CS270359B1 - Method of avidin content increase in aqueous solution - Google Patents
Method of avidin content increase in aqueous solution Download PDFInfo
- Publication number
- CS270359B1 CS270359B1 CS877881A CS788187A CS270359B1 CS 270359 B1 CS270359 B1 CS 270359B1 CS 877881 A CS877881 A CS 877881A CS 788187 A CS788187 A CS 788187A CS 270359 B1 CS270359 B1 CS 270359B1
- Authority
- CS
- Czechoslovakia
- Prior art keywords
- aqueous solution
- avidin
- washing
- lysozyme
- wash
- Prior art date
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- 239000007864 aqueous solution Substances 0.000 title claims abstract description 12
- 108090001008 Avidin Proteins 0.000 title claims abstract description 8
- 238000000034 method Methods 0.000 title claims description 5
- 238000004519 manufacturing process Methods 0.000 claims abstract description 4
- 238000000108 ultra-filtration Methods 0.000 claims abstract description 4
- 102000016943 Muramidase Human genes 0.000 abstract description 6
- 108010014251 Muramidase Proteins 0.000 abstract description 6
- 108010062010 N-Acetylmuramoyl-L-alanine Amidase Proteins 0.000 abstract description 6
- 229960000274 lysozyme Drugs 0.000 abstract description 6
- 235000010335 lysozyme Nutrition 0.000 abstract description 6
- 239000004325 lysozyme Substances 0.000 abstract description 6
- 238000005406 washing Methods 0.000 abstract description 5
- 238000002955 isolation Methods 0.000 abstract description 2
- 239000000126 substance Substances 0.000 abstract 1
- 239000000243 solution Substances 0.000 description 8
- 108090000623 proteins and genes Proteins 0.000 description 3
- 102000004169 proteins and genes Human genes 0.000 description 3
- QCVGEOXPDFCNHA-UHFFFAOYSA-N 5,5-dimethyl-2,4-dioxo-1,3-oxazolidine-3-carboxamide Chemical compound CC1(C)OC(=O)N(C(N)=O)C1=O QCVGEOXPDFCNHA-UHFFFAOYSA-N 0.000 description 1
- 108010000912 Egg Proteins Proteins 0.000 description 1
- 102000002322 Egg Proteins Human genes 0.000 description 1
- 238000010936 aqueous wash Methods 0.000 description 1
- 235000014103 egg white Nutrition 0.000 description 1
- 210000000969 egg white Anatomy 0.000 description 1
- 239000000706 filtrate Substances 0.000 description 1
- 238000000926 separation method Methods 0.000 description 1
Landscapes
- Peptides Or Proteins (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
Abstract
Účelem řešeni js zvýšeni obsahu avidinu v promývacim vodném roztoku používaném k vymýváni iontoměničů při výrobě lysozymu, aby se snížily náklady na následnou izolaci avidinu. Uvedeného účelu se dosáhne tim, že promývací vodný roztok ss aplikuje vícenásobně. Aplikace ee ukonči před dosažením takové koncentrace balastqich látsk, která ohrožuje kvalitu získávaného lysozymu. Takto obohacený promývaci vodný roztok se zahusti ultrafiltraci do obsahu bílkovin 8 až 18 % hmot.The purpose of dealing with content enhancement avidin in the washing aqueous solution used to wash out ion exchangers in production lysozyme to reduce cost subsequent isolation of avidin. That purpose is achieved by washing the washing aqueous solution ss applies multiple times. Exit ee before reaching such a balastqich concentration substance that threatens quality lysozyme obtained. Washed in this way the aqueous solution is concentrated by ultrafiltration up to a protein content of 8 to 18 wt.
Description
Vynález se týká způsobu zvýšeni obsahu avidinu ve vodnám roztoku používaném k vymýváni iontomšničů při výrobě lyeozymu.The present invention relates to a process for increasing the avidin content of aqueous solutions used to elute ion exchangers in the manufacture of lyeozyme.
K vymýváni iontomšničů při výrobě lysozymu ee v běžné praxi používá roztok o vhodné iontová sile pouze jednou a je po odděleni lyaozymu nahrazen novým. Izolace avidinu z tohoto roztoku je náročná časově i ekonomicky z důvodu velmi nízkého obsahu avodinu, který z vaječného bilku přejde do promývaciho roztoku - cca 30 až 50 mg v 1 1.In order to elute ion exchangers in the production of lysozyme, ee normally uses a solution of suitable ionic strength only once and is replaced by a new one after lyaozyme separation. Isolation of avidin from this solution is demanding in terms of time and economy due to the very low avodine content that passes from the egg white into the wash solution - about 30 to 50 mg in 1 L.
Tyto nevýhody odstraňuje způsob podlá vynálezu, jehož podstata spočívá v tom, že promývací vodný roztok používaný k vymýváni iontoměničů se aplikuje vícenásobně. Tim se dosáhne několikanásobného obohaceni roztoku avidinem - na cca 200 až 400 mg vil. Současně však narůstá i koncentrace dalších balastnich bílkovin. Z toho důvodu je nutná ukončit vícenásobnou aplikaci vodného roztoku před dosažením hraniční koncentrace balastnich bílkovin, která by jinak zhoršovaly kvalitu získávaného lysozymu. Takto obohacený roztok lze zahustit ultrafiltracl 5 až 6 krát k dosaženi ještš vyšší koncentrace avidinu.These drawbacks are overcome by the method of the invention, which consists in applying the wash aqueous solution used to wash the ion exchangers multiple times. This achieves multiple enrichment of the solution with avidin - to about 200 to 400 mg villas. At the same time, the concentration of other ballast proteins also increases. For this reason, it is necessary to stop multiple application of the aqueous solution before reaching the limit concentration of ballast proteins, which would otherwise impair the quality of the lysozyme obtained. The enriched solution can be concentrated by ultrafiltration 5 to 6 times to achieve an even higher concentration of avidin.
Výhodou navrženého způsobu Je to, že avldin lze takto izolovat e nižšími náklady, než z původního vymývaciho roztoku.An advantage of the proposed method is that avldin can thus be isolated at a lower cost than from the original wash solution.
Přiklad:Example:
lontoměnič Je promýván roztokem KCl o. koncentraci 6 až 12 % hmot., po dobu cca 60 min. Následuje úprava pH na 9,5'až 10,5 za účelem krystalizace lysozymu. Po 3 až 5 dnech se oddělí na filtru lysozym a zbylý promývací vodný roztok ee použije opět k vymyti iontoměniče. Toto se opakuje 20 až 25 krát - období cca 2 až 3 měsíce. Koncentrace bílkovin ve filtrátu - promývacim vodném roztoku - je cca 2 % hmot. Takto obohacený promývací vodný roztok ee β výhodou zahušřuje ultrafiltreci tak, aby obsah bílkovin byl cca 9 až 10 % hmot.Ion exchanger It is washed with a solution of KCl at a concentration of 6 to 12% by weight, for about 60 min. The pH is then adjusted to 9.5 to 10.5 to crystallize lysozyme. After 3-5 days, the lysozyme is collected on the filter and the remaining aqueous wash solution is reused to wash the ion exchanger. This is repeated 20 to 25 times - a period of about 2 to 3 months. The protein concentration in the filtrate - washing aqueous solution - is about 2 wt. The enriched washing aqueous solution ee β advantageously thickenes ultrafiltration so that the protein content is about 9 to 10% by weight.
Claims (1)
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CS877881A CS270359B1 (en) | 1987-11-04 | 1987-11-04 | Method of avidin content increase in aqueous solution |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CS877881A CS270359B1 (en) | 1987-11-04 | 1987-11-04 | Method of avidin content increase in aqueous solution |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CS788187A1 CS788187A1 (en) | 1989-11-14 |
| CS270359B1 true CS270359B1 (en) | 1990-06-13 |
Family
ID=5428715
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CS877881A CS270359B1 (en) | 1987-11-04 | 1987-11-04 | Method of avidin content increase in aqueous solution |
Country Status (1)
| Country | Link |
|---|---|
| CS (1) | CS270359B1 (en) |
-
1987
- 1987-11-04 CS CS877881A patent/CS270359B1/en unknown
Also Published As
| Publication number | Publication date |
|---|---|
| CS788187A1 (en) | 1989-11-14 |
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