatsemialdehyddehydrogenase (acylierend)
1.2.1.3 Aldehyddehydrogenase (NAD+)
1.2.1.38 N-Acetyl-γ-glutamylphosphatreductase
1.2.1.4 Aldehyddehydrogenase (NADP+)
1.2.1.41 Glutamat-5-semialdehyddehydrogenase
1.2.1.46 Formaldehyddehydrogenase
1.2.1.5 Aldehyddehydrogenase (NAD(P)+)
1.2.1.8 Betain-Aldehyddehydrogenase
1.2.2.2 Pyruvatdehydrogenase (Cytochrom)
1.2.3.1 Aldehydroxidase
1.2.3.3 Pyruvatoxidase
1.2.4.1 Pyruvatdehydrogenase (Lipoamid)
1.2.4.2 Oxoglutaratdehydrogenase (Lipoamid)
1.2.4.4 3-Methyl-2-oxobutanoatdehydrogenase (Lipoamid)
1.2.99.2 Kohlenmonoxiddehydrogenase
1.2.99.4 Formaldehyddismutase
1.3.1.12 Prephenatdehydrogenase
1.3.1.24 Biliverdinreductase
1.3.1.26 Dihydrodipicolinatreductase
1.3.1.28 2,3-Dihydro-2,3-dihydroxybenzoatdehydrogenase
1.3.1.34 2,4-Dienoyl-CoA-Reductase (NADPH)
1.3.1.35 Phosphatidylcholindesaturase
1.3.1.43 Cyclohexadienyldehydrogenase
1.3.1.9 Enoyl-[Acyl-Carrierprotein]-reductase (NADH)
1.3.3.1 Dihydroorotatoxidase
1.3.3.4 Protoporphyrinogenoxidase
1.3.3.5 Bilirubinoxidase
1.3.99.1 Succinatdehydrogenase
1.3.99.3 Acyl-CoA-Dehydrogenase
1.4.1.1 Alanindehydrogenase
1.4.1.13 Glutamatsynthase (NADPH)
1.4.1.14 Glutamatsynthase (NADH)
1.4.1.2 Glutamatdehydrogenase
1.4.1.20 Phenylalanindehydrogenase
1.4.1.3 Glutamatdehydrogenase (NAD(P)+)
1.4.1.8 Valindehydrogenase (NADP+)
1.4.1.9 Leucindehydrogenase
1.4.3.16 L-Aspartatoxidase
1.4.3.2 L-Aminosäureoxidase
1.4.4.2 Glycindehydrogenase (decarboxylierend)
1.4.7.1 Glutamatsynthase (Ferredoxin)
1.5.1.12 1-Pyrrolin-5-carboxylatdehydrogenase
1.5.1.2 Pyrrolin-5-carboxylatreductase
1.5.1.3 Dihydrofolatreductase
1.5.1.5 Methylentetrahydrofolatdehydrogenase (NADP+)
1.5.99.4 Nicotindehydrogenase
1.5.99.8 Prolindehydrogenase
1.6.2.4 NADPH-Ferrihämoproteinreductase
1.6.4.5 Thioredoxinreductase (NADPH)
1.6.5.3 NADH-Dehydrogenase (Ubichinon)
1.6.5.5 NADPH : Chinonreductase
1.6.6.4 Nitritreductase (NAD(P)H)
1.6.6.8 GMP-Reductase
1.6.8.2 NADPH-Dehydrogenase (Flavin)
1.6.99.2 NAD(P)H-Dehydrogenase (Chinon)
1.6.99.3 NADH-Dehydrogenase
1.7.3.3 Uratoxidase
1.7.99.4 Nitratreductase
1.7.99.7 Stickoxidreductase
1.8.1.2 Sulfitreductase (NADPH)
1.8.1.4 Dihydrolipoamiddehydrogenase
1.8.4.6 Protein-Methionin-S-oxidreductase
1.8.7.1 Sulfitreductase (Ferredoxin)
1.8.99.4
1.9.3.1 Cytochrom c-Oxidase
2.1.1.104 Caffeoyl-CoA O-Methyltransferase
2.1.1.107 Uroporphyrin-III c-Methyltransferase
2.1.1.14 5-Methyltetrahydropteroyltriglutamat-Homocystein
2.1.1.31 tRNA (Guanin-N1-)-Methyltransferase
2.1.1.37 DNA (Cytosin-5-)-Methyltransferase
2.1.1.45 Thymidylatsynthase
2.1.1.63 methylierte-DNA-Protein-Cystein-S- Methyltransferase
2.1.1.73 Sequenz-spezifische DNA-Methyltransferase (Cytosin-spezifisch)
2.1.2.1 Glycinhydroxymethyltransferase
2.1.2.10 Aminomethyltransferase
2.1.2.11 3-Methyl-2-oxobutanoathydroxymethyltransferase
2.1.2.2 Phosphoribosylglycinamid-Formyltransferase
2.1.2.3 Phosphoribosylaminoimidazolcarboxamid- Formyltransferase
2.1.2.9 Methionyl-tRNA-Formyltransferase
2.1.3.2 Aspartatcarbamoyltransferase
2.1.3.3 Ornithincarbamoyltransferase
2.2.1.1 Transketolase
2.2.1.2 Transaldolase
2.3.1.117 2,3,4,5-Tetrahydropyridin-2-carboxylat N- succinyltransferase
2.3.1.118 N-Hydroxyarylamin-O-Acetyltransferase
2.3.1.12 Dihydrolipoamid-S-Acetyltransferase
2.3.1.128 ribosomales Protein-Alanin-N-ACetyltransferase
2.3.1.16 Acetyl-CoA-C-Acyltransferase
2.3.1.18 Galactosid-O-Acetyltransferase
2.3.1.19 Phosphatbutyryltransferase
2.3.1.29 Glycin-C-Acetyltransferase
2.3.1.30 Serin-O-Acetyltransferase
2.3.1.35 Glutamat-N-Acetyltransferase
2.3.1.39 [Acyl-Carrierprotein]-S-Malonyltransferase
2.3.1.41 3-Oxoacyl-[Acyl-Carrierprotein]-synthase
2.3.1.46 Homoserin O-Succinyltransferase
2.3.1.47 8-Amino-7-oxononanoatsynthase
2.3.1.5 Arylamin-N-Acetyltransferase
2.3.1.60 Gentamicin-3'-N-Acetyltransferase
2.3.1.61 Dihydrolipoamid-S-Succinyltransferase
2.3.1.8 Phosphat-Acetyltransferase
2.3.1.81 Aminoglycosid N3'-Acetyltransferase
2.3.1.9 Acetyl-CoA-C-Acetyltransferase
2.3.2.13 Protein-Glutamin-γ-glutamyltransferase
2.3.2.2 γ-Glutamyltransferase
2.4.1.1 Phosphorylase
2.4.1.10 Levansucrase
2.4.1.17 Glucuronosyltransferase
2.4.1.18 1,4-α-Glucan verzweigendes Enzym
2.4.1.21 Stärkesynthase
2.4.1.44 Lipopolysaccharid-Galactosyltransferase
2.4.1.46 1,2-Diacylglycerol-3-β-galactosyltransferase
2.4.1.52 Poly(glycerolphosphat)-α-glucosyltransferase
2.4.1.56 Lipopolysaccharid-N-Acetylglucosaminyltransferase
2.4.2.1 Purin-Nucleosid-Phosphorylase
2.4.2.10 Orotat-Phosphoribosyltransferase
2.4.2.14 Amidophosphoribosyltransferase
2.4.2.17 ATP-Phosphoribosyltransferase
2.4.2.18 Anthranilat-Phosphoribosyltransferase
2.4.2.2 Pyrimidin-Nucleosid-Phosphorylase
2.4.2.29 Queuin-tRNA-Ribosyltransferase
2.4.2.4 Thymidinphosphorylase
2.4.2.7 Adeninphosphoribosyltransferase
2.4.2.8 Hypoxanthin-Phosphoribosyltransferase
2.4.2.9 Uracil-Phosphoribosyltransferase
2.5.1.10 Geranyltranstransferase
2.5.1.15 Dihydropteroatsynthase
2.5.1.16 Spermidinsynthase
2.5.1.18 Glutathiontransferase
2.5.1.19 3-Phosphoshikimat-1-carboxyvinyltransferase
5 2.5.1.29 Farnesyltranstransferase
2.5.1.3 Thiaminphosphatpyrophosphorylase
2.5.1.30 trans-Hexaprenyltranstransferase
2.5.1.31 Di-trans,poly-cis-decaprenylcistransferase
2.5.1.6 Methionin-Adenosyltransferase
2.5.1.7 UDP-N-Acetylglucosamin-1-carboxyvinyltransferase
2.5.1.8 tRNA-Isopentenyltransferase
2.5.1.9 Riboflavinsynthase
2.6.1.1 Aspartattransaminase
2.6.1.11 Acetylornithintransaminase
2.6.1.13 Ornithin-Oxosäure-Transaminase
2.6.1.16 Glutamin-Fructose-6-phosphattransaminase (isomerisierend)
2.6.1.19 4-Aminobutyrattransaminase 2.6.1.21 D-Alanintransaminase
2.6.1.44 Alanin-Glyoxylattransaminase
2.6.1.51 Serin-Pyruvattransaminase
2.6.1.52 Phosphoserintransaminase
2.6.1.62 Adenosylmethionin-8-amino-7- oxononanoattransaminase
2.6.1.9 Histidinolphosphattransaminase
2.7.1.11 6-Phosphofructokinase
2.7.1.113 Desoxyguanosinkinase
2.7.1.15 Ribokinase
2.7.1.16 Ribulokinase
2.7.1.17 Xylulokinase
2.7.1.21 Thymidinkinase
2.7.1.25 Adenylylsulfatkinase
2.7.1.30 Glycerolkinase
2.7.1.33 Pantothenatkinase
2.7.1.37 Proteinkinase
2.7.1.39 Homoserinkinase
2.7.1.4 Fructokinase
2.7.1.40 Pyruvatkinase
2.7.1.47 D-Ribulokinase
2.7.1.48 Uridinkinase
2.7.1.49 Hydroxymethylpyrimidinkinase
2.7.1.5 Rhamnulokinase
2.7.1.50 Hydroxyethylthiazolkinase
2.7.1.52 Fucokinase
2.7.1.56 1-Phosphofructokinase
2.7.1.6 Galactokinase
2.7.1.69 Protein-N(pai)-Phosphohistidin-Zucker- Phosphotransferase
2.7.1.71 Shikimatkinase
2.7.1.76 Desoxyadenosinkinase
2.7.2.1 Acetatkinase
2.7.2.11 Glutamat-5-kinase
2.7.2.3 Phosphoglyceratkinase
2.7.2.4 Aspartatkinase
2.7.2.7 Butyratkinase
2.7.2.8 Acetylglutamatkinase
2.7.3.3 Argininkinase
2.7.3.9 Phosphoenolpyruvat-Protein-Phosphotransferase
2.7.4.14 Cytidylatkinase
2.7.4.16 Thiaminphosphatkinase
2.7.4.3 Adenylatkinase
2.7.4.6 Nucleosiddiphosphatkinase
2.7.4.8 Guanylatkinase
2.7.6.1 Ribosephosphat-Pyrophosphokinase
2.7.6.3 2-Amino-4-hydroxy-6-hydroxymethyldihydropteridinpyro-
2.7.6.5 GTP-Pyrophosphokinase
2.7.7.10 UTP-Hexose-1-phosphat-Uridylyltransferase
2.7.7.19 Polynucleotid-Adenylyltransferase
2.7.7.24 Glucose-1-phosphat-Thymidylyltransferase
2.7.7.27 Glucose-1-phosphat-Adenylyltransferase
2.7.7.33 Glucose-1-phosphat-Cytidylyltransferase
2.7.7.39 Glycerol-3-phosphat-Cytidylyltransferase
2.7.7.4 Sulfat-Adenylyltransferase
2.7.7.41 Phosphatidat-Cytidylyltransferase
2.7.7.56 tRNA-Nucleotidyltransferase
2.7.7.6 DNA-bezogene RNA-Polymerase
2.7.7.7 DNA-bezogene DNA-Polymerase
2.7.7.8 Polyribonucleotid-Nucleotidyltransferase
2.7.7.9 UTP-Glucose-1-phosphat-Uridylyltransferase
2.7.8.12 CDP-Glycerol-Glycerophosphotransferase
2.7.8.13 Phospho-N-Acetylmuramoyl-Pentapeptidtransferase
2.7.8.5 CDP-Diacylglycerol-glycerol-3-phsophat-3- phosphatidyltransferase
2,7.8.7 Holo-[Acyl-Carrierprotein]-synthase
2.7.8.8 CDP-Diacylglycerol-Serin-O-phosphatidyltransferase
2.7.9.2 Pyruvat-Wasser-Dikinase
2.8.3.5 3-Oxosäure-CoA-Transferase
2.8.3.9 Butyrat-Acetoacetat-CoA-Transferase
3.1.1.1 Carboxylesterase
3.1.1.29 Aminoacyl-tRNA-Hydrolase
3.1.1.41 Cephalosporin-C-Deacetylase
3.1.1.61 Protein-Glutamat-Methylesterase
3.1.11.5 Exodesoxyribonuclease V
3.1.11.6 Exodesoxyribonuclease VII
3.1.2.2 Palmitoyl-CoA-Hydrolase
3.1.2.6 Hydroxyacylglutathionhydrolase
3.1.21.2 Desoxyribonuclease IV (Phage T4-induziert)
3.1.26.3 Ribonuclease III
3.1.26.4 Kälberthymus-Ribonuclease H
3.1.26.5 Ribonuclease P
3.1.3.1 alkalische Phosphatase
3.1.3.11 Fructosebisphosphatase
3.1.3.16 Phosphoproteinphosphatase
3.1.3.18 Phosphoglycolatphosphatase
3.1.3.25 Myoinositol-1(oder 4)-monophosphatase
3.1.3.48 Protein-Tyrosinphosphatase
3.1.3.5 5'-Nucleotidase
3.1.31.1 mikrococcische Nuclease
3.1.4.14 [Acyl-Carrierprotein]-Phosphodiesterase
3.1.4.16 2',3'-zyklisches Nucleotid 2'-Phosphodiesterase
3.1.4.46 Glycerophosphodiester-Phosphodiesterase
3.1.6.1 Arylsulfatase
3.2.1.1 α-Amylase
3.2.1.10 Oligo-1,6-glucosidase
3.2.1.135 Neopullulanase
3.2.1.14 Chitinase
3.2.1.20 α-Glucosidase
3.2.1.21 β-Glucosidase
3.2.1.22 α-Galactosidase
3.2.1.23 β-Galactosidase
3.2.1.25 β-Mannosidase
3.2.1.26 β-Fructofuranosidase
3.2.1.37 Xylan 1,4-β-xylosidase
3.2.1.4 Cellulase
3.2.1.41 α-Dextrin-endo-1,6-α-glucosidase
3.2.1.48 Sucrose-α-glucosidase
3.2.1.52 β-N-Acetylhexosaminidase
3.2.1.54 Cyclomaltodextrinase
3.2.1.55 α-N-Arabinofuranosidase
3.2.1.65 Levanase
3.2.1.7 Inulinase
3.2.1.73 Licheninase
3.2.1.74 Glucan-1,4-β-glucosidase
3.2.1.78 Mannan-endo-1,4-β-mannosidase
3.2.1.8 Endo-1,4-β-xylanase
3.2.1.86 6-Phospho-β-glucosidase
3.2.1.93 α,α-Phosphotrehalase
3.2.2.17 Desoxyribodipyrimidinndonucleosidase
3.2.2.20 DNA-3-Methyladeninglycosidase I
3.2.2.21 DNA-3-Methyladeninglycosidase II
3.2.2.23 Formamidopyrimidin-DNA-Glycosidase
3.3.2.1 Isochorismatase
3.3.2.3 Epoxidhydrolase
3.4.11.1 Leucyl-Aminopeptidase
3.4.11.18 Methionyl-Aminopeptidase
3.4.11.19 D-stereospezifische Aminopeptidase
3.4.11.5 Prolyl-Aminopeptidase
3.4.13.9 X-Pro-Dipeptidase
3.4.16.4 Serin-Typ D-Ala,D-Ala-Carboxypeptidase
3.4.17.8 Muramoylpentapeptid carboxypeptidase
3.4.19.1 Acylaminoacylpeptidase
3.4.19.3 PyroglutamylpeptidaseI
3.4.21.53 Endopeptidase Ia
3.4.21.62 Subtilisin
3.4.21.66 Thermitase
3.4.23.36 Signalpeptidase II
3.4.24.27 Thermolysin
3.4.24.28 Bacillolysin
3.4.24.57 O-Syaloglycoproteinendopeptidase
3.4.99.36 Leitpeptidase
3.4.99.41 mitochondriale Prozessierungs-Peptidase
3.5.1.1 Asparaginase
3.5.1.10 Formyltetrahydrofolatdeformylase
3.5.1.11 Penicillin-Amidase
3.5.1.16 Acetylornithindeacetylase
3.5.1.2 Glutaminase
3.5.1.24 Choloylglycinhydrolase
3.5.1.25 N-Acetylglucosamin-6-phosphatdeacetylase
3.5.1.27 N-Formylmethionylaminoacyl-tRNA-deformylase
3.5.1.28 N-Acetylmuramoyl-L-Alanin-Amidase
3.5.1.38 Glutamin-(Asparagin-)ase
3.5.1.5 Urease
3.5.2.3 Dihydroorotase
3.5.2.5 Allantoinase
3.5.2.6 β-Lactamase
3.5.3.1 Arginase
3.5.3.11 Agmatinase
3.5.4.10 IMP-Cyclohydrolase
3.5.4.12 dCMP-Deaminase
3.5.4.16 GTP-Cyclohydrolase I
3.5.4.19 Phosphoribosyl-AMP-Cyclohydrolase
3.5.4.2 Adenindeaminase
3.5.4.25 GTP-Cyclohydrolase II
3.5.4.5 Cytidindeaminase
3.5.4.9 Methenyltetrahydrofolatcyclohydrolase
3.6.1.23 dUTP-Pyrophosphatase
3.6.1.3 Adenosintriphosphatase
3.6.1.31 Phosphoribosyl-ATP-Pyrophosphatase
3.6.1.34 H+-transportierende ATP-Synthase
3.6.1.35 H+-transportierende ATPase
3.6.1.38 Ca2+-transportierende ATPase
3.6.1.41 bis(5'-Nucleosyl)-tetraphosphatase (symmetrisch)
3.6.1.45 UDP-Zuckerdiphosphatase
3.6.1.7 Acylphosphatase
3.7.1.9 2-Hydroxymuconatsemialdehydhydrolase
4.1.1.11 Aspartat-1-decarboxylase
4.1.1.17 Ornithindecarboxylase
4.1.1.18 Lysindecarboxylase
4.1.1.19 Arginindecarboxylase
4.1.1.20 Diaminopimelatdecarboxylase
4.1.1.21 Phosphoribosylaminoimidazolcarboxylase
4.1.1.23 Orotidin-5'-phosphatdecarboxylase
4.1.1.37 Uroporphyrinogendecarboxylase
4.1.1.39 Ribulosebisphosphatcarboxylase
4.1.1.41 Methylmalonyl-CoA-Decarboxylase
4.1.1.48 Indol-3-glycerolphosphatsynthase
4.1.1.49 Phosphoenolpyruvatcarboxykinase (ATP)
4.1.1.5 Acetolactatdecarboxylase
4.1.1.65 Phosphatidylserindecarboxylase
4.1.1.71 2-Oxoglutaratdecarboxylase
4.1.1.73 Tartratdecarboxylase
4.1.2.13 Fructosebisphosphataldolase
4.1.2.14 2-Dehydro-3-desoxyphosphogluconataldolase
4.1.2.15 2-Dehydro-3-desoxyphosphoheptonataldolase
4.1.2.25 Dihydroneopterinaldolase
4.1.2.4 Desoxyribosephosphataldolase
4.1.3.12 2-Isopropylmalatsynthase
4.1.3.16 4-Hydroxy-2-oxoglutarataldolase
4.1.3.18 Acetolactatsynthase
4.1.3.27 Anthranilatsynthase
4.1.3.36 Naphthoatsynthase
4.1.3.4 Hydroxymethylglutaryl-CoA-Lyase
4.1.3.7 Citrat(SI)synthase
4.2.1.10 3-Dehydrochinatdehydratase
4.2.1.11 Phosphopyruvathydratase
4.2.1.13 L-Serindehydratase
4.2.1.14 D-Serindehydratase
4.2.1.16 Threonindehydratase
4.2.1.17 Enoyl-CoA-Hydratase
4.2.1.19 Imidazolglycerolphosphatdehydratase
4.2.1.2 Fumarathydratase
4.2.1.20 Tryptophansynthase
4.2.1.24 Porphobilinogensynthase
4.2.1.3 Aconitathydratase
4.2.1.33 3-Isopropylmalatdehydratase
4.2.1.40 Glucaratdehydratase
4.2.1.41 5-Dehydro-4-desoxyglucaratdehydratase
4.2.1.46 dTDP-Glucose-4,6-dehydratase
4.2.1.49 Urocanathydratase
4.2.1.51 Prephenatdehydratase
4.2.1.52 Dihydrodipicolinatsynthase
4.2.1.7 Altronatdehydratase
4.2.1.75 Uroporphyrinogen-III-Synthase
4.2.1.8 Mannonatdehydratase
4.2.1.9 Dihydroxysäuredehydratase
4.2.2.10 Pectinlyase
4.2.2.2 Pectatlyase
4.2.99.11 Methylglyoxalsynthase
4.2.99.18 DNA-(apurinische oder apyrimidinische Sequenz)- lyase
4.2.99.2 Threoninsynthase
4.2.99.8 Cysteinsynthase
4.3.1.1 Aspartat-Ammoniak-Lyase
4.3.1.3 Histidin-Ammoniak-Lyase
4.3.1.8 Hydroxymethylbilansynthase
4.3.2.1 Argininosuccinatlyase
4.3.2.2 Adenylsuccinatlyase
4.4.1.17 Holocytochrom c-Synthase
4.4.1.5 Lactoylglutathionlyase
4.4.1.8 Cystathionin-β-lyase
4.6.1.3 3-Dehydrochinatsynthase
4.6.1.4 Chorismatsynthase
4.99.1.1 Ferrochelatase
5.1.1.1 Alaninracemase
5.1.1.13 Aspartatracemase
5.1.1.3 Glutamatracemase
5.1.1.7 Diaminopimaratepimerase
5.1.2.2 Mandelatracemase
5.1.3.1 Ribulosephosphat-3-epimerase
5.1.3.2 UDP-Glucose-4-epimerase
5.1.3.4 L-Ribulosephosphat-4-epimerase
5.2.1.8 Peptidylprolylisomerase
5.3.1.1 Triosephosphatisomerase
5.3.1.10 Glucosamin-6-phosphatisomerase
5.3.1.12 Glucuronatisomerase
5.3.1.14 L-Rhamnoseisomerase
5.3.1.16 N-(5'-Phospho-D-ribosylformimino)-5-amino-1-
5.3.1.17 4-Desoxy-L-threo-5-hexosulose-uronatketoisomerase
5.3.1.24 Phosphoribosylanthranilatisomerase
5.3.1.4 L-Arabinoseisomerase
5.3.1.5 Xyloseisomerase
5.3.1.6 Ribose-5-phosphatepimerase
5.3.1.8 Mannose-6-phosphatisomerase
5.3.1.9 Glucose-6-phosphatisomerase
5.4.2.1 Phosphoglyceratmutase
5.4.2.6 β-Phosphoglucomutase
5.4.2.7 Phosphopentomutase
5.4.3.8 Glutamat-1-semialdehyd-2,1-aminomutase
5.4.99.12 tRNA-Pseudouridinsynthase I
5.4.99.5 Chorismatmutase
5.4.99.6 Isochorismatsynthase
5.4.99.7 Lanosterolsynthase
5.5.1.1 Muconatcycloisomerase
5.5.1.7 Chloromuconatcycloisomerase
5.99.1.2 DNA-Topoisomerase
5.99.1.3 DNA-Topoisomerase (ATP-hydrolysierend)
6.1.1.1 Tyrosin-tRNA-Ligase
6.1.1.10 Methionin-tRNA-Ligase
6.1.1.11 Serin-tRNA-Ligase
6.1.1.12 Aspartat-tRNA-Ligase
6.1.1.14 Glycin-tRNA-Ligase
6.1.1.15 Prolin-tRNA-Ligase
6.1.1.16 Cystein-tRNA-Ligase
6.1.1.17 Glutamat-tRNA-Ligase
6.1.1.19 Arginin-tRNA-Ligase
6.1.1.2 Tryptophan-tRNA-Ligase
6.1.1.20 Phenylalanin-tRNA-Ligase
6.1.1.21 Histidin-tRNA-Ligase
6.1.1.22 Asparagin-tRNA-Ligase
6.1.1.3 Threonin-tRNA-Ligase
6.1.1.4 Leucin-tRNA-Ligase
6.1.1.5 Isoleucin-tRNA-Ligase
6.1.1.6 Lysin-tRNA-Ligase
6.1.1.7 Alanin-tRNA-Ligase
6.1.1.9 Valin-tRNA-Ligase
6.2.1.1 Acetat-CoA-Ligase
6.2.1.14 6-Carboxyhexanoat-CoA-Ligase
6.2.1.26 O-Succinylbenzoat-CoA-Ligase
6.2.1.3 langkettige Fettsäure-CoA-Ligase
6.2.1.4 Succinat-CoA-Ligase (GTP-bildend)
6.2.1.5 Succinat-CoA-Ligase (ADP-bildend)
6.3.1.2 Glutamat-Ammoniak-Ligase
6.3.1.5 NAD+-Synthase
6.3.2.1 Pantoat-β-Alaninligase
6.3.2.13 UDP-N-Acetylmuramoylalny1-D-Glutamat-2,6- diaminopimelatligase
6.3.2.15 UDP-N-Acetylmuramoylalanyl-D-Glutamyl-2,6-
6.3.2.17 Tetrahydrofolylpolyglutamatsynthase
6.3.2.4 D-Alanin-D-Alaninligase
6.3.2.6 Phosphoribosylaminoimidazolsuccinocarboxyamidsynthase
6.3.2.8 UDP-N-Acetylmuramat-Alaninligase
6.3.2.9 UDP-N-Acetylmuramoylalanin-D-Glutamatligase
6.3.3.1 Phosphoribosyl-Formylglycinamidincycloligase
6.3.3.2 5-Formyltetrahydrofolatcycloligase
6.3.3.3 Dethiobiotinsynthase
6.3.4.13 Phosphoribosylamin-Glycinligase
6.3.4.14 Biotincarboxylase
6.3.4.2 CTP-Synthase
6.3.4.4 Adenylosuccinatsynthase
6.3.4.5 Argininosuccinatsynthase
6.3.5.1 NAD+-Synthase (Glutamin-hydrolysierend)
6.3.5.2 GMP-Synthase (Glutamin-hydrolysierend)
6.3.5.3 Phosphoribosylformylglycinamidinsynthase
6.3.5.4 Asparaginsynthase (Glutamin-hydrolysierend)
6.3.5.5 Carbamoylphosphatsynthase (Glutamin-hydrolysierend)
6.4.1.1 Pyruvatcarboxylase
6.4.1.2 Acetyl-CoA-Carboxylase
6.5.1.2 DNA-Ligase (NAD+). Enzyme aus Lactococcus lactis mit EC Nummer 1.1.1.1 Alkoholdehydrogenase
1.1.1.100 3-Oxoacyl-[Acyl-Carrierprotein]-reductase
1.1.1.14 L-Iditol-2-dehydrogenase
1.1.1.169 2-Dehydropantoat-2-reductase
1.1.1.17 Mannitol-1-phosphat-5-dehydrogenase
1.1.1.184 Carbonylreductase (NADPH)
1.1.1.195 Cinnamylalkoholdehydrogenase
1.1.1.205 IMP-Dehydrogenase
1.1.1.218 Morphin-6-dehydrogenase
1.1.1.219 Dihydrokaempferol-4-reductase
1.1.1.23 Histidinoldehydrogenase
1.1.1.25 Shikimat-5-dehydrogenase
1.1.1.27 L-Lactatdehydrogenase
1.1.1.3 Homoserindehydrogenase
1.1.1.31 3-Hydroxyisobutyratdehydrogenase
1.1.1.38 Malatdehydrogenase (Oxaloacetat-decarboxylierend)
1.1.1.4 (R,R)-Butanedioldehydrogenase
1.1.1.40 Malatdehydrogenase (Oxaloacetatdecarboxylierend)(NADP+)
1.1.1.42 Isocitratdehydrogenase (NADP+)
1.1.1.44 Phosphogluconatdehydrogenase (decarboxylierend)
1.1.1.49 Glucose-6-phosphat-1-dehydrogenase
1.1.1.53 3-α(oder 20-β-Hydroxysteroiddehydrogenase
1.1.1.57 Fructuronatreductase
1.1.1.6 Glyceroldehydrogenase
1.1.1.8 Glycerol-3-phosphatdehydrogenase(NAD+)
1.1.1.85 3-Isopropylmalatdehydrogenase
1.1.1.86 Ketosäure-Reductoisomerase
1.1.1.88 Hydroxymethylglutaryl-CoA-Reductase
1.1.1.95 Phosphoglyceratdehydrogenase
1.1.3.15 (S)-2-Hydroxysäureoxidase
1.1.99.5 Glycerol-3-phosphatdehydrogenase
1.11.1.1 NADH-Peroxidase
1.11.1.9 Glutathionperoxidase
1.12.2.1 Cytochrom c3-Hydrogenase
1.15.1.1 Superoxiddismutase
1.16.1.1 Quecksilber(II)-Reductase
1.17.4.1 Ribonucleosiddiphosphatreductase
1.17.4.2 Ribonucleosidtriphosphatreductase
1.2.1.10 Acetaldehyddehydrogenase (acetylierend)
1.2.1.11 Aspartatsemialdehyddehydrogenase
1.2.1.12 Glyceraldehyd-3-phosphatdehydrogenase (phosphorylierend)
1.2.1.2 Formatdehydrogenase
1.2.1.3 Aldehyddehydrogenase (NAD+)
1.2.1.38 N-Acetyl-γ-glutamylphosphatreductase
1.2.1.41 Glutamat-5-Semialdehyddehydrogenase
1.2.2.2 Pyruvatdehydrogenase (Cytochrom)
1.2.3.3 Pyruvatoxidase
1.2.4.1 Pyruvatdehydrogenase (Lipoamid)
1.2.4.4 3-Methyl-2-oxobutanoatdehydrogenase (Lipoamid)
1.3.1.12 Prephenatdehydrogenase
1.3.1.26 Dihydrodipicolinatreductase
1.3.1.9 Enoyl-[Acyl-Carrierprotein]-reductase (NADH)
1.3.3.1 Dihydroorotatoxidase
1.3.99.1 Succinatdehydrogenase
1.4.1.1 Alanindehydrogenase
1.4.1.13 Glutamatsynthase (NADPH)
1.4.1.14 Glutamatsynthase (NADH)
1.4.7.1 Glutamatsynthase (Ferredoxin)
1.5.1.2 Pyrrolin-5-carboxylatreductase
1.5.1.24 N5-(Carboxyethyl)ornithinsynthase
1.5.1.3 Dihydrofolatreductase
1.5.1.5 Methylentetrahydrofolatdehydrogenase (NADP+)
1.6.4.2 Glutathionreductase (NADPH)
1.6.4.5 Thioredoxinreductase (NADPH)
1.6.5.3 NADH-Dehydrogenase (Ubichinon)
1.6.6.8 GMP-Reductase
1.7.99.5 5,10-Methylentetrahydrofolatreductase (FADH2)
1.8.1.4 Dihydrolipoamiddehydrogenase
1.97.1.4 [Pyruvatformatlyase]-aktivierendes Enzym
2.1.1.104 Caffeoyl-CoA-O-Methyltransferase
2.1.1.14 5-Methyltetrahydropteroyltriglutamat-Homocystein
2.1.1.31 tRNA-(Guanin-N1-)-methyltransferase
2.1.1.45 Thymidylatsynthase
2.1.1.63 methylierte-DNA-Protein-Cystein-S- Methyltransferase
2.1.2.1 Glycinhydroxymethyltransferase
2.1.2.2 Phosphoribosylglycinamid-Formyltransferase
2.1.2.3 Phosphoribosylaminoimidazolcarboxamid- Formyltransferase
2.1.2.9 Methionyl-tRNA-Formyltransferase
2.1.3.2 Aspartatcarbamoyltransferase
2.1.3.3 Ornithincarbamoyltransferase
2.2.1.1 Transketolase
2.3.1.12 Dihydrolipoamid S-Acetyltransferase
2.3.1.128 ribosomales Protein-Alanin-N-Acetyltransferase
2.3.1.18 Galactosid-O-Acetyltransferase
2.3.1.30 Serin-O-Acetyltransferase
2.3.1.35 Glutamat-N-Acetyltransferase
2.3.1.39 [Acyl-Carrierprotein]-S-Malonyltransferase
2.3.1.41 3-Oxoacyl-[Acyl-Carrierprotein]-Synthase
2.3.1.46 Homoserin-O-Succinyltransferase
2.3.1.54 Format c-Acetyltransferase
2.3.1.57 Diamin-N-Acetyltransferase
2.3.1.8 Phosphat-Acetyltransferase
2.3.1.9 Acetyl-CoA-C-Acetyltransferase
2.4.1.1 Phosphorylase
2.4.1.21 Stärkesynthase
2.4.1.24 1,4-α-Glucan-6-α-glucosyltransferase
2.4.1.25 4-α-Glucanotransferase
2.4.1.52 Poly(glycerolphosphat)-α-glucosyltransferase
2.4.2.1 Purin-Nucleosid-Phosphorylase
2.4.2.10 Orotat-Phosphoribosyltransferase
2.4.2.14 Amidophosphoribosyltransferase
2.4.2.17 ATP-Phosphoribosyltransferase
2.4.2.18 Anthranilat-Phosphoribosyltransferase
2.4.2.2 Pyrimidin-Nucleosid-Phosphorylase
2.4.2.29 Queuin-tRNA-Ribosyltransferase
2.4.2.3 Uridinphosphorylase
2.4.2.4 Thymidinphosphorylase
2.4.2.7 Adenin-Phosphoribosyltransferase
2.4.2.8 Hypoxanthin-Phosphoribosyltransferase
2.4.2.9 Uracil-Phosphoribosyltransferase
2.5.1.10 Geranyltranstransferase
2.5.1.15 Dihydropteroatsynthase
2.5.1.19 3-Phosphoshikimat-1-carboxyvinyltransferase
2.5.1.29 Farnesyltranstransferase
2.5.1.3 Thiaminphosphatpyrophosphorylase
2.5.1.31 Di-trans,poly-cis-decaprenylcistransferase
2.5.1.6 Methionin-Adenosyltransferase
2.5.1.7 UDP-N-Acetylglucosamin-1-carboxyvinyltransferase
2.5.1.8 tRNA-Isopentenyltransferase
2.5.1.9 Riboflavinsynthase
2.6.1.1 Aspartattransaminase
2.6.1.16 Glutamin-Fructose-6-phosphattransaminase (isomerisierend)
2.6.1.45 Serin-Glyoxylattransaminase
2.6.1.52 Phosphoserintransaminase
2.6.1.9 Histidinolphosphattransaminase
2.7.1.107 Diacylglycerolkinase
2.7.1.11 6-Phosphofructokinase
2.7.1.113 Desoxyguanosinkinase
2.7.1.15 Ribokinase
2.7.1.17 Xylulokinase
2.7.1.21 Thymidinkinase
2.7.1.30 Glycerolkinase
2.7.1.33 Pantothenatkinase
2.7.1.36 Mevalonatkinase
2.7.1.39 Homoserinkinase
2.7.1.4 Fructokinase
2.7.1.40 Pyruvatkinase
2.7.1.48 Uridinkinase
2.7.1.49 Hydroxymethylpyrimidinkinase
2.7.1.50 Hydroxyethylthiazolkinase
2.7.1.56 1-Phosphofructokinase
2.7.1.6 Galactokinase
2.7.1.69 Protein-N(pai)-Phosphohistidin-Zucker- Phosphotransferase
2.7.1.71 Shikimatkinase
2.7.1.76 Desoxyadenosinkinase
2.7.1.95 Kanamycinkinase
2.7.2.1 Acetatkinase
2.7.2.11 Glutamat-5-kinase
2.7.2.2 Carbamatkinase
2.7.2.3 Phosphoglyceratkinase
2.7.2.4 Aspartatkinase
2.7.2.8 Acetylglutamatkinase
2.7.3.9 Phosphoenolpyruvat-Protein-Phosphotransferase
2.7.4.14 Cytidylatkinase
2.7.4.3 Adenylatkinase
2.7.4.8 Guanylatkinase
2.7.6.1 Ribosephosphat-Pyrophosphokinase
2.7.6.5 GTP-Pyrophosphokinase
2.7.7.10 UTP-Hexose-1-phosphat-Uridylyltransferase
2.7.7.24 Glucose-1-phosphat-Thymidylyltransferase
2.7.7.27 Glucose-1-phosphat-Adenylyltransferase
2.7.7.39 Glycerol-3-phosphat-Cytidylyltransferase
2.7.7.41 Phosphatidat-Cytidylyltransferase
2.7.7.6 DNA-bezogene RNA-Polymerase
2.7.7.7 DNA-bezogene DNA-Polymerase
2.7.7.8 Polyribonucleotid-Nucleotidyltransferase
2.7.7.9 UTP-Glucose-1-phosphat-Uridylyltransferase
2.7.8.12 CDP-Glycerol-Glycerophosphotransferase
2.7.8.13 Phospho-N-Acetylmuramoylpentapeptidtransferase
2.7.8.5 CDP-Diacylglycerol-glycerol-3-phsophat 3- phosphatidyltransferase
2.7.8.7 Holo-[Acyl-Carrierprotein]-synthase
3.1.1.29 Aminoacyl-tRNA-Hydrolase
3.1.11.5 Exodesoxyribonuclease V
3.1.11.6 Exodesoxyribonuclease VII
3.1.21.3 TypI Sequenz-spezifische Desoxyribonuclease
3.1.26.3 Ribonuclease III
3.1.26.4 Kälberthymus-Ribonuclease H
3.1.26.5 Ribonuclease P
3.1.3.11 Fructosebisphosphatase
3.1.3.15 Histidinolphosphatase
3.1.3.16 Phosphoproteinphosphatase
3.1.3.25 Myoinositol-1(oder 4)-monophosphatase
3.1.3.3 Phosphoserinphosphatase
3.1.3.48 Protein-Tyrosinphosphatase
3.1.3.5 5'-Nucleotidase
3.1.4.14 [Acyl-Carrierprotein]-Phosphodiesterase
3.1.4.16 2',3'-zyclisches Nucleotid-2'-Phosphodiesterase
3.1.4.46 Glycerophosphodiester-Phosphodiesterase
3.1.6.1 Arylsulfatase
3.1.7.2 Guanosin-3',5'-bis(diphosphat)-3'-pyrophosphatase
3.2.1.1 α-Amylase
3.2.1.10 Oligo-1,6-glucosidase
3.2.1.135 Neopullulanase
3.2.1.14 Chitinase
3.2.1.20 α-Glucosidase
3.2.1.21 β-Glucosidase
3.2.1.23 β-Galactosidase
3.2.1.28 α,α-Trehalase
3.2.1.37 Xylan-1,4-β-xylosidase
3.2.1.41 α-Dextrin-endo-1,6-α-glucosidase
3.2.1.52 β-N-Acetylhexosaminidase
3.2.1.54 Cyclomaltodextrinase
3.2.1.70 Glucan-1,6-α-glucosidase
3.2.1.8 Endo-1,4-β-xylanase
3.2.1.86 6-Phospho-β-glucosidase
3.2.1.93 α,α-Phosphotrehalase
3.2.1.96 Mannosylglycoprotein-endo-β-N-
Acetylglucosaminidase
3.2.2.17 Desoxyribodipyrimidinndonucleosidase
3.2.2.23 Formamidopyrimidin-DNA-Glycosidase
3.4.11.15 Lysyl-Aminopeptidase
3.4.11.18 Methionyl-Aminopeptidase
3.4.11.2 Membrane-Alanyl-Aminopeptidase
3.4.11.5 Prolyl-Aminopeptidase
3.4.13.18 Cytosol unspezifische Dipeptidase
3.4.13.9 X-Pro-Dipeptidase
3.4.14.11 XAA-Pro-Dipeptidylpeptidase
3.4.16.4 Serin-Typ D-Ala,D-Ala-Carboxypeptidase
3.4.23.36 Signalpeptidase II
3.4.24.57 O-Syaloglycoproteinendopeptidase
3.4.99.36 Leitpeptidase
3.4.99.41 mitochondriale Prozessierungs-Peptidase
3.5.1.1 Asparaginase
3.5.1.11 Penicillin-Amidase
3.5.1.16 Acetylornithindeacetylase
3.5.1.18 Succinyldiaminopimelatdesuccinylase
3.5.1.24 Choloylglycinhydrolase
3.5.1.25 N-Acetylglucosamin-6-phosphatdeacetylase
3.5.2.12 6-Aminohexanoat-zyklisches Dimer-Hydrolase
3.5.2.3 Dihydroorotase
3.5.3.6 Arginindeiminase
3.5.4.10 IMP-Cyclohydrolase
3.5.4.12 dCMP-Deaminase
3.5.4.16 GTP-Cyclohydrolase I
3.5.4.19 Phosphoribosyl-AMP-Cyclohydrolase
3.5.4.25 GTP-Cyclohydrolase II
3.5.4.4 Adenosindeaminase
3.5.4.5 Cytidindeaminase
3.5.4.9 Methenyltetrahydrofolatcyclohydrolase
3.6.1.17 bis(5'-Nucleosyl)-tetraphosphatase (asymmetrisch)
3.6.1.23 dUTP-Pyrophosphatase
3.6.1.3 Adenosintriphosphatase
3.6.1.31 Phosphoribosyl-ATP-Pyrophosphatase
3.6.1.34 H+-transportierende ATP-Synthase
3.6.1.35 H+-transportierende ATPase
3.6.1.36 H+/K+ austauschende ATPase
3.6.1.38 Ca2+-transportierende ATPase
4.1.1.1 Pyruvatdecarboxylase
4.1.1.15 Glutamatdecarboxylase
4.1.1.20 Diaminopimelatdecarboxylase
4.1.1.21 Phosphoribosylaminoimidazolcarboxylase
4.1.1.23 Orotidin-5'-phosphatdecarboxylase
4.1.1.33 Diphosphomevalonatdecarboxylase
4.1.1.44 4-Carboxymuconolactonedecarboxylase
4.1.1.48 Indol-3-glycerolphosphatsynthase
4.1.1.5 Acetolactatdecarboxylase
4.1.1.71 2-Oxoglutaratdecarboxylase
4.1.2.13 Fructosebisphosphataldolase
4.1.2.15 2-Dehydro-3-desoxyphosphoheptonataldolase
4.1.2.25 Dihydroneopterinaldolase
4.1.2.4 Desoxyribosephosphataldolase
4.1.3.18 Acetolactatsynthase
4.1.3.27 Anthranilatsynthase
4.1.3.36 Naphthoatsynthase
4.1.3.5 Hydroxymethylglutaryl-CoA-Synthase
4.1.3.6 Citratlyase
4.1.3.7 Citrat(SI)synthase
4.2.1.10 3-Dehydrochinatdehydratase
4.2.1.11 Phosphopyruvathydratase
4.2.1.12 Phosphogluconatdehydratase
4.2.1.13 L-Serindehydratase
4.2.1.16 Threonindehydratase
4.2.1.19 Imidazolglycerolphosphatdehydratase
4.2.1.20 Tryptophansynthase
4.2.1.3 Aconitathydratase
4.2.1.33 3-Isopropylmalatdehydratase
4.2.1.51 Prephenatdehydratase
4.2.1.52 Dihydrodipicolinatsynthase
4.2.1.8 Mannonatdehydratase
4.2.1.9 Dihydroxysäuredehydratase
4.2.99.18 DNA-(apurinische oder apyrimidinische Sequenz)- lyase
4.2.99.2 Threoninsynthase
4.2.99.8 Cysteinsynthase
4.2.99.9 O-Succinylhomoserin-(Thiol)-lyase
4.3.2.1 Argininosuccinatlyase
4.3.2.2 Adenylsuccinatlyase
4.6.1.3 3-Dehydrochinatsynthase
4.6.1.4 Chorismatsynthase
4.99.1.1 Ferrochelatase
5.1.1.1 Alaninracemase
5.1.1.13 Aspartatracemase
5.1.1.3 Glutamatracemase
5.1.3.1 Ribulosephosphat-3-epimerase
5.1.3.2 UDP-Glucose-4-epimerase
5.1.3.3 Aldose-1-epimerase
5.2.1.8 Peptidylprolylisomerase
5.3.1.1 Triosephosphatisomerase
5.3.1.10 Glucosamin-6-phosphatisomerase
5.3.1.12 Glucuronatisomerase
5.3.1.16 N-(5'-Phospho-D-ribosylformimino)-5-amino-1-
5.3.1.24 Phosphoribosylanthranilatisomerase
5.3.1.4 L-Arabinoseisomerase
5.3.1.5 Xyloseisomerase
5.3.1.6 Ribose-5-phosphatepimerase
5.3.1.8 Mannose-6-phosphatisomerase
5.3.1.9 Glucose-6-phosphatisomerase
5.4.2.1 Phosphoglyceratmutase
5.4.2.6 β-Phosphoglucomutase
5.4.2.7 Phosphopentomutase
5.4.2.8 Phosphomannomutase
5.4.99.12 tRNA-PseudouridinsynthaseI
5.4.99.5 Chorismatmutase
5.4.99.6 Isochorismatsynthase
5.5.1.7 Chloromuconatcycloisomerase
5.99.1.3 DNA-Topoisomerase (ATP-hydrolysierend)
6.1.1.1 Tyrosin-tRNA-Ligase
6.1.1.10 Methionin-tRNA-Ligase
6.1.1.11 Serin-tRNA-Ligase
6.1.1.12 Aspartat-tRNA-Ligase
6.1.1.14 Glycin-tRNA-Ligase
6.1.1.15 Prolin-tRNA-Ligase
6.1.1.16 Cystein-tRNA-Ligase
6.1.1.17 Glutamat-tRNA-Ligase
6.1.1.19 Arginin-tRNA-Ligase
6.1.1.2 Tryptophan-tRNA-Ligase
6.1.1.20 Phenylalanin-tRNA-Ligase
6.1.1.21 Histidin-tRNA-Ligase
6.1.1.22 Asparagin-tRNA-Ligase
6.1.1.3 Threonin-tRNA-Ligase
6.1.1.4 Leucin-tRNA-Ligase
6.1.1.5 Isoleucin-tPNA-Ligase
6.1.1.6 Lysin-tRNA-Ligase
6.1.1.7 Alanin-tRKA-Ligase
6.1.1.9 Valin-tRNA-Ligase
6.2.1.1 Acetat-CoA-Ligase
6.2.1.22 Citrat(Pro-3S)-lyaseligase
6.2.1.26 O-Succinylbenzoat-CoA-Ligase
6.2.1.3 langkettige Fettsäure-CoA-Ligase
6.3.1.1 Aspartat-Ammoniakligase
6.3.1.2 Glutamat-Ammoniakligase
6.3.1.5 NAD+-Synthase
6.3.2.13 UDP-N-Acetylmuramoylalnyl-D-glutamat-2,6- diaminopimelatligase
6.3.2.15 UDP-N-Acetylmuramoylalanyl-D-glutamyl-2,6-
6.3.2.17 Tetrahydrofolylpolyglutamatsynthase
6.3.2.4 D-Alanin-D-Alaninligase
6.3.2.6 Phosphoribosylaminoimidazolsuccinocarboxyamidsynthase
6.3.2.8 UDP-N-Acetylmuramat-Alaninligase
6.3.2.9 UDP-N-Acetylmuramoylalanin-D-Glutamatligase
6.3.3.1 Phosphoribosyl-Formylglycinamidincycloligase
6.3.4.13 Phosphoribosylamin-Glycinligase
6.3.4.14 Biotincarboxylase
6.3.4.15 Biotin-[Acetyl-CoA-carboxylase]-ligase
6.3.4.2 CTP-Synthase
6.3.4.3 Format-Tetrahydrofolatligase
6.3.4.4 Adenylosuccinatsynthase
6.3.4.5 Argininosuccinatsynthase
6.3.5.1 NAD+-Synthase (Glutamin-hydrolysierend)
6.3.5.2 GMP-Synthase (Glutamin-hydrolysierend)
6.3.5.3 Phosphoribosylformylglycinamidinsynthase
6.3.5.4 Asparaginsynthase (Glutamin-hydrolysierend)
6.3.5.5 Carbamoylphosphatsynthase (Glutamin-hydrolysierend)
6.4.1.1 Pyruvatcarboxylase
6.4.1.2 Acetyl-CoA-Carboxylase
6.5.1.2 DNA-Ligase (NAD+). Enzyme aus Clostridium acetobutylicum mit EC-Nummer 1.1.1.1 Alkoholdehydrogenase
1.1.1.100 3-Oxoacyl-[Acyl-Carrierprotein]-reductase
1.1.1.125 2-Desoxy-D-gluconat-3-dehydrogenase
1.1.1.169 2-Dehydropantoat-2-reductase
1.1.1.17 Mannitol-1-phosphat-5-dehydrogenase
1.1.1.195 Cinnamyl-Alkoholdehydrogenase
1.1.1.202 1,3-Propandioldehydrogenase
1.1.1.205 IMP-Dehydrogenase
1.1.1.211 lange Ketten-Hydroxyacyl-CoA-Dehydrogenase
1.1.1.219 Dihydrokaempferol-4-reductase
1.1.1.23 Histidinoldehydrogenase
1.1.1.25 Shikimat-5-dehydrogenase
1.1.1.27 L-Lactatdehydrogenase
1.1.1.28 D-Lactatdehydrogenase
1.1.1.29 Glyceratdehydrogenase
1.1.1.3 Homoserindehydrogenase
1.1.1.31 3-Hydroxyisobutyratdehydrogenase
1.1.1.35 3-Hydroxyacyl-CoA-Dehydrogenase
1.1.1.37 Malatdehydrogenase
1.1.1.38 Malatdehydrogenase (Oxaloacetat-decarboxylierend)
1.1.1.40 Malatdehydrogenase (Oxaloacetat- decarboxylierend)(NADP+)
1.1.1.41 Isocitratdehydrogenase (NAD+)
1.1.1.47 Glucose-1-dehydrogenase
1.1.1.57 Fructuronatreductase
1.1.1.58 Tagaturonatreductase
1.1.1.69 Gluconat-5-dehydrogenase
1.1.1.8 Glycerol-3-phosphatdehydrogenase (NAD+)
1.1.1.85 3-Isopropylmalatdehydrogenase
1.1.1.86 Ketosäure-Reductoisomerase
1.1.1.95 Phosphoglyceratdehydrogenase
1.1.99.6 D-2-Hydroxysäuredehydrogenase
1.1.99.8 Alkoholdehydrogenase (Akzeptor)
1.10.2.2 Ubichinol-Cytochrom c-Reductase
1.11.1.10 Chloridperoxidase
1.11.1.12 Phospholipid-Hydroperoxid-Glutathionperoxidase
1.11.1.9 Glutathionperoxidase
1.12.1.2 Hydrogendehydrogenase
1.12.2.1 Cytochrom c3-Hydrogenase
1.12.99.1 Coenzym f420-Hydrogenase
1.15.1.1 Superoxiddismutase
1.17.4.1 Ribonucleosiddiphosphatreductase
1.17.4.2 Ribonucleosidtriphosphatreductase
1.18.1.1 Rubredoxin-NAD+-Reductase
1.18.6.1 Nitrogenase
1.18.99.1 Hydrogenase
1.2.1.10 Acetaldehyddehydrogenase (acetylierend)
1.2.1.11 Aspartatsemialdehyddehydrogenase
1.2.1.12 Glyceraldehyd-3-phosphatdehydrogenase (phosphorylierend)
1.2.1.2 Formatdehydrogenase
1.2.1.38 N-Acetyl-γ-glutamylphosphatreductase
1.2.1.41 Glutamat-5-semialdehyddehydrogenase
1.2.1.9 Glyceraldehyd-3-phosphatdehydrogenase (NADP+)
1.2.7.1 Pyruvatsynthase
1.2.99.2 Kohlenmonoxiddehydrogenase
1.3.1.12 Prephenatdehydrogenase
1.3.1.26 Dihydrodipicolinatreductase
1.3.1.43 Cyclohexadienyldehydrogenase
1.3.3.1 Dihydroorotatoxidase
1.3.99.1 Succinatdehydrogenase
1.3.99.3 Acyl-CoA-Dehydrogenase
1.4.1.13 Glutamatsynthase (NADPH)
1.4.1.14 Glutamatsynthase (NADH)
1.4.1.4 Glutamatdehydrogenase (NADP+)
1.4.3.16 L-Aspartatoxidase
1.4.7.1 Glutamatsynthase (ferredoxin)
1.5.1.2 Pyrrolin-5-carboxylatreductase
1.5.1.3 Dihydrofolatreductase
1.5.1.5 Methylenetetrahydrofolatdehydrogenase (NADP+)
1.6.4.5 Thioredoxinreductase (NADPH)
1.6.6.4 Nitritreductase (NAD(P)H)
1.6.6.8 GMP-Reductase
1.6.99.2 NAD(P)H-Dehydrogenase (Chinon)
1.7.7.1 Ferredoxin-Nitrit-Reductase
1.7.7.2 Ferredoxin-Nitrat-Reductase
1.8.4.6 Protein-Methianin-S-oxidreductase
1.8.99.2 Adenylylsulfatreductase
1.97.1.4 [Pyruvatformatlyase]-aktivierendes Enzym
1.97.4.1
2.1.1.104 Caffeoyl-CoA-O-Methyltransferase
2.1.1.107 Uroporphyrin-III c-Methyltransferase
2.1.1.13 5-Methyltetrahydrofolat-Homocystein S- methyltransferase
2.1.1.31 tRNA-(Guanin-N1-)-methyltransferase
2.1.1.37 DANN-(Cytosin-5-)-methyltransferase
2.1.1.45 Thymidylatsynthase
2.1.1.63 methylierte-DNA-Protein-Cystein-S- Methyltransferase
2.1.1.72 Sequenz-spezifische DANN-Methyltransferase (Adenin-spezifisch)
2.1.1.73 Sequenz-spezifische DANN-Methyltransferase (Cytosin-spezifisch)
2.1.1.79 Cyclopropan-Fettsäure-Acyl-phospholipidsynthase
2.1.1.80 Protein-Glutamat-O-methyltransferase
2.1.2.1 Glycinhydroxymethyltransferase
2.1.2.11 3-Methyl-2-oxobutanoathydroxymethyltransferase
2.1.2.2 Phosphoribosylglycinamid-Formyltransferase
2.1.2.3 Phosphoribosylaminoimidazolcarboxamid- Formyltransferase
2.1.2.5 Glutamat-Formiminotransferase
2.1.2.9 Methionyl-tRNA-Formyltransferase
2.1.3.2 Aspartatcarbamoyltransferase
2.1.3.3 Ornithincarbamoyltransferase
2.2.1.1 Transketolase
2.2.1.2 Transaldolase
2.3.1.117 2,3,4,5-Tetrahydropyridin-2-carboxylat-N- succinyltransferase
2.3.1.128 ribosomales Protein-Alanin-N-Acetyltransferase
2.3.1.18 Galactosid-O-Acetyltransferase
2.3.1.19 Phosphatbutyryltransferase
2.3.1.28 Chloramphenicol-O-Acetyltransferase
2.3.1.30 Serin O-Acetyltransferase
2.3.1.35 Glutamat-N-Acetyltransferase
2.3.1.39 [Acyl-Carrierprotein]-S-Malonyltransferase
2.3.1.41 3-Oxoacyl-[Acyl-Carrierprotein]-synthase
2.3.1.46 Homoserin O-Succinyltransferase
2.3.1.51 1-Acylglycerol-3-phosphat-O-Acyltransferase
2.3.1.54 Format c-Acetyltransferase
2.3.1.8 Phosphatacetyltransferase
2.3.1.85 Fettsäuresynthase
2.3.1.9 Acetyl-CoA-C-Acetyltransferase
2.4.1.1 Phosphorylase
2.4.1.10 Levansucrase
2.4.1.117 Dolichylphosphat-β-glucosyltransferase
2.4.1.12 Cellulosesynthase (UDP-bildend)
2.4.1.17 Glucuronosyltransferase
2.4.1.19 Cyclomaltodextrin-Glucanotransferase
2.4.1.21 Stärkesynthase
2.4.1.46 1,2-Diacylglycerol-3-β-galactosyltransferase
2.4.1.83 Dolichylphosphat-β-D-mannosyltransferase
2.4.2.1 Purin-Nucleosid-Phosphorylase
2.4.2.10 Orotat-Phosphoribosyltransferase
2.4.2.14 Amidophosphoribosyltransferase
2.4.2.17 ATP-Phosphoribosyltransferase
2.4.2.18 Anthranilat-Phosphoribosyltransferase
2.4.2.2 Pyrimidin-Nucleosid-Phosphorylase
2.4.2.21 Nicotinat-Nucleotid-dimethylbenzimidazol- Phosphoribosyl
2.4.2.29 Queuin-tRNA-Ribosyltransferase
2.4.2.4 Thymidinphosphorylase
2.4.2.7 Adeninphosphoribosyltransferase
2.4.2.8 Hypoxanthin-Phosphoribosyltransferase
2.4 : 2.9 Uracil-Phosphoribosyltransferase
2.4.99.1 β-Galactosid-α-2,6-sialyltransferase
2.5.1.10 Geranyltranstransferase
2.5.1.15 Dihydropteroatsynthase
2.5.1.16 Spermidinsynthase
2.5.1.19 3-Phosphoshikimat-1-carboxyvinyltransferase
2.5.1.29 Farnesyltranstransferase
2.5.1.3 Thiaminphosphatpyrophosphorylase
2.5.1.31 Di-trans,poly-cis-decaprenylcistransferase
2.5.1.6 Methionin-Adenosyltransferase
2.5.1.7 UDP-N-Acetylglucosamin-1-carboxyvinyltransferase
2.5.1.9 Riboflavinsynthase
2.6.1.1 Aspartattransaminase
2.6.1.11 Acetylornithintransaminase
2.6.1.16 Glutamin-Fructose-6-phosphattransaminase (isomerisierend)
2.6.1.19 4-Aminobutyrattransaminase
2.6.1.21 D-Alanintransaminase
2.6.1.42 verzweigte Ketten-Aminosäure-Transaminase
2.6.1.44 Alanin-Glyoxylattransaminase
2.6.1.45 Serin-Glyoxylattransaminase
2.6.1.51 Serin-Pyruvattransaminase
2.6.1.62 Adenosylmethionin-8-amino-7- oxononanoattransaminase
2.6.1.9 Histidinolphosphattransaminase
2.7.1.11 6-Phosphofructokinase
2.7.1.17 Xylulokinase
2.7.1.21 Thymidinkinase
2.7.1.25 Adenylylsulfatkinase
2.7.1.30 Glycerolkinase
2.7.1.37 Proteinkinase
2.7.1.39 Homoserinkinase
2.7.1.4 Fructokinase
2.7.1.40 Pyruvatkinase
2.7.1.49 Hydroxymethylpyrimidinkinase
2.7.1.50 Hydroxyethylthiazolkinase
2.7.1.56 1-Phosphofructokinase
2.7.1.6 Galactokinase
2.7.1.69 Protein-N(pai)-Phosphohistidin-Zucker- Phosphotransferase
2.7.1.71 Shikimatkinase
2.7.2.1 Acetatkinase
2.7.2.11 Glutamat-5-kinase
2.7.2.3 Phosphoglyceratkinase
2.7.2.4 Aspartatkinase
2.7.2.7 Butyratkinase
2.7.2.8 Acetylglutamatkinase
2.7.3.3 Argininkinase
2.7.3.9 Phosphoenolpyruvat-Protein-Phosphotransferase
2.7.4.1 Polyphosphatkinase
2.7.4.14 Cytidylatkinase
2.7.4.3 Adenylatkinase
2.7.4.8 Guanylatkinase
2.7.6.1 Ribosephosphat-Pyrophosphokinase
2.7.6.2 Thiaminpyrophosphokinase
2.7.6.3 2-Amino-4-hydroxy-6-hydroxymethyldihydropteridin- Pyro-
2.7.6.5 GTP-Pyrophosphokinase
2.7.7.10 UTP-Hexose-1-phosphat-Uridylyltransferase
2.7.7.12 UDP-Glucose-Hexose-1-phosphat-Uridylyltransferase
2.7.7.13 Mannose-1-phosphat-Guanylyltransferase
2.7.7.19 Polynucleotid-Adenylyltransferase
2.7.7.24 Glucose-1-phosphat-Thymidylyltransferase
2.7.7.25 tRNA-Adenylyltransferase
2.7.7.27 Glucose-1-phosphat-Adenylyltransferase
2.7.7.4 Sulfat-Adenylyltransferase
2.7.7.6 DNA-bezogene RNA-Polymerase
2.7.7.7 DNA-bezogene DNA-Polymerase
2.7.7.8 Polyribonucleotid-Nucleotidyltransferase
2.7.7.9 UTP-Glucose-1-phosphat-Uridylyltransferase
2.7.8.13 Phospho-N-Acetylmuramoylpentapeptidtransferase
2.7.8.5 CDP-Diacylglycerol-glycerol-3-phsophat-3- phosphatidyltransferase
2.7.8.6 Undecaprenylphosphat-Galactosephosphotransferase
2.7.8.7 Holo-[Acyl-Carrierprotein]-synthase
2.7.8.8 CDP-Diacylglycerol-Serin-O-phosphatidyltransferase
2.7.9.2 Pyruvat-Wasser-Dikinase
3.1.1.11 Pectinesterase
3.1.1.2 Arylesterase
3.1.1.29 Aminoacyl-tRNA-Hydrolase
3.1.1.3 Triacylglycerollipase
3.1.1.5 Lisophospholipase
3.1.1.61 Protein-Glutamat-Methylesterase
3.1.11.5 Exodesoxyribonuclease V
3.1.11.6 Exodesoxyribonuclease VII
3.1.2.14 Oleoyl-[Acyl-Carrierprotein]-hydrolase
3.1.2.6 Hydroxyacylglutathionhydrolase
3.1.21.4 Typ II Sequenz-spezifische Desoxyribonuclease
3.1.26.3 Ribonuclease III
3.1.26.4 Kälberthymus-Ribonuclease H
3.1.27.1 Ribonuclease T2
3.1.3.11 Fructosebisphosphatase
3.1.3.15 Histidinolphosphatase
3.1.3.16 Phosphoproteinphosphatase
3.1.3.18 Phosphoglycolatphosphatase
3.1.3.48 Protein-Tyrosinphosphatase
3.1.3.5 5'-Nucleotidase
3.1.4.1 Phosphodiesterase I
3.1.4.14 [Acyl-Carrierprotein]-Phosphodiesterase
3.1.4.16 2',3'-zyclisches Nucleotid 2'-Phosphodiesterase
3.1.4.3 Phospholipase c
3.1.4.46 Glycerophosphodiester-Phosphodiesterase
3.2.1.1 α-Amylase
3.2.1.135 Neopullulanase
3.2.1.14 Chitinase
3.2.1.17 Lysozyme
3.2.1.20 α-Glucosidase
3.2.1.21 β-Glucosidase
3.2.1.23 β-Galactosidase
3.2.1.25 β-Mannosidase
3.2.1.26 β-Fructofuranosidase
3.2.1.37 Xylan-1,4-β-xylosidase
3.2.1.39 Glucan-endo-1,3-β-D-glucosidase
3.2.1.4 Cellulase
3.2.1.41 α-Dextrin-endo-1,6-alpha-glucosidase
3.2.1.52 β-N-Acetylhexosaminidase
3.2.1.54 Cyclomaltodextrinase
3.2.1.55 α-N-Arabinofuranosidase
3.2.1.58 Glucan-1,3-β-glucosidase
3.2.1.60 Glucan-1,4-α-maltotetraohydrolase
3.2.1.65 Levanase
3.2.1.7 Inulinase
3.2.1.73 Licheninase
3.2.1.74 Glucan-1,4-β-glucosidase
3.2.1.78 Mannan-endo-1,4-β-mannosidase
3.2.1.8 Endo-1,4-β-xylanase
3.2.1.82 Exo-poly-α-galacturonosidase
3.2.1.85 6-Phospho-β-galactosidase
3.2.1.86 6-Phospho-β-glucosidase
3.2.1.91 Cellulose-1,4-β-cellobiosidase
3.2.2.17 Desoxyribodipyrimidinndonucleosidase
3.2.2.20 DNA-3-methyladeninglycosidaseI
3.2.2.24 ADP-Ribosyl-[Distickstoffreductase]-hydrolase
3.3.2.3 Epoxidhydrolase
3.4.13.9 X-Pro-Dipeptidase
3.4.16.4 Serin-Typ D-Ala,D-Ala-Carboxypeptidase
3.4.21.53 Endopeptidase Ia
3.4.21.62 Subtilisin
3.4.23.33 Xanthomonapepsin
3.4.23.36 Signalpeptidase II
3.4.24.27 Thermolysin
3.4.24.28 Bacillolysin
3.4.24.55 Pitrilysin
3.4.24.57 O-Syaloglycoproteinendopeptidase
3.4.99.36 Leitpeptidase
3.4.99.41 mitochondriale Prozessierungs-Peptidase
3.5.1.1 Asparaginase
3.5.1.18 Succinyl-diaminopimelatdesuccinylase
3.5.1.25 N-Acetylglucosamin-6-phosphatdeacetylase
3.5.1.27 N-Formylmethionylaminoacyl-tRNA-Deformylase
3.5.1.28 N-Acetylmuramoyl-L-Alanin-Amidase
3.5.1.38 Glutamin-(Asparagin-)ase
3.5.2.3 Dihydroorotase
3.5.2.6 β-Lactamase
3.5.3.1 Arginase
3.5.4.10 IMP-Cyclohydrolase
3.5.4.12 dCMP-Deaminase
3.5.4.16 GTP-Cyclohydrolase I
3.5.4.19 Phosphoribosyl-AMP-Cyclohydrolase
3.5.4.2 Adenindeaminase
3.5.4.23 Blasticidin-S-Deaminase
3.5.4.25 GTP-Cyclohydrolase II
3.5.4.4 Adenosindeaminase
3.5.4.5 Cytidindeaminase
3.5.4.9 Methenyltetrahydrofolatcyclohydrolase
3.6.1.17 bis(5'-Nucleosyl)-tetraphosphatase (asymmetrisch)
3.6.1.23 dUTP-Pyrophosphatase
3.6.1.3 Adenosintriphosphatase
3.6.1.31 Phosphoribosyl-ATP-Pyrophosphatase
3.6.1.34 H+-transportierende ATP-Synthase
3.6.1.36 H+/K+ austauschende ATPase
3.6.1.38 Ca2+-transportierende ATPase
3.6.1.41 bis(5'-Nucleosyl)-tetraphosphatase (symmetrisch)
3.6.1.7 Acylphosphatase
3.6.1.9 Nucleotidpyrophosphatase
3.8.1.3 Haloacetatdehalogenase
4.1.1.11 Aspartat-1-decarboxylase
4.1.1.18 Lysindecarboxylase
4.1.1.19 Arginindecarboxylase
4.1.1.20 Diaminopimelatdecarboxylase
4.1.1.21 Phosphoribosylaminoimidazolcarboxylase
4.1.1.23 Orotidin-5'-phosphatdecarboxylase
4.1.1.48 Indol-3-glycerolphosphatsynthase
4.1.1.5 Acetolactatdecarboxylase
4.1.1.50 Adenosylmethionindecarboxylase
4.1.1.65 Phosphatidylserindecarboxylase
4.1.2.13 Fructosebisphosphataldolase
4.1.2.14 2-Dehydro-3-desoxyphosphogluconat-Aldolase
4.1.2.15 2-Dehydro-3-desoxyphosphoheptonat-Aldolase
4.1.2.25 Dihydroneopterin-Aldolase
4.1.2.4 Desoxyribosephosphataldolase
4.1.3.12 2-Isopropylmalatsynthase
4.1.3.16 4-Hydroxy-2-oxoglutarat-Aldolase
4.1.3.18 Acetolactatsynthase
4.1.3.21 Homocitratsynthase
4.1.3.27 Anthranilatsynthase
4.2.1.1 Carbonatdehydratase
4.2.1.10 3-Dehydrochinatdehydratase
4.2.1.11 Phosphopyruvathydratase
4.2.1.12 Phosphogluconatdehydratase
4.2.1.13 L-Serindehydratase
4.2.1.17 Enoyl-CoA-Hydratase
4.2.1.19 Imidazolglycerolphosphatdehydratase
4.2.1.2 Fumarathydratase
4.2.1.20 Tryptophansynthase
4.2.1.22 Cystathionin-β-Synthase
4.2.1.24 Porphobilinogensynthase
4.2.1.3 Aconitathydratase
4.2.1.32 L(+)-Tartratdehydratase
4.2.1.33 3-Isopropylmalatdehydratase
4.2.1.36 Homoaconitathydratase
4.2.1.46 dTDP-Glucose-4,6-dehydratase
4.2.1.51 Prephenatdehydratase
4.2.1.52 Dihydrodipicolinatsynthase
4.2.1.7 Altronatdehydratase
4.2.1.74 lange Ketten-enoyl-CoA-Hydratase
4.2.1.75 Uroporphyrinogen-III-Synthase
4.2.1.8 Mannonatdehydratase
4.2.1.9 Dihydroxysäuredehydratase
4.2.2.2 Pectatlyase
4.2.99.11 Methylglyoxalsynthase
4.2.99.18 DNA-(apurinische oder apyrimidinische Sequenz)- lyase
4.2.99.2 Threoninsynthase
4.2.99.8 Cysteinsynthase
4.3.1.1 Aspartat-Ammoniak-Lyase
4.3.1.4 Formiminotetrahydrofolatcyclodeaminase
4.3.1.7 Ethanolamin-Ammoniak-Lyase
4.3.1.8 Hydroxymethylbilansynthase
4.3.2.1 Argininosuccinatlyase
4.3.2.2 Adenylsuccinatlyase
4.4.1.8 Cystathionin-beta-lyase
4.6.1.3 3-Dehydrochinatsynthase
4.6.1.4 Chorismatsynthase
5.1.1.1 Alaninracemase
5.1.1.3 Glutamatracemase
5.1.1.7 Diaminopimaratepimerase
5.1.2.3 3-Hydroxybutyryl-CoA-Epimerase
5.1.3.1 Ribulosephosphat-3-epimerase
5.1.3.13 dTDP-4-Dehydrorhamnose-3,5-epimerase
5.1.3.14 UDP-N-Acetylglucosamin-2-epimerase
5.1.3.2 UDP-Glucose-4-epimerase
5.1.3.3 Aldose-1-epimerase
5.1.3.4 L-Ribulosephosphat-4-epimerase
5.2.1.8 Peptidylprolylisomerase
5.3.1.1 Triosephosphatisomerase
5.3.1.10 Glucosamin-6-phosphatisomerase
5.3.1.12 Glucuronatisomerase
5.3.1.16 N-(5'-Phospho-D-ribosylformimino)-5-amino-1-
5.3.1.24 Phosphoribosylanthranilatisomerase
5.3.1.4 L-Arabinoseisomerase
5.3.1.6 Ribose-5-phosphatepimerase
5.3.1.8 Mannose-6-phosphatisomerase
5.3.1.9 Glucose-6-phosphatisomerase
5.3.3.8 Dodecenoyl-CoA-Δ-isomerase
5.4.2.1 Phosphoglyceratmutase
5.4.2.6 β-Phosphoglucomutase
5.4.2.7 Phosphopentomutase
5.4.3.8 Glutamat-1-semialdehyd-2,1-Aminomutase
5.4.99.12 tRNA-Pseudouridinsynthase I
5.4.99.5 Chorismatmutase
5.5.1.1 Muconatcycloisomerase
5.99.1.2 DNA-Topoisomerase
5.99.1.3 DNA-Topoisomerase (ATP-hydrolysierend)
6.1.1.1 Tyrosin-tRNA-Ligase
6.1.1.10 Methionin-tRNA-Ligase
6.1.1.11 Serin-tRNA-Ligase
6.1.1.12 Aspartat-tRNA-Ligase
6.1.1.14 Glycin-tRNA-Ligase
6.1.1.15 Prolin-tRNA-Ligase
6.1.1.16 Cystein-tRNA-Ligase
6.1.1.17 Glutamat-tRNA-Ligase
6.1.1.19 Arginin-tRNA-Ligase
6.1.1.2 Tryptophan-tRNA-Ligase
6.1.1.20 Phenylalanin-tRNA-Ligase
6.1.1.21 Histidin-tRNA-Ligase
6.1.1.22 Asparagin-tRNA-Ligase
6.1.1.3 Threonin-tRNA-Ligase
6.1.1.4 Leucin-tRNA-Ligase
6.1.1.5 Isoleucin-tRNA-Ligase
6.1.1.6 Lysin-tRNA-Ligase
6.1.1.7 Alanin-tRNA-Ligase
6.1.1.9 Valin-tRNA-Ligase
6.3.1.2 Glutamat-Ammoniakligase
6.3.2.1 Pantoat-β-Alaninligase
6.3.2.13 UDP-N-Acetylmuramoylalnyl-D-glutamat-2,6- diaminopimelatligase
6.3.2.15 UDP-N-Acetylmuramoylalanyl-D-glutamyl-2,6-
6.3.2.17 Tetrahydrofolylpolyglutamatsynthase
6.3.2.2 Glutamat-Cysteinligase
6.3.2.4 D-Alanin-D-Alaninligase
6.3.2.6 Phosphoribosylaminoimidazolsuccinocarboxyamidsynthase
6.3.2.8 UDP-N-Acetylmuramat-Alaninligase
6.3.2.9 UDP-N-Acetylmuramoylalanin-D-Glutamatligase
6.3.3.1 Phosphoribosyl-Formylglycinamidincycloligase
6.3.3.2 5-Formyltetrahydrofolatcycloligase
6.3.3.3 Dethiobiotinsynthase
6.3.4.13 Phosphoribosylamin-Glycinligase
6.3.4.14 Biotincarboxylase
6.3.4.15 Biotin-[acetyl-CoA-carboxylase]-ligase
6.3.4.2 CTP-Synthase
6.3.4.3 Format-Tetrahydrofolatligase
6.3.4.4 Adenylosuccinatsynthase
6.3.4.5 Argininosuccinatsynthase
6.3.5.2 GMP-Synthase (Glutamin-hydrolysierend)
6.3.5.3 Phosphoribosylformylglycinamidinsynthase
6.3.5.4 Asparaginsynthase (Glutamin-hydrolysierend)
6.3.5.5 Carbamoylphosphatsynthase (Glutamin-hydrolysierend)
6.4.1.1 Pyruvatcarboxylase
6.4.1.2 Acetyl-CoA-Carboxylase
6.5.1.2 DNA-Ligase (NAD+). Enzyme aus Saccharomyces cerevisiae mit EC-Nummer 1.1.1.1 Alkoholdehydrogenase
1.1.1.100 3-Oxoacyl-[Acyl-Carrierprotein]-reductase
1.1.1.14 L-Iditol-2-dehydrogenase
1.1.1.145 3-β-Hydroxy-A5-steroiddehydrogenase
1.1.1.179 D-Xylose-1-dehydrogenase (NADP+)
1.1.1.188 Prostaglandin F-Synthase
1.1.1.195 Cinnamylalkoholdehydrogenase
1.1.1.2 Alkoholdehydrogenase(NADP+)
1.1.1.202 1,3-Propandioldehydrogenase
1.1.1.205 IMP-Dehydrogenase
1.1.1.21 Aldehydreductase
1.1.1.23 Histidinoldehydrogenase
1.1.1.244 Methanoldehydrogenase
1.1.1.25 Shikimat-5-dehydrogenase
1.1.1.27 L-Lactatdehydrogenase
1.1.1.29 Glyceratdehydrogenase
1.1.1.3 Homoserindehydrogenase
1.1.1.34 Hydroxymethylglutaryl-CoA-Reductase (NADPH)
1.1.1.35 3-Hydroxyacyl-CoA-Dehydrogenase
1.1.1.36 Acetoacetyl-CoA-Reductase
1.1.1.37 Malatdehydrogenase
1.1.1.38 Malatdehydrogenase (Oxaloacetat-decarboxylierend)
1.1.1.40 Malatdehydrogenase (Oxaloacetat- decarboxylierend)(NADP+)
1.1.1.41 Isocitratdehydrogenase (NAD+)
1.1.1.42 Isocitratdehydrogenase (NADP+)
1.1.1.44 Phosphogluconatdehydrogenase (decarboxylierend)
1.1.1.49 Glucose-6-phosphat-1-dehydrogenase
1.1.1.57 Fructuronatreductase
1.1.1.62 Estradiol-17β-dehydrogenase
1.1.1.73 Octanoldehydrogenase
1.1.1.77 Lactaldehydreductase
1.1.1.8 Glycerol-3-phosphatdehydrogenase (NAD+)
1.1.1.81 Hydroxypyruvatreductase
1.1.1.85 3-Isopropylmalatdehydrogenase
1.1.1.86 Ketosäure-Reductoisomerase
1.1.1.88 Hydroxymethylglutaryl-CoA-Reductase
1.1.1.9 D-Xylulosereductase
1.1.1.91 Aryl-Alkoholdehydrogenase (NADP+)
1.1.1.95 Phosphoglyceratdehydrogenase
1.1.2.3 L-Lactatdehydrogenase (Cytochrom)
1.1.2.4 D-Lactatdehydrogenase (Cytochrom)
1.1.3.15 (S)-2-Hydroxysäureoxidase
1.1.3.24 L-Galactonolactoneoxidase
1.1.3.8 L-Gulonolactoneoxidase
1.1.99.5 Glycerol-3-phosphatdehydrogenase
1.10.2.2 Ubichinol-Cytochrom c-Reductase
1.10.3.1 Catecholoxidase
1.10.3.2 Laccase
1.11.1.11 L-Ascorbatperoxidase
1.11.1.12 Phospholipid-Hydroperoxid-Glutathionperoxidase
1.11.1.5 Cytochrom c-Peroxidase
1.11.1.6 Katalase
1.11.1.9 Glutathionperoxidase
1.12.1.2 Hydrogendehydrogenase
1.13.11.32 2-Nitropropandioxygenase
1.13.11.42 Indolaminpyrrole-2,3-dioxygenase
1.13.11.6 3-Hydroxyanthranilat-3,4-dioxygenase
1.14.11.14 6β-Hydroxyhyoscyaminepoxidase
1.14.13.8 Dimethylanilinmonooxygenase (N-Oxid-bildend)
1.14.99.5 Stearoyl-CoA-Desaturase
1.14.99.7 Squalenemonooxygenase
1.15.1.1 Superoxiddismutase
1.17.4.1 Ribonucleosiddiphosphatreductase
1.18.1.2 Ferredoxin-NADP+-Reductase
1.18.99.1 Hydrogenase
1.2.1.1 Formaldehyddehydrogenase (Glutathion)
1.2.1.11 Aspartatsemialdehyd-Dehydrogenase
1.2.1.12 Glyceraldehyd-3-phosphatdehydrogenase (phosphorylierend)
1.2.1.16 Succinatsemialdehyddehydrogenase (NAD(P)+)
1.2.1.2 Formatdehydrogenase
1.2.1.3 Aldehyddehydrogenase (NAD+)
1.2.1.31 L-Aminoadipatsemialdehyddehydrogenase
1.2.1.38 N-Acetyl-γ-glutamylphosphatreductase
1.2.1.41 Glutamat-5-semialdehyddehydrogenase
1.2.1.5 Aldehyddehydrogenase (NAD(P)+)
1.2.1.8 Betain-Aldehyddehydrogenase
1.2.4.1 Pyruvatdehydrogenase (Lipoamid)
1.2.4.2 Oxoglutaratdehydrogenase (Lipoamid)
1.3.1.13 Prephenatdehydrogenase (NADP+)
1.3.1.23 Cholestenone-5β-reductase
1.3.1.29 cis-1,2-Dihydro-1,2- dihydroxynaphthalendehydrogenase
1.3.1.34 2,4-Dienoyl-CoA-Reductase (NADPH)
1.3.1.9 Enoyl-[Acyl-Carrierprotein]-reductase (NADH)
1.3.3.1 Dihydroorotatoxidase
1.3.3.3 Coproporphyrinogenoxidase
1.3.3.4 Protoporphyrinogenoxidase
1.3.3.6 Acyl-CoA-Oxidase
1.3.5.1 Succinatdehydrogenase (Ubichinon)
1.3.99.1 Succinatdehydrogenase
1.4.1.13 Glutamatsynthase (NADPH)
1.4.1.14 Glutamatsynthase (NADH)
1.4.1.2 Glutamatdehydrogenase
1.4.1.4 Glutamatdehydrogenase (NADP+)
1.4.3.16 L-Aspartatoxidase
1.4.3.5 Pyridoxaminphosphatoxidase
1.4.4.2 Glycindehydrogenase (decarboxylierend)
1.4.7.1 Glutamatsynthase (ferredoxin)
1.5.1.10 Saccharopindehydrogenase (NADP+, L-Glutamat bildend)
1.5.1.12 1-Pyrrolin-5-carboxylatdehydrogenase
1.5.1.15 Methylentetrahydrofolatdehydrogenase (NAD+)
1.5.1.2 Pyrrolin-5-carboxylatreductase
1.5.1.20 Methylentetrahydrofolatreductase (NADPH)
1.5.1.3 Dihydrofolatreductase
1.5.1.5 Methylentetrahydrofolatdehydrogenase (NADP+)
1.5.1.7 Saccharopindehydrogenase (NAD+, L-lysin bildend)
1.5.1.8 Saccharopindehydrogenase (NADP+, L-lysin bildend)
1.5.99.8 Prolindehydrogenase
1.6.2.2 Cytochrom b5-Reductase
1.6.2.4 NADPH-Ferrihämoprotein-Reductase
1.6.4.2 Glutathionreductase (NADPH)
1.6.4.5 Thioredoxinreductase (NADPH)
1.6.5.3 NADH-Dehydrogenase (Ubichinon)
1.6.5.5 NADPH : Chinonreductase
1.6.6.1 Nitratreductase (NADH)
1.6.99.1 NADPH-Dehydrogenase
1.7.99.5 5,10-Methylentetrahydrofolatreductase (fadh2)
1.7.99.7 Stickoxidreductase
1.8.1.2 Sulfitreductase (NADPH)
1.8.1.4 Dihydrolipoamiddehydrogenase
1.8.7.1 Sulfitreductase (Ferredoxin)
1.8.99.4
1.9.3.1 Cytochrom c-Oxidase
2.1.1.107 Uroporphyrin-III c-Methyltransferase
2.1.1.14 5-Methyltetrahydropteroyltriglutamat-Homocystein
2.1.1.17 Phosphatidylethanolamin-N-methyltransferase
2.1.1.41 24-Sterol c-methyltransferase
2.1.1.45 Thymidylatsynthase
2.1.1.63 methylierte-DNA-Protein-Cystein-S- Methyltransferase
2.1.1.64 3-Demethylubichinon-9-3-O-methyltransferase
2.1.1.71 Phosphatidyl-N-methylethanolamin-N- methyltransferase
2.1.1.98 Diphthinsynthase
2.1.2.1 Glycinhydroxymethyltransferase
2.1.2.10 Aminomethyltransferase
2.1.2.11 3-Methyl-2-oxobutanoathydroxymethyltransferase
2.1.2.2 Phosphoribosylglycinamid-Formyltransferase
2.1.2.3 Phosphoribosylaminoimidazolcarboxamid- Formyltransferase
2.1.2.9 Methionyl-tRNA-Formyltransferase
2.1.3.2 Aspartatcarbamoyltransferase
2.1.3.3 Ornithincarbamoyltransferase
2.2.1.1 Transketolase
2.2.1.2 Transaldolase
2.3.1.12 Dihydrolipoamid S-Acetyltransferase
2.3.1.128 ribosomales Protein-Alanin N-Acetyltransferase
2.3.1.15 Glycerol-3-phosphat-O-Acyltransferase
2.3.1.16 Acetyl-CoA-C-Acyltransferase
2.3.1.18 Galactosid O-Acetyltransferase
2.3.1.21 Carnitin O-palmitoyltransferase
2.3.1.31 Homoserin O-Acetyltransferase
2.3.1.35 Glutamat N-Acetyltransferase
2.3.1.37 5-Aminolevulinatsynthase
2.3.1.38 [Acyl-Carrierprotein]-S-Acetyltransferase
2.3.1.39 [Acyl-Carrierprotein]-S-malonyltransferase
2.3.1.41 3-Oxoacyl-[Acyl-Carrierprotein]-synthase
2.3.1.43 Phosphatidylcholin-sterol-O-Acyltransferase
2.3.1.47 8-Amino-7-oxononanoatsynthase
2.3.1.48 Histon-Acetyltransferase
2.3.1.50 Serin-C-palmitoyltransferase
2.3.1.51 1-Acylglycerol-3-phosphat-O-Acyltransferase
2.3.1.6 Cholin O-Acetyltransferase
2.3.1.61 Dihydrolipoamid-S-Succinyltransferase
2.3.1.7 Carnitin-O-Acetyltransferase
2.3.1.84 Alcohol-O-Acetyltransferase
2.3.1.85 Fettsäuresynthase
2.3.1.86 Fettsäure-Acyl-CoA-Synthase
2.3.1.9 Acetyl-CoA-C-Acetyltransferase
2.3.1.97 Glycylpeptid-N-Tetradecanoyltransferase
2.3.2.2 γ-glutamyltransferase
2.3.2.5 Glutaminylpeptidcyclotransferase
2.3.2.8 Arginyltransferase
2.4.1.1 Phosphorylase
2.4.1.109 Dolichyl-Phosphat-Mannose-Protein- Mannosyltransferase
2.4.1.11 Glycogen(Stärke)synthase
2.4.1.117 Dolichylphosphat-β-glucosyltransferase
2.4.1.119 Dolichyldiphosphooligosaccharid- Proteinglycosyltransferase
2.4.1.131 Glycolipid-2-α-mannosyltransferase
2.4.1.15 α,α-Trehalosephosphatsynthase (UDP-bildend)
2.4.1.16 Chitinsynthase
2.4.1.18 1,4-α-Glucan verzweigendes Enzym
2.4.1.186 Glycogenin-Glucosyltransferase
2.4.1.25 4-α-Glucanotransferase
2.4.1.34 1,3-β-Glucansynthase
2.4.1.83 Dolichylphosphat-β-D-mannosyltransferase
2.4.2.1 Purin-Nucleosid-Phosphorylase
2.4.2.10 Orotat-Phosphoribosyltransferase
2.4.2.11 Nicotinat-Phosphoribosyltransferase
2.4.2.14 Amidophosphoribosyltransferase
2.4.2.17 ATP-Phosphoribosyltransferase
2.4.2.18 Anthranilat-Phosphoribosyltransferase
2.4.2.19 Nicotinat-Nucleotid-Pyrophosphorylase (carboxylierend)
2.4.2.28 5'-Methylthioadenosinphosphorylase
2.4.2.29 Queuin-tRNA-Ribosyltransferase
2.4.2.7 Adeninphosphoribosyltransferase
2.4.2.9 Uracil-Phosphoribosyltransferase
2.5.1.1 Dimethylallyltranstransferase
2.5.1.10 Geranyltranstransferase
2.5.1.15 Dihydropteroatsynthase
2.5.1.16 Spermidinsynthase
2.5.1.18 Glutathiontransferase
2.5.1.19 3-Phosphoshikimat-1-carboxyvinyltransferase
2.5.1.21 Farnesyldiphosphat-Farnesyltransferase
2.5.1.29 Farnesyltranstransferase
2.5.1.3 Thiaminphosphatpyrophosphorylase
2.5.1.31 Di-trans,poly-cis-Decaprenylcistransferase
2.5.1.32 Geranylgeranyldiphosphat-Geranylgeranyltransferase
2.5.1.33 trans-Pentaprenyltranstransferase
2.5.1.6 Methionin-Adenosyltransferase
2.5.1.8 tRNA-Isopentenyltransferase
2.5.1.9 Riboflavinsynthase
2.6.1.1 Aspartattransaminase
2.6.1.11 Acetylornithintransaminase
2.6.1.13 Ornithin-Oxosäure-Transaminase
2.6.1.14 Asparagin-Oxosäure-Transaminase
2.6.1.16 Glutamin-Fructose-6-phosphattransaminase (isomerisierend)
2.6.1.19 4-Aminobutyrattransaminase
2.6.1.2 Alanintransaminase
2.6.1.36 L-Lysin-6-transaminase
2.6.1.42 verzweigte Ketten-Aminosäure-Transaminase
2.6.1.45 Serin-Glyoxylattransaminase
2.6.1.52 Phosphoserintransaminase
2.6.1.62 Adenosylmethionin-8-amino-7- oxononanoattransaminase
2.6.1.64 Glutamin-Phenylpyruvattransaminase
2.6.1.7 Kynurenin-Oxoglutarattransaminase
2.6.1.9 Histidinolphosphattransaminase
2.7.1.1 Hexokinase
2.,7.1.105 6-Phosphofructo-2-kinase
2.7.1.109 [Hydroxymethylglutaryl-CoA-Reductase(NADPH)]- kinase
2.7.1.11 6-Phosphofructokinase
2.7.1.112 Protein-Tyrosinkinase
2.7.1.115 [3-Methyl-2-oxobutanoatdehydrogenase (Lipoamid)]- kinase
2.7.1.117 Myosin-leichte Ketten-Kinase
2.7.1.12 Gluconokinase
2.7.1.123 Ca2+/Calmodulin-abhängige Proteinkinase
2.7.1.128 [Acetyl-CoA-Carboxylase]-kinase
2.7.1.129 Myosin-schwere Ketten-Kinase
2.7.1.135 τ-Proteinkinase
2.7.1.137 1-Phosphatidylinositol 3-kinase
2.7.1.141 [RNA-Polymerase]-Untereinheit-Kinase
2.7.1.15 Ribokinase
2.7.1.17 Xylulokinase
2.7.1.2 Glucokinase
2.7.1.20 Adenosinkinase
2.7.1.25 Adenylylsulfatkinase
2.7.1.30 Glycerolkinase
2.7.1.32 Cholinkinase
2.7.1.35 Pyridoxalkinase
2.7.1.36 Mevalonatkinase
2.7.1.37 Proteinkinase
2.7.1.38 Phosphorylasekinase
2.7.1.39 Homoserinkinase
2.7.1.40 Pyruvatkinase
2.7.1.47 D-Ribulokinase
2.7.1.48 Uridinkinase
2.7.1.49 Hydroxymethylpyrimidinkinase
2.7.1.50 Hydroxyethylthiazolkinase
2.7.1.6 Galactokinase
2.7.1.67 1-Phosphatidylinositol 4-kinase
2.7.1.71 Shikimatkinase
2.7.1.99 [Pyruvatdehydrogenase (lipoamid)]-kinase
2.7.2.11 Glutamat-5-kinase
2.7.2.3 Phosphoglyceratkinase
2.7.2.4 Aspartatkinase
2.7.2.8 Acetylglutamatkinase
2.7.4.10 Nucleosidtriphosphatadenylatkinase
2.7.4.14 Cytidylatkinase
2.7.4.2 Phosphomevalonatkinase
2.7.4.3 Adenylatkinase
2.7.4.6 Nucleosiddiphosphatkinase
2.7.4.8 Guanylatkinase
2.7.4.9 DTMP-Kinase
2.7.6.1 Ribosephosphatpyrophosphokinase
2.7.6.2 Thiaminpyrophosphokinase
2.7.7.10 UTP-Hexose-1-phosphat-Uridylyltransferase
2.7.7.12 UDP-Glucose-Hexose-1-phosphat-Uridylyltransferase
2.7.7.13 Mannose-1-phosphat-Guanylyltransferase
2.7.7.14 Ethanolaminphosphatcytidylyltransferase
2.7.7.15 Cholinphosphatcytidylyltransferase
2.7.7.19 Polynucleotid-Adenylyltransferase
2.7.7.25 tRNA-Adenylyltransferase
2.7.7.4 Sulfat-Adenylyltransferase
2.7.7.41 Phosphatidatcytidylyltransferase
2.7.7.5 Sulfat-Adenylyltransferase (ADP)
2.7.7.50 mRNA-Guanylyltransferase
2.7.7.53 ATP-Adenylyltransferase
2.7.7.56 tRNA-Nucleotidyltransferase
2.7.7.6 DNA-bezogene RNA-Polymerase
2.7.7.7 DNA-bezogene DNA-Polymerase
2.7.7.9 UTP-Glucose-1-phosphat-Uridylyltransferase
2.7.8.1 Ethanolaminphosphotransferase
2.7.8.11 CDP-Diacylglycerol-Inositol-3- phosphatidyltransferase
2.7.8.15 UDP-N-Acetylglucosamin-dolichyl-
2.7.8.19 UDP-Glucose-Glycoprotein-Glucosephosphotransferase
2.7.8.2 Diacylglycerolcholinphosphotransferase
2.7.8.5 CDP-Diacylglycerol-Glycerol-3-phsophat-3- phosphatidyltransferase
2.7.8.8 CDP-Diacylglycerolserin-O-phosphatidyltransferase
2.8.1.1 Thiosulfatsulfurtransferase
3.1.1.1 Carboxylesterase
3.1.1.13 Sterolesterase
3.1.1.29 Aminoacyl-tRNA-Hydrolase
3.1.1.3 Triacylglycerollipase
3.1.1.45 Carboxymethylenebutenolidase
3.1.1.5 Lisophospholipase
3.1.13.4 Poly(A)-spezifische Ribonuclease
3.1.2.1 Acetyl-CoA-Hydrolase
3.1.2.15 Ubiquitinthiolesterase
3.1.2.2 Palmitoyl-CoA-Hydrolase
3.1.2.6 Hydroxyacylglutathionhydrolase
3.1.21.2 Desoxyribonuclease IV (Phage T4-induziert)
3.1.26.3 Ribonuclease III
3.1.26.4 Kälberthymus-Ribonuclease H
3.1.26.5 Ribonuclease P
3.1.27.1 Ribonuclease T2
3.1.3.1 alkalische Phosphatase
3.1.3.11 Fructosebisphosphatase
3.1.3.12 Trehalosephosphatase
3.1.3.15 Histidinolphosphatase
3.1.3.16 Phosphoproteinphosphatase
3.1.3.17 [Phosphorylase]phosphatase
3.1.3.2 saure Phosphatase
3.1.3.25 Myoinositol-1(oder 4)-monophosphatase
3.1.3.3 Phosphoserinphosphatase
3.1.3.43 [Pyruvatdehydrogenase (Lipoamid)]-phosphatase
3.1.3.46 Fructose-2,6-bisphosphat-2-phosphatase
3.1.3.48 Protein-Tyrosinphosphatase
3.1.3.7 3'(2'),5'-Bisphosphatnucleotidase
3.1.3.8 3-Phytase
3.1.4.1 Phosphodiesterase I
3.1.4.11 1-Phosphatidylinositol-4,5-bisphosphat- Phosphodiesterase
3.1.4.12 Sphingomyelinphosphodiesterase
3.1.4.17 3,5'-zyclisches Nucleotid-Phosphodiesterase
3.1.4.46 Glycerophosphodiester-Phosphodiesterase
3.1.4.50 Glycoproteinphospholipase D
3.2.1.10 Oligo-1,6-glucosidase
3.2.1.113 Mannosyloligosaccharid-1,2-α-mannosidase
3.2.1.14 Chitinase
3.2.1.15 Polygalacturonase
3.2.1.20 α-glucosidase
3.2.1.24 α-Mannosidase
3.2.1.26 β-Fructofuranosidase
3.2.1.28 α,α-Trehalase
3.2.1.3 Glucan 1,4-α-glucosidase
3.2.1.33 Amylo-1,6-glucosidase
3.2.1.58 Glucan-1,3-β-glucosidase
3.2.1.65 Levanase
3.2.1.7 Inulinase
3.2.1.73 Licheninase
3.2.1.8 Endo-1,4-beta-xylanase
3.2.2.20 DNA-3-methyladeninglycosidaseI
3.3.1.1 Adenosylhomocysteinase
3.3.2.6 Leukotrien A4-Hydrolase
3.4.11.10 baktierelle Leucyl-Aminopeptidase
3.4.11.18 Methionyl-Aminopeptidase
3.4.11.2 Membrane-Alanyl-Aminopeptidase
3.4.11.7 Glutamyl-Aminopeptidase
3.4.11.9 X-Pro-Aminopeptidase
3.4.13.9 X-Pro-Dipeptidase
3.4.14.11 XAA-Pro-Dipeptidylpeptidase
3.4.14.5 Dipeptidylpeptidase IV
3.4.16.1 Serin-Typ Carboxypepetidase
3.4.17.1 Carboxypeptidase A
3.4.17.15 Carboxypeptidase A2
3.4.17.2 Carboxypeptidase b
3.4.17.4 Gly-X-Carboxypeptidase
3.4.21.48 Cerevisin
3.4.21.53 Endopeptidase Ia
3.4.21.61 Kexin
3.4.22.17 Calpain
3.4.23.18 Aspergillopepsin I
3.4.23.24 Candidapepsin
3.4.23.25 Saccharopepsin
3.4.23.5 Cathepsin D
3.4.24.15 Thimetoligopeptidase
3.4.24.37 Saccharolysin
3.4.24.57 O-Syaloglycoproteinendopeptidase
3.4.99.41 Mitochondriale Prozessierungs-Peptidase
3.4.99.45 Insulinase
3.4.99.46 Multicatalytischer Endopeptidasekomplex
3.5.1.1 Asparaginase
3.5.1.14 Aminoacylase
3.5.1.38 Glutamin-(Asparagin-)ase
3.5.1.4 Amidase
3.5.1.41 Chitindeacetylase
3.5.2.14 N-Methylhydantoinase (ATP-hydrolysierend)
3.5.2.2 Dihydropyrimidinase
3.5.2.3 Dihydroorotase
3.5.2.5 Allantoinase
3.5.3.1 Arginase
3.5.3.19 Ureidoglycolathydrolase
3.5.3.4 Allantoicase
3.5.4.10 IMP-Cyclohydrolase
3.5.4.12 dCMP-Deaminase
3.5.4.16 GTP-Cyclohydrolase I
3.5.4.19 Phosphoribosyl-AMP-Cyclohydrolase
3.5.4.25 GTP-Cyclohydrolase II
3.5.4.4 Adenosindeaminase
3.5.4.5 Cytidindeaminase
3.5.4.6 AMP-Deaminase
3.5.4.9 Methenyltetrahydrofolatcyclohydrolase
3.5.5.1 Nitrilase
3.6.1.1 anorganische Pyrophosphatase
3.6.1.11 Exopolyphosphatase
3.6.1.17 bis(5'-Nucleosyl)-tetraphosphatase (asymmetrisch)
3.6.1.23 dUTP-Pyrophosphatase
3.6.1.3 Adenosintriphosphatase
3.6.1.31 Phosphoribosyl-ATP-Pyrophosphatase
3.6.1.32 Myosin-ATPase
3.6.1.33 Dynein-ATPase
3.6.1.34 H+-transportierende ATP-Synthase
3.6.1.35 H+-transportierende ATPase
3.6.1.38 Ca2+-transportierende ATPase
3.6.1.42 Guanosindiphosphatase
3.6.1.5 Apyrase
3.6.1.9 Nucleotidpyrophosphatase
3.7.1.3 Kynureninase
4.1.1.1 Pyruvatdecarboxylase
4.1.1.15 Glutamatdecarboxylase
4.1.1.17 Ornithindecarboxylase
4.1.1.21 Phosphoribosylaminoimidazolcarboxylase
4.1.1.23 Orotidin-5'-phosphatdecarboxylase
4.1.1.33 Diphosphomevalonatdecarboxylase
4.1.1.37 Uroporphyrinogendecarboxylase
4.1.1.48 Indol-3-glycerolphosphatsynthase
4.1.1.49 Phosphoenolpyruvatcarboxykinase (ATP)
4.1.1.50 Adenosylmethionindecarboxylase
4.1.1.65 Phosphatidylserindecarboxylase
4.1.1.8 Oxalyl-CoA-Decarboxylase
4.1.2.13 Fructosebisphosphataldolase
4.1.2.15 2-Dehydro-3-desoxyphosphoheptonat-Aldolase
4.1.2.25 Dihydroneopterin-Aldolase
4.1.3.1 Isocitratlyase
4.1.3.12 2-Isopropylmalatsynthase
4.1.3.18 Acetolactatsynthase
4.1.3.2 Malatsynthase
4.1.3.27 Anthranilatsynthase
4.1.3.5 Hydroxymethylglutaryl-CoA-Synthase
4.1.3.7 Citrat(SI)synthase
4.1.99.3 Desoxyribodipyrimidinphotolyase
4.2.1.1 Carbonatdehydratase
4.2.1.10 3-Dehydrochinatdehydratase
4.2.1.11 Phosphopyruvathydratase
4.2.1.12 Phosphogluconatdehydratase
4.2.1.13 L-Serindehydratase
4.2.1.16 Threonindehydratase
4.2.1.17 Enoyl-CoA-Hydratase
4.2.1.19 Imidazolglycerolphosphatdehydratase
4.2.1.2 Fumarathydratase
4.2.1.20 Tryptophansynthase
4.2.1.22 Cystathionin-β-Synthase
4.2.1.24 Porphobilinogensynthase
4.2.1.3 Aconitathydratase
4.2.1.33 3-Isopropylmalatdehydratase
4.2.1.36 Homoaconitathydratase
4.2.1.51 Prephenatdehydratase
4.2.1.61 3-Hydroxypalmitoyl-[Acyl-Carrierprotein]- dehydratase
4.2.1.69 Cyanamidhydratase
4.2.1.75 Uroporphyrinogen-III-Synthase
4.2.1.9 Dihydroxysäuredehydratase
4.2.99.18 DNA-(apurinische oder apyrimidinische Sequenz)- lyase
4.2.99.2 Threoninsynthase
4.2.99.8 Cysteinsynthase
4.2.99.9 O-Succinylhomoserin-(Thiol)-lyase
4.3.1.8 Hydroxymethylbilansynthase
4.3.2.1 Argininosuccinatlyase
4.3.2.2 Adenylsuccinatlyase
4.4.1.1 Cystathionin-γ-lyase
4.4.1.11 Methionin-γ-lyase
4.4.1.17 Holocytochrom c-Synthase
4.4.1.5 Lactoylglutathionlyase
4.4.1.8 Cystathionin-β-lyase
4.6.1.1 Adenylatcyclase
4.6.1.3 3-Dehydrochinatsynthase
4.6.1.4 Chorismatsynthase
4.99.1.1 Ferrochelatase
5.1.3.1 Ribulosephosphat-3-epimerase
5.1.3.2 UDP-Glucose-4-epimerase
5.1.3.3 Aldose-1-epimerase
5.2.1.8 Peptidylprolylisomerase
5.3.1.1 Triosephosphatisomerase
5.3.1.24 Phosphoribosylanthranilatisomerase
5.3.1.4 L-Arabinoseisomerase
5.3.1.6 Ribose-5-phosphatepimerase
5.3.1.8 Mannose-6-phosphatisomerase
5.3.1.9 Glucose-6-phosphatisomerase
5.3.3.1 Steroid-Δ-isomerase
5.3.3.2lsopentenyldiphosphat-Δ-isomerase
5.3.4.1 Proteindisulfidisomerase
5.4.2.1 Phosphoglyceratmutase
5.4.2.2 Phosphoglucomutase
5.4.2.3 Phosphoacetylglucosaminmutase
5.4.2.4 Bisphosphoglyceratmutase
5.4.2.8 Phosphomannomutase
5.4.99.12 tRNA-Pseudouridinsynthase I
5.4.99.5 Chorismatmutase
5.4.99.7 Lanosterolsynthase
5.4.99.8 Cycloartenolsynthase
5.5.1.4 Myoinositol-1-phosphatsynthase
5.99.1.2 DNA-Topoisomerase
5.99.1.3 DNA-Topoisomerase (ATP-hydrolysierend)
6.1.1.1 Tyrosin-tRNA-Ligase
6.1.1.10 Methionin-tRNA-Ligase
6.1.1.11 Serin-tRNA-Ligase
6.1.1.12 Aspartat-tRNA-Ligase
6.1.1.14 Glycin-tRNA-Ligase
6.1.1.15 Prolin-tRNA-Ligase
6.1.1.16 Cystein-tRNA-Ligase
6.1.1.17 Glutamat-tRNA-Ligase
6.1.1.18 Glutamin-tRNA-Ligase
6.1.1.19 Arginin-tRNA-Ligase
6.1.1.2 Tryptophan-tRNA-Ligase
6.1.1.20 Phenylalanin-tRNA-Ligase
6.1.1.21 Histidin-tRNA-Ligase
6.1.1.22 Asparagin-tRNA-Ligase
6.1.1.3 Threonin-tRNA-Ligase
6.1.1.4 Leucin-tRNA-Ligase
6.1.1.5 Isoleucin-tRNA-Ligase
6.1.1.6 Lysin-tRNA-Ligase
6.1.1.7 Alanin-tRNA-Ligase
6.1.1.9 Valin-tRNA-Ligase
6.2.1.1 Acetat-CoA-Ligase
6.2.1.3 langkettige Fettsäure-CoA-Ligase
6.2.1.4 Succinat-CoA-Ligase (GTP-bildend)
6.3.1.2 Glutamat-Ammoniak-Ligase
6.3.2.1 Pantoat-β-Alaninligase
6.3.2.12 Dihydrofolatsynthase
6.3.2.17 Tetrahydrofolylpolyglutamatsynthase
6.3.2.19 Ubiquitin-Proteinligase
6.3.2.2 Glutamat-Cysteinligase
6.3.2.25 Tublin-Tyrosinligase
6.3.2.3 Glutathionsynthase
6.3.2.6 Phosphoribosylaminoimidazolsuccinocarboxyamidsynthase
6.3.3.1 Phosphoribosyl-Formylglycinamidincycloligase
6.3.3.2 5-Formyltetrahydrofolatcycloligase
6.3.3.3.3 Dethiobiotinsynthase
6.3.4.13 Phosphoribosylamin-Glycinligase
6.3.4.14 Biotincarboxylase
6.3.4.15 Biotin-[Acetyl-CoA-carboxylase]-ligase
6.3.4.16 Carbamoylphosphatsynthase (Ammoniak)
6.3.4.2 CTP-Synthase
6.3.4.3 Format-Tetrahydrofolatligase
6.3.4.4 Adenylosuccinatsynthase
6.3.4.5 Argininosuccinatsynthase
6.3.4.6 Harnstoffcarboxylase
6.3.5.2 GMP-Synthase (Glutamin-hydrolysierend)
6.3.5.3 Phosphoribosylformylglycinamidinsynthase
6.3.5.4 Asparaginsynthase (Glutamin-hydrolysierend)
6.3.5.5 Carbamoylphosphatsynthase (Glutamin-hydrolysierend)
6.4.1.1 Pyruvatcarboxylase
6.4.1.2 Acetyl-CoA-Carboxylase
6.5.1.1 DNA-Ligase (ATP)
6.5.1.4 RNA-3'-phosphatcyclase. In particular, one or more enzymes can be selected from the following list of proteins in the specified species as the target protein. E.coli enzymes with EC number 1.1.1.1 Alcohol dehydrogenase
1.1.1.100 3-oxoacyl- [acyl carrier protein] reductase
1.1.1.103 L-threonine-3-dehydrogenase
1.1.1.105 retinol dehydrogenase
1.1.1.122 D-threo-aldose-1-dehydrogenase
1.1.1.125 2-deoxy-D-gluconate-3-dehydrogenase
1.1.1.132 GDP-Mannose-6-dehydrogenase
1.1.1.133 DTDP-4-dehydrorhamnose reductase
1.1.1.14 L-Iditol-2-dehydrogenase
1.1.1.140 sorbitol-6-phosphate-2-dehydrogenase
1.1.1.145 3β-hydroxy-Δ5-steroid dehydrogenase
1.1.1.146 11β-hydroxysteroid dehydrogenase
1.1.1.157 3-hydroxybutyryl-CoA dehydrogenase
1.1.1.158 UDP-N-acetylmuramate dehydrogenase
1.1.1.159 7α-hydroxysteroid dehydrogenase
1.1.1.17 mannitol-1-phosphate-5-dehydrogenase
1.1.1.179 D-xylose-1-dehydrogenase (NADP +)
1.1.1.18 Myo-Inositol-2-dehydrogenase
1.1.1.188 Prostaglandin F synthase
1.1.1.195 Cinnamyl alcohol dehydrogenase
1.1.1.2 Alcohol Dehydrogenase (NADP +)
1.1.1.202 1,3-propanediol dehydrogenase
1.1.1.204 xanthine dehydrogenase
1.1.1.205 IMP dehydrogenase
1.1.1.21 aldehyde reductase
1.1.1.211 Long chain 3-hydroxyacyl-CoA dehydrogenase
1.1.1.218 morphine-6-dehydrogenase
1.1.1.22 UDP-glucose-6-dehydrogenase
1.1.1.23 histidine dehydrogenase
1.1.1.233 N-acylmannosamine-1-dehydrogenase
1.1.1.236 tropinone reductase
1.1.1.24 quinate-5-dehydrogenase
1.1.1.244 methanol dehydrogenase
1.1.1.25 Shikimate 5-dehydrogenase
1.1.1.27 L-lactate dehydrogenase
1.1.1.28 D-lactate dehydrogenase
1.1.1.29 Glycerate dehydrogenase
1.1.1.3 Homoserine Dehydrogenase
1.1.1.30 3-hydroxybutyrate dehydrogenase
1.1.1.31 3-Hydroxyisobutyrate dehydrogenase
1.1.1.35 3-Hydroxyacyl-CoA dehydrogenase
1.1.1.36 Acetoacetyl-CoA reductase
1.1.1.37 malate dehydrogenase
1.1.1.38 malate dehydrogenase (oxaloacetate decarboxylating)
1.1.1.40 Malate dehydrogenase (oxaloacetate decarboxylating) (NADP +)
1.1.1.42 isocitrate dehydrogenase (NADP +)
1.1.1.44 phosphogluconate dehydrogenase (decarboxylating)
1.1.1.47 glucose-1-dehydrogenase
1.1.1.48 galactose-1-dehydrogenase
1.1.1.49 glucose-6-phosphate-1-dehydrogenase
1.1.1.56 Ribitol-2-dehydrogenase
1.1.1.57 Fructuronate reductase
1.1.1.58 Tagaturonate reductase
1.1.1.6 Glycerol dehydrogenase
1.1.1.61 4-Hydroxybutyrate dehydrogenase
1.1.1.62 estradiol-17β-dehydrogenase
1.1.1.69 Gluconate 5-dehydrogenase
1.1.1.77 lactaldehyde reductase
1.1.1.8 Glycerol-3-phosphate dehydrogenase (NAD +)
1.1.1.81 hydroxypyruvate reductase
1.1.1.83 D-malate dehydrogenase (decarboxylating)
1.1.1.85 3-isopropylmalate dehydrogenase
1.1.1.86 Ketoleic acid reductoisomerase
1.1.1.91 Aryl alcohol dehydrogenase (NADP +)
1.1.1.93 Tartrate dehydrogenase
1.1.1.95 Phosphoglycerate dehydrogenase
1.1.2.3 L-lactate dehydrogenase (cytochrome)
1.1.2.4 D-lactate dehydrogenase (cytochrome)
1.1.3.13 alcohol oxidase
1.1.3.15 (S) -2-hydroxy acid oxidase
1.1.3.22 xanthine oxidase
1.1.3.4 glucose oxidase
1.1.99.1 Choline dehydrogenase
1.1.99.10 glucose dehydrogenase (acceptor)
1.1.99.17 glucose dehydrogenase (pyrroloquinoline quinone)
1.1.99.23 polyvinyl alcohol dehydrogenase (acceptor)
1.1.99.5 Glycerol-3-phosphate dehydrogenase
1.1.99.6 D-2-hydroxy acid dehydrogenase
1.1.99.8 alcohol dehydrogenase (acceptor)
1.10.2.2 Ubiquinol cytochrome c reductase
1.10.3.2 Laccase
1.10.3.3 L-ascorbate oxidase
1.11.1.12 Phospholipid hydroperoxide glutathione peroxidase
1.11.1.5 cytochrome c-peroxidase
1.11.1.6 Catalase
1.11.1.7 Peroxidase
1.11.1.9 Glutathione peroxidase
1.12.1.2 Hydrogen dehydrogenase
1.12.2.1 Cytochrome c3 hydrogenase
1.13.11.2 Catechol-2,3-dioxygenase
1.13.11.8 Protocatechuate 4,5-dioxygenase
1.13.12.4 Lactate-2-monooxygenase
1.14.12.10 Benzoate 1,2-dioxygenase
1.14.12.11 Toluene dioxygenase
1.14.12.12 Naphthalene-1,2-dioxygenase
1.14.12.3 Benzene-1,2-dioxygenase
1.14.13.2 4-Hydroxybenzoate-3-monooxygenase
1.14.13.20 2,4-dichlorophenol-6-monooxygenase
1.14.13.25 methane monooxygenase
1.14.13.3 4-Hydroxyphenylacetate-3-monooxygenase
1.14.13.7 Phenol-2-monooxygenase
1.14.14.3 Alkanal monooxygenase (linked to FMH)
1.15.1.1 Superoxide dismutase
1.16.1.1 Mercury (II) reductase
1.17.4.1 Ribonucleoside Diphosphate Reductase
1.17.4.2 Ribonucleoside triphosphate reductase
1.18.1.1 Rubredoxin NAD + reductase
1.18.1.2 Ferredoxin NADP + reductase
1.18.1.3 Ferredoxin NAD + reductase
1.18.1.4 Rubredoxin-NAD (P) + - reductase
1.18.99.1 hydrogenase
1.2.1.1 formaldehyde dehydrogenase (glutathione)
1.2.1.10 acetaldehyde dehydrogenase (acetylating)
1.2.1.11 Aspartate semialdehyde dehydrogenase
1.2.1.12 Glyceraldehyde-3-phosphate dehydrogenase (phosphorylating)
1.2.1.16 succinate semialdehyde dehydrogenase (NAD (P) +)
1.2.1.19 aminobutyraldehyde dehydrogenase
1.2.1.2 Format dehydrogenase
1.2.1.22 lactaldehyde dehydrogenase
1.2.1.3 Aldehyde Dehydrogenase (NAD +)
1.2.1.38 N-Acetyl-γ-glutamyl phosphate reductase
1.2.1.39 Phenylacetaldehyde dehydrogenase
1.2.1.40 3α, 7α, 12α-trihydroxycholestan-26-al
1.2.1.41 Glutamate 5-semialdehyde dehydrogenase
1.2.1.46 formaldehyde dehydrogenase
1.2.1.8 Betaine aldehyde dehydrogenase
1.2.1.9 Glyceraldehyde-3-phosphate dehydrogenase (NADP +)
1.2.2.2 Pyruvate dehydrogenase (cytochrome)
1.2.3.1 Aldehydroxidase
1.2.4.1 pyruvate dehydrogenase (lipoamide)
1.2.4.2 Oxoglutarate dehydrogenase (lipoamide)
1.2.7.1 Pyruvate Synthase
1.2.99.2 carbon monoxide dehydrogenase
1.2.99.4 formaldehyde dismutase
1.2.99.5 formylmethane furand dehydrogenase
1.3.1.12 Prephenate dehydrogenase
1.3.1.19 cis-1,2-dihydrobenzene-1,2-diol dehydrogenase
1.3.1.2 Dihydropyrimidine dehydrogenase (NADP +)
1.3.1.24 Biliverdin reductase
1.3.1.26 Dihydrodipicolinate reductase
1.3.1.28 2,3-Dihydro-2,3-dihydroxybenzoate dehydrogenase
1.3.1.29 cis-1,2-dihydro-1,2-dihydroxynaphthalene dehydrogenase
1.3.1.8 Acyl-CoA dehydrogenase (NADP +)
1.3.1.9 Enoyl- [Acyl Carrier Protein] reductase (NADH)
1.3.3.1 Dihydroorotate oxidase
1.3.3.3 Coproporphyrinogen Oxidase
1.3.3.4 Protoporphyrinogen oxidase
1.3.3.5 bilirubin oxidase
1.3.5.1 succinate dehydrogenase (ubiquinone)
1.3.99.1 succinate dehydrogenase
1.3.99.10 Isovaleryl-CoA dehydrogenase
1.3.99.13 Long chain acyl-CoA dehydrogenase
1.3.99.16
1.3.99.3 Acyl-CoA dehydrogenase
1.4.1.13 Glutamate Synthase (NADPH)
1.4.1.14 Glutamate Synthase (NADH)
1.4.1.4 Glutamate Dehydrogenase (NADP +)
1.4.3.16 L-aspartate oxidase
1.4.3.4 Amine Oxidase (Containing Flavin)
1.4.3.5 Pyridoxamine phosphate oxidase
1.4.3.6 Amine Oxidase (Containing Copper)
1.4.4.2 Glycine dehydrogenase (decarboxylating)
1.4.7.1 Glutamate Synthase (Ferredoxin)
1.4.99.1 D-amino acid dehydrogenase
1.5.1.12 1-pyrroline-5-carboxylate dehydrogenase
1.5.1.2 Pyrroline-5-carboxylate reductase
1.5.1.20 methylene tetrahydrofolate reductase (NADPH)
1.5.1.3 Dihydrofolate reductase
1.5.1.5 methylene tetrahydrofolate dehydrogenase (NADP +)
1.5.3.1 Sarcosine Oxidase
1.5.99.10
1.5.99.2 Dimethylglycine dehydrogenase
1.5.99.4 Nicotine dehydrogenase
1.5.99.7 Trimethylamine dehydrogenase
1.5.99.8 Proline dehydrogenase
1.6.1.1 NAD (P) + - transhydrogenase (b-specific)
1.6.2.4 NADPH ferrihemoprotein reductase
1.6.4.2 Glutathione reductase (NADPH)
1.6.4.5 Thioredoxin Reductase (NADPH)
1.6.5.3 NADH dehydrogenase (ubiquinone)
1.6.5.4 Monodehydroascorbate Reductase (NADH)
1.6.5.5 NADPH: quinone reductase
1.6.6.1 Nitrate reductase (NADH)
1.6.6.3 Nitrate reductase (NADPH)
1.6.6.4 Nitrite reductase (NAD (P) H)
1.6.6.8 GMP reductase
1.6.6.9 Trimethylamine N-oxide reductase
1.6.8.1 NPLD (P) H dehydrogenase (FMN)
1.6.99.1 NADPH dehydrogenase
1.6.99.2 NAD (P) H-dehydrogenase (quinone)
1.6.99.3 NADH dehydrogenase
1.7.99.4 nitrate reductase
1.7.99.5 5,10-methylene tetrahydrofolate reductase (FADH2)
1.8.1.2 Sulfite reductase (NADPH)
1.8.1.4 Dihydrolipoamide dehydrogenase
1.8.4.5 Methionine S-oxide reductase
1.8.4.6 Protein methionine S-oxide reductase
1.8.7.1 sulfite reductase (ferredoxin)
1.8.99.4
1.9.3.1 Cytochrome c oxidase
1.97.1.4 [Pyruvate format lyase] activating enzyme
1.97.4.1
2.1.1.107 Uroporphyrin-III c-methyltransferase
2.1.1.113 sequence-specific DNA methyltransferase (cytosine N4-specific)
2.1.1.13 5-methyltetrahydrofolate homocysteine S-methyltransferase
2.1.1.14 5-methyltetrahydropteroyl triglutamate homocysteine
2.1.1.17 Phosphatidylethanolamine N-methyltransferase
2.1.1.31 tRNA (guanine-N1 -) methyltransferase
2.1.1.35 tRNA (uracil-5 -) methyltransferase
2.1.1.37 DNA (cytosine 5 -) methyl transferase
2.1.1.45 thymidylate synthase
2.1.1.48 rmA (adenine N6 -) methyl transferase
2.1.1.63 methylated DNA-protein-cysteine-S-methyltransferase
2.1.1.64 3-Demethylubiquinone-9-3-O-methyltransferase
2.1.1.72 sequence-specific DNA methyltransferase (adenine-specific)
2.1.1.73 sequence-specific DNA methyltransferase (cytosine-specific)
2.1.1.77 Protein L-Isoaspartate (D-Aspartate) -O- Methyltransferase
2.1.1.79 Cyclopropane fatty acid acyl phospholipid synthase
2.1.1.80 Protein Glutamate-O-Methyltransferase
2.1.2.1 Glycine hydroxymethyl transferase
2.1.2.10 aminomethyl transferase
2.1.2.11 3-methyl-2-oxobutanoate hydroxymethyl transferase
2.1.2.2 Phosphoribosylglycinamide formyl transferase
2.1.2.3 Phosphoribosylaminoimidazole carboxamide formyl transferase
2.1.2.9 Methionyl tRNA formyl transferase
2.1.3.2 Aspartate carbamoyl transferase
2.1.3.3 Ornithine carbamoyl transferase
2.2.1.1 Transketolase
2.2.1.2 Transaldolase
2.3.1.1 Amino Acid N-Acetyl Transferase
2.3.1.117 2,3,4, 5-tetrahydropyridine-2-carboxylate-N-succinyl transferase
2.3.1.118 N-hydroxyarylamine-O-acetyltransferase
2.3.1.12 Dihydrolipoamide S acetyltransferase
2.3.1.128 Ribosome protein alanine N-acetyltransferase
2.3.1.129 Acyl- [Acyl Carrier Protein] -UDP-N-
2.3.1.15 Glycerol-3-phosphate-O-acyltransferase
2.3.1.16 Acetyl-CoA-C acyltransferase
2.3.1.18 Galactoside-O-acetyltransferase
2.3.1.28 ChlorAMPhenicol-O-acetyltransferase
2.3.1.29 Glycine-C-acetyltransferase
2.3.1.30 Serine O-acetyltransferase
2.3.1.37 5-aminolevulinate synthase
2.3.1.39 [Acyl Carrier Protein] -S-malonyltransferase
2.3.1.41 3-Oxoacyl [acyl carrier protein] synthase
2.3.1.46 Homoserine-O-succinyl transferase
2.3.1.47 8-amino-7-oxononanoate synthase
2.3.1.5 Arylamine-N-acetyltransferase
2.3.1.51 1-Acylglycerol-3-phosphate-O-acyltransferase
2.3.1.54 Format c-acetyltransferase
2.3.1.57 Diamine N-acetyltransferase
2.3.1.61 Dihydrolipoamide-S-succinyl transferase
2.3.1.74 Naringenin chalcone synthase
2.3.1.8 Phosphate acetyl transferase
2.3.1.9 Acetyl-CoA-C acetyltransferase
2.3.2.2 γ-glutamyl transferase
2.3.2.6 Leucyltransferase
2.4.1.1 Phosphorylase
2.4.1.11 Glycogen (starch) synthase
2.4.1.12 Cellulose Synthase (Forming UDP)
2.4.1.15 α, α-trehalose phosphate synthase (UDP-forming)
2.4.1.18 1,4-α-glucan branching enzyme
2.4.1.182 Lipid A disaccharide synthase
2.4.1.21 Starch synthase
2.4.1.24 1,4-α-glucan-6-α-glucosyltransferase
2.4.1.25 4-α-glucanotransferase
2.4.1.44 Lipopolysaccharide galactosyltransferase
2.4.1.58 Lipopolysaccharide Glucosyl Transferase I
2.4.1.7 Sucrosephsophorylase
2.4.1.83 Dolichylphosphate-β-D-mannosyltransferase
2.4.2.1 Purine Nucleoside Phosphorylase
2.4.2.10 OrotAT phosphoribosyl transferase
2.4.2.11 NicotinAT phosphoribosyl transferase
2.4.2.14 Amidophosphoribosyltransferase
2.4.2.17 ATP phosphoribosyl transferase
2.4.2.18 AnthranilAT phosphoribosyl transferase
2.4.2.19 Nicotinate nucleotide pyrophosphorylase (carboxylating)
2.4.2.2 Pyrimidine nucleoside phosphorylase
2.4.2.21 Nicotinate Nucleotide Dimethylbenzimidazole Phosphoribosyl
2.4.2.22 Xanthine phosphoribosyl transferase
2.4.2.28 5'-methylthioadenosine phosphorylase
2.4.2.29 Queuin tRNA ribosyltransferase
2.4.2.3 Uridine phosphorylase
2.4.2.4 Thymidine phosphorylase
2.4.2.7 Adenine phosphoribosyl transferase
2.4.2.8 Hypoxanthine phosphoribosyl transferase
2.4.2.9 uracil phosphoribosyl transferase
2.4.99.1 β-galactoside-α-2,6-sialyltransferase
2.5.1.1 Dimethylallyltrantransferase
2.5.1.10 Geranyltran transferase
2.5.1.15 Dihydropteroate synthase
2.5.1.16 Spermidine synthase
2.5.1.17 Cob (I) alamin adenosyl transferase
2.5.1.18 Glutathione transferase
2.5.1.19 3-Phosphoshikimate-1-carboxyvinyl transferase
2.5.1.22 sperm synthase
2.5.1.26 Alkylglycerone phosphate synthase
2.5.1.29 Farnesyltransferase
2.5.1.3 Thiamine Phosphate Pyrophosphorylase
2.5.1.31 Di-trans, poly-cis-decaprenylcistransferase
2.5.1.33 trans-pentaprenyltransferase
2.5.1.6 Methionine adenosyltransferase
2.5.1.7 UDP-N-Acetylglucosamine-1-Carboxyvinyltransferase
2.5.1.8 tRNA isopentenyl transferase
2.5.1.9 Riboflavin synthase
2.6.1.1 Aspartate Transaminase
2.6.1.11 Acetylornithine transaminase
2.6.1.13 Ornithine Oxo Acid Transaminase
2.6.1.14 Asparagine Oxo Acid Transaminase
2.6.1.16 glutamine-fructose-6-phosphate transaminase (isomerizing)
2.6.1.19 4-aminobutyrate transaminase
2.6.1.2 Alanine Transaminase
2.6.1.21 D-alanine transaminase
2.6.1.42 Branched Chain Amino Acid Transaminase
2.6.1.5 Tyrosine Transaminase
2.6.1.52 phosphoserine transaminase
2.6.1.57 Aromatic amino acid transaminase
2.6.1.62 Adenosylmethionine-8-amino-7-oxononanoate transaminase
2.6.1.64 Glutamine Phenylpyruvate Transaminase
2.6.1.66 valine pyruvate ransaminase
2.6.1.7 Kynurenine Oxoglutarate Transamininase
2.6.1.9 Histidinol phosphate transaminase
2.7.1.105 6-phosphofructo-2-kinase
2.7.1.107 diacylglycerol kinase
2.7.1.11 6-phosphofructokinase
2.7.1.116 [Isocitrate dehydrogenase (NADP +)] - kinase
2.7.1.12 Gluconokinase
2.7.1.15 Ribokinase
2.7.1.16 Ribulokinase
2.7.1.17 xylulokinase
2.7.1.19 Phosphoribulokinase
2.7.1.2 Glucokinase
2.7.1.21 Thymidine kinase
2.7.1.25 Adenylyl sulfate kinase
2.7.1.26 Riboflavin kinase
2.7.1.3 Ketohexokinase
2.7.1.30 glycerol kinase
2.7.1.33 Pantothenate kinase
2.7.1.35 pyridoxalkinase
2.7.1.37 protein kinase
2.7.1.39 Homoserine Kinase
2.7.1.4 Fructokinase
2.7.1.40 pyruvate kinase
2.7.1.45 2-dehydro-3-deoxygluconokinase
2.7.1.47 D-Ribulokinase
2.7.1.48 uridine kinase
2.7.1.49 hydroxymethylpyrimidine kinase
2.7.1.5 rhamnulokinase
2.7.1.50 Hydroxyethylthiazole kinase
2.7.1.52 fucokinase
2.7.1.53 L-xylulokinase
2.7.1.56 1-phosphofructokinase
2.7.1.6 Galactokinase
2.7.1.69 Protein-N (pai) -phosphohistidine-sugar-phosphotransferase
2.7.1.71 Shikimate kinase
2.7.1.73 inosine kinase
2.7.2.1 acetate kinase
2.7.2.11 Glutamate 5-kinase
2.7.2.2 carbamate kinase
2.7.2.3 Phosphoglycerate kinase
2.7.2.4 Aspartate Kinase
2.7.2.8 acetylglutamate kinase
2.7.3.9 Phosphoenolpyruvate protein phosphotransferase
2.7.4.1 polyphosphate kinase
2.7.4.14 Cytidylate kinase
2.7.4.16 Thiamine phosphate kinase
2.7.4.3 Adenylate kinase
2.7.4.6 Nucleoside diphosphate kinase
2.7.4.8 guanylate kinase
2.7.4.9 DTMP kinase
2.7.6.1 Ribosephosphate Pyrophosphokinase
2.7.6.3 2-Amino-4-hydroxy-6-hydroxymethyldihydropteridine pyro
2.7.6.5 GTP pyrophosphokinase
2.7.7.10 UTP hexose-1-phosphate uridylyl transferase
2.7.7.12 UDP-glucose-hexose-1-phosphate-pyridylyl transferase
2.7.7.13 Mannose 1-phosphate guanylyl transferase
2.7.7.19 Polynucleotide adenylyl transferase
2.7.7.2 FMN adenylyl transferase
2.7.7.22 Mannose-1-phosphate guanylyl transferase (GDP)
2.7.7.24 Glucose-1-phosphate-thymidylyl transferase
2.7.7.25 tRNA adenylyl transferase
2.7.7.27 Glucose-1-phosphate adenylyl transferase
2.7.7.38 3-deoxy-manno-octulosonate cytidylyl transferase
2.7.7.39 Glycerol-3-phosphate cytidylyl transferase
2.7.7.4 Sulfatadenylyl transferase
2.7.7.41 Phosphatidate cytidylyl transferase
2.7.7.42 [Glutamate ammonia ligase] adenylyl transferase
2.7.7.48 RNA-related RNA polymerase
2.7.7.49 RNA-related DNA polymerase
2.7.7.56 tRNA nucleotidyl transferase
2.7.7.59 [Protein PII] uridlylyl transferase
2.7.7.6 DNA-related RNA polymerase
2.7.7.7 DNA-related DNA polymerase
2.7.7.8 Polyribonucleotide Nucleotidyl Transferase
2.7.7.9 UTP-glucose-1-phosphate uridylyl transferase
2.7.8.13 Phospho-N-acetylmuramoyl pentapeptide transferase
2.7.8.15 UDP-N-acetylglucosamine dolichyl
2.7.8.20 Phosphatidylglycerol membrane oligosaccharide
2.7.8.5 CDP-diacylglycerol-glycerol-3-phosphate-3-phosphatidyl transferase
2.7.8.6 Undecaprenyl phosphate galactose phosphotransferase
2.7.8.8 CDP-diacylglycerol-serine-O-phosphatidyltransferase
2.7.9.2 Pyruvate water dikinase
2.8.1.1 Thiosulfate Sulfur Transferase
2.8.1.5 Thiosulfate dithiol sulfur transferase 2.8.3.5 3-Oxoacid-CoA transferase
2.8.3.6 3-oxoadipate-CoA transferase
2.8.3.9 Butyrate acetoacetate CoA transferase
2.9.1.1 L-Seryl-tRNA (Ser) Selenium Transferase
3.1.1.1 Carboxylesterase
3.1.1.10 tropinesterase
3.1.1.11 pectin esterase
3.1.1.2 aryl esterase
3.1.1.29 aminoacyl tBNA hydrolase
3.1.1.3 Triacylglycerol lipase
3.1.1.32 Phospholipase A1
3.1.1.45 carboxymethylene butene lipase
3.1.1.5 Lisophospholipase
3.1.1.61 protein glutamate methyl esterase
3.1.11.1 Exodesoxyribonuclease I
3.1.11.2 Exodesoxyribonuclease III
3.1.11.5 Exodesoxyribonuclease V
3.1.11.6 Exodesoxyribonuclease VII
3.1.13.1 Exoribonuclease II
3.1.2.1 Acetyl-CoA hydrolase
3.1.2.2 Palmitoyl-CoA hydrolase
3.1.2.6 Hydroxyacylglutathione hydrolase
3.1.21.1 Deoxyribonuclease I
3.1.21.2 Deoxyribonuclease IV (phage T4-induced)
3.1.21.3 Type I sequence specific deoxyribonuclease
3.1.22.4 Crossover junction endoribonuclease
3.1.25.1 Deoxyribonuclease (pyrimidine dimer)
3.1.26.3 Ribonuclease III
3.1.26.4 calf thymus ribonuclease H
3.1.26.5 Ribonuclease P
3.1.27.6 Enterobacter ribonuclease
3.1.3.1 alkaline phosphatase
3.1.3.10 glucose-1-phosphatase
3.1.3.11 Fructose bisphosphatase
3.1.3.12 Trehalose phosphatase
3.1.3.15 histidinol phosphatase
3.1.3.16 Phosphoprotein phosphatase
3.1.3.18 phosphoglycolate phosphatase
3.1.3.2 acid phosphatase
3.1.3.25 Myoinositol-1 (or 4) monophosphatase
3.1.3.27 Phosphatidylglycerophosphatase
3.1.3.3 Phosphoserine Phosphatase
3.1.3.41 4-nitrophenyl phosphatase
3.1.3.46 Fructose-2,6-bisphosphate-2-phosphatase
3.1.3.48 protein tyrosine phosphatase
3.1.3.5 5'-nucleotide
3.1.4.14 [Acyl Carrier Protein] phosphodiesterase
3.1.4.16 2 ', 3'-Cyclic Nucleotide 2'-Phosphodiesterase
3.1.4.17 3 ', 5'-cyclic nucleotide phosphodiesterase
3.1.4.46 glycerophosphodiester phosphodiesterase
3.1.5.1 dGTPase
3.1.6.1 aryl sulfatase
3.1.6.13 Iduronate-2-sulfatase
3.1.6.14 N-acetylglucosamine-6-sulfatase
3.1.6.2 Steryl sulfatase
3.1.6.4 N-acetylgalactosamine-6-sulfatase
3.1.6.8 Cerebroside sulfatase
3.1.7.2 guanosine-3 ', 5'-bis (diphosphate) 3'-pyrophosphatase
3.2.1.1 α-amylase
3.2.1.10 Oligo-1,6-glucosidase
3.2.1.135 Neopullulanase
3.2.1.14 chitinase
3.2.1.17 Lysozyme
3.2.1.20 α-glucosidase
3.2.1.21 β-glucosidase
3.2.1.22 α-galactosidase
3.2.1.23 β-galactosidase
3.2.1.24 α-mannosidase
3.2.1.28 α, α-trehalase
3.2.1.3 Glucan-1,4-α-glucosidase
3.2.1.31 β-glucuronidase
3.2.1.37 xylan-1,4-β-xylosidase
3.2.1.48 Sucrose-α-glucosidase
3.2.1.54 Cyclomaltodextrinase
3.2.1.68 Isoamylase
3.2.1.70 glucan-1,6-α-glucosidase
3.2.1.74 Glucan-1,4-β-glucosidase
3.2.1.8 Endo-1,4-β-xylanase
3.2.1.86 6-phospho-β-glucosidase
3.2.1.93 α, α-phosphotrehalase
3.2.2.20 DNA-3-methyladenine glycosidase I
3.2.2.21 DNA-3-methyladenine glycosidase II
3.2.2.23 Formamidopyrimidine DNA glycosidase
3.2.2.24 ADP-Ribosyl- [Nitrogen Reductase] hydrolase
3.2.2.4 AMP nucleosidase
3.3.2.1 Isochorismatase
3.3.2.3 Epoxy hydrolase
3.4.11.1 Leucyl aminopeptidase
3.4.11.10 bacterial leucyl aminopeptidase
3.4.11.15 Lysyl aminopeptidase
3.4.11.18 Methionyl aminopeptidase
3.4.11.2 Membrane alanyl aminopeptidase
3.4.11.5 Prolyl aminopeptidase
3.4.11.7 Glutamyl aminopeptidase
3.4.11.9 X-pro aminopeptidase
3.4.13.3 X-His dipeptidase
3.4.13.9 X-Pro Dipeptidase DCP
3.4.16.4 Serine type D-Ala, D-Ala carboxypeptidase
3.4.19.1 Acylaminoacyl peptidase
3.4.21.26 Prolyl oligopeptidase
3.4.21.53 Endopeptidase Ia
3.4.21.83 Oligopeptidase B
3.4.21.87 Omptin
3.4.21.92 Endopeptidase CLP
3.4.23.36 Signal peptidase II
3.4.24.13 IgA-specific metal endopeptidase
3.4.24.15 Thimetoligopeptidase
3.4.24.57 O-Syaloglycoprotein endopeptidase
3.4.99.36 lead peptidase
3.4.99.41 mitochondrial processing peptidase
3.4.99.44 Pitrilysin
3.4.99.45 insulinase
3.5.1.1 Asparaginase
3.5.1.10 Formyl tetrahydrofolate deformylase
3.5.1.11 Penicillin amidase
3.5.1.13 Aryl acylamidase
3.5.1.14 amino acylase
3.5.1.16 acetylornithine deacetylase
3.5.1.18 succinyl-diaminopimelate desuccinylase
3.5.1.2 Glutaminase
3.5.1.25 N-acetylglucosamine-6-phosphate deacetylase
3.5.1.26 N4- (β-N-acetylglucosaminyl) -L-asparaginase
3.5.1.27 N-Formylmethionylaminoacyl-tRNA deformylase
3.5.1.28 N-acetylmuramoyl-L-alanine amidase
3.5.1.32 Hippurate hydrolase
3.5.1.38 glutamine (asparagine) ase
3.5.1.59 N-carbamoyl sarcosine amidase
3.5.2.2 Dihydropyrimidinase
3.5.2.3 Dihydroorotase
3.5.2.5 Allantoinase
3.5.2.6 β-lactamase
3.5.3.1 Arginase
3.5.3.11 Agmatinase
3.5.3.19 Ureidoglycolate hydrolase
3.5.4.1 Cytosine disaminase
3.5.4.10 IMP cyclohydrolase
3.5.4.13 dCTP deaminase
3.5.4.16 GTP cyclohydrolase I
3.5.4.19 Phosphoribosyl AMP cyclohydrolase
3.5.4.2 Adenine Deaminase
3.5.4.25 GTP cyclohydrolase II
3.5.4.4 adenosine deaminase
3.5.4.5 Cytidine desaminase
3.5.4.9 Methenyl tetrahydrofolate cyclohydrolase
3.6.1.1 inorganic pyrophosphatase
3.6.1.11 Exopolyphosphatase
3.6.1.23 dUTP pyrophosphatase
3.6.1.26 CDP-diacylglycerol pyrophosphatase
3.6.1.31 Phosphoribosyl-ATP pyrophosphatase
3.6.1.32 Myosin ATPase
3.6.1.34 H + -transporting ATP synthase
3.6.1.35 H + transporting ATPase
3.6.1.36 H + / K + exchanging ATPase
3.6.1.37 Na + / K + exchanging ATPase
3.6.1.38 Ca2 + transporting ATPase
3.6.1.41 bis (5'-nucleosyl) tetraphosphatase (symmetrical)
3.6.1.45 UDP sugar diphosphatase
3.6.1.7 Acylphosphatase
3.7.1.8 2,6-Dioxo-6-phenylhexα-3-enoathydrolase
3.7.1.9 2-hydroxymuconate semialdehyde hydrolase
3.8.1.3 Haloacetate dalogenase
3.8.1.6 4-chlorobenzoate dehydrogenase
4.1.1.11 Aspartate 1 decarboxylase
4.1.1.15 glutamate decarboxylase
4.1.1.17 ornithine decarboxylase
4.1.1.18 Lysine decarboxylase
4.1.1.19 arginine decarboxylase
4.1.1.20 diaminopimelate decarboxylase
4.1.1.21 phosphoribosylaminoimidazole carboxylase
4.1.1.23 Orotidine 5'-phosphate decarboxylase
4.1.1.3 Oxaloacetate decarboxylase
4.1.1.31 Phosphoenolpyruvate carboxylase
4.1.1.37 uroporphyrinogen decarboxylase
4.1.1.41 methylmalonyl-CoA decarboxylase
4.1.1.47 tartronate semialdehyde synthase
4.1.1.48 Indole-3-glycerol phosphate synthase
4.1.1.49 Phosphoenolpyruvate Carboxykinase (ATP)
4.1.1.50 Adenosylmethionine decarboxylase
4.1.1.64 2,2-dialkylglycine decarboxylase (pyruvate)
4.1.1.65 Phosphatidylserine decarboxylase
4.1.1.71 2-oxoglutarate decarboxylase
4.1.1.73 Tartrate decarboxylase
4.1.1.8 Oxalyl-CoA decarboxylase
4.1.2.13 Fructose bisphosphate aldolase
4.1.2.14 2-Dehydro-3-deoxyphosphogluconate aldolase
4.1.2.15 2-Dehydro-3-deoxyphosphoheptonate aldolase
4.1.2.16 2-Dehydro-3-deoxyphosphooctonate aldolase
4.1.2.17 L-fuculose phosphate aldolase
4.1.2.19 rhamnulose-1-phosphate aldolase
4.1.2.25 Dihydroneopterinaldolase
4.1.2.4 Deoxyribosephosphataldolase
4.1.3. 1 isocitrate lyase
4.1.3.12 2-isopropylmalate synthase
4.1.3.16 4-Hydroxy-2-oxoglutarataldolase
4.1.3.18 Acetolactate synthase
4.1.3.2 Malate synthase
4.1.3.27 Anthranilate synthase
4.1.3.3 N-acetylneuraminate lyase
4.1.3.36 Naphthoate synthase
4.1.3.6 Citrate lyase
4.1.3.7 Citrate (SI) synthase
4.1.99.1 Tryptophanase
4.1.99.2 Tyrosine Phenollyase
4.1.99.3 deoxyribodipyrimidine photolyase
4.1.99.4 1-aminocyclopropane-1-carboxylate deaminase
4.2.1.1 carbonate dehydratase
4.2.1.10 3-dehydroquinate dehydratase
4.2.1.11 Phosphopyruvate hydratase
4.2.1.12 Phosphogluconate dehydratase
4.2.1.13 L-serine dehydratase
4.2.1.14 D-serine dehydratase
4.2.1.16 threonine dehydratase
4.2.1.17 Enoyl-CoA hydratase
4.2.1.19 Imidazole glycerol phosphate dehydratase
4.2.1.2 Fumarate hydratase
4.2.1.20 tryptophan synthase
4.2.1.24 Porphobilinogen synthase
4.2.1.3 Aconite hydratase
4.2.1.32 L (+) - tartrate dehydratase
4.2.1.33 3-isopropylmalate dehydratase
4.2.1.36 Homoaconitate hydratase
4.2.1.40 glucarate dehydratase
4.2.1.46 dTDP-glucose-4,6-dehydratase
4.2.1.51 Prephenate dehydratase
4.2.1.52 Dihydrodipicolinate synthase
4.2.1.60 3-hydroxydecanoyl- [acyl carrier protein] dehydratase
4.2.1.7 Altronate dehydratase
4.2.1.74 long chain enoyl-CoA hydratase
4.2.1.75 Uroporphyrinogen III synthase
4.2.1.8 Mannonate dehydratase
4.2.1.89 Carnitine dehydratase
4.2.1.9 Dihydroxy acid dehydratase
4.2.99.11 methylglyoxal synthase
4.2.99.18 DNA (apurinic or apyrimidine sequence) lyase
4.2.99.2 threonine synthase
4.2.99.8 cysteine synthase
4.2.99.9 O-succinyl homoserine (thiol) lyase
4.3.1.1 Aspartate ammonia lyase
4.3.1.7 Ethanolamine ammonia lyase
4.3.1.8 Hydroxymethylbilane Synthase
4.3.2.1 Argininosuccinate Lyase
4.3.2.2 Adenyl succinate lyase
4.3.99.1 cyanate lyase
4.4.1.1 Cystathionin-y-lyase
4.4.1.11 methionine-7-lyase
4.4.1.14 1-aminocyclopropane-1-carboxylate synthase
4.4.1.5 Lactoylglutathione lyase
4.4.1.8 Cystathionin-β-lyase
4.5.1.5 S-carboxymethylcysteine synthase
4.6.1.1 Adenylate cyclase
4.6.1.10 6-Pyruvoiltetrahydrobiopterin synthase
4.6.1.3 3-dehydroquinate synthase
4.6.1.4 Chorismate synthase
4.99.1.1 Ferrochelatase
5.1.1.1 Alanine racemase
5.1.1.11 Phenylalanine racemase (ATP hydrolyzing)
5.1.1.13 Aspartate racemase
5.1.1.3 Glutamate racemase
5.1.1.7 Diaminopimarate epimerase
5.1.2.2 Almond Racemase
5.1.3.1 Ribulosephosphate-3-epimerase
5.1.3.13 dTDP-4-dehydrorhamnose 3,5-epimerase
5.1.3.14 UDP-N-acetylglucosamine. 2-epimerase
5.1.3.2 UDP-Glucose.4-epimerase
5.1.3.3 Aldose.1-epimerase
5.1.3.4 L-Ribulosephosphate-4-epimerase
5.2.1.8 Peptidyl prolyl isomerase
5.3.1.1 Triose phosphate isomerase
5.3.1.10 Glucosamine-6-phosphate isomerase
5.3.1.12 Glucuronate isomerase
5.3.1.14 L-rhamnose isomerase
5.3.1.16 N- (5'-Phospho-D-ribosylformimino) -5-amino-1-
5.3.1.17 4-deoxy-L-threo-5-hexosulose uronate ketoisomerase
5.3.1.24 Phosphoribosylanthranilate isomerase
5.3.1.4 L-arabinose isomerase
5.3.1.5 xylose isomerase
5.3.1.6 Ribose-5-phosphate epimerase
5.3.1.8 Mannose-6-phosphate isomerase
5.3.1.9 Glucose-6-phosphate isomerase
5.3.3.1 Steroid Δ isomerase
5.3.3.10 5-carboxymethyl-2-hydroxymuconate Δ isomerase
5.3.3.2 Isopentenyl diphosphate Δ isomerase
5.3.3.8 Dodecenoyl-CoA-Δ-isomerase
5.3.4.1 Protein disulfide isomerase
5.4.2.1 Phosphoglycerate mutase
5.4.2.2 Phosphoglucomutase
5.4.2.6 β-phosphoglucomutase
5.4.2.7 Phosphopentomutase
5.4.2.8 Phosphomannomutase
5.4.2.9 Phosphoenolpyruvate mutase
5.4.3.8 Glutamate-1-semialdehyde-2,1-aminomutase
5.4.99.12 tRNA pseudouridine synthase I
5.4.99.2 Methylmalonyl-CoA mutase
5.4.99.5 Chorismate mutase
5.4.99.6 isochorismate synthase
5.5.1.1 Muconate cycloisomerase
5.5.1.2 3-carboxy-cis, cis-muconate cycloisomerase
5.5.1.7 Chloromuconate cycloisomerase
5.99.1.2 DNA topoisomerase
5.99.1.3 DNA topoisomerase (ATP hydrolyzing)
6.1.1.1 Tyrosine tRNA ligase
6.1.1.10 methionine tRNA ligase
6.1.1.11 Serine tPKA ligase
6.1.1.12 Aspartate tRNA ligase
6.1.1.14 glycine tRNA ligase
6.1.1.15 Proline tRNA ligase
6.1.1.16 cysteine tRNA ligase
6.1.1.17 glutamate tRNA ligase
6.1.1.18 glutamine tRNA ligase
6.1.1.19 arginine tHNA ligase
6.1.1.2 Tryptophan tRNA ligase
6.1.1.20 phenylalanine tRNA ligase
6.1.1.21 histidine tRNA ligase
6.1.1.22 Asparagine tRNA ligase
6.1.1.3 Threonine LENA ligase
6.1.1.4 Leucine tRNA ligase
6.1.1.5 isoleucine tRNA ligase
6.1.1.6 Lysine tRNA ligase
6.1.1.7 alanine tRNA ligase
6.1.1.9 valine tRNA ligase
6.2.1.1 Acetate-CoA ligase
6.2.1.12 4-Coumarate-CoA ligase
6.2.1.22 Citrate (Pro-3S) lyase ligase
6.2.1.26 O-Succinylbenzoate-CoA ligase
6.2.1.27 4-Hydroxybenzoate CoA ligase
6.2.1.3 long chain fatty acid CoA ligase
6.2.1.4 succinate CoA ligase (GTP-forming)
6.2.1.5 succinate CoA ligase (ADP-forming)
6.3.1.1 Aspartate ammonia ligase
6.3.1.2 Glutamate ammonia ligase
6.3.1.5 NAD + synthase
6.3.2.1 Pantoate (β-alanine ligase
6.3.2.12 Dihydrofolate synthase
6.3.2.13 UDP-N-acetylmuramoylalnyl-D-glutamate-2,6-diaminopimelate ligase
6.3.2.15 UDP-N-acetylmuramoylalanyl-D-glutamyl-2,6-
6.3.2.17 tetrahydrofolyl polyglutamate synthase
6.3.2.19 Ubiquitin protein ligase
6.3.2.2 Glutamate cysteine ligase
6.3.2.3 Glutathione synthase
6.3.2.4 D-Alanine-D-Alanine Ligase
6.3.2.6 Phosphoribosylaminoimidazole succinocarboxyamide synthase
6.3.2.8 UDP-N-acetylmuramate alanine ligase
6.3.2.9 UDP-N-acetylmuramoylalanine-D-glutamate ligase
6.3.3.1 Phosphoribosyl-Formylglycinamidinecycloligase
6.3.3.2 5-Formyltetrahydrofolatecycloligase
6.3.3.3 Dethiobiotin synthase
6.3.4.13 Phosphoribosylamine glycine ligase
6.3.4.14 Biotin carboxylase
6.3.4.15 biotin [acetyl-CoA carboxylase] ligase
6.3.4.2 CTP synthase
6.3.4.4 Adenylosuccinate Synthase
6.3.4.5 argininosuccinate synthase
6.3.4.6 urea carboxylase
6.3.5.1 NAD + synthase (glutamine hydrolyzing)
6.3.5.2 GMP synthase (glutamine hydrolyzing)
6.3.5.3 Phosphoribosylformylglycinamidine synthase
6.3.5.4 asparagine synthase (glutamine hydrolyzing)
6.3.5.5 Carbamoyl phosphate synthase (glutamine hydrolyzing)
6.4.1.2 Acetyl-CoA carboxylase
6.4.1.3 Propionyl-CoA carboxylase
6.5.1.2 DNA ligase (NAD +). Enzymes from Corynebacteriurn glutamicum with EC number 1.1.1.1 Alcohol dehydrogenase
1.1.1.100 3-oxoacyl- [acyl carrier protein] reductase
1.1.1.105 retinol dehydrogenase
1.1.1.133 dTDP-4-dehydrorhamnose reductase
1.1.1.158 UDP-N-acetylmuramate dehydrogenase
1.1.1.169 2-dehydropantoate-2-reductase
1.1.1.17 mannitol-1-phosphate-5-dehydrogenase
1.1.1.18 Myoinositol-2-dehydrogenase
1.1.1.195 Cinnamyl alcohol dehydrogenase
1.1.1.2 Alcohol Dehydrogenase (NADP +)
1.1.1.205 IMP dehydrogenase
1.1.1.21 aldehyde reductase
1.1.1.218 morphine-6-dehydrogenase
1.1.1.22 UDP-glucose-6-dehydrogenase
1.1.1.23 histidine dehydrogenase
1.1.1.25 Shikimate 5-dehydrogenase
1.1.1.27 L-lactate dehydrogenase
1.1.1.28 D-lactate dehydrogenase
1.1.1.29 Glycerate dehydrogenase
1.1.1.3 Homoserine Dehydrogenase
1.1.1.36 Acetoacetyl-CoA reductase
1.1.1.37 malate dehydrogenase
1.1.1.38 Malate dehydrogenase (oxaloacetate decarboxylating)
1.1.1.4 (R, R) -butanediol dehydrogenase
1.1.1.42 isocitrate dehydrogenase (NADP +)
1.1.1.44 phosphogluconate dehydrogenase (decarboxylating)
1.1.1.47 glucose-1-dehydrogenase
1.1.1.49 glucose-6-phosphate 1-dehydrogenase
1.1.1.69 Gluconate 5-dehydrogenase
1.1.1.8 Glycerol-3-phosphate dehydrogenase (NAD +)
1.1.1.85 3-isopropylmalate dehydrogenase
1.1.1.86 keto acid reductoisomerase
1.1.1.90 Aryl alcohol dehydrogenase
1.1.1.91 Aryl alcohol dehydrogenase (NADP +)
1.1.1.95 Phosphoglycerate dehydrogenase
1.1.2.3 L-lactate dehydrogenase (cytochrome)
1.1.2.4 D-lactate dehydrogenase (cytochrome)
1.1.3.15 (S) -2-hydroxy acid oxidase
1.1.99.5 Glycerol-3-phosphate dehydrogenase
1.10.3.2 Laccase
1.11.1.12 Phospholipid hydroperoxide glutathione peroxidase
1.11.1.6 Catalase
1.11.1.9 Glutathione peroxidase
1.13.11.1 Catechol-1,2-dioxygenase
1.13.11.27 4-hydroxyphenylpyruvate dioxygenase
1.13.11.32 2-nitropropane dioxygenase
1.13.11.37 Hydroxyquinol 1,2-dioxygenase
1.14.12.3 Benzene-1,2-dioxygenase
1.14.13.1 Salicylate-1-monooxygenase
1.14.13.2 4-Hydroxybenzoate 3-monooxygenase
1.14.13.20 2,4-dichlorophenol-6-monooxygenase
1.14.13.7 Phenol-2-monooxygenase
1.14.13.8 Dimethylaniline monooxygenase (N-oxide-forming)
1.14.14.3 Alkanal monooxygenase (linked to FMH)
1.14.99.3 hemoxygenase (decyclizing)
1.15.1.1 Superoxide dismutase 1.16.1.1 Mercury (II) reductase
1.17.4.1 Ribonucleoside Diphosphate Reductase
1.18.1.2 Ferredoxin NADP + reductase
1.18.1.4 Rubredoxin-NAD (P) + - reductase
1.2.1.1 formaldehyde dehydrogenase (glutathione)
1.2.1.11 Aspartate semialdehyde dehydrogenase
1.2.1.12 Glyceraldehyde-3-phosphate dehydrogenase (phosphorylating)
1.2.1.13 Glyceraldehyde-3-phosphate dehydrogenase (NADP +) (phosphorylating)
1.2.1.16 succinate semialdehyde dehydrogenase (NAD (P) +)
1.2.1.2 Format dehydrogenase
1.2.1.22 lactaldehyde dehydrogenase
1.2.1.27 methylmalonate semialdehyde dehydrogenase (acylating)
1.2.1.3 Aldehyde Dehydrogenase (NAD +)
1.2.1.38 N-Acetyl-γy-glutamylphosphate reductase
1.2.1.39 Phenylacetaldehyde dehydrogenase
1.2.1.41 Glutamate 5-semialdehyde dehydrogenase
1.2.1.8 Betaine aldehyde dehydrogenase
1.2.2.2 Pyruvate dehydrogenase (cytochrome)
1.2.3.3 Pyruvate Oxidase
1.2.4.1 pyruvate dehydrogenase (lipoamide)
1.2.4.2 Oxoglutarate dehydrogenase (lipoamide)
1.3.1.12 Prephenate dehydrogenase
1.3.1.26 Dihydrodipicolinate reductase
1.3.1.28 2,3-Dihydro-2,3-dihydroxybenzoate hydrogenase
1.3.1.32 Maleyl acetate reductase
1.3.1.42 12-Oxophytodienoate reductase
1.3.1.45 2'-Hydroxyisoflavone reductase
1.3.3.1 Dihydroorotate oxidase
1.3.3.3 Coproporphyrinogen Oxidase
1.3.3.4 Protoporphyrinogen oxidase
1.3.3.5 bilirubin oxidase
1.3.5.1 succinate dehydrogenase (ubiquinone)
1.3.99.1 succinate dehydrogenase
1.3.99.3 Acyl-CoA dehydrogenase
1.3.99.4 3-Oxosteroid-1-dehydrogenase
1.3.99.5 3-Oxo-5α-steroid-4-dehydrogenase
1.4.1.13 Glutamate Synthase (NADPH)
1.4.1.14 Glutamate Synthase (NADH)
1.4.1.16 Diaminopimelate dehydrogenase
1.4.1.4 Glutamate Dehydrogenase (NADP +)
1.4.99.1 D-amino acid dehydrogenase
1.5.1.12 1-pyrroline-5-carboxylate dehydrogenase
1.5.1.15 Methylenetetrahydrofolate dehydrogenase (NAD +)
1.5.1.2 Pyrroline-5-carboxylate reductase
1.5.1.20 methylenetetrahydrofolate reductase (NADPH)
1.5.1.3 Dihydrofolate reductase
1.5.1.5 methylene tetrahydrofolate dehydrogenase (NADP +)
1.5.3.1 Sarcosine Oxidase
1.5.99.8 Proline dehydrogenase
1.6.4.2 Glutathione reductase (NADPH)
1.6.4.5 Thioredoxin Reductase (NADPH)
1.6.5.3 NADH dehydrogenase (ubiquinone)
1.6.5.5 NADPH: quinone reductase
1.6.8.2 NADPH dehydrogenase (flavin)
1.6.99.1 NADPH dehydrogenase
1.7.7.1 Ferredoxin nitrite reductase
1.7.99.4 nitrate reductase
1.7.99.5 5,10-methylene tetrahydrofolate reductase (FADH2)
1.8.1.4 Dihydrolipoamide dehydrogenase
1.8.4.6 Protein methionine S-oxide reductase
1.8.99.4
1.9.3.1 Cytochrome c oxidase
2.1.1.107 Uroporphyrin-III C-methyltransferase
2.1.1.13 5-methyltetrahydrofolate homocysteine S-methyltransferase
2.1.1.14 5-methyltetrahydropteroyl triglutamate homocysteine
2.1.1.31 tRNA- (guanine-N1-) methyltransferase
2.1.1.37 DNA (cytosine 5 -) methyltransferase
2.1.1.41 24-sterol c-methyl transferase
2.1.1.45 thymidylate synthase
2.1.1.48 rRNA- (adenine-N6-) methyltransferase
2.1.1.63 methylated-DNA-protein-cysteine-S-methyltransferase
2.1.1.72 sequence-specific DNA methyltransferase (adenine-specific)
2.1.1.73 sequence-specific DNA methyltransferase (cytosine-specific)
2.1.1.79 Cyclopropane fatty acid acyl phospholipid synthase
2.1.2.1 Glycine hydroxymethyl transferase
2.1.2.11 3-methyl-2-oxobutanoate hydroxymethyl transferase
2.1.2.2 Phosphoribosylglycinamide f-formyl transferase
2.1.2.3 Phosphoribosylaminoimidazole carboxamide formyl transferase
2.1.2.9 Methionyl tRNA formyl transferase
2.1.3.2 Aspartate carbamoyl transferase
2.1.3.3 ornithine carbamoyl transferase
2.2.1.1 Transketolase
2.2.1.2 Transaldolase
2.3.1.128 Ribosomal protein alanine N-acetyltransferase
2.3.1.18 Galactoside-O-acetyltransferase
2.3.1.28 Chloramphenicol-O-acetyltransferase
2.3.1.30 Serine O-acetyltransferase
2.3.1.31 Homoserine-O-acetyltransferase
2.3.1.35 Glutamate-N-acetyltransferase
2.3.1.51 1-Acylglycerol-3-phosphate-O-acyltransferase
2.3.1.61 Dihydrolipoamide-S-succinyl transferase
2.3.1.8 Phosphate acetyl transferase
2.3.1.85 fatty acid synthase
2.3.2.2 γ-glutamyl transferase
2.3.2.5 Glutaminyl peptide cyclotransferase
2.4.1.1 Phosphorylase
2.4.1.11 Glycogen (starch) synthase
2.4.1.15 α, α-trehalose phosphate synthase (UDP-forming)
2.4.1.17 Glucuronosyltransferase
2.4.1.18 1,4-α-glucan branching enzyme
2.4.1.19 Cyclomaltodextrin glucanotransferase
2.4.1.24 1,4-α-glucan-6-α-glucosyltransferase
2.4.1.25 4-α-glucanotransferase
2.4.1.52 Poly (glycerol phosphate) -α-glucosyltransferase
2.4.1.83 Dolichylphosphate-β-D-mannosyltransferase
2.4.2.10 Orotate phosphoribosyl transferase
2.4.2.14 Amidophosphoribosyltransferase
2.4.2.17 ATP phosphoribosyl transferase
2.4.2.18 Anthranilate phosphoribosyl transferase
2.4.2.19 Nicotinate nucleotide pyrophosphorylase (carboxylating)
2.4.2.29 Queuin tRNA ribosyltransferase
2.4.2.7 Adenine phosphoribosyl transferase
2.4.2.8 Hypoxanthine phosphoribosyl transferase
2.4.2.9 uracil phosphoribosyl transferase
2.5.1.1 Dimethylallyltrantransferase
2.5.1.10 Geranyltran transferase
2.5.1.15 Dihydropteroate synthase
2.5.1.16 Spermidine synthase
2.5.1.19 3-Phosphoshikimate-1-carboxyvinyl transferase
2.5.1.26 Alkylglycerone phosphate synthase
2.5.1.29 Farnesyltransferase
2.5.1.3 Thiamine phosphate pyrophosphorylase
2.5.1.31 Di-trans, poly-cis-decaprenylcistransferase
2.5.1.6 Methionine adenosyltransferase
2.5.1.7 UDP-N-Acetylglucosamine-1-carboxyvinyltransferase
2.5.1.8 tRNA isopentenyl transferase
2.5.1.9 Riboflavin synthase
2.6.1.1 Aspartate Transaminase
2.6.1.11 Acetylornithine transaminase
2.6.1.13 Ornithine Oxo Acid Transaminase
2.6.1.14 Asparagine Oxo Acid Transaminase
2.6.1.16 glutamine-fructose-6-phosphate transaminase (isomerizing)
2.6.1.18 β-alanine pyruvate transaminase
2.6.1.19 4-aminobutyrate transaminase
2.6.1.21 D-alanine transaminase
2.6.1.52 phosphoserine transaminase
2.6.1.62 Adenosylmethionine-8-amino-7-oxononanoate transaminase
2.6.1.64 Glutamine phenyl pyruvate transaminase
2.6.1.7 Kynurenine oxoglutarate transaminase
2.6.1.9 Histidinol phosphate transaminase
2.7.1.11 6-phosphofructokinase
2.7.1.12 Gluconokinase
2.7.1.15 Ribokinase
2.7.1.17 xylulokinase
2.7.1.25 Adenylyl sulfate kinase
2.7.1.26 Riboflavin kinase
2.7.1.30 glycerol kinase
2.7.1.33 Pantothenate kinase
2.7.1.35 pyridoxalkinase
2.7.1.37 protein kinase
2.7.1.39 Homoserine Kinase
2.7.1.4 Fructokinase
2.7.1.40 pyruvate kinase
2.7.1.49 hydroxymethylpyrimidine kinase
2.7.1.56 1-phosphofructokinase
2.7.1.69 Protein-N (pai) -phosphohistidine-sugar-phosphotransferase
2.7.1.71 Shikimate kinase
2.7.2.1 acetate kinase
2.7.2.11 Glutamate 5-kinase
2.7.2.3 Phosphoglycerate kinase
2.7.2.4 Aspartate Kinase
2.7.2.8 acetylglutamate kinase
2.7.3.9 Phosphoenolpyruvate protein phosphotransferase
2.7.4.14 Cytidylate kinase
2.7.4.16 Thiamine phosphate kinase
2.7.4.3 Adenylate kinase
2.7.4.6 Nucleoside diphosphate kinase
2.7.4.8 guanylate kinase
2.7.6.1 Ribosephosphate pyrophosphokinase
2.7.6.3 2-Amino-4-hydroxy-6-hydroxymethyldihydropteridine pyro
2.7.6.5 GTP pyrophosphokinase
2.7.7.13 Mannose 1-phosphate guanylyl transferase
2.7.7.2 FMN adenylyl transferase
2.7.7.24 Glucose-1-phosphate-thymidylyl transferase
2.7.7.25 tRNA adenylyl transferase
2.7.7.27 Glucose-1-phosphate adenylyl transferase
2.7.7.4 Sulfate adenylyl transferase
2.7.7.41 Phosphatidate cytidylyl transferase
2.7.7.42 [Glutamate ammonia ligase] adenylyl transferase
2.7.7.56 tRNA nucleotidyl transferase
2.7.7.59 [Protein PII] -Uridylyltransferase
2.7.7.6 DNA-related RNA polymerase
2.7.7.7 DNA-related DNA polymerase
2.7.7.8 Polyribonucleotide Nucleotidyl Transferase
2.7.7.9 UTP-glucose-1-phosphate uridylyl transferase
2.7.8.13 Phospho-N-acetylmuramoyl pentapeptide transferase
2.7.8.5 CDP-diacylglycerol-glycerol-3-phosphate-3-phosphatidyltransferase
2.7.8.6 Undecaprenyl phosphate galactose phosphotransferase
2.7.8.7 Holo [acyl carrier protein] synthase
2.7.9.2 Pyruvate water dikinase
2.8.1.1 Thiosulfate Sulfur Transferase
3.1.1.1 Carboxylesterase
3.1.1.29 aminoacyl tRNA hydrolase
3.1.1.3 triacylglycerol lipase
3.1.1.59 youth hormone esterase
3.1.1.8 cholinesterase
3.1.11.2 Exodesoxyribonuclease III
3.1.11.6 Exodesoxyribonuclease VII
3.1.13.1 Exoribonuclease II
3.1.2.1 Acetyl-CoA hydrolase
3.1.2.2 Palmitoyl-CoA hydrolase
3.1.2.6 Hydroxyacylglutathione hydrolase
3.1.21.3 Type I sequence-specific deoxyribonuclease
3.1.21.5 Type III sequence-specific deoxyribonuclease
3.1.22.4 Crossover junction endoribonuclease
3.1.26.3 Ribonuclease III
3.1.26.4 calf thymus ribonuclease H
3.1.26.5 Ribonuclease P
3.1.3.1 alkaline phosphatase
3.1.3.12 Trehalose phosphatase
3.1.3.15 histidinol phosphatase
3.1.3.16 Phosphoprotein phosphatase
3.1.3.18 phosphoglycolate phosphatase
3.1.3.2 acid phosphatase
3.1.3.25 Myoinositol-1 (or 4) monophosphatase
3.1.3.3 Phosphoserine Phosphatase
3.1.3.48 protein tyrosine phosphatase
3.1.3.5 5'-nucleotide
3.1.4.46 glycerophosphodiester phosphodiesterase
3.1.5.1 dGTPase
3.1.6.1 aryl sulfatase
3.1.7.2 guanosine-3 ', 5'-bis (diphosphate) 3'-pyrophosphatase
3.2.1.1 α-amylase
3.2.1.10 Oligo-1,6-glucosidase
3.2.1.135 Neopullulanase
3.2.1.18 Exo-α-sialidase
3.2.1.20 α-glucosidase
3.2.1.21 β-glucosidase
3.2.1.26 β-fructofuranosidase
3.2.1.37 xylan-1,4-β-xylosidase
3.2.1.41 α-dextrin-endo-1,6-α-glucosidase
3.2.1.48 Sucrose-α-glucosidase
3.2.1.54 Cyclomaltodextrinase
3.2.1.58 Glucan-1,3-β-glucosidase
3.2.1.68 Isoamylase
3.2.1.7 inulinase
3.2.1.74 Glucan-1,4-β-glucosidase
3.2.1.86 6-phospho-β-glucosidase
3.2.1.93 α, α-phosphotrehalase
3.2.2.20 DNA-3-methyladenine glycosidaseI
3.2.2.23 Formamidopyrimidine DNA glycosidase
3.2.2.4 AMP nucleosidase
3.3.1.1 Adenosyl homocysteinase
3.3.2.3 Epoxy hydrolase
3.4.11.15 Lysyl aminopeptidase
3.4.11.18 Methionyl aminopeptidase
3.4.11.2 Membrane alanyl aminopeptidase
3.4.11.5 Prolyl aminopeptidase
3.4.13.9 X-Pro Dipeptidase DCP
3.4.16.4 Serine type D-Ala, D-Ala carboxypeptidase
3.4.17.4 Gly-X carboxypeptidase
3.4.21.1 Chymotrypsin
3.4.21.26 Prolyl oligopeptidase
3.4.21.4 trypsin
3.4.21.62 Subtilisin
3.4.21.66 Thermitase
3.4.21.83 Oligopeptidase B
3.4.23.36 Signal peptidase II
3.4.24.11 Neprilysin
3.4.24.37 Saccharolysin
3.4.24.57 O-Syaloglycoprotein endopeptidase
3.4.99.36 lead peptidase
3.5.1.1 Asparaginase
3.5.1.10 Formyl tetrahydrofolate deformylase
3.5.1.13 Aryl acylamidase
3.5.1.14 amino acylase
3.5.1.16 acetylornithine deacetylase
3.5.1.18 succinyl-diaminopimelate desuccinylase
3.5.1.2 Glutaminase
3.5.1.27 N-Formylmethionylaminoacyl-tRNA deformylase
3.5.1.28 N-acetylmuramoyl-L-alanine amidase
3.5.1.32 Hippurate hydrolase
3.5.1.38 glutamine (asparagine) ase
3.5.1.4 Amidase
3.5.1.5 urease
3.5.2.12 6-aminohexanoate cyclic dimer hydrolase
3.5.2.3 Dihydroorotase
3.5.4.10 IMP cyclohydrolase
3.5.4.13 dCTP deaminase
3.5.4.16 GTP cyclohydrolase I
3.5.4.19 Phosphoribosyl AMP cyclohydrolase
3.5.4.25 GTP cyclohydrolase II
3.5.4.9 Methenyl tetrahydrofolate cyclohydrolase
3.6.1.1 inorganic pyrophosphatase
3.6.1.11 Exopolyphosphatase
3.6.1.23 dUTP pyrophosphatase
3.6.1.3 Adenosine triphosphatase
3.6.1.31 Phosphoribosyl-ATP pyrophosphatase
3.6.1.34 H + -transporting ATP synthase
3.6.1.35 H + transporting ATPase
3.6.1.36 H + / K + -exchanging ATPase
3.6.1.38 Ca2 + transporting ATPase
3.6.1.7 Acylphosphatase
4.1.1.11 Aspartate 1-decarboxylase
4.1.1.17 ornithine decarboxylase
4.1.1.20 diaminopimelate decarboxylase
4.1.1.21 phosphoribosylaminoimidazole carboxylase
4.1.1.23 Orotidine 5'-phosphate decarboxylase
4.1.1.31 Phosphoenolpyruvate carboxylase
4.1.1.32 Phosphoenolpyruvate Carboxykinase (GTP)
4.1.1.37 uroporphyrinogen decarboxylase
4.1.1.41 methylmalonyl-CoA decarboxylase
4.1.1.44 4-Carboxymuconolactone decarboxylase
4.1.1.48 Indole-3-glycerol phosphate synthase
4.1.2.13 Fructose bisphosphate aldolase
4.1.2.15 2-Dehydro-3-deoxyphosphoheptonate aldolase
4.1.2.25 Dihydroneopterinaldolase
4.1.2.4 Deoxyribosephosphataldolase
4.1.3.1 Isocitrate lyase
4.1.3.12 2-isopropylmalate synthase
4.1.3.18 Acetolactate synthase
4.1.3.2 Malate synthase
4.1.3.27 Anthranilate synthase
4.1.3.36 Naphthoate synthase
4.1.3.6 Citrate lyase
4.1.3.7 Citrate (SI) synthase
4.1.99.3 deoxyribodipyrimidine photolyase
4.2.1.1 carbonate dehydratase
4.2.1.10 3-dehydroquinate dehydratase
4.2.1.11 Phosphopyruvate hydratase
4.2.1.13 L-serine dehydratase
4.2.1.16 threonine dehydratase
4.2.1.17 Enoyl-CoA hydratase
4.2.1.19 imidazole glycerol phosphate dehydratase
4.2.1.2 Fumarate hydratase
4.2.1.20 tryptophan synthase
4.2.1.24 Porphobilinogen synthase
4.2.1.3 Aconite hydratase
4.2.1.33 3-isopropylmalate dehydratase
4.2.1.46 dTDP-glucose-4,6-dehydratase
4.2.1.51 Prephenate dehydratase
4.2.1.52 Dihydrodipicolinate synthase
4.2.1.70 pseudouridylate synthase
4.2.1.9 Dihydroxy acid dehydratase
4.2.1.96 tetrahydrobiopterine dehydratase
4.2.99.18 DNA (apurinic or apyrimidine sequence) lyase
4.2.99.2 threonine synthase
4.2.99.8 cysteine synthase
4.2.99.9 O-succinyl homoserine (thiol) lyase
4.3.1.1 Aspartate Ammonia Lyase
4.3.1.12 Ornithine cyclodeaminase
4.3.1.8 Hydroxymethylbilane Synthase
4.3.2.1 Argininosuccinate Lyase
4.3.2.2 Adenyl succinate lyase
4.4.1.1 Cystathionin γ-lyase
4.4.1.17 Holocytochrome c synthase
4.4.1.5 Lactoylglutathione lyase
4.4.1.8 Cystathionin-β-lyase
4.5.1.5 S-carboxymethylcysteine synthase
4.6.1.1 Adenylate cyclase
4.6.1.3 3-dehydroquinate synthase
4.6.1.4 Chorismate synthase
4.99.1.1 Ferrochelatase
5.1.1.1 Alanine racemase
5.1.1.3 Glutamate racemase
5.1.1.7 Diaminopimarate epimerase
5.1.3.1 Ribulosephosphate-3-epimerase
5.1.3.2 UDP-Glucose-4-epimerase
5.2.1.8 Peptidyl prolyl isomerase
5.3.1.1 Triose phosphate isomerase
5.3.1.12 Glucuronate isomerase
5.3.1.16 N- (5'-Phospho-D-ribosylformimino) -5-amino-1-
5.3.1.24 Phosphoribosylanthranilate isomerase
5.3.1.6 Ribose-5-phosphate epimerase
5.3.1.8 Mannose-6-phosphate isomerase
5.3.1.9 Glucose-6-phosphate isomerase
5.3.3.2 Isopentenyl diphosphate Δ isomerase
5.4.2.1 Phosphoglycerate mutase
5.4.2.2 Phosphoglucomutase
5.4.2.8 Phosphomannomutase
5.4.2.9 Phosphoenolpyruvate mutase
5.4.3.8 Glutamate-1-semialdehyde-2,1-aminomutase
5.4.99.12 tRNA pseudouridine synthase I
5.4.99.2 Methylmalonyl-CoA mutase
5.4.99.6 isochorismate synthase
5.5.1.2 3-carboxy-cis, cis-muconate cycloisomerase
5.99.1.2 DNA topoisomerase
5.99.1.3 DNA topoisomerase (ATP hydrolyzing)
6.1.1.1 Tyrosine tRNA ligase
6.1.1.10 methionine tRNA ligase
6.1.1.11 Serine tRNA ligase
6.1.1.12 Aspartate tRNA ligase
6.1.1.14 glycine tRNA ligase
6.1.1.15 Proline tRNA ligase
6.1.1.16 cysteine tRNA ligase
6.1.1.17 glutamate tRNA ligase
6.1.1.19 arginine tRNA ligase
6.1.1.2 Tryptophan tRNA ligase
6.1.1.20 phenylalanine tRNA ligase
6.1.1.4 Leucine tRNA ligase
6.1.1.5 isoleucine tRNA ligase
6.1.1.6 Lysine tRNA ligase
6.1.1.7 alanine tRNA ligase
6.1.1.9 valine tRNA ligase
6.2.1.26 O-Succinylbenzoate-CoA ligase
6.2.1.3 long chain fatty acid CoA ligase
6.2.1.4 succinate CoA ligase (GTP-forming)
6.2.1.5 succinate CoA ligase (ADP-forming)
6.3.1.2 Glutamate ammonia ligase
6.3.1.5 NAD + synthase
6.3.2.1 Pantoat-β-alanine ligase
6.3.2.12 Dihydrofolate synthase
6.3.2.13 UDP-N-Acetylmuramoylalnyl-D-glutamate-2,6diaminopimelate ligase
6.3.2.15 UDP-N-acetylmuramoylalanyl-D-glutamyl-2,6-
6.3.2.17 tetrahydrofolyl polyglutamate synthase
6.3.2.4 D-Alanine-D-Alanine Ligase
6.3.2.6
Phosphoribosylaminoimidazolsuccinocarboxyamidsynthase
6.3.2.8 UDP-N-acetylmuramate alanine ligase
6.3.2.9 UDP-N-acetylmuramoylalanine-D-glutamate ligase
6.3.3.1 Phosphoribosyl-Formylglycinamidinecycloligase
6.3.3.3 Dethiobiotin synthase
6.3.4.13 Phosphoribosylamine glycine ligase
6.3.4.15 biotin [acetyl-CoA carboxylase] ligase
6.3.4.2 CTP synthase
6.3.4.4 Adenylosuccinate Synthase
6.3.4.5 argininosuccinate synthase
6.3.4.6 urea carboxylase
6.3.5.2 GMP synthase (glutamine hydrolyzing)
6.3.5.3 Phosphoribosylformylglycinamidine synthase
6.3.5.5 Carbamoyl phosphate synthase (glutamine hydrolyzing)
6.4.1.1 Pyruvate carboxylase
6.4.1.2 Acetyl-CoA carboxylase
6.4.1.3 Propionyl-CoA carboxylase
6.5.1.2 DNA ligase (NAD +). Bacillus subtilis enzymes with EC number 1.1.1 Alcohol dehydrogenase
1.1.1.100 3-oxoacyl- [acyl carrier protein] reductase
1.1.1.103 L-threonine-3-dehydrogenase
1.1.1.125 2-deoxy-D-gluconate-3-dehydrogenase
1.1.1.133 dTDP-4-dehydrorhamnose reductase
1.1.1.14 L-Iditol-2-dehydrogenase
1.1.1.140 sorbitol-6-phosphate-2-dehydrogenase
1.1.1.153 Sepiapterin reductase
1.1.1.169 2-dehydropantoate-2-reductase
1.1.1.17 mannitol-1-phosphate-5-dehydrogenase
1.1.1.18 Myoinositol-2-dehydrogenase
1.1.1.195 Cinnamyl alcohol dehydrogenase
1.1.1.2 Alcohol Dehydrogenase (NADP +)
1.1.1.202 1,3-propanediol dehydrogenase
1.1.1.204 xanthine dehydrogenase
1.1.1.205 IMP dehydrogenase
1.1.1.23 histidine dehydrogenase
1.1.1.236 Tropinone reductase
1.1.1.244 methanol dehydrogenase
1.1.1.25 Shikimate 5-dehydrogenase
1.1.1.27 L-lactate dehydrogenase
1.1.1.3 Homoserine Dehydrogenase
1.1.1.31 3-Hydroxyisobutyrate dehydrogenase
1.1.1.35 3-Hydroxyacyl-CoA dehydrogenase
1.1.1.36 Acetoacetyl-CoA reductase
1.1.1.37 malate dehydrogenase
1.1.1.38 Malate dehydrogenase (oxaloacetate decarboxylating)
1.1.1.40 Malate dehydrogenase (oxaloacetate decarboxylating) (NADP +)
1.1.1.42 isocitrate dehydrogenase (NADP +)
1.1.1.44 phosphogluconate dehydrogenase (decarboxylating)
1.1.1.47 glucose-1-dehydrogenase
1.1.1.49 glucose-6-phosphate-1-dehydrogenase
1.1.1.57 Fructuronate reductase
1.1.1.58 Tagaturonate reductase
1.1.1.69 Gluconate 5-dehydrogenase
1.1.1.8 Glycerol-3-phosphate dehydrogenase (NAD +)
1.1.1.83 D-malate dehydrogenase (decarboxylating)
1.1.1.85 3-isopropylmalate dehydrogenase
1.1.1.86 keto acid reductoisomerase
1.1.1.93 Tartrate dehydrogenase
1.1.1.95 Phosphoglycerate dehydrogenase
1.1.3.15 (S) -2-hydroxy acid oxidase
1.1.3.22 xanthine oxidase
1.1.99.5 Glycerol-3-phosphate dehydrogenase
1.1.99.8 alcohol dehydrogenase (acceptor)
1.10.2.2 Ubiquinol cytochrome c reductase
1.10.99.1 plastoquinol plastocyanin reductase
1.11.1.10 Chloride peroxidase
1.11.1.12 Phospholipid hydroperoxide glutathione peroxidase
1.11.1.6 Catalase
1.11.1.9 Glutathione peroxidase
1.13.11.32 2-nitropropane dioxygenase
1.14.13.3 4-Hydroxyphenylacetate 3-monooxygenase
1.14.13.39 Nitric oxide synthase
1.14.14.1 unspecific monooxygenase
1.15.1.1 Superoxide dismutase
1.17.4.1 Ribonucleoside Diphosphate Reductase
1.2.1.11 Aspartate semialdehyde dehydrogenase
1.2.1.12 Glyceraldehyde-3-phosphate dehydrogenase (phosphorylating)
1.2.1.13 Glyceraldehyde-3-phosphate dehydrogenase (NADP +) (phosphorylating)
1.2.1.16 succinate semialdehyde dehydrogenase (NAD (P) +)
1.2.1.2 Format dehydrogenase
1.2.1.27 methylmalonate semialdehyde dehydrogenase (acylating)
1.2.1.3 Aldehyde Dehydrogenase (NAD +)
1.2.1.38 N-Acetyl-γ-glutamyl phosphate reductase
1.2.1.4 Aldehyde Dehydrogenase (NADP +)
1.2.1.41 Glutamate 5-semialdehyde dehydrogenase
1.2.1.46 formaldehyde dehydrogenase
1.2.1.5 aldehyde dehydrogenase (NAD (P) +)
1.2.1.8 Betaine aldehyde dehydrogenase
1.2.2.2 Pyruvate dehydrogenase (cytochrome)
1.2.3.1 Aldehydroxidase
1.2.3.3 Pyruvate Oxidase
1.2.4.1 pyruvate dehydrogenase (lipoamide)
1.2.4.2 Oxoglutarate dehydrogenase (lipoamide)
1.2.4.4 3-methyl-2-oxobutanoate dehydrogenase (lipoamide)
1.2.99.2 carbon monoxide dehydrogenase
1.2.99.4 formaldehyde dismutase
1.3.1.12 Prephenate dehydrogenase
1.3.1.24 Biliverdin reductase
1.3.1.26 Dihydrodipicolinate reductase
1.3.1.28 2,3-Dihydro-2,3-dihydroxybenzoate dehydrogenase
1.3.1.34 2,4-dienoyl-CoA reductase (NADPH)
1.3.1.35 Phosphatidylcholine desaturase
1.3.1.43 Cyclohexadienyldehydrogenase
1.3.1.9 Enoyl- [Acyl Carrier Protein] reductase (NADH)
1.3.3.1 Dihydroorotate oxidase
1.3.3.4 Protoporphyrinogen oxidase
1.3.3.5 bilirubin oxidase
1.3.99.1 succinate dehydrogenase
1.3.99.3 Acyl-CoA dehydrogenase
1.4.1.1 alanine dehydrogenase
1.4.1.13 Glutamate Synthase (NADPH)
1.4.1.14 Glutamate Synthase (NADH)
1.4.1.2 Glutamate dehydrogenase
1.4.1.20 Phenylalanine dehydrogenase
1.4.1.3 Glutamate Dehydrogenase (NAD (P) +)
1.4.1.8 valine dehydrogenase (NADP +)
1.4.1.9 Leucine dehydrogenase
1.4.3.16 L-aspartate oxidase
1.4.3.2 L-amino acid oxidase
1.4.4.2 Glycine dehydrogenase (decarboxylating)
1.4.7.1 Glutamate Synthase (Ferredoxin)
1.5.1.12 1-pyrroline-5-carboxylate dehydrogenase
1.5.1.2 Pyrroline-5-carboxylate reductase
1.5.1.3 Dihydrofolate reductase
1.5.1.5 methylene tetrahydrofolate dehydrogenase (NADP +)
1.5.99.4 Nicotine dehydrogenase
1.5.99.8 Proline dehydrogenase
1.6.2.4 NADPH ferrihemoprotein reductase
1.6.4.5 Thioredoxin Reductase (NADPH)
1.6.5.3 NADH dehydrogenase (ubiquinone)
1.6.5.5 NADPH: quinone reductase
1.6.6.4 Nitrite reductase (NAD (P) H)
1.6.6.8 GMP reductase
1.6.8.2 NADPH dehydrogenase (flavin)
1.6.99.2 NAD (P) H-dehydrogenase (quinone)
1.6.99.3 NADH dehydrogenase
1.7.3.3 Urate Oxidase
1.7.99.4 nitrate reductase
1.7.99.7 Nitric oxide reductase
1.8.1.2 Sulfite reductase (NADPH)
1.8.1.4 Dihydrolipoamide dehydrogenase
1.8.4.6 Protein methionine S-oxide reductase
1.8.7.1 sulfite reductase (ferredoxin)
1.8.99.4
1.9.3.1 Cytochrome c oxidase
2.1.1.104 Caffeoyl-CoA O-methyltransferase
2.1.1.107 Uroporphyrin-III c-methyltransferase
2.1.1.14 5-methyltetrahydropteroyl triglutamate homocysteine
2.1.1.31 tRNA (guanine-N1 -) methyltransferase
2.1.1.37 DNA (cytosine 5 -) methyl transferase
2.1.1.45 thymidylate synthase
2.1.1.63 methylated-DNA-protein-cysteine-S-methyltransferase
2.1.1.73 sequence-specific DNA methyltransferase (cytosine-specific)
2.1.2.1 Glycine hydroxymethyl transferase
2.1.2.10 aminomethyl transferase
2.1.2.11 3-methyl-2-oxobutanoate hydroxymethyl transferase
2.1.2.2 Phosphoribosylglycinamide formyl transferase
2.1.2.3 Phosphoribosylaminoimidazole carboxamide formyl transferase
2.1.2.9 Methionyl tRNA formyl transferase
2.1.3.2 Aspartate carbamoyl transferase
2.1.3.3 ornithine carbamoyl transferase
2.2.1.1 Transketolase
2.2.1.2 Transaldolase
2.3.1.117 2,3,4,5-tetrahydropyridine-2-carboxylate N-succinyl transferase
2.3.1.118 N-hydroxyarylamine-O-acetyltransferase
2.3.1.12 Dihydrolipoamide S acetyltransferase
2.3.1.128 Ribosomal protein alanine N-acetyltransferase
2.3.1.16 Acetyl-CoA-C acyltransferase
2.3.1.18 Galactoside-O-acetyltransferase
2.3.1.19 Phosphate butyryl transferase
2.3.1.29 Glycine-C-acetyltransferase
2.3.1.30 Serine O-acetyltransferase
2.3.1.35 Glutamate-N-acetyltransferase
2.3.1.39 [Acyl Carrier Protein] -S-malonyltransferase
2.3.1.41 3-oxoacyl- [acyl carrier protein] synthase
2.3.1.46 Homoserine O-succinyl transferase
2.3.1.47 8-Amino-7-oxononanoate synthase
2.3.1.5 Arylamine-N-acetyltransferase
2.3.1.60 Gentamicin-3'-N-acetyltransferase
2.3.1.61 Dihydrolipoamide-S-succinyl transferase
2.3.1.8 Phosphate acetyl transferase
2.3.1.81 Aminoglycoside N3'-acetyltransferase
2.3.1.9 Acetyl-CoA-C acetyltransferase
2.3.2.13 Protein-glutamine-γ-glutamyltransferase
2.3.2.2 γ-glutamyl transferase
2.4.1.1 Phosphorylase
2.4.1.10 Levansucrase
2.4.1.17 Glucuronosyltransferase
2.4.1.18 1,4-α-glucan branching enzyme
2.4.1.21 Starch synthase
2.4.1.44 Lipopolysaccharide galactosyltransferase
2.4.1.46 1,2-Diacylglycerol-3-β-galactosyltransferase
2.4.1.52 Poly (glycerol phosphate) -α-glucosyltransferase
2.4.1.56 Lipopolysaccharide-N-acetylglucosaminyltransferase
2.4.2.1 Purine Nucleoside Phosphorylase
2.4.2.10 Orotate phosphoribosyl transferase
2.4.2.14 Amidophosphoribosyltransferase
2.4.2.17 ATP phosphoribosyl transferase
2.4.2.18 Anthranilate phosphoribosyl transferase
2.4.2.2 Pyrimidine nucleoside phosphorylase
2.4.2.29 Queuin tRNA ribosyltransferase
2.4.2.4 Thymidine phosphorylase
2.4.2.7 Adenine phosphoribosyl transferase
2.4.2.8 Hypoxanthine phosphoribosyl transferase
2.4.2.9 uracil phosphoribosyl transferase
2.5.1.10 Geranyltran transferase
2.5.1.15 Dihydropteroate synthase
2.5.1.16 Spermidine synthase
2.5.1.18 Glutathione transferase
2.5.1.19 3-Phosphoshikimate-1-carboxyvinyl transferase
5 2.5.1.29 Farnesyltransferase
2.5.1.3 Thiamine phosphate pyrophosphorylase
2.5.1.30 trans-hexaprenyltransferase
2.5.1.31 Di-trans, poly-cis-decaprenylcistransferase
2.5.1.6 Methionine adenosyltransferase
2.5.1.7 UDP-N-Acetylglucosamine-1-carboxyvinyltransferase
2.5.1.8 tRNA isopentenyl transferase
2.5.1.9 Riboflavin synthase
2.6.1.1 Aspartate Transaminase
2.6.1.11 Acetylornithine transaminase
2.6.1.13 Ornithine Oxo Acid Transaminase
2.6.1.16 glutamine-fructose-6-phosphate transaminase (isomerizing)
2.6.1.19 4-aminobutyrate transaminase 2.6.1.21 D-alanine transaminase
2.6.1.44 Alanine Glyoxylate Transaminase
2.6.1.51 Serine pyruvate transaminase
2.6.1.52 phosphoserine transaminase
2.6.1.62 Adenosylmethionine-8-amino-7-oxononanoate transaminase
2.6.1.9 Histidinol phosphate transaminase
2.7.1.11 6-phosphofructokinase
2.7.1.113 deoxyguanosine kinase
2.7.1.15 Ribokinase
2.7.1.16 Ribulokinase
2.7.1.17 xylulokinase
2.7.1.21 Thymidine kinase
2.7.1.25 Adenylyl sulfate kinase
2.7.1.30 glycerol kinase
2.7.1.33 Pantothenate kinase
2.7.1.37 protein kinase
2.7.1.39 Homoserine Kinase
2.7.1.4 Fructokinase
2.7.1.40 pyruvate kinase
2.7.1.47 D-Ribulokinase
2.7.1.48 uridine kinase
2.7.1.49 hydroxymethylpyrimidine kinase
2.7.1.5 rhamnulokinase
2.7.1.50 Hydroxyethylthiazole kinase
2.7.1.52 fucokinase
2.7.1.56 1-phosphofructokinase
2.7.1.6 Galactokinase
2.7.1.69 Protein-N (pai) -phosphohistidine-sugar-phosphotransferase
2.7.1.71 Shikimate kinase
2.7.1.76 deoxyadenosine kinase
2.7.2.1 acetate kinase
2.7.2.11 Glutamate 5-kinase
2.7.2.3 Phosphoglycerate kinase
2.7.2.4 Aspartate Kinase
2.7.2.7 Butyrate kinase
2.7.2.8 acetylglutamate kinase
2.7.3.3 Arginine Kinase
2.7.3.9 Phosphoenolpyruvate protein phosphotransferase
2.7.4.14 Cytidylate kinase
2.7.4.16 Thiamine phosphate kinase
2.7.4.3 Adenylate kinase
2.7.4.6 Nucleoside diphosphate kinase
2.7.4.8 guanylate kinase
2.7.6.1 Ribosephosphate Pyrophosphokinase
2.7.6.3 2-Amino-4-hydroxy-6-hydroxymethyldihydropteridinepyro-
2.7.6.5 GTP pyrophosphokinase
2.7.7.10 UTP hexose-1-phosphate uridylyl transferase
2.7.7.19 Polynucleotide adenylyl transferase
2.7.7.24 Glucose-1-phosphate-thymidylyl transferase
2.7.7.27 Glucose-1-phosphate adenylyl transferase
2.7.7.33 Glucose-1-phosphate cytidylyl transferase
2.7.7.39 Glycerol-3-phosphate cytidylyl transferase
2.7.7.4 Sulfate adenylyl transferase
2.7.7.41 Phosphatidate cytidylyl transferase
2.7.7.56 tRNA nucleotidyl transferase
2.7.7.6 DNA-related RNA polymerase
2.7.7.7 DNA-related DNA polymerase
2.7.7.8 Polyribonucleotide Nucleotidyl Transferase
2.7.7.9 UTP-glucose-1-phosphate uridylyl transferase
2.7.8.12 CDP-Glycerol-Glycerophosphotransferase
2.7.8.13 Phospho-N-acetylmuramoyl pentapeptide transferase
2.7.8.5 CDP-diacylglycerol-glycerol-3-phosphate-3-phosphatidyltransferase
2,7.8.7 holo [acyl carrier protein] synthase
2.7.8.8 CDP-diacylglycerol-serine-O-phosphatidyltransferase
2.7.9.2 Pyruvate water dikinase
2.8.3.5 3-Oxoacid-CoA transferase
2.8.3.9 Butyrate acetoacetate CoA transferase
3.1.1.1 Carboxylesterase
3.1.1.29 aminoacyl tRNA hydrolase
3.1.1.41 cephalosporin C deacetylase
3.1.1.61 protein glutamate methyl esterase
3.1.11.5 Exodesoxyribonuclease V
3.1.11.6 Exodesoxyribonuclease VII
3.1.2.2 Palmitoyl-CoA hydrolase
3.1.2.6 Hydroxyacylglutathione hydrolase
3.1.21.2 Deoxyribonuclease IV (phage T4-induced)
3.1.26.3 Ribonuclease III
3.1.26.4 calf thymus ribonuclease H
3.1.26.5 Ribonuclease P
3.1.3.1 alkaline phosphatase
3.1.3.11 Fructose bisphosphatase
3.1.3.16 Phosphoprotein phosphatase
3.1.3.18 phosphoglycolate phosphatase
3.1.3.25 Myoinositol-1 (or 4) monophosphatase
3.1.3.48 protein tyrosine phosphatase
3.1.3.5 5'-nucleotide
3.1.31.1 micrococcal nuclease
3.1.4.14 [Acyl Carrier Protein] phosphodiesterase
3.1.4.16 2 ', 3'-cyclic nucleotide 2'-phosphodiesterase
3.1.4.46 glycerophosphodiester phosphodiesterase
3.1.6.1 aryl sulfatase
3.2.1.1 α-amylase
3.2.1.10 Oligo-1,6-glucosidase
3.2.1.135 Neopullulanase
3.2.1.14 chitinase
3.2.1.20 α-glucosidase
3.2.1.21 β-glucosidase
3.2.1.22 α-galactosidase
3.2.1.23 β-galactosidase
3.2.1.25 β-mannosidase
3.2.1.26 β-fructofuranosidase
3.2.1.37 Xylan 1,4-β-xylosidase
3.2.1.4 Cellulase
3.2.1.41 α-dextrin-endo-1,6-α-glucosidase
3.2.1.48 Sucrose-α-glucosidase
3.2.1.52 β-N-acetylhexosaminidase
3.2.1.54 Cyclomaltodextrinase
3.2.1.55 α-N-arabinofuranosidase
3.2.1.65 Levanase
3.2.1.7 inulinase
3.2.1.73 Licheninase
3.2.1.74 Glucan-1,4-β-glucosidase
3.2.1.78 Mannan-endo-1,4-β-mannosidase
3.2.1.8 Endo-1,4-β-xylanase
3.2.1.86 6-phospho-β-glucosidase
3.2.1.93 α, α-phosphotrehalase
3.2.2.17 Deoxyribodipyrimidine donucleosidase
3.2.2.20 DNA-3-methyladenine glycosidase I
3.2.2.21 DNA-3-methyladenine glycosidase II
3.2.2.23 Formamidopyrimidine DNA glycosidase
3.3.2.1 Isochorismatase
3.3.2.3 Epoxy hydrolase
3.4.11.1 Leucyl aminopeptidase
3.4.11.18 Methionyl aminopeptidase
3.4.11.19 D-stereospecific aminopeptidase
3.4.11.5 Prolyl aminopeptidase
3.4.13.9 X-Pro dipeptidase
3.4.16.4 Serine type D-Ala, D-Ala carboxypeptidase
3.4.17.8 Muramoyl pentapeptide carboxypeptidase
3.4.19.1 Acylaminoacyl peptidase
3.4.19.3 Pyroglutamylpeptidase I
3.4.21.53 Endopeptidase Ia
3.4.21.62 Subtilisin
3.4.21.66 Thermitase
3.4.23.36 Signal peptidase II
3.4.24.27 Thermolysin
3.4.24.28 Bacillolysin
3.4.24.57 O-Syaloglycoprotein endopeptidase
3.4.99.36 lead peptidase
3.4.99.41 mitochondrial processing peptidase
3.5.1.1 Asparaginase
3.5.1.10 Formyl tetrahydrofolate deformylase
3.5.1.11 Penicillin amidase
3.5.1.16 acetylornithine deacetylase
3.5.1.2 Glutaminase
3.5.1.24 Choloylglycine hydrolase
3.5.1.25 N-acetylglucosamine-6-phosphate deacetylase
3.5.1.27 N-Formylmethionylaminoacyl-tRNA deformylase
3.5.1.28 N-acetylmuramoyl-L-alanine amidase
3.5.1.38 glutamine (asparagine) ase
3.5.1.5 urease
3.5.2.3 Dihydroorotase
3.5.2.5 Allantoinase
3.5.2.6 β-lactamase
3.5.3.1 Arginase
3.5.3.11 Agmatinase
3.5.4.10 IMP cyclohydrolase
3.5.4.12 dCMP deaminase
3.5.4.16 GTP cyclohydrolase I
3.5.4.19 Phosphoribosyl AMP cyclohydrolase
3.5.4.2 Adenine Deaminase
3.5.4.25 GTP cyclohydrolase II
3.5.4.5 cytidine deaminase
3.5.4.9 Methenyl tetrahydrofolate cyclohydrolase
3.6.1.23 dUTP pyrophosphatase
3.6.1.3 Adenosine triphosphatase
3.6.1.31 Phosphoribosyl-ATP pyrophosphatase
3.6.1.34 H + -transporting ATP synthase
3.6.1.35 H + transporting ATPase
3.6.1.38 Ca2 + transporting ATPase
3.6.1.41 bis (5'-nucleosyl) tetraphosphatase (symmetrical)
3.6.1.45 UDP sugar diphosphatase
3.6.1.7 Acylphosphatase
3.7.1.9 2-hydroxymuconate semialdehyde hydrolase
4.1.1.11 Aspartate 1 decarboxylase
4.1.1.17 ornithine decarboxylase
4.1.1.18 Lysine decarboxylase
4.1.1.19 arginine decarboxylase
4.1.1.20 diaminopimelate decarboxylase
4.1.1.21 phosphoribosylaminoimidazole carboxylase
4.1.1.23 Orotidine 5'-phosphate decarboxylase
4.1.1.37 uroporphyrinogen decarboxylase
4.1.1.39 Ribulose bisphosphate carboxylase
4.1.1.41 methylmalonyl-CoA decarboxylase
4.1.1.48 Indole-3-glycerol phosphate synthase
4.1.1.49 Phosphoenolpyruvate Carboxykinase (ATP)
4.1.1.5 Acetolactate decarboxylase
4.1.1.65 Phosphatidylserine decarboxylase
4.1.1.71 2-oxoglutarate decarboxylase
4.1.1.73 Tartrate decarboxylase
4.1.2.13 Fructose bisphosphate aldolase
4.1.2.14 2-Dehydro-3-deoxyphosphogluconate aldolase
4.1.2.15 2-Dehydro-3-deoxyphosphoheptonate aldolase
4.1.2.25 Dihydroneopterinaldolase
4.1.2.4 Deoxyribosephosphataldolase
4.1.3.12 2-isopropylmalate synthase
4.1.3.16 4-Hydroxy-2-oxoglutarataldolase
4.1.3.18 Acetolactate synthase
4.1.3.27 Anthranilate synthase
4.1.3.36 Naphthoate synthase
4.1.3.4 Hydroxymethylglutaryl-CoA lyase
4.1.3.7 Citrate (SI) synthase
4.2.1.10 3-dehydroquinate dehydratase
4.2.1.11 Phosphopyruvate hydratase
4.2.1.13 L-serine dehydratase
4.2.1.14 D-serine dehydratase
4.2.1.16 threonine dehydratase
4.2.1.17 Enoyl-CoA hydratase
4.2.1.19 Imidazole glycerol phosphate dehydratase
4.2.1.2 Fumarate hydratase
4.2.1.20 tryptophan synthase
4.2.1.24 Porphobilinogen synthase
4.2.1.3 Aconite hydratase
4.2.1.33 3-isopropylmalate dehydratase
4.2.1.40 glucarate dehydratase
4.2.1.41 5-Dehydro-4-deoxyglucarate dehydratase
4.2.1.46 dTDP-glucose-4,6-dehydratase
4.2.1.49 urocanate hydratase
4.2.1.51 Prephenate dehydratase
4.2.1.52 Dihydrodipicolinate synthase
4.2.1.7 Altronate dehydratase
4.2.1.75 Uroporphyrinogen III synthase
4.2.1.8 Mannonate dehydratase
4.2.1.9 Dihydroxy acid dehydratase
4.2.2.10 Pectin lyase
4.2.2.2 Pectate lyase
4.2.99.11 methylglyoxal synthase
4.2.99.18 DNA (apurinic or apyrimidine sequence) lyase
4.2.99.2 threonine synthase
4.2.99.8 cysteine synthase
4.3.1.1 Aspartate Ammonia Lyase
4.3.1.3 Histidine ammonia lyase
4.3.1.8 Hydroxymethylbilane Synthase
4.3.2.1 Argininosuccinate Lyase
4.3.2.2 Adenyl succinate lyase
4.4.1.17 Holocytochrome c synthase
4.4.1.5 Lactoylglutathione lyase
4.4.1.8 Cystathionin-β-lyase
4.6.1.3 3-dehydroquinate synthase
4.6.1.4 Chorismate synthase
4.99.1.1 Ferrochelatase
5.1.1.1 Alanine racemase
5.1.1.13 Aspartate racemase
5.1.1.3 Glutamate racemase
5.1.1.7 Diaminopimarate epimerase
5.1.2.2 Almond Racemase
5.1.3.1 Ribulosephosphate-3-epimerase
5.1.3.2 UDP-Glucose-4-epimerase
5.1.3.4 L-Ribulosephosphate-4-epimerase
5.2.1.8 Peptidyl prolyl isomerase
5.3.1.1 Triose phosphate isomerase
5.3.1.10 Glucosamine-6-phosphate isomerase
5.3.1.12 Glucuronate isomerase
5.3.1.14 L-rhamnose isomerase
5.3.1.16 N- (5'-Phospho-D-ribosylformimino) -5-amino-1-
5.3.1.17 4-deoxy-L-threo-5-hexosulose uronate ketoisomerase
5.3.1.24 Phosphoribosylanthranilate isomerase
5.3.1.4 L-arabinose isomerase
5.3.1.5 xylose isomerase
5.3.1.6 Ribose-5-phosphate epimerase
5.3.1.8 Mannose-6-phosphate isomerase
5.3.1.9 Glucose-6-phosphate isomerase
5.4.2.1 Phosphoglycerate mutase
5.4.2.6 β-phosphoglucomutase
5.4.2.7 Phosphopentomutase
5.4.3.8 Glutamate-1-semialdehyde-2,1-aminomutase
5.4.99.12 tRNA pseudouridine synthase I
5.4.99.5 Chorismate mutase
5.4.99.6 isochorismate synthase
5.4.99.7 Lanosterol synthase
5.5.1.1 Muconate cycloisomerase
5.5.1.7 Chloromuconate cycloisomerase
5.99.1.2 DNA topoisomerase
5.99.1.3 DNA topoisomerase (ATP hydrolyzing)
6.1.1.1 Tyrosine tRNA ligase
6.1.1.10 methionine tRNA ligase
6.1.1.11 Serine tRNA ligase
6.1.1.12 Aspartate tRNA ligase
6.1.1.14 glycine tRNA ligase
6.1.1.15 Proline tRNA ligase
6.1.1.16 cysteine tRNA ligase
6.1.1.17 glutamate tRNA ligase
6.1.1.19 arginine tRNA ligase
6.1.1.2 Tryptophan tRNA ligase
6.1.1.20 phenylalanine tRNA ligase
6.1.1.21 histidine tRNA ligase
6.1.1.22 Asparagine tRNA ligase
6.1.1.3 Threonine tRNA ligase
6.1.1.4 Leucine tRNA ligase
6.1.1.5 isoleucine tRNA ligase
6.1.1.6 Lysine tRNA ligase
6.1.1.7 alanine tRNA ligase
6.1.1.9 valine tRNA ligase
6.2.1.1 Acetate-CoA ligase
6.2.1.14 6-carboxyhexanoate CoA ligase
6.2.1.26 O-Succinylbenzoate-CoA ligase
6.2.1.3 long chain fatty acid CoA ligase
6.2.1.4 succinate CoA ligase (GTP-forming)
6.2.1.5 succinate CoA ligase (ADP-forming)
6.3.1.2 Glutamate ammonia ligase
6.3.1.5 NAD + synthase
6.3.2.1 Pantoat-β-alanine ligase
6.3.2.13 UDP-N-Acetylmuramoylalny1-D-Glutamate-2,6-diaminopimelate ligase
6.3.2.15 UDP-N-acetylmuramoylalanyl-D-glutamyl-2,6-
6.3.2.17 tetrahydrofolyl polyglutamate synthase
6.3.2.4 D-Alanine-D-Alanine Ligase
6.3.2.6 Phosphoribosylaminoimidazole succinocarboxyamide synthase
6.3.2.8 UDP-N-acetylmuramate alanine ligase
6.3.2.9 UDP-N-acetylmuramoylalanine-D-glutamate ligase
6.3.3.1 Phosphoribosyl-Formylglycinamidinecycloligase
6.3.3.2 5-Formyltetrahydrofolatecycloligase
6.3.3.3 Dethiobiotin synthase
6.3.4.13 Phosphoribosylamine glycine ligase
6.3.4.14 Biotin carboxylase
6.3.4.2 CTP synthase
6.3.4.4 Adenylosuccinate Synthase
6.3.4.5 argininosuccinate synthase
6.3.5.1 NAD + synthase (glutamine hydrolyzing)
6.3.5.2 GMP synthase (glutamine hydrolyzing)
6.3.5.3 Phosphoribosylformylglycinamidine synthase
6.3.5.4 asparagine synthase (glutamine hydrolyzing)
6.3.5.5 Carbamoyl phosphate synthase (glutamine hydrolyzing)
6.4.1.1 Pyruvate carboxylase
6.4.1.2 Acetyl-CoA carboxylase
6.5.1.2 DNA ligase (NAD +). Lactococcus lactis enzymes with EC number 1.1.1.1 Alcohol dehydrogenase
1.1.1.100 3-oxoacyl- [acyl carrier protein] reductase
1.1.1.14 L-Iditol-2-dehydrogenase
1.1.1.169 2-dehydropantoate-2-reductase
1.1.1.17 mannitol-1-phosphate-5-dehydrogenase
1.1.1.184 carbonyl reductase (NADPH)
1.1.1.195 Cinnamyl alcohol dehydrogenase
1.1.1.205 IMP dehydrogenase
1.1.1.218 morphine-6-dehydrogenase
1.1.1.219 Dihydrokaempferol-4-reductase
1.1.1.23 histidine dehydrogenase
1.1.1.25 Shikimate 5-dehydrogenase
1.1.1.27 L-lactate dehydrogenase
1.1.1.3 Homoserine Dehydrogenase
1.1.1.31 3-Hydroxyisobutyrate dehydrogenase
1.1.1.38 Malate dehydrogenase (oxaloacetate decarboxylating)
1.1.1.4 (R, R) -butanediol dehydrogenase
1.1.1.40 Malate dehydrogenase (oxaloacetate decarboxylating) (NADP +)
1.1.1.42 isocitrate dehydrogenase (NADP +)
1.1.1.44 phosphogluconate dehydrogenase (decarboxylating)
1.1.1.49 glucose-6-phosphate-1-dehydrogenase
1.1.1.53 3-α (or 20-β-hydroxysteroid dehydrogenase
1.1.1.57 Fructuronate reductase
1.1.1.6 Glycerol dehydrogenase
1.1.1.8 Glycerol-3-phosphate dehydrogenase (NAD +)
1.1.1.85 3-isopropylmalate dehydrogenase
1.1.1.86 keto acid reductoisomerase
1.1.1.88 hydroxymethylglutaryl-CoA reductase
1.1.1.95 Phosphoglycerate dehydrogenase
1.1.3.15 (S) -2-hydroxy acid oxidase
1.1.99.5 Glycerol-3-phosphate dehydrogenase
1.11.1.1 NADH peroxidase
1.11.1.9 Glutathione peroxidase
1.12.2.1 Cytochrome c3 hydrogenase
1.15.1.1 Superoxide dismutase
1.16.1.1 Mercury (II) reductase
1.17.4.1 Ribonucleoside Diphosphate Reductase
1.17.4.2 Ribonucleoside triphosphate reductase
1.2.1.10 acetaldehyde dehydrogenase (acetylating)
1.2.1.11 Aspartate semialdehyde dehydrogenase
1.2.1.12 Glyceraldehyde-3-phosphate dehydrogenase (phosphorylating)
1.2.1.2 Format dehydrogenase
1.2.1.3 Aldehyde Dehydrogenase (NAD +)
1.2.1.38 N-Acetyl-γ-glutamyl phosphate reductase
1.2.1.41 Glutamate 5-semialdehyde dehydrogenase
1.2.2.2 Pyruvate dehydrogenase (cytochrome)
1.2.3.3 Pyruvate Oxidase
1.2.4.1 pyruvate dehydrogenase (lipoamide)
1.2.4.4 3-methyl-2-oxobutanoate dehydrogenase (lipoamide)
1.3.1.12 Prephenate dehydrogenase
1.3.1.26 Dihydrodipicolinate reductase
1.3.1.9 Enoyl- [Acyl Carrier Protein] reductase (NADH)
1.3.3.1 Dihydroorotate oxidase
1.3.99.1 succinate dehydrogenase
1.4.1.1 alanine dehydrogenase
1.4.1.13 Glutamate Synthase (NADPH)
1.4.1.14 Glutamate Synthase (NADH)
1.4.7.1 Glutamate Synthase (Ferredoxin)
1.5.1.2 Pyrroline-5-carboxylate reductase
1.5.1.24 N5- (carboxyethyl) ornithine synthase
1.5.1.3 Dihydrofolate reductase
1.5.1.5 methylene tetrahydrofolate dehydrogenase (NADP +)
1.6.4.2 Glutathione reductase (NADPH)
1.6.4.5 Thioredoxin Reductase (NADPH)
1.6.5.3 NADH dehydrogenase (ubiquinone)
1.6.6.8 GMP reductase
1.7.99.5 5,10-methylene tetrahydrofolate reductase (FADH2)
1.8.1.4 Dihydrolipoamide dehydrogenase
1.97.1.4 [Pyruvate format lyase] activating enzyme
2.1.1.104 Caffeoyl-CoA-O-methyltransferase
2.1.1.14 5-methyltetrahydropteroyl triglutamate homocysteine
2.1.1.31 tRNA- (guanine-N1-) methyltransferase
2.1.1.45 thymidylate synthase
2.1.1.63 methylated-DNA-protein-cysteine-S-methyltransferase
2.1.2.1 Glycine hydroxymethyl transferase
2.1.2.2 Phosphoribosylglycinamide formyl transferase
2.1.2.3 Phosphoribosylaminoimidazole carboxamide formyl transferase
2.1.2.9 Methionyl tRNA formyl transferase
2.1.3.2 Aspartate carbamoyl transferase
2.1.3.3 ornithine carbamoyl transferase
2.2.1.1 Transketolase
2.3.1.12 Dihydrolipoamide S-acetyltransferase
2.3.1.128 Ribosomal protein alanine N-acetyltransferase
2.3.1.18 Galactoside-O-acetyltransferase
2.3.1.30 Serine O-acetyltransferase
2.3.1.35 Glutamate-N-acetyltransferase
2.3.1.39 [Acyl Carrier Protein] -S-malonyltransferase
2.3.1.41 3-Oxoacyl [acyl carrier protein] synthase
2.3.1.46 Homoserine-O-succinyl transferase
2.3.1.54 Format c-acetyltransferase
2.3.1.57 Diamine N-acetyltransferase
2.3.1.8 Phosphate acetyl transferase
2.3.1.9 Acetyl-CoA-C acetyltransferase
2.4.1.1 Phosphorylase
2.4.1.21 Starch synthase
2.4.1.24 1,4-α-glucan-6-α-glucosyltransferase
2.4.1.25 4-α-glucanotransferase
2.4.1.52 Poly (glycerol phosphate) -α-glucosyltransferase
2.4.2.1 Purine Nucleoside Phosphorylase
2.4.2.10 Orotate phosphoribosyl transferase
2.4.2.14 Amidophosphoribosyltransferase
2.4.2.17 ATP phosphoribosyl transferase
2.4.2.18 Anthranilate phosphoribosyl transferase
2.4.2.2 Pyrimidine nucleoside phosphorylase
2.4.2.29 Queuin tRNA ribosyltransferase
2.4.2.3 Uridine phosphorylase
2.4.2.4 Thymidine phosphorylase
2.4.2.7 Adenine phosphoribosyl transferase
2.4.2.8 Hypoxanthine phosphoribosyl transferase
2.4.2.9 uracil phosphoribosyl transferase
2.5.1.10 Geranyltran transferase
2.5.1.15 Dihydropteroate synthase
2.5.1.19 3-Phosphoshikimate-1-carboxyvinyl transferase
2.5.1.29 Farnesyltransferase
2.5.1.3 Thiamine phosphate pyrophosphorylase
2.5.1.31 Di-trans, poly-cis-decaprenylcistransferase
2.5.1.6 Methionine adenosyltransferase
2.5.1.7 UDP-N-Acetylglucosamine-1-carboxyvinyltransferase
2.5.1.8 tRNA isopentenyl transferase
2.5.1.9 Riboflavin synthase
2.6.1.1 Aspartate Transaminase
2.6.1.16 glutamine-fructose-6-phosphate transaminase (isomerizing)
2.6.1.45 Serine glyoxylate transaminase
2.6.1.52 phosphoserine transaminase
2.6.1.9 Histidinol phosphate transaminase
2.7.1.107 diacylglycerol kinase
2.7.1.11 6-phosphofructokinase
2.7.1.113 deoxyguanosine kinase
2.7.1.15 Ribokinase
2.7.1.17 xylulokinase
2.7.1.21 Thymidine kinase
2.7.1.30 glycerol kinase
2.7.1.33 Pantothenate kinase
2.7.1.36 Mevalonate kinase
2.7.1.39 Homoserine Kinase
2.7.1.4 Fructokinase
2.7.1.40 pyruvate kinase
2.7.1.48 uridine kinase
2.7.1.49 hydroxymethylpyrimidine kinase
2.7.1.50 Hydroxyethylthiazole kinase
2.7.1.56 1-phosphofructokinase
2.7.1.6 Galactokinase
2.7.1.69 Protein-N (pai) -phosphohistidine-sugar-phosphotransferase
2.7.1.71 Shikimate kinase
2.7.1.76 deoxyadenosine kinase
2.7.1.95 Kanamycin kinase
2.7.2.1 acetate kinase
2.7.2.11 Glutamate 5-kinase
2.7.2.2 carbamate kinase
2.7.2.3 Phosphoglycerate kinase
2.7.2.4 Aspartate Kinase
2.7.2.8 acetylglutamate kinase
2.7.3.9 Phosphoenolpyruvate protein phosphotransferase
2.7.4.14 Cytidylate kinase
2.7.4.3 Adenylate kinase
2.7.4.8 guanylate kinase
2.7.6.1 Ribosephosphate Pyrophosphokinase
2.7.6.5 GTP pyrophosphokinase
2.7.7.10 UTP hexose-1-phosphate uridylyl transferase
2.7.7.24 Glucose-1-phosphate-thymidylyl transferase
2.7.7.27 Glucose-1-phosphate adenylyl transferase
2.7.7.39 Glycerol-3-phosphate cytidylyl transferase
2.7.7.41 Phosphatidate cytidylyl transferase
2.7.7.6 DNA-related RNA polymerase
2.7.7.7 DNA-related DNA polymerase
2.7.7.8 Polyribonucleotide Nucleotidyl Transferase
2.7.7.9 UTP-glucose-1-phosphate uridylyl transferase
2.7.8.12 CDP-Glycerol-Glycerophosphotransferase
2.7.8.13 Phospho-N-acetylmuramoylpentapeptide transferase
2.7.8.5 CDP-diacylglycerol-glycerol-3-phosphate 3-phosphatidyl transferase
2.7.8.7 Holo [acyl carrier protein] synthase
3.1.1.29 aminoacyl tRNA hydrolase
3.1.11.5 Exodesoxyribonuclease V
3.1.11.6 Exodesoxyribonuclease VII
3.1.21.3 Type I sequence-specific deoxyribonuclease
3.1.26.3 Ribonuclease III
3.1.26.4 calf thymus ribonuclease H
3.1.26.5 Ribonuclease P
3.1.3.11 Fructose bisphosphatase
3.1.3.15 histidinol phosphatase
3.1.3.16 Phosphoprotein phosphatase
3.1.3.25 Myoinositol-1 (or 4) monophosphatase
3.1.3.3 Phosphoserine Phosphatase
3.1.3.48 protein tyrosine phosphatase
3.1.3.5 5'-nucleotide
3.1.4.14 [Acyl Carrier Protein] phosphodiesterase
3.1.4.16 2 ', 3'-cyclic nucleotide 2'-phosphodiesterase
3.1.4.46 glycerophosphodiester phosphodiesterase
3.1.6.1 aryl sulfatase
3.1.7.2 Guanosine-3 ', 5'-bis (diphosphate) -3'-pyrophosphatase
3.2.1.1 α-amylase
3.2.1.10 Oligo-1,6-glucosidase
3.2.1.135 Neopullulanase
3.2.1.14 chitinase
3.2.1.20 α-glucosidase
3.2.1.21 β-glucosidase
3.2.1.23 β-galactosidase
3.2.1.28 α, α-trehalase
3.2.1.37 xylan-1,4-β-xylosidase
3.2.1.41 α-dextrin-endo-1,6-α-glucosidase
3.2.1.52 β-N-acetylhexosaminidase
3.2.1.54 Cyclomaltodextrinase
3.2.1.70 glucan-1,6-α-glucosidase
3.2.1.8 Endo-1,4-β-xylanase
3.2.1.86 6-phospho-β-glucosidase
3.2.1.93 α, α-phosphotrehalase
3.2.1.96 Mannosylglycoprotein-endo-β-N-
acetylglucosaminidase
3.2.2.17 Deoxyribodipyrimidine donucleosidase
3.2.2.23 Formamidopyrimidine DNA glycosidase
3.4.11.15 Lysyl aminopeptidase
3.4.11.18 Methionyl aminopeptidase
3.4.11.2 Membrane alanyl aminopeptidase
3.4.11.5 Prolyl aminopeptidase
3.4.13.18 Cytosol non-specific dipeptidase
3.4.13.9 X-Pro dipeptidase
3.4.14.11 XAA-Pro-Dipeptidylpeptidase
3.4.16.4 Serine type D-Ala, D-Ala carboxypeptidase
3.4.23.36 Signal peptidase II
3.4.24.57 O-Syaloglycoprotein endopeptidase
3.4.99.36 lead peptidase
3.4.99.41 mitochondrial processing peptidase
3.5.1.1 Asparaginase
3.5.1.11 Penicillin amidase
3.5.1.16 acetylornithine deacetylase
3.5.1.18 succinyldiaminopimelate desuccinylase
3.5.1.24 Choloylglycine hydrolase
3.5.1.25 N-acetylglucosamine-6-phosphate deacetylase
3.5.2.12 6-aminohexanoate cyclic dimer hydrolase
3.5.2.3 Dihydroorotase
3.5.3.6 arginine deiminase
3.5.4.10 IMP cyclohydrolase
3.5.4.12 dCMP deaminase
3.5.4.16 GTP cyclohydrolase I
3.5.4.19 Phosphoribosyl AMP cyclohydrolase
3.5.4.25 GTP cyclohydrolase II
3.5.4.4 Adenosine Deaminase
3.5.4.5 cytidine deaminase
3.5.4.9 Methenyl tetrahydrofolate cyclohydrolase
3.6.1.17 bis (5'-nucleosyl) tetraphosphatase (asymmetric)
3.6.1.23 dUTP pyrophosphatase
3.6.1.3 Adenosine triphosphatase
3.6.1.31 Phosphoribosyl-ATP pyrophosphatase
3.6.1.34 H + -transporting ATP synthase
3.6.1.35 H + transporting ATPase
3.6.1.36 H + / K + exchanging ATPase
3.6.1.38 Ca2 + transporting ATPase
4.1.1.1 Pyruvate decarboxylase
4.1.1.15 glutamate decarboxylase
4.1.1.20 diaminopimelate decarboxylase
4.1.1.21 phosphoribosylaminoimidazole carboxylase
4.1.1.23 Orotidine 5'-phosphate decarboxylase
4.1.1.33 Diphosphomevalonate decarboxylase
4.1.1.44 4-Carboxymuconolactone decarboxylase
4.1.1.48 Indole-3-glycerol phosphate synthase
4.1.1.5 Acetolactate decarboxylase
4.1.1.71 2-oxoglutarate decarboxylase
4.1.2.13 Fructose bisphosphate aldolase
4.1.2.15 2-Dehydro-3-deoxyphosphoheptonate aldolase
4.1.2.25 Dihydroneopterinaldolase
4.1.2.4 Deoxyribosephosphataldolase
4.1.3.18 Acetolactate synthase
4.1.3.27 Anthranilate synthase
4.1.3.36 Naphthoate synthase
4.1.3.5 Hydroxymethylglutaryl-CoA synthase
4.1.3.6 Citrate lyase
4.1.3.7 Citrate (SI) synthase
4.2.1.10 3-dehydroquinate dehydratase
4.2.1.11 Phosphopyruvate hydratase
4.2.1.12 Phosphogluconate dehydratase
4.2.1.13 L-serine dehydratase
4.2.1.16 threonine dehydratase
4.2.1.19 Imidazole glycerol phosphate dehydratase
4.2.1.20 tryptophan synthase
4.2.1.3 Aconite hydratase
4.2.1.33 3-isopropylmalate dehydratase
4.2.1.51 Prephenate dehydratase
4.2.1.52 Dihydrodipicolinate synthase
4.2.1.8 Mannonate dehydratase
4.2.1.9 Dihydroxy acid dehydratase
4.2.99.18 DNA (apurinic or apyrimidine sequence) lyase
4.2.99.2 threonine synthase
4.2.99.8 cysteine synthase
4.2.99.9 O-succinyl homoserine (thiol) lyase
4.3.2.1 Argininosuccinate Lyase
4.3.2.2 Adenyl succinate lyase
4.6.1.3 3-dehydroquinate synthase
4.6.1.4 Chorismate synthase
4.99.1.1 Ferrochelatase
5.1.1.1 Alanine racemase
5.1.1.13 Aspartate racemase
5.1.1.3 Glutamate racemase
5.1.3.1 Ribulosephosphate-3-epimerase
5.1.3.2 UDP-Glucose-4-epimerase
5.1.3.3 Aldose-1-epimerase
5.2.1.8 Peptidyl prolyl isomerase
5.3.1.1 Triose phosphate isomerase
5.3.1.10 Glucosamine-6-phosphate isomerase
5.3.1.12 Glucuronate isomerase
5.3.1.16 N- (5'-Phospho-D-ribosylformimino) -5-amino-1-
5.3.1.24 Phosphoribosylanthranilate isomerase
5.3.1.4 L-arabinose isomerase
5.3.1.5 xylose isomerase
5.3.1.6 Ribose-5-phosphate epimerase
5.3.1.8 Mannose-6-phosphate isomerase
5.3.1.9 Glucose-6-phosphate isomerase
5.4.2.1 Phosphoglycerate mutase
5.4.2.6 β-phosphoglucomutase
5.4.2.7 Phosphopentomutase
5.4.2.8 Phosphomannomutase
5.4.99.12 tRNA pseudouridine synthase I
5.4.99.5 Chorismate mutase
5.4.99.6 isochorismate synthase
5.5.1.7 Chloromuconate cycloisomerase
5.99.1.3 DNA topoisomerase (ATP hydrolyzing)
6.1.1.1 Tyrosine tRNA ligase
6.1.1.10 methionine tRNA ligase
6.1.1.11 Serine tRNA ligase
6.1.1.12 Aspartate tRNA ligase
6.1.1.14 glycine tRNA ligase
6.1.1.15 Proline tRNA ligase
6.1.1.16 cysteine tRNA ligase
6.1.1.17 glutamate tRNA ligase
6.1.1.19 arginine tRNA ligase
6.1.1.2 Tryptophan tRNA ligase
6.1.1.20 phenylalanine tRNA ligase
6.1.1.21 histidine tRNA ligase
6.1.1.22 Asparagine tRNA ligase
6.1.1.3 Threonine tRNA ligase
6.1.1.4 Leucine tRNA ligase
6.1.1.5 isoleucine tPNA ligase
6.1.1.6 Lysine tRNA ligase
6.1.1.7 alanine tRKA ligase
6.1.1.9 valine tRNA ligase
6.2.1.1 Acetate-CoA ligase
6.2.1.22 Citrate (Pro-3S) lyase ligase
6.2.1.26 O-Succinylbenzoate-CoA ligase
6.2.1.3 long chain fatty acid CoA ligase
6.3.1.1 Aspartate ammonia ligase
6.3.1.2 Glutamate ammonia ligase
6.3.1.5 NAD + synthase
6.3.2.13 UDP-N-acetylmuramoylalnyl-D-glutamate-2,6-diaminopimelate ligase
6.3.2.15 UDP-N-acetylmuramoylalanyl-D-glutamyl-2,6-
6.3.2.17 tetrahydrofolyl polyglutamate synthase
6.3.2.4 D-Alanine-D-Alanine Ligase
6.3.2.6 Phosphoribosylaminoimidazole succinocarboxyamide synthase
6.3.2.8 UDP-N-acetylmuramate alanine ligase
6.3.2.9 UDP-N-acetylmuramoylalanine-D-glutamate ligase
6.3.3.1 Phosphoribosyl-Formylglycinamidinecycloligase
6.3.4.13 Phosphoribosylamine glycine ligase
6.3.4.14 Biotin carboxylase
6.3.4.15 biotin [acetyl-CoA carboxylase] ligase
6.3.4.2 CTP synthase
6.3.4.3 Format tetrahydrofolate ligase
6.3.4.4 Adenylosuccinate Synthase
6.3.4.5 argininosuccinate synthase
6.3.5.1 NAD + synthase (glutamine hydrolyzing)
6.3.5.2 GMP synthase (glutamine hydrolyzing)
6.3.5.3 Phosphoribosylformylglycinamidine synthase
6.3.5.4 asparagine synthase (glutamine hydrolyzing)
6.3.5.5 Carbamoyl phosphate synthase (glutamine hydrolyzing)
6.4.1.1 Pyruvate carboxylase
6.4.1.2 Acetyl-CoA carboxylase
6.5.1.2 DNA ligase (NAD +). Enzymes from Clostridium acetobutylicum with EC number 1.1.1.1 Alcohol dehydrogenase
1.1.1.100 3-oxoacyl- [acyl carrier protein] reductase
1.1.1.125 2-deoxy-D-gluconate-3-dehydrogenase
1.1.1.169 2-dehydropantoate-2-reductase
1.1.1.17 mannitol-1-phosphate-5-dehydrogenase
1.1.1.195 Cinnamyl alcohol dehydrogenase
1.1.1.202 1,3-propanediol dehydrogenase
1.1.1.205 IMP dehydrogenase
1.1.1.211 long chain hydroxyacyl-CoA dehydrogenase
1.1.1.219 Dihydrokaempferol-4-reductase
1.1.1.23 histidine dehydrogenase
1.1.1.25 Shikimate 5-dehydrogenase
1.1.1.27 L-lactate dehydrogenase
1.1.1.28 D-lactate dehydrogenase
1.1.1.29 Glycerate dehydrogenase
1.1.1.3 Homoserine Dehydrogenase
1.1.1.31 3-Hydroxyisobutyrate dehydrogenase
1.1.1.35 3-Hydroxyacyl-CoA dehydrogenase
1.1.1.37 malate dehydrogenase
1.1.1.38 Malate dehydrogenase (oxaloacetate decarboxylating)
1.1.1.40 Malate dehydrogenase (oxaloacetate decarboxylating) (NADP +)
1.1.1.41 isocitrate dehydrogenase (NAD +)
1.1.1.47 glucose-1-dehydrogenase
1.1.1.57 Fructuronate reductase
1.1.1.58 Tagaturonate reductase
1.1.1.69 Gluconate 5-dehydrogenase
1.1.1.8 Glycerol-3-phosphate dehydrogenase (NAD +)
1.1.1.85 3-isopropylmalate dehydrogenase
1.1.1.86 keto acid reductoisomerase
1.1.1.95 Phosphoglycerate dehydrogenase
1.1.99.6 D-2-hydroxy acid dehydrogenase
1.1.99.8 alcohol dehydrogenase (acceptor)
1.10.2.2 Ubiquinol cytochrome c reductase
1.11.1.10 Chloride peroxidase
1.11.1.12 Phospholipid hydroperoxide glutathione peroxidase
1.11.1.9 Glutathione peroxidase
1.12.1.2 Hydrogen dehydrogenase
1.12.2.1 Cytochrome c3 hydrogenase
1.12.99.1 Coenzyme f420 hydrogenase
1.15.1.1 Superoxide dismutase
1.17.4.1 Ribonucleoside Diphosphate Reductase
1.17.4.2 Ribonucleoside triphosphate reductase
1.18.1.1 Rubredoxin NAD + reductase
1.18.6.1 nitrogenase
1.18.99.1 hydrogenase
1.2.1.10 acetaldehyde dehydrogenase (acetylating)
1.2.1.11 Aspartate semialdehyde dehydrogenase
1.2.1.12 Glyceraldehyde-3-phosphate dehydrogenase (phosphorylating)
1.2.1.2 Format dehydrogenase
1.2.1.38 N-Acetyl-γ-glutamyl phosphate reductase
1.2.1.41 Glutamate 5-semialdehyde dehydrogenase
1.2.1.9 Glyceraldehyde-3-phosphate dehydrogenase (NADP +)
1.2.7.1 Pyruvate Synthase
1.2.99.2 carbon monoxide dehydrogenase
1.3.1.12 Prephenate dehydrogenase
1.3.1.26 Dihydrodipicolinate reductase
1.3.1.43 Cyclohexadienyldehydrogenase
1.3.3.1 Dihydroorotate oxidase
1.3.99.1 succinate dehydrogenase
1.3.99.3 Acyl-CoA dehydrogenase
1.4.1.13 Glutamate Synthase (NADPH)
1.4.1.14 Glutamate Synthase (NADH)
1.4.1.4 Glutamate Dehydrogenase (NADP +)
1.4.3.16 L-aspartate oxidase
1.4.7.1 glutamate synthase (ferredoxin)
1.5.1.2 Pyrroline-5-carboxylate reductase
1.5.1.3 Dihydrofolate reductase
1.5.1.5 methylenetetrahydrofolate dehydrogenase (NADP +)
1.6.4.5 Thioredoxin Reductase (NADPH)
1.6.6.4 Nitrite reductase (NAD (P) H)
1.6.6.8 GMP reductase
1.6.99.2 NAD (P) H-dehydrogenase (quinone)
1.7.7.1 Ferredoxin nitrite reductase
1.7.7.2 Ferredoxin nitrate reductase
1.8.4.6 Protein methianin S-oxide reductase
1.8.99.2 adenylyl sulfate reductase
1.97.1.4 [Pyruvate format lyase] activating enzyme
1.97.4.1
2.1.1.104 Caffeoyl-CoA-O-methyltransferase
2.1.1.107 Uroporphyrin-III c-methyltransferase
2.1.1.13 5-methyltetrahydrofolate homocysteine S-methyltransferase
2.1.1.31 tRNA- (guanine-N1-) methyltransferase
2.1.1.37 THEN- (cytosine-5 -) methyltransferase
2.1.1.45 thymidylate synthase
2.1.1.63 methylated-DNA-protein-cysteine-S-methyltransferase
2.1.1.72 sequence-specific DANN-methyltransferase (adenine-specific)
2.1.1.73 sequence-specific DANN-methyltransferase (cytosine-specific)
2.1.1.79 Cyclopropane fatty acid acyl phospholipid synthase
2.1.1.80 protein glutamate-O-methyltransferase
2.1.2.1 Glycine hydroxymethyl transferase
2.1.2.11 3-methyl-2-oxobutanoate hydroxymethyl transferase
2.1.2.2 Phosphoribosylglycinamide formyl transferase
2.1.2.3 Phosphoribosylaminoimidazole carboxamide formyl transferase
2.1.2.5 Glutamate formiminotransferase
2.1.2.9 Methionyl tRNA formyl transferase
2.1.3.2 Aspartate carbamoyl transferase
2.1.3.3 ornithine carbamoyl transferase
2.2.1.1 Transketolase
2.2.1.2 Transaldolase
2.3.1.117 2,3,4,5-tetrahydropyridine-2-carboxylate-N-succinyl transferase
2.3.1.128 Ribosomal protein alanine N-acetyltransferase
2.3.1.18 Galactoside-O-acetyltransferase
2.3.1.19 Phosphate butyryl transferase
2.3.1.28 Chloramphenicol-O-acetyltransferase
2.3.1.30 Serine O-acetyltransferase
2.3.1.35 Glutamate-N-acetyltransferase
2.3.1.39 [Acyl Carrier Protein] -S-malonyltransferase
2.3.1.41 3-oxoacyl- [acyl carrier protein] synthase
2.3.1.46 Homoserine O-succinyl transferase
2.3.1.51 1-Acylglycerol-3-phosphate-O-acyltransferase
2.3.1.54 Format c-acetyltransferase
2.3.1.8 Phosphate acetyl transferase
2.3.1.85 fatty acid synthase
2.3.1.9 Acetyl-CoA-C acetyltransferase
2.4.1.1 Phosphorylase
2.4.1.10 Levansucrase
2.4.1.117 Dolichylphosphate-β-glucosyltransferase
2.4.1.12 Cellulose Synthase (Forming UDP)
2.4.1.17 Glucuronosyltransferase
2.4.1.19 Cyclomaltodextrin glucanotransferase
2.4.1.21 Starch synthase
2.4.1.46 1,2-Diacylglycerol-3-β-galactosyltransferase
2.4.1.83 Dolichylphosphate-β-D-mannosyltransferase
2.4.2.1 Purine Nucleoside Phosphorylase
2.4.2.10 Orotate phosphoribosyl transferase
2.4.2.14 Amidophosphoribosyltransferase
2.4.2.17 ATP phosphoribosyl transferase
2.4.2.18 Anthranilate phosphoribosyl transferase
2.4.2.2 Pyrimidine nucleoside phosphorylase
2.4.2.21 Nicotinate Nucleotide Dimethylbenzimidazole Phosphoribosyl
2.4.2.29 Queuin tRNA ribosyltransferase
2.4.2.4 Thymidine phosphorylase
2.4.2.7 Adenine phosphoribosyl transferase
2.4.2.8 Hypoxanthine phosphoribosyl transferase
2.4: 2.9 uracil phosphoribosyl transferase
2.4.99.1 β-galactoside-α-2,6-sialyltransferase
2.5.1.10 Geranyltran transferase
2.5.1.15 Dihydropteroate synthase
2.5.1.16 Spermidine synthase
2.5.1.19 3-Phosphoshikimate-1-carboxyvinyl transferase
2.5.1.29 Farnesyltransferase
2.5.1.3 Thiamine phosphate pyrophosphorylase
2.5.1.31 Di-trans, poly-cis-decaprenylcistransferase
2.5.1.6 Methionine adenosyltransferase
2.5.1.7 UDP-N-Acetylglucosamine-1-carboxyvinyltransferase
2.5.1.9 Riboflavin synthase
2.6.1.1 Aspartate Transaminase
2.6.1.11 Acetylornithine transaminase
2.6.1.16 glutamine-fructose-6-phosphate transaminase (isomerizing)
2.6.1.19 4-aminobutyrate transaminase
2.6.1.21 D-alanine transaminase
2.6.1.42 branched chain amino acid transaminase
2.6.1.44 Alanine Glyoxylate Transaminase
2.6.1.45 Serine glyoxylate transaminase
2.6.1.51 Serine pyruvate transaminase
2.6.1.62 Adenosylmethionine-8-amino-7-oxononanoate transaminase
2.6.1.9 Histidinol phosphate transaminase
2.7.1.11 6-phosphofructokinase
2.7.1.17 xylulokinase
2.7.1.21 Thymidine kinase
2.7.1.25 Adenylyl sulfate kinase
2.7.1.30 glycerol kinase
2.7.1.37 protein kinase
2.7.1.39 Homoserine Kinase
2.7.1.4 Fructokinase
2.7.1.40 pyruvate kinase
2.7.1.49 hydroxymethylpyrimidine kinase
2.7.1.50 Hydroxyethylthiazole kinase
2.7.1.56 1-phosphofructokinase
2.7.1.6 Galactokinase
2.7.1.69 Protein-N (pai) -phosphohistidine-sugar-phosphotransferase
2.7.1.71 Shikimate kinase
2.7.2.1 acetate kinase
2.7.2.11 Glutamate 5-kinase
2.7.2.3 Phosphoglycerate kinase
2.7.2.4 Aspartate Kinase
2.7.2.7 Butyrate kinase
2.7.2.8 acetylglutamate kinase
2.7.3.3 Arginine Kinase
2.7.3.9 Phosphoenolpyruvate protein phosphotransferase
2.7.4.1 polyphosphate kinase
2.7.4.14 Cytidylate kinase
2.7.4.3 Adenylate kinase
2.7.4.8 guanylate kinase
2.7.6.1 Ribosephosphate Pyrophosphokinase
2.7.6.2 Thiamine pyrophosphokinase
2.7.6.3 2-Amino-4-hydroxy-6-hydroxymethyldihydropteridine pyro
2.7.6.5 GTP pyrophosphokinase
2.7.7.10 UTP hexose-1-phosphate uridylyl transferase
2.7.7.12 UDP-glucose-hexose-1-phosphate uridylyl transferase
2.7.7.13 Mannose 1-phosphate guanylyl transferase
2.7.7.19 Polynucleotide adenylyl transferase
2.7.7.24 Glucose-1-phosphate-thymidylyl transferase
2.7.7.25 tRNA adenylyl transferase
2.7.7.27 Glucose-1-phosphate adenylyl transferase
2.7.7.4 Sulfate adenylyl transferase
2.7.7.6 DNA-related RNA polymerase
2.7.7.7 DNA-related DNA polymerase
2.7.7.8 Polyribonucleotide Nucleotidyl Transferase
2.7.7.9 UTP-glucose-1-phosphate uridylyl transferase
2.7.8.13 Phospho-N-acetylmuramoylpentapeptide transferase
2.7.8.5 CDP-diacylglycerol-glycerol-3-phosphate-3-phosphatidyltransferase
2.7.8.6 Undecaprenyl phosphate galactose phosphotransferase
2.7.8.7 Holo [acyl carrier protein] synthase
2.7.8.8 CDP-diacylglycerol-serine-O-phosphatidyltransferase
2.7.9.2 Pyruvate water dikinase
3.1.1.11 pectin esterase
3.1.1.2 aryl esterase
3.1.1.29 aminoacyl tRNA hydrolase
3.1.1.3 triacylglycerol lipase
3.1.1.5 Lisophospholipase
3.1.1.61 protein glutamate methyl esterase
3.1.11.5 Exodesoxyribonuclease V
3.1.11.6 Exodesoxyribonuclease VII
3.1.2.14 Oleoyl- [Acyl Carrier Protein] hydrolase
3.1.2.6 Hydroxyacylglutathione hydrolase
3.1.21.4 Type II sequence-specific deoxyribonuclease
3.1.26.3 Ribonuclease III
3.1.26.4 calf thymus ribonuclease H
3.1.27.1 Ribonuclease T2
3.1.3.11 Fructose bisphosphatase
3.1.3.15 histidinol phosphatase
3.1.3.16 Phosphoprotein phosphatase
3.1.3.18 phosphoglycolate phosphatase
3.1.3.48 protein tyrosine phosphatase
3.1.3.5 5'-nucleotide
3.1.4.1 Phosphodiesterase I
3.1.4.14 [Acyl Carrier Protein] phosphodiesterase
3.1.4.16 2 ', 3'-cyclic nucleotide 2'-phosphodiesterase
3.1.4.3 phospholipase c
3.1.4.46 glycerophosphodiester phosphodiesterase
3.2.1.1 α-amylase
3.2.1.135 Neopullulanase
3.2.1.14 chitinase
3.2.1.17 Lysozyme
3.2.1.20 α-glucosidase
3.2.1.21 β-glucosidase
3.2.1.23 β-galactosidase
3.2.1.25 β-mannosidase
3.2.1.26 β-fructofuranosidase
3.2.1.37 xylan-1,4-β-xylosidase
3.2.1.39 Glucan-endo-1,3-β-D-glucosidase
3.2.1.4 Cellulase
3.2.1.41 α-dextrin-endo-1,6-alpha-glucosidase
3.2.1.52 β-N-acetylhexosaminidase
3.2.1.54 Cyclomaltodextrinase
3.2.1.55 α-N-arabinofuranosidase
3.2.1.58 Glucan-1,3-β-glucosidase
3.2.1.60 glucan-1,4-α-maltotetraohydrolase
3.2.1.65 Levanase
3.2.1.7 inulinase
3.2.1.73 Licheninase
3.2.1.74 Glucan-1,4-β-glucosidase
3.2.1.78 Mannan-endo-1,4-β-mannosidase
3.2.1.8 Endo-1,4-β-xylanase
3.2.1.82 Exo-poly-α-galacturonosidase
3.2.1.85 6-phospho-β-galactosidase
3.2.1.86 6-phospho-β-glucosidase
3.2.1.91 Cellulose-1,4-β-cellobiosidase
3.2.2.17 Deoxyribodipyrimidine donucleosidase
3.2.2.20 DNA-3-methyladenine glycosidaseI
3.2.2.24 ADP-Ribosyl- [Nitrogen Reductase] hydrolase
3.3.2.3 Epoxy hydrolase
3.4.13.9 X-Pro dipeptidase
3.4.16.4 Serine type D-Ala, D-Ala carboxypeptidase
3.4.21.53 Endopeptidase Ia
3.4.21.62 Subtilisin
3.4.23.33 Xanthomonapepsin
3.4.23.36 Signal peptidase II
3.4.24.27 Thermolysin
3.4.24.28 Bacillolysin
3.4.24.55 Pitrilysin
3.4.24.57 O-Syaloglycoprotein endopeptidase
3.4.99.36 lead peptidase
3.4.99.41 mitochondrial processing peptidase
3.5.1.1 Asparaginase
3.5.1.18 succinyl-diaminopimelate desuccinylase
3.5.1.25 N-acetylglucosamine-6-phosphate deacetylase
3.5.1.27 N-Formylmethionylaminoacyl-tRNA deformylase
3.5.1.28 N-acetylmuramoyl-L-alanine amidase
3.5.1.38 glutamine (asparagine) ase
3.5.2.3 Dihydroorotase
3.5.2.6 β-lactamase
3.5.3.1 Arginase
3.5.4.10 IMP cyclohydrolase
3.5.4.12 dCMP deaminase
3.5.4.16 GTP cyclohydrolase I
3.5.4.19 Phosphoribosyl AMP cyclohydrolase
3.5.4.2 Adenine Deaminase
3.5.4.23 Blasticidin-S-deaminase
3.5.4.25 GTP cyclohydrolase II
3.5.4.4 Adenosine Deaminase
3.5.4.5 cytidine deaminase
3.5.4.9 Methenyl tetrahydrofolate cyclohydrolase
3.6.1.17 bis (5'-nucleosyl) tetraphosphatase (asymmetric)
3.6.1.23 dUTP pyrophosphatase
3.6.1.3 Adenosine triphosphatase
3.6.1.31 Phosphoribosyl-ATP pyrophosphatase
3.6.1.34 H + -transporting ATP synthase
3.6.1.36 H + / K + exchanging ATPase
3.6.1.38 Ca2 + transporting ATPase
3.6.1.41 bis (5'-nucleosyl) tetraphosphatase (symmetrical)
3.6.1.7 Acylphosphatase
3.6.1.9 Nucleotide pyrophosphatase
3.8.1.3 Haloacetate dalogenase
4.1.1.11 Aspartate 1 decarboxylase
4.1.1.18 Lysine decarboxylase
4.1.1.19 arginine decarboxylase
4.1.1.20 diaminopimelate decarboxylase
4.1.1.21 phosphoribosylaminoimidazole carboxylase
4.1.1.23 Orotidine 5'-phosphate decarboxylase
4.1.1.48 Indole-3-glycerol phosphate synthase
4.1.1.5 Acetolactate decarboxylase
4.1.1.50 Adenosylmethionine decarboxylase
4.1.1.65 Phosphatidylserine decarboxylase
4.1.2.13 Fructose bisphosphate aldolase
4.1.2.14 2-Dehydro-3-deoxyphosphogluconate aldolase
4.1.2.15 2-Dehydro-3-deoxyphosphoheptonate aldolase
4.1.2.25 Dihydroneopterin aldolase
4.1.2.4 Deoxyribosephosphataldolase
4.1.3.12 2-isopropylmalate synthase
4.1.3.16 4-Hydroxy-2-oxoglutarate aldolase
4.1.3.18 Acetolactate synthase
4.1.3.21 Homocitrate synthase
4.1.3.27 Anthranilate synthase
4.2.1.1 carbonate dehydratase
4.2.1.10 3-dehydroquinate dehydratase
4.2.1.11 Phosphopyruvate hydratase
4.2.1.12 Phosphogluconate dehydratase
4.2.1.13 L-serine dehydratase
4.2.1.17 Enoyl-CoA hydratase
4.2.1.19 Imidazole glycerol phosphate dehydratase
4.2.1.2 Fumarate hydratase
4.2.1.20 tryptophan synthase
4.2.1.22 Cystathionin-β-synthase
4.2.1.24 Porphobilinogen synthase
4.2.1.3 Aconite hydratase
4.2.1.32 L (+) - tartrate dehydratase
4.2.1.33 3-isopropylmalate dehydratase
4.2.1.36 Homoaconitate hydratase
4.2.1.46 dTDP-glucose-4,6-dehydratase
4.2.1.51 Prephenate dehydratase
4.2.1.52 Dihydrodipicolinate synthase
4.2.1.7 Altronate dehydratase
4.2.1.74 long chain enoyl-CoA hydratase
4.2.1.75 Uroporphyrinogen III synthase
4.2.1.8 Mannonate dehydratase
4.2.1.9 Dihydroxy acid dehydratase
4.2.2.2 Pectate lyase
4.2.99.11 methylglyoxal synthase
4.2.99.18 DNA (apurinic or apyrimidine sequence) lyase
4.2.99.2 threonine synthase
4.2.99.8 cysteine synthase
4.3.1.1 Aspartate Ammonia Lyase
4.3.1.4 Formiminotetrahydrofolatecyclodeaminase
4.3.1.7 Ethanolamine ammonia lyase
4.3.1.8 Hydroxymethylbilane Synthase
4.3.2.1 Argininosuccinate Lyase
4.3.2.2 Adenyl succinate lyase
4.4.1.8 Cystathionin beta lyase
4.6.1.3 3-dehydroquinate synthase
4.6.1.4 Chorismate synthase
5.1.1.1 Alanine racemase
5.1.1.3 Glutamate racemase
5.1.1.7 Diaminopimarate epimerase
5.1.2.3 3-Hydroxybutyryl-CoA epimerase
5.1.3.1 Ribulosephosphate-3-epimerase
5.1.3.13 dTDP-4-dehydrorhamnose-3,5-epimerase
5.1.3.14 UDP-N-acetylglucosamine-2-epimerase
5.1.3.2 UDP-Glucose-4-epimerase
5.1.3.3 Aldose-1-epimerase
5.1.3.4 L-Ribulosephosphate-4-epimerase
5.2.1.8 Peptidyl prolyl isomerase
5.3.1.1 Triose phosphate isomerase
5.3.1.10 Glucosamine-6-phosphate isomerase
5.3.1.12 Glucuronate isomerase
5.3.1.16 N- (5'-Phospho-D-ribosylformimino) -5-amino-1-
5.3.1.24 Phosphoribosylanthranilate isomerase
5.3.1.4 L-arabinose isomerase
5.3.1.6 Ribose-5-phosphate epimerase
5.3.1.8 Mannose-6-phosphate isomerase
5.3.1.9 Glucose-6-phosphate isomerase
5.3.3.8 Dodecenoyl-CoA-Δ-isomerase
5.4.2.1 Phosphoglycerate mutase
5.4.2.6 β-phosphoglucomutase
5.4.2.7 Phosphopentomutase
5.4.3.8 Glutamate-1-semialdehyde-2,1-aminomutase
5.4.99.12 tRNA pseudouridine synthase I
5.4.99.5 Chorismate mutase
5.5.1.1 Muconate cycloisomerase
5.99.1.2 DNA topoisomerase
5.99.1.3 DNA topoisomerase (ATP hydrolyzing)
6.1.1.1 Tyrosine tRNA ligase
6.1.1.10 methionine tRNA ligase
6.1.1.11 Serine tRNA ligase
6.1.1.12 Aspartate tRNA ligase
6.1.1.14 glycine tRNA ligase
6.1.1.15 Proline tRNA ligase
6.1.1.16 cysteine tRNA ligase
6.1.1.17 glutamate tRNA ligase
6.1.1.19 arginine tRNA ligase
6.1.1.2 Tryptophan tRNA ligase
6.1.1.20 phenylalanine tRNA ligase
6.1.1.21 histidine tRNA ligase
6.1.1.22 Asparagine tRNA ligase
6.1.1.3 Threonine tRNA ligase
6.1.1.4 Leucine tRNA ligase
6.1.1.5 isoleucine tRNA ligase
6.1.1.6 Lysine tRNA ligase
6.1.1.7 alanine tRNA ligase
6.1.1.9 valine tRNA ligase
6.3.1.2 Glutamate ammonia ligase
6.3.2.1 Pantoat-β-alanine ligase
6.3.2.13 UDP-N-acetylmuramoylalnyl-D-glutamate-2,6-diaminopimelate ligase
6.3.2.15 UDP-N-acetylmuramoylalanyl-D-glutamyl-2,6-
6.3.2.17 tetrahydrofolyl polyglutamate synthase
6.3.2.2 Glutamate cysteine ligase
6.3.2.4 D-Alanine-D-Alanine Ligase
6.3.2.6 Phosphoribosylaminoimidazole succinocarboxyamide synthase
6.3.2.8 UDP-N-acetylmuramate alanine ligase
6.3.2.9 UDP-N-acetylmuramoylalanine-D-glutamate ligase
6.3.3.1 Phosphoribosyl-Formylglycinamidinecycloligase
6.3.3.2 5-Formyltetrahydrofolatecycloligase
6.3.3.3 Dethiobiotin synthase
6.3.4.13 Phosphoribosylamine glycine ligase
6.3.4.14 Biotin carboxylase
6.3.4.15 Biotin [acetyl-CoA carboxylase] ligase
6.3.4.2 CTP synthase
6.3.4.3 Format tetrahydrofolate ligase
6.3.4.4 Adenylosuccinate Synthase
6.3.4.5 argininosuccinate synthase
6.3.5.2 GMP synthase (glutamine hydrolyzing)
6.3.5.3 Phosphoribosylformylglycinamidine synthase
6.3.5.4 asparagine synthase (glutamine hydrolyzing)
6.3.5.5 Carbamoyl phosphate synthase (glutamine hydrolyzing)
6.4.1.1 Pyruvate carboxylase
6.4.1.2 Acetyl-CoA carboxylase
6.5.1.2 DNA ligase (NAD +). Enzymes from Saccharomyces cerevisiae with EC number 1.1.1.1 Alcohol dehydrogenase
1.1.1.100 3-oxoacyl- [acyl carrier protein] reductase
1.1.1.14 L-Iditol-2-dehydrogenase
1.1.1.145 3-β-hydroxy-A5 steroid dehydrogenase
1.1.1.179 D-xylose-1-dehydrogenase (NADP +)
1.1.1.188 Prostaglandin F synthase
1.1.1.195 Cinnamyl alcohol dehydrogenase
1.1.1.2 Alcohol Dehydrogenase (NADP +)
1.1.1.202 1,3-propanediol dehydrogenase
1.1.1.205 IMP dehydrogenase
1.1.1.21 aldehyde reductase
1.1.1.23 histidine dehydrogenase
1.1.1.244 methanol dehydrogenase
1.1.1.25 Shikimate 5-dehydrogenase
1.1.1.27 L-lactate dehydrogenase
1.1.1.29 Glycerate dehydrogenase
1.1.1.3 Homoserine Dehydrogenase
1.1.1.34 hydroxymethylglutaryl-CoA reductase (NADPH)
1.1.1.35 3-Hydroxyacyl-CoA dehydrogenase
1.1.1.36 Acetoacetyl-CoA reductase
1.1.1.37 malate dehydrogenase
1.1.1.38 Malate dehydrogenase (oxaloacetate decarboxylating)
1.1.1.40 Malate dehydrogenase (oxaloacetate decarboxylating) (NADP +)
1.1.1.41 isocitrate dehydrogenase (NAD +)
1.1.1.42 isocitrate dehydrogenase (NADP +)
1.1.1.44 phosphogluconate dehydrogenase (decarboxylating)
1.1.1.49 glucose-6-phosphate-1-dehydrogenase
1.1.1.57 Fructuronate reductase
1.1.1.62 Estradiol-17β-dehydrogenase
1.1.1.73 Octanol dehydrogenase
1.1.1.77 lactaldehyde reductase
1.1.1.8 Glycerol-3-phosphate dehydrogenase (NAD +)
1.1.1.81 hydroxypyruvate reductase
1.1.1.85 3-isopropylmalate dehydrogenase
1.1.1.86 keto acid reductoisomerase
1.1.1.88 hydroxymethylglutaryl-CoA reductase
1.1.1.9 D-xylulose reductase
1.1.1.91 Aryl alcohol dehydrogenase (NADP +)
1.1.1.95 Phosphoglycerate dehydrogenase
1.1.2.3 L-lactate dehydrogenase (cytochrome)
1.1.2.4 D-lactate dehydrogenase (cytochrome)
1.1.3.15 (S) -2-hydroxy acid oxidase
1.1.3.24 L-galactonolactone oxidase
1.1.3.8 L-gulonolactone oxidase
1.1.99.5 Glycerol-3-phosphate dehydrogenase
1.10.2.2 Ubiquinol cytochrome c reductase
1.10.3.1 catechol oxidase
1.10.3.2 Laccase
1.11.1.11 L-ascorbate peroxidase
1.11.1.12 Phospholipid hydroperoxide glutathione peroxidase
1.11.1.5 cytochrome c-peroxidase
1.11.1.6 Catalase
1.11.1.9 Glutathione peroxidase
1.12.1.2 Hydrogen dehydrogenase
1.13.11.32 2-nitropropane dioxygenase
1.13.11.42 Indolamine pyrrole-2,3-dioxygenase
1.13.11.6 3-hydroxyanthranilate-3,4-dioxygenase
1.14.11.14 6β-hydroxyhyoscyamine epoxidase
1.14.13.8 Dimethylaniline monooxygenase (N-oxide-forming)
1.14.99.5 Stearoyl-CoA desaturase
1.14.99.7 Squalene monooxygenase
1.15.1.1 Superoxide dismutase
1.17.4.1 Ribonucleoside Diphosphate Reductase
1.18.1.2 Ferredoxin NADP + reductase
1.18.99.1 hydrogenase
1.2.1.1 formaldehyde dehydrogenase (glutathione)
1.2.1.11 Aspartate semialdehyde dehydrogenase
1.2.1.12 Glyceraldehyde-3-phosphate dehydrogenase (phosphorylating)
1.2.1.16 succinate semialdehyde dehydrogenase (NAD (P) +)
1.2.1.2 Format dehydrogenase
1.2.1.3 Aldehyde Dehydrogenase (NAD +)
1.2.1.31 L-aminoadipate semialdehyde dehydrogenase
1.2.1.38 N-Acetyl-γ-glutamyl phosphate reductase
1.2.1.41 Glutamate 5-semialdehyde dehydrogenase
1.2.1.5 aldehyde dehydrogenase (NAD (P) +)
1.2.1.8 Betaine aldehyde dehydrogenase
1.2.4.1 pyruvate dehydrogenase (lipoamide)
1.2.4.2 Oxoglutarate dehydrogenase (lipoamide)
1.3.1.13 Prephenate Dehydrogenase (NADP +)
1.3.1.23 Cholestenone-5β-reductase
1.3.1.29 cis-1,2-dihydro-1,2-dihydroxynaphthalene dehydrogenase
1.3.1.34 2,4-dienoyl-CoA reductase (NADPH)
1.3.1.9 Enoyl- [Acyl Carrier Protein] reductase (NADH)
1.3.3.1 Dihydroorotate oxidase
1.3.3.3 Coproporphyrinogen Oxidase
1.3.3.4 Protoporphyrinogen oxidase
1.3.3.6 Acyl-CoA oxidase
1.3.5.1 succinate dehydrogenase (ubiquinone)
1.3.99.1 succinate dehydrogenase
1.4.1.13 Glutamate Synthase (NADPH)
1.4.1.14 Glutamate Synthase (NADH)
1.4.1.2 Glutamate dehydrogenase
1.4.1.4 Glutamate Dehydrogenase (NADP +)
1.4.3.16 L-aspartate oxidase
1.4.3.5 Pyridoxamine phosphate oxidase
1.4.4.2 Glycine dehydrogenase (decarboxylating)
1.4.7.1 glutamate synthase (ferredoxin)
1.5.1.10 saccharopin dehydrogenase (NADP +, forming L-glutamate)
1.5.1.12 1-pyrroline-5-carboxylate dehydrogenase
1.5.1.15 methylene tetrahydrofolate dehydrogenase (NAD +)
1.5.1.2 Pyrroline-5-carboxylate reductase
1.5.1.20 methylene tetrahydrofolate reductase (NADPH)
1.5.1.3 Dihydrofolate reductase
1.5.1.5 methylene tetrahydrofolate dehydrogenase (NADP +)
1.5.1.7 saccharopin dehydrogenase (NAD +, forming L-lysine)
1.5.1.8 saccharopin dehydrogenase (NADP +, forming L-lysine)
1.5.99.8 Proline dehydrogenase
1.6.2.2 Cytochrome b5 reductase
1.6.2.4 NADPH ferrihemoprotein reductase
1.6.4.2 Glutathione reductase (NADPH)
1.6.4.5 Thioredoxin Reductase (NADPH)
1.6.5.3 NADH dehydrogenase (ubiquinone)
1.6.5.5 NADPH: quinone reductase
1.6.6.1 Nitrate reductase (NADH)
1.6.99.1 NADPH dehydrogenase
1.7.99.5 5,10-methylene tetrahydrofolate reductase (fadh2)
1.7.99.7 Nitric oxide reductase
1.8.1.2 Sulfite reductase (NADPH)
1.8.1.4 Dihydrolipoamide dehydrogenase
1.8.7.1 sulfite reductase (ferredoxin)
1.8.99.4
1.9.3.1 Cytochrome c oxidase
2.1.1.107 Uroporphyrin-III c-methyltransferase
2.1.1.14 5-methyltetrahydropteroyl triglutamate homocysteine
2.1.1.17 Phosphatidylethanolamine-N-methyltransferase
2.1.1.41 24-sterol c-methyl transferase
2.1.1.45 thymidylate synthase
2.1.1.63 methylated-DNA-protein-cysteine-S-methyltransferase
2.1.1.64 3-Demethylubiquinone-9-3-O-methyltransferase
2.1.1.71 Phosphatidyl-N-methylethanolamine-N-methyltransferase
2.1.1.98 diphthine synthase
2.1.2.1 Glycine hydroxymethyl transferase
2.1.2.10 aminomethyl transferase
2.1.2.11 3-methyl-2-oxobutanoate hydroxymethyl transferase
2.1.2.2 Phosphoribosylglycinamide formyl transferase
2.1.2.3 Phosphoribosylaminoimidazole carboxamide formyl transferase
2.1.2.9 Methionyl tRNA formyl transferase
2.1.3.2 Aspartate carbamoyl transferase
2.1.3.3 ornithine carbamoyl transferase
2.2.1.1 Transketolase
2.2.1.2 Transaldolase
2.3.1.12 Dihydrolipoamide S-acetyltransferase
2.3.1.128 Ribosomal protein alanine N-acetyltransferase
2.3.1.15 Glycerol-3-phosphate-O-acyltransferase
2.3.1.16 Acetyl-CoA-C acyltransferase
2.3.1.18 Galactoside O-acetyltransferase
2.3.1.21 Carnitine O-palmitoyl transferase
2.3.1.31 Homoserine O-acetyltransferase
2.3.1.35 Glutamate N-acetyltransferase
2.3.1.37 5-aminolevulinate synthase
2.3.1.38 [Acyl Carrier Protein] -S-Acetyltransferase
2.3.1.39 [Acyl carrier protein] -S-malonyltransferase
2.3.1.41 3-oxoacyl- [acyl carrier protein] synthase
2.3.1.43 Phosphatidylcholine-sterol-O-acyltransferase
2.3.1.47 8-Amino-7-oxononanoate synthase
2.3.1.48 Histone acetyl transferase
2.3.1.50 Serine C palmitoyl transferase
2.3.1.51 1-Acylglycerol-3-phosphate-O-acyltransferase
2.3.1.6 Choline O-acetyltransferase
2.3.1.61 Dihydrolipoamide-S-succinyl transferase
2.3.1.7 Carnitine-O-acetyltransferase
2.3.1.84 Alcohol-O-acetyltransferase
2.3.1.85 fatty acid synthase
2.3.1.86 Fatty acid acyl-CoA synthase
2.3.1.9 Acetyl-CoA-C acetyltransferase
2.3.1.97 Glycyl peptide N-tetradecanoyl transferase
2.3.2.2 γ-glutamyl transferase
2.3.2.5 Glutaminyl peptide cyclotransferase
2.3.2.8 Arginyl transferase
2.4.1.1 Phosphorylase
2.4.1.109 Dolichyl phosphate mannose protein mannosyltransferase
2.4.1.11 Glycogen (starch) synthase
2.4.1.117 Dolichylphosphate-β-glucosyltransferase
2.4.1.119 Dolichyldiphosphooligosaccharide protein glycosyltransferase
2.4.1.131 Glycolipid-2-α-mannosyltransferase
2.4.1.15 α, α-trehalose phosphate synthase (UDP-forming)
2.4.1.16 chitin synthase
2.4.1.18 1,4-α-glucan branching enzyme
2.4.1.186 Glycogenin glucosyltransferase
2.4.1.25 4-α-glucanotransferase
2.4.1.34 1,3-β-glucan synthase
2.4.1.83 Dolichylphosphate-β-D-mannosyltransferase
2.4.2.1 Purine Nucleoside Phosphorylase
2.4.2.10 Orotate phosphoribosyl transferase
2.4.2.11 Nicotinate phosphoribosyl transferase
2.4.2.14 Amidophosphoribosyltransferase
2.4.2.17 ATP phosphoribosyl transferase
2.4.2.18 Anthranilate phosphoribosyl transferase
2.4.2.19 Nicotinate nucleotide pyrophosphorylase (carboxylating)
2.4.2.28 5'-methylthioadenosine phosphorylase
2.4.2.29 Queuin tRNA ribosyltransferase
2.4.2.7 Adenine phosphoribosyl transferase
2.4.2.9 uracil phosphoribosyl transferase
2.5.1.1 Dimethylallyltrantransferase
2.5.1.10 Geranyltran transferase
2.5.1.15 Dihydropteroate synthase
2.5.1.16 Spermidine synthase
2.5.1.18 Glutathione transferase
2.5.1.19 3-Phosphoshikimate-1-carboxyvinyl transferase
2.5.1.21 Farnesyl diphosphate farnesyl transferase
2.5.1.29 Farnesyltransferase
2.5.1.3 Thiamine phosphate pyrophosphorylase
2.5.1.31 Di-trans, poly-cis-decaprenylcistransferase
2.5.1.32 Geranylgeranyl diphosphate geranylgeranyl transferase
2.5.1.33 trans-pentaprenyltransferase
2.5.1.6 Methionine adenosyltransferase
2.5.1.8 tRNA isopentenyl transferase
2.5.1.9 Riboflavin synthase
2.6.1.1 Aspartate Transaminase
2.6.1.11 Acetylornithine transaminase
2.6.1.13 Ornithine Oxo Acid Transaminase
2.6.1.14 Asparagine Oxo Acid Transaminase
2.6.1.16 glutamine-fructose-6-phosphate transaminase (isomerizing)
2.6.1.19 4-aminobutyrate transaminase
2.6.1.2 Alanine Transaminase
2.6.1.36 L-lysine-6-transaminase
2.6.1.42 branched chain amino acid transaminase
2.6.1.45 Serine glyoxylate transaminase
2.6.1.52 phosphoserine transaminase
2.6.1.62 Adenosylmethionine-8-amino-7-oxononanoate transaminase
2.6.1.64 Glutamine phenylpyruvate transaminase
2.6.1.7 Kynurenine oxoglutarate transaminase
2.6.1.9 Histidinol phosphate transaminase
2.7.1.1 Hexokinase
2nd, 7.1.105 6-phosphofructo-2-kinase
2.7.1.109 [Hydroxymethylglutaryl-CoA reductase (NADPH)] - kinase
2.7.1.11 6-phosphofructokinase
2.7.1.112 Protein Tyrosine Kinase
2.7.1.115 [3-methyl-2-oxobutanoate dehydrogenase (lipoamide)] - kinase
2.7.1.117 Myosin light chain kinase
2.7.1.12 Gluconokinase
2.7.1.123 Ca2 + / Calmodulin-dependent protein kinase
2.7.1.128 [Acetyl-CoA carboxylase] kinase
2.7.1.129 Myosin heavy chain kinase
2.7.1.135 τ protein kinase
2.7.1.137 1-phosphatidylinositol 3-kinase
2.7.1.141 [RNA polymerase] subunit kinase
2.7.1.15 Ribokinase
2.7.1.17 xylulokinase
2.7.1.2 Glucokinase
2.7.1.20 adenosine kinase
2.7.1.25 Adenylyl sulfate kinase
2.7.1.30 glycerol kinase
2.7.1.32 choline kinase
2.7.1.35 pyridoxalkinase
2.7.1.36 Mevalonate kinase
2.7.1.37 protein kinase
2.7.1.38 Phosphorylase kinase
2.7.1.39 Homoserine Kinase
2.7.1.40 pyruvate kinase
2.7.1.47 D-Ribulokinase
2.7.1.48 uridine kinase
2.7.1.49 hydroxymethylpyrimidine kinase
2.7.1.50 Hydroxyethylthiazole kinase
2.7.1.6 Galactokinase
2.7.1.67 1-phosphatidylinositol 4-kinase
2.7.1.71 Shikimate kinase
2.7.1.99 [pyruvate dehydrogenase (lipoamide)] - kinase
2.7.2.11 Glutamate 5-kinase
2.7.2.3 Phosphoglycerate kinase
2.7.2.4 Aspartate Kinase
2.7.2.8 acetylglutamate kinase
2.7.4.10 Nucleoside triphosphate adenylate kinase
2.7.4.14 Cytidylate kinase
2.7.4.2 Phosphomevalonate kinase
2.7.4.3 Adenylate kinase
2.7.4.6 Nucleoside diphosphate kinase
2.7.4.8 guanylate kinase
2.7.4.9 DTMP kinase
2.7.6.1 Ribosephosphate pyrophosphokinase
2.7.6.2 Thiamine pyrophosphokinase
2.7.7.10 UTP hexose-1-phosphate uridylyl transferase
2.7.7.12 UDP-glucose-hexose-1-phosphate uridylyl transferase
2.7.7.13 Mannose 1-phosphate guanylyl transferase
2.7.7.14 ethanolamine phosphate cytidylyl transferase
2.7.7.15 Choline phosphate cytidylyl transferase
2.7.7.19 Polynucleotide adenylyl transferase
2.7.7.25 tRNA adenylyl transferase
2.7.7.4 Sulfate adenylyl transferase
2.7.7.41 Phosphatidate cytidylyl transferase
2.7.7.5 Sulfate Adenylyl Transferase (ADP)
2.7.7.50 mRNA guanylyl transferase
2.7.7.53 ATP adenylyl transferase
2.7.7.56 tRNA nucleotidyl transferase
2.7.7.6 DNA-related RNA polymerase
2.7.7.7 DNA-related DNA polymerase
2.7.7.9 UTP-glucose-1-phosphate uridylyl transferase
2.7.8.1 ethanolamine phosphotransferase
2.7.8.11 CDP-diacylglycerol-inositol-3-phosphatidyltransferase
2.7.8.15 UDP-N-acetylglucosamine dolichyl
2.7.8.19 UDP-glucose-glycoprotein-glucose phosphotransferase
2.7.8.2 Diacylglycerolcholine phosphotransferase
2.7.8.5 CDP-diacylglycerol-glycerol-3-phosphate-3-phosphatidyl transferase
2.7.8.8 CDP-Diacylglycerolserin-O-phosphatidyltransferase
2.8.1.1 Thiosulfate Sulfur Transferase
3.1.1.1 Carboxylesterase
3.1.1.13 sterolesterase
3.1.1.29 aminoacyl tRNA hydrolase
3.1.1.3 triacylglycerol lipase
3.1.1.45 carboxymethylene butenolidase
3.1.1.5 Lisophospholipase
3.1.13.4 Poly (A) specific ribonuclease
3.1.2.1 Acetyl-CoA hydrolase
3.1.2.15 Ubiquitinthiolesterase
3.1.2.2 Palmitoyl-CoA hydrolase
3.1.2.6 Hydroxyacylglutathione hydrolase
3.1.21.2 Deoxyribonuclease IV (phage T4-induced)
3.1.26.3 Ribonuclease III
3.1.26.4 calf thymus ribonuclease H
3.1.26.5 Ribonuclease P
3.1.27.1 Ribonuclease T2
3.1.3.1 alkaline phosphatase
3.1.3.11 Fructose bisphosphatase
3.1.3.12 Trehalose phosphatase
3.1.3.15 histidinol phosphatase
3.1.3.16 Phosphoprotein phosphatase
3.1.3.17 [phosphorylase] phosphatase
3.1.3.2 acid phosphatase
3.1.3.25 Myoinositol-1 (or 4) monophosphatase
3.1.3.3 Phosphoserine Phosphatase
3.1.3.43 [pyruvate dehydrogenase (lipoamide)] - phosphatase
3.1.3.46 Fructose-2,6-bisphosphate-2-phosphatase
3.1.3.48 protein tyrosine phosphatase
3.1.3.7 3 '(2'), 5'-bisphosphate nucleotidase
3.1.3.8 3-phytase
3.1.4.1 Phosphodiesterase I
3.1.4.11 1-phosphatidylinositol-4,5-bisphosphate phosphodiesterase
3.1.4.12 Sphingomyelin phosphodiesterase
3.1.4.17 3,5'-cyclic nucleotide phosphodiesterase
3.1.4.46 glycerophosphodiester phosphodiesterase
3.1.4.50 Glycoprotein phospholipase D
3.2.1.10 Oligo-1,6-glucosidase
3.2.1.113 mannosyloligosaccharide-1,2-α-mannosidase
3.2.1.14 chitinase
3.2.1.15 polygalacturonase
3.2.1.20 α-glucosidase
3.2.1.24 α-mannosidase
3.2.1.26 β-fructofuranosidase
3.2.1.28 α, α-trehalase
3.2.1.3 Glucan 1,4-α-glucosidase
3.2.1.33 Amylo-1,6-glucosidase
3.2.1.58 Glucan-1,3-β-glucosidase
3.2.1.65 Levanase
3.2.1.7 inulinase
3.2.1.73 Licheninase
3.2.1.8 Endo-1,4-beta-xylanase
3.2.2.20 DNA-3-methyladenine glycosidaseI
3.3.1.1 Adenosyl homocysteinase
3.3.2.6 Leukotriene A4 hydrolase
3.4.11.10 bacterial leucyl aminopeptidase
3.4.11.18 Methionyl aminopeptidase
3.4.11.2 Membrane alanyl aminopeptidase
3.4.11.7 Glutamyl aminopeptidase
3.4.11.9 X-pro aminopeptidase
3.4.13.9 X-Pro dipeptidase
3.4.14.11 XAA-Pro-Dipeptidylpeptidase
3.4.14.5 Dipeptidyl peptidase IV
3.4.16.1 Serine type carboxypepetidase
3.4.17.1 Carboxypeptidase A
3.4.17.15 Carboxypeptidase A2
3.4.17.2 Carboxypeptidase b
3.4.17.4 Gly-X carboxypeptidase
3.4.21.48 Cerevisin
3.4.21.53 Endopeptidase Ia
3.4.21.61 Kexin
3.4.22.17 Calpain
3.4.23.18 Aspergillopepsin I
3.4.23.24 Candidapepsin
3.4.23.25 saccharopepsin
3.4.23.5 Cathepsin D
3.4.24.15 Thimetoligopeptidase
3.4.24.37 Saccharolysin
3.4.24.57 O-Syaloglycoprotein endopeptidase
3.4.99.41 Mitochondrial processing peptidase
3.4.99.45 insulinase
3.4.99.46 Multicatalytic endopeptidase complex
3.5.1.1 Asparaginase
3.5.1.14 amino acylase
3.5.1.38 glutamine (asparagine) ase
3.5.1.4 Amidase
3.5.1.41 chitin deacetylase
3.5.2.14 N-methylhydantoinase (ATP hydrolyzing)
3.5.2.2 Dihydropyrimidinase
3.5.2.3 Dihydroorotase
3.5.2.5 Allantoinase
3.5.3.1 Arginase
3.5.3.19 Ureidoglycolate hydrolase
3.5.3.4 Allantoicase
3.5.4.10 IMP cyclohydrolase
3.5.4.12 dCMP deaminase
3.5.4.16 GTP cyclohydrolase I
3.5.4.19 Phosphoribosyl AMP cyclohydrolase
3.5.4.25 GTP cyclohydrolase II
3.5.4.4 Adenosine Deaminase
3.5.4.5 cytidine deaminase
3.5.4.6 AMP deaminase
3.5.4.9 Methenyl tetrahydrofolate cyclohydrolase
3.5.5.1 Nitrilase
3.6.1.1 inorganic pyrophosphatase
3.6.1.11 Exopolyphosphatase
3.6.1.17 bis (5'-nucleosyl) tetraphosphatase (asymmetric)
3.6.1.23 dUTP pyrophosphatase
3.6.1.3 Adenosine triphosphatase
3.6.1.31 Phosphoribosyl-ATP pyrophosphatase
3.6.1.32 Myosin ATPase
3.6.1.33 Dynein ATPase
3.6.1.34 H + -transporting ATP synthase
3.6.1.35 H + transporting ATPase
3.6.1.38 Ca2 + transporting ATPase
3.6.1.42 guanosine diphosphatase
3.6.1.5 Apyrase
3.6.1.9 Nucleotide pyrophosphatase
3.7.1.3 Kynureninase
4.1.1.1 Pyruvate decarboxylase
4.1.1.15 glutamate decarboxylase
4.1.1.17 ornithine decarboxylase
4.1.1.21 phosphoribosylaminoimidazole carboxylase
4.1.1.23 Orotidine 5'-phosphate decarboxylase
4.1.1.33 Diphosphomevalonate decarboxylase
4.1.1.37 uroporphyrinogen decarboxylase
4.1.1.48 Indole-3-glycerol phosphate synthase
4.1.1.49 Phosphoenolpyruvate Carboxykinase (ATP)
4.1.1.50 Adenosylmethionine decarboxylase
4.1.1.65 Phosphatidylserine decarboxylase
4.1.1.8 Oxalyl-CoA decarboxylase
4.1.2.13 Fructose bisphosphate aldolase
4.1.2.15 2-Dehydro-3-deoxyphosphoheptonate aldolase
4.1.2.25 Dihydroneopterin aldolase
4.1.3.1 Isocitrate lyase
4.1.3.12 2-isopropylmalate synthase
4.1.3.18 Acetolactate synthase
4.1.3.2 Malate synthase
4.1.3.27 Anthranilate synthase
4.1.3.5 Hydroxymethylglutaryl-CoA synthase
4.1.3.7 Citrate (SI) synthase
4.1.99.3 deoxyribodipyrimidine photolyase
4.2.1.1 carbonate dehydratase
4.2.1.10 3-dehydroquinate dehydratase
4.2.1.11 Phosphopyruvate hydratase
4.2.1.12 Phosphogluconate dehydratase
4.2.1.13 L-serine dehydratase
4.2.1.16 threonine dehydratase
4.2.1.17 Enoyl-CoA hydratase
4.2.1.19 Imidazole glycerol phosphate dehydratase
4.2.1.2 Fumarate hydratase
4.2.1.20 tryptophan synthase
4.2.1.22 Cystathionin-β-synthase
4.2.1.24 Porphobilinogen synthase
4.2.1.3 Aconite hydratase
4.2.1.33 3-isopropylmalate dehydratase
4.2.1.36 Homoaconitate hydratase
4.2.1.51 Prephenate dehydratase
4.2.1.61 3-hydroxypalmitoyl- [acyl carrier protein] dehydratase
4.2.1.69 cyanamide hydratase
4.2.1.75 Uroporphyrinogen III synthase
4.2.1.9 Dihydroxy acid dehydratase
4.2.99.18 DNA (apurinic or apyrimidine sequence) lyase
4.2.99.2 threonine synthase
4.2.99.8 cysteine synthase
4.2.99.9 O-succinyl homoserine (thiol) lyase
4.3.1.8 Hydroxymethylbilane Synthase
4.3.2.1 Argininosuccinate Lyase
4.3.2.2 Adenyl succinate lyase
4.4.1.1 Cystathionin γ-lyase
4.4.1.11 methionine γ-lyase
4.4.1.17 Holocytochrome c synthase
4.4.1.5 Lactoylglutathione lyase
4.4.1.8 Cystathionin-β-lyase
4.6.1.1 Adenylate cyclase
4.6.1.3 3-dehydroquinate synthase
4.6.1.4 Chorismate synthase
4.99.1.1 Ferrochelatase
5.1.3.1 Ribulosephosphate-3-epimerase
5.1.3.2 UDP-Glucose-4-epimerase
5.1.3.3 Aldose-1-epimerase
5.2.1.8 Peptidyl prolyl isomerase
5.3.1.1 Triose phosphate isomerase
5.3.1.24 Phosphoribosylanthranilate isomerase
5.3.1.4 L-arabinose isomerase
5.3.1.6 Ribose-5-phosphate epimerase
5.3.1.8 Mannose-6-phosphate isomerase
5.3.1.9 Glucose-6-phosphate isomerase
5.3.3.1 Steroid Δ isomerase
5.3.3.2lsopentenyldiphosphat-Δ-isomerase
5.3.4.1 Protein disulfide isomerase
5.4.2.1 Phosphoglycerate mutase
5.4.2.2 Phosphoglucomutase
5.4.2.3 Phosphoacetylglucosamine mutase
5.4.2.4 Bisphosphoglycerate mutase
5.4.2.8 Phosphomannomutase
5.4.99.12 tRNA pseudouridine synthase I
5.4.99.5 Chorismate mutase
5.4.99.7 Lanosterol synthase
5.4.99.8 Cycloartenolsynthase
5.5.1.4 Myoinositol-1-phosphate synthase
5.99.1.2 DNA topoisomerase
5.99.1.3 DNA topoisomerase (ATP hydrolyzing)
6.1.1.1 Tyrosine tRNA ligase
6.1.1.10 methionine tRNA ligase
6.1.1.11 Serine tRNA ligase
6.1.1.12 Aspartate tRNA ligase
6.1.1.14 glycine tRNA ligase
6.1.1.15 Proline tRNA ligase
6.1.1.16 cysteine tRNA ligase
6.1.1.17 glutamate tRNA ligase
6.1.1.18 glutamine tRNA ligase
6.1.1.19 arginine tRNA ligase
6.1.1.2 Tryptophan tRNA ligase
6.1.1.20 phenylalanine tRNA ligase
6.1.1.21 histidine tRNA ligase
6.1.1.22 Asparagine tRNA ligase
6.1.1.3 Threonine tRNA ligase
6.1.1.4 Leucine tRNA ligase
6.1.1.5 isoleucine tRNA ligase
6.1.1.6 Lysine tRNA ligase
6.1.1.7 alanine tRNA ligase
6.1.1.9 valine tRNA ligase
6.2.1.1 Acetate-CoA ligase
6.2.1.3 long chain fatty acid CoA ligase
6.2.1.4 succinate CoA ligase (GTP-forming)
6.3.1.2 Glutamate ammonia ligase
6.3.2.1 Pantoat-β-alanine ligase
6.3.2.12 Dihydrofolate synthase
6.3.2.17 tetrahydrofolyl polyglutamate synthase
6.3.2.19 Ubiquitin protein ligase
6.3.2.2 Glutamate cysteine ligase
6.3.2.25 Tublin tyrosine ligase
6.3.2.3 Glutathione synthase
6.3.2.6 Phosphoribosylaminoimidazole succinocarboxyamide synthase
6.3.3.1 Phosphoribosyl-Formylglycinamidinecycloligase
6.3.3.2 5-Formyltetrahydrofolatecycloligase
6.3.3.3.3 Dethiobiotin synthase
6.3.4.13 Phosphoribosylamine glycine ligase
6.3.4.14 Biotin carboxylase
6.3.4.15 biotin [acetyl-CoA carboxylase] ligase
6.3.4.16 Carbamoyl phosphate synthase (ammonia)
6.3.4.2 CTP synthase
6.3.4.3 Format tetrahydrofolate ligase
6.3.4.4 Adenylosuccinate Synthase
6.3.4.5 argininosuccinate synthase
6.3.4.6 urea carboxylase
6.3.5.2 GMP synthase (glutamine hydrolyzing)
6.3.5.3 Phosphoribosylformylglycinamidine synthase
6.3.5.4 asparagine synthase (glutamine hydrolyzing)
6.3.5.5 Carbamoyl phosphate synthase (glutamine hydrolyzing)
6.4.1.1 Pyruvate carboxylase
6.4.1.2 Acetyl-CoA carboxylase
6.5.1.1 DNA ligase (ATP)
6.5.1.4 RNA 3'-phosphate cyclase.

Bei den Proteinen, die in die Zellintegrität involviert sind und dadurch den Biokatalysator erhalten und/oder modifizieren sind besonders die Proteine der DNA- Replikation, der DNA-Reparatur, der Transkription, der Translation, der Zellteilung sowie der extrazellulären Matrix zu nennen. Dies sind insbesondere DNA und RNA Polymerasen, DNA und RNA Helicasen, DNA und RNA einzelstrangbindende Proteine, DNA und RNA Ligasen, Topoisomerasen und Gyrasen sowie Rekombinasen. Ebenso Translationsfaktoren wie Elongationsfaktoren z. B. EF-Tu, EF-Ts, EF-G, Initiationsfaktoren, Release Faktoren sowie ribosomale Proteine, tRNA Synthetasen, Chaperone und Chaperonine sowie Bestandteile der extrazellulären Matrix wie etwa das Antigen 84. For the proteins involved in cell integrity are and thereby receive the biocatalyst and / or especially the proteins of the DNA are modified Replication, DNA repair, transcription, the Translation, cell division and extracellular To call the matrix. These are especially DNA and RNA Polymerases, DNA and RNA helicases, DNA and RNA single-stranded proteins, DNA and RNA ligases, Topoisomerases and gyrases as well as recombinases. As well Translation factors such as elongation factors e.g. B. EF-Tu, EF-Ts, EF-G, initiation factors, release factors as well ribosomal proteins, tRNA synthetases, chaperones and Chaperonins and components of the extracellular matrix such as the antigen 84.

Bei den Proteinen der Signalerkennung und -weiterleitung sind besonders die Histidin-Kinasen und Response- Regulatoren der prokaryotischen Zweikomponentensysteme zu nennen, ebenso Proteinkinasen und Proteinphosphatasen. Bei den eukaryotischen Signaltransduktionswegen sind die Systeme zu nennen bei denen folgende Komponenten beteiligt sind: Inositoltriphosphat (IP3), Diacylglycerol (DAG), G- Proteine, MAP-Kinasen, Tyrosin-Kinasen, Janus-Kinasen, PhospholipaseA, PhospholipaseC, ProteinkinaseC, sowie Calcium abhängige Systeme wie Calcium-Calmodulin-Kinasen (CAM-Kinasen). The proteins of signal recognition and transmission are especially the histidine kinases and response Regulators of prokaryotic two-component systems too name, as well as protein kinases and protein phosphatases. at the eukaryotic signal transduction pathways are To name systems in which the following components are involved are: Inositol triphosphate (IP3), diacylglycerol (DAG), G- Proteins, MAP kinases, tyrosine kinases, Janus kinases, PhospholipaseA, PhospholipaseC, ProteinkinaseC, as well Calcium dependent systems such as calcium calmodulin kinases (CAM kinases).

Bei den Proteinen der prokaryotischen Genregulation sind besonders folgende Familien von Transkriptionsfaktoren zu nennen
Sigma 70, Sigma 70 ECF-Familie, Sigma 54, von Sigma 54 abgeleitete Typen, MarR-Typ, LacI-Typ, GntR-Typ, LysR-Typ, ArsR-Typ, DeoD-Typ, AraC-Typ, GerE-Typ, Crp-Typ, Xre-Typ, MerR-Typ, TeuR-Typ, AsnC-Typ, LexA-Typ, Hth6-Typ, Response- Regulatoren (zwei Konponentensysteme), Fur-Typ, Bgl- Antiterminations-Typen, iclR-Typ, GreA/B-Typ, Fe-abhängige Transkriptionsfaktoren, HrcA-Typ, Arg-Repressor-Typ. The following families of transcription factors are particularly worth mentioning for the proteins of prokaryotic gene regulation
Sigma 70, Sigma 70 ECF family, Sigma 54, types derived from Sigma 54, MarR type, LacI type, GntR type, LysR type, ArsR type, DeoD type, AraC type, GerE type, Crp type, Xre type, MerR type, TeuR type, AsnC type, LexA type, Hth6 type, response regulators (two component systems), fur type, Bgl anti-termination types, iclR type, GreA / B type, Fe-dependent transcription factors, HrcA type, Arg repressor type.

Bei den Proteinen der eukaryotischen Genregulation sind besonders folgende Transkriptionsfaktoren zu nennen Transkriptionsfaktoren mit basischen Domänen wie z. B. Leucin-Zipper Faktoren (bZIP), Helix-100p-helix Faktoren (bHLH), Helix-100p-helix/Leucin Zipper Faktoren (bHLH-ZIP), NF-1 Typ Faktoren, RF-X Typ Faktoren, bHSH Typ Faktoren. Transkriptionsfaktoren mit Zink-Finger Domänen wie z. B. Cys4 Zink-Finger, Cys2His2 Zink-Finger oder Cys6 Cystein-Zink Cluster. Transkriptionsfaktoren mit Helix- Turn-Helix Motiven wie z. B. Homeo-Box Proteine, Heat shock Faktoren oder die Tryptophan Cluster Familie. Desweiteren Transkriptionsfaktoren mit Beta-Faltblatt Strukturen, wie z.B die Faktoren der p53-Familie, MADS-Box Proteine oder TATA-bindenden Proteine. The proteins of the eukaryotic gene regulation are to mention especially the following transcription factors Transcription factors with basic domains such as z. B. Leucine-Zipper Factors (bZIP), Helix-100p-helix Factors (bHLH), Helix-100p-helix / Leucine Zipper factors (bHLH-ZIP), NF-1 type factors, RF-X type factors, bHSH type Factors. Transcription factors with zinc finger domains such as B. Cys4 zinc fingers, Cys2His2 zinc fingers or Cys6 Cysteine-zinc cluster. Transcription factors with helix Turn-Helix motifs such as B. Homeo-box proteins, heat shock Factors or the tryptophan cluster family. Furthermore Transcription factors with beta-sheet structures, such as e.g. the factors of the p53 family, MADS box proteins or TATA-binding proteins.

Bei den Proteinen mit Speicherfunktion sind besonders die Systeme für die Synthese von für Polyphosphaten und Speicherkohlenhydraten wie Glycogen oder Stärke zu nennen, ebenso Speicherproteine für anorganische Ionen wie z. B. Eisen. The proteins with a storage function are especially those Systems for the synthesis of polyphosphates and To name storage carbohydrates like glycogen or starch, also storage proteins for inorganic ions such. B. Iron.

Proteine mit Transportfunktion sind ABC Transporter, primäre, sekundäre und bindeproteinabhängige Transportsysteme sowie Proteine die eine erleichterte Diffusion vermitteln. Beispiele sind die bakteriellen Maltose/Trehalose Bindeproteine (malE), Multi Drug Resistance Proteine, Komponenten der PTS-Systeme zur Zuckeraufnahme (HPr, Enzym I, II und III) und Transportproteine die die Komponenten der extrazellulären Matrix sezernieren. Daneben alle Proteine die bei der Aufnahme oder der Sekretion von Proteinen und Peptiden beteiligt sind. Proteins with a transport function are ABC transporters, primary, secondary and binding protein dependent Transport systems as well as proteins which made it easier Mediate diffusion. Examples are bacterial Maltose / trehalose binding proteins (malE), multi drug Resistance proteins, components of the PTS systems for Sugar intake (HPr, enzyme I, II and III) and Transport proteins that are the components of the extracellular Secret the matrix. In addition, all proteins that are in the Uptake or secretion of proteins and peptides involved.

Proteine die bei der zellulären Biogenese beteiligt sind. Proteine, die am Stickstoffstoffwechsel beteiligt sind, wie etwa die Uridilyltransferase oder aber Proteine die an der Ionen Homeostase beteiligt sind. Proteins involved in cellular biogenesis. Proteins that are involved in nitrogen metabolism, such as such as the uridilyltransferase or proteins that are on the Ions homeostasis are involved.

Proteine, die als Zielproteine dienen können, sowie Proteine, die ein gegenüber dem Wildtyp geändertes Phosphorylierungsmuster aufweisen, können durch eine der in der Literatur beschriebenen Methoden aufgefunden, bzw. nachgewiesen werden. Hierzu bieten sich die in Wind, M. et al., Anal. Chem. 2001, 73, 29-35, die in Ahn, N. & Resing, K., Nature Biotechnology 2001, 19, 317-318, und die in Quadroni, M. & James, P., Proteomics in functional Genomics, 2000 (Birkhäuser Verlag, Basel, Schweiz, Hrsg. P Jolles und H. Jörnvall) beschriebenen Methoden an, mit der eine Phosphorylierung des Proteins erkannt werden kann, oder jede Methode zur Protein- oder DNA-Analyse, bei der der Aminosäureaustausch gegenüber dem Wildtyp-Protein nachweisbar ist. Proteins that can serve as target proteins, as well Proteins that are different from the wild type Phosphorylation patterns can by one of the in found methods described in the literature, or be detected. For this purpose, the in Wind, M. et al., Anal. Chem. 2001, 73, 29-35, which in Ahn, N. & Resing, K., Nature Biotechnology 2001, 19, 317-318, and those in Quadroni, M. & James, P., Proteomics in functional Genomics, 2000 (Birkhäuser Verlag, Basel, Switzerland, ed. P Jolles and H. Jörnvall) described methods with which phosphorylation of the protein can be recognized, or any method of protein or DNA analysis where the amino acid exchange for the wild-type protein is detectable.

Die Erfindung der oben genannten, unter Verwendung von Mikroorganismen, in denen wenigstens ein Protein in seinem Phosphorylierungszustand gegenüber dem natürlich in diesem Organismus vorkommenden Protein dauerhaft geändert ist. The invention of the above, using Microorganisms that contain at least one protein in its Phosphorylation state over that of course in this Organism occurring protein is permanently changed.

Bei der Arbeit an der vorliegenden Erfindung konnte festgestellt werden, daß Mikroorganismen, insbesondere coryneforme Bakterien und E.coli nach Veränderung des Phosphorylierungszustandes von Proteinen in verbesserter Weise Aminosäuren, insbesondere L-Lysin und L-Threonin, und Vitamine, insbesondere Pantothensäure produzieren. Could work on the present invention found that microorganisms, in particular coryneform bacteria and E.coli after changing the Phosphorylation state of proteins in improved Wise amino acids, especially L-lysine and L-threonine, and Produce vitamins, especially pantothenic acid.

Die betrachteten, in ihrer Phosphorylierbarkeit geänderten Proteine können durch Einbringen von Plasmiden, die die proteincodierenden Gene oder Genkonstrukte mit dem gewünschten Aminosäureaustausch tragen, in die Zielzellen eingeschleust werden. Dabei können die Gensequenzen nach dem Einbringen entweder weiterhin auf Plasmiden in der Zelle vorliegen, oder in das Chromosom des Mikroorganismus integriert werden. The considered, changed in their phosphorylability Proteins can be obtained by introducing plasmids that support the protein-coding genes or gene constructs with the carry the desired amino acid exchange into the target cells be smuggled in. The gene sequences can be either continue to introduce plasmids in the Cell, or in the chromosome of the microorganism to get integrated.

Anleitungen zur zielgerichteten Mutagenese und zum Einbringen von Plasmiden in die Mikroorganismen findet der Fachmann unter anderem bei Martin et al. (Bio/Technology 5, 137-146 (1987)), bei Guerrero et al. (Gene 138, 35-41 (1994)), Tsuchiya und Morinaga (Bio/Technology 6, 428-430 (1988)), bei Eikmanns et al. (Gene 102, 93-98 (1991)), in der Europäischen Patentschrift EPS 0 472 869, im US-Patent 4,601,893, bei Schwarzer und Pühler (Bio/Technology 9, 84-87 (1991), bei Remscheid et al. (Applied and Environmental Microbiology 60, 126-132 (1994)), bei LaBarre et al. (Journal of Bacteriology 175, 1001-1007 (1993)), in der Patentanmeldung WO 96/15246, bei Malumbres et al. (Gene 134, 15-24 (1993)), in der japanischen Offenlegungsschrift JP-A-10-229891, bei Jensen und Hammer (Biotechnology and Bioengineering 58, 191-195 (1998)), bei Makrides (Microbiological Reviews 60 : 512-538 (1996)) und in bekannten Lehrbüchern der Genetik und Molekularbiologie. Instructions for targeted mutagenesis and for The plasmids are introduced into the microorganisms Expert among others with Martin et al. (Bio / Technology 5, 137-146 (1987)), by Guerrero et al. (Gene 138, 35-41 (1994)), Tsuchiya and Morinaga (Bio / Technology 6, 428-430 (1988)), by Eikmanns et al. (Gene 102, 93-98 (1991)), in European patent specification EPS 0 472 869, in the US patent 4,601,893, by Schwarzer and Pühler (Bio / Technology 9, 84-87 (1991), by Remscheid et al. (Applied and Environmental Microbiology 60, 126-132 (1994)), by LaBarre et al. (Journal of Bacteriology 175, 1001-1007 (1993)), in which Patent application WO 96/15246, to Malumbres et al. (Gene 134, 15-24 (1993)), in Japanese JP-A-10-229891, published by Jensen and Hammer (Biotechnology and Bioengineering 58, 191-195 (1998)) Makrides (Microbiological Reviews 60: 512-538 (1996)) and in well-known textbooks of genetics and molecular biology.

Als Plasmide eignen sich solche, die in dem ausgewählten Mikroorganismus repliziert und exprimiert werden. Z. B. können für coryneforme Bakterien zahlreiche bekannte Plasmidvektoren wie z. B. pZ1 (Menkel et al., Applied and Environmental Microbiology (1989) 64 : 549-554), pEKEx1 (Eikmanns et al., Gene 102 : 93-98 (1991)) oder pHS2-1 (Sonnen et al., Gene 107 : 69-74 (1991)) verwendet werden, die auf den kryptischen Plasmiden pHM1519, pBL1 oder pGA1 beruhen. Andere Plasmidvektoren wie z. B. solche, die auf pCG4 (US-A 4,489,160), oder pNG2 (Serwold-Davis et al., FEMS Microbiology Letters 66, 119-124 (1990)), oder pAG1 (US-A 5,158,891) beruhen, können in gleicher Weise für coryneforme Bakterien verwendet werden. Suitable plasmids are those in the selected one Microorganism can be replicated and expressed. E.g. can be numerous known for coryneform bacteria Plasmid vectors such as e.g. B. pZ1 (Menkel et al., Applied and Environmental Microbiology (1989) 64: 549-554), pEKEx1 (Eikmanns et al., Gene 102: 93-98 (1991)) or pHS2-1 (Sonnen et al., Gene 107: 69-74 (1991)) can be used those on the cryptic plasmids pHM1519, pBL1 or pGA1 based. Other plasmid vectors such as e.g. B. those based on pCG4 (US-A 4,489,160), or pNG2 (Serwold-Davis et al., FEMS Microbiology Letters 66, 119-124 (1990)), or pAG1 (US-A 5,158,891) can be based in the same way for coryneform bacteria can be used.

In Enterobacteriaceae replizierbare Plasmidvektoren wie z. B. von pACYC184 abgeleitete Kloniervektoren (Bartolomé et al.; Gene 102, 75-78 (1991)), pTrc99A (Amann et al.; Gene 69 : 301-315 (1988)) oder pSC101-Derivate (Vocke und Bastia, Proceedings of the National Academy of Science USA 80 (21): 6557-6561 (1983)) können verwendet werden. Es ist ebenfalls möglich, Mutationen, die die Expression der jeweiligen Gene betreffen, durch Sequenzaustausch (Hamilton et al. (Journal of Bacteriology 174, 4617-4622 (1989)), Konjugation oder Transduktion in verschiedene Stämme zu überführen. Plasmids vectors replicable in Enterobacteriaceae such as z. B. cloning vectors derived from pACYC184 (Bartolomé et al .; Gene 102: 75-78 (1991)), pTrc99A (Amann et al .; Gene 69: 301-315 (1988)) or pSC101 derivatives (Vocke and Bastia, Proceedings of the National Academy of Science USA 80 (21): 6557-6561 (1983)) can be used. It is also possible mutations that affect the expression of the affect respective genes, by sequence exchange (Hamilton et al. (Journal of Bacteriology 174, 4617-4622 (1989)), Conjugation or transduction into different strains too convict.

Die erfindungsgemäß hergestellten Mikroorganismen können kontinuierlich oder diskontinuierlich im batch-Verfahren (Satzkultivierung) oder im fed batch (Zulaufverfahren) oder repeated fed batch Verfahren (repetitives Zulaufverfahren) zum Zwecke der Produktion der Feinchemikalien, insbesondere von Aminosäuren, Nukleosiden, Nukleotiden, Pigmenten, Antibiotika oder Vitaminen kultiviert werden. Für jede Zellart stehen verschiedene geeignete Kulturbedingungen zur Verfügung. Die verwendete Kulturmethode ist für die Erfindung nicht limitierend. Eine Zusammenfassung über bekannte Kultivierungsmethoden sind im Lehrbuch von Chmiel (Bioprozeßtechnik 1. Einführung in die Bioverfahrenstechnik (Gustav Fischer Verlag, Stuttgart, 1991)) oder im Lehrbuch von Storhas (Bioreaktoren und periphere Einrichtungen (Vieweg Verlag, Braunschweig/Wiesbaden, 1994)) beschrieben. The microorganisms produced according to the invention can continuously or discontinuously in a batch process (Set cultivation) or in fed batch (feed process) or repeated fed batch process (repetitive feed process) for the purpose of producing fine chemicals, in particular of amino acids, nucleosides, nucleotides, pigments, Antibiotics or vitamins are cultivated. For every Various suitable culture conditions are available for the cell type Available. The culture method used is for the Not limiting invention. A summary of well-known cultivation methods are in Chmiel's textbook (Bioprocess engineering 1. Introduction to bioprocess engineering (Gustav Fischer Verlag, Stuttgart, 1991)) or in the textbook from Storhas (bioreactors and peripheral facilities (Vieweg Verlag, Braunschweig / Wiesbaden, 1994)).

Das zu verwendende Kulturmedium muß in geeigneter Weise den Ansprüchen der jeweiligen Stämme genügen. Beschreibungen von Kulturmedien verschiedener Mikroorganismen sind im Handbuch "Manual of Methods for General Bacteriology" der American Society for Bacteriology (Washington D. C., USA, 1981) enthalten. Als Kohlenstoffquelle können Zucker und Kohlehydrate wie z. B. Glucose, Saccharose, Lactose, Fructose, Maltose, Melasse, Stärke und Cellulose, Öle und Fette wie z. B. Sojaöl, Sonnenblumenöl, Erdnußöl und Kokosfett, Fettsäuren wie z. B. Palmitinsäure, Stearinsäure und Linolsäure, Alkohole wie z. B. Glycerin und Ethanol und organische Säuren wie z. B. Essigsäure oder Aminosäuren wie Glutamin verwendet werden. Diese Stoffe können einzeln oder als Mischung verwendet werden. Bei der Verwendung photosynthetischer Bakterien kann Licht als Energiequelle verwendet werden. Als Stickstoffquelle können organische stickstoffhaltige Verbindungen wie Peptone, Hefeextrakt, Fleischextrakt, Malzextrakt, Maisquellwasser, Sojabohnenmehl und Harnstoff oder anorganische Verbindungen wie Ammoniumsulfat, Ammoniumchlorid, Ammoniumphosphat, Ammoniumcarbonat und Ammoniumnitrat verwendet werden. Die Stickstoffquellen können einzeln oder als Mischung verwendet werden. Als Phosphorquelle können Phosphorsäure, Kaliumdihydrogenphosphat oder Dikaliumhydrogenphosphat oder die entsprechenden Natrium-haltigen Salze verwendet werden. Das Kulturmedium muß außerdem Salze von Metallen enthalten wie z. B. Magnesiumsulfat oder Eisensulfat, die für das Wachstum notwendig sind. Schließlich können essentielle Wuchsstoffe wie Aminosäuren und Vitamine zusätzlich zu den oben genannten Stoffen eingesetzt werden. Dem Kulturmedium können überdies geeignete Vorstufen zugesetzt werden. Die genannten Einsatzstoffe können zur Kultur in Form eines einmaligen Ansatzes hinzugegeben oder in geeigneter Weise während der Kultivierung zugefüttert werden. The culture medium to be used must be in a suitable manner The requirements of the respective tribes meet. descriptions of culture media of various microorganisms are in "Manual of Methods for General Bacteriology" of the American Society for Bacteriology (Washington DC, USA, 1981) included. Sugar and Carbohydrates such as B. glucose, sucrose, lactose, Fructose, maltose, molasses, starch and cellulose, oils and Fats such as B. soybean oil, sunflower oil, peanut oil and Coconut fat, fatty acids such as B. palmitic acid, stearic acid and linoleic acid, alcohols such as e.g. B. glycerin and ethanol and organic acids such as B. acetic acid or amino acids such as Glutamine can be used. These substances can be used individually or can be used as a mixture. When using Photosynthetic bacteria can use light as an energy source be used. Organic nitrogen-containing compounds such as peptones, yeast extract, Meat extract, malt extract, corn steep liquor, Soybean meal and urea or inorganic compounds such as ammonium sulfate, ammonium chloride, ammonium phosphate, Ammonium carbonate and ammonium nitrate can be used. The Nitrogen sources can be used individually or as a mixture be used. Phosphoric acid, Potassium dihydrogen phosphate or dipotassium hydrogen phosphate or the corresponding sodium-containing salts are used. The culture medium must also contain salts of metals such as B. magnesium sulfate or iron sulfate, for the Growth are necessary. After all, essentials Growth substances such as amino acids and vitamins in addition to above substances are used. The culture medium Suitable precursors can also be added. The Feedstocks mentioned can be used for culture in the form of a one-off approach or appropriately to be fed during cultivation.

Zur pH-Kontrolle der Kultur werden basische Verbindungen wie Natriumhydroxid, Kaliumhydroxid, Ammoniak bzw. Ammoniakwasser oder saure Verbindungen wie Phosphorsäure oder Schwefelsäure in geeigneter Weise eingesetzt. Zur Kontrolle der Schaumentwicklung können Antischaummittel wie z. B. Fettsäurepolyglykolester eingesetzt werden. Zur Aufrechterhaltung der Stabilität von Plasmiden können dem Medium geeignete selektiv wirkende Stoffe wie z. B. Antibiotika hinzugefügt werden. Um aerobe Bedingungen aufrechtzuerhalten, werden Sauerstoff oder Sauerstoffhaltige Gasmischungen wie z. B. Luft in die Kultur eingetragen. Die Temperatur der Kultur liegt normalerweise bei 20°C bis 45°C und vorzugsweise bei 25°C bis 40°C. Basic compounds are used to control the pH of the culture such as sodium hydroxide, potassium hydroxide, ammonia or Ammonia water or acidic compounds such as phosphoric acid or sulfuric acid used in a suitable manner. to Antifoam agents such as z. B. fatty acid polyglycol esters. to Maintaining the stability of plasmids can do that Medium suitable selectively acting substances such. B. Antibiotics are added. To aerobic conditions will maintain oxygen or Oxygen-containing gas mixtures such as B. Air into culture entered. The temperature of the culture is usually at 20 ° C to 45 ° C and preferably at 25 ° C to 40 ° C.

Die Analyse der hergestellten Feinchemikalien kann z. B. durch Anionenaustauschchromatographie mit anschließender Ninhydrin Derivatisierung erfolgen, so wie bei Spackman et al. (Analytical Chemistry, 30, (1958), 1190) beschrieben. The analysis of the fine chemicals produced can e.g. B. by anion exchange chromatography with subsequent Ninhydrin derivatization take place, as in Spackman et al. (Analytical Chemistry, 30, (1958), 1190).

Das erfindungsgemäße Verfahren dient zur fermentativen Herstellung von Feinchemikalien, insbesondere Aminosäuren, Nukleosiden, Nukleotiden und Vitaminen. Besonders bevorzugt hergestellte Feinchemikalien sind L-Lysin, L-Threonin, L- Methionin, L-Tryptophan und Pantothensäure. The method according to the invention is used for fermentative purposes Manufacture of fine chemicals, especially amino acids, Nucleosides, nucleotides and vitamins. Particularly preferred manufactured fine chemicals are L-lysine, L-threonine, L- Methionine, L-tryptophan and pantothenic acid.

BeispieleExamples

Die vorliegende Erfindung wird im folgenden anhand von Ausführungsbeispielen näher erläutert. The present invention will hereinafter be described with reference to Embodiments explained in more detail.

Beispiel 1example 1 Detektion von phosphorylierten Proteinen mit radioaktiver Markierung oder AntikörpernDetection of phosphorylated proteins with radioactive Labeling or antibodies

Zur Detektion phosphorylierter Proteine wurden zunächst Zellen aus Schüttelkolbenversuchen oder Fermentationen zu bestimmten Zeitpunkten oder Bedingungen gezogen. Zellen von Corynebacterium glutamicum wurden dann wie bei Hermann et al. (Electrophoresis 19, 3217-3221, 1998), Hermann et al. (Electrophoresis 21, 654-659, 2000) und Hermann et al. (Electrophoresis 22, 1712-1723, 2001) beschrieben aufgeschlossen und die Proteine aufgereinigt und fraktioniert. Die entsprechende Aufreinigung von Proteinen aus Zellkulturen mit Escherichia coli geschah wie bei Molloy et al. (Electrophoresis 19, 837-844, 1998) beschrieben. Die Proteine wurden dann im Falle von Corynebacterium glutamicum wie bei Hermann et al. (Electrophoresis 22, 1712-1723, 2001), im Falle von Escherichia coli wie bei Molloy et al. (Electrophoresis 19, 837-844, 1998) beschrieben mit Hilfe der zweidimensionalen Gelelektrophorese nach ihrem isoelektrischen Punkt und ihrem Molekulargewicht aufgetrennt. Nach Beendigung der Elektrophorese wurden die Proteine mit Hilfe des Semi-dry Blotting Verfahrens auf eine PVDF-Membran (Immobilon-P, Porendurchmesser 0,45 µm, Millipore, Eschborn, D) geblottet. Dazu wurde das Gel zunächst für 30 Minuten im Anodenpuffer equilibriert der aus 20 mM Tris und 150 mM Glycin, pH 8,3 bestand. Anschliessend wurden sechs Lagen Filterpapier (Novablot PKG/500, Pharmacia Biotech, Freiburg, D) in Anodenpuffer getränkt und auf die Anode des Blotting Gerätes (Multiphor II und Nova Blot, Pharmacia Biotech, Freiburg, D) gelegt. Darauf wurde die PVDF Membran gelegt und auf diese wiederum das zu blottende Gel. Danach wurden sechs Lagen Filterpapier in Kathodenpuffer getränkt, der zum Anodenpuffer noch zusätzlich 0,1% SDS enthielt, und die Lagen auf das Gel gelegt. Abschliessend wurde dann die Graphitkathode von oben aufgelegt. Der Transfer der Proteine erfolgte bei Raumtemperatur für ca. eine bis zwei Stunden bei 10 V, 0,8 mA/cm2 und 5 W. Nach dem Blotten wurde die Membran getrocknet. Im Anschluss wurde die Membran in einer Blocking Lösung aus 1% Rinderserumalbumin (Fraktion V, Sigma, Deisenhofen, D), 1% Polyvinylpyrrolidon (Sigma, Deisenhofen, D), 1% PEG 3350 (Sigma, Deisenhofen, D) und 0,2% Tween 20 (Sigma, Deisenhofen, D) in PBS Puffer gelöst. PBS Puffer bestand aus 274 mM NaCl, 5,4 mM KCl, 20 mM Na2HPO4 und 3,6 mM KH2PO4 bei einem pH von 7,2. Dann wurde die Membran für 1 Stunde in 12,5 ml einer Lösung des primären Antikörpers inkubiert. Der primäre Antikörper ist gegen phosphorylierte Aminosäuren gerichtet, insbesondere Phosphothreonin, Phosphoserin und Phosphotyrosin. Es wurden folgende Antikörper verwendet: monoklonale Anti-Phosphoserin Antikörper Klone 1C8, 4A3, 4A9, 4H4, 7F12 und 16B4; monoklonale Anti-Phosphothreonin Antikörper Klon 14B3, 1E11 und 4D11; monoklonale Anti-Phosphotyrosin Antikörper 4G10 und 3B12 (Biomol, Hamburg, D). Zur Inkubation der Membran wurden 12,5 µg des jeweiligen primären Antikörpers in 1 ml bidestilliertem Wasser gelöst und mit 12,5 ml Blocking Lösung aufgefüllt. Anschliessend wurde die Membran fünfmal für fünf Minuten in einer Waschlösung gewaschen, die aus 10 mM Tris (Sigma, Deisenhofen, D), 150 mM NaCl und 0,1% (v/v) Tween 20 (Sigma, Deisenhofen, D) bestand und mit HCl auf pH 7,4 eingestellt wurde. Dann wird die gewaschene Membran für eine Stunde in 100 ml Lösung des sekundären Antikörpers inkubiert. Dazu wurde je nach Art des primären Antikörpers 0,6 mg AffiniPure Ziege Anti Maus IgG oder IgM als alkalisches Phosphatase Konjugat (Dianova, Hamburg, D) in 1 ml bidestilliertem Wasser gelöst und 1 : 5000 bis 1 : 50 000 in bidestilliertem Wasser verdünnt. Dann wurde die Membran wiederum fünfmal für fünf Minuten in einer Waschlösung gewaschen, die aus 10 mM Tris (Sigma, Deisenhofen, D), 150 mM NaCl und 0,1% (v/v) Tween 20 (Sigma, Deisenhofen, D) bestand und mit HCl auf pH 7,4 eingestellt wurde. Abschliessend wurde die Membran mit 10 ml Detektionslösung behandelt bis eine Violettfärbung die Proteinspots oder eine Positivkontrolle anzeigt. Die dazu eingesetzte Detektionslösung bestand aus SigmaFast BCIP/NBT Buffered Substrate Tabletten (Sigma, Deisenhofen, D), von denen je eine in 10 ml bidestilliertem Wasser aufgelöst wurde. Nach der Färbung der Membran wurde diese mit bidestilliertem Wasser gewaschen, getrocknet und lichtgeschützt gelagert. Violett-gefärbte Proteinspots zeigen phosphorylierte Proteine an. Durch Molekulargewichtsstandards und Proteinmuster wurde das Spot-Muster auf den Membranen mit dem von Coomassie- gefärbten zweidimensionalen Gelen (Neuhoff et al., Electrophoresis 9, 255-262, 1988) verglichen und in Deckung gebracht. To detect phosphorylated proteins, cells were first drawn from shake flask experiments or fermentations at specific times or conditions. Corynebacterium glutamicum cells were then as described in Hermann et al. (Electrophoresis 19, 3217-3221, 1998), Hermann et al. (Electrophoresis 21, 654-659, 2000) and Hermann et al. (Electrophoresis 22, 1712-1723, 2001) and the proteins are purified and fractionated. The corresponding purification of proteins from cell cultures with Escherichia coli was carried out as in Molloy et al. (Electrophoresis 19, 837-844, 1998). The proteins were then in the case of Corynebacterium glutamicum as in Hermann et al. (Electrophoresis 22, 1712-1723, 2001), in the case of Escherichia coli as in Molloy et al. (Electrophoresis 19, 837-844, 1998) described using the two-dimensional gel electrophoresis separated according to their isoelectric point and their molecular weight. After the electrophoresis had ended, the proteins were blotted onto a PVDF membrane (Immobilon-P, pore diameter 0.45 μm, Millipore, Eschborn, D) using the semi-dry blotting method. For this purpose, the gel was first equilibrated for 30 minutes in the anode buffer which consisted of 20 mM Tris and 150 mM glycine, pH 8.3. Six layers of filter paper (Novablot PKG / 500, Pharmacia Biotech, Freiburg, D) were then soaked in anode buffer and placed on the anode of the blotting device (Multiphor II and Nova Blot, Pharmacia Biotech, Freiburg, D). The PVDF membrane was placed on top of it and the gel to be blotted on it. Then six layers of filter paper were soaked in cathode buffer, which also contained 0.1% SDS in addition to the anode buffer, and the layers were placed on the gel. The graphite cathode was then placed on top. The proteins were transferred at room temperature for about one to two hours at 10 V, 0.8 mA / cm 2 and 5 W. After blotting, the membrane was dried. The membrane was then blocked in a blocking solution of 1% bovine serum albumin (fraction V, Sigma, Deisenhofen, D), 1% polyvinylpyrrolidone (Sigma, Deisenhofen, D), 1% PEG 3350 (Sigma, Deisenhofen, D) and 0.2 % Tween 20 (Sigma, Deisenhofen, D) dissolved in PBS buffer. PBS buffer consisted of 274 mM NaCl, 5.4 mM KCl, 20 mM Na 2 HPO 4 and 3.6 mM KH 2 PO 4 at a pH of 7.2. Then the membrane was incubated for 1 hour in 12.5 ml of a solution of the primary antibody. The primary antibody is directed against phosphorylated amino acids, especially phosphothreonine, phosphoserine and phosphotyrosine. The following antibodies were used: monoclonal anti-phosphoserine antibodies clones 1C8, 4A3, 4A9, 4H4, 7F12 and 16B4; monoclonal anti-phosphothreonine antibodies clone 14B3, 1E11 and 4D11; monoclonal anti-phosphotyrosine antibodies 4G10 and 3B12 (Biomol, Hamburg, D). To incubate the membrane, 12.5 µg of the respective primary antibody was dissolved in 1 ml of double-distilled water and filled with 12.5 ml of blocking solution. The membrane was then washed five times for five minutes in a washing solution consisting of 10 mM Tris (Sigma, Deisenhofen, D), 150 mM NaCl and 0.1% (v / v) Tween 20 (Sigma, Deisenhofen, D) and was adjusted to pH 7.4 with HCl. The washed membrane is then incubated for one hour in 100 ml of the secondary antibody solution. Depending on the type of primary antibody, 0.6 mg AffiniPure Goat Anti Mouse IgG or IgM as alkaline phosphatase conjugate (Dianova, Hamburg, D) was dissolved in 1 ml of double-distilled water and diluted 1: 5000 to 1: 50,000 in double-distilled water. Then the membrane was washed again five times for five minutes in a washing solution consisting of 10 mM Tris (Sigma, Deisenhofen, D), 150 mM NaCl and 0.1% (v / v) Tween 20 (Sigma, Deisenhofen, D) and adjusted to pH 7.4 with HCl. Finally, the membrane was treated with 10 ml detection solution until a violet color indicates the protein spots or a positive control. The detection solution used consisted of SigmaFast BCIP / NBT Buffered Substrate tablets (Sigma, Deisenhofen, D), each of which was dissolved in 10 ml of double-distilled water. After the membrane had been colored, it was washed with bidistilled water, dried and stored away from light. Violet-colored protein spots indicate phosphorylated proteins. The spot pattern on the membranes was compared with that of Coomassie-stained two-dimensional gels (Neuhoff et al., Electrophoresis 9, 255-262, 1988) and matched using molecular weight standards and protein patterns.

Neben der hier beschriebenen Detektion über Antikörper kann jede andere Methode zur Detektion phosphorylierter Proteine eingesetzt werden. Bedeutsam ist vor allem die Markierung der Proteine mit 32P oder 33P, bei der wachsenden Zellen radioaktives 32PO4 3- oder 33PO4 3- zugegeben wird. Mit Hilfe der Autoradiographie werden dann phosphorylierte Proteine identifiziert (Gooley und Packer, in: Proteome Research: New Frontiers in Functional Genomics, Springer Verlag, Berlin, 65-92, 1997). In addition to the detection via antibodies described here, any other method for the detection of phosphorylated proteins can be used. Of particular importance is the labeling of the proteins with 32 P or 33 P, in which growing cells are added radioactive 32 PO 4 3- or 33 PO 4 3- . Using autoradiography, phosphorylated proteins are then identified (Gooley and Packer, in: Proteome Research: New Frontiers in Functional Genomics, Springer Verlag, Berlin, 65-92, 1997).

Die zu untersuchenden Proteine können neben der zweidimensionalaen Gelelektrophorese auch mit jeder anderen Trennmethode isoliert werden, so z. B. Kapillarelektrophorese (Liu et al., Journal of Chromatography A, 918 (2), 401-409, 2001), alle Arten von chromatographischen Trennmethoden einschliesslich der Dünnschichtchromatographie oder aber durch Anwendung molekularbiologischer Methoden wie etwa der His-tagging Technologie. The proteins to be examined can in addition to the two-dimensional gel electrophoresis with any other Separation method are isolated, such. B. Capillary electrophoresis (Liu et al., Journal of Chromatography A, 918 (2), 401-409, 2001), all types of chromatographic separation methods including Thin layer chromatography or by application molecular biological methods such as His tagging Technology.

Die Identifizierung der phosphorylierten Proteine erfolgte wie bei Hermann et al. (Electrophoresis 22, 1712-1723, 2001) beschrieben durch Ausschneiden der Spots aus Coomassie gefärbten Gelen, Verdau der Proteine mit spezifischen Proteasen und anschliessender Identifikation der Peptide mittels MALDI oder Elektrospray Massenspektrometrie. The phosphorylated proteins were identified as in Hermann et al. (Electrophoresis 22, 1712-1723, 2001) described by cutting out the spots Coomassie stained gels, using proteins to digest them specific proteases and subsequent identification the peptides using MALDI or electrospray Mass spectrometry.

Beispiel 2Example 2 Detektion von Phosphorylierungsstellen in bakteriellen Proteinen mit ESI-MS und ICP-MSDetection of phosphorylation sites in bacterial Proteins with ESI-MS and ICP-MS

Phosphorylierungsstellen können in reinen Proteinen, Polypeptiden oder Proteingemischen wie bei Neubauer und Mann (Analytical Chemistry, 71(1), 235-242, 1999), Yan et al. (Journal of Chromatography A, 808(1-2), 23-41, 1998), Oda et al. (Proceedings of the National Academy of Sciences of the USA, 96, 6591-6596, 1999), Wind et al. (Anal. Chem. 73, 29-35, 2001), Ahn und Resing (Nature Biotechnology 19, 317-318, 2001) oder Quadroni und James (in: Proteomics in Functional Genomics, irkhäuser Verlag, Basel, Schweiz, Hrsg. P Jollès und H. Jörnvall, 2000) identifiziert werden. Eine Übersicht über Methoden zur Identifizierung von Phosphroylierungsstellen in Proteinen oder Peptiden findet sich weiterhin bei Gooley und Packer (in: Proteome Research: New Frontiers in functional Genomics, Springer Verlag, Berlin, 65-92, 1997). Phosphorylation sites can be found in pure proteins, Polypeptides or protein mixtures as with Neubauer and Mann (Analytical Chemistry, 71 (1), 235-242, 1999), Yan et al. (Journal of Chromatography A, 808 (1-2), 23-41, 1998), Oda et al. (Proceedings of the National Academy of Sciences of the USA, 96, 6591-6596, 1999), Wind et al. (Anal. Chem. 73, 29-35, 2001), Ahn and Resing (Nature Biotechnology 19, 317-318, 2001) or Quadroni and James (in: Proteomics in Functional Genomics, irkhäuser Verlag, Basel, Switzerland, ed. P Jollès and H. Jörnvall, 2000) be identified. An overview of methods for Identification of phosphroylation sites in proteins or peptides can still be found at Gooley and Packer (in: Proteome Research: New Frontiers in functional Genomics, Springer Verlag, Berlin, 65-92, 1997).

Beispiel 3Example 3 Modifikation der Phosphorylierungsstelle in der Enolase aus Corynebakterium glutamicum durch site directed mutagenesisModification of the phosphorylation site in the enolase Corynebacterium glutamicum by site directed mutagenesis 3.1. Gewinnung der für eno kodierenden DNA3.1. Obtaining the DNA coding for eno

Der Corynebacterium glutamicum Stamm ATCC13032 wird als Donor für die chromosomale DNA verwendet. Aus dem Stamm ATCC13032 wird mit den üblichen Methoden (Eikmanns et al., Microbiology 140: 1817-1828 (1994)) chromosomale DNA isoliert. Mit Hilfe der Polymerase-Kettenreaktion wird ein DNA-Fragment amplifiziert, welches das eno-Gen trägt. Aufgrund der für C. glutamicum bekannten Sequenz des eno- Geris (SEQ ID No. 1) (Accession number AX136862) werden folgende Primer-Oligonukleotide für die PCR ausgewählt:
6H-eno1 (SEQ ID No. 7):
5' CGC(GGA TCC)GCT GAA ATC ATG CAC GTA TTC 3'
6H-eno2 (SEQ ID No. 8):
5' GCG(AGA TCT)(CCC GGG)TTA GCC CTG AAA GCG TGG C 3'. The Corynebacterium glutamicum strain ATCC13032 is used as a donor for the chromosomal DNA. Chromosomal DNA is isolated from the ATCC13032 strain using the customary methods (Eikmanns et al., Microbiology 140: 1817-1828 (1994)). With the help of the polymerase chain reaction, a DNA fragment is carried which carries the eno gene. The following primer oligonucleotides are selected for the PCR based on the sequence of the eno-geris (SEQ ID No. 1) (accession number AX136862) known for C. glutamicum:
6H-eno1 (SEQ ID No. 7):
5 'CGC (GGA TCC) GCT GAA ATC ATG CAC GTA TTC 3'
6H-eno2 (SEQ ID No. 8):
5 'GCG (AGA TCT) (CCC GGG) TTA GCC CTG AAA GCG TGG C 3'.

Die dargestellten Primer werden von der Firma MWG Biotech synthetisiert und nach der Standard-PCR-Methode von Innis et al. (PCR protocols. A guide to methods and applications, 1990, Academic Press) die PCR Reaktion durchgeführt. Die Primer ermöglichen die Amplifizierung eines ca. 1,3 kb großen DNA-Fragmentes, welches das eno-Gen trägt. Außerdem enthalten die Primer die Sequenz für Schnittstellen der Restriktionsendonukleasen BamHI (6H-eno1) bzw. XmaI und BglII(6H-eno2), die in der oben dargestellten Nukleotidabfolge durch Klammern markiert sind. The primers shown are from MWG Biotech synthesized and according to the standard PCR method from Innis et al. (PCR protocols. A guide to methods and applications, 1990, Academic Press) carried out the PCR reaction. The Primers allow the amplification of an approximately 1.3 kb large DNA fragment that carries the eno gene. Moreover the primers contain the sequence for interfaces of the Restriction endonucleases BamHI (6H-eno1) or XmaI and BglII (6H-eno2) shown in the above Sequences of nucleotides are marked by brackets.

Das amplifizierte DNA Fragment von ca. 1,3 kb, welches das eno-Gen trägt, wird über Gelelektrophorese in einem 0,8%igen Agarosegel identifiziert und mit den üblichen Methoden aufgereinigt (High Pure PCR Product Purification Kit, Roche Diagnostics GmbH, Mannheim). The amplified DNA fragment of approximately 1.3 kb, which the eno gene is carried out via gel electrophoresis in one 0.8% agarose gel identified and with the usual Methods purified (High Pure PCR Product Purification Kit, Roche Diagnostics GmbH, Mannheim).

Der Vektor pQE-30 (Qiagen, Hilden, Deutschland) wurde mit den Enzymen BamHI und XmaI vollständig gespalten und das 1,3 kb große DNA Fragment, welches das eno-Gen (Accessionnumber M89931) (Rossol and Pühler, Journal of Bacteriology 174 (9), 2968-2977 (1992)) aus C. glutamicum ATCC 13032 enthält, wurde mit den Enzymen BamHI und XmaI vollständig gespalten. In den Vektor wurde das eno tragende Fragment mit Hilfe der T4-DNA-Ligase ligiert. Die DNA wurde in den E.coli Stamm XL1 Blue (Bullock, Fernandez and Short, BioTechnigues (5) 376-379 (1987)) transformiert. Die Selektion der Transformanten erfolgte auf LB Medium mit Ampicillin 100 mg/l. Das erhaltene Plasmid wird nach Isolierung der DNA mittels Restriktionsspaltung überprüft und im Agarosegel identifiziert. Die DNA-Sequenz des amplifizierten DNA Fragmentes wird durch Sequenzierung überprüft. Die Sequenz des PCR-Produktes stimmt mit der in SEQ ID No. 3 dargestellten Sequenz überein. Das erhaltene Plasmid wird pQE-30eno genannt. The vector pQE-30 (Qiagen, Hilden, Germany) was used completely cleaved the enzymes BamHI and XmaI and that 1.3 kb DNA fragment that contains the eno gene (Accessionnumber M89931) (Rossol and Pühler, Journal of Bacteriology 174 (9), 2968-2977 (1992)) from C. glutamicum ATCC 13032 contains, with the enzymes BamHI and XmaI completely split. In the vector the eno was carried Fragment ligated using the T4 DNA ligase. The DNA was transferred to the E.coli strain XL1 Blue (Bullock, Fernandez and Short, BioTechnigues (5) 376-379 (1987)) transformed. The transformants were selected on LB medium with ampicillin 100 mg / l. The plasmid obtained after isolation of the DNA by restriction cleavage checked and identified in the agarose gel. The DNA sequence of the amplified DNA fragment is by sequencing checked. The sequence of the PCR product agrees with that in SEQ ID No. 3 sequence shown. The received Plasmid is called pQE-30eno.

3.2. Ortsspezifische Mutagenese, Austausch eines Ser gegen ein Glu3.2. Site-specific mutagenesis, exchange of a Ser for a glu

Mit dem QuikChange Site-Directed Mutagenesis Kit (Stratagene, La Jolla, USA) wird die ortsgerichtete Mutagenese durchgeführt. Aufgrund der aus Beispiel 2 bekannten Phosphorylierungsstelle der Enolase für C. glutamicum, das Serin an Position 330, werden folgende Primer-Oligonukleotide für die lineare Amplifizierung ausgewählt:
S330E-1 (SEQ ID No. 13):
5 'CGCTAAGAAGGCTGCCAAC(GAG)ATCCTGGTTAAGGTGAACC 3'
S330E-2 (SEQ ID No. 14):
5' GGTTCACCTTAACCAGGAT(CTC)GTTGGCAGCCTTCTTAGCG 3'. The QuikChange Site-Directed Mutagenesis Kit (Stratagene, La Jolla, USA) is used for site-directed mutagenesis. Based on the phosphorylation site of the enolase for C. glutamicum, the serine at position 330, which is known from Example 2, the following primer oligonucleotides are selected for the linear amplification:
S330E-1 (SEQ ID No. 13):
5 'CGCTAAGAAGGCTGCCAAC (GAG) ATCCTGGTTAAGGTGAACC 3'
S330E-2 (SEQ ID No. 14):
5 'GGTTCACCTTAACCAGGAT (CTC) GTTGGCAGCCTTCTTAGCG 3'.

Die dargestellten Primer werden von der Firma MWG Biotech synthetisiert. Das Codon für Glutamat, welches das Serin an Position 330 ersetzen soll, ist in der oben dargestellten Nukleotidabfolge durch Klammern markiert. Das in Beispiel 3.1. beschriebene Plasmid pQE-30eno wird mit den beiden jeweils zu einem Strang des Plasmides komplementären Primern zur linearen Amplifizierung mittels PfuTurbo DNA Polymerase eingesetzt. Bei dieser Verlängerung der Primer entsteht ein mutiertes Plasmid mit gebrochenen zirkulären Strängen. Das Produkt der linearen Amplifizierung wird mit DpnI behandelt, diese Endonuklease schneidet spezifisch die methylierte und halbmethylierte Template-DNA. Die neusynthetisierte gebrochene, mutierte Vektor-DNA wird in den E.coli Stamm XL1 Blue (Bullock, Fernandez and Short, BioTechniques (5) 376-379 (1987)) transformiert. Nach der Transformation reparieren die XL1 Blue-Zellen die Brüche in den mutierten Plasmiden. Die Selektion der Transformanten erfolgte auf LB Medium mit Ampicillin 100 mg/l. Das erhaltene Plasmid wird nach Isolierung der DNA mittels Restriktionsspaltung überprüft und im Agarosegel identifiziert. Die DNA-Sequenz des mutierten DNA Fragmentes wird durch Sequenzierung überprüft. Die Sequenz des PCR- Produktes stimmt mit der in SEQ ID No. 4 dargestellten Sequenz überein. Das erhaltene Plasmid wird pQE-30enoS330E genannt. The primers shown are from MWG Biotech synthesized. The codon for glutamate, which is the serine Item 330 is to replace is shown in the above Sequence of nucleotides marked by brackets. That in example 3.1. Plasmid pQE-30eno is described with the two each complementary to one strand of the plasmid Primers for linear amplification using PfuTurbo DNA Polymerase used. With this extension the primer a mutated plasmid with broken circular arises Strands. The product of the linear amplification is with DpnI treated, this endonuclease specifically cuts the methylated and semi-methylated template DNA. The newly synthesized broken, mutated vector DNA is used in the E.coli strain XL1 Blue (Bullock, Fernandez and Short, BioTechniques (5) 376-379 (1987)). After The XL1 Blue cells repair the fractures in transformation the mutant plasmids. The selection of the transformants was carried out on LB medium with ampicillin 100 mg / l. The The plasmid obtained is isolated after isolation of the DNA Restriction cleavage checked and in agarose gel identified. The DNA sequence of the mutated DNA fragment is checked by sequencing. The sequence of the PCR Product corresponds to that in SEQ ID No. 4 shown Sequence. The plasmid obtained becomes pQE-30enoS330E called.

3.3. Einbau des eno-Allels in das Chromosom3.3. Incorporation of the eno allele into the chromosome

Das in Beispiel 3.2. erhaltene eno-Allel wird mittels Austauschmutagenese unter Zuhilfenahme des bei Schäfer et al., Gene, 14, 69-73 (1994) beschriebenen sacB-Systems am eno-Genort in das Chromosom von C. glutamicum eingebaut. Dieses System erlaubt dem Fachmann die Identifizierung bzw. die Selektion von Allel-Austauschen, die sich durch homologe Rekombination vollziehen. That in Example 3.2. obtained eno allele is by means of Exchange mutagenesis with the help of the Schäfer et al., Gene, 14, 69-73 (1994) described sacB system on eno locus built into the chromosome of C. glutamicum. This system allows the person skilled in the art to identify or the selection of allele exchanges that are characterized by perform homologous recombination.

3.3.1. Konstruktion des Austauschvektors pK18mobsacBenoS330E3.3.1. Construction of the exchange vector pK18mobsacBenoS330E

Das in Beispiel 3.2. beschriebene Plasmid pQE-30enoS330E wird mit den Restriktionsenzymen EcoRI und HindIII (Gibco Life Technologies GmbH, Karlsruhe, Germany) geschnitten, nach Auftrennung in einem Agarosegel (0,8%) wird mit Hilfe des High Pure PCR Product Purification Kit (Roche, Mannheim, Germany) ein ca. 0,8 kb, die Mutation tragendes, eno-Fragment aus dem Agarosegel isoliert und mit dem bei Schäfer et all, Gene, 14, 69-73 (1994) beschriebenen mobilisierbaren Klonierungsvektor pK18mobsacB zur Ligation eingesetzt. Dieser wird zuvor ebenfalls mit den Restriktionsenzymen EcoRI und HindIII aufgespalten, mit dem ca. 0,8 kb eno-Fragment gemischt und mit T4-DNA-Ligase (Amersham-Pharmaecia, Freiburg, Germany) behandelt. Anschließend wird der E.coli Stamm DH5α (Grant et al.; Proceedings of the National Academy of Sciences USA, 87 (1990) 4645-4649) mit dem Ligationsansatz (Hanahan, In. DNA cloning. A practical approach. Vol 1. ILR-Press, Cold Spring Habor, New York, 1989) transformiert. Die Selektion der Plasmid-tragenden Zellen erfolgt durch Ausplattieren des Tansformationsansatzes auf LB Agar (Sanibrock et al., Molecular Cloning: a laboratory manuel. 2nd Ed. Cold Spring Habor, New York, 1989), der mit 25 mg/l Kanamycin supplementiert wurde. That in Example 3.2. The described plasmid pQE-30enoS330E is cut with the restriction enzymes EcoRI and HindIII (Gibco Life Technologies GmbH, Karlsruhe, Germany), after separation in an agarose gel (0.8%), using the High Pure PCR Product Purification Kit (Roche, Mannheim, Germany) isolated an approximately 0.8 kb mutation-bearing eno fragment from the agarose gel and used for ligation with the mobilizable cloning vector pK18mobsacB described in Schäfer et all, Gene, 14, 69-73 (1994). This is also previously split with the restriction enzymes EcoRI and HindIII, mixed with the approx. 0.8 kb eno fragment and treated with T4 DNA ligase (Amersham-Pharmaecia, Freiburg, Germany). The E.coli strain DH5α (Grant et al .; Proceedings of the National Academy of Sciences USA, 87 (1990) 4645-4649) is then used with the ligation approach (Hanahan, In. DNA cloning. A practical approach. Vol 1. ILR -Press, Cold Spring Habor, New York, 1989). The plasmid-carrying cells are selected by plating the transformation mixture onto LB agar (Sanibrock et al., Molecular Cloning: a laboratory manual. 2 nd Ed. Cold Spring Habor, New York, 1989), which is supplemented with 25 mg / l kanamycin has been.

Plasmid-DNA wird aus einer Transformante mit Hilfe des High Pure Plasmid Isolation Kit der Firma Roche isoliert und durch Restriktionsspaltung mit den Enzymen HindIII/EcoRI und anschließender Agarosegel-Elektrophorese überprüft. Das Plasmid wird pK18mobsacBenoS330E genannt. Eine Karte des Plasmids ist in Fig. 1 dargestellt. Plasmid DNA is isolated from a transformant with the aid of the High Pure Plasmid Isolation Kit from Roche and checked by restriction cleavage with the enzymes HindIII / EcoRI and subsequent agarose gel electrophoresis. The plasmid is called pK18mobsacBenoS330E. A map of the plasmid is shown in Fig. 1.

3.3.2. Austausch des eno-Allels in dem C. glutamicum Stamm DSM57153.3.2. Exchange of the eno allele in the C. glutamicum strain DSM5715

Der in Beispiel 3.3.1 genannte Vektor pK18mobsacBenoS330E wurde mittels Elektroporation (Haynes 1989, FEMS Microbiology Letters 61: 329-334) in den C. glutamicum Stamm DSM5715 transferiert. Der Vektor kann in DSM5715 nicht selbständig replizieren und bleibt nur dann in der Zelle erhalten, wenn er ins Chromosom integriert hat. Die Selektion von Klonen mit integriertem pK18mobsacBenoS330E erfolgt durch Ausplattieren des Elektroporationsansatzes auf LB-Agar (Sambrock et al., Molecular Cloning: a laboratory manuel. 2nd Ed. Cold Spring Habor, New York, 1989), der mit 15 mg/l Kanamycin supplementiert wurde. Angewachsene Klone werden auf LB-Agarplatten mit 25 mg/l Kanamycin ausgestrichen und für 16 Stunden bei 33°C inkubiert. Zur Selektion von Mutanten, bei denen als Folge eines zweiten Rekombinationsereignisses die Exzision des Plasmides mit der Wildtyp-Kopie stattgefunden hat(nach Schäfer et al. 1990, Journal of Microbiology 172: 1663-1666), werden die Klone nach 16stündiger Inkubation in LB- Flüssigmedium auf LB-Agar mit 10% Sucrose angezogen. Das Plasmid pK18mobsacB enthält neben dem Kanamycin- Resistenzgen eine Kopie des für die Levan-Sucrase aus Bacillus subtilis kodierenden sacB-Gens. Die durch Sucrose induzierbare Expression führt zur Bildung der Levan- Sucrase, die die Synthese des für C. glutamicum toxischen Produktes Levan katalysiert. Auf LB-Agar mit Sucrose wachsen daher nur solche Klone an, bei denen das integrierte pK18mobsacBenoS330E wiederum exzisiert hat. In Abhängigkeit von der Lage des zweiten Rekombinationsereignisses in bezug auf den Mutationsort findet bei der Exzision der Allelaustausch bzw. der Einbau der Mutation statt oder es verbleibt die ursprüngliche Kopie im Chromosom des Wirtes. Ungefähr 40 Kolonien werden auf den Phänotyp "Wachstum in Gegenwart von Sucrose" und "Nicht-Wachstum in Gegenwart von Kanamycin" geprüft. 2 Kolonien, die den Phänotyp "Wachstum in Gegenwart von Sucrose" und "Nicht-Wachstum in Gegenwart von Kanamycin" aufweisen, werden mit Hilfe des LightCycler's der Firma Roche Diagnostics (Mannheim, Deutschland) untersucht, um nachzuweisen, daß die Mutation des eno_S330E-Allels im Chromosom vorliegt. Der LightCycler ist ein Kombinationsgerät aus Thermocycler und Fluorimeter. The vector pK18mobsacBenoS330E mentioned in Example 3.3.1 was transferred into the C. glutamicum strain DSM5715 by means of electroporation (Haynes 1989, FEMS Microbiology Letters 61: 329-334). The vector cannot replicate independently in DSM5715 and only remains in the cell if it has integrated into the chromosome. The selection of clones with integrated pK18mobsacBenoS330E is carried out by plating the electroporation batch onto LB agar (Sambrock et al., Molecular Cloning: a laboratory manuel. 2nd and Ed. Cold Spring Habor, New York, 1989), which contains 15 mg / l kanamycin was supplemented. Grown clones are spread on LB agar plates with 25 mg / l kanamycin and incubated for 16 hours at 33 ° C. For the selection of mutants in which the plasmid was excised with the wild-type copy as a result of a second recombination event (according to Schäfer et al. 1990, Journal of Microbiology 172: 1663-1666), the clones are incubated in LB- for 16 hours. Liquid medium grown on LB agar with 10% sucrose. In addition to the kanamycin resistance gene, the plasmid pK18mobsacB contains a copy of the sacB gene coding for the Levan sucrase from Bacillus subtilis. The expression which can be induced by sucrose leads to the formation of levan sucrase, which catalyzes the synthesis of the product levan which is toxic for C. glutamicum. Therefore, only clones in which the integrated pK18mobsacBenoS330E has excised grow on LB agar with sucrose. Depending on the position of the second recombination event in relation to the mutation site, the allele exchange or the incorporation of the mutation takes place during the excision or the original copy remains in the chromosome of the host. Approximately 40 colonies are screened for the "growth in the presence of sucrose" and "non-growth in the presence of kanamycin" phenotype. 2 colonies that have the phenotype "growth in the presence of sucrose" and "non-growth in the presence of kanamycin" are examined using the LightCycler's from Roche Diagnostics (Mannheim, Germany) to demonstrate that the mutation of the eno_S330E- Allele is present in the chromosome. The LightCycler is a combination device consisting of a thermal cycler and a fluorimeter.

In der ersten Phase wird ein ca. 0,3 kb langer, den Mutationsort enthaltender DNA-Abschnitt mittels PCR (Innis et al., PCR protocols. A guide to methods and applications, 1990, Academic Press) unter Verwendung der folgenden Primer-Oligonukleotide amplifiziert.
LC-eno1 (SEQ ID No. 9):
5' TGCAGGAAGATGACTGGGAG 3'
LC-eno2 (SEQ ID No. 10):
5' ACCAGTCTTGATCTGGCCAC 3'. In the first phase, an approximately 0.3 kb long DNA section containing the mutation site is generated by means of PCR (Innis et al., PCR protocols. A guide to methods and applications, 1990, Academic Press) using the following primer oligonucleotides amplified.
LC-eno1 (SEQ ID No. 9):
5 'TGCAGGAAGATGACTGGGAG 3'
LC-eno2 (SEQ ID No. 10):
5 'ACCAGTCTTGATCTGGCCAC 3'.

In der zweiten Phase wird mit zwei zusätzlichen, unterschiedlich langen und mit verschiedenen Fluoreszenzfarbstoffen (LightCycler(LC)-Red640 und Flourescein) markierten Oligonukleotiden, die im Bereich des Mutationsortes hybridisieren, mit Hilfe der "Fluorescence Resonance Energy Transfer"-Methode (FRET) das Vorhandensein der Mutation mit einer Schmelzkurvenanalyse (Lay et al., Clinical Chemistry, 43: 2262-2267 (1997)) nachgewiesen.
eno330EC (SEQ ID No. 11):
5' LC-Red640 - AACCAGGATCTCGTTGGCAGC - (P) 3'
eno330-A (SEQ ID No. 12):
5' CGAAGGTCTCGGTGAGGGTACCGATCTGGTTCACC - Fluorescein 3'. In the second phase, two additional oligonucleotides, of different lengths and labeled with different fluorescent dyes (LightCycler (LC) -Red640 and Flourescein), which hybridize in the area of the mutation site, are used with the "Fluorescence Resonance Energy Transfer" (FRET) method Presence of the mutation with a melting curve analysis (Lay et al., Clinical Chemistry, 43: 2262-2267 (1997)) detected.
eno330EC (SEQ ID No. 11):
5 'LC-Red640 - AACCAGGATCTCGTTGGCAGC - (P) 3'
eno330-A (SEQ ID No. 12):
5 'CGAAGGTCTCGGTGAGGGTACCGATCTGGTTCACC - Fluorescein 3'.

Die dargestellten Primer für die PCR werden von der Firma MWG Biotech und die dargestellten Oligonukleotide für die Hybridisierung von der Firma TIB MOLBIOL (Berlin, Deutschland) synthetisiert. The primers shown for the PCR are from the company MWG Biotech and the oligonucleotides shown for the Hybridization from the company TIB MOLBIOL (Berlin, Germany) synthesized.

Auf diese Weise wurde ein Klon identifiziert, der an der Position 988-990 der Kodierregion des eno-Gens das Basen- Triplett Guanin-Adenin-Guanin enthält und somit das eno_S330E-Allel besitzt (SEQ ID No. 5). Dieser Klon wurde als Stamm C. glutamicum DSM5715enoS330E bezeichnet. In this way, a clone was identified that was on the Position 988-990 of the coding region of the eno gene the base Contains triplet guanine-adenine-guanine and thus that eno_S330E allele has (SEQ ID No. 5). This clone was referred to as strain C. glutamicum DSM5715enoS330E.

Beispiel 4Example 4 Auswirkung der in Beispiel 3 durchgeführten Mutagenese auf die Lysinproduktion in einem ModellstammEffect of the mutagenesis carried out in Example 3 on the lysine production in a model strain Herstellung von L-LysinProduction of L-lysine

Der in Beispiel 3 erhaltene C. glutamicum Stamm DSM5715SenoS330E wurde in einem zur Produktion von L-Lysin geeigneten Nährmedium kultiviert und der L-Lysingehalt im Kulturüberstand bestimmt. The C. glutamicum strain obtained in Example 3 DSM5715SenoS330E was used in a production of L-lysine cultivated suitable nutrient medium and the L-lysine content in Culture supernatant determined.

Dazu wurde der Stamm zunächst auf Agarplatte (Hirn-Herz Agar) für 24 Stunden bei 33°C inkubiert. Ausgehend von dieser Agarplattenkultur wurde eine Vorkultur angeimpft (10 ml Medium im 100 ml Erlenmeyerkolben). Als Medium für die Vorkultur wurde das Vollmedium CgIII verwendet. Medium Cg III NaCl 2,5 g/l Bacto-Pepton 10 g/l Bacto-Yeast-Extrakt 10 g/l Glucose (getrennt autoklaviert) 2% (w/v) Der pH-Wert wurde auf pH 7.4 eingestellt For this purpose, the strain was first incubated on agar plate (brain-heart agar) for 24 hours at 33 ° C. Starting from this agar plate culture, a pre-culture was inoculated (10 ml medium in 100 ml Erlenmeyer flask). The complete medium CgIII was used as the medium for the preculture. Medium Cg III NaCl 2.5 g / l Bacto-peptone 10 g / l Bacto-yeast extract 10 g / l Glucose (autoclaved separately) 2% (w / v) The pH was adjusted to pH 7.4

Die Vorkultur wurde 24 Stunden bei 33°C bei 250 rpm auf dem Schüttler inkubiert. Von dieser Vorkultur wurde eine Hauptkultur angeimpft, so daß die Anfangs-OD (660 nm) der Hauptkultur 0,1 OD betrug. Für die Hauptkultur wurde das Medium MM verwendet. Medium MM CSL (Corn Steep Liquor) 5 g/l MOPS 20 g/l Glucose (getrennt autoklaviert) 50 g/l Salze (NH4)2SO4) 25 g/l KH2PO4 0,1 g/l MgSO4.7 H2O 1,0 g/l CaCl2.2 H2O 10 mg/l FeSO4.7 H2O 10 mg/l MnSO4.H2O 5,0 mg/l Biotin (sterilfiltriert) 0,3 mg/l Thiamin.HCl (sterilfiltriert) 0,2 mg/l Leucin (sterilfiltriert) 0,1 g/l CaCO3 25 g/l The preculture was incubated on the shaker at 33 ° C. at 250 rpm for 24 hours. A main culture was inoculated from this preculture so that the initial OD (660 nm) of the main culture was 0.1 OD. The medium MM was used for the main culture. Medium MM CSL (Corn Steep Liquor) 5 g / l PUG 20 g / l Glucose (autoclaved separately) 50 g / l (NH 4 ) 2 SO 4 ) 25 g / l KH 2 PO 4 0.1 g / l MgSO 4 .7 H 2 O 1.0 g / l CaCl 2 .2 H 2 O 10 mg / l FeSO 4 .7 H 2 O 10 mg / l MnSO 4 .H 2 O 5.0 mg / l Biotin (sterile filtered) 0.3 mg / l Thiamin.HCl (sterile filtered) 0.2 mg / l Leucine (sterile filtered) 0.1 g / l CaCO 3 25 g / l

CSL, MOPS und die Salzlösung werden mit Ammoniakwasser auf pH 7 eingestellt und autoklaviert. Anschließend werden die sterilen Substrat- und Vitaminlösungen sowie das trocken autoklavierte CaCO3 zugesetzt. CSL, MOPS and the salt solution are adjusted to pH 7 with ammonia water and autoclaved. The sterile substrate and vitamin solutions and the dry autoclaved CaCO 3 are then added.

Die Kultivierung erfolgt in 10 ml Volumen in einem 100 ml Erlenmeyerkolben mit Schikanen. Die Kultivierung erfolgte bei 33°C und 80% Luftfeuchtigkeit. The cultivation takes place in a volume of 10 ml in a 100 ml Erlenmeyer flasks with baffles. The cultivation took place at 33 ° C and 80% humidity.

Nach 72 Stunden wurde die OD bei einer Meßwellenlänge von 660 nm mit dem Biomek 1000 (Beckmann Instruments GmbH, München) ermittelt. Die gebildete L-Lysinmenge wurde mit einem Aminosäureanalysator der Firma Eppendorf-BioTronik (Hamburg, Deutschland) durch Ionenaustausch-Chromatographie und Nachsäulenderivatisierung mit Ninhydrindetektion bestimmt. After 72 hours the OD was measured at a measuring wavelength of 660 nm with the Biomek 1000 (Beckmann Instruments GmbH, Munich) determined. The amount of L-lysine formed was included an amino acid analyzer from Eppendorf-BioTronik (Hamburg, Germany) by ion exchange chromatography and post-column derivatization with ninhydrin detection certainly.

In Tabelle 1 ist das Ergebnis des Versuchs dargestellt. Tabelle 1

Table 1 shows the result of the experiment. Table 1

Folgende Figuren sind beigefügt:
Fig. 1: Karte des Plasmids pK18mobsacBenoS330E. The following figures are attached:
Fig. 1: Map of the plasmid pK18mobsacBenoS330E.

Die verwendeten Abkürzungen und Bezeichnungen haben folgende Bedeutung.
KanR: Kanamycin Resistenz-Gen
HindIII: Schnittstelle des Restriktionsenzyms HindIII
EcoRI: Schnittstelle des Restriktionsenzyms EcoRI
enoS330E: mutiertes eno-Allel
sacB: sacB-Gen
RP4mob: mob-Region mit dem Replikationsursprung für den Transfer (oriT)
oriV: Replikationsursprung V. SEQUENZPROTOKOLL





















The abbreviations and designations used have the following meaning.
KanR: Kanamycin resistance gene
HindIII: Interface of the restriction enzyme HindIII
EcoRI: Interface of the restriction enzyme EcoRI
enoS330E: mutated eno allele
sacB: sacB gene
RP4mob: mob region with the origin of replication for the transfer (oriT)
oriV: origin of replication V. SEQUENCE LIST





















Claims (12)

1. Mikroorganismus, in dem wenigstens ein Protein in seiner Phosphorylierbarkeit dauerhaft so geändert ist, daß eine gegenüber dem Wildtyp erhöhte Biosynthese wenigstens einer von dem Mikroorganismus synthetisierten Feinchemikalie erreicht wird. 1. microorganism in which at least one protein in its Phosphorylability is permanently changed so that a at least increased biosynthesis compared to the wild type one synthesized by the microorganism Fine chemical is achieved. 2. Mikroorganismus nach Anspruch 1, dadurch gekennzeichnet, daß der Mikroorganismus gentechnisch verändert ist. 2. Microorganism according to claim 1, characterized in that that the microorganism is genetically modified. 3. Mikroorganismus nach Anspruch 1, dadurch gekennzeichnet, daß der Mikroorganismus ein Prokaryot, ein Eukaryot oder ein Archea ist. 3. Microorganism according to claim 1, characterized in that the microorganism is a prokaryote, a eukaryote or is an archea. 4. Mikroorganismus nach Anspruch 3, dadurch gekennzeichnet, daß er Bakterium ist ausgewählt aus der Gruppe der coryneformen Bakterien und Enterobacteriaceaeoder ein Pilz aus der Gruppe der Hefen. 4. Microorganism according to claim 3, characterized in that that he is selected from the group of bacteria coryneform bacteria and Enterobacteriaceae or Mushroom from the yeast group. 5. Mikroorganismus nach einem der Ansprüche 1 bis 4, dadurch gekennzeichnet, daß das Protein ein Enzyme, ein Protein des Biosynthesewegs der entsprechenden Feinchemikalie, deren Synthese gewünscht ist, insbesondere Schlüsselenzyme des Synthesewegs ist. Ein regulatorisches Protein, eine Komponente eines Signaltransduktionswegs, ein zellintegrität vermittelnes Protein, ein Speicherprotein, ein Transportprotein oder ein Immunprotein ist. 5. Microorganism according to one of claims 1 to 4, characterized in that the protein is an enzyme, a Protein of the corresponding biosynthetic pathway Fine chemical whose synthesis is desired especially key enzymes of the synthetic pathway. On regulatory protein, a component of a Signal transduction pathways, a cell integrity mediator Protein, a storage protein, a transport protein or is an immune protein. 6. Mikroorganismus nach einem der Ansprüche 1 bis 5, dadurch gekennzeichnet, daß die hergestellte Feinchemikalie eine Aminosäure, ein Vitamin, ein Nukleosid oder ein Nukleotid ist. 6. Microorganism according to one of claims 1 to 5, characterized in that the manufactured Fine chemical an amino acid, a vitamin, a Is a nucleoside or a nucleotide. 7. Mikroorganismus nach einem der Ansprüche 1 bis 6, dadurch gekennzeichnet, daß in dem Protein die Änderung der Phosphorylierbarkeit aufgrund einer Mutation in wenigstens einer Aminosäure des Proteins gegenüber der Wildtypsequenz besteht. 7. Microorganism according to one of claims 1 to 6, characterized in that the change in the protein the phosphorylability due to a mutation in at least one amino acid of the protein over the Wild type sequence exists. 8. Mikroorganismus nach Anspruch 7, dadurch gekennzeichnet, daß die Mutation ein Aminosäureaustausch, eine Deletion oder eine Insertion wenigstens einer Aminosäure ist. 8. Microorganism according to claim 7, characterized in that the mutation is an amino acid exchange, a deletion or is an insertion of at least one amino acid. 9. Mikroorganismus nach Anspruch 8, dadurch gekennzeichnet, daß ein Aminosäureaustausch einer im Wildtyp reversibel phosphorylierbaren Aminosäure, oder ein Aminosäureaustausch im Bereich der Phosphorylierungssequenz eines Proteins vorliegt. 9. Microorganism according to claim 8, characterized in that an amino acid exchange is reversible in the wild type phosphorylatable amino acid, or a Amino acid exchange in the area of Phosphorylation sequence of a protein is present. 10. Mikroorganismus nach Anspruch 9, dadurch gekennzeichnet, daß ein Austausch eines im Wildtyp phosphorylierbaren Serins, Threonins, Tyrosins, Histidins, Glutamats, Aspartats, Arginins oder Lysins gegen eine andere Aminosäure vorliegt. 10. Microorganism according to claim 9, characterized in that an exchange of a phosphorylatable in the wild type Serins, Threonins, Tyrosins, Histidins, Glutamats, Aspartate, arginine or lysine against another Amino acid is present. 11. Mikroorganismus nach Anspruch 10, dadurch gekennzeichnet, daß ein Austausch einer im Wildtyp nicht phosphorylierten Aminosäure gegen ein Serin, Threonin, Tyrosin, Aspartat, Glutamat Histidin, Arginin oder Lysin vorliegt. 11. Microorganism according to claim 10, characterized characterized that an exchange of a wild type is not phosphorylated amino acid against a serine, threonine, Tyrosine, aspartate, glutamate, histidine, arginine or lysine is present. 12. Verfahren zur Herstellung von Feinchemikalien oder Metaboliten unter Verwendung eines Mikroorganismus nach einem der Ansprüche 1 bis 11. 12. Process for the production of fine chemicals or Metabolites using a microorganism one of claims 1 to 11.
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