DD160357A3 - METHOD FOR PRODUCING LYSINE-ENRICHED FODDER WHITE CONCENTRATE - Google Patents

Abstract

The invention relates to a process for the production of lysine-enriched feed concentrate with a high nutrient value by cultivating microorganisms on hydrocarbons. The aim of the invention is to inexpensively produce lysine-enriched feed concentrate without the need for additional active ingredients in lysine production, excluding the formation of difficult-to-apply products. An unsterile microbiological process for the production of feed wheat based on n-alkane petroleum products is combined with a microbial sterile process to obtain lysine based on liquid n-paraffins. The lysine-containing microorganism suspension is combined with the yeast suspension and subjected to a common purification process to remove unwanted hydrocarbons.

Description

Title of the invention

Process for the preparation of lysine enriched ^E ] utternxweißkonzentrat

C 12 1 Application Area of the Invention

The invention relates to a process for the production of lysine-enriched feed protein concentrate with a high nutritional value by cultivating microorganisms on hydrocarbons.

Characteristics of the "known technical solution en

L - lysine plays an important role in the metabolism of animal life.

In particular, in the physiologically correct diet of trimmings, primarily those for meat production, the qualitatively and quantitatively adequate G-abe of lysine with the feed is an indispensable requirement, In the commonly available conventional feed from agricultural production lysine is not known to be sufficiently contained , Many efforts are therefore made to enrich lysine in these feeds in the form of additives.

Predominantly, lysine is obtained by microbial route. Numerous methods are known for this, which can be divided into two main groups by the carbon source:

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1, Microbial processes based on carbohydrates or other water-soluble substrates.

2. Microbial processes based on liquid n-alkanes (Cj ρ - C30) ^a ^ ^s carbon source.

The disadvantage is:

1. The sources of carbon are expensive, especially when using carbohydrates, as sugar is generally used. IEU ß.

As a result, the end product lysine as a feed additive reaches prices that are above the prices of human foods.

In addition, the growth of lysine-producing microorganisms in the sterile process requires biological agents that are costly.

2. The workup of Lysinkonzentraten to Reinlysin leads to considerable difficulties, since the use of carbohydrates as carbon source usually difficult to apply Bndprodukte arise.

According to SU-PS 1959952 a technical solution to circumvent these problems is proposed.

Although raw material costs are relatively low when using n-alkanes as carbon source, the use of active substance concentrates is indispensable for microbial lysine production.

To make matters worse, the exhausted η - alkanes must be removed from the final product.

These difficulties are the reason why no industrial process of n-alkane-based lysing is known so far.

Object of the invention

The object of the invention is the cost-effective production of lysine-enriched IPuttie white concentrate with no additional requirement of active ingredients in lysine production, excluding the formation of products which are difficult to apply.

Presentation of the essence of the invention

The invention has for its object to develop a method for producing a lysine-enriched Puttereiweißkonzentrates for animal nutrition that works without additional addition of drugs and increases the performance of microorganisms and promotes the task. In accordance with the present invention, a sterile microbial process for η-paraffin lysing recovery is coupled with an unsterile yeast-growing process to n-alkane petroleum distillate.

The unsterile process for breeding yeasts on η-alkane-containing petroleum products has an egg series

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biological advantages ·

Tm unprotected open biological Syster. A number of bacteria find an economic niche in growing on metabolic and lyse products of yeast cells without being able to utilize n-alkanes themselves. These bacteria eliminate the substances harmful to yeast growth and themselves form a series of accessory agents necessary for the growth of yeasts on n-alkanes. The resulting symbiosis results in an optimal fast yeast growth and thus allows a technically - economically favorable yeast production.

The culture fluid obtained in this method after separation of the yeast cells contains growth-promoting Paktoren in physiologically optimal composition, since they stem from a steady state of cells, which are in direct interaction with each other and are opposed substrate recyclers derived.

In the process of yeast cultivation, the culture liquid is used repeatedly by recycling. Complete use according to the state of the art was in most cases not possible because, if the circulation is too frequent, undesirable accumulations of other substances, e.g. inorganic ash formers, kommeη can.

Part of this valuable culture fluid, which has been absorbed into active substances, must therefore be destroyed as waste with the wastewater. This valuable culture fluid is now fully utilized by means of the technical solution according to the invention.

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The lysine-producing bacteria growing on n-alkanes, such as Acinetobacter species, reach their greatest activity in a culture medium taken from an unsterilised feed yeast process of n-paraffinic petroleum products. Lysine concentrations of up to 40 g / l were reached in this culture medium after 36 hours. This culture medium contains about 500 mg / 1 of unreacted hydrocarbons, some of which have been physically dissolved, some are collodially dissolved as miscalls and are inaccessible to mechanical separation. In the work-up of this suspension, the hydrocarbons are contained in the concentrate in an undesirably high amount.

The control of the separation or purification can be achieved in the coupling of the process with a Erdöldestillathefepprozesses in a technically favorable manner as follows:

A particular feature of the processes for obtaining petroleum distillate putty is that the separated yeast cells must be subjected to an intensive cleaning process, usually after a previous drying, to remove the residual hydrocarbons.

This peculiarity can be utilized for the purification of the lysine-microorganism suspension obtained on n-alkanes by coupling both methods. For example, if Acinetobacter is grown under certain biological conditions in the sterile process for lysine production, the final n-alkane microorganism lysine suspension, optionally after a previous thermal concentration, the yeast suspension before the drying process or after the drying process added and a common cleaning

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be subjected to the removal of the residual hydrocarbons.

The coupling of a n-paraffin-based sterile microbial lysing process to an n-alkane petroleum distillate non-sterile process of yeast production provides significant benefits to the biological performance of the lysine production process and to the workup of the n-alkane-containing and lysine-containing culture suspension.

In the final consequence, a high-purity yeast biomass is obtained, which has been enriched with lysine as needed, and is available as lysine-enriched feed protein concentrate of animal nutrition. The culture fluid obtained from the non-sterile process of microorganism growth on petroleum products is also very effective for lysine-producing η-alkane recyclers in terms of growth-promoting agents.

By using the culture liquid in the sterile process and their processing to purified lysine-enriched feed protein concentrate produced in the unsterile Puttereiweißprozeß on Srdöldestillatbasis no waste culture solution.

Overall, the advantages achieved by the industrial introduction of n-alkane use for lysine production much easier * The invention will be explained in more detail by the following example:

example

In a 500 1 stirred fermentor Lodderomyces elongisporus continuously under known conditions

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the base of n-alkane petroleum distillate bred in the non-sterile process. The technological conditions are as follows:

of the fermenter 200 kg

Yerweilzeit 4 H specific ventilation 80 specific benefit claim by the stirrer 3 kW / t temperature 33 ⁰ C 306 K PH value - 4 2 petroleum distillate 15 io n paraffin content in petroleum distillate 17 io

UH -, - water, phosphoric acid and a solution consisting of ZCl, MgSO.sub.2, ZnSO.sub.2, PeSO.sub.4, MnSO.sub.4, are added as the inorganic substances of Fe in a physiologically balanced amount.

The microorganism suspension is continuously withdrawn from the fermentor and subjected to decantation The aqueous, yeast-free culture fluid separated by means of decantation is for the most part returned to the per-mentor.

A partial flow is used to cultivate lysine-producing acinetobacteria as described below.

The yeast-enriched phase from the decantation is heated to 343 K and first the petroleum distillate with the addition of 0.5 g / kg of a surfactant of ethylene oxide and propylene oxide separated in a Tellerseparator. Subsequently, this suspension is concentrated in a jet separator by separation of further aqueous culture fluid to 21 % HTS.

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The culture fluid is also returned to the fermenter.

As mentioned above, 3-5 l / h of culture fluid are continuously withdrawn from the decantation vessel and sterilized by heating to 368 K for 30 minutes.

This culture fluid is used as a basal broth for the growth of Acinetobacter species on 6 $ purified n-alkanes of chain length Cmc ~ "0-4 in a second discontinuous sterile fermentor.

including the following

The basic nutrient solution em; üäJ 3.23 Lt un Amino acids: 1.01 lysine 0.8 mg / 1 histidine 4.82 mg / 1 arginine 2.06 mg / 1 asparagine 11, 48 mg / 1 threonine 380.3 mg / 1 serine 0.9 mg / 1 glutamine 42.5 mg / 1 proline 190.4 mg / 1 glycine 39.73 mg / 1 alanine 17.78 mg / 1 cystine 4.8 mg / 1 YaI in 6.90 mg / 1 methionine 13.19 mg / 1 isoleucine 17,20 mg / 1 leucine 8.81 mg / 1 tyrosine mg / 1 phenylalanine mg / 1

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The cultivation takes place under known conditions at a temperature of 309 K and a pH of 7.1.

After completion of the lysine phase, 34 h after inoculation, a lysine concentration of 31 g / l und.eine biomass concentration of 28 g / l. This resulting suspension was concentrated in vacuo so that the concentration of lysine 93 g / l ·

Thereafter, the suspension is mixed in a ratio of 1: 5 (1 part lysine suspension, 5 parts yeast suspension) with the previously obtained yeast suspension and processed in e-inem spray drier to dry powder with a water content of 9.2 fo · This dry powder still contains 5.1 % Hydrocarbons from both processes.

In a 5-stage extraction, this powder is purified in a ratio of 1: 1 vol, - $> with a mixture of ethanol and gasoline.

The dry yeast has 21 g of lysine per 10Og of protein after purification.

On the other hand, in normal yeast-based dietary protein, only about 7 g of lysine are contained per 100 g of protein.

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Claims (1)

228499 Brfindungsansprach Process for the preparation of lysine-enriched feed protein concentrate, characterized in that an unsterile microbiological process for the production of. Puttereiweiß based on n-alkane petroleum products is combined with a microbial sterile process for the recovery of lysine on the basis of liquid η-paraffins, wherein the l, ysinhaltige microorganism suspension combined with the yeast suspension and subjected to a joint Besigungsproseß to remove unwanted hydrocarbons.