DD227029A3 - Enzyme electrode for glucose measurement - Google Patents

Enzyme electrode for glucose measurement Download PDF

Info

Publication number
DD227029A3
DD227029A3 DD23981582A DD23981582A DD227029A3 DD 227029 A3 DD227029 A3 DD 227029A3 DD 23981582 A DD23981582 A DD 23981582A DD 23981582 A DD23981582 A DD 23981582A DD 227029 A3 DD227029 A3 DD 227029A3
Authority
DD
German Democratic Republic
Prior art keywords
glucose
enzyme
membrane
invention
electrode
Prior art date
Application number
DD23981582A
Other languages
German (de)
Inventor
Peter Abel
Original Assignee
Zentralinst F Diabetiker G Kat
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Zentralinst F Diabetiker G Kat filed Critical Zentralinst F Diabetiker G Kat
Priority to DD23981582A priority Critical patent/DD227029A3/en
Publication of DD227029A3 publication Critical patent/DD227029A3/en

Links

Abstract

Die Erfindung betrifft eine Enzymelektrode als Messwertgeber fuer Glukosemessungen, insbesondere bei hoher Glukosekonzentration. The invention relates to an enzyme electrode sensor for glucose measurements, in particular under a high glucose concentration. Ziel der Erfindung ist eine miniaturisierte Enzymelektrode zur Bestimmung der Glukosekonzentration ohne vorherige Verduennungsschritte fuer implantierbare Glukosekontrollierte Insulininfusionseinrichtungen. The aim of the invention is a miniaturized enzyme electrode for the determination of glucose concentration without prior Verduennungsschritte for implantable glucose-controlled insulin infusion devices. Die Aufgabe der Erfindung besteht darin, den linearen Messbereich der bekannten Enzymelektrode zu erweitern. The object of the invention is to extend the linear range of the known enzyme electrode. Dies erfolgt erfindungsgemaess durch die Anwendung der kombinierten hydrophoben/hydrophilen Trennmembran 3; This is done by applying the erfindungsgemaess combined hydrophobic / hydrophilic separation membrane 3; 6 zwischen Probe und Enzymschicht 5 der Sandwich-Membran. 6 between the sample and the enzyme layer 5 of the sandwich membrane. Die durch diese Membrangestaltung moegliche Realisierung unterschiedlich grosser Diffusionsflaechen fuer Glukose und Sauerstoff bewirkt, dass die Glukosediffusion in der Weise limitiert wird, dass auch bei maximaler Glukosekonzentration in der Blutprobe immer Sauerstoffueberschuss in der Reaktionsschicht 5 vorhanden ist, so dass die gesamte, in die Reaktionsschicht diffundierte Glukose vollstaendig umgesetzt wird. The possible through this membrane design implementation of different sized Diffusionsflaechen for glucose and oxygen causes the glucose diffusion is limited in such a way that even at the maximum concentration of glucose in the blood sample an excess of oxygen in the reaction layer 5 is always present, so that the entire diffused into the reaction layer glucose is converted completely. Anwendungsgebiet ist die kuenstliche B-Zelle sowie Glukosebestimmung in klinischen und klinisch-chemischen Laboratorien. Of application is the artificial B cell and glucose determination in clinical and clinical chemistry laboratories. Fig. 1 Fig. 1

Description

-3- 39815 -3- 39815

Ausführungsbeispiel embodiment

Die Erfindung soll anhand eines Ausführungsbeispieles gemäß Fig. 1 und Fig. 2 näher erläutert werden. The invention will be explained in more detail using an exemplary embodiment according to FIG. 1 and FIG. 2. Dabei stellen dar: Fig. 1: Enzymelektrode mit großem linarem Meßbereich 1 Figure:: dar thereby provide. Enzyme electrode with a large measuring range linarem

Fig. 2: Anordnung der Enzymelektrode zur Variation der Grenzen des-linearen Meßbereiches Die Enzymelektrode zur Glukosemessung, insbesondere bei hoher Blutglukosekonzentration, besteht aus der Clark-Elektrode 1 mit der Sandwich-Membrananordnung 2, die aus der hydrophoben Membran 3 und der hydrophilen Membran 4 besteht. Fig. 2: arrangement of the enzyme electrode to vary the limits of the linear measuring range The enzyme electrode for glucose measurement, particularly for high blood glucose concentration, consists of the Clark electrode 1 with the sandwich membrane assembly 2, consisting of the hydrophobic membrane 3 and the hydrophilic membrane 4 consists. Zwischen diesen beiden Membranen 3, 4 ist an einen Träger fixiert das Enzym 5, Glukoseoxidase, zwischengelagert. Between these two membranes 3, 4 is fixed to a support, the enzyme 5, glucose oxidase, stored temporarily. Die hydrophobe, gaspermeable Membran 3 ist erfindungsgemäß perforiert. The hydrophobic, gas-permeable membrane 3 is perforated according to the invention. Als Material für diese Membran eignet sich Teflon, Polyäthylen ua Gegen das unbeabsichtigte Abspülen des in der betriebsfähigen Enzymelektrode unter der perforierten hydrophoben Membran 3 gelagerten Enzyms 5, z. As the material of this membrane Teflon, polyethylene, among others supported against the accidental rinsing the operative in the enzyme electrode under the perforated hydrophobic membrane 3 is suitable enzyme 5, z. B. Glukoseoxidase, ist die Perforation enzymseitig mittels der flächenmäßig sehr kleinen hydrophilen Membran 6, z. B. Glucose oxidase is the enzyme-side by means of perforation of the terms of area very small hydrophilic membrane 6, z. B. Zelluloseazetat, die als Dialysiermembran dient, verschlossen. As cellulose acetate, which is used as a dialyzing membrane, closed. Gleichzeitig verhindert die hydrophile Membran 6 das Eindringen von störenden Substanzen aus der Blutprobe durch die Perforation in die Reaktionsschicht, das Enzym 5, und garantiert somit die unerläßliche Schutzfunktion für das Enzym. At the same time the hydrophilic membrane 6 prevents the penetration of interfering substances from the blood sample through the perforation in the reaction layer, the enzyme 5, and thus guarantees the essential protection function for the enzyme. Durch die erfindungsgemäße Kombination von hydrophober und hydrophiler Membran 3, 6 ist das Verhältnis der Flächen für die Sauerstoff- und Glukosediffusion aus der Blutprobe definiert einstellbar. The inventive combination of hydrophobic and hydrophilic membrane 3, 6, the ratio of the areas for the oxygen and glucose diffusion from the blood sample is defined adjustable. Es wird erreicht, daß die Glukosediffusion bei ungehinderter Sauerstoffdiffusion erfindungsgemäß in dem Maße limitiert wird, wie es zur Realisierung eines stets vollkommenen Glukoseumsatzes in der Reaktionsschicht, der Enzymschicht 5, des Glukosesensors erforderlich ist. It is achieved that the glucose diffusion is limited according to the invention to the extent at free diffusion of oxygen, as required for realizing an always perfect glucose turnover in the reaction layer, the enzyme layer 5, the glucose sensor.

Weiterhin läßt sich die Grenze des linearen Meßbereiches durch definierte Veränderung der geometrischen Zuordnung des Glukosediffusionskanals zur unter der Sandwich-Membran 2 und im Elektrolyt KCL 7 angeordneten Elektrodensystem gemäß Fig. 2 zuverlässig variieren. Furthermore, the limit of the linear measuring range can be varied reliably defined variation of the geometrical assignment of the glucose diffusion to the channel of the sandwich membrane 2 and in the electrolyte KCL 7 arranged electrode system according to FIG. 2. Dieser Effekt wird dadurch hervorgerufen, daß bei entsprechender exzentrischer Anordnung des Glukosediffusionskanales zum Elektrodensystem ein Teil der ohnehin schon in der Diffusion durch Membran 6 limitierten Glukose seitlich neben der Arbeitselektrode, der Anode der Enzymelektrode, in der Enzymschicht 5 umgesetzt wird und das dabei entstehende H 2 O 2 umgehend zerfällt, ohne vorher einen Beitrag zum Sensorsignal zu liefern. This effect is caused by the fact that in a corresponding eccentric arrangement of the glucose diffusion channel to the electrode system, a part of the already limited even in the diffusion through membrane 6 glucose laterally next to the working electrode, the anode of the enzyme electrode is converted into the enzyme layer 5 and the resultant H 2 O 2 immediately decomposes without first to contribute to the sensor signal.

Claims (2)

  1. -2- 39815 -2- 39815
    Erfindungsansprüche: Invention claims:
    1. Enzymelektrode zur Glukosemessung, insbesondere bei hoher Glukosekonzentration, gekennzeichnet dadurch, daß zur Erweiterung ihres linearen Meßbereiches zentrisch über den Elektrodenanschlüssen in die hydrophobe Membran (3) der Sandwich-Membran (2) mit eingebettetem Enzym (5) eine Perforation, deren Größe die Steilheit der Eichkurve der Enzymelektrode variabel gestaltet, angeordnet ist und die Perforation der hydrophoben Membran (3) durch eine zusätzliche hydrophile Membran (6) verschlossen ist. 1. Enzyme electrode for glucose measurement, in particular under a high glucose concentration, characterized in that the extension of their linear measuring range centric perforation over the electrode terminals in the hydrophobic membrane (3) of the sandwich diaphragm (2) with embedded enzyme (5), the size of the slope of the calibration curve of the enzyme electrode made variable, is arranged and the perforations of the hydrophobic membrane (3) by an additional hydrophilic membrane (6) is closed.
  2. 2. Enzymelektrode nach Punkt 1, gekennzeichnet dadurch, daß zur Variation der Grenzen ihres linearen Meßbereiches die Perforation in der hydrophoben Membran der Sandwich-Membran exzentrisch über den Elektrodenanschlüssen anzuordnen ist. 2. Enzyme electrode according to item 1, characterized in that for variation of the limits of their linear measuring range, the perforation is to be arranged in the hydrophobic membrane of the sandwich diaphragm eccentrically on the electrode terminals.
    Hierzu 1 Seite Zeichnungen For this purpose, 1 page drawings
    Anwendungsgebiet der Erfindung Field of the Invention
    Die Erfindung betrifft eine Enzymelektrode zur Glukosemessung, insbesondere bei hoher Glukosekonzentration, und ist besonders geeignet als Glukosesensor bei implantierbaren künstlichen Beta-Zellen, deren Aufgabe es ist, bei Diabetikern stetig die Glukosekonzentration im Blut zu kontrollieren und mittels eines elektronischen Regelungssystems automatisch die bedarfsgerechte Insulinmenge zur Verhütung von diabetischen Komplikationen, wie z. The invention relates to an enzyme electrode for glucose measurement, in particular under a high glucose concentration, and is particularly suitable as a glucose sensor in implantable artificial beta cell, whose task is to continuously control in diabetics, the glucose concentration in the blood and automatically by means of an electronic control system, the demand amount of insulin to prevention of diabetic complications such. B. Hypo- und Hyperglykämien, zu injizieren. inject as hypo- and hyperglycaemia. Weitere Anwendungsgebiete sind glukosekontrollierte Therapiemaßnahmen, alle Situationen der kontinuierlichen oder quasikontinuierlichen Blutglukoseüberwachung und Glukosebestimmungsmethoden in klinischen und klinisch-chemischen Laboratorien. Other applications include glucose controlled therapeutic measures, all situations of continuous or quasi-continuous blood glucose monitoring and glucose determination methods in clinical and clinical chemistry laboratories.
    Charakteristik der bekannten technischen Lösungen Characteristic of the known technical solutions
    Eine Enzymelektrode zur Bestimmung von Glukose mit einem elektrochemischen Sensor, der zusammen mit einer semipermeablen Membran einen Bereich begrenzt, in dem der Akzeptor in teilweise ungelöster Form und das Enzym vorliegt, ist in der Patentschrift DE-AS 2243962 beschrieben worden. An enzyme electrode for the determination of glucose with an electrochemical sensor, which delimits, together with a semipermeable membrane has an area in which the acceptor is present in a partially insoluble form, and the enzyme has been described in the patent specification DE-AS 2243962nd
    Durch K. !to, K. Ohta et al., Comparative studies of glucose sensors between enzyme membrane and electrochemical aspects, in Artifical Organs, Vol. 2, 1978, S. 244-246 und K. ltoh, K. Ohta, T. Kondo et al.. Glucose sensors for the implantable artifical Beta-cell, in Proceedings of the International Conference in Cybernetic and Assiety, 1978, Vol. 1, S. 145-148 ist weiterhin der Aufbau von Enzymelektroden als Glukosesensoren bekannt geworden, die so gestaltet sind, daß der eigentlichen Clark-Elektrode eine sogenannte Sandwich-Membran, bestehend aus einer hydrophilen, glukosepermeablen und einer hydrophoben, gaspermeablen Membran oder einer weiteren hydrophilen, H 2 O 2 -permeablen Membran mit zwischengelagertem, an einen Träger fixierten Enzym GOD, vorgelagert ist. By K.! To, K. Ohta et al., Comparative studies of glucose sensor between enzyme membrane and electrochemical aspects in Artificial organ, Vol. 2, 1978, pp 244-246 and K. ltoh, K. Ohta, T . Kondo et al .. glucose sensor for the implantable artifical beta-cell, in Proceedings of the International Conference in Cybernetic and Assiety, 1978, Vol. 1, pp 145-148 remains the development of enzyme electrodes become known as glucose sensors are designed so that the actual Clark electrode, a so-called sandwich membrane consisting of a hydrophilic glukosepermeablen and a hydrophobic, gas-permeable membrane or another hydrophilic, H 2 O 2 -permeable membrane with zwischengelagertem, fixed to a support enzyme GOD, is upstream. Durch die hydrophile Membran, die die Enzymschicht von der Blutprobe trennt, diffundieren Glukose und Sauerstoff und zwar in dem Maße, daß die in die Reaktionsschicht GOD gelangende Glukose nicht in jedem Fall vollständig umgesetzt werden kann. Through the hydrophilic membrane separating the enzyme layer of the blood sample, glucose and oxygen diffuse and to the extent that the passing into the reaction layer GOD glucose can not be completely reacted in each case.
    Der Nachteil dieser bekannten Enzymelektroden besteht in der nicht eindeutigen Zuordnung der Glukosekonzentration zum Sensorsignal bei Überschreitung der Grenzen des linearen Meßbereiches aufgrund des relativen Sauerstoffmangels. The disadvantage of this known enzyme electrodes is the non-unique assignment of the glucose concentration to the sensor signal in excess of the limits of the linear measurement range due to the relative lack of oxygen. Da diese Enzymelektroden ausschließlich im verdünnten Vollblut arbeiten können, ist ihr Einsatz auf bettseitige Blutglukosemeßplätze bzw. Laboranordnungen beschränkt und somit nur teilweise in extrakorporalen Beta-Zellen verwendbar. Since this enzyme electrodes can work exclusively in the diluted whole blood, their use is limited to bedside Blutglukosemeßplätze or laboratory arrangements and therefore only partially usable in extracorporeal beta cells. Der enge begrenzte lineare Meßbereich beinhaltet zwangsläufig, daß zur Gewinnung des verdünnten Vollblutes dem Probanden kontinuierlich bzw. quasikontinuierlich Blut entnommen, für die Glukosemessung aufbereitet und nach der Messung verworfen werden muß. The narrow limited linear range inevitably involves that taken for the recovery of the diluted whole blood to the subject continuously or quasi-continuously blood, prepared for the glucose measurement and must be discarded after measurement. Weiterhin ist die Verdünnung der Blutprobe mit einem beträchtlichen apparativen Aufwand verbunden. Furthermore, the dilution of the blood sample is associated with a considerable expenditure on apparatus.
    Ziel der Erfindung OBJECT OF THE INVENTION
    Das Ziel der Erfindung ist eine Enzymelektrode für Glukosemessungen, die geeignet ist, auch bei hoher Glukosekonzentration und in miniaturisierter Ausführung mit optimaler Zuverlässigkeit zu arbeiten und somit den Anforderungen an eine implantierbare Glukosemeßeinrichtung zu genügen. The object of the invention is an enzyme electrode for glucose measurements, which is capable of operating even at high glucose concentration and in miniaturized version with optimum reliability and thus meet the requirements for an implantable Glukosemeßeinrichtung.
    Darlegung des Wesens der Erfindung Principle of the invention
    Der Erfindung liegt somit die Aufgabe zugrunde, den linearen Meßbereich der Enzymelektrode hinsichtlich des Verhältnisses zwischen Sensorstrom und Glukosekonzentration in dem Maße zu erweitern, daß beliebige im Individuum auftretende Blutglukosekonzentrationen ohne vorherige Verdünnung des Blutes kontinuierlich gemessen werden können. The invention is thus based on the object to extend the linear range of the enzyme electrode in terms of the relationship between the sensor current and the glucose concentration to the extent that any occurring in the individual blood glucose concentrations can be measured continuously without prior dilution of the blood. Erfindungsgemäß wird die Aufgabe dadurch gelöst, daß bei der Enzymelektrode anstelle der bekannten hydrophilen Trennmembran zwischen Reaktionsschicht der Sandwich-Membran und Blutprobe des Individuums eine gasdurchlässige, hydrophobe Membran angeordnet wird, die in definiertem Maße perforiert ist. According to the invention, the object is achieved in that a gas-permeable, hydrophobic membrane is disposed at the enzyme electrode in place of the known hydrophilic separating membrane between the reaction layer of the sandwich membrane and blood sample of the individual, which is perforated in a defined extent. Die Perforation der Membran wird zur Verhinderung des Abspülens des unter der hydrophoben Membran befindlichen trägerfixierten Enzyms mit einer zusätzlichen hydrophilen Membran verschlossen. The perforation of the membrane is sealed to prevent the rinsing of the hydrophobic membrane located below the carrier-fixed enzyme having an additional hydrophilic membrane. Mit der Größe der Perforation kann die Steilheit der Eichkurve der Enzymelektrode geändert werden. With the size of the perforation, the steepness of the calibration curve of the enzyme electrode can be changed. Zur Variation der Grenzen des linearen Meßbereiches des Sensorstroms in Abhängigkeit von der Blutglukosekonzentration wird erfindungsgemäß die Perforation in der hydrophoben Membran der Sandwich-Membran exzentrisch über dem Elektrodensystem des Enzymsensors angeordnet. In order to vary the limits of the linear measuring range of the sensor current as a function of the blood glucose concentration, the perforation is placed into the hydrophobic membrane of the sandwich diaphragm eccentrically over the electrode system of the enzyme sensor according to the invention.
DD23981582A 1982-05-13 1982-05-13 Enzyme electrode for glucose measurement DD227029A3 (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
DD23981582A DD227029A3 (en) 1982-05-13 1982-05-13 Enzyme electrode for glucose measurement

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
DD23981582A DD227029A3 (en) 1982-05-13 1982-05-13 Enzyme electrode for glucose measurement
BG6049083A BG42633A1 (en) 1982-05-13 1983-04-08 Enzymic electrode for measuring glucose content
CS832656A CS265683A1 (en) 1982-05-13 1983-04-13 Enzymova elektroda k mereni glukozy

Publications (1)

Publication Number Publication Date
DD227029A3 true DD227029A3 (en) 1985-09-04

Family

ID=5538548

Family Applications (1)

Application Number Title Priority Date Filing Date
DD23981582A DD227029A3 (en) 1982-05-13 1982-05-13 Enzyme electrode for glucose measurement

Country Status (3)

Country Link
BG (1) BG42633A1 (en)
CS (1) CS265683A1 (en)
DD (1) DD227029A3 (en)

Cited By (23)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0327018A2 (en) * 1988-02-05 1989-08-09 Nova Biomedical Corporation Enzyme Electrode
DE4104776A1 (en) * 1990-08-31 1992-03-05 Inst Molekularbiologie Ak Sandwich enzyme membrane for enzyme or opto electrodes - consists of of an enzyme layer coated with membrane of inhomogeneous permeability for substrate
US5183550A (en) * 1989-06-30 1993-02-02 Dragerwerk Aktiengesellschaft Measuring cell for electrochemically detecting a gas
US5352348A (en) * 1987-04-09 1994-10-04 Nova Biomedical Corporation Method of using enzyme electrode
EP0778897A1 (en) * 1994-09-01 1997-06-18 Adam Heller Subcutaneous glucose electrode
DE4221848C2 (en) * 1992-07-03 2001-04-12 Eckhard Salzsieder Method and arrangement for the automatic in situ calibration of intracorporeal Glukosemeßeinrichtungen
US6484046B1 (en) 1998-03-04 2002-11-19 Therasense, Inc. Electrochemical analyte sensor
US6576101B1 (en) 1997-02-06 2003-06-10 Therasense, Inc. Small volume in vitro analyte sensor
US6591125B1 (en) 2000-06-27 2003-07-08 Therasense, Inc. Small volume in vitro analyte sensor with diffusible or non-leachable redox mediator
US6592745B1 (en) 1998-10-08 2003-07-15 Therasense, Inc. Method of using a small volume in vitro analyte sensor with diffusible or non-leachable redox mediator
US7861397B2 (en) 1998-03-04 2011-01-04 Abbott Diabetes Care Inc. Method of making an electrochemical sensor
US8175673B2 (en) 1998-04-30 2012-05-08 Abbott Diabetes Care Inc. Analyte monitoring device and methods of use
US8668645B2 (en) 2001-01-02 2014-03-11 Abbott Diabetes Care Inc. Analyte monitoring device and methods of use
US8688188B2 (en) 1998-04-30 2014-04-01 Abbott Diabetes Care Inc. Analyte monitoring device and methods of use
US9625413B2 (en) 2006-03-31 2017-04-18 Abbott Diabetes Care Inc. Analyte monitoring devices and methods therefor
US9668684B2 (en) 2009-02-26 2017-06-06 Abbott Diabetes Care Inc. Self-powered analyte sensor
US9669162B2 (en) 2005-11-04 2017-06-06 Abbott Diabetes Care Inc. Method and system for providing basal profile modification in analyte monitoring and management systems
US9743863B2 (en) 2006-03-31 2017-08-29 Abbott Diabetes Care Inc. Method and system for powering an electronic device
US9801545B2 (en) 2007-03-01 2017-10-31 Abbott Diabetes Care Inc. Method and apparatus for providing rolling data in communication systems
US9962091B2 (en) 2002-12-31 2018-05-08 Abbott Diabetes Care Inc. Continuous glucose monitoring system and methods of use
US9980670B2 (en) 2002-11-05 2018-05-29 Abbott Diabetes Care Inc. Sensor inserter assembly
US10231654B2 (en) 2005-11-01 2019-03-19 Abbott Diabetes Care Inc. Analyte monitoring device and methods of use
DE102017126732A1 (en) * 2017-11-14 2019-05-16 Endress+Hauser Conducta Gmbh+Co. Kg Sensor diaphragm, sensor, a measuring device comprising the sensor and use of the sensor

Families Citing this family (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE10009467A1 (en) * 2000-02-28 2001-09-20 Bcs Bio Und Chemosensoren Gmbh Enzymatic electrochemical measuring device, for determining glucose in urine, comprises a sensor based on a pair of Clark electrodes, where only one contains an enzyme

Cited By (41)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5352348A (en) * 1987-04-09 1994-10-04 Nova Biomedical Corporation Method of using enzyme electrode
EP0327018A3 (en) * 1988-02-05 1990-04-11 Nova Biomedical Corporation Enzyme electrode
EP0327018A2 (en) * 1988-02-05 1989-08-09 Nova Biomedical Corporation Enzyme Electrode
US5183550A (en) * 1989-06-30 1993-02-02 Dragerwerk Aktiengesellschaft Measuring cell for electrochemically detecting a gas
DE4104776A1 (en) * 1990-08-31 1992-03-05 Inst Molekularbiologie Ak Sandwich enzyme membrane for enzyme or opto electrodes - consists of of an enzyme layer coated with membrane of inhomogeneous permeability for substrate
US6514718B2 (en) 1991-03-04 2003-02-04 Therasense, Inc. Subcutaneous glucose electrode
DE4221848C2 (en) * 1992-07-03 2001-04-12 Eckhard Salzsieder Method and arrangement for the automatic in situ calibration of intracorporeal Glukosemeßeinrichtungen
EP0778897A4 (en) * 1994-09-01 2000-03-22 Adam Heller Subcutaneous glucose electrode
EP0778897A1 (en) * 1994-09-01 1997-06-18 Adam Heller Subcutaneous glucose electrode
US8105476B2 (en) 1997-02-06 2012-01-31 Abbott Diabetes Care Inc. Integrated lancing and measurement device
US6576101B1 (en) 1997-02-06 2003-06-10 Therasense, Inc. Small volume in vitro analyte sensor
US8118992B2 (en) 1997-02-06 2012-02-21 Abbott Diabetes Care Inc. Small volume in vitro analyte sensor
US7988845B2 (en) 1997-02-06 2011-08-02 Abbott Diabetes Care Inc. Integrated lancing and measurement device and analyte measuring methods
US6484046B1 (en) 1998-03-04 2002-11-19 Therasense, Inc. Electrochemical analyte sensor
US7861397B2 (en) 1998-03-04 2011-01-04 Abbott Diabetes Care Inc. Method of making an electrochemical sensor
US8391945B2 (en) 1998-04-30 2013-03-05 Abbott Diabetes Care Inc. Analyte monitoring device and methods of use
US8666469B2 (en) 1998-04-30 2014-03-04 Abbott Diabetes Care Inc. Analyte monitoring device and methods of use
US8175673B2 (en) 1998-04-30 2012-05-08 Abbott Diabetes Care Inc. Analyte monitoring device and methods of use
US8226555B2 (en) 1998-04-30 2012-07-24 Abbott Diabetes Care Inc. Analyte monitoring device and methods of use
US8641619B2 (en) 1998-04-30 2014-02-04 Abbott Diabetes Care Inc. Analyte monitoring device and methods of use
US8688188B2 (en) 1998-04-30 2014-04-01 Abbott Diabetes Care Inc. Analyte monitoring device and methods of use
US8372005B2 (en) 1998-04-30 2013-02-12 Abbott Diabetes Care Inc. Analyte monitoring device and methods of use
US8235896B2 (en) 1998-04-30 2012-08-07 Abbott Diabetes Care Inc. Analyte monitoring device and methods of use
US8226815B2 (en) 1998-10-08 2012-07-24 Abbott Diabetes Care Inc. Small volume in vitro sensor and methods of making
US6592745B1 (en) 1998-10-08 2003-07-15 Therasense, Inc. Method of using a small volume in vitro analyte sensor with diffusible or non-leachable redox mediator
US9891185B2 (en) 1998-10-08 2018-02-13 Abbott Diabetes Care Inc. Small volume in vitro analyte sensor
US8701282B2 (en) 1998-10-08 2014-04-22 Abbott Diabetes Care Inc. Method for manufacturing a biosensor
US6591125B1 (en) 2000-06-27 2003-07-08 Therasense, Inc. Small volume in vitro analyte sensor with diffusible or non-leachable redox mediator
US9662057B2 (en) 2000-06-27 2017-05-30 Abbott Diabetes Care Inc. Integrated sample acquisition and analyte measurement method
US8668645B2 (en) 2001-01-02 2014-03-11 Abbott Diabetes Care Inc. Analyte monitoring device and methods of use
US9610034B2 (en) 2001-01-02 2017-04-04 Abbott Diabetes Care Inc. Analyte monitoring device and methods of use
US9980670B2 (en) 2002-11-05 2018-05-29 Abbott Diabetes Care Inc. Sensor inserter assembly
US9962091B2 (en) 2002-12-31 2018-05-08 Abbott Diabetes Care Inc. Continuous glucose monitoring system and methods of use
US10231654B2 (en) 2005-11-01 2019-03-19 Abbott Diabetes Care Inc. Analyte monitoring device and methods of use
US10201301B2 (en) 2005-11-01 2019-02-12 Abbott Diabetes Care Inc. Analyte monitoring device and methods of use
US9669162B2 (en) 2005-11-04 2017-06-06 Abbott Diabetes Care Inc. Method and system for providing basal profile modification in analyte monitoring and management systems
US9625413B2 (en) 2006-03-31 2017-04-18 Abbott Diabetes Care Inc. Analyte monitoring devices and methods therefor
US9743863B2 (en) 2006-03-31 2017-08-29 Abbott Diabetes Care Inc. Method and system for powering an electronic device
US9801545B2 (en) 2007-03-01 2017-10-31 Abbott Diabetes Care Inc. Method and apparatus for providing rolling data in communication systems
US9668684B2 (en) 2009-02-26 2017-06-06 Abbott Diabetes Care Inc. Self-powered analyte sensor
DE102017126732A1 (en) * 2017-11-14 2019-05-16 Endress+Hauser Conducta Gmbh+Co. Kg Sensor diaphragm, sensor, a measuring device comprising the sensor and use of the sensor

Also Published As

Publication number Publication date
BG42633A1 (en) 1988-01-15
CS265683A1 (en) 1985-06-13

Similar Documents

Publication Publication Date Title
DE3019870C2 (en)
EP1593958B1 (en) Disposable Test Strips with integrated reagent/blood seperation layer
US4404066A (en) Method for quantitatively determining a particular substrate catalyzed by a multisubstrate enzyme
US6749740B2 (en) Small volume in vitro analyte sensor and methods
DE69629051T2 (en) Trouble-free biosensor
Drumond et al. Structural basis for reduced glomerular filtration capacity in nephrotic humans.
DE69535334T2 (en) A process for the preparation of amperometric electrodes
US4197852A (en) Catheter electrode for electrochemical analysis
AT402452B (en) Planar sensor for determining a chemical parameter of a sample
AT411627B (en) Apparatus for checking the positioning and the absence of bubbles in a medical microsample in a flow cell
US7807031B2 (en) Disposable test strips with integrated reagent/blood separation layer
EP0025110A2 (en) Electrochemical measuring apparatus provided with an enzyme electrode
DE2833741C2 (en)
DE60132955T2 (en) biosensor
US4897162A (en) Pulse voltammetry
DE69333400T2 (en) A method for improving the performance of electrochemical sensors
DE69734673T2 (en) Device for non-invasive determination of glucose in body fluids
DE60119133T2 (en) biosensor
EP2322645A1 (en) Mass transport limited in vivo analyte sensor
DE2620693C2 (en)
EP1181539B1 (en) Disposable sub-microliter volume sensor
DE60306711T2 (en) Method and device for collection of physiological samples
DE69917372T2 (en) Device for quantification of substrates
DE69920006T2 (en) Method and apparatus for electrochemical measurements using statistical methods
DE60018541T2 (en) biosensor

Legal Events

Date Code Title Description
RPI Change in the person, name or address of the patentee (searches according to art. 11 and 12 extension act)
RPV Change in the person, the name or the address of the representative (searches according to art. 11 and 12 extension act)
UW Conversion of economic patent into exclusive patent
ENJ Ceased due to non-payment of renewal fee