CS264507B1

CS264507B1 - Device for isolation, short-term preservation and bloodless transfer of embryos in cattle

Info

Publication number: CS264507B1
Application number: CS844244A
Authority: CS
Inventors: Lubomir Doc Mvdr Csc Holy; Ladislav Ing Skarek
Original assignee: Lubomir Doc Mvdr Csc Holy; Ladislav Ing Skarek
Priority date: 1984-06-05
Filing date: 1984-06-05
Publication date: 1989-08-14
Also published as: CS424484A1

Abstract

Zařízení je určeno pro isolaci, krátkodobou konzervaci a nekrvavý přenos embryí u skotu. Zařízení obsahuje mikromanipulátor s injekční jehlou, plastickou embryační hadičku a plastický katetr. Výhodou zařízení je, že umožňuje depozici embrya do nejpříznivější hloubky děložního rohu. Tím, že je pružné, nedochází k traumatizaci děložní sliznice, čímž se zlepšují podmínky pro přijetí embrya příjemcem.The device is intended for isolation, short-term preservation and bloodless transfer of embryos in cattle. The device includes a micromanipulator with an injection needle, a plastic embryo tube and a plastic catheter. The advantage of the device is that it allows the embryo to be deposited into the most favorable depth of the uterine horn. Since it is flexible, there is no trauma to the uterine mucosa, which improves the conditions for the embryo to be accepted by the recipient.

Description

Vynález se týká zařízení pro isolaci, krátkodobou konzervaci a nekrvavý přenos embryí u skotu.BACKGROUND OF THE INVENTION The present invention relates to a device for the isolation, short-term preservation and bloodless transfer of embryos in cattle.

Dosud známá provedení zařízení k nekrvavému přenosu ranných embryí u skotu používají principu podle Cassou nebo Hahna, ostatní zařízení jsou velmi podobná. Princip Cassou používá pevné trubičky na konci se zúžením, které slouží jako zarážka. Do trubičky se před přenosem vsune hadička s embryem a nasunutým mandrenem, který slouží jako píst, po zavedení do dělohy se mandren stlačí a tím se provede depozice embrya do dělohy.The hitherto known devices for the bloodless transfer of early embryos in cattle use the principle of Cassa or Hahn, the other devices are very similar. Principle Cassou uses fixed tubes at the end with a taper that serves as a stop. A tube with an embryo and an inserted mandrel, which serves as a plunger, is inserted into the tube before transfer, and the mandren is compressed after insertion into the uterus to deposit the embryo in the uterus.

Princip podle Hahna používá rovněž pevný trubkový zavaděč, který je dvoudílný s uzavřeným koncem a bočním vývodem. Embryo se umístní do hadičky, která se nasune do koncového dílu a na druhý konec hadičky nasune mandren s druhou částí zavaděče a sešroubováním obou dílů je zařízení připraveno k depozici. Po zavedení do dělohy se stlačí mandren, který působí jako píst a embryo je deponováno do děložního prostoru.The Hahn principle also uses a solid tubular inserter, which is two-piece with closed end and side outlet. The embryo is placed in a tubing that slips into the end piece and the mandrel is inserted into the other end of the tubing with the second inserter part and the device is ready for deposition by screwing the two parts together. After insertion into the uterus, the mandren acts as a piston and the embryo is deposited in the uterine space.

Tyto systémy jsou nevýhodné zejména v tom, že nejsou elastické a dovolují přenos embrya pouze do báze děložního rohu a dále může snadno docházet k poranění sliznice, což ovlivňuje negativně přežívání embryí.These systems are particularly disadvantageous in that they are not elastic and allow embryo transfer to the uterine base only, and mucosal injury can easily occur, which adversely affects embryo survival.

Podstata zařízení pro nekrvavý přenos, isolace a krátkodobou konzervaci embryí u skotu, zaleží v tom, že jej tvoří mikromanipulátor s injekční stříkačkou, opatřenou odhrotovanou injekční jehlou, na níž je nasazena dlouhá tran-The nature of the device for bloodless transfer, isolation and short-term preservation of embryos in cattle depends on the fact that it consists of a micromanipulator with a syringe, equipped with a deburring injection needle, on which a long trance

Claims

OBJECTIVE Equipment for the isolation, short-term preservation and non-blood transfer of embryos in bovine animals, consisting of a micromanipulator, in which a plastic syringe is mounted, on which a deburring injection needle is attached, characterized by a transparent embryonating tube terminated with a non-toxic plastic capillary, in this assembly is placed in a non-toxic capillary under microscopic control. When introducing the embryo, the uterus is made accessible through a plastic catheter and, after insertion of the capillary embryo tube through the catheter into the uterus, and the micromanipulator being replaced by a syringe, deposition of the embryo into the uterine space can be accomplished by pushing the plunger. The main advantage of this assembly is that it is possible to monitor the quality of the embryo until its introduction, the traumatization of the uterus does not occur and allows the embryo to be deposited in the desired parts of the uterine horn. In the drawings, an exemplary embodiment of an assembly of an embryo force device is shown in FIG.

2 shows an example of an embodiment of a device for short-term preservation and deposition of an embryo. The embryo isolation assembly of FIG. 1 comprises a non-toxic plastic capillary 7 which is threaded into a toxic portion formed by a plastic embryonic tubing 2, at the end of which a tapered hollow injection needle 3 is threaded into which a micromanipulator 5 fixedly connected to the injection A syringe 4 is operated by a handwheel 6. For embryo deposition, the micromanipulator 5 is replaced with a syringe 4 by a syringe 8 and uterine dilation is performed by a plastic catheter 1 in that a long transparent needle is attached to the deburred end of the injection needle (3). a plastic embryo tube (2) terminates in a non-toxic plastic capillary (7) on which a plastic catheter (1) is threaded. 2 drawings IHUIIIIIIIIIII

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Fig. 2 Severografia, n. p., MOST Price 2.40 Kcs