CS250889B1 - Chlorotetracycline-producing Streptomyces aureofaciens CCM 3820 strain and resistant to phage infection - Google Patents

Abstract

The solution relates to a strain of the microorganism Streptomyces auritifiaciems CCM 3820 producing chlorotetracycline and resistant to phage infection.

Description

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The invention relates to the progenitor and Streptomycesaureofaciens CGM 3820 (internally designation is UV 712 / 84-31) prodrug chlorotetracycline, characterized by resistance to phage infection and stability of the properties of subsequent generations.

Increasing the industrial production of antibiotic chlorotetracycline is most often based on higher production ability of photosynthetic strains. These strains need to exhibit specific properties that they exhibit in fermentation in the plant. In particular, it maintains the high production ability and resistance to adverse effects in the fermentation phase, of which phage infection is the most dangerous.

Streptomyces aureofaciens strain CCM3820 hol obtained by a series of consecutive mutagenic interventions and passive selections from Streptomyces aureofaciensCCM 3766.

Other Ikmeňmii in genealogy, holiCCM line 3767 and tribe designationUV 71 / 84-15. UV light, a 214 µm dose, followed by selection in liquid apevnoin medium containing intrinsic meta-foolite-chlorotetracycline at a concentration of 170 and 670 µg in milliliter medium was conducted on this strain. The obtained sporulated colonies of the booster vaccinated sigars with standard sporulation. The spore cultures were stored at + 4 ° C for 20 weeks. One-third of the cultures retained the above-mentioned conditions. These cultures tested for production activity according to the spore system by fermentation and obtained from the strain Streptomycesaureofaciens CCM 3820, which is claimed. ·

The strain is characterized by the obtained, preactinophage B1 unfavorable host properties and high antibiotic production which reaches under specified conditions of cultivation. The shape of the colonies grown on the spoil, the monospore sowing, their pigmentation and the color of the spores are the same as those of the initial strain. CCM 3766.Rast strain CCM 3820 is slower on both solid and liquid soils than on the ground.

Microscopic preparations of culture from ferentation of strain CCM 3820 are characterized by thin, short and rich branched strands. The spores of strain CM 382.0 a, cultivation of its vegetative form, require observance of the specified culture conditions. The following table shows a comparison of the strain characteristics with the CCM 3766 and CCM 3767 production strains.

Strain Production, and chlortetracycline in foank Evidence of phage in culture ((Ug. Ml-1) (%) (° / oz number of cultures) CCM 3766 4 900 100 '79 CCM 3767 5 200 106 50 CCM 3820 (UV 72 / 84- 31) 5,400,110 less alcohol 2 Example 1

According to the prior art cultivation process, the strain is vegetatively inoculated in a 500 ml boiling pot with a 60 ml inoculum broth containing basic nutrients, namely sucrose as a source of carbon, corn liquor, soybean and ammonium salt as the nitrogen source of calcium carbonate. The soil for the preparation of the inoculum is inoculated with spores from one-third of the culture on slanted agar and not incubated for 22 hours.

The vegetative inoculum is seeded with a 500 ml flask with 60 ml of production broth. The source of carbon in this substrate is sucrose, the sources of nitrogen are corn liquor, soy flour and ammonium salt. The soil further comprises calcium carbonate, foenzyltethanate mineral salts. The preparation of the ivegetic inoculum and the fermentation are run on a rotary shaker at 240 rpm for an eccentricity of 2.5 cm at a cultivation temperature of 29 ° C. The fermentation lasts for 4 days. Samples for the determination of antibiotic production are taken at the 96th hour when the highest production rate is reached. By the above process, the production of chlorotetracycline is 4,700-5,200 µg. ml-1 soil. Example 1

According to the prior art cultivation of theproduct strain in Example 1, except that the preparation of the vegetative inoculum and fermentation proceeds in a large-scale camera. The inoculum is prepared in an inoculum tank with a volume of 5 m 3, which is filled with 3 m 3 of broth. The vegetative seed is seeded with a production nutrient fermenter of 50 m3, filled with 29 m3 of nutrient broth. Benzyltethanate is fed at 1 to 1.5 (µg / ml) together with a feed solution, where the feed starts when the carbon source is consumed to a level of 26 to 25 mg.

Claims (2)

OBJECT OF THE INVENTION A strain of Streptomyces aureofaciens CCM382O that prolongs chlorotetracycline and is resistant to phage infection.