CS270359B1 - A method for increasing avidin content in aqueous solution - Google Patents

A method for increasing avidin content in aqueous solution Download PDF

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Publication number
CS270359B1
CS270359B1 CS877881A CS788187A CS270359B1 CS 270359 B1 CS270359 B1 CS 270359B1 CS 877881 A CS877881 A CS 877881A CS 788187 A CS788187 A CS 788187A CS 270359 B1 CS270359 B1 CS 270359B1
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CS
Czechoslovakia
Prior art keywords
aqueous solution
avidin
content
increasing
lysozyme
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CS877881A
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Czech (cs)
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CS788187A1 (en
Inventor
Hana Rndr Karaskova
Marcela Aussenbergova
Jiri Rndr Navratil
Bohuslava Placha
Frantisek Ing Csc Cakl
Kamila Ing Csc Mikova
Original Assignee
Hana Rndr Karaskova
Marcela Aussenbergova
Jiri Rndr Navratil
Bohuslava Placha
Frantisek Ing Csc Cakl
Kamila Ing Csc Mikova
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Application filed by Hana Rndr Karaskova, Marcela Aussenbergova, Jiri Rndr Navratil, Bohuslava Placha, Frantisek Ing Csc Cakl, Kamila Ing Csc Mikova filed Critical Hana Rndr Karaskova
Priority to CS877881A priority Critical patent/CS270359B1/en
Publication of CS788187A1 publication Critical patent/CS788187A1/en
Publication of CS270359B1 publication Critical patent/CS270359B1/en

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Abstract

Účelem řešeni js zvýšeni obsahu avidinu v promývacim vodném roztoku používaném k vymýváni iontoměničů při výrobě lysozymu, aby se snížily náklady na následnou izolaci avidinu. Uvedeného účelu se dosáhne tim, že promývací vodný roztok ss aplikuje vícenásobně. Aplikace ee ukonči před dosažením takové koncentrace balastqich látsk, která ohrožuje kvalitu získávaného lysozymu. Takto obohacený promývaci vodný roztok se zahusti ultrafiltraci do obsahu bílkovin 8 až 18 % hmot.The purpose of the solution is to increase the avidin content in the washing aqueous solution used for washing out ion exchangers in the production of lysozyme in order to reduce the costs of subsequent avidin isolation. The stated purpose is achieved by applying the washing aqueous solution multiple times. The application is terminated before reaching such a concentration of ballast substances that endangers the quality of the obtained lysozyme. The thus enriched washing aqueous solution is concentrated by ultrafiltration to a protein content of 8 to 18% by weight.

Description

Vynález se týká způsobu zvýšeni obsahu avidinu ve vodnám roztoku používaném k vymýváni iontomšničů při výrobě lyeozymu.The present invention relates to a process for increasing the avidin content of aqueous solutions used to elute ion exchangers in the manufacture of lyeozyme.

K vymýváni iontomšničů při výrobě lysozymu ee v běžné praxi používá roztok o vhodné iontová sile pouze jednou a je po odděleni lyaozymu nahrazen novým. Izolace avidinu z tohoto roztoku je náročná časově i ekonomicky z důvodu velmi nízkého obsahu avodinu, který z vaječného bilku přejde do promývaciho roztoku - cca 30 až 50 mg v 1 1.In order to elute ion exchangers in the production of lysozyme, ee normally uses a solution of suitable ionic strength only once and is replaced by a new one after lyaozyme separation. Isolation of avidin from this solution is demanding in terms of time and economy due to the very low avodine content that passes from the egg white into the wash solution - about 30 to 50 mg in 1 L.

Tyto nevýhody odstraňuje způsob podlá vynálezu, jehož podstata spočívá v tom, že promývací vodný roztok používaný k vymýváni iontoměničů se aplikuje vícenásobně. Tim se dosáhne několikanásobného obohaceni roztoku avidinem - na cca 200 až 400 mg vil. Současně však narůstá i koncentrace dalších balastnich bílkovin. Z toho důvodu je nutná ukončit vícenásobnou aplikaci vodného roztoku před dosažením hraniční koncentrace balastnich bílkovin, která by jinak zhoršovaly kvalitu získávaného lysozymu. Takto obohacený roztok lze zahustit ultrafiltracl 5 až 6 krát k dosaženi ještš vyšší koncentrace avidinu.These drawbacks are overcome by the method of the invention, which consists in applying the wash aqueous solution used to wash the ion exchangers multiple times. This achieves multiple enrichment of the solution with avidin - to about 200 to 400 mg villas. At the same time, the concentration of other ballast proteins also increases. For this reason, it is necessary to stop multiple application of the aqueous solution before reaching the limit concentration of ballast proteins, which would otherwise impair the quality of the lysozyme obtained. The enriched solution can be concentrated by ultrafiltration 5 to 6 times to achieve an even higher concentration of avidin.

Výhodou navrženého způsobu Je to, že avldin lze takto izolovat e nižšími náklady, než z původního vymývaciho roztoku.An advantage of the proposed method is that avldin can thus be isolated at a lower cost than from the original wash solution.

Přiklad:Example:

lontoměnič Je promýván roztokem KCl o. koncentraci 6 až 12 % hmot., po dobu cca 60 min. Následuje úprava pH na 9,5'až 10,5 za účelem krystalizace lysozymu. Po 3 až 5 dnech se oddělí na filtru lysozym a zbylý promývací vodný roztok ee použije opět k vymyti iontoměniče. Toto se opakuje 20 až 25 krát - období cca 2 až 3 měsíce. Koncentrace bílkovin ve filtrátu - promývacim vodném roztoku - je cca 2 % hmot. Takto obohacený promývací vodný roztok ee β výhodou zahušřuje ultrafiltreci tak, aby obsah bílkovin byl cca 9 až 10 % hmot.Ion exchanger It is washed with a solution of KCl at a concentration of 6 to 12% by weight, for about 60 min. The pH is then adjusted to 9.5 to 10.5 to crystallize lysozyme. After 3-5 days, the lysozyme is collected on the filter and the remaining aqueous wash solution is reused to wash the ion exchanger. This is repeated 20 to 25 times - a period of about 2 to 3 months. The protein concentration in the filtrate - washing aqueous solution - is about 2 wt. The enriched washing aqueous solution ee β advantageously thickenes ultrafiltration so that the protein content is about 9 to 10% by weight.

Claims (1)

1. Způaob zvýšeni obsahu avidinu v promývacim vodném roztoku, používaném k vymýváni iontomšničů při výrobě lyeozymu, vyznačující aa tim, ža promývací vodný roztok se aplikuje opakovaná, e výhodou 20 až 25 krát a obohacený promývací vodný roztok ee zahusti ultrafiltreci do obsahu bílkovin 8 až 18 %,hmot.A method for increasing the avidin content of a wash aqueous solution used to wash ion exchangers in the manufacture of lyeozyme, characterized in that the wash aqueous solution is applied repeatedly, preferably 20 to 25 times, and the enriched wash aqueous solution is concentrated by ultrafiltration to a protein content of 8 to 18%, wt.
CS877881A 1987-11-04 1987-11-04 A method for increasing avidin content in aqueous solution CS270359B1 (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
CS877881A CS270359B1 (en) 1987-11-04 1987-11-04 A method for increasing avidin content in aqueous solution

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Application Number Priority Date Filing Date Title
CS877881A CS270359B1 (en) 1987-11-04 1987-11-04 A method for increasing avidin content in aqueous solution

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CS788187A1 CS788187A1 (en) 1989-11-14
CS270359B1 true CS270359B1 (en) 1990-06-13

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