CS247966B1 - Active ingredient of cosmetic and therapeutic agents - Google Patents

Abstract

The subject of the solution is an active ingredient of cosmetic and therapeutic products consisting of a mixture of biologically active substances obtained by heating a suspension of yeast cells with a concentration of 5 to 25% by weight, at a temperature of 30 to 75 °C for 5 to 25 hours at a pH of 6.5 to 8.5, in the presence of ethanol in a concentration of up to 7% by volume and subsequent centrifugation and washing of the sediment with water, and this ingredient is particularly suitable where it concerns the nutrition of mechanically or physico-chemically damaged skin, e.g. after irradiation with UV rays. In cosmetics, this ingredient can be used as a part of creams, ointments, skin milk and other compositions that have a beneficial effect on the skin, especially with frequent use.

Description

The invention relates to an active ingredient of cosmetic and medicinal preparations. When acting on the skin, physiologically active substances are used that support regeneration or provide improved nutrition.

The group of substances supporting skin regeneration includes, in particular, hormonal preparations, proteolytic enzymes and possibly natural polysaccharides. The components providing improved skin nutrition primarily include vitamins, proteins, peptides, amino acids and lipid substances such as sterols, essential fatty acids, etc.

It is known, for example, a composition for pharmaceutical, dermatopharmaceutical or cosmetic use containing peptides (mol. weight 10,000 to 60,000) obtained by hydrolysis of elastin (NSR Pat. 6. 2723 908) or the use of peptides (mol. weight 300 to 500) obtained by hydrolysis of substances containing collagen or gelatin for cosmetic purposes (British Patent No. 1590 656).

Amino acids are also used in cosmetics (Japanese patent No. 56-16766) and the role of essential and semi-essential amino acids in faster wound healing is particularly emphasized (British patent No. 20 92 911). Heat-treated (203 to 298 °C) protein waste from the food industry (Japanese patent No. 56 164 117) and other substances such as glycolipids (US patent No. 42 97340) or phospholipids together with ubiquinones (French patent No. 24 723 84) are used as cosmetic preparations.

The active substances may include deoxyribonucleic acids or their grafts from bacteria of the genus Bifidobacterium (German Federal Republic patent No. 302 43 18). The subject is the cultivation conditions and the composition of creams containing up to 10% of this active substance.

The use of polysaccharides from Scherotinium roelfsif biomass is also known (US patent no.

365 9025) and the use of phenol-water extraction of material containing yeast walls with the use of the extract in pharmacy (German Patent No. 245 2303).

In all cases, one is talking about the use of one more or less purified component of biological material, often of quite exclusive origin. This situation reflects the fact that so far it has not been possible to use the action of all described (and other) components simultaneously due to the insufficiently known physiological action and mainly also due to the mutual inactivation of the components of the multi-component mixture as a result of... artificial mixing. Also, the high price and complex preparation of some preparations prevent their wider spread.

These disadvantages are eliminated by the active ingredient of cosmetic and medicinal products, the essence of which is that it consists of a mixture of biologically active substances obtained by processing a suspension of the yeasts Secharomyces cerevitiae, Candida guilliermondii and Sacharomyces carlobergensis with a concentration of 5 to 25% by weight, at a temperature of 30 to 75 °C for 5 to 25 hours at a pH of 6.5 to 8.5, preferably in the presence of ethanol at a concentration of up to 7% by volume, with subsequent centrifugation and washing of the sediment with water.

The advantage of the process according to the invention is the possibility of using all active ingredients as a whole, without their inactivation, which leads to synergism of their action on tissues and, moreover, the possibility of obtaining the entire complex of active substances from the available raw material in a not very complicated way. The process according to the invention processes the cells of fungi or bacteria in such a way that all undesirable biomass beds are separated and the availability and activity of substances useful for the skin are increased. .

The product obtained according to the invention contains vitamins of Group B, amino acids, argosterol and other sterols. Furthermore, the product exhibits immunobilizing and skin tightening properties due to the presence of peptidoglycans and other cell wall polysaccharides.

The subject matter of the invention is illustrated, but not limited, by the following examples:

24796b

Example 1

To 500 ml of a 15% wt. aqueous suspension of S. cerevisiae yeast, 3% vol. ethanol was added and the suspension was maintained for 20 hours at a temperature of 50 °C (1 °G). The suspension was adjusted to pH 7. Then the suspension was separated and the sediment was washed twice with distilled water. After lyophilization of the sediment, 30.9 g of dry powdery product was obtained. The product was used to prepare a dermatological cream.

g of the powdered product was suspended in 3 g of dimethyl sulfoxide and 17 g of water, the mixture was heated to a temperature of 70 °C and slowly added with stirring to a mixture of 40 g of lenolin and 20 g of vegetable oil at a temperature of 70 °C. The resulting emulsion was cooled to laboratory temperature with stirring.

This cream was used to evaluate the activity in tissue regeneration processes. The activity was assessed by the rate of epithelialization of radiation ulcer scars in white Wistar rats, 12 per preparation; the irradiation dose was 500 rad.

TAB 1 shows the healing times of radiation scars with the product according to the invention compared to the skin preparation (Solkoseril). The healing time for the untreated control was 24 days = 100%: application of the ointment 3 times a day.

Table 1; Comparison of healing times of radiation scars treated with different agents

method of aDlication healing time 1 control 100% 2 Solcoseryl skin preparation 41% 3 cream 39% 4 shop without product from example 1 92% (116% ⁺ )

+ in two cases development of secondary infection

When using the cream, the healing time was shortened to 39% compared to the control, i.e. an average of 9.4 days.

Example 2

For the preparation of the product, Candida guilliermodii grown on n-paraffins was used and the procedure was continued according to Example 1.

g of the powder product from Candida guilliermondii was suspended in 2 g of ethanol and 33 g of water, the mixture contained 3 g of sorbic acid, 1 g of methionine and 1 g of cysteine at a temperature of 70 °C. This solution was added with stirring to 40 g of spermaceti heated to 70 °C. The resulting emulsion was cooled to laboratory temperature with stirring.

The cream has comparable effects to the cream from example 1.

Example 3

For the preparation of the product according to the procedure in Example 1, a suspension of Saccharomyces carlsbergensis yeast grown on the beer surface without removal of the entrained sludge was used.

g of the thus obtained powder product were suspended in 3 g of ethanol and 10 g of water and the suspension was added to a solution of 1 g of carboxyvinyl polymer in 72.4 g of water. After complete homogenization, 0.6 of triethanolamine in 10 g of water was added and the resulting gel was thoroughly mixed.

The gel nourishes and soothes sunburned skin. It does not contain any interfering oily ingredients.

2479o6

The physiologically active component according to the invention is suitable for the preparation of cosmetic and therapeutic agents, especially where it concerns the nutrition of mechanically or physico-chemically damaged skin, e.g. after UV radiation. In cosmetics, this component can be used as a part of creams, ointments, lotions and other compositions that have a beneficial effect on the skin, especially with frequent use.

Claims (1)

An active ingredient of cosmetic and therapeutic compositions, characterized in that it consists of a mixture of biologically active substances obtained by heating an aqueous suspension of yeast cells SachBromyces cerevitiae, Gandida guilliermondii and Sacharomyces carlsbergensis at a concentration of 5 to 25% by weight at 30 to 75 ° C for 25 hours at pH 6.5 to 8.5 in the presence of ethanol at a concentration of up to 7% by volume followed by centrifugation and washing the sediment with water.