CS223545B1 - Detergent enriched with microbial proteases, especially alkaline - Google Patents

Abstract

In addition to the usual components, the detergent according to the invention contains proteases produced by the cultivation of mutant strains of Bacillus licheniforátis, Laktoflora δ. 974 and 975. The detergent enriched in this way acts on a wider range of chemically different dirt, has high stability and removes dirt, especially of organic origin.

Description

The invention relates to a detergent enriched with proteases, predominantly alkaline, produced by cultivation of mutant BaciHus lioheniformis, Lactoflora Nos. 974 and 975 strains.

The use of microbial proteases in detergent production is known. It is known to use proteases produced by some strains of Baeillue subtilis and Bacillus megaterium for the same purpose.

A drawback of the present state is that the doeud protease preparations prepared have low potency, contain, in addition to the active alkaline proteases, a considerable amount of neutral and acid proteases, with some chemically different impurities being leader resistant.

These drawbacks are remedied by the detergent composition of the present invention, which consists essentially of alkali-type proteases produced by mutants of Bacillus lioheniformis, Lactoflora Nos. 974 and 975. These enzymes are applied to the detergent composition after separation and concentration. The detergent composition is produced in various forms such as, for example, a detergent powder, a detergent concentrate, a low sudsing liquid detergent, and the like.

The protease enzymes produced by the mutants of the invention act on most of the chemically distinct impurities, thereby increasing the efficiency of detergents.

The protease enzymes of the invention are harvested after fermentation by separation from nutrient medium and living cells to mutants of Bacillus lioheniformis, Lactoflora or 974 and 975 strains using filters and centrifuges. Alternatively, the proteases are purified and concentrated, dft detergents are added in liquid form, concentrated by concentration, or in the form of a dried protease preparation.

The increased effect of the detergent composition according to the invention is caused

- 2,223,545 in that the bred mutants of B. lichiniformis, Lactoflora Nos. 974 and 975 have high production of active proteases and the proteases produced are predominantly alkaline, so that they intensively affect a variety of chemically distinct impurities. These proteases have a high stability in the wash solution, thereby guaranteeing a permanent effect in removing impurities, in particular of organic origin.

Example

The detergent powder comprises 12.8 wt. sodium alkylarylsulfonate, 2.2 wt. of a copolymer of polyethylene oxide and polypropylene oxide, 4.5 wt. soap, 12 wt. sodium tripolyphosphate, 6 wt. water glass solids, $ 27 wt. sodium carbonate, 30 wt. % sodium sulfate, 1.5 wt. % carboxymethylcellulose, 0.3 wt. optically brighteners, 0.2 wt. perfume and 0.5 wt. microbial proteases produced by mutants of Bacilius licheniformis, Lactoflora Nos. 974 and 975, in stabilized form at a concentration of 300,000 IU / g.

The alkaline protease-enriched detergent composition of the present invention acts on a wider range of chemically different impurities compared to known detergents, has a higher stability and removes impurities especially of organic origin.

Claims (1)

Detergent enriched with microbial proteases * particularly alkaline, characterized in that it contains, in addition to the usual components, microbial proteases produced by mutants of Bacillus licheniformis, Lactoflora Nos. 974 and 975 ·