CS255205B1 - A means to reduce cell mortality - Google Patents

Abstract

The agent for reducing cell mortality in the final stages of the cultivation cycle of tissue, cell or protoplast cultures is formed by a cation of the general formula = ^-{(5}—COOH where R', R*1 is an alkyl of 1 to 6 C. The agent enables biochemical regulation and increased productivity of biotechnological production processes.

Description

The invention relates to the biochemical regulation of biotechnological production processes.

Biotechnological processes using tissue and/or cell and/or protoplast cultures still encounter difficulties associated with cell death at the end of the culture cycle. As can be seen from the following example, the use of a cation of the general formula

CH=N*H

COOH where R ¹ , R ^{1 1} is alkyl o. 1 to 6 C.

is based on the discovery of a new property of this compound, namely the ability to reduce the proportion of dead cells in the final phase of the culture cycle of tissue and cell cultures. The essence of the invention is a means for reducing the mortality of cells or/and protoplasts in the final phase of the culture cycle of protoplast and/or cell and/or tissue cultures, characterized in that it is formed by a cation of the general formula

CH = N ⁺ H COOH where R', R'' is alkyl of 1 to 6 C

This also achieves a technologically desirable increase in biomass production and an increase in the number of cells at the end of the cultivation cycle.

Example

Aqueous solutions freshly prepared from red crystals of para-(N-dimethyl-amino-benzylidene)-para-aminobenzoic acid sulfate were added to a standard nutrient solution for suspension cell cultures optimized for cells of the species Papaver somniferum (producer of opium alkaloids) before inoculation.

CH3 > ^N -^- ^CH=N+H - ^H ^ ^-COOH hso;

to achieve a concentration of the said compound of 0.5 mg per 1 liter of nutrient solution.

The initial cell concentration during inoculation was 70,000 per 1 ml of suspension. The cultivation cycle lasted 15 days. The following table shows the number of cells per 1 ml of nutrient solution on the last day of the cultivation cycle and the proportion of dead cells.

Cell count/ml Dead Cell Percentage (%) Day 15 cultivation cycle Check (1.1 ± 0.04) x 10 ⁶ 14.96 - 1.5 Addition of 0.5 mg of sulfate of the above formulas for 1 liter of nutrient solution (2.2 ÷ 0.07)xl0 ⁶ 2.06 - 0.47

It can be added to the above table that from the 10th day of the cultivation cycle, the composition according to the invention reduces cell mortality compared to the control. The composition according to our invention therefore provided in this particular case a 7 to 8-fold reduction in cell mortality at the end of the cultivation cycle. At the same time, biomass production was doubled.

Claims (1)

SUBJECT OF THE INVENTION A means for reducing cell mortality in the final stages of the culture cycle of tissue and / or cell and / or protoplast cultures, characterized in that it consists of a cation of the formula: R '. R: N - ^ - CH = N ⁺ H - ^ _ _COOH wherein R ', R' ¹ is alkyl of 1 to 6 carbon atoms.