CZ30044U1 - Stimulant - Google Patents

Description

Technical field

The technical solution relates to a composition whose injection into a rock environment, contaminated water or chemically contaminated waste can achieve biodegradation of water-soluble substances such as benzene, toluene, xylene or ethylbenzene. The composition combines substrate and solvent properties.

BACKGROUND OF THE INVENTION

In the current practice of technical microbiology, within the framework of the stimulation of natural processes, in particular concepts that enhance the direct metabolism of the original microflora of the treated locality are applied. These are applications of nitrogen, phosphorus and potassium sources. However, the limiting factors of the limited biodegradability of poorly water-miscible organic contaminants are, in most cases, based on the lack of bioaccessibility of contaminants for the cell degrading the microorganism, although the physiological state and metabolic capacity of the microflora (bioavailability) biologically transformed into less harmful or non-toxic substances.

Direct support of metabolism by the supply of some substrates can develop the so-called cometabolic transformation of contaminants, where, due to overproduction of certain enzymes that are sufficiently non-specific and can catalyze the biodegradation of organic contaminants with certain structural similarities. Direct support of metabolism by supplying macroelements in the form of their salts (ammonium ions, nitrates, phosphates) mostly contributes to a comprehensive supply of microflora with the necessary metabolic resources. The combined effect of direct stimulation in systems with limited miscible organic contaminants in the aquatic environment contributes to the production of self-substances that are amphiphilic due to the structure of their molecule. Tzv. biosurfactants make it easier to bridge the phase boundary, to colonize it, and to facilitate the use of the substrate in the form of organic contaminants. However, there is rarely an overproduction of the biosurfactant under these conditions to satisfactorily accelerate the kinetic and reaction parameters of bioremediation.

The solution used is to apply industrial detergents at diluted concentrations, provided that they exhibit negligible toxicity to the biodegradable microflora and that they do not contradict the regulations for introducing substances into the environment. However, a significant proportion of industrial detergents are persistent and persist in the environment longer than necessary, including possible adverse effects on other organisms.

The essence of the technical solution

The aim of the technical solution is to ensure the stimulation of biodegradation processes against limited miscible organic substances such as benzene, toluene, xylene or ethylbenzene, but at the same time not unnecessarily burden the environment with the presence of another foreign substance. energy (substrate).

This task substantially solves the subject of the technical solution, which is a stimulant for application in sites contaminated, inter alia, with benzene, toluene, xylene and ethylbenzene, whose benefit is a positive influence of degradation activity by the original microorganisms on the contaminated site.

It is based on the simultaneous action of both a solvent (cosolvent) and a substrate (cosubstrate) in a groundwater environment contaminated by poorly miscible organic contaminants such as benzene, toluene, xylene or ethylbenzene.

The essence of the invention is a stimulant which consists of an aqueous mixture of 10 to 20 wt. % ethanol, 5 to 8 wt. % propanol, 0 to 5 wt. % acetic acid and 0 to 5 wt. lactic acid.

-1 CZ 30044 Ul

Examples of technical solutions

The technical solution works with a modified composition of the stimulant, thereby enhancing both properties related to the ability to dissolve nonpolar substances and, secondly, the substrate property, where the stimulant fulfills the function of a metabolically exploitable source.

Example 1

In drinking water, 18 wt. % ethanol, 5 wt. % propanol and 2 wt. acetic acid. The stimulant is injected into polluted groundwater with a higher total organic carbon content in order to change the solubility ratios of the miscible organic contaminants (benzene, toluene, xylene, ethylbenzene) in order to increase their representation in groundwater for biodegradation purposes.

Example 2

15% wt. % ethanol, 5 wt. % propanol and 2 wt. lactic acid. The stimulant is applied on the biodegradation area as part of the decontamination of sandy material and building debris in the biorermediation heap regime in order to accelerate the biodegradation of limited miscible organic contaminants (benzene, toluene, xylene, ethylbenzene) for biodegradation purposes. The stimulant is applied with a 1: 1 nutrient solution (volume ratio).

In other exemplary embodiments, the stimulant composition of the present invention may contain from 10 to 20 wt. % ethanol, 5 to 8 wt. % propanol, 0 to 5 wt. % acetic acid and 0 to 5 wt. lactic acid.

The stimulant is applied to a rock environment system with groundwater affected by benzene, toluene, xylene or ethylbenzene, where strictly anaerobic conditions prevail. Before application, it is necessary to eliminate dissolved oxygen in the water used to prepare the stimulant by boiling or displacement with an inert gas.

PROTECTION REQUIREMENTS

Claims (2)

A stimulant for stimulating biodegradation processes against poorly miscible substances of organic origin, characterized in that it consists of drinking water containing 10 to 20% by weight of water. % ethanol, 5 to 8 wt. % propanol, 0 to 5 wt. % acetic acid and 0 to 5 wt. lactic acid. Stimulant according to claim 1, characterized in that drinking water does not contain dissolved oxygen for application to the rock environment.