DD223057A1 - METHOD FOR THE DEPOSITION OF PROTEINS FROM PROTEIN-BASED WASTEWATERS - Google Patents

Abstract

The invention relates to a method for the separation of protein from the effluents of the starch industry (potato fruit juice) and the slaughtering and processing plants. The object of the invention is to develop a cost-effective method for recovering the crude protein from these effluents. The invention has for its object to develop a method for flocculation and separation of a possible large proportion of high-quality nutrients of the above wastewater. According to the invention, the object is achieved by lowering the p H value of the wastewater, by coagulating the protein by using cost-effective flocculating agents, by separating the physiologically valuable substances using raw-fiber-rich auxiliaries, and by separating them Solid and supernatant with known mechanical devices takes place.

Description

Dry substance in% 6.5 18.4 Crude protein in g / kg of original substance 30.5 91.9 g / kg dry substance ¹ 469 499

In addition to belt presses, decanters and separators can also be used for separation. Even without the addition of auxiliaries, the separation of the protein constituents flocculated with sulfite liquors or carnauba liquors by means of flotation plants is possible. The technical and economic effects of the invention are as follows:

- The industrial recovery of protein with a high biological value without additional agricultural land by using a waste product and using waste products as auxiliaries

- Satisfying the increasing demand for protein both in agriculture and in human nutrition

- exploitation of industrial by-products

- the potential use of existing technologies to obtain the product.

embodiments

1st example. ",."

100 ml of potato fruit juice (Kfw) are acidified with hydrochloric acid or sulfuric acid to pH 4.0 and treated with spruce sulphite liquor (1% by volume). After a 10-minute service life, centrifuge with the laboratory centrifuge (5min, 3000rpm). For analysis, the Kfw used, the supernatant and the separated solid go. - /

Kfw solid supernatant

5.6

14.5 259

2nd example \ ^

1601 Kfw are acidified with hydrochloric acid to a pH of 4.1 and mixed with 1% by volume of spruce sulphite liquor and stirred briefly. After a service life of 120min, it is separated with a separator. In parallel, 200 ml of the mixture are removed and centrifuged (5 min, 3 000 U / min). For analysis come the Kfw, the solid and the liquid phase from the separator, as well as the supernatant from centrifuging. ·.

, Kfw solid fl. Phase supernatant

Dry substance in% · 5.9 '; , 18,0. 5.9 4.6

Crude protein in, ν '. '

g / kg original substance 31.5 85.0 23.8 15.2

g / kg dry substance, 534 474 403 330

3rd example

2001 Kfw are acidified with hydrochloric acid to pH 4.0. Then 0.5% by volume of spruce sulfite liquor is added with stirring. After about 20 minutes, the addition of 6 kg pulp, which has a dry solids content of 8.6% after a previous hot steam treatment. After a further 60 minutes, the separation of the mixture takes place via a separator. For analysis, the Kfw, the solid, the liquid from the separator and the supernatant solution obtained after centrifuging the starting mixture with a laboratory centrifuge (5miri, 3000U / min).

Kfw solid fl. Phase supernatant

Dry substance in%. 6.4 '15.1 5.4 5.0

Crude protein in.

g / kg of original substance 37.2 50.9 46.5 44.2

g / kg dry substance '581 -509' 465 442 '. ...

4. Example!

11001 Kfw are acidified with hydrochloric acid to a pH of 4.6. Thereafter, 1.0% by volume Fichtensulfitablauge is added with stirring. After approx. 20 minutes the addition of 93 kg of straw flour takes place. After another 30 minutes, the mixture is squeezed through belt presses. The Kfw and straw meal used and the resulting solids are analyzed.

Kfw straw flour solid

Dry substance in%. 6.0 '90.4 32.0',

Crude protein in - '

g / kg of original substance 32.6 34.4 31.0

g / kg dry matter - 548. 38.0 ^: 97.0

5th example -

10 ml of slaughterhouse waste water are acidified with hydrochloric acid to pH 4.5 and treated with spruce sulphite waste liquor (1% by volume). After 1 min. Life the precipitate is separated with the laboratory centrifuge (5min, 3000rpm). Analyzed: ^v were the sewage, the supernatant and the solid. - '

, Sewage solid supernatant

Dry matter in% ' ί 9.8 25.4 4.3

Crude protein in. ·.

g / kg of original substance 61, Ö 192 14.0 '

g / kg dry substance 622 756 326

Claims (5)

-1 - zsö a ^ o o Invention claims: 1.0. A process for the deposition of protein from protein-containing waste water, characterized in that the waste water is kept at the temperature of the of the present the process temperature (5-3O ⁰ C), in that acidification of the protein containing waste water to a pH of 3, 0 to 7.5, preferably 4.0 to 5.0 that is carried out for the coagulation of the protein sulfite liquors and / or magnesium chloride and / or aluminum sulfate and / or ferric chloride and / or calcium chloride and / or Kamallitlauge and / or Fichtensulfitablauge in an amount of 0.01 to 5 wt .-% as a flocculant added that then depending on the separation technology raw fiber-rich materials such. B. pulp and / or cellulose and / or straw meal, and / or starch in a Menge.von 0.5 to 50 wt .-% and that the separation of the protein with known mechanical devices such as belt presses, decanters, separators and flotation plants. , 1.1. Process according to item 1.O., characterized in that the raw fiber-rich materials pulp and cellulose and starch at a temperature of 50 to 100 ⁰ C, in particular at 65 to 80 ° C thermally pretreated. 1.2. Procedure according to point 1.0. and 1.1., characterized in that the thermal pretreatment of the pulp, cellulose and starch takes place directly on the belt presses by heatable rollers. ''. 1.3. Procedure according to point 1.0. to 1.2., Characterized in that when using flotation systems, an addition of raw fiber-rich materials and starch is not required. _ ,. 1.4. Procedure according to point 1.0. to 1.3, characterized in that the solid obtained is used as feed. ι ... Field of application of the invention The invention relates to a method for the separation of proteins from potato fruit juice (starch industry) and from waste water from the slaughtering and processing plants. Characteristic of the known technical solutions The potato fruit juice is a waste of the starch industry and is produced in an amount that corresponds approximately to the amount of potato processing. With a TS content of 5-6%, large amounts of high-quality nutrients are recovered each year either from non-high-cost or high-cost processes. Approximately 40-60% of the TS is accounted for by the crude protein. The production of the protein from potato fruit water has been intensively pursued for some time. In addition to the long-standing practice of heat coagulation, ultrafiltration and reverse osmosis have recently been used in practice. While heat coagulation requires a lot of energy, ultrafiltration and reverse osmosis cause high technological costs. All previous methods are therefore not suitable to produce a cost-effective protein-containing feed. Protein-containing wastewater accumulates in the slaughterhouse and processing plant, especially when large quantities of blood and the cooking water from the BrühwursthersteHung get into the wastewater. The composition of the waste water is quite different and depends on the technology and the range to be produced by the relevant company. In addition to proteins, especially fats are still contained in the wastewater. The treatment of slaughterhouse waste water takes place after a fat separation in the sedimentation basin. In this basin, the settling of the coarse ingredients and partly a biological treatment of the wastewater takes place. In modern slaughterhouses flotation plants are used for cleaning. An improved effect is achieved if flocculants are previously added. At present, aluminum sulfate and iron (II) chloride are used. A use of the deposited product as animal feed is currently being carried out on a trial basis and should be completely prohibited in the future when using aluminum salts. ' Object of the invention The aim of the invention is to carry out the extraction of the crude protein from potato fruit juice and waste water from the slaughter and processing plants as inexpensively as possible. Explanation of the essence of the invention The invention has for its object to develop a method for flocculation and separation of the largest possible proportion of high-quality nutrients of potato fruit juice and waste water from slaughter and processing plants (hereinafter referred to uniformly as wastewater). According to the invention the object is achieved in that the pH of the waste water is lowered, that coagulation of the protein is carried out by the use of inexpensive flocculants and that a separation of the physiologically most valuable substance is partially enabled by using crude fiber-rich tools. The flocculants used are ferric chloride and aluminum sulfate, which are already known from the treatment of waste water and drinking water. But it is also possible to use calcium chloride or magnesium chloride. However, the use of sulfite waste liquor as a product of paper and pulp mills or the use of kaallite liquor as a by-product of table salt production is particularly favorable. The use of spruce sulphite waste liquor is ultimately possible and particularly effective. To carry out the process, the pH of the wastewater is lowered to 3.0... 7.5, and the entire protein precipitable with trichloroacetic acid is precipitated by adding, for example, spruce sulphite liquor. The result is a feinfiockiger precipitate, which can be deposited according to the prior art only by means of centrifuges or high-performance nozzle separators. According to the invention to improve the separation of the precipitate after acidification and the addition of the flocculant raw fiber-rich materials such. As pulp, cellulose and straw, or starch added. By partial attachment of the coagulated protein flakes to the excipient, improved separation of the protein is achieved. By a thermal treatment in particular of the pulp or the starch, up to 60 ... 100 ° C, the properties required in the context of the invention further improved. The added amount of raw fiber-rich material depends on the available separation technology or on the nature of the product to be produced and is between O ... 5OMa .-%. If large quantities of the raw fiber-rich material are added, the belt presses used to press off the pulp in the starch industry can already be used. This technology produces a high fiber rich protein enriched nutrient that can be used as a valuable feed in animal nutrition.