CS231145B1 - A method of producing a subcellular parenteral agent against neonatal colientitis and swine collagen - Google Patents

Abstract

The invention relates to a new method of isolating the pilus body antigen K 88 of the enteropathogenic bacteria Bscherichis. coli. The said isolated antigen is used either for the immunization of sows alone or as a supplement to other vaccines against intestinal inflammation and diarrhea in newborns. Due to its low production costs and simple handling, it is widely used in large-scale animal production, where it significantly contributes to reducing piglet mortality.

Description

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BACKGROUND OF THE INVENTION The present invention relates to a method for producing an effective subcellular perenteral agent against neonatal collageneritis and porcine diarrhea administered to dusty sows 6 weeks later by injection into a muscle or pre-episode.

Colienteritis and newborn pig colony are a serious problem for the large-bogged wheezing »Her most effective means of disposing of her is the immunization of newborn infants with an acute oral vaccine containing dead Escherichiacoli bacteria, which contains a follicular am 88 K 88, optionally entero-toxin »The method is to immunize the mothers against poro-comenary via the eelulum, or by the subcellular vaccine containing the whole enteropathogenic bacterium Escherichia coli, or the isolated Plus antigen 88 by the combination of both. continually incorporating new and new strains of production and daily manual administration of vaccine to a number of thousands and thus marking the fatigue work · Parenteral, cellular or subcellular the vaccines immunize the mother and the pig after the infestation with the colostrum against antimicrobials K 88 · So far, the parenteral - 2,231,143 vaccines produced have been prepared in very demanding and expensive ways, requiring several steps of isolation with acetic acid, ammonium sulphate and expensive equipment , such as < RTI ID = 0.0 > < / RTI >

The above-mentioned drawbacks are substantially eliminated by the production of a subcellular parenteral agent against neonatal collageneritis and a pig cradle of the invention, which results in the K 88 pilus antigen from enteropathogenic Escherichia coli bacteria being separated by homogenization and isolated by isoelectric autofocusing to a concentration that exhibits a clear precipitating line with agar diffusion against antic 88 acer up to 1: 128 dilution * An advantage of the invention is the low cost of production, the ease of manipulation in animal immunization, which is single-dose and requires only maternal immunization. since the separation takes place only in pyrogen-free sterile distilled water * Example

The enteropathogenic bacterium Escherichia coli 8888 +, the nonscepilus antigen 8888, is centrifuged after normal culture to produce said antigen, and the non-supercoiled bacterium is resuspended at 4 ° C in cold sterile distillation using a 5-10 min at 3000 rpm homogenizer and warm to 4 ° C. Thus, the separation of the antigens containing antigen 88 takes place. Thereafter, the homogenate is centrifuged at 70,000 m 2 s-2 for 20 min and at 4 ° C *. The supernatant, whose conductivity is adjusted to values up to 800 µl by addition of sterile distilled water or further addition of the K 88 antigen, is applied to the horizontal isoelectrofocusing large-capacity apparatus with mechanically stabilized split solutions. at a temperature of 1 ° C until the current drops below 3 mA at a voltage of 1,000 V · After focusing, the pH is adjusted in all the apertures of the apparatus. The K 88 pilus antigen focuses on pBL4.2 to 5.0 automatically · From pHv shelves this: ranges the fimbrial pilus antigen K 88 · Its pH is adjusted to 7 to 7.5 and the concentration is adjusted to show the agar precipitation clear precipitate against anti 88 88 to. to 1: 128 dilution · Achieving this concentration with adjuvant, e.g. oily, adjuvanted to the antigen solution and administered parenterally by injection at a dose of 2 to 3 ml subcutaneously or int frame to susceptible sows about 6 weeks before delivery. anti-vytvořili 88 antigen antibodies are created in mothers and are transferred to the neonatal intestines after drinking. These antibodies prevent the entero-pathogenic Escherichia coli bacteria from adhering to the intestinal wall and thereby cause colienteritis and coli-diarrhea.

The invention can be used to find widespread practical use in animal production in large-scale farming, where it contributes significantly to the reduction of newborn infants due to coli-enteritis and cystitis.

Claims (1)

A process for the production of succinular parenteral anti-neonatal colleeateritis and a scurvy pimper, characterized in that pilus aafcigen K 88 is separated from the body surface of entero-pathogenic Escherichia coli by homogeniation and isolated by aggression by diffusion by diffusion by de-electric decomposition have a car k88 up to 1: 128 dilution ·