CS274948B2 - Method of chromolytic substrate preparation for inulinasa's enzyme activity determination - Google Patents
Method of chromolytic substrate preparation for inulinasa's enzyme activity determinationInfo
- Publication number
- CS274948B2 CS274948B2 CS223288A CS223288A CS274948B2 CS 274948 B2 CS274948 B2 CS 274948B2 CS 223288 A CS223288 A CS 223288A CS 223288 A CS223288 A CS 223288A CS 274948 B2 CS274948 B2 CS 274948B2
- Authority
- CS
- Czechoslovakia
- Prior art keywords
- inuline
- preparation
- enzyme activity
- chromolytic
- solution
- Prior art date
Links
- 238000002360 preparation method Methods 0.000 title abstract 4
- 230000000694 effects Effects 0.000 title abstract 3
- 239000000758 substrate Substances 0.000 title abstract 3
- 108090000790 Enzymes Proteins 0.000 title abstract 2
- 102000004190 Enzymes Human genes 0.000 title abstract 2
- 238000000034 method Methods 0.000 title abstract 2
- NNDHDYDFEDRMGH-CAEIVAEBSA-N Anthranoyllycoctonine Chemical compound C([C@]12CN(C3[C@@]4(O)[C@]5(O)[C@H]6[C@@H](OC)[C@@H]([C@H](C5)OC)C[C@H]6[C@@]3([C@@H]1[C@@H]4OC)[C@@H](OC)CC2)CC)OC(=O)C1=CC=CC=C1N NNDHDYDFEDRMGH-CAEIVAEBSA-N 0.000 abstract 4
- UXOXDDUEWZOAIW-UHFFFAOYSA-N Inuline Natural products CCN1CC2(CC(=O)Oc3ccccc3N)CCC(OC)C45C6CC7C(CC(O)(C6C7OC)C(O)(C(OC)C24)C15)OC UXOXDDUEWZOAIW-UHFFFAOYSA-N 0.000 abstract 4
- VNRZCPPHNPEBFC-UHFFFAOYSA-N anthranoyllycoctonine Natural products CCN1CC2(COC(=O)c3ccccc3N)CCC(OC)C45C2C(OC)C(O)(C14)C6(O)CC(OC)C7CC5(O)C6C7OC VNRZCPPHNPEBFC-UHFFFAOYSA-N 0.000 abstract 4
- OKKJLVBELUTLKV-UHFFFAOYSA-N Methanol Chemical compound OC OKKJLVBELUTLKV-UHFFFAOYSA-N 0.000 abstract 3
- KWYUFKZDYYNOTN-UHFFFAOYSA-M Potassium hydroxide Chemical compound [OH-].[K+] KWYUFKZDYYNOTN-UHFFFAOYSA-M 0.000 abstract 3
- HEMHJVSKTPXQMS-UHFFFAOYSA-M Sodium hydroxide Chemical compound [OH-].[Na+] HEMHJVSKTPXQMS-UHFFFAOYSA-M 0.000 abstract 3
- 108010090785 inulinase Proteins 0.000 abstract 3
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 abstract 2
- WCUXLLCKKVVCTQ-UHFFFAOYSA-M Potassium chloride Chemical compound [Cl-].[K+] WCUXLLCKKVVCTQ-UHFFFAOYSA-M 0.000 abstract 2
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 abstract 2
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 abstract 2
- LSNNMFCWUKXFEE-UHFFFAOYSA-M Bisulfite Chemical compound OS([O-])=O LSNNMFCWUKXFEE-UHFFFAOYSA-M 0.000 abstract 1
- 244000298479 Cichorium intybus Species 0.000 abstract 1
- 235000007542 Cichorium intybus Nutrition 0.000 abstract 1
- QXNVGIXVLWOKEQ-UHFFFAOYSA-N Disodium Chemical class [Na][Na] QXNVGIXVLWOKEQ-UHFFFAOYSA-N 0.000 abstract 1
- BRLQWZUYTZBJKN-UHFFFAOYSA-N Epichlorohydrin Chemical compound ClCC1CO1 BRLQWZUYTZBJKN-UHFFFAOYSA-N 0.000 abstract 1
- 240000008892 Helianthus tuberosus Species 0.000 abstract 1
- 235000003230 Helianthus tuberosus Nutrition 0.000 abstract 1
- 125000002490 anilino group Chemical group [H]N(*)C1=C([H])C([H])=C([H])C([H])=C1[H] 0.000 abstract 1
- 238000004040 coloring Methods 0.000 abstract 1
- 238000004132 cross linking Methods 0.000 abstract 1
- RTZKZFJDLAIYFH-UHFFFAOYSA-N ether Substances CCOCC RTZKZFJDLAIYFH-UHFFFAOYSA-N 0.000 abstract 1
- 238000000605 extraction Methods 0.000 abstract 1
- 230000000813 microbial effect Effects 0.000 abstract 1
- 239000001103 potassium chloride Substances 0.000 abstract 1
- 235000011164 potassium chloride Nutrition 0.000 abstract 1
- 238000001556 precipitation Methods 0.000 abstract 1
- 239000011780 sodium chloride Substances 0.000 abstract 1
- 239000000725 suspension Substances 0.000 abstract 1
Landscapes
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Abstract
The solution concerns a method of preparation of chromolytic substrate for definition of inulinase enzyme activity by extraction of cut roots of chicory or Jerusalem artichokes by water surplus at a temperature of 80 to 90 degrees C, precipitation of extracted inuline from the water solution by surplus /double or triple/ of methanol or ethanol and cross linking of inuline in the originated suspension by 2,2-bis/oxiranylmethyl/-ether or 2-chloromethyloxirane in the environment of alkalis, and subsequent covalent colouring of the cross-linked inuline gel in presence of disodium salt of 8-amino-5-[-3/sulfoethylsulfonyl/anilino]-6-anthraquinone sulfonic acid, sodium hydroxide or potassium hydroxide and sodium chloride or potassium chloride.The solution can be used in enzyme engineering, in preparation of radiation-diffusive earths for selection of microbial producers of inulinase enzyme and in preparation of tablet monotests with chromolytic inuline as substrate for standard definition of inulinase enzyme activity.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CS223288A CS274948B2 (en) | 1988-04-01 | 1988-04-01 | Method of chromolytic substrate preparation for inulinasa's enzyme activity determination |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CS223288A CS274948B2 (en) | 1988-04-01 | 1988-04-01 | Method of chromolytic substrate preparation for inulinasa's enzyme activity determination |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CS8802232A1 CS8802232A1 (en) | 1990-10-12 |
| CS274948B2 true CS274948B2 (en) | 1991-12-17 |
Family
ID=5358846
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CS223288A CS274948B2 (en) | 1988-04-01 | 1988-04-01 | Method of chromolytic substrate preparation for inulinasa's enzyme activity determination |
Country Status (1)
| Country | Link |
|---|---|
| CS (1) | CS274948B2 (en) |
Families Citing this family (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| RU2385882C1 (en) * | 2009-04-06 | 2010-04-10 | Олег Иванович Квасенков | Method of preparing inulin-containing solution |
-
1988
- 1988-04-01 CS CS223288A patent/CS274948B2/en unknown
Also Published As
| Publication number | Publication date |
|---|---|
| CS8802232A1 (en) | 1990-10-12 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| KITAMURA et al. | Lysis of viable yeast cells by enzymes of Arthrobacter luteus II. Purification and properties of an enzyme, zymolyase, which lyses viable yeast cells | |
| DE3689017D1 (en) | Oligosaccharides, usable as hair restorer. | |
| McCleary et al. | Galactomannan utilization in germinating legume seeds | |
| PT81655B (en) | PROCESS OF PREPARATION OF GEL SYSTEMS USED IN FOOD | |
| SE8603008D0 (en) | SULPHATED POLYSACCARIDES AND METHOD OF PREPARING THESE | |
| Winkelmann et al. | A new myosin fragment: visualization of the regulatory domain | |
| JPS57144298A (en) | 7-beta-d-arabinofuranosyl-7h- pyrrolo(2,3-d)pyrimidine compound and manufacture | |
| Shaw et al. | Response:" Galactose Dehydrogenase,"" Nothing Dehydrogenase," and Alcohol Dehydrogenase: Interrelation | |
| Walker | Preparation of isomaltose oligosaccharides labelled with 14C in the non-reducing terminal unit, and their use in studies of dextranase activity | |
| CS274948B2 (en) | Method of chromolytic substrate preparation for inulinasa's enzyme activity determination | |
| JPS5746999A (en) | Beta-d-arabinofuranosylimidazo(4,5-c)pyridine compound and manufacture | |
| Gupta et al. | Glucofructosan biosynthesis in Fusarium oxysporum | |
| Risteli et al. | Affinity chromatography of collagen glycosyltransferases on collagen linked to agarose | |
| Goudsmit et al. | Enzymatic synthesis of galactogen in the snall, Helix, pomatia | |
| GB1492959A (en) | Process for obtaining a plasminogen activator | |
| EP0213717A3 (en) | Iodophor containing suppository | |
| GB1438919A (en) | Compositions comprising chelated radioactive heave metals | |
| ES8200723A1 (en) | Preparation of 2-keto-L-gulonic acid. | |
| GB1135354A (en) | Preparation of uricase | |
| JPS5639013A (en) | Ophthalmic solution for regulating intraocular tension | |
| DK251284A (en) | SUBSTITUTED 1,2,3-TRIAZINO (4 ', 5'.4,5) THIENO (2,3-B) -QUINOLIN-4 (3H) -ONES OR ACID ADDITION SALTS THEREOF AND THEIR PREPARATION | |
| Hardingham et al. | Studies on the incorporation of [U-14C] glucose and [35S] sulphate into the acid glycosaminoglycans of neonatal rat skin | |
| PL325833A1 (en) | Method of obtaining cation-exchanger membranes upgraded by addition of not-dissolving metal salts | |
| RUAN et al. | Development and application of anti-adherent evaluation model of H pylori adherent to MKN45 cell | |
| Beutling et al. | Histamine formation by Citrobacter freundii- its dependence on temperature, pH, und sodium chloride. |