CS247492B1 - Physiologically active component of cosmetics and remedies - Google Patents

Abstract

The subject of the solution is physiologically active component of cosmetic and therapeutic agents consisting of a biologically active mixture substances obtained by crushing yeast cells in an aqueous suspension of 3 to 20 wt. followed by centrifugation and washing sediment with water or weak liquor having a pH of 6.0 to 8.5 and concentration thereof; the ingredient is particularly suitable where it is skin regeneration damaged mechanically or physically chemically, e.g., after irradiation UV rays. In cosmetics, this component can be used used as part of creams, ointments, knits milk and other compositions that affect especially when used frequently, favorably to the skin.

Description

The invention relates to a physiologically active ingredient of cosmetic and therapeutic compositions.

Physiologically active substances support the regeneration of the skin or provide improved nutrition. In particular, hormone preparations, proteolytic enzymes and, optionally, natural polysaccharides belong to the group of skin regeneration promoters.

Ingredients providing improved skin nutrition primarily include vitamins, proteins, peptides, amino acids and lipid substances such as sterols, essential fatty acids, and others.

For example, compositions for pharmaceutical, dermatopharmaceutical or cosmetic use containing peptides / mol are known. weight 10,000 to 60,000 (obtained by hydrolysis of elastin). No. 2723 908) or the use of peptides / mol. weight 300-500 (obtained by hydrolyzing collagen or gelatin containing substances for cosmetic purposes (British Patent No. 1590,656)).

Amino acids are also used in cosmetics (Japanese Patent No. 5616766) and the role of essential and semi-essential amino acids in faster wound scarring is particularly emphasized (British Patent No. 20 82 911).

Heat treated (203 to 298 ° C) protein wastes from the food industry (Japanese Patent No. 56,164,117) and other substances such as glycolipids (US Patent No. 42,97340) or phospholipids together with ubiquinones are used as cosmetic preparations. franc. No. 24,723,884.

The active ingredients may comprise desoxyribonucleic acids and / or their grafts from Bifidobacterium (NSR Patent No. 302 43 18). The subject of the patent are the cultivation conditions and the composition of creams containing up to 10 ΐ of this active substance.

It is also known to utilize polysaccharides from the biomass of Scherotinium roelfies (U.S. Pat. No. 365 9025) and the use of phenol-aqueous extraction of yeast-containing material using pharmaceutical extract (German Patent No. 245 2303).

In all cases, it involves the use of one more or less purified component of biological material, often of rather exclusive origin. This situation reflects the fact that so far it has not been possible to utilize the action of all the described (and other) components simultaneously because of the poorly known physiological action and mainly due to the mutual inactivation of the components of the multicomponent mixture as a result of artificial mixing. Also, the high cost and complex preparation of some preparations prevent them from spreading more widely.

These drawbacks are overcome by the physiologically active ingredient of cosmetic and therapeutic compositions, characterized in that it consists of a mixture of biologically active substances obtained by crushing yeast cells in an aqueous suspension at a concentration of 3 to 20% by weight. and then centrifuging and washing the sediment with water or a weak lye at pH 6.0 to 8.5 and concentrating it.

The advantage of the process according to the invention is the possibility to use all active ingredients as a whole without their inactivation, which leads to synergism of their action on the tissues and furthermore the possibility to obtain the whole complex of active substances from the available raw material by a not complicated process. The process of the invention treat cells of yeast or bacteria in such a way that all undesirable biomass components are separated and the availability and activity of substances useful for the skin is increased.

The product obtained according to the invention comprises Group B vitamins, amino acids, ergosterol and other sterols. The product shows mild enzymatic activity, especially proteolytic and lipolytic, and has a significant effect on accelerating skin regeneration.

The invention is illustrated, but not limited, by the following examples.

He did

500 ml of a 10% aqueous suspension of Saccharomyces cerevisiae was crushed in a mechanical ballotin mill for 20 minutes. The homogenate was separated from the ballotins and adjusted to pH 7.2 with 10 min.

The sediment was washed again with distilled water under the same conditions (pH 7.2) and lyophilized after separation. 24.6 g of lyophilisate were obtained, which, after thorough grinding, was used to prepare a medicinal dermatological cream.

g of the powdered product was suspended in 3 g of dimethylsulfoxide and 17 g of water, the mixture was heated to 70 ° C and slowly added to a mixture of 40 g of lanolin and 20 g of vegetable oil at 70 ° C with stirring. The resulting emulsion was cooled to room temperature with stirring.

This cream was used to evaluate activity in tissue regeneration processes. Activity was evaluated by the rate of epithelialization of radiation ulcer scars in white wistar rats at 12 pieces per preparation; radiation dose was 500 rad.

In tab. 1 shows the healing times of radiation scars by the product according to the invention in comparison with the skin preparation (Solkoseril). The healing time of the untreated control was 24 days from: ~ 100%; application of ointment 3 times a day.

Table 1

Comparison of healing time of radiation scars treated by different means

Method of application Healing time 1. control 100% 2. Solkoseril skin preparation 41% 3. cream 26% 4. cream without the product of Example 1 92% / 115% ⁺ /

⁺ in two cases the development of secondary infection

Note: in the cream group, the hair was increased in 100% of cases. The control group remained bald. With Solkoseril, the increase was 83%.

Thus, using the cream reduced the healing time to 26% compared to the control, ie an average of 6.25 days.

Regenerating cosmetic milk was prepared from the same product.

g of the powdered active ingredient of Example 1 was suspended in a mixture of 63.7 g of water,

0.2 g of oxyethylenated fatty alcohol and 0.2 g of triethanolamine heated to 70-80 ° C. An equally warm mixture of 2 g of stearin, 1.5 g of cetyl alcohol, 4.95 g of oleic acid decyl ester, 0.05 g of azulene, 8 g of vaseline oil and 7 g of vegetable oil was added to this mixture with stirring. The resulting emulsion was cooled with stirring and 0.2 g of the fragrance composition was added at 30 ° C.

The resulting milk was used as a tanning product and a beneficial calming effect was found.

Example 2

Candida guilliermondia grown on n-paraffins was used to prepare the product and proceeded as described in Example 1.

The product was further used to prepare another dermatological cream.

g of the powdered product from Candida guilliermondia was suspended in 2 g of ethanol and 33 g of water, and 3 g of sorbic acid, 1 g of methionine and 1 g of cysteine were dissolved in the mixture at 70 ° C. This solution was slowly added with stirring to 40 g of spermaoet heated to 70 ° C. The resulting emulsion was cooled to room temperature with stirring.

The cream has similar effects to that described in Example 1 except that it additionally alleviates skin irritation of allergic origin.

Example 3

A yeast suspension of Saccharomyces carlsbergensis grown on beer wort without removal of entrained sludge was used to prepare the product and proceeded in the same manner as in Example 1. A regenerating cream was prepared from the product.

g of the powder product thus obtained were suspended in 3 g of ethanol and 10 g of water, and the suspension was added to a solution of 1 g of carboxyvinylpolymer in 72.4 g of water. After complete homogenization, 0.6 g of triethylamine in 10 g of water was added and the gel formed was thoroughly mixed.

Gel cream accelerates skin regeneration after sunburn and pigment formation by up to 48%. Free of interfering fatty component.

From the same product was prepared another cream with regenerative effects on the skin.

g of the powdered active ingredient of Example 3 was suspended in a mixture of 61.3 g of water, 5 g of glycerol and 0.4 g of triethanolamine. The mixture was heated to 75 ° C and mixed slowly with an equally warm mixture of g g of fatty acid monodiester in glycerol, 8 g of stearin, g of 2-octyldodecanol, 5 g of refined vegetable oil.

The resulting emulsion was cooled to room temperature with stirring. 0.3 g of fragrance composition was added at 30 ° C. The resulting emulsion was applied to the aging skin and beneficial regenerative effects were found.

The physiologically active ingredient according to the invention is suitable for the preparation of cosmetic and therapeutic compositions, in particular where the regeneration of skin damaged mechanically or physically-chemically, e.g. after irradiation with UV rays. In cosmetics, this ingredient can be used as part of creams, ointments, lotions and other skin-beneficial compositions.

Claims (1)

SUBJECT OF THE INVENTION A physiologically active ingredient of cosmetic and therapeutic compositions, characterized in that it consists of a mixture of biologically active substances obtained by crushing yeast cells of Sacharomyces cerevisiae, Candida guilliermondia and Saoharomyces carlsbergensis in an aqueous suspension at a concentration of 3 to 20% by weight. and then centrifuging and washing the sediment with water or a weak lye at pH 6.0 to 8.5 and concentrating it.