CZ24517U1 - Plant biostimulator - Google Patents

Description

Biostimulator of plants

Technical field

The technical solution concerns protein-based biostimulators intended for plant protection and stimulation of their growth and development.

BACKGROUND OF THE INVENTION

At present, pesticides and plant growth and development stimulators on a chemical basis are a standard part of growing technology. These, while having a good and often long-term effect on the harmful agent in question, moreover with a rapid onset of action, are a considerable burden on the environment. In addition, they can act in a non-specific manner, ie by suppressing at the same time the beneficial species that would need to be preserved in the ecosystem. There is therefore a long-term targeted effort to minimize the negative impact of pesticides with a practical impact on the so-called integrated plant protection, which aims to ensure a sufficient level of plant protection with minimal use of pesticides. In organic farming systems, the use of chemical-based products is completely prohibited. There, the only useful alternative is biopreparations.

Although biological plant protection products and the stimulation of their growth and development are increasingly used, unlike chemical-based substances, existing bio-preparations have a slow onset of action, their effect tends to be weaker, can be more expensive and often require specific application conditions . Because of these drawbacks, organic products are still used to a limited extent in conventional farming systems. Although it is known that a material on a purely natural basis is well and rapidly degradable and therefore does not pollute the environment, the higher cost of existing biological products also plays a negative role.

At the same time, it is well known that industries working with biological materials such as the tanning, footwear, food, meat and agricultural industries produce significant quantities of protein waste, which are mostly disposed of by incineration or deposited in so-called secure landfills in specific locations . This is both costly and presents a significant and growing risk to the environment.

SUMMARY OF THE INVENTION The object of the present invention is to overcome the above-mentioned disadvantages of the prior art and to utilize and effectively process the available biological material into a plant biostimulator.

The essence of the technical solution

The aforementioned drawbacks of the prior art are eliminated and the object is solved by a protein biostimulator based on protein waste according to the present invention, which consists in that it consists of hydrolyzed collagen and / or keratin raw material or a mixture of these raw materials selected from the group consisting of , tannery, meat, food, footwear or agricultural industries.

According to this invention, it is preferred that the biostimulator contains an enzyme as a hydrolysis accelerator.

Advantageously, the biostimulator comprises 20 to 40% by weight of dry matter and 14 to 17% by weight of organic nitrogen in the dry matter.

The biostimulator may also contain potassium or phosphorus and nitrogen as added during the hydrolysis.

Hydrolysed collagen and / or the keratin material is then preferably have a molecular weight from 3000 to 100,000 gmol ^first

The proposed biostimulator may also have the addition of starch dialdehyde.

-1 CZ 24517 Ul

The transformation of hazardous wastes from the tannery, leather, footwear, food, meat and agricultural industries into a biostimulator of plants eliminates the possibility of environmental pollution by biowaste, while providing an effective biological plant protection product under favorable conditions and stimulating plant growth and development.

Examples

Example 1 Pure hydrolyzate

Proteolytic enzyme catalyzed alkaline hydrolysis product by isopropylamine.

The starting material is the commercial hydrolyzate HYKOL-E STOSPOL produced by alkaline dechroi tation of blanks, which is a waste of the tanning industry, catalysed by a proteolytic enzyme

ALCALASEDXL manufactured by Novonordisk, Denmark, as described in Kolomaznik K., Mladek M., Langmaier F., Janacova D., Experience in industrial practice of enzymatic dechromation of chrome shavings, JALCA. 1999; 94: 55-63.

Composition: organic nitrogen 18%

CaO (calcium oxide) 0.5%

Mw = 5500 g.mol ^-1 .

Example 2

Hydrolyzed waste collagen CO

The starting material for the hydrolysis is waste collagen enzymatically hydrolyzed using cis 20 propylamine containing organically bound sulfur.

Mn = 7900 g / mol ¹ ; Mw = 21800 g.mol ^-1 .

Example 3

Keratin hydrolyzate K1

Mn = 3100 g / mol ¹ ; Mw = 21800 g.mol * ¹ .

Example 4

Hydrolyzed waste collagen C2 Mn - 1500 g.mol ^-1 ; Mw = 3200 g.mol ^-1 .

Example 5

Increase of seed yield in winter rape

The biological hydrolyzate of Example 1 was applied by foliar spraying of the winter rape variety Ορο · nent in the spring in the sustained growth phase at a rate of 5 L of hydrolyzate + 300 L of water per hectare. This application increased seed yield by 7.6%.

Example 6

Increase of seed yield in winter rape

The biological hydrolyzate of Example 1 was applied by foliar spraying of the winter rape variety Execu tive in the spring in the sustained growth phase at a rate of 5 l of hydrolyzate + 300 l of water per hectare. This application increased seed yield by 10.4%.

-1 CZ 24517 Ul

Example 7

Improving the health of winter rape

The biological hydrolyzate of Example 1 was applied by foliar spraying of an Ontario winter rape variety in the spring growth phase at a rate of 5 L hydrolyzate + 300 L water per hectare. This application reduced the proportion of plants infected with Sclerotinia sclerotiorum from 30% in the untreated control to 10 to 15% in the hydrolyzate treated variants.

The experiments in examples 5, 6, 7 were based on EPPO methodologies (www.eppo.org):

PP 1/135 (3) Phytotoxicity assessment

PP1 / 152 (3) Design and analysis of efficacy evaluation trials to PP1 / 181 (3) Conduct and reporting of efficacy evaluation trials including good experimental PP1 / 153 (2) Guideline for Efficiency Evaluation of Plant Growth Regulators. Control of accommodation and handling of canopy structure in rape.

Example 8

Induction of disease resistance in winter rape under laboratory conditions i The biological hydrolysates of Example 2 (collagen hydrolyzate) and 3 (keratin hydrolyzate) were applied by spraying on the uterine leaves of the Columbus winter rape. The result was the induction of resistance in the uterine leaves against the causative agent of the foamy rot, the fungal pathogen Leptosphaeria maculans. Spraying the uterine leaves with a 2% hydrolyzate solution reduces the development of disease symptoms (necrosis) on the uterine leaves by up to 61% (keratin hydrolyzate K.1) and 40% (hydrolyzed ko20 lagen) compared to a water-treated control. In view of this, it is expected that collagen hydrolyzate will also be effective against other biotrophic rape pathogens, including Pyrenopeziza brassicae and Peronospora parasitica.

Example 9

Induction of salicylic acid signaling pathway and resistance in Arabidopsis thaliana plants under labo25 conditions

Application of the biological hydrolyzate of Example 4 (hydrolyzate C2) at a concentration of 2% by foliar spray induces a salicylic acid signaling pathway in A. thaliana plants that triggers plant defense mechanisms. This activation resulted in a complete cessation of infection development in Pseudomonas syringae leaves.

He did

Antifungal effect

Hydrolysates of collagen of Example 2 and keratin of Example 3 also exhibit an antifungal effect. In vitro culture slows the growth of L. maculans mycelium at a concentration of 0.016% and higher.

Example 11

Increased resistance to powdery mildew in winter wheat

The effects of the biological hydrolyzate of Example 1 on the occurrence of powdery mildew (Blumeria graminis f.sp. tritici) on winter wheat were investigated in small-plot trials in 2009 and 2010. The plants were sprayed three times with hydrolyzate. In all observed years, spraying hydrolyzates had a protective effect and reduced the occurrence of powdery mildew by up to 65% compared to the untreated control.

Claims (7)

PROTECTION REQUIREMENTS 1. A protein biostimulator based on proteinaceous waste, characterized in that it consists of hydrolysed collagenous and / or keratin raw material or a mixture of these raw materials from the group comprising waste of leather, tanneries, meat, food, footwear 5 or the agricultural industry. Biostimulator according to claim 1, characterized in that the hydrolyzed feedstock comprises an enzyme used as a hydrolysis accelerator. Biostimulator according to claim 1 or 2, characterized in that it contains 20 to 40% by weight of dry matter and 14 to 17% by weight of organic nitrogen in the dry matter. Biostimulator according to claims 1 to 3, characterized in that it contains potassium as a supplement. Biostimulator according to claims 1 to 4, characterized in that it contains phosphorus and preferably nitrogen added during the hydrolysis. Biostimulator according to claims 1 to 5, characterized in that the hydrolyzed co-15 lagenic and / or keratin raw material has a molecular weight in the range from 3000 to 100000 g.mol ^-1 . A biostimulator according to claims 1 to 6, characterized in that it comprises the addition of starch dialdehyde.