DD271641A1 - PROCESS FOR THE ISOLATION OF PREGNANT MARE'S SERUM GONADOTROPHINE PMSG - Google Patents

PROCESS FOR THE ISOLATION OF PREGNANT MARE'S SERUM GONADOTROPHINE PMSG Download PDF

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Publication number
DD271641A1
DD271641A1 DD31520188A DD31520188A DD271641A1 DD 271641 A1 DD271641 A1 DD 271641A1 DD 31520188 A DD31520188 A DD 31520188A DD 31520188 A DD31520188 A DD 31520188A DD 271641 A1 DD271641 A1 DD 271641A1
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DD
German Democratic Republic
Prior art keywords
pmsg
serum
isolation
umd
pregnant
Prior art date
Application number
DD31520188A
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German (de)
Inventor
Helmut Fiebig
Jost Bergfeld
Ingrid Behn
Undine Hommel
Dieter Urbaneck
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Dessau Tornau Impfstoffwerk
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Publication date
Application filed by Dessau Tornau Impfstoffwerk filed Critical Dessau Tornau Impfstoffwerk
Priority to DD31520188A priority Critical patent/DD271641A1/en
Publication of DD271641A1 publication Critical patent/DD271641A1/en

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Abstract

Die Erfindung betrifft ein Verfahren zur Isolierung von Pregnant Mares Serum Gonadotrophin (PMSG). Das Verfahren hat das Ziel, in leicht beherrschbarer Weise die kostenguenstige Produktion groesserer Mengen von hochreinem PMSG aus dem Serum traechtiger Stuten zu ermoeglichen. Die Aufgabe wird durch den Einsatz der monoklonalen Antikoerper BL-PMSG/1 bis 2, insbesondere durch die Verwendung des monoklonalen Antikoerpers BL-PMSG/1, als bindungsvermittelnde Substanzen in affinitaetschromatographischen Verfahren geloest.The invention relates to a process for the isolation of Pregnant Mares Serum Gonadotrophin (PMSG). The aim of the method is to make it possible, in an easily controllable manner, to produce more cheaply larger quantities of high-purity PMSG from the serum of pregnant mares. The object is achieved by the use of monoclonal antibodies BL-PMSG / 1 to 2, in particular by the use of the monoclonal antibody BL-PMSG / 1, as binding-mediating substances in affinitaetschromatographic method.

Description

-2- 271-2- 271

Gewinnung von PM8G au· 8<t«t*r»ser<umRecovery of PM8G to 8 <t «t * r» ser <

1, Präparation des Immoblllilerten monoklonailen Antlkö r,per« 1KX>img de· fnonoklonailen Antikörpers werden null iOml CNiBf* aktiv!«"t»r Sttpihttroae »ur ReekHon gebracht, Niach der Haaiktlon wird daj AMInHetigeil laut Hei«tellervor*chrlft gewaichen, «och vorh an den« reaktive Qruippen blockiert und mit dem Wa «cn puffo r »qtiJilbfler t.1, Preparation of the immobilized monoclonal antibody, perc. 1 of the immunoconclonal antibody, is brought to zero, 0% of its active substance, and then reintroduced according to the instructions of the radiologist; "Blocked by the" reactive taps, and blocked with the watt puffo "qtiJilbfler t.

2. Da« so prÄpailerte Afilnltitigel wJrd Mit 34 Stutensaruwi \n ReakMon gebracht Dabei kenn da« Serum langsam über da« QeI1 welches In eine SHuIe gepackt wurde, gepuimipt werden oder die Belaiduing dos Qe)Isewlgt Im ßetch-Verfehren. Nach der Beladung wird da« Geil vom Ffemdsuibstainzen irelge*« sehen. Die eiutlon dies PM1SG ar folgt mit Eselgiflure/Ammonacetat· Lösung I1M 3,0-3,5,1 mof/Φ), Dan«irlhailtenetoochrelne PMSQ wind Im einen neulralon Puffer ütoerlüthrt.2. Since "so With 34 Stutensaruwi \ brought prÄpailerte Afilnltitigel wJrd n ReakMon this case since characteristic" serum slowly because "ei 1 which was packed in a SHuIe be gepuimipt or Belaiduing dos Qe) Isewlgt In ßetch-Verfehren. After loading, you will see "Geil vom Ffemdsuibstainzen irelge *". The eiutlon this PM 1 SG ar follows with donkeygiflure / ammonium acetate · solution I 1 M 3.0-3.5, 1 mof / Φ), Dan h hailtenetoochrelne PMSQ wind in a neulralon buffer ütoerlüthrt.

Claims (2)

Werfaihirem .zur teoillleiruing von iPiregmaint M aire's Semm Qtoinadiotrapihilin (IPMSQ))Werfaihirem .to teoillleiruing of iPiregmaint M aire's Semm Qtoinadiotrapihilin (IPMSQ)) bisto 2 (ZIiM 0272 umd 02711 boworaugt der monaMonato Antikörper BL-PMSG/1« am feste Träigieirw>a*eirliailiiiein getbyindiein« als sipeüiiifilscih bindende itamipoiiwrotein füir <#θ2 (ZiiM 0272 umd 02711 the monaMonato antibody BL-PMSG / 1 "on the solid triglyceride contains a" eirliailii in getbyindie "as sipeüiiifilscih binding itamipoiiwrotein for <# θ Öle Erfindung betrifft ein Verf aihren zurGewinnung von Pregnant Mare's Serum Gomadotroiphln (MSO) au« dem Serum von trächtigen Stuten. Dieses Hormon «Ad in der WetertafiirmedMn ta »atomem biotechnischer vieriaihren .tut Bruntt- umdOils invention relates to a method of obtaining Pregnant Mare's Serum Gomadotrope (MSO) from the serum of pregnant mares. This hormone, "Ad in the WetertafiirmedMn ta" atomic biotechnical fouries, does Bruntt- umd Gh a reMerlileru η ο der bekaminrten technischen Lö tunigienGh a reileril η ο of the famous technical solutions Zur Qewlnnuing von gereinigten oder »ragerelcherten PM'SQ-PrApair.aiten vwerden verichledeme Fraiktlonlerungiimethoden elngeseui, die auf iFIlitongtscMMon umd ikonwentianellen Ohiorimaitographileimethoden beruhen, Gegenüber den k'la«ilechen Verfahren, die auf veiwchledenewFllungem sowie lonenauit«For the purification of purified or agglomerated PM'SQ-PrApair.aites, certain types of fractions based on nitrate-ion-monomewith the use of iconwentianal-animaimetric methods are compared with the k'laile method, which is applied to defrosting and ionizing. weisen afinltitschiromatogrftphliche Verfa*>f.en unter Verwendumg ikonvenitloinöMer poly/klomaler Antlkönper Vorteile auf, wie höhere^nrelcherumg des PMSG giegenfliber iFremdproiteiliivera, schomemdoro Prftparallonsbedlmgiumgien urad dawilt bessere Erhaltumg dflr biologkchen Elgienschafiten des PMSiQ.For example, in the case of use of a conventyl chloride / polyclonal antagonist, there are advantages in the use of PMSG, such as the higher permeation of PMSG, and the better preservation of the biological properties of PMSiQ. Anitlköriper besteht da^ln, daß die Gewlnnuing der goolgweten Amitlköripeir uinitremmteair mit den generelilen NaehteHen vonAnitlköriper consists in the fact that the growth of the goolgweten Amitlköripeir uinitremmteair with the generalist NaehteHen of zJB.hilinsilc'hiichdorSipezlfl«zJB.hilinsilc'hiichdorSipezlfl " nfichs-ten sowie sehr aufwendige ¥erfaihren zur iBnieiliuinig der 'MoinospezWiiÄ. Olese iNactoelte wenden noch gr*v!e riwdier, woran mam berücksichtigt, daß aufgrund der !begrenzten V'erweimdniiniisdiaiuier des aiiiiiinilitäitschir.omiategir.aipihlec'hoin Reagens' die.nfichs-th and very elaborate ¥ erfahren to iBnieiliuinig the 'MoinospezWiiÄ. Olese iNactoelte apply even more greatly, which takes into account the fact that, due to the limited amount of reagents used in the aiiiiiinilitäitir.omiategir.aipihlec'hoin reagent. MonoWonaile Antiköriper, die von peTmuameint wachseinden und pr.odwzJer.enden Wytoildomen «ezwmlieil wenden, ete*ien ta praktisch ausreichander Menge und über einen tiheope-Msein unibegireniten ZeUfauim zur Veflüigiuing, so daß »leih auf IhrerMonoWonaile antibodies, which grow from petermameint and turn to protozoan monstrosities, have been practically sufficient in abundance, and have passed through a tiheope-myn unibegeden cellulite, so that on your Die EriFindu ng tat des Ziel, ein zelteparendes, kostengiinstilges, echoine ndee und eimfach zu ikamAalbemdies Vwf mhiem zur Gewinnung von PMSG aius dem Serum trachiilger Stuiten »niuigietoem.The invention did the goal, a tent-saving, cost-effective, echoine idea, and in a simple way for the purpose of obtaining PMSG at the serum of a tracheal stucco. Dafilegung des Wesens 'der iErflnd#ngExposure of the essence of the invention Der Erfiindu ng liegt die Auigabe zuigirumde, eim nachwoizilöh ibiames VGriaihron zur AimiinMuing von PlMS(G aws Sojfum oder Plasma won tröchitig* η Stuiem zu eralwickeiln, welches nach dem ΡιηΙιηφ der AWiniMlftchpemaitogr.aipihte aribelM um J The invention is subject to the proposal to develop a posthumous ibiame Vgriaihron for the purpose of AimiinMing of PlMS (G aws Sojfum or Plasma won stöchiig * η Stuiem, which after the ΡιηΙιηφ of the AWiniMlftchpemaitogr.aipihte aribelM um J Als TrägerOTaitefiaMeineignen sich alle Gubstainzein, die sich ailsfesitpthaae^^The carriers OtaitefiaMany are all Gubstainzein, which are ailsfesitpthaae ^^ oder utolhprodiulkte des PMSG werfen nniiit dem träigtenfi-xterten «loinoikteirailew Anit*§nperor utilities of the PMSG do not throw the tri-fii-xterten «loinoikteirailew Anit * §nper wird diieser Kompilex von BegileHau bs t a nzen freig ewasch em. list die W/aschiflässjgikeiiit von iieglbiitlkoffinpameiiiitein ff ei, was duir<ch Analy se gepraft wird, wird das geb um deine PMSG schonend desoribiert. Zw iDe soripititen dienen ulbMche lEMemsMiitoell, die dieThis compilex will be released by BegileHau. If you do not want to know what to do, you will be desorbed by your PMSG. Twelve soripitites are used by lememsmiitoell, who are the Das öliuiierte meSG wird danach In «!nein Pufiier überfülhirt, ta dem es ölber illnpiir<e ZaIt iMtiair isit.The oiled MESG is then over-perfused with Pufier, for which it is ill-conceived by Zait iMtiair. riBi-PMS'G/luindisimdilmZIMuira^^Ribi PMS'G / luindisimdilmZIMuira ^^ VM 4m Hyferiidomen iH-iBl-PMStG/1 und 2 sezenniiert. Öle iHybridome sfad an der SeMlom iiiiowlssenichafton der iKaiM-Marx-Uiniversitäit zugängiliicb, VM 4m hyferiidomas iH-iBl-PMStG / 1 and 2 szened. IHybridome sfad at the SeMlom iiiiowlssenichafton of the iKaiM-Marx University,
DD31520188A 1988-04-29 1988-04-29 PROCESS FOR THE ISOLATION OF PREGNANT MARE'S SERUM GONADOTROPHINE PMSG DD271641A1 (en)

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Application Number Priority Date Filing Date Title
DD31520188A DD271641A1 (en) 1988-04-29 1988-04-29 PROCESS FOR THE ISOLATION OF PREGNANT MARE'S SERUM GONADOTROPHINE PMSG

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DD31520188A DD271641A1 (en) 1988-04-29 1988-04-29 PROCESS FOR THE ISOLATION OF PREGNANT MARE'S SERUM GONADOTROPHINE PMSG

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DD271641A1 true DD271641A1 (en) 1989-09-13

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Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN102876634A (en) * 2012-09-19 2013-01-16 中国农业大学 PMSG (pregnant mare serum gonadotropin) double-antibody sandwich ELISA (enzyme-linked immunosorbent assay) kit
CN102924586A (en) * 2012-09-28 2013-02-13 内蒙古博格农牧业开发有限责任公司 Method for producing pregnant mare serum gonactotropin by monoclonal antibody technology

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN102876634A (en) * 2012-09-19 2013-01-16 中国农业大学 PMSG (pregnant mare serum gonadotropin) double-antibody sandwich ELISA (enzyme-linked immunosorbent assay) kit
CN102924586A (en) * 2012-09-28 2013-02-13 内蒙古博格农牧业开发有限责任公司 Method for producing pregnant mare serum gonactotropin by monoclonal antibody technology

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