CY1110678T1 - MATERIALS AND METHODS FOR TRADING PATHOGENES AND DETERMINATION OF CHRYSINOTRIC CARBONIC ACID FROM A SAMPLE - Google Patents

MATERIALS AND METHODS FOR TRADING PATHOGENES AND DETERMINATION OF CHRYSINOTRIC CARBONIC ACID FROM A SAMPLE

Info

Publication number
CY1110678T1
CY1110678T1 CY20091100540T CY091100540T CY1110678T1 CY 1110678 T1 CY1110678 T1 CY 1110678T1 CY 20091100540 T CY20091100540 T CY 20091100540T CY 091100540 T CY091100540 T CY 091100540T CY 1110678 T1 CY1110678 T1 CY 1110678T1
Authority
CY
Cyprus
Prior art keywords
methods
fibrin
sample
materials
plasminogen
Prior art date
Application number
CY20091100540T
Other languages
Greek (el)
Inventor
Matt Ewert
Phil Amuso
Andrew Cannons
Original Assignee
University Of South Florida
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from US10/604,779 external-priority patent/US7998699B2/en
Priority claimed from EP04801986A external-priority patent/EP1654387B1/en
Application filed by University Of South Florida filed Critical University Of South Florida
Publication of CY1110678T1 publication Critical patent/CY1110678T1/en

Links

Classifications

    • Y02P70/54—

Landscapes

  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Other Investigation Or Analysis Of Materials By Electrical Means (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Sampling And Sample Adjustment (AREA)

Abstract

Η παρούσα εφεύρεση αφορά σε μεθόδους και υλικά για την εκχύλιση λοιμωδών παθογόνων από ένα δείγμα, όπως το αίμα, που περιλαμβάνουν τα βήματα δημιουργίας συσσωματώματος ινώδους που εγκλείει τα παθογόνα και εισαγωγής αντιδραστηρίου λύσεως του ινώδους για να εκθέσει τα παθογόνα για ανάλυση. Το αντιδραστήριο λύσεως του ινώδους κατά προτίμηση συντίθεται από πλασμινογόνο και στρεπτοκινάση κατεψυγμένα σε συμπίπτουσα σχέση μέχρι να χρειαστεί το αντιδραστήριο λύσεως του ινώδους δια του οποίου η στρεπτοκινάση αντιδρά ενζυμικά με το πλασμινογόνο για να σχηματίσει πλασμίνη μετά από απόψυξη. Κατά προτίμηση, το πλασμινογόνο εναιωρείται εντός υδατικού αλατούχου διαλύματος προ της καταψύξεως συμπεριλαμβανομένων NaCI και Na3PO4. Η παρούσα εφεύρεση αφορά επίσης σε υλικά και μεθόδους για την αποτελεσματική απομάκρυνση του ΑΤΑ από ένα δείγμα που περιλαμβάνει σύνθεση νουκλεϊνικού οξέος. Οι παρούσες μέθοδοι παρέχουν σύνθεση νουκλεϊνικού οξέος απαλλαγμένη επαρκώς από ΑΤΑ ώστε να μπορεί να πραγματοποιηθεί μία αντίδραση RT-PCR και άλλες αντιδράσεις που εμπλέκουν αντίστροφη μεταγραφάση.The present invention relates to methods and materials for the extraction of infectious pathogens from a sample, such as blood, comprising the steps of creating a fibrin cluster that encompasses the pathogens and introducing a fibrin lysis reagent to expose the pathogens for analysis. The fibrin lysing reagent is preferably composed of plasminogen and streptokinase frozen in a coincidental relationship until the fibrin lysing agent through which streptokinase reacts enzymatically with the plasminogen to form plasmid is required. Preferably, the plasminogen is suspended in an aqueous saline prior to freezing including NaCl and Na3PO4. The present invention also relates to materials and methods for effectively removing ATA from a sample comprising nucleic acid synthesis. The present methods provide nucleic acid synthesis sufficiently free of ATA so that an RT-PCR reaction and other reactions involving reverse transcriptase can be performed.

CY20091100540T 2003-08-15 2009-05-21 MATERIALS AND METHODS FOR TRADING PATHOGENES AND DETERMINATION OF CHRYSINOTRIC CARBONIC ACID FROM A SAMPLE CY1110678T1 (en)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US10/604,779 US7998699B2 (en) 2002-08-15 2003-08-15 Early detection of pathogens in blood
US48189204P 2004-01-14 2004-01-14
EP04801986A EP1654387B1 (en) 2003-08-15 2004-08-16 Materials and methods for capture of pathogens and removal of aurintricarboxylic acid from a sample

Publications (1)

Publication Number Publication Date
CY1110678T1 true CY1110678T1 (en) 2015-06-10

Family

ID=47087674

Family Applications (2)

Application Number Title Priority Date Filing Date
CY20091100540T CY1110678T1 (en) 2003-08-15 2009-05-21 MATERIALS AND METHODS FOR TRADING PATHOGENES AND DETERMINATION OF CHRYSINOTRIC CARBONIC ACID FROM A SAMPLE
CY20111100803T CY1112188T1 (en) 2003-08-15 2011-08-22 MATERIALS AND METHODS FOR TRADING PATHOGENES AND DETERMINATION OF CHRYSINOTRIC CARBONIC ACID FROM A SAMPLE

Family Applications After (1)

Application Number Title Priority Date Filing Date
CY20111100803T CY1112188T1 (en) 2003-08-15 2011-08-22 MATERIALS AND METHODS FOR TRADING PATHOGENES AND DETERMINATION OF CHRYSINOTRIC CARBONIC ACID FROM A SAMPLE

Country Status (3)

Country Link
JP (3) JP2013005820A (en)
CY (2) CY1110678T1 (en)
HK (2) HK1136317A1 (en)

Families Citing this family (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2014167678A1 (en) * 2013-04-10 2014-10-16 三菱電機株式会社 Control device for permanent magnet motor
CN104458969B (en) * 2014-12-31 2016-05-11 深圳康泰生物制品股份有限公司 The assay method of Triton X-100 residual quantity in the HBsAg stoste that recombinant Saccharomyces cerevisiae is expressed

Family Cites Families (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DK1654387T3 (en) * 2003-08-15 2009-06-22 Univ South Florida Materials and Methods for Capturing Pathogens and Removing aurintricarboxylic Acid from a Sample

Also Published As

Publication number Publication date
HK1136317A1 (en) 2010-06-25
HK1136852A1 (en) 2010-07-09
JP2013005819A (en) 2013-01-10
CY1112188T1 (en) 2015-12-09
JP2013005820A (en) 2013-01-10
JP2013039134A (en) 2013-02-28

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