CS258252B1 - Resource ks increase the number of cells - Google Patents

Abstract

A means for increasing the number of cells or protoplasts in tissue, cell or protoplast culture, consisting of 6-azido-6-deoxy-D-glucose, is effective especially in the second half of the cultivation cycle and is used to increase production or shorten the production cycle of biotechnological processes. Possible use in the pharmaceutical and feed industries.

Description

The invention relates to the biochemical regulation of biotechnological processes. While achieving the relative stability of biotechnological production provided by tissue, cell or protoplast cultures, it is desirable to further increase their productivity, biomass production and the number of cells recoverable during the culture cycle.

As follows from the following example, the use of 6-azido-6-deoxy-D-glucose is based on the discovery of its new property, namely the ability to increase the number of cells and / or protoplasts obtainable during the culture cycle in tissue and / or cellular and / or protoplasts. cultures. The present invention provides a means for increasing the number of protoplasts and / or cells in a tissue and / or cell and / or protoplast culture, particularly effective in the second half of the culture cycle, characterized in that it is composed of 6-azido-6-deoxy-D-glucose.

This also increases the biomass production, or the possibility to achieve the same production in a shorter time.

Example

Aqueous solutions of 6-azido-6-deoxy-D-glucose were added to the standard suspension cell culture solution optimized for Papaver somniferum (opium alkaloid producer) cells before inoculation. The initial cell concentration when inoculated was 70,000 cells per ml suspension. The experimental passage, i.e. the length of the culture cycle, lasted 15 days. The following table shows the number of cells per 1 susp suspension depending on the time elapsed since the beginning of the cultivation:

Table

Number of days since the beginning of cultivation Number of cells per 1 0 mg / l / = check / ml suspension 6-azido-6-deoxy 0.5 mg / L D-glucose 200 mg per liter of nutrient solution 0 7.0 χ 10 ⁴ 7.0 χ 10 ⁴ 7.0 χ 10 ⁴ 8 (2.9 + 0.09) x10 ⁵ (8.3 + 0.2) x10 ⁵ (8.0 + 0.1) x10 ⁵ 12 (2.8 + 0.1) x10 ⁶ (2.9 + 0.05) x10 ⁶ (3.8 + 0.06) x10 ⁶ 15 Dec (1.1 + 0.04) x10 ⁶ (2.2 + 0.08) x10 ⁶ (3.0 + 0.02) x10 ⁶

Thus, the composition of the invention allows an increase in productivity of the suspension cell culture by 36 to 225%. The composition according to the invention provides a stable increase in cell culture productivity throughout the second half of the culture cycle and even earlier, especially after the stabilization phase of the growth of the cell population has ended.

Claims (1)

An agent for increasing the number of cells and / or protoplasts in tissue and / or cell and / or protoplast culture, particularly effective in the second half of the culture cycle, characterized in that it consists of 6-azido-6-deoxy-D-glucose.