DE10050424A1 - Preparation of a jade extract by pre-processing nephrite jade ore, mixing with water and distilling, useful as a health drink containing additives and pigments for promoting metabolism of the human body and treating pathological symptoms - Google Patents

Abstract

Jade extract obtained from a nephrite-jade distilled solution to economically form a distilled product for use as general practical goods A jade extract is obtained from a nephrite-jade distilled solution such that nephrite jade ore is pre-processed by pulverizing, sorting and cleaning, and mixing with distilled water at an evaporator in ratio 1:4. The mixture obtained is heated at 100 deg C or more, such that components eluted from the ores into the water during boiling are vaporized with the water and the vapor obtained converted into a distilled solution through heat exchange. An Independent claim is also included for the stepwise preparation of the jade extract.

Description

BACKGROUND OF THE INVENTION 1. Field of the invention

The present invention relates to a jade extractant and method for the preparation thereof, and in particular on a jade extractant for obtaining an egg A nephrite jade distilled solution by mixing pure, distilled water and Nephrite jade of a very fine combination binding fiber microstructure of a cryptocrite stallinen tremolite type, obtained from a nephrite jade mine (Chuncheon, Ko rea), which is distilled, and converting the temperature of nephrite jade steam, which is generated.

2. Description of the related art

It is common knowledge that jade is largely divided into jadeite and nephrite jade and Jadeit belongs to the Pyroxen family and has a monoclinic system, the Has silica, aluminum oxide and soda or soda. It is an intimate crowd, and the hardness is comparable to that of crystal. It is transparent or translucent of a black, teal or green color. One usually calls the yes deit as "jade". Nephrite jade is an inorganic material that is a monoclinic system from ionic silicates. The quality of nephrite jade is due to the fine structure true, and the finer the fiber, the better the quality (RESOURCES PROMO TION, 1993, Korea Resources Corporation).  

According to a German literature by Mauda Palmer "The hidden power of crystals and of gemstones "have two different ores, jadeite and nephrite jade, both silicon and oxygen, which, like most other gemstones, contains a lot of crystals and have aggregates of microparticles comprising the elements Ca, Fe and Mg, the are good for the human body, while jadeite baking soda and an aluminum compo nente. Accordingly, it has been reported recently that nephrite jade when it is applied to the body, an essential effect in the treatment of Has hypertension, diabetes, circulatory disorder, heart disease and kidney disease.

A classic work of traditional oriental medicine "Treasures in Oriental Medici ne ", describes that when jade is added to black rice liquor, it is about to change the liqueur to water, and adding jade powder in one size similar to sesame seeds, is good for draining. It also describes that when jade Powder (1 part by volume), rice (1 part by volume) and white dew (1 part by volume) cooked with rice in a copper kettle the jade powder becomes water (so-called jade liqueur, "divine jade water" - divine jade water ")." Plants of Divine Agriculture "," Plants of Tang Age "and" List of Basic Plants "describe that taking jade powder in a size similar to Se the 5 viscera (viscera) and 6 viscera enriched and completely removes the slag materials. In addition, it is effective for the digestive system which removes heat from the stomach and is good for treating bron chial asthma, body fever and heaviness in the chest, as well as against thirst. If Jade powder is taken for a long time, the body becomes light, the function of the Lungs are raised and vocal cords make it easier to make sounds. Also it is good for the throat, the nourishment of the hair, the functions of the 5 viscera and the 6 viscera and the treatment of nerve disorders, such as wise stress.

In addition, the components of nephrite jade show excellent functions in the Body without a side effect. For example, taking white jade powder good for tension and against cramps in muscles, and rubbing with nephrite jade on the sore skin for a few days eliminates the suffering.  

However, nephrite jade is not present in nature in a large amount, causing the use of it on noble, personal ornaments, such as necklaces, Ring, bracelet or the like, in view of the well-known, excellent, medical functions is limited. Additionally requires the processing of nephrite the knowledge of experts who have a lot of experience and nephrite jade is in economi disadvantageous in that it has a very high price, so that Development of general, practical goods using nephrite jade is intensively needed is done.

By combining the excellent medical effects of Ne Phrit-Jade has received attention, the applicant of the present Invention carried out intensively for many years of studies and various patents in Korea and other countries, including Korean Patent No. 112 812 entitled "Glass Products Containing Nephrite Jade Powder and Preparation Method there of ".

SUMMARY OF THE INVENTION

Therefore, it is an object of the present invention to provide a jade extractant for Obtained from a nephrite-jade distilled solution by mixing pure, distilled water and jade ore (a very fine combination binding fiber microstructure of the cryptocrystalline tremolite type) in an evaporator in a mixing ratio of about 1: 4 to heat the mixture to a high temperature and heat Exchange of the steam generated therein for the same into a distilled solution convert from nephrite jade, which is a jade extractant, to get that there due to medical effects for the treatment of pathological symptoms (head pain, numbness, indigestion, insomnia or the like) and Has support for the growth of plants and animals, and a preparation ver drive to get for it.

In order to achieve the above object of the present invention, a jade Extractant created to obtain a nephrite-jade distilled solution, wherein Nephrite jade ore processed by pulverizing, sorting and cleaning tet, and sterile, distilled water under evaporation in a mixture ratio  of 1: 4, the mixture being heated to a high temperature of 100 ° C or higher is heated, taking components from the jade ore into the destil water is eluted or dissolved out while boiling, with the de still water are evaporated, and the steam is transferred to a distilled solution a heat exchange is converted.

According to another aspect of the present invention there is provided a method for preparing created a jade extractant that has the steps of pulverizing Jade ore in a predetermined size, sorting and cleaning it to a pre Complete processing step, prepare distilled water, mix the preprocessed jade ore and distilled water in one evaporator in one Mixing ratio of 1: 4, heating the same at a temperature of 100 ° C or higher to enable the materials containing the jade ore to be included in the de still water are eluted, with which distilled water is evaporated, and the Steam containing the jade ore containing materials used in the evaporation step are generated, is converted in a distilled solution via a heat exchange.

Here is the jade ore cryptocrystalline tremolite with a negative value of σ ¹⁸ O, which has a composition which is given below.

Semi-quantitative analysis of the nephrite jade powder used in the present invention (%) silicon 34,00 magnesium 10.00 calcium 4.90 iron 0.23 aluminum 0.16 copper 0.17 cobalt 0.046 manganese 0.14 tin 0.024 beryllium 0.00072 silver 0.0013 titanium 0.0038 nickel 0.0028 chrome 0.0030 other elements 0.0000

Also with regard to the mixing ratio of jade ore and distilled water The more jade ore is in the mix, the stronger the effects of jade  improved. However, the manufacturing or preparation costs increase due to the expensive jade ore. As a result, jade ore and distilled water are previously combined in one Mixing ratio of 1: 4 mixed.

The distilled water used in the present invention is not specific fish restricted, but distilled water with no contaminants, which is its own of jade ore can be used appropriately the.

BRIEF DESCRIPTION OF THE DRAWINGS

The above object and the advantages of the present invention will be apparent from the Description in detail of a preferred embodiment thereof with reference to FIG the accompanying drawings can be seen, in which:

FIG. 1 is a schematic diagram showing an apparatus for preparing a jade extractant according to the present invention;

Fig. 2 shows a crystalline structure of jade powder used in the present inven tion by a scanning electron microscope (SEM) image;

Fig. 3 is a graph showing exposure rates in far infrared radiation rates of nephirite jade used in the present invention as measured by an FT-IR spectrometer;

FIGS. 4A and 4B show typical chromatograms of a standard solution (A) which is used in the present invention and a sample (B) a cryptocrystalline Tremo lit-microstructure;

Fig. 5 illustrates the structure of a tested cryptocrystalline tremolite microstructure in the striatum used in the present invention (above) and in the core accumbens (below);

Fig. 6 is a graph showing the effect of a nephrite jade extractant on the responsiveness of dopanine release to methamethamine in the striatum used in the present invention, and

Figure 7 is a graph showing the effect of a nephrite jade extractant on the responsiveness of dopamine release to nicotine in the striatum used in the present invention;

Fig. 8 is a graph showing the change in activity of a control group (rats) after drinking jade water used in the present invention;

FIG. 9A and 9B show SEM photographs of silk, which is not treated with jade water, which is used in the present invention;

Figures 9C and 9D show SEM photographs of silk not treated with jade water used in the present invention;

Fig. 10 is a graph showing the change in volume of the entire culture medium for cultivating Digitalis Lanata;

Fig. 11 is a graph showing the change in cell volume of Digita lis Lanata;

Fig. 12 is a graph showing the change in the weight of the biological organisms of the cell;

Fig. 13 is a graph showing the change in dry weight of the cells;

Fig. 14 is a graph showing the change in pH in the culture medium for Digitalis Lanata;

Fig. 15 is a graph showing the change in pH of the culture medium after adding nephrite jade powder;

FIG. 16 illustrates the state of the analytical instrument;

Fig. 17 shows the expression of the results of the analysis;

Fig. 18 illustrates the change in pH of a jade necklace and jade ore in ordinary tap water;

Fig. 19 shows the change in pH of a jade necklace and jade ore in the groundwater with time;

Fig. 20 shows the change in pH of a strong acid solution treated with jade powder used in the present invention, and

Fig. 21 shows the change with time in a strong acid solution in a milk bottle containing jade powder used in the present invention and stored in an ordinary milk bottle with time.

DESCRIPTION OF THE PREFERRED EMBODIMENTS

Preferred embodiments of a jade extractant according to the present Invention and a preparation method thereof will now be described with reference to the attached drawings.

Fig. 1 shows a schematic diagram of an evaporation apparatus 100 for centering a Präpa Jade extraction means according to the present invention, and an example of a dissection of the Jade extractant is by reference beschrie be ben.

First, nephrite jade ore, available from Chuncheon, Korea, defined as cryptocrystalline tremolite having a negative value of σ ¹⁸ O, is pulverized using an ordinary pulverizer, sorted by a sorting process, and then cleaned to complete the preprocessing step.

The so-processed jade ore 102 and the pure, distilled water 104 , which was prepared in advance, are introduced into an evaporator 106 in a ratio of 1: 4. Then, a flame nozzle 108 disposed in the lower portion of the evaporator 106 is operated to uniformly heat the evaporator 106 to a high temperature of 100 ° C. or higher by heat generated by the flame nozzle 108 . Accordingly, the contents in the evaporator 106 are heavily cooked. In cooking, components that are eluted from the jade ore 102 into the distilled water 104 , such as silicon, magnesium, calcium, etc., are evaporated with the distilled water 104 to then flow through a drain line 110 . When the steam passes through the discharge line 110 , the steam is liquefied due to a temperature difference between the discharge passage 110 and the inside of the evaporator, and flows along a passage, and then drips into a collection container. Here, the steam that passes directly through the drain passage 110 is subjected to heat exchange at the time when it passes through a heat exchanger 120 installed near a drain end of the drain passage 110 to then be completely liquefied. It is necessary to refill the distilled water accordingly during the evaporation time. Also, since the heat exchanger 120 has a general configuration, a detailed explanation thereof will not be made. The jade extractant obtained as described above can be used to support the metabolism of the human body, to treat diseases, for example as a Ringer's solution such as glucose. The jade extractant can also be used for drinking as a health (sports) ion drink (e.g. Gator-Ade or Pacari-Sweat), as a dietary fiber drink (e.g. Mieor-Fiber), food tonic (e.g. Bachhus or Wonbi-D) and other carbonated beverages, by adding them to a small amount of additives and pigments or other dyes. The experimental results are given below.

Experimental example 1

The experimental examples shown in Tables 1 to 4 below, which to enhance the effect of nephrite jade used in the present invention and to study the effect of nephrite jade on a living body.  

Table 1

Lead content test

Table 2

Heavy metals test (including Pb)

Table 3

Test for dissociation of inorganic materials

The sample (100 g) was extracted with 1 liter of water in an autoclave, and the extract was analysed.

Table 4

As shown from the above experimental result, since the Ne phrit-jade used in the present invention does not ent any material that has toxicity to the human body, such as lead, Heavy metals or other materials confirm that it is safe if it is for plants or animals is used.

Experimental example 2

The IR study of nephrite jade powder was carried out in this experiment ( Fig. 2).

Table 5

IR condition

Phase: thin film liquefied with tetrahydrofuran.
Results: The IR spectrum appears to match polycarbonate resin patterns and emit the electromagnetic wave, which has a wavelength of 6 - 52 µ.

Experimental example 3

The COD (Chemical Oxygen Demand) and BOD (Biochemical Oxygen Demand) from Ja de powder used in this experimental example was tested so as shown in Tables 6 and 7 below.  

Table 6

Table 7

Table 8

Dermal irritation study

Procedure: Federal Register, Vol. 43, No. 163rd
CTFA (The Cosmetic, Toiletry and Fragrance Association, Washington, DC).

Technical guideline procedure

The procedure that a patch test or irritation test Technique used on the scraped and intact skin of the albino rabbit. The hair was cut from the back and the flanks or thighs. Two areas  of the back, about 10 cm apart, were used for the position of the plum stipulated. One area was scraped (abraded) by 4 small, epidermal Incisions or incisions (2 perpendicular to each other in a "tic-tac-toe" pattern) in the area of the pavement. The 1 inch square patches a surgical gauze was made using thin rubber bands and an adhesive bebands attached at the relevant point. The sample was placed under the 0.5 ml patch (g) brought at every point. The entire body (trunk) of the animal was then covered with wrapped in a rubber-coated bandage for a period of 24 hours. The animals were immobilized during the exposure period. After removing the patch was the reaction at each point is evaluated based on the weighted cuts, which follows is described.

Evaluation of skin reactions Erythema and scab formation

No erythema - 0
Very light erythema (just barely noticeable) - 1
Well-defined erythema - 2
Moderate to serious erythema - 3
Serious erythema (beet blush) to slight scab formation (deep wounds) - 4

Edema formation

No edema - 0
Very slight edema (just barely noticeable) - 1
Slight edema (edges of the area are well defined by elevation) - 2
Moderate edema (approximately 1 mm raised) - 3

Table 9

Conclusion: The sample is set to be and is free from skin irritation therefore suitable for cosmetic use in skin care products.

Table 10

Acute oral toxicity (0.5 g / 100 g weight) test

Table 11

Preparation of a sample: The sample was extracted by boiling with sterile, distilled water for 10 minutes. The extract was administered through a cannula at 0.5 g per 100 g of animal weight.
Conclusion: The sample confirms the standard statement that it should have no oral toxicity.

The following experiment (Experimental Example 4) was carried out by the Department of Nuclear Medicine, Samsung Medical Center, affiliated with Sungkyunkwan University School of Medicine, Seoul, Korea, performed the effect of nephrite jade water to examine, which is obtained by the above embodiments, the meaning a jade extractant, based on the results of brain activity of the experimental examples described above are confirmed. The expe The experimental procedures and the results are as follows.  

Experimental example 4 A. Effect of jade extractant according to the present invention on a dopa min release depending on methamphetamine 1. Experimental animals

Internal microdialysis experiments were performed using male sprague Dawley mellitus rats (280-320 g, Korea Experimental Animal Center). The Animals were placed in 12: 12 day / night chambers, with one free Access to food and water.

2. Internal microdialysis experiment a) Treatment with medicine

To see the effect of a jade extractant on dopamine release To examine response to methamphetamine, 0.5 ml of jade Extraction agent according to the present invention intraperitoneally 60 minutes before an intraperitoneal injection of methamphetamine (10 mg / kg, i.p.), and then dialysate samples were collected from the striatum.

b) Administration and microdialysis

The experimental animals were anesthetized with pentobarbital (50 mg / kg, ip) and then a guide cannula was inserted into the striatum (Sterotaxic coordinates: AP, 1.0 and L, 3.2, in relation to the bregma, H, 3, 0 in relation to the dura-mater) used in a non-invasive manner by the Atlas of Paxinos & Watson (1986) and fixed using a micro thread and dental cement. After a recovery period of 24 hours, a 4 mm vertical microdialysis probe (CMA-12, Carnegie-Medicine, Stockholm, Sweden) was inserted over the guide cannula. The inlet of the microdialysis probe was connected to a syringe which was mounted on a feed pump via a dual liquid rotating ring. While supplying an artificial cerebrospinal fluid containing 145 mM NaCl, 2.7 mM KCl, 1.2 mM CaCl ₂ , 2.0 mM Na ₂ HPO ₄ , pH 7.4, under a flow rate of 1.5 μ l / min. via the microdialysis probe, dialysates were collected at 20 minute intervals using a microfraction collector supplied by Carnegie-Medicine connected to the outlet of the microdialysis probe. The microdialysis experiment was carried out on experimental animals that were allowed to move freely.

c) Analysis of microdialysate samples

Monoamines and metabolites from microdialysate samples were electroplated by an HLPC chemical detection (ECD) system analyzed. The dialysate (30 µl dose) was taken under Use of a reversed phase column (Waters Nova-Pak C-18 (4 µm, 150 times 3.9 mm) separated. The mobile phase consisted of 75 mM sodium phosphate, 0.12 mM EDTA, 1.4 mM Octanesulfonic acid and 10% acetonitrile. It was adjusted to pH with phosphoric acid Value of 3.2 set. The HPCL peak was determined using an ESA Coulochem II 5200A electrochemical detector with a high performance analyzer (ESA, model 5014) analyzed. The potential of a working electrode was set to + 320 mV. The System flow rate was 1.0 ml / min.

Typical chromatograms obtained from the standard solution and microdialysate samples are shown in FIG. 4. Dopamine, dihydroxyphenylacetate acid (DOPAC), ho movanillic acid (HVA), 5-hydroxytrymptamine (5-HT), 5-hydroxyindoleacetate acid (5-HIAA) were differentiated separately. The dopamine concentration of the dialysate was more than 3 times the minimum measurement limit, ie 116 pM. The baseline concentrations of dopamine, DOPAC, HVA, 5-HT and 5-HIAA were 18.5 nM, 90.5 nM, 74.7 nM, 6.2 nM and 45.6 nM, respectively. Since the dopamine recovery rate of the microdialysis probe obtained by external experimentation was 20%, the extracellular dopamine level was estimated to be 92.5 nM. The dopamine level of the microdialysis probe was expressed as a percentage of base in the average dopamine concentration of the last 3 samples collected immediately before the experimental treatment.

d) Histological identification

At the end of the experiments, the experimental animals were anesthetized with an overdose of pentobarbital and then decerebrated by infusion of a physiological saline solution and a 10% formalin solution. The removed brains were kept in a 10% formalin solution for at least 2 weeks. Then tissue from a section where the microdialysis probe was attached was cut to a thickness of 50 µm, and its exact positions were identified by histological hematoxylin staining (see Fig. 5).

e) Statistical analysis

All measured values were expressed as an average ± S.E. The statistical Si The significance of the data was assessed using a multiple analysis of the variance (med time × time) and analyzed using the Bonferroni method. StarView 4.02 (Abacus Concepts, Inc., Berkeley, Ca., USA) as statistical analysis software and Macintosh Computers were used. Statistical significance was based on the P- Level of 0.005 determined.

3. Test results

Fig. 6 illustrates the effect of a jade extractant (distilled solution) with a dopamine release response to methamphetamine in the striatum. After an injection of methamphetamine (10 mg / kg, ip), the extracellular concentration of dopamine increased gradually to peak in 60 minutes (433.6 ± 14.3% baseline). When 0.5 ml of jade extractant was injected intraperitoneally 60 minutes before a methamphetamine infusion, an increase in the extracellular concentration of dopamine in the striatum increased up to 32.8% in response to methamphetamine (29.15 ± 11 , 4% of baseline). Injection of jade extractant also inhibited the time required to reach the maximum level of dopamine after methamphetamine infusion. The jade extractant according to the present invention did not affect the extracellular concentration of dopamine in the stable striatum.

B. Effect of jade extractant according to the present invention on a dopamine Release response to nicotine 1. Experimental animals

Internal microdialysis experiments were performed using male sprague Dawley mellitus rats (280-320 g, Korea Experimental Animal Center). The Animals were placed in 12: 12 light / dark states, with one free Access to food and water.

2. Internal microdialysis experiment a) Treatment with medicine

The effect of a jade extractant on a dopamine release response to examine nicotine, 0.5 ml of jade extractant according to the available intraperitoneally 60 minutes before an infusion of nicotine (0.4 mg / kg, hyperdermal injection), and then dialysate samples were taken from the striatum collected.

b) Administration and microdialysis

The experimental animals were anesthetized with pentobarbital (50 mg / kg, ip) and then a guide cannula was inserted into the striatum (Sterotaxic coordinates: AP, 1.0 and L, 3.2, in relation to the bregma, H, 3, 0 in relation to the dura-mater) used in a non-invasive manner by the Atlas of Paxinos & Watson (1986) and fixed using a micro thread and dental cement. After a recovery period of 24 hours, a 4 mm vertical microdialysis probe (CMA-12, Carnegie-Medicine, Stockholm, Sweden) was inserted over the guide cannula. The inlet of the microdialysis probe was connected to a syringe which was mounted on a feed pump via a dual liquid rotating ring. While supplying an artificial cerebrospinal fluid containing 145 mM NaCl, 2.7 mM KCl, 1.2 mM CaCl ₂ , 2.0 mM Na ₂ HPO ₄ , pH 7.4, under a flow rate of 1.5 μ l / min. via the microdialysis probe, dialysates were collected at 20 minute intervals using a microfraction collector supplied by Carnegie-Medicine connected to the outlet of the microdialysis probe. The microdialysis experiment was carried out on experimental animals that were allowed to move freely.

c) Analysis of microdialysate samples

The concentrations of microdialysate samples were electrochemical by HLPC Acquisition (ECD) system analyzed. The dialysate (30 µl dose) was used a reverse phase column (Waters Nova-Pak C-18 (4 µm, 150 times 3.9 mm) separated mobile phase consisted of 75 mM sodium phosphate, 0.12 mM EDTA, 1.4 mM octarisul fonic acid and 10% acetonitrile. It was adjusted to pH 3.2 with phosphoric acid set. The HPCL peak was determined using an ESA Coulochem II 5200A Electrochemical detector with a high performance analyzer (ESA Model 5014) analyzed. The potential of a working electrode was set to +320 mV. The currents system flow rate was 1.0 ml / min. The dopamine concentration of the dialysate was calculated as a percentage of baseline in the average dopamine concentrations last 3 samples expressed immediately prior to experimental treatment were collected.

d) Histological identification

At the end of the experiments, the experimental animals were anesthetized with an overdose of pentobarbital and then decerebrated by infusion of a physiological saline solution and a 10% formalin solution. The removed brains were kept in a 10% formalin solution for at least 2 weeks. Then tissue from a section where the microdialysis probe was stored was cut to a thickness of 50 µm, and its exact positions were identified by hematoxylin staining (see Fig. 5).

e) Statistical analysis

All measured values were expressed as an average ± S.E. The statistical Si The significance of the data was assessed using a multiple analysis of the variance (med time × time) and analyzed using the Bonferroni method. The statistical significance was determined based on the P level of 0.005.

3. Test results

Figure 7 illustrates the effect of a jade extractant (distilled solution) on dopamine release response to nicotine in the striatum. After an infusion of nicotine (0.4 mg / kg, hypodermic injection), the extracellular concentration increased of dopamine gradually to peak in 40 minutes (403.9 ± 33.2% of baseline), and then decreased to return to the baslin level in 120 minutes. When 0.5 ml of jade extractant was injected intraperitoneally 60 minutes before a nicotine infusion, an increase in the extracellular concentration of dopamine in the striatum decreased to 52.0% depending on nicotine (193.9 ± 6, 1% in baselin). Injection of jade extractant also inhibited the time required to reach the maximum level of dopamine after nicotine infusion (80 minutes after nicotine injection). The jade extractant according to the present invention had no effect on the concentration of extracellular dopamine in the stable striatum.

As described above, the experimental results showed that the jade Extractant according to the present invention due to dopamine release suppressed by methamphetamine and nicotine in the striatum of the mellitus rats.

The experiment (Experimental Examples 5 and 6) served to demonstrate the effects of jade Extractant of the present invention, the space containing jade, the jade Foul water, after a precipitation of jade powder, in terms of reproduction and to study the growth in mellitus rats. The experiment was initiated by the Ko rea Food Research Institute. The details are described below.

Experimental example 5

To the relief effect of nephrite jade in relation to the symptoms of streptozo The experiment was to investigate tocine-vaccinated, diabetic mellitus rats carried out by the Korea Food Research Institute. The details are below wrote.

The thirty-two (32) Sprague-Dawley rats weighing 252-294 g became Strepto zotocin (35-40 mg, dissolved in 0.1 M citrate buffer with a pH of 4.0) intraperito neal given to induce diabetes. 7 days after administration of Streptozotocin was ba rats that showed a blood glucose level higher than 250 mg / dl based on Uropapier (Eiken Chemical Co., Ltd., Japan). 4 experimental Groups of 5 rats each were used for 50 days of an experimental phase. The rats of group A were given 1.0 ml of distilled jade water (pH 6.4) intraperitoneally  injected every day. Group B rats were housed in cages, with jade Products were placed on the floor during the experiment. Group C rats were made with jade digested water after a precipitation of jade powder as drinking water administered every day. Group D rats were injected with streptozotocin as a control only ed. Light food that was fed was conventional rat food, and the rats were maintained according to general practices. The rats were killed on the 50th day and blood was drawn from the abdominal aorta. Plasma was generated by centrifugation blood containing heparin prepared at 5000 rpm for 15 min after leaving of blood at room temperature for 30 minutes. Organs (liver, kidney) that are removed were weighed and recorded. Measurements that featured on the blood plasma were taken were HBA (plasma β-hydroxybutyrate), FFA (free fatty acids), Chole sterol, HDL cholesterol and triglycerides. The HBA content was quantified by ei ne measurement of increase in absorbance (OD) of NADH analyzed what by oxidation by β-hydroxybutyrate dehydrogenase, at 340 nm. Lipids in the blood were measured using a clinical kit (Eiken Chemical Co., Ltd., Japan) measured.

Table 12

Effects of jade on body and organ weight changes in streptozotocin-induced diabetes rats

Table 13

Effects of jade on changes in biochemical indices in streptozotocin-induced diabetes rats

p <0.05
^a β-hydroxybutyrate (ketone body)
^b Free fatty acid
^c Total cholesterol
^d triglycerides (neutral lipid)
^e High-density lipoprotein cholesterol

In terms of body weight, group C rats showed ge with jade water were fed a higher body weight than other groups whose body weight ver was reduced (B, D) or maintained (A) at the end of the experiment. The weight the kidneys are generally elevated in DM patients, and this tendency has also been increased; however, the weight of the liver was the lowest in rats treated with jade water were fed. Concentrations of blood glucose, ketone body and FFA were higher in the order of groups A, B, C and D. blood plasma cholesterol and triglyceride Concentrations had followed the same tendency (A <B <C <D) to the above para meters that are mentioned. From the result, rats with jade water (group C) showed were fed, comparable lower values of all parameters that were measured, unlike the other 2 experimental groups, and blood cholesterol and  FFA concentrations were lower in group C rats than in Kon rats troll group without any jade treatment. TC and TG concentrations were in the Order of A <B <C <D. The result of the glycemia index through a blood sugar Response after a meal changed depending on the examinations or the examiner. However, the experiment showed, although commercially mixed feed (TMR) fed without considering the glycemia index of cereals group C rats fed with jade water had an effect on the Lin hyperlipidemia, one of diabetic symptoms. There was no difference the HDL cholesterol concentration between the test groups. The administration of Jade water in rats vaccinated or administered streptozotocin showed effects in Prevent the reduction in body weight while preventing the enlargement of the Kidney or liver as well as relieving hyperlipidemia, one of diabetic Symptoms.

Experimental example 6

30 male and female Sprague-Dawley rats (9 weeks old) were each born Assigned treatment. Group A rats were housed in the cages in the Air, which contained nephrite jade in the vapor phase, were admitted and were used Tap water supplied. Group B rats were treated with jade foul water after one Precipitation of jade powder (2 mg / ml tap water) fed; however, none Steamed nephrite jade injected into the box. Group C rats formed a control group (no nephrite jade in the vapor phase and no jade water). The Rats were mated for 9 days and then the male rats were separated and sacrificed for sperm concentration and motility tests. The results are in the shown in Table 14 below.  

Table 14

Effect of jade products on the reproduction and growth of rats

From the findings, jade products and jade water treatments showed better ones Birth outcomes. Group A and B (90%) birth rates were higher than those from group C (80%), although rats from group C gave birth to a few more children. Also the time from mating to birth was shorter in group A rats than in Rats of the other 2 groups. In addition to these findings were the semen Concentration and motility were significant in both group A and group B rats better than in group C. However, the effects of treatments with both Jade products as well as with jade water at the same time were not included in this study examined. In summary, the results show that jade products and jade water Affect reproduction and growth. The groups vaccinated with jade products  were or were treated with jade water, were with regard to reproduction and Excellent growth.

Experimental example 7

This experiment served the change in the activity of rats using jade water, the nephrite jade powder used in the present invention was to check.

As shown in Fig. 8, when testing the stability characteristic, the mouse in the medicated control group started showing stability in its central nervous system 5 minutes later after having Chinese medicine, and then completely returned to the normal state 30 minutes later , The mouse in the general control group showed a wave-like change in its activity rate, which showed a decreasing tendency overall. The mouse activity rate in the yes-drinking group was similar to that of the general control group, showing an average activity rate slightly higher than that of the general control group and much higher than that of the drug group.

Experimental example 8

This experiment served to determine the effects of jade on the growth rate, the sper to check mamotility and the size of a muscular movement when the water that comes from a cleaning facility, made of nephrite jade, used in the before lying invention, available from Chuncheon, Korea, and the AIN convenience food before guesses in a normal cooling facility to which test groups (white rats) were fed.

The tests were carried out on a control group (A) fed with the water from a general cleaning facility, and the AIN (American Institute of Nutrition) foodstuffs that were stored in a conventional cooling device, ge was fed, and a jade-treated group (B) that was fed with the water, that was supplied by a jade purifier and the AIN food that is in was stored in a cooler made of jade material, was fed. 9 white rats (10 weeks old) for each test group (3 × 3 repetitions), in total  18 rats were bred, and the growth rate, the sperm motility, the spermacon concentration, blood lipid concentration and the size of the movement were checked.

Table 15

⚫a: general cage; Water from the general cleaning facility and AIN foodstuffs, stored in a conventional cooling facility;
⚫b: cage with jade-treated tile; Water from a jade purifier and AIN groceries stored in a jade cooler;
⚫p <0.05: statistically significant at a level of 95%.

Results

 2 treated groups showed no difference in the birth rate and in the blood lipid concentration as well as the growth rate.

 While there is a significant difference in sperm vitality between 2 Groups were present, the sperm concentration was that treated with jade Group about 17% higher than that of the control group.

 When measuring sperm motility, the swimming endurance was that of jade treated group about 1200 seconds on average, longer than that the control group.

 The blood pH of the group treated with jade was significantly higher than that few of the control group.

 The control group showed a much higher HDL level in the blood lipid than that group treated with jade.

As can be shown from the results above, the jade is unaffected delte group compared to the control group in swimming endurance and sperm better concentration. In particular, the group treated with jade showed significant lower levels of TC and TG than the control group.

Experimental example 9

As confirmed by Tables 16 and 17, the effects were differentiated Types of water for the birth and weaning of test groups (white rats) to investigate the tests assigned to 5 week old white rats. The drinking water was divided into 3 groups; that means groundwater (A), water from an ordinary Chen cleaning device (B) and jade distilled water of the present invention (C). As test feeds, AIN diet food was evenly administered to the test groups tert. Test groups were assigned to each of the 9 female white rats (♀), (3 × 3 Repetitions) (repetitions), a total of 27 rats, and each 8 male, white Rats (♂) were assigned to test groups, a total of 24 rats. After 4 weeks test group rats were mated for 7 days, the Paa ration ratio ♂ / ♀ = 1: 3.

After 3 weeks of pregnancy, the test group rats were born to involved in the weaning period.

Table 16

Effects of different types of water in white rats

Table 17

Effects of different types of water on the birth of white rats

Results

 There was no significant difference in the growth rate, the amount of movement and sperm motility among the treated groups, A, B and C.

The sperm concentration of the treated group C, that is, the group treated with nephrite jade distilled solution of the present invention, was 4.87 × 10 ⁸ / ml, 21.4% and 14.3% higher than the other treated groups A and B, that means 4.01 × 10 ⁸ / ml and 4.26 × 10 ⁸ / ml.

 The birth rates were 55.6%, 66.7% and 77.8% for the groundwater, the Water from the general cleaning facility and jade distilled water each, with the birth rate of group C treated with jade being significantly higher than was that of the other groups.

 The number of boys averaged 11.2 ± 1.9, 7.7 ± 2.9 and 11.6 ± 0.5 Groundwater, water from a general cleaning facility and from jade distilled water each.

 The number of boys who were breastfed 3 weeks after birth was gene 53, 39 and 69 in the treated groups A, B and C respectively, and the Ge bad (♀ / ♂) ratios were 26/27, 17/22 and 41/28, which showed that the male boys were more jade than the female boys delten group C.

From the findings, the sperm concentrations were those treated with jade Group 21.4% and 14.3% higher than those of the other groups, respectively. Were too the birth rates of the female white rats of the jade-treated group, d. H. 78%, higher than those of the other groups; that means 56% and 67% each. Furthermore, the sex ratio of the weaned boys was the male Boys in the group treated with jade were higher than those in the other groups.

Experimental example 10

The experiment was conducted to evaluate the effect of the jade treated water obtained by purifying tap water using a filter formed by nephrite jade used in the present invention the silkworm and the amount of silk produced (see Fig. 9).

(1) Materials and processes

• 1. Species of silkworm: white gemstone caterpillar (white gem silkworm).  
• 2nd growing season: November to December.
• 3. Process for growth: constant temperature and constant humidity, left during all instars.
• 4. Number of silkworms:
  Control group 150 (repetition),
  Group 130 treated (repetition),
• 5. Treatment:
  Control group: Mulberry leaves, sprayed with distilled water, were fed.
  Treated group: Mulberry leaves sprayed with water treated with jade were fed.
  Time to treat the silk worms: starting from the 2nd instar.

Table 18

Breeding results obtained with the treatment with jade powder water

Table 19

Kind of a cocoon wrap with jade powder treatment

Table 20

Change in larval weight under jade treatment

Table 21

Decreasing effects of blood glucose content through jade solution

Results

• 1. The larval weight of the group treated with jade was higher than that of the Control group in each instar. In particular, in the case of a matured larva (mature larva) the weight of the group treated with jade by 0.48 g per larva higher than that of the control group.
• 2. The pupation percentage of the group treated with jade was 71.2% was 15% lower than that of the control group. The cocoon yield with The jade-treated group was 15.4 g per 10,000 larvae, which was 7% lower was that of the control group.
• 3. The single cocoon weight of the group treated with jade was 2.14 g, which was about 6% higher than that of the control group. The knockout was also Kon-coat weight of the group treated with jade 44.5 cg. The cocoon The mantle percentage of the group treated with jade was 2% higher than that of the control group.  
• 4. The reeling performance of the group treated with jade was generally better than that of the control group. In particular, it was with jade treated group with regard to the cocoon filament length, the cocoon Filament weight and winding ability excellent (higher by about 9% than in the control group).
• 5. With regard to the silk quality, the cleanliness point was that with jade delten group slightly higher than that of the control group. They were tough too strength or tensile strength, the elongation and the raw silk percentage of that with jade treated group higher than those of the control group. However, that was Cocoon yield of the group treated with jade was lower than that of the control group per 10,000 larvae.
• 6. No significant difference in the surface structure between the control group and the group treated with jade was identified.
• 7. The crystalline structure of the jade that was used was in the form of a rod, the had a sharp point.

The following experiment (experimental examples 11 to 15) serves the effects of nephrite jade used in the present invention available from Chun cheon, korea, and jade water prepared from sprouting and that Check seed growth.

A. Component Analysis of Nephrite Jade and Jade Water

The components of Nephrit-Jade, available from Chuncheon, Korea, were analyzed using a PW1,480-X-RIV fluorescence segmental spectrometer. The analysis results showed that the nephrite-jade SiO ₂ as a main component and contained various trace elements necessary for the growth of plants.

B. Analysis of the quality of jade foul water and underground water

The quality of jade foul water (underground water from a jade mine) and Groundwater was analyzed. The analysis result showed that no special  Trace elements in the underground water of a jade mine, i. H. Jade Faulwasser, were found.

Experimental example 11 Comparison of the effects of jade foul water on the sprouting of seeds

 The experiment of sprouting was carried out so that 20 strong ones Seeds of balsam or balsam, beans, rice, radish and crown- Daisies (crown daisy) were selected from 8 sheets of toilet paper a bowl (laboratory equipment) was placed to place the seeds on it, and then jade foul water, groundwater and tap water were added.

 Seeds that have been supplied with jade fermentation water and those that have tap water were supplied, were 50 m apart and were in left in a dark place at room temperature for 5 days to or sprout rate 5 times.

 In order to examine the feed area of jade foul water, respectively 20 seeds of radish and rice supplied with jade water and with ground water, whereby the shells had seeds that were 10 m, 20 m, 30 m, 40 m and 50 m apart were spaced.

The experimental results are given in Tables 22 and 23.

Table 22

Comparison of the germination rates for jade foul water, underground water and tap water

The results convey that the jade foul water causes the germination or sprouting of the 5 types of seeds listed above accelerated.

Table 23

Comparison of the germination rates of radish seeds when they are supplied with jade fermentation water and groundwater

The results convey that the aura of jade water exerted over approximately 20 m and accelerates the sprouting or germination of radish seeds.

Experimental example 12 Comparison of effects of the aura of jade porcelain on sprouting or sprouting of seeds

The germination experiment was carried out so that 20 strong seeds of balsam, beans, Rice, radish and crown daisy (crown daisy) were selected from 8 leaves Toilet paper was placed on 5 jade porcelain and 25 bowls to hold the seeds to be placed on it so that they are 10 m, 20 m, 30 m, 40 m and 50 m apart were. Germination results were observed at room temperature to the extent determine the aura (see Table 24).

 The seeds were left alone in a dark place at room temperature for 5 days left to observe the germination rates 5 times.

 The statistical values are based on a total of (100%) 5 times.

Table 24

The results convey that the aura of jade water exerted over approximately 30 m and the sprouting of the seeds as listed above be accelerated.

Experimental example 13 Comparison of the effects of jade porcelain and jade foul water on the growth of Bean sprouts

To the effects of jade porcelain and jade foul water on the growth of plants to test were root formation experiments in bean sprouts and onions carried out.

 Each of 30 beans for bean sprouts was grown on dishes for cultivation placed by bean sprouts; then the sprout formation rates and the Growth rates compared at room temperature for 10 days, with jade Digestion water, ground water and tap water were supplied.

 Each of the 30 beans was made on jade porcelain, white porcelain and plastic containers were placed, and then the sprout formation rates and the wax growth rates at room temperature for 10 days, using jade foul water, Groundwater and tap water were supplied.

 3 bulbs of the same size were earmarked for root formation experiments, whereby jade foul water, ground water and tap water were supplied.

 Jade porcelain and jade fermented water tanks were made without trays 50 m Jade material spaced.

The experimental results are described below.

1. Growth of bean sprouts with jade porcelain

Since the aura of jade porcelain speeds up the time for sprout formation, it was confirmed that the aura of jade porcelain had the effects on the growth rate owned by bean sprouts.

2. Growth of bean sprouts with jade fermented water

As the aura of jade water accelerated the sprout time, it was confirmed that the aura of the jade water has the effects for the growth of bean Had sprouts.  

Experimental example 14 Comparison of the freshness of plants through jade porcelain and jade foul water

 The flowers of the same kind were encased in jade porcelain and white porcelain and their freshness was compared while groundwater was being fed de.

 The flowers of the same kind were put in glass bowls, freshness was compared while jade foul water, groundwater and tap water was fed.

 The jade porcelain and the jade foul water were 50 m from bowls without yes de-material spaced.

 The flowers that were used were 5 roses, carnations, chrysanthe each men, planted at the same time period, and flowers with the same inflorescence were selected for observation with the naked eye once a day.

The results are described below.

1. Jade porcelain and freshness of the plants

The aura of jade porcelain has been adjusted to easily reflect the freshness of the Plants affected.

2. Jade water and freshness of the plants

The aura of jade water has been adjusted to easily reflect the freshness of the plant zen influenced.

Experimental example 15 Effect of nephrite jade powder on plant growth

 3 seeds each from the haricot bean, peas, radish and cabbage in pots with 20 g nephrite jade powder and 50 g fine sand to compare the Plant growth rates sown.  

 Due to a difficulty in fertilization, radish and cabbage were not fertilized. However, there were seeds of the kidney bean and peas with leaf mulch and sand mixed in the same ratio.

 The pots of nephrite jade powder and the white pots were 50 m apart spaced.

The results are described below.

1. Pea growth

Because the aura of nephrite jade powder shortened the sprouting time, this went away judged that this slightly affects the growth of peas.

2. growth of haricot beans (m)

Because the aura of nephrite jade powder accelerates the sprouting time of kidney beans , this was judged in that it easily allowed the growth of the garden bean influenced.

3. Growing onions

The aura of jade water has been judged to be considerable Root formation influenced by onions.

4. Radish growth

The nephrite jade powder is judged to be the sprout time of Ret tich influenced to support the growth of radish.

As confirmed from the experimental examples described above The following conclusions can be drawn.

 Nephrite jade, available from Chuncheon, Korea, has negative values of δ 180, with hardnesses of 6 - 6.5, and the measured values of δ D of the nephrite Jade are controlled by a hydroxy group, the value of δ 18D being the Hydroxy group is considerably lower than that of the entire mineral and  is outside the range of degenerate water, that means δ D (%) = 0 ~ -70.

 The components of nephrite jade samples are different from the collection space dependent.

 The effects of the aura, created by the nephrite jade and jade porcelain when sprung and the growth of plants extends to approximately 30 m.

 The effects of the aura of jade water on the sprouting and growth of the Plants extend to approximately 20-30 m, which is slightly less than that Aura of nephrite jade is.

 The sprouting experiments showed that both jade water and Jade porcelain also supported the sprouting of seeds, although one slight difference between the control group and the treated group existing that was.

 The growth behavior of radish, peas, haricot beans and cabbage was through Nephrite jade powder supports, which leads to the assessment that acidified Water through a new fertilization process using nephrite jade or jade water can be improved for use in hydroponics. Also can nephrite jade or jade water as a neutralizer for drinking water, an ent Removal agent for concentrated heavy metals or a support agent for the growth of plants can be used.

The following experiment (experimental example 16) serves to examine the effects of Jade products (jade tile, jade slice, jade powder, jade cushioning material or the like), made of nephrite jade from a jade mine, located in Chuncheon, Korea, similar to the nephrite jade ore, which is a jade extractant of the present invention is used for the survival and reproduction of Mee to examine resmicroorganisms.

Experimental example 16 (1) Microorganism source

In this experiment, sea water, available from Daecheon, Korea, was used was mainly used to make media as the source of Marine bacteria (Marine Bacteria) in a state of aged sea water used, where most organic materials by storing the Were decomposed in a dark place for 6 months. The Mee resphotobacterium used in the experiment, that means photo bacterium Phosphorium, was a gram-negative bacterium, and the sample thereof was used which had a luminescence intensity of 1.4 × 1014 quanta / sec per showed every milliliter. The sample was diluted and approximately 1% of the diluted Sample was used for grafting. Then were to make changes of cell growth and luminescence intensity over time to check the adsorption at 660 nm and the luminescence intensity of 1 ml sample below Use of a spectrophotometer (Milton-Roy MR 3000) and a Lumino meters measured in each case.

(2) Media that was used

The media that was used included Zobell media traditionally used for Cultivating marine bacteria are used, and complete seawater Media and compositions thereof are as follows.

Zobell medium

Bactotrypton: 3 g
Yeast extract: 1 g
FeCl ₃

: 0.1 g
Aged sea water: 700 ml
Distilled water: 300 ml

Seawater complete medium

Bactotrypton: 5 g
Yeast extract: 3 g
Glycerol: 3 ml
Aged sea water: 750 ml
Distilled water: 250 ml
Petri film: 3 sts

The Petri film as the medium for which the colony forming unit (CFU) was used de, was commercially available from 3M Innovative Properties Company. 1 ml of the sample was applied to the film, pressed and cultivated for 3 days. Then the number of the red colonies, and the colony was recorded using a digital camera (Kodak DC- 120) photographed.

The jade tile, the jade disc, the jade powder, the jade ore and the jade cushion or the like used in the experiments were made from nephrite jade, available from Chuncheon, Korea (manufactured by Ocksanga Co., Ltd., Korea) on a trial basis manufactured. The respective experimental groups were assigned to each experiment net after being cultured in a 100 ml cultivation bottle. The jade powder, that was used was 1 g per 100 ml of sea water, and the jade ore that was used was 1 g. Also, the jade pillow used was 1 g in total velvet.

(3) Experimental relationships A. Change in pH

100 ml of sea water was used as a control group, and 1 g of jade powder have been added. Then the mixture was used with a magnetic stirrer Stirred for 30 minutes and centrifugation was carried out to remove the supernatant Obtain lazy (supernatant) jade water. Then the pH change was yes de-foul water measured as a function of the concentration of the jade powder.

B. Change in the number of colonies

In general, sea water was made by stirring it with sterile sea water used.

Solid medium

Petri film
Zobell plate

Liquid medium

Cultivated on a jade tile
Cultivated on a jade disc
Cultivated on jade ore

C. Change in luminescence intensity

The sample from Photobacterium Phosphoreum was washed with a seawater Complete medium is used so that the luminescence intensity is 1.4 × 1014 quanta / sec was checked for every milliliter. The sample was diluted and un About 1% of the diluted sample was used for dripping. Then, to see changes in cell growth and luminescence intensity across the Time to check the adsorption at 660 nm and the luminescence intensity of 1 ml Sample using a spectrophotometer (Milton-Roy MR 3000) and egg luminometer measured in each case.

(4) Measurement of the biomass Biomass measurement using a glowworm glow donation Lighting System

There are various methods for measuring biomass. all dings is a method for measuring the amount of ATP's (Adeonosin-5- Triphosphate), the ATP being a bio-indicator as it usually lives in all is included in things to measure the biomass of an invisible microor ganism has been used. ATP's are essential maintenance factors living organisms are stronger than that of bacteria, and are Metabolism generated by different types of organisms, called direct burning substance is used in the cells. Since the ATP quickly becomes ADP (Adeonosin-5 Diphosphate) can be broken down into dead organisms, this can be a good indicator Measure the biomass of a living organism. On the other hand, it is when the biomass is estimated by the naked eye or by a microscope,  be very difficult to live and dead organisms in the case of microorganisms differ. In the case of microorganisms, a cultivation method can be used not for measuring the biomass of a viable, but non-cultural (VBNC) cell. It can also contain less than 1% of marine organism can only be cultivated by a general cultivation method.

Since the extinction rate of living organisms on the surface of jade Products are very important in samples used in the experiment of the invention it is very important to measure the biomass accurately. An ATP Measurement was performed by extracting a sediment using a buffer accepted. Various chromatographic processes can be included high-performance liquid chromatography (high performance liquid chro matography - HPLC) or thin layer chromatography (thin layer chro matography - TLC) can be used. In this study, a bioluminescent Process for the glow worm that produces light is turned on using ATP puts. Although the HPLC or TLC method gives accuracy in the measurement result nis supplies, pretreatment of samples is very complex, which takes a lot of time and effort required, and the detection accuracy thereof is low using UV adsorption or pigmentation dependence. A beacon or Fi refly-luciferase generates light of 530 nm, while luciferin in oxyluciferin, below Use of 1 M of ATP and 1 M of oxygen, which is converted, and the Luminescence effectiveness is essentially 100%; the highest among known ones Bioluminescence systems. A measurement of the amount of ATO using the glowworm luciferase is recognized as the most sensitive process finally, the process that uses radioactive isotopes.

(5) Experimental results 5.1 Change in pH

Sea water generally shows a weak, alkaline pH, ie about 8.1, which gradually becomes neutral due to the metabolic activity of the microorganisms contained in the sea water when left intact for a long time. This is adequately defined by the control group in Table 21. In the presence of jade products, there is a slight change in pH with the addition of it, that means within 1 hour. Both jade powder and jade ore showed a change in pH, about 7.8, which is a weak, alkaline level. This tendency is weakened in the case of a jade tile that is not in direct contact with the seawater solution, which means essentially similar to that of the control group. The results indicate that materials that are released from the jade product itself into the ambient solution representatively silicon dioxide (SiO ₂ ) that changed acidity.

Table 25

Change in pH of seawater from jade products

5.2 Change in the number of colonies

100 ml of standard seawater was placed in a sterilized bottle, and Various jade products were added in predetermined quantities. The bottle containing the jade products became a kul at approximately 100 rpm Activation shaken, and 1 ml each was taken out over time then grafted using a Petri film.  The number of colonies generated after 3 days of cultivation were counted (see table 26).

Table 26

Change in the number of colonies in seawater through jade products

It can be seen from Table 26 that all jade products have a noticeable Ab showed an increase in the number of seawater bacteria. Show during an early stage ten jade pavement and jade ore have a high decrease rate. After 7 days of cultivation jade powder and jade patch showed the highest decrease rates. In particular had Jade patches the highest weight loss effect, about 64% of those of the control group, after 7 days.

5.3 Change in luminescence intensity

With regard to the change in the luminescence intensity of Photobacterium Phos Phoreum was not a significant change due to jade products included in the expe riment were used. Although the change is only 10% or was less, a decreasing tendency was shown in all samples. This shows that jade products serve to increase the activity of seawater bacteria suppress, as shown in the experiments described above.  The Photobacterium Phosphoreum is a typical bonding bacterium that easily combines with organic masses present in sea water and spreads to show luminescence, what the possibility of penetration into the inside of fish baits (see Table 27).

Table 27

Change in the luminescence intensity of Photobacterium Phosphoreum by Ja de products

5.4 Adhesion tendency

It is known that bacteria in seawater primarily on the surface of jade Adhere to products that are immersed in seawater, around a biofilm or on to form sea or marine organisms, which are sequentially affected by the action of Adhere biofilms as a basic or basal material to cause biofouling chen. Although it is not yet clear which antibiotic Features Jade products originate from antibiotic features the experimental examples described above were confirmed. As a result, can the jade products used in the present experiment  which serve to suppress the formation of a biofilm as a valuable material s can be used.

The experimental results showed that the tendency to bacterial adhesion 92-95% of those in the control group was, which reduce the adhesion showed the effect of 5-8% (see Table 28).

Table 28

Adhesion tendency of seawater bacteria on the surface of jade products (1 week cultivation)

(4) Result

As can be seen from the experimental examples described above, redu graces the injection of nephrite jade, which is for a jade extractant of the present Invention was used 10-40% of the general bacteria from the sea water what shows that the present experiment is bad to put it in a cultivation container to examine in pilot size. In particular, since there is a jade tile as the material the bottom of a cultivation container can be used, the experiment in Be pull on the initial survival rate of young fish in a 2 m container Width, 3 m width and 1 m depth.

Experimental example 17

To enhance the effect of nephrite jade used in the present invention investigate, this experiment was carried out at Bio-Information System Engineering-Laborato ry at Inha University in Korea on behalf of the applicant.

The results of the bioengineering experiment with nephrite jade will now be described become. Since the human body consists of 70% water, many relationships can between the change in water and the influences on the human body be there. That is why the effects of nephrite jade on hard water and then checked for the growth of digitalis (foxglove) plant cells.

A. Change hard water Experiment 1

50 ml of hard water (hardness: 100 ppm), man-made, were placed on 4 flasks spread each, and nephrite jade was placed in 2 of these bottles for 10 minutes.

Titration using EDTA (factor: 2.9412) was performed to determine the Determine change in hardness. The buffer solution (pH 10) of 1 ml and EBT as an indicator was used.

The change in hardness was determined from the amount of EDTA that reached the Au was used to change the color of hard water through EDTA has been. At this point the color can be reset to the original color, with time; however, the determination was made in the present experiment made at the change point.

Result 1

For 100 ppm hard water, 1.70 ml of EDTA was used.

After treatment with nephrite jade, 1.25 ml of EDTA was used. Demzufol The hardness was reduced from 100 ppm to 73.53 ppm. That means the decrease effect of hardness was 26.47%.

Experiment 2

200 ml of hard water (hardness: 100 ppm), prepared as in Experiment 1, were in a bottle was inserted and nephrite jade was immersed in it.

After 30 minutes, water filled in the bottle was opened in 3 bottles divided and the change in hardness was determined. In this experiment, EDTA Let it drip in until there was no further color change after the Color of the hard water was changed by the EDTA, making it a more precise experiment ment and the hardness at that point was measured.

Result 2

The hardness of 3 samples of hard water, the hardness of which is 100 ppm before treatment with Nephrite jade were changed to 89.62 ppm, 91.19 ppm and 89.62 ppm, respectively the average of which was 90.14 ppm. Accordingly, the decrease effect was Hardness 9.9%.

Experiment 3

This experiment was carried out with ordinary tap water. The lead water contained in a container was divided into 6 bottles, and the Ne phrit-jade was placed on the bottom of 3 bottles under the above bottles Splits. The hardness was determined after 5 minutes.

Result 3

The hardness of 3 samples with tap water that was not treated with nephrite jade , were all 97.48 ppm, and those from 3 tap water samples treated with nephrite Jade treated were all 91.19 ppm. As a result, the decrease effect was the Hardness 6.5%.

B. Effect of nephrite jade on the proliferation of suspended Plant cell suspension (Digitalis Lanata)  Effects of nephrite jade on the growth of digitalis lanata cells in the growth medium

Fig. 10 is a graph showing the change in the total volume of the medium-liquid of cell proliferation in the growth medium observed for 11 days, and Fig. 11 is a graph showing the volume changes of the digitalis Represents Lanata cells. Since depletion of ingredients in the medium and evaporation of medium occurs in proportion to an increase in the cells that grow over time, the total change in volume of the medium reflects liquid. More specifically, the humidity and temperature of the air are closely related to the evaporation of the medium, and these figures also affect the growth rate of the cell.

As shown in the drawings, the total volume of the medium liquid in the case where nephrite jade was used was gradually reduced compared to that of the control where nephrite jade was not used. When nephrite jade was used, the cell growth rate was opposite to evaporation of the medium, and a decrease in nutrients was faster than that of the control for this reason, with a rapid decrease in the medium that became impoverished might be offset. As shown in Fig. 10, which measures the volume change of packed cells only from the 4th day to the 11th day after inoculation of the cell volume, in the case of using nephrite jade, an increase was more than that in the Control case observed when using no nephrite jade. As a result, when nephrite jade was used, the rate of cell growth was faster than that when nephrite jade was not used. The results are understood to mean that a decrease in the total volume of medium liquid due to nutrient depletion and medium evaporation was offset by a rapid cell growth rate in this experiment using nephrite jade, and the rate of the total volume of the medium - Liquid, compared to the control, is slowed down. In addition, the result shows an increasing tendency of the cell volume gradually after 4 days compared to the exact volume of the overgrown cells and is understood to support the above conclusions.

The full weight and dry weight of the cells are considered the most general Index for determining the growth state of the cells measured. The The growth curve of each of the cells is generally in a sigmoidal or half moon-shaped curve, and this crescent-shaped curve is in 4 Pha sen divided: the lagging phase, in which no cell proliferation and none There is an increase in mass, and this represents an adaptation time for the medium sets; the exponential phase, which shows a steep slope because of the mass a rapid growth rate after passing the lag phase is increased; the stationary phase, in which the cell growth according to the size and the Growth reached and there is no increase in mass, and the dead phase in which the total volume or the mass of the cells is reduced because the cells due to a break due to the depletion of nutrients, a se cretion of toxic components and a saturated state of cell density are dead.

Figures 12 and 13 are results of measuring cells at full weight and dry weight. Fig. 12 shows the above-mentioned 4-phase growth curve. The group using nephrite jade shows a less rapid growth rate than the control group not using nephrite jade after 7 days later and the growth in the former tends to continue up to 10 days later while the latter in the latter is stopped and the full weight of the cells decreases. When the change in concentration of glucose where a nutrient acts as a main metabolic substrate in the medium is compared, it can be seen that cell growth in the medium according to the present invention continues at the point where the concentration of glucose 0 due to an impoverishment of such a nutrient is achieved, while in the control medium (nephrite jade is not used) the proliferation or proliferation is stopped and the cell growth rate is reduced at the point at which the nutrient is used up. This shows that nephrite jade can affect cell growth. Such a result is also shown in Fig. 13, which shows a graphical representation of a measurement of the dry weight of cells. In Fig. 13, cell growth in the medium of the present invention (nephrite jade is used) represents a faster growth rate than that in the control medium (nephrite jade is not used) 3 days later and the same appearance as in Fig. 13 Fig. 12 appears 9 days later. Therefore, the cells cultured in a growth medium that is close to nephrite jade show a good effect on cell growth compared to the control. When digitalis lanata plant cells, which have a growth limit of approximately 10 days, are cultured using nephrite jade, the upper cells maintain the stable state, with cell necrosis or cell fire not without exchange with a new medium occurs up to 10 days later and, in contrast, show cell proliferation. For these reasons, the method according to the present invention can be advantageously used for optimizing cell growth and concentration as a process for producing the useful material.

 The effect of nephrite jade on the change in pH of the medium

Fig. 14 shows the change in pH in the culture medium for cells and

Fig. 15 shows the change of the pH of the Kultermediums is by adding jade-jade powder.

In detail, Fig. 14 shows a graphical representation of the measurement of the pH change in the medium using the nephrite jade. In the control measurement, the trend of pH in the medium used in typical plant cells also appears, and the pH gradually decreases over time and then maintains a certain level. However, when nephrite jade is used, the tendency of the pH is similar to that in the control tile 7 days later; however, the pH increases bit by bit afterwards. Such a phenomenon obviously occurs in Fig. 15, which is a graphical representation of the measurement of pH change in the medium, with nephrite jade powder added directly. While the pH continuously decreases in the control medium where nephrite jade is not added, the pH of the medium where nephrite jade is added tends to maintain a certain level. It is believed that an ionic reaction due to material gradients contained in the nephrite jade could be the cause of the above result.

 The result of an analysis experiment for water quality on nephrite jade

FIGS. 16 and 17 illustrate the state of the analytical instrument and the expression of the results of the analysis are, and the data are shown in FIGS. 16 and 17 Darge sets are given for each sample and the results are shown in Tables 29 shown to 32. In Table 29, each analytical attribute (Sample 1) is measured 48 hours later after the addition of a 20 g nephrite jade lump, and there is no change in each analytical attribute, and consequently there is no influence due to nephrite Jade present.

In Table 30, each analysis attribute (Sample 2) is measured 48 hours later, after a lump of 20 g nephrite jade is placed outside the solution, and there is a slight change in each analysis attribute.

Also, in Table 32 there is no change in any analysis attribute of this analysis Experiments exist where a lump of 20 g nephrite jade outside the Lö solution (sample 4) is placed. However, in the case of sample 3, each one is data Analysis attribute versus a sample 48 hours after the addition of 20 g of nephrite jade powder have passed into the solution, shown in Table 31. As shown in Table 31, there are many changes, and these changes are as follows.

The amount of Cr, Pb, Ni and Co that are harmless to the human body are changed significantly after 48 hours. The amount of Cr before adding Nephrite jade powder, d. H. before the reaction is 45.30 mg and it goes against 0; 48 hours after the addition of nephrite jade. Pb is also complete removed from 13.76 mg to 0. The amount of Ni increases from 51.8 mg to 1.733 mg and the amount of Co also decreases from 52.69 mg to 11.94 mg. The amount von Co also decreases from 52.69 mg to 11.94 mg. The amount of Mg, one of the essential ingredients for the human body, increases by 48.36 mg  to 55.74 mg, and this results from the binding of Mg with the nephrite jade Components themselves; however, there is no change in hardness.

The hydrogen ion concentration of distilled water is acidic pH of 3.5, but changes to a neutral value with ei pH 6.8, and the conductivity decreases rapidly. The adsorptive powder is about 3-4 mmol.q (equivalent) per 1 g and is a high value in the physika meaning.

Table 29

Result of an analysis before and after the reaction (sample 1: nephrite-jade mass (20 g) in solution form)

Table 30

Result of an analysis before and after the reaction (sample 2: nephrite-jade mass (20 g) not in solution form)

Table 31

Result of an analysis before and after the reaction (sample 3: nephrite jade powder (20 g) not in solution form)

Table 32

Result of an analysis before and after the reaction (sample 4: nephrite jade powder (20 g) not in solution form)

As described above, the proliferation of Digitalis Lanata plant census pension cells that have been cultured near nephrite jade for about 1 week 30% increased compared to the same cell culture without nephrite jade. The result obtained is very surprising since the result is similar to this not in the different expe Rimenten that were previously carried out to proliferation of Digitalis Lanata-Pflan to examine cells for a culture with high concentration. additionally In the experiment of changing the water hardness, the fact was found that Nephrite jade the hard water, even without contacting the water, he softened. In particular, in the experiments of elemental analysis for distilled Water in which nephrite jade powder has been deposited or precipitated, speci All results of an increase in pH, a decrease in Ni and Co, one Removing heavy metals such as Cr and Pb and increasing MG occurred.  

Although the effect of nephrite jade (powder) used in the present invention , has not yet been clarified theoretically, the effects occur, such as changing the hardness of the water without touching the water, increasing the polife ration of digitalis lanata plant suspension cells by 30%, reduction of Ni and Co and removal of heavy metals, such as Cr and Pb, for the men are toxic, certainly due to the emission of electromagnetic waves, presented in the results of the IR study and the ionic reactions of inorganic components contained in nephrite jade. The above described Facts may be caused by effects in the products of the present invention kick, be confirmed.

Experimental example 18

This experiment is designed to measure the change in pH depending on the time when jade ore, a jade necklace, and jade powder are immersed in or added to water, which is provided by different sources, for 8 to 11 days (see Fig. 18 to 21) to be checked.

The jade materials used in the experiment were jade ores from Chuncheon, Korea (Oksanga Co., Ltd.), and from Russia, jade necklaces made from nephrite jade, available from Chuncheon, Korea and China, and jade powder, made from nephrite jade, available from Chuncheon, Korea. The water that solution of the pH was comprised of water from a general cleanser supply device, groundwater and a strongly acidic solution (pH 3.2).

Results

 If jade ore and a jade necklace or collar are in the water of one general cleaning equipment and groundwater were immersed the pH values of the jade ore and the necklace made from nephrite jade available from Chuncheon, Korea used in the present invention higher than those made in China and Russia.

 10%, 5% and 1% of jade powder became a strong acidic solution added to pH 3.2, and average pH measured for  10 days, was 8.59 ± 0.10; 8.58 ± 0.13, and 8.57 ± 0.11 each, which is a small one Difference by the percentage of jade powder that was added de, showed.

 During the measurement period the pH values of the strongly acidic solution were in the bottle treated with jade is considerably higher than in an ordinary bottle cal. As shown by the above knowledge, the jade Ore, the jade powder and the jade necklace made from nephrite jade Lich from Chuncheon, Korea, the pH compared to those in Russia and China were manufactured; however, there is no statistically significant difference limit available.

Experimental example 19

The experiment serves to measure a change in the biorhythm measured by one Radionic biofield analyzer, available under the name OMNI-SENSE, when healthy, 39 year old male subjects and 19 year old female subjects with each test a necklace with large pearls, made from nephrite jade from a nephrite jade Mine, located in Chuncheon, Korea, and nephrite jade tea were taken investigate, and 4 typical cases were analyzed. The test results are in the Tables 33 and 34 indicated.

Table 33

Jade necklace (with large pearls)

Table 34

Jade tea

As can be shown from the results above, is a big sub differed in the measured values before and after a treatment 13445 00070 552 001000280000000200012000285911333400040 0002010050424 00004 13326 with jade present. A higher value means that the jade components are more useful for human Bodies are. Although the experimental example described above in healthy, adults, there is a greater difference in the measured expected values in the case of patients. The Radionic Biofield Analyzer, which in used in this experiment is a combined analyzer of a traditional one Analog radionic biofield analyzer and a digital radionic biofield analyzer. A 64 Kbytes Pocket Com (pocket computer with 64 Kbytes) was in the radio Biofield analyzer used in this experiment. Installments of about 400 basic items to determine the physical properties levels of the human body, including balance of yin and yang, hormone balance, metabolic problem, deficiency  Vitamins, lack of minerals, sensations, and the like are in the Ta programmed computer.

The reference values of the Radionic Biofield analyzer and the measurement conditions thereof are summarized below.

Table 35

Radionic Biofield analyzer reference values

Fault tolerance: ± 5%, permitted according to an environmental factor of the measuring point and the physical condition of the person analyzing it.
Measuring range: -100% + 100%, normally in the range of 0 - 100%.
Measurement environments: EMI free space (free from electromagnetic influence), optimally a place or a place where no interference from other strong waves occurs.
Energy: Compatible for a 220 V AC adapter and built-in batteries (A4 × 4).

Experimental samples 20 and 21 are used to demonstrate medical effects of various products containing nephrite jade powder, which is described in the present invention is used.

Experimental example 20

To achieve beneficial effects of various products of personal ornaments, the Ne contain phrit-jade powder, prepared for the experimental examples of the present Invention, e.g. B. necklaces, rings, bangles, the experiment was through the Chinese Medical Center, affiliated with Deajeon University.

In men and women over 45 years old, the ordinary ornaments, ornaments, prepared according to the present invention, worn for over 2 hours, changes were made physical condition in the test subjects, and the results are in Tables 36 to 38 listed below.

Table 36

Test on 45 year old men

Table 37

Test on 55 year old men

Table 38

Test on 64 year old women

As can be seen from the results shown in Tables 36 to 38, the oxygen concentration was higher among those who wore the ornaments from Nephrite jade of the present invention were manufactured as such, the ordinary one Had ornaments. Accordingly, the ornaments of the present invention showed Drawn effects in supporting the metabolism and a smooth or disturbance free blood circulation.

Experimental example 21

Clinical trials were conducted in patients who showed multiple symptoms the patient was allowed, the bed, the room, the sofa, the chair and the like, covered with a fabric covering that was made from nephrite jade that was in the previous lying invention was used to use. The clinical test results show that 88% of patients showed symptom-reducing effects.

⊙Criteria and procedure for selecting test subjects

Among people in the Chinese Medical Center that is connected to Daejeon University who have been treated were 25 patients suffering from headache, sleep loss nausea, dizziness, discomfort or drowsiness or numbness of Hands and feet were selected for clinical testing.

⊙Observation attributes and procedures

Patients who have symptoms such as headache, insomnia, dizziness, discomfort have lightheadedness or numbness of the hands and feet, was allowed to wear the ornaments for a predetermined period (over 10 days), and then the physical states of the patients and changes were observed.

⊙Evaluation criterion and procedure

• A) Evaluation criterion: Conditions during the first medical examination for observ esteem, attributes  
• B) Evaluation Procedure: Described in Table 39 below. The results are shown in Table 40 below.

Table 39

Table 40

As can be seen from the results of Table 40 above, it is the most of the patients who have been subjected to clinical trials are better at looking  symptoms of headache, dizziness, insomnia, discomfort, Senselessness of hands and feet, indigestion or the like, gone. These results fully confirm the excellent medical results of Nephrite jade.

Experimental example 22

The test cup, prepared from nephrite jade, used in the present invention and the control dish, made of polyethylene, were made with homogenized milk Level A filled and left for 48 hours at ambient temperature. Then the milk was subjected to an analysis. The results are shown in Table 41 below shown.

Table 41

Conclusion: The number of microorganisms necessary for the human body harmless has been reduced.

In this experimental example, tests for the food decomposition rate of Rice bowls made of synthetic resin, made from nephrite jade powder, which in the front lying invention is performed, as shown in Table 42.  

Table 42

Experimental procedure

Five jade bowls (test group) and five ordinary bowls len (control group) were mixed with an equal volume of rice and sterile Filled with water and were left at room temperature for 24 hours. The two groups pens were spaced approximately one meter apart with the lids removed to expose them completely to the air. As shown in Table 43 below standard plate or plate counting tests were carried out.  

Table 43

Conclusion: When decomposing food in the shells of the test group and the control group shells were the food decomposition rates of the test group pen shells lower than those of the control group shells, showing that the yes de the present invention has a beneficial effect on a food prior processing or storage.

Experimental example 23

The experiment was carried out to determine the effect of jade bowls Nephrite jade powder used in the present invention with respect to the Examines freshness of pork. Details are described in Table 44.  

Table 44

The experiment was carried out to change the effect of nephrite jade on a to examine in the freshness of pork over time when pork in a jade bowl made of nephrite jade (Chuncheon, Korea), which is available in the ing invention was used, was kept. Pork was either in a jade bowl or in an ordinary bowl (control) for 0, 4, 7, 14 or Store at 0 ° C or 4 ° C for 21 days. One hundred grams (100 g) pork, weighted cut from a piece of ham, were placed in each container during the Drip loss, the color of the flesh, the pH, the volatile, basic nitrogen (volatile Basic Nitrogen - VBN: protein denaturation) and lipid oxidation (TBA) was measured the.

Results

 The drip loss was significantly lower for pork, which was Dish was preserved, and tended to reduce significantly with time was decorated, regardless of the storage temperature.  

 One is used as an indicator of a post-mortem change in muscle pH change was not caused by the type of container, although the pH at 4 ° C was higher than that at 0 ° C.

 The VBN value, which indicates the degree of protein denaturation, was quite high at 0 ° C, for day 14 and day 21, and was definitely low at 4 ° C in pigs meat stored in a jade bowl seen over time.

 The TBA value, which indicates the degree of lipid oxidation, became lower at Pork kept in a jade bowl regardless the storage temperature.

Experimental example 24

The experiment serves to test the effects of a jade jug that the Ge taste attributes of coffee could change if the coffee was in the jade jug is made from nephrite jade used in the present invention. The experiment was carried out by the Department of Food and Nutricition at Chung Ang University versity and the Korea Food Research Institute. The details are below described.

1. Subject of the experiment

∎To change the taste, aroma, aftertaste and / or color of Check coffee if it is in a jade jug or an ordinary jug (Control) is served.

2. Test procedure Paired preference test

∎To test the preference or preference of one sample over the other sample.
∎Test attributes: taste, aroma, aftertaste, color.

3. Sensory boards

∎20-25 seniors who major in food and nutrition at Chung Ang-University in Korea (who are experienced with panel tests (panel test)) and Had knowledge of the test procedure).

4th test period

∎ March to June 1996
∎ once a week
∎10 times in total

5. Test container and type of drink

∎ A jade jug compared to an ordinary jug: both have the same appearance
∎Drink: instant coffee

6. Sample preparation

∎ 1 teaspoon of coffee was mixed with half a cup of hot water.

7. Statistics T-test (p ≦ 0.05) Results

• 1. Flavor: Coffee that was contained in a jade pot leads to one less bitter taste (p <0.05).
• 2. Color and aftertaste: A jade jug tends to have a better color and to give an aftertaste, but statistically insignificant.
• 3. Aroma: An ordinary pot gave a better coffee aroma than a jade Kanne.

Conclusion

A jade jug seems to make coffee taste mild (which is considered statistical is significantly documented). Aftertaste and color of the coffee contained in the jade jug  was similar to or better than the one in the ordinary pot was held (not statistically significant).

The results show that the nephrite-jade distilled solution of the present invention has medical effects in the treatment of a disease and the Metabo promotes lism of the human body when used as a drink or for medical purposes Treatment is used.

As described above, according to the present invention, a nephrite Jade extractant in the form of nephrite jade ore obtained from a Ne phrit jade mine is collected, which has excellent medical effects, to prevent the pathological symptoms such as headache, Insomnia, numb feeling, indigestion or the like, by promoting blood circulation lation and metabolism.

Claims (6)

1. jade extractant for obtaining a nephrite-jade distilled solution such that nephrite jade ore pretreated by pulverizing, sorting and cleaning and sterile, distilled water in an evaporator in a mixing ver Ratio of 1: 4 is mixed, the mixture to a temperature of 100 ° C or higher is heated, the components used by the jade ores in the distilled water are eluted with the distilled water while cooking be evaporated and the steam in a distilled solution over a heat exchange is converted. 2. Jade extractant according to claim 1, wherein the jade ore is cryptocrystalline tremolite with a negative value of σ ¹⁸ O, which has the following composition: Semi-quantitative analysis of nephrite jade powder (%) silicon 34,00 magnesium 10.00 calcium 4.90 iron 0.23 aluminum 0.16 copper 0.17 cobalt 0.046 manganese 0.14 tin 0.024 beryllium 0.00072 silver 0.0013 titanium 0.0038 nickel 0.0028 chrome 0.0030 other elements 0.0000 3. Jade extractant according to claim 1, wherein a small amount of additives and pigment or other coloring to the jade extractant adds that to drinking as health (sports) ion drinks, dietary Anti-fever drinks, nutritional tonic and other carbonated drinks used becomes. 4. A method for preparing jade extractant comprising the steps of:
Pulverizing jade ore to a predetermined size, sorting and purifying it to complete a preprocessing step;
Preparing distilled water;
Mixing the pretreated jade ore and the distilled water in an evaporator in a mixing ratio of 1: 4;
Heating it to a temperature of 100 ° C or higher to allow the jade ore-containing materials eluted in the distilled water to be evaporated with the distilled water, and
Converting the steam carrying the jade ore containing materials generated in the evaporation step into a distilled solution via heat exchange. 5. The method of claim 4, wherein the jade ore is cryptocrystalline tremolite with a negative value of σ ¹⁸ O, which has the following composition: Semi-quantitative analysis of nephrite jade powder (%) silicon 34,00 magnesium 10.00 calcium 4.90 iron 0.23 aluminum 0.16 copper 0.17 cobalt 0.046 manganese 0.14 tin 0.024 beryllium 0.00072 silver 0.0013 titanium 0.0038 nickel 0.0028 chrome 0.0030 other elements 0.0000 6. The method of claim 4, wherein a small amount of additives and pigment or any other color is added to the jade extractant, that for drinking as health (sports) ion drinks, diet drinks fever, nutritional tonic and other carbonated drinks.