DD210352A1 - SENSITIVE MICROELECTRODE FOR DETERMINING NITRATION ACTIVITIES - Google Patents

Abstract

The invention relates to the above-mentioned. An electrode consisting of a glass capillary tip filled with a liquid-impermeable nitrate-sensitive membrane and an internal reference insert with Ag / AgCl reference element. Such an electrode must withstand the pressure of piercing the cell to be examined. Therefore, the membrane should adhere reliably to the glass surface. According to the invention, this is achieved by siliconized the glass capillary and the membrane consists of tetraalkyl or triarylalkylphosphonium nitrate, which in the ratio 1:20 in o / p-nitrophenyl n-octyl ether with a paint (ratio of ether: paint = 1: 1), as it is normally used as a parquet sealant, consisting essentially of components based on phthalic anhydride and a Aminoplastlackharzes, solved and cured. The microelectrode is particularly suitable for the investigation of dead and living cells.

Description

! Eitel.der invention '

Sensitive microelectrode for the determination of hitration activities

Field of application of the invention

The invention relates to a sensitive microelectrode for purifying nitrate activities, for example, plants and / or in animal cells, consisting of a glass capillary, the tip of which. a liquid-impermeable nitrate-sensitive membrane is filled and an internal reference insert with Ag / AgG1 reference element.

Characteristic of the known - technical solutions

It is known to use various sensitive electrolyte electrodes for intracellular measurements. Thus Hinke in M, Lavallee, 0 · Ρ · Schanne, H * C * Hebert. (Eds) glass microelectrodes ,. Wiley; Sons Inc. Hew York, London, Sydney, Toronto 1969, pH-uhd ^; liatrdSm ^ ^{microelectrodes;} bescnfie'ben, Dereh "Slektro (iehspitze ⁿ of pH or" Ufa-a Sensitive Glass existing

2aSER1982 * O37867

- 2 -, .. P 906

For potassium-, calcium- and chloride-sensitive microelectrode! glass capillary tips are filled with suitable ion exchangers as sensor material (M, Oehme, W., Somin, Anal * CMm * Acta 86 21 (1976) and I, Oehme Diasertation BTH Zurich.1977 *

Useful microelectrodes for the determination of nitrate activities in cells have hitherto not been possible because the membrane compositions known from the macroelectrodes with tetraalkyl- or triarylalkylphosphonium nitrate as active component and PVC as support material (DD-PS 127 372) are not suitable for this purpose the poor adhesion of the membrane material to the glass surface in the capillary tip, which leads to puncture inability.

Object of the invention

The aim of the invention is to provide a nitrate-sensitive microelectrode for measuring ITitrationenaktivität in plant and animal cells. develop * The Blektrodehspitzen diameter should be 1 to 5 / Um *

Explanation of the essence of the invention

The invention is therefore based on the Aufgäbe, a sensitive microelectrode for determining ": Hitrationenaktivitäten

'· ·

zn develop whose capillary tip and the membrane should be such that a reliable adhesion of the membrane is ensured on the glass surface in the capillary tip.

Invention, this is achieved according to _t by the glass capillary is siliconized and the membrane consists of tetra or Triary1-alkyIphosphoniumnitrat is, the ratio of 1: 20 in o / p-Hitrophenyl-n-octylather (with a lacquer ratio ether: Paint = 1: 1), which is normally used as parquet

- 3 - P 906

SiegeI ^- UiIgSlack is used, consisting essentially of components based on phthalic anhydride and a Aminoplastlackharzes, dissolved and cured.

The curing time after pulling the glass capillary is several days. Then the reference solution can be filled in and the electrode is ready to measure.

In this way microelectrodes with a tip diameter of 1 to 5 / Tius and a membrane layer thickness of approx. 200 can be realized *

The so made. Membrane adheres tightly to the glass surface and withstands the pressure of piercing the microelectrode into the plant cell. In addition, the novel carrier material does not increase the electrical resistance. With the microelectrode, titration activities from 10 to TO ·· M can be measured.

Ausfünrungsbeispiel

By way of example, the invention will be explained in more detail. In one: .- 5 ^; cm; Lengths: siliconized glass capillary with ^: a tip diameter of 2 / around 200 to thick. , nitrate-sensitive membrane. This consists of Tributyloctadecylphosphoniumnitrat dissolved 1:20 in o / p-litrophenyl-n-octyl ether and varnish with Parkettversiege as carrier material 1.: Ί mixed. The mentioned paint consists of the following components:

- castor oil, a glycerine substitute of the purest ethylene

'Lj

glycol and phthalic anhydride dissolved in butyl acetate,

- Tall oil fatty acid, pentaerythritol, a glycerin substitute

, i>

from the purest ethylene glycol and phthalic anhydride dissolved in xylGl / butanol.

Acid-hardening wood varnish of medium viscosity in the form of an aminoplastic varnish resin, consisting of urea and formaldehyde, etherified with n-butanol and present in 5% strength solution of n-butanol.

- 4- P 906

It should be noted that the acid-curing Hoizlack added as the last component.

The glass capillary is after curing of the nitrate-sensitive membrane with 0.1 M KMO ₃ ; 0.1 M KCl mixed solution filled bubble-free and completed by means of Ag / AgCl-discharge electrode to the microelectrode.

Claims (1)

  - 5 - P 906 Ξrfindungaanspruch Sensitive microelectrode for determining uritration activities, consisting of a glass capillary, the tip of which is filled with a liquid-impermeable nitrate-sensitive membrane, and an internal reference insert with Ag / AgCl reference element, characterized in that the glass capillary is siliconized and the membrane of tetraalkyl or triarylalkylphosphonium nitrate ^: consisting in the ratio of 1: 20 in o / p-iTitrophenyl-n-oetylather with a varnish (ratio ether: varnish = 1: 1), as it is normally used as a parquet seal varnish, consisting essentially of components on which Base of phthalic anhydride and an aminoplast resin 'V dissolved and cured