DD279165A1 - MEANS FOR INFLUENCING SPARKLING AND ROOTING FOR SPRING AND LAERCHE - Google Patents
MEANS FOR INFLUENCING SPARKLING AND ROOTING FOR SPRING AND LAERCHE Download PDFInfo
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- DD279165A1 DD279165A1 DD32484889A DD32484889A DD279165A1 DD 279165 A1 DD279165 A1 DD 279165A1 DD 32484889 A DD32484889 A DD 32484889A DD 32484889 A DD32484889 A DD 32484889A DD 279165 A1 DD279165 A1 DD 279165A1
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- DD
- German Democratic Republic
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- rooting
- acids
- spruce
- amino acids
- root
- Prior art date
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- Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
Abstract
In der Folge wird ein Mittel beschrieben, welches aus einer Kombination von Aminosaeuren und Phenolcarbonsaeuren besteht und bei der In-vitro-Kultivierung von Fichte und Laerche die Sprossstreckung und Wurzelbildung positiv beeinflusst. Dabei wird nicht nur eine quantitative Verbesserung der Wurzelbildung, sondern auch eine qualitative (Feinwurzelanteil) erreicht.In the following, an agent is described, which consists of a combination of amino acids and phenolcarboxylic acids and in the in vitro cultivation of spruce and larch positively influences the shoot extension and rooting. Not only a quantitative improvement of rooting, but also a qualitative (fine root share) is achieved.
Description
Ausfühi ungsbeispteleAusfühi ungsbeisptele
Vier Wochen alte aseptisch gekeimte Fichtensämlinge wurden als Hypokotylstecklinge (entfaltete Kotyledonen + 15mm Hypokoty!) 10mm tief mit dem Hypokotyl in das nachfolgend beschriebene verfestigte Nährmedium eingesetzt. Einem Basismedium nach BORNMAN (Physiol. Plant. 57 (1983) 5-16), welches in halber Konzentration Verwendung fand, wurden 20g Saccharose sowie 4,5g Agar-Agar zum Verfestigen zugesetzt. Außerdem erfolgen eine Zugabe von je 100mg/l Glutamin (GIn) und Arginin (Arg) sowie unterschiedliche Mengen Vanillin nach dem Autoklavieren der Nährmedien. Die Messung der Sproßachsenstreckung ergab folgende Werte:Four weeks old aseptically germinated spruce seedlings were used as hypocotyl cuttings (unfolded cotyledons + 15mm hypocoty!) 10mm deep with the hypocotyl in the solidified nutrient medium described below. To a BORNMAN base medium (Physiol Plant 57 (1983) 5-16), which was used at half concentration, was added 20 g of sucrose and 4.5 g of agar agar for solidification. In addition, an addition of 100 mg / l glutamine (GIn) and arginine (Arg) as well as different amounts of vanillin are carried out after autoclaving the nutrient media. The measurement of the shoot axis extension gave the following values:
Variantevariant
Sproßachsenstreckung (mm) Steigerung in %Sprooth Axial Extension (mm) Increase in%
Kontrolle (+Arg,+ GIn) 0,75Control (+ Arg, + GIn) 0.75
Kombination mit Arg, GIn + 50 mg/l Vanillin 1,52Combined with Arg, GIn + 50 mg / l vanillin 1.52
Kombination mit Arg, GIn + 25 mg/l Vanillin 1,70Combined with Arg, GIn + 25 mg / l vanillin 1.70
Kombination mit Arg, GIn+ 1 mg/l Vanillin 1,45Combined with Arg, GIn + 1 mg / l vanillin 1.45
Kombination mit Arg, GIn+ 1 mg/l p-Cumarsäure 2,02Combination with Arg, GIn + 1 mg / l p-coumaric acid 2.02
Kombination mit Arg, GIn + 25 mg/l p-Cumarsäure 3,26Combined with Arg, GIn + 25 mg / l p-coumaric acid 3.26
Kombination mit Arg, GIn + 50 mg/l p-Cumarsäure 3,32Combined with Arg, GIn + 50 mg / l p-coumaric acid 3.32
102,6 126,8 93,3 169,3 334,6 342,6102.6 126.8 93.3 169.3 334.6 342.6
Hypokotylstecklinge von Fichte wurden entsprechend Beispiel 1 beschrieben auf einem Nährmedium nach BORNMAN mit je 100mg/l Arginin und Glutamin und verschiedenen Konzentrationen von Vanillin aufgesetzt. Nach 22 Wochen Inkubation erfolgte die Bonitur der Wurzelbildung.Hypocotyl cuttings of spruce were prepared according to Example 1 on a nutrient medium according to BORNMAN with 100 mg / l arginine and glutamine and various concentrations of vanillin. After 22 weeks of incubation, the rooting was scored.
Variantevariant
Kontrollecontrol
Kombination mit Arg + GIn + 1 mg/l Vanillin Kombination mit Arg + GIn+ 10 mg/l Vanillin Kombination mit Arg + GIn + 50 mg/l VanillinCombination with Arg + GIn + 1 mg / l vanillin combination with Arg + GIn + 10 mg / l vanillin combination with Arg + GIn + 50 mg / l vanillin
Hypokotylstecklinge von vier Wochen alten aseptisch angezogenen Fichtensämlingen wurden zur Adventivknospeninduktion 24 Stunden in einer Lösung mit 1(10mg/l Kinetin (pH-Wert = 5,5) inkubiert.Hypocotyl cuttings of four week old aseptically grown spruce seedlings were incubated for adventitious bud induction in a solution containing 1 (10 mg / l kinetin (pH = 5.5) for 24 hours.
Anschließend erfolgte eine Übertiagung auf das im Beispiel 1 und 2 beschriebene Nährmedium unter Zusatz von Vanillin oder p-Cumarsäure.This was followed by a transfer to the nutrient medium described in Examples 1 and 2 with the addition of vanillin or p-coumaric acid.
20 Wochen nach Versuchsbeginn wurde die Bewurzelung bonitiert:20 weeks after the start of the test, the rooting was rated:
Variantevariant
%-Bewurzelung% -Bewurzelung
%-Pflanzen mit Feinwurzeln% Plants with fine roots
Kontrolle 14,81Control 14.81
Kombination mit Arg + GIn + 50 mg/l Vanillin 37,03Combined with Arg + GIn + 50 mg / l vanillin 37.03
Kombination mit Arg + GIn + 25 mg/l p-Cumarsäure 13,63Combination with Arg + GIn + 25 mg / l p-coumaric acid 13,63
20,00 33,0020.00 33.00
Etablierte Sproßspitzen eines Hybridlärchenklones (Larix eurolepis) wurden auf einem in Anlehnung an BOULAY (Ann. Rech. Sylvicoles, Afocel, Nangis, France 1976,84-145) modifizierten Nährmediurn mit 10"3 M Glutamin unter Zusatz der beschriebenen Substanzen kultiviert. Die Sproßlängenzunahme wurde nach 21 Tagen ermittelt:Established shoot tips of a hybrid larch clone (Larix eurolepis) were cultivated on a nutrient medium modified with reference to BOULAY (Ann. Rech. Sylvicoles, Afocel, Nangis, France 1976, 84-145) with 10 -3 M glutamine with the addition of the described substances was determined after 21 days:
Kontrollecontrol
Kombination von 10"3MGIn + 0,1 ppm p-Cumarsäure Kombination von 10"3MGIn + 1,0 ppm p-Cumarsäure Kombination von 10"3MGIn +10ppm p-Cumarsäure Kombination von 10"3MGIn + 1 ppm VanillinCombination of 10 " 3 MGIn + 0.1 ppm p-coumaric acid combination of 10" 3 mgIn + 1.0 ppm p-coumaric acid combination of 10 " 3 mgIn + 10ppm p-coumaric acid combination of 10" 3 mgIn + 1 ppm vanillin
Sproßlängenzunahme (mm)Sprout length increase (mm)
Claims (1)
Priority Applications (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
DD32484889A DD279165A1 (en) | 1989-01-05 | 1989-01-05 | MEANS FOR INFLUENCING SPARKLING AND ROOTING FOR SPRING AND LAERCHE |
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
DD32484889A DD279165A1 (en) | 1989-01-05 | 1989-01-05 | MEANS FOR INFLUENCING SPARKLING AND ROOTING FOR SPRING AND LAERCHE |
Publications (1)
Publication Number | Publication Date |
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DD279165A1 true DD279165A1 (en) | 1990-05-30 |
Family
ID=5606504
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
DD32484889A DD279165A1 (en) | 1989-01-05 | 1989-01-05 | MEANS FOR INFLUENCING SPARKLING AND ROOTING FOR SPRING AND LAERCHE |
Country Status (1)
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DD (1) | DD279165A1 (en) |
Cited By (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
EP2229054A1 (en) * | 2007-12-20 | 2010-09-22 | Swetree Technologies Ab | Use of a fertilizer containing l-amino acid for improving root growth and growth of mycorrhiza |
-
1989
- 1989-01-05 DD DD32484889A patent/DD279165A1/en unknown
Cited By (4)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
EP2229054A1 (en) * | 2007-12-20 | 2010-09-22 | Swetree Technologies Ab | Use of a fertilizer containing l-amino acid for improving root growth and growth of mycorrhiza |
JP2011507788A (en) * | 2007-12-20 | 2011-03-10 | スヴェトリー・テクノロジーズ・アーベー | Use of fertilizers containing L-amino acids to enhance root growth and mycorrhiza growth |
EP2229054A4 (en) * | 2007-12-20 | 2013-03-06 | Swetree Technologies Ab | Use of a fertilizer containing l-amino acid for improving root growth and growth of mycorrhiza |
US9481610B2 (en) | 2007-12-20 | 2016-11-01 | Swetree Technologies Ab | Use of a fertilizer containing L-amino acid for improving root growth and growth of mycorrhiza |
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