CS268277B1 - A method for producing hydrolyzate of chelae for cosmetic and pharmaceutical purposes - Google Patents

Abstract

The purpose of the solution is to prepare a hydrolysate of chalazae from egg white for cosmetic and pharmaceutical purposes. The stated purpose is achieved by performing partial hydrolysis in an alkaline environment at pH 11 to 14 and then digestion with pepsin in a ratio of weight parts of dry substance of substrate: pepsin of 500:1 to 50:1 at a temperature of 20 °C to 22 °C at pH 1.8 to 2.2 for 7 to 10 hours. Subsequently, the pH is adjusted to 8.0 to 8.5 and then dialysis against water and centrifugation or filtration. Dehydration It is also possible to perform drying in a spray dryer at a temperature of 70 °C to 85 °C, or possibly lyophilization.

Description

BACKGROUND OF THE INVENTION The present invention relates to a process for the production of cbalazel hydrolyzate obtained from egg flour, which is used in the manufacture of cosmetic and pharmaceutical preparations, and the like. skin cleansers.

The use of chalazees, their extracts or hydrolyzates for cosmetic or pharmaceutical purposes is not yet known. According to the present invention, a odorless and colorless product is obtained which meets the stringent requirements imposed on the basic raw materials for cosmetic and pharmaceutical purposes and which is at the same time resistant to the adverse effects of storage. Use of hydrolyzate of chalazees for cosmetic and pharmaceutical purposes It is a significant benefit of the economy, because by means of the treatment described below the worthless waste was transformed into an extremely valuable raw material.

Chalase Hydrolysate It is very easy to process into external skin preparations in the form of aqueous and / or alcoholic solutions, gels, solid or liquid emulsions with an external aqueous or oily phase to treat and clean skin and hair. Chalase Hydrolysate As a component of said external formulations, it exhibits an emolientic and lubricating effect on the skin and an improvement in their mechanical-elastic properties.

SUMMARY OF THE INVENTION The invention consists in performing partial hydrolysis in an alkaline medium in a water bath at a temperature of 70 to 85 ° C, preferably 80 ° C, with stirring for 10 to 20 minutes, preferably 15 minutes, after which the solution is cooled to room temperature. . Subsequently, the pH is adjusted to an acidic reaction and pepsin digestion.

The hydrolyzate of chalazees can be produced according to the following example of a technological process, which does not limit or exhaust the scope of the invention.

Example

Eggplant-derived chalazees are continuously concentrated and stored frozen in pre-cleaned and 0.5% disinfectant Septonex disinfected containers at -18 to 28 ° C for a maximum of 90 days.

For further processing, a partial hydrolysis of the chalase in an 80 ° C water bath with an alkaline reaction is first carried out by mixing 1 part by weight of chalase dry matter with 5 parts by weight of 0.1 M NaOH followed by stirring for 15 minutes. The contents were then cooled to room temperature and the reaction was adjusted to pH 2.0-0.1 by sequential addition of 6 N HCl. Pepsin was then added at a rate of 10 mg of pepsin per 1.0 g of initial substrate dry weight. The reaction mixture is stirred at 21-1 ° C for 8 hours during which the pepsin hydrolysis proceeds. After this time, the pepsin degeneration is stopped by adjusting the pH with 4 M NaOH so that the reaction mixture has a pH of 8.2-0.2. The solution is centrifuged for 1 hour or filtered, dialyzed against water for 3 days and dehydrated by means of a spray drier, possibly by lyophilization.

Dehydrated product Adjusted to perfectly sealed glass or plastic containers.

The yield of this process is about 99% of the starting mass of chalase.

Claims (2)

OBJECT OF THE INVENTION A process for the production of a hyphaelate hydrolyzate obtained from egg white for cosmetic and pharmaceutical purposes, characterized in that partial hydrolysis is performed in alkaline medium at pH 11 to 14, preferably 14 pH, followed by digestion with pepsin at a ratio of dry weight of substrate: pepsin 500: 1 to 50: 1, preferably 100: 1, at 20 to 22 ° C, preferably 21 ° C, at pH 1.8 to 2.2, preferably pH 2.0, for 7 to 10 hours, preferably 8 hours, whereupon the pH is adjusted to 8.0 to 8.5, preferably 8.2, followed by dialysis against water and centrifugation or filtration. 2. A process for producing a chelate hydrolyzate according to claim 1, characterized in that the dehydration is carried out in an aromatic solution at a temperature of 70 to 85 [deg.] C., preferably 00 [deg.] C., optionally by lyophilization.