CZ305177B6 - Diluent for short time preserved insemination doses of boars - Google Patents

Abstract

The present invention relates to a diluent for short time preserved insemination doses of boars, which diluent is characterized in that 1 liter of the aqueous diluent contains 30 to 50 g of glucose, 3 to 10 g of sodium citrate, 0.5 to 2 g of ethylenediaminetetraacetic acid (EDTA), 0.5 to 2 g of sodium carbonate, 0.5 to 2 g of potassium chloride and 2 to 3 g of trioxybenzoic acid.

Description

Technical field

The invention relates to a diluent for short-term preserved insemination batches of boars that do not contain antibiotics.

BACKGROUND OF THE INVENTION

The level of reproduction results, among other things, is determined by the quality and health of the insemination batch. Presently, short-term preservation of semen of breeding boars intended for artificial insemination is provided by preservative diluents with antibiotics. Antibiotic treatment contributes significantly to the protection of sperm from contamination by undesirable microflora, which causes decreased sperm survival and fertility. Microorganisms contaminating boar sperm are one of the important factors that negatively affect the biological quality of sperm. The biochemical activity of microorganisms leads to a decrease in the energy sources of seminal plasma and produces toxic metabolites for sex cells. The insemination doses are stored at 16 to 18 ° C, but microorganisms also multiply at this temperature, so effective protection is needed to dampen this development of undesirable microflora in order to promote longer sperm survival. By using antibiotics it is possible to specifically target and influence the microflora in relation to the reproductive apparatus from both the boar and the sow. Strong microbial contamination of the inseminated semen prevents germs nidation, possibly by clogging the infection causing unwanted death of embryos due to inflammation of the uterine mucosa.

Antibiotics, unfortunately, generally do not only have advantages, but also have theirs against it. The danger of antibiotics lies in the formation of their toxic residues, which can enter the body in various ways and are dangerous due to their toxic load, especially for the human organism, at least very unpleasant.

Another risk of excessive use of antibiotics is resistance. In fact, it is the ability of a microorganism, in this case mainly bacteria, to withstand the action of antibiotic drugs. Gradually, there is a change in the gene pool of microorganisms, which begin to get used to these substances and thus do not respond to them. The spread of antibiotic resistance in the veterinary sphere is closely linked to the state of resistance in the human area. The effectiveness of antibiotics is therefore seriously compromised and represents a global threat to the health of the population. This encourages the search for new bioactive compounds with antimicrobial effects to reduce antibiotics.

SUMMARY OF THE INVENTION

The above-mentioned deficiencies are eliminated by a diluent for short-term preserved canned insemination batches according to the invention, characterized in that 1 liter of aqueous diluent contains 30 to 50 g of glucose, 3 to 10 g of sodium citrate, 0.5 to 2 g of ethylenediaminetetraacetic acid (EDTA). 0.5 to 2 g of sodium carbonate, 0.5 to 2 g of potassium chloride and 2 to 3 g of gallic acid.

The diluent according to the invention is further characterized in that 1 liter of aqueous diluent contains 37 g glucose, 6 g sodium citrate, 1.25 g ethylenediaminetetraacetic acid (EDTA), 1.25 g sodium carbonate, 0.75 g potassium chloride and 2.4 g. g of gallic acid.

The diluent according to the invention serves in particular for the short-term preservation and dilution of semen immediately before the insemination being prepared and removes the mentioned negative impacts of the use of antibiotics by replacing them with gallic acid from natural sources.

-1 CZ 305177 B6

Gallic acid is an organic acid that is found in pimples, nuts, oak bark, tea leaves and other plants where it is either loose or bound to tannins from which it is obtained by acid hydrolysis. This acid has antimicrobial effects and acts as an antioxidant. It inhibits the growth of bacterial strains occurring in boar ejaculate. Gallic acid in preservatives does not present any known health or hygiene risks.

The current strongly increasing resistance of microorganisms requires vigilance in antibiotic therapy, and in particular the elimination of antibiotics from areas where they can be replaced by other substances, potentially reducing the risk of resistance. The antimicrobial effects of some natural substances are being used as a substitute for previously widely used antibiotics. Natural substances are preferred for their minimal toxicity, minimal side effects, good availability and better degradability compared to antibiotics.

Preventing the transmission of pathogenic germs from the boar to the sow by means of an insemination dose has a beneficial effect on reproductive performance, in particular on pregnancy and fertility. The recommended usability of the insemination dose is 1 to 2 days, allowing the optimal time of insemination to be respected. The survivability of the spermatozoa treated with the diluent according to the invention achieved comparable results to the control samples.

The potency of the gallic acid diluent of the invention achieved sperm survival in a short-term preservation test of 89% of the sperm control treated with antibiotic preservatives. The efficacy of the short-term preservative diluent with gallic acid in the experiments 24 hours after dilution was 105% of the control. The most common is the use of insemination doses within 1 to 2 days, due to the timing of the appropriate timing of the insemination, when usually one insemination and one reinsemination (repeated insemination) is required per sow. In comparative experiments, sperm survival achieved results without significant differences with control sperm samples treated with antibiotic diluent.

The diluent according to the invention was successfully tested by the inventor in practice in stables in Kostelec nad Orlicí by the applicant, which is the Research Institute of Animal Production, vv.i., Prague, CZ. The following examples illustrate, but do not limit, the diluent of the present invention.

DETAILED DESCRIPTION OF THE INVENTION

Example 1

37 g of glucose, 6 g of sodium citrate, 1.25 g of ethylenediaminetetraacetic acid (EDTA), 1.25 g of sodium carbonate, 0.75 g of potassium chloride and 2.4 g of gallic acid were weighed into a suitable container and the powder mixture was added. up to 1 liter of pyrogen-free distilled water at 38 ° C. After proper dissolution of all components, the diluent of the invention was prepared for short-term treatment of boar semen.

It is advantageous for transport, when a weighed bulk mixture of all said components is prepared in advance, and only prior to the planned insemination, this mixture is dissolved in pyrogen-free distilled water at 38 ° C to produce 1 liter of the desired non-antibiotic diluent according to the invention.

The resulting 1 liter of diluent according to the invention is sufficient for 15 to 20 insemination doses of boar semen, depending on the use of varying degrees of dilution.

-2GB 305177 B6

Industrial applicability

The novel diluent according to the invention does not contain antibiotics and is therefore much more suitable for short-term treatment and preservation of semen for insemination.

PATENT CLAIMS

Claims (2)

A diluent for short-term preserved insemination batches of boars, characterized in that 1 liter of aqueous diluent contains 30 to 50 g of glucose, 3 to 10 g of sodium citrate, 0.5 to 2 g of ethylenediaminetetraacetic acid, 0.5 to 2 g of sodium carbonate 0.5 to 2 g of potassium chloride and 2 to 3 g of gallic acid. 2. The diluent of claim 1 wherein 1 liter of aqueous diluent comprises 37 g glucose, 6 g sodium citrate, 1.25 g ethylenediaminetetraacetic acid, 1.25 g sodium carbonate, 0.75 g potassium chloride, and 2.4 g. g of gallic acid.